For researchers pursuing the next scientific breakthrough, the Thermo Scientific™ Vanquish™ Neo UHPLC system offers the highest performance, productivity and usability of any nano-, capillary- and micro-flow UHPLC system.
Discover more in every sample with excellent system performance and unmatched retention time precision for shallow gradients, high-throughput applications and anything in between. Vanquish Neo is the first UHPLC system built not only to operate, but to excel across the entire low-flow range ideal for LC-MS applications.
Thermo Scientific™ Vanquish™ Neo UHPLC System: The Vanquish Neo UHPLC system delivers flow rates from 1 nL/min to 100 µL/min to support a broad range of high-sensitivity LC-MS workflows. Sensitivity gain (red trace) relative to a 2.1 mm I.D. column operated at 450 µL/min corresponds to increased electrospray ionization efficiency at lower LC flow rates. The Vanquish Neo UHPLC system permits maximum sample throughput for a broad range of low-flow columns and applications (colored bars). * Within specifications for retention time precision (≤0.2 % RSD or ≤0.1 SD)
Ideal for micro-flow LC-MS applications, the optional Vanquish Neo column compartment provides stable temperature control for linear columns, coiled capillary columns and trap columns.
From maximizing peptide identifications in discovery proteomics to high-throughput precision medicine, the Vanquish Neo UHPLC system was designed with the versatility to tackle all your high- sensitivity analyses.
Thermo Scientific™ Vanquish™ Neo UHPLC System.
Thermo Scientific™ Vanquish™ Neo UHPLC System: The Vanquish Neo UHPLC system supports a broad range of flow rates and gradient lengths without any changes to pump hardware. For all runs, 1 µg of HeLa protein digest was separated on a Thermo Scientific™ EASY-Spray™ PepMap™ Neo column by a Vanquish Neo UHPLC system interfaced to a Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer operated in data dependent acquisition mode.
In your lab, system performance and productivity shouldn’t be at odds. The Vanquish Neo UHPLC system enables you to consistently generate high-quality data while maximizing MS utilization.
Thermo Scientific™ Vanquish™ Neo UHPLC System: Fast sample loading and equilibration increase system productivity.
Thermo Scientific™ Vanquish™ Neo UHPLC System: Comparison of sample throughput with and without fast sample loading and column equilibration. Estimated increase in sample throughput ranges from 18–81%, depending on column dimensions.
In addition to fast sample loading and equilibration, the Vanquish Neo UHPLC system tandem direct injection workflow pushes your analyses even further by eliminating method overhead. Utilizing a two-pump, two-column configuration, the workflow performs column loading, washing and equilibration offline and in parallel to the analytical gradient to offer:
Thermo Scientific™ Vanquish™ Neo UHPLC System: The tandem direct injection workflow operates using a second flow path for offline column washing, equilibration and loading.
Thermo Scientific™ Vanquish™ Neo UHPLC System: The instrument method editor wizard, available with Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) and Thermo Scientific™ Standard Instrument Integration (SII) for Xcalibur, provides automatic parameter population and determination of method execution timings to help ensure seamless transfer of methods to tandem direct injection.
Built from the ground up in order to redefine the low-flow chromatographic system, the innovative Thermo Scientific™ Vanquish™ Split Sampler NT provides superior sample injection performance.
Thermo Scientific™ Vanquish™ Neo UHPLC System: Injection volume linearity of a caffeine standard from0.1 to 1.0 µL using flow injection analysis.
Thermo Scientific™ Vanquish™ Neo UHPLC System: Carryover for 15 PRTC peptide standards spiked into HeLa protein digest. 200 ng was injected onto a 75 µm I.D. × 75 cm column followed by a blank injection using a Vanquish Neo UHPLC system coupled to an Orbitrap Exploris 480 operated in data dependent acquisition mode. Percent carryover is listed above the peak area for each blank injection. The flow rate was 250 nL/min during the separation phase.
Modern proteomics, lipidomics, metabolomics, translational research and bioanalysis depend on LC-MS instrumentation capable of identifying and quantifying subtle analyte concentration differences across large sample cohorts. The Vanquish Neo UHPLC system instills confidence in your results by delivering efficient separations 24/7 without compromising robustness.
Thermo Scientific™ Vanquish™ Neo UHPLC System: 8 peptides were selected from a 90 minute gradient separation of 1 pmol BSA protein digest performed at 300 nL/min with the system configured in direct injection mode (A). Retention time stability was monitored for the selected peptides over a period of 176 days. In total, 1600 injections were performed using 1500 bar system pressure for sample loading and column equilibration (B).
Every Vanquish Neo UHPLC system is designed and rigorously tested to guarantee high-quality separations and system-to-system reproducibility, both within labs and around the globe. Standardized configurations improve your user experience throughout the instrument’s lifetime.
Thermo Scientific™ Vanquish™ Neo UHPLC System: Results of 200 ng HeLa protein digest profiling with 6 different Vanquish Neo UHPLC systems coupled to Thermo Scientific™ Orbitrap Exploris™ 240 systems. Used 75 µm × 50 cm, 2 µm EASY-Spray PepMap Neo column, 90 min gradient, 300 nL/min flow rate, direct injection, 1500 bar sample loading and column equilibration.
The Vanquish Neo UHPLC system is equipped with Viper and nanoViper fittings for leak-free operation up to 1500 bar, preventing time-consuming troubleshooting of system fluidics. Viper capillaries limit extra column dispersion by utilizing a PEEK tip seal and bushing instead of a traditional ferrule for improved separation performance. All 1500 bar nanoViper fittings are biocompatible and offer high capillary-to-capillary reproducibility.
The Vanquish Neo UHPLC system delivers uncompromised results to expert LC-MS users while expanding the toolbox for novice users for nano-, capillary- and micro-flow LC-MS applications. An intuitive user interface and intelligent system control help to make the Vanquish Neo UHPLC system the most user-friendly low-flow UHPLC system ever built. The Thermo Scientific™ Vanquish™ User Interface provides:
Thermo Scientific™ Vanquish™ Neo UHPLC System: Walk-up and remote system status monitoring.
The Vanquish Neo UHPLC system features a new level of system intelligence with inter-module communication. Automated injection routines with optimized parameters for sample loading and fluidics washing deliver high chromatographic performance and reproducibility. The instrument method editor wizard helps ensure column compatibility with separation parameters.
Thermo Scientific™ Vanquish™ Neo UHPLC System: The system interlink and single system driver provide an optimal user experience through intelligent, direct communication between modules, harmonizing all aspects of the chromatographic method execution.
Thermo Scientific™ Vanquish™ Neo UHPLC System: Enhanced ease of use, method robustness, and standardization through active integration of consumable information such as column dimensions, pressure and flow limits to help ensure methods adhere to consumable specifications.
Thermo Scientific™ Vanquish™ Neo UHPLC System: Factory pre-calibrated for common LC-MS mobile phases, plus guided calibration procedures for additional solvents (A). Simplified gradient programming guided by time and flow permits the generation of standardized methods based on gradient or column volume (B). Reduced user errors with active notifications which appear when operating out of the recommended range for method parameters such as flow rate and column washing/equilibration times (C).
Thermo Scientific™ Vanquish™ Neo UHPLC System.
Provides smart system operation through system status monitoring, automatic start-up procedures and direct instrument control without requiring a PC connection for high operational convenience.
Straightforward fluidic design with labeled lines and pre-defined solvent bottle positions in the solvent rack.
Reduces pressure drop for both direct and trap-and-elute injection, yielding high retention time precision and extending column lifetime.
Accurate injection volumes from 10 nL to 100 μL. Sample multiple-draw functionality extends the injection range up to 500 μL for sample analysis using the trap-and-elute workflow.
Choose between direct injection, tandem direct injection and trap-and-elute workflows. For trap-and-elute, switch between forward-flush or backward-flush modes without replumbing fluidics.
Negligible system carryover through inner and outer injection needle and fluidics wash routines automatically executed in parallel to the sample analysis.
Injection from limited sample volumes with near zero sample loss (up to 2.5 out of 3 μL).
Nano-, capillary- and micro-flow LC at up to 1500 bar. Leak- free nanoViper fittings minimize extra column dispersion and GDV, maximizing throughput and separation quality.
Active flow control across the nano-, capillary- and micro-flow ranges and factory multipoint flow calibration deliver industry-leading gradient accuracy, gradient precision and system-to-system reproducibility.
Accelerated sample loading and column equilibration with increased flow rate and pressure, reducing analysis time.
Direct inter-module communication provides superior user experience and system intelligence.
A practical drawer allows the storage of consumables and tools for user convenience.
Smart guided method creation linked to consumable and fluidic parameters helps ensure robustness through optimal operation, fast sample loading and sufficient column washing and equilibration.
Thermo Scientific™ Vanquish™ Neo UHPLC System.
Temperature-controlled direct injection or heated trap-and-elute injections provide increased system versatility.
Thermo Scientific™ Vanquish™ Neo UHPLC System.
Built alongside the 1500 bar-capable Vanquish Neo UHPLC system, Thermo Scientific™ nano-, capillary- and micro-flow columns enable simple, tool-free setup and configuration. Thermo Scientific™ PepMap™ Neo columns range from 75 μm to 1 mm I.D., while Thermo Scientific™ μPAC™ Neo columns excel at nano and capillary flow rates with lengths ranging from 5.5 to 110 cm. PepMap Neo and μPAC Neo columns improve separation performance across the entire Vanquish Neo UHPLC system flow range, making them ideal for LC-MS applications.
Thermo Scientific EASY-Spray PepMap Neo columns help ensure robust nano- and capillary-flow LC-MS analysis. Available in 75 and 150 μm, the EASY-Spray integrated column and emitter design virtually eliminates dead volume and is temperature-controlled for maximum reliability and performance.
In addition to excellent chromatographic performance, μPAC Neo columns offer a unique combination of robustness and reproducibility across large sample sets.
Trap columns help accelerate sample loading and permit online sample concentration and desalting as well as helping to ensure protection of our entire low-flow column portfolio. The Thermo Scientific™ PepMap™ Neo trap is compatible with both PepMap and uPAC columns, rated to 1500 bar, and supports forward and backward flush modes. Trap columns are also available in 75 and 100 μm I.D.
Standalone columns for nano- and capillary-flow are designed with single nanoViper and double nanoViper trouble-free connectors for robust separation. Available in 75 and 150 μm and compatible with any MS system source design, they deliver excellent resolution, long column lifetime and low carryover.
For outstanding peak shapes in micro-flow chromatography, 300 μm and 1 mm I.D. columns are available in a range of chemistries.
Nano and capillary emitters act as a column-independent sprayer, allowing the introduction of flow from nano and capillary columns without the troublesome handling of traditional emitters and connectors.
The Vanquish Neo UHPLC system is designed for a wide range of high-sensitivity LC-MS applications and flawless integration with industry-leading Thermo Scientific mass spectrometry portfolio and ESI sources.
Thermo Scientific™ Vanquish™ Neo UHPLC System.
Thermo Scientific LC portfolio overview.
