Thermo Scientific Transcend Multichannel UHPLC Systems

Thermo Scientific™ Transcend™ Multichannel UHPLC Systems are advanced LC-MS platforms designed for laboratories that demand higher throughput, improved efficiency, and stronger return on investment. Their multichannel UHPLC architecture connects up to four fully independent LC channels to a single mass spectrometer, dramatically increasing system utilization without compromising data quality.

Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: The Transcend LX systems feature either 2 or 4 (shown) completely independent LC channels.

Key benefits

• Significantly increased LC-MS throughput
• Maximized mass spectrometer utilization
• Maintained sensitivity, selectivity, and robustness
• Reduced operating costs and analysis time
• Scalable configurations tailored to laboratory needs

Multichannel UHPLC architecture

Conventional single-channel LC-MS systems process samples sequentially, leaving the mass spectrometer idle during injection, column washing, and equilibration.
Transcend systems overcome this limitation by intelligently interleaving injections from multiple LC channels, delivering data to the mass spectrometer only during relevant retention time windows and enabling substantial productivity gains.

Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: Injections are interleaved to maximize throughput and mass spectrometer use.

Vanquish UHPLC platform

Thermo Scientific™ Transcend™ Multichannel UHPLC Systems are built on proven Thermo Scientific Vanquish UHPLC technology. The Transcend multichannel architecture integrates up to four independent Vanquish-based UHPLC channels into a single LC-MS platform.

The Vanquish UHPLC platform provides:

• high mechanical and operational robustness
• reproducible long-term performance
• precise gradient formation and stable flow control suitable for multichannel operation
• flexible pump configurations (binary or quaternary) tailored to application requirements

By combining the Transcend multichannel concept with Vanquish UHPLC technology, laboratories can achieve high throughput without compromising chromatographic performance, sensitivity, or system reliability.

System configurations

• Transcend LX and VLX
  Designed for high-throughput LC-MS workflows without on-line sample cleanup
  • 2- and 4-channel configurations
  • Fully independent LC channels

Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: Transcend LX UHPLC Systems

• Transcend TLX
  Designed for workflows requiring automated on-line sample cleanup
  • 1-, 2-, and 4-channel configurations
  • Integrated TurboFlow™ technology

Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: Transcend TLX UHPLC Systems

Each LC channel operates independently, allowing identical methods to run in parallel or multiple methods to be combined within a single system.

On-line sample cleanup with TurboFlow™ technology (TLX)

TurboFlow™ technology enables rapid on-line removal of matrix interferences directly within the LC-MS system, resulting in:

• Reduced or eliminated manual sample preparation
• Direct injection of complex biological and environmental samples
• Improved sensitivity and reproducibility
• Increased method robustness and simplified workflows

Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: On-line sample cleanup with TurboFlow™ technology (TLX).

System control and software

All system functions are managed by Thermo Scientific™ Aria™ MX software, providing:

• Control of pumps, valves, gradients, and injection scheduling
• Intuitive multichannel method setup
• Parallel execution of up to four methods
• A user-friendly graphical interface for complex LC-MS workflows

Aria MX software is fully compatible with Thermo Scientific™ Xcalibur™ and Thermo Scientific™ TraceFinder™ software.

Mass spectrometer compatibility

Transcend systems are designed as a flexible LC-MS platform compatible with a broad range of Thermo Fisher Scientific mass spectrometers, including:

• TSQ triple quadrupole systems
• High-resolution Orbitrap instruments

Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: Transcend TLX-2 system with a TSQ Altis Plus triple quadrupole mass spectrometer.

Typical application areas

• Clinical research and bioanalysis
• Pharmaceutical development and quality control
• Toxicology
• Environmental analysis
• High-throughput screening workflows

Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: Retention time (A) and peak area (B) reproducibility of a high-throughput targeted proteomics assay for quantifying SARS-CoV-2 peptides in clinical research samples across five days. The Transcend TLX-4 UHPLC system was used for on-line sample clean-up and analysis of 500 respiratory tract swab samples per day. (Cardozo, K.H.M., Lebkuchen, A., Okai, G.G. et al. Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts. Nat Commun 11, 6201 (2020))