<p><strong>🎤Dr. Leigh Terry (University of Alabama)</strong></p><p><strong>In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.</strong></p><p>While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.</p><h4><strong>In this episode, we cover:</strong></h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted<strong> LC-MS/MS methods</strong></li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (<strong>CIC</strong>) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4><strong>Key Insight:</strong></h4><p>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.</p><h3><strong>Video Transcription</strong></h3><p>In this episode of <i>Concentrating on Chromatography</i>, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.</p><h4><strong>Understanding PFAS and Their Significance</strong></h4><h5><strong>The University of Alabama Water Quality Research Center</strong></h5><p>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.</p><p>The laboratory specializes in:</p><ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul><p>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.</p><h4><strong>Why Focus on PFAS?</strong></h4><p>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.</p><p>According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:</p><ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul><p>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.</p><h4><strong>The Development of Standardized PFAS Methods</strong></h4><p>One major advancement in PFAS analysis has been the development of standardized EPA methods.</p><p>These methods allow:</p><ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul><p>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.</p><h4><strong>How Large Is the PFAS Universe?</strong></h4><p>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.</p><p>PFOA and PFOS are often referred to as:</p><ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul><p>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.</p><p>Researchers estimate:</p><ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul><p>Scientific debate continues regarding classification and definition, making precise counting difficult.</p><h4><strong>Why PFAS Are Called "Forever Chemicals"</strong></h4><p>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.</p><p>PFAS persistence results from two key chemical properties.</p><h5><strong>1. Amphiphilic Structure</strong></h5><p>PFAS molecules contain:</p><ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul><p>This unusual combination allows PFAS to partition between:</p><ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul><p>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.</p><h5><strong>2. Extremely Strong Carbon–Fluorine Bonds</strong></h5><p>The carbon–fluorine bond is among the strongest bonds in organic chemistry.</p><p>As Dr. Terry explains:</p><ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul><p>These properties make PFAS extremely persistent in both environmental systems and biological organisms.</p><h4><strong>Toxicity, Regulations, and Environmental Behavior</strong></h4><h5><strong>Understanding PFAS Toxicity</strong></h5><p>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.</p><p>Dr. Terry explains that EPA regulations generally consider three factors:</p><ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul><p>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.</p><p>This creates important scientific questions:</p><ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul><p>These uncertainties continue to drive active research.</p><h4><strong>What Happens to PFAS in Wastewater Treatment Plants?</strong></h4><p>PFAS do not behave like many conventional contaminants.</p><p>Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:</p><ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul><p>As a result:</p><ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul><p>Their distribution depends on wastewater composition and treatment processes.</p><h4><strong>Regulatory Monitoring and Targeted LC-MS Analysis</strong></h4><p>Current EPA regulations focus on targeted analysis of specific PFAS compounds.</p><p>Examples include:</p><ul><li>PFOA</li><li>PFOS</li></ul><p>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.</p><p>Advantages of targeted LC-MS analysis include:</p><ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul><p>However, limitations remain:</p><ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul><p>As Dr. Terry notes, targeted analysis provides only part of the overall picture.</p><h4><strong>The Fluorine Mass Balance Problem</strong></h4><p>One of the most important concepts discussed in the interview is fluorine mass balance.</p><p>When PFAS degrade, fluorinated fragments may remain in the environment.</p><p>This creates a critical question:</p><p>Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?</p><p>To answer this, researchers perform fluorine mass balance calculations using:</p><ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul><p>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.</p><h4><strong>Advanced Analysis, Treatment Technologies, and Future Directions</strong></h4><h5><strong>Combining Targeted and Bulk Fluorine Measurements</strong></h5><p>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.</p><p>Approaches include:</p><ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul><p>In these methods:</p><ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul><p>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.</p><h4><strong>Surprising Findings from Combined Analysis</strong></h4><p>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.</p><p>Dr. Terry reports that:</p><ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul><p>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.</p><h4><strong>Preventing PFAS Contamination in the Laboratory</strong></h4><p>PFAS analysis requires exceptionally strict quality control.</p><p>Dr. Terry's laboratory routinely:</p><ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul><p>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.</p><h4><strong>Current PFAS Treatment Technologies</strong></h4><p>Three technologies currently dominate PFAS removal in drinking water treatment:</p><ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul><p>Selection depends on:</p><ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul><p>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.</p><h4><strong>Membrane Filtration and Pretreatment</strong></h4><p>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.</p><p>Her own work focuses on pretreatment through biological filtration.</p><p>The strategy involves:</p><ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul><p>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.</p><h4><strong>Regional Monitoring Projects</strong></h4><p>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.</p><p>Their observations indicate:</p><ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul><p>Examples include:</p><ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul><p>Source identification remains a critical component of environmental monitoring programs.</p><h4><strong>Future Directions for PFAS Monitoring</strong></h4><p>Looking ahead, Dr. Terry sees several opportunities for improvement.</p><p>Desired advances include:</p><ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul><p>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.</p><h4><strong>Dr. Terry's Perspective on Long-Term Solutions</strong></h4><p>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.</p><p>Rather than focusing solely on remediation, she emphasized prevention.</p><p>Her view is that society should:</p><ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul><p>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.</p><h4><strong>Final Thoughts</strong></h4><p>Dr. Terry encourages consumers to:</p><ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul><p>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.</p><h4><strong>Conclusion</strong></h4><p>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.</p><p>The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

<p><strong>This study evaluates an immobilized enzyme reactor (IMER) containing peptide N-glycosidase from </strong><i><strong>Rudaea cellulosilytica</strong></i><strong> (PNGase Rc) for rapid online deglycosylation of intact glycoproteins. Human glycoproteins and therapeutic antibodies were used as model substrates to assess reactor performance.</strong></p><p>The PNGase Rc IMER efficiently removed complex sialylated and core-fucosylated glycans, simplifying protein characterization and glycosylation-site analysis. The approach offers a fast and effective solution for bioanalytical workflows, biopharmaceutical quality control, and stability testing of therapeutic proteins.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577"><strong>Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor</strong></a></h4><p>Katarína Molnárová, Petr Novák, Jana Nováková, and Petr Pompach*</p><p>ACS Omega 2026, 11, 17, 25724–25729</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.6c00577">https://doi.org/10.1021/acsomega.6c00577</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>Protein glycosylation, in which monosaccharide units are attached to a protein backbone through enzymatic pathways, is involved in many important biochemical processes and is considered one of the most important post-translational modifications. <a href="javascript:void(0);">(1)</a> It affects not only the stability and secretion of proteins, cell–cell interactions, and receptor–ligand recognition mechanisms but also the biological activity, plasma half-life, immunogenicity, pharmacodynamics, and pharmacokinetics of biotherapeutics. <a href="javascript:void(0);">(2−5)</a> Therapeutical monoclonal antibodies (mAbs) are among the best known pharmaceuticals that are based on glycoproteins. <a href="javascript:void(0);">(6)</a> Over 90% of the mAbs are derived from immunoglobulin G (IgG), in which <i>N</i>-glycosylation occurs at Asn297 of the Fc region and is responsible for modulating immune responses and interactions with other proteins. <a href="javascript:void(0);">(7,8)</a> In cancer treatment, mAbs are used to increase the response of the immune system against the tumor cell, thus inhibiting the cancerous cell growth and helping with the destruction and elimination. <a href="javascript:void(0);">(9)</a> Among the most commonly used mAbs are trastuzumab, bevacizumab, cetuximab, or margetuximab, which are used as an additional treatment for breast, colorectal, prostate, or lung cancer. <a href="javascript:void(0);">(10)</a></p><p>Mass spectrometry (MS)-based methods have become a method of choice in the analysis of mAbs that can provide information on sequence, post-translational modifications, and glycosylation profiles. <a href="javascript:void(0);">(11)</a> The enzymatic removal of the attached glycans from the protein backbone can reduce the complexity of the MS analysis, which can lead to a better understanding of the protein structure and modifications. In glycoproteomics, peptide-<i>N</i>-glycosidase F (PNGase F) is most used for glycan removal. It cleaves the linkage between the Asn residue and the <i>N</i>-acetylglucosamine (GlcNAc). <a href="javascript:void(0);">(12)</a> Jmeian et al. developed a monolithic PNGase F enzyme reactor fabricated in fused silica using a glycidyl methacrylate-<i>co</i>-ethylene dimethacrylate polymer and integrated it into an LC–MS workflow. The authors demonstrated the applicability of this system for the characterization of released neutral and acidic <i>N</i>-glycans by LC–MS analysis. <a href="javascript:void(0);">(13)</a> In another study, oriented immobilization of PNGase F onto a methacrylate-based monolithic support via glutathione-<i>S</i>-transferase–glutathione affinity coupling was employed to release <i>N</i>-glycans from denatured ribonuclease B and fetuin, as well as from native IgG. The released glycans and the corresponding deglycosylated proteins were subsequently analyzed by MALDI-MS. <a href="javascript:void(0);">(14)</a></p><p>Gramlich et al. showed the potential of PNGase Rc for <i>N</i>-glycan hydrolysis from both tryptic peptides and native glycoproteins at a low pH. The favorable properties of PNGase Rc, including activity at acidic pH and compatibility with reducing agents such as <i>tris</i>(2-carboxyethyl)phosphine hydrochloride (TCEP) and denaturants like urea, make this enzyme well suited for online intact protein deglycosylation workflows. <a href="javascript:void(0);">(15,16)</a></p><p>In this report, we demonstrate the potential of the PNGase Rc IMER for rapid online deglycosylation of reduced monomeric glycoproteins, including human haptoglobin (Hp), human transferrin, <i>anti</i>-hHD6, and the therapeutic monoclonal antibodies trastuzumab and bevacizumab. The column retained its activity over more than 150 consecutive measurements, highlighting its robustness, suitability for repeated use in bioanalytical workflows, and applicability to pharmaceutical research and development.</p><h4><strong>Experimental Section</strong></h4><h5><strong>Instrumentation and Experimental Conditions</strong></h5><p>Automated sample handling and online deglycosylation were carried out using the HDX workstation (AffiPro, Czech Republic). The scheme of the system is shown on <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig1">Figure 1</a>. Samples of 50 mM ammonium bicarbonate buffer (pH 8.2) and 40 mM TCEP were placed in a sample tray at 4 °C. Ten microliters of the protein sample at a concentration of 0.03 mg/mL (0.3 μg per injection) were added to 40 μL of 50 mM ammonium bicarbonate and then transferred to 50 μL of 40 mM TCEP, pH 2.3. The disulfide bond reduction was carried at 40 °C for 5 min. The automated sample handling system injected 100 μL of the reaction mixture into the sample loop and switched the injection valve. Online deglycosylation was performed at room temperature using a PNGase Rc IMER (2.1 × 20 mm, AffiPro, Czech Republic) at a flow rate of 50 μL/min, followed by protein desalting on an OPTI-TRAP (0.5 × 2 mm) trap column (Optimize Technologies Inc., Oregon City, Oregon, USA) heated to 55 °C for 10 min with 0.2% formic acid delivered by an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1260+Infinity+III+isocratic+pump"><strong>Agilent 1260 Infinity III isocratic pump</strong></a><strong> </strong>(Agilent Technologies, Santa Clara, California, USA). By switching the trap valve, the protein was eluted by an acetonitrile gradient [(min)/% B] 0/10-9/10-16/70-16.5/90-17/90-18/10-23/10] delivered by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+II+LC"><strong>Agilent 1290 Infinity II HPLC</strong></a> system (Agilent Technologies, Santa Clara, California, USA) at a flow rate of 20 μL/min. The mobile phase A consisted of 5% acetonitrile with 1% propan-2-ol and 0.1% formic acid, and the mobile phase B consisted of 95% acetonitrile with 1% propan-2-ol and 0.1% formic acid. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=8&amp;search=maXis+Q-TOF"><strong>maXis Q-TOF mass spectrometer</strong></a> (Bruker Daltonics, Bremen, Germany) was operated in the survey MS mode in a mass range of <i>m</i>/z 50–3000. The acquired data were analyzed and processed by <strong>DataAnalysis 4.4</strong> (Bruker Daltonics) and deconvoluted by UniDec <a href="javascript:void(0);">(17)</a> with the following parameters: data processing range, <i>m</i>/z 500–3000; charge range, 1–80; allowed mass range, 1,000–80,000; sampling rate, 10 Da; peak detection range, 500 Da; and peak detection threshold, 0.1.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_1_Schematic_of_the_automated_sample_handling_system_for_online_protein_deglycosylation_and_desalting_d4ee48b627.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 1. Schematic of the automated sample handling system for online protein deglycosylation and desalting. Protein samples, along with 50 mM ammonium bicarbonate and 40 mM TCEP stock solutions were placed in a sample tray chilled to 4 °C, while a second tray heated to 40 °C was used for disulfide bond reduction with TCEP buffer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_1_Schematic_of_the_automated_sample_handling_system_for_online_protein_deglycosylation_and_desalting_d4ee48b627.jpg 245w," sizes="100vw" width="1287" height="386"></p><h4><strong>Results and Discussion</strong></h4><p>The performance of the PNGase Rc IMER for the removal of complex <i>N</i>-glycans from intact proteins was tested using heavily glycosylated human Hp with four glycosylation sites (Asn184, Asn207, Asn211, and Asn241) <a href="javascript:void(0);">(18)</a> located at the β-chain and occupied by complex <i>N</i>-glycans terminated with sialic acid. Haptoglobin phenotype 2-1, used in this study, forms multimers consisting of β-chain and disulfide linked α1 and α2 chains. <a href="javascript:void(0);">(19)</a> The detection of glycosylated Hp β-chain requires reduction of the disulfide bonds by incubating the protein with a reducing reagent. TCEP is a common reducing reagent used for fast reduction of disulfide bonds at various pH levels. Forty degrees Celsius, the maximum temperature of the PAL tray, and 5 min of incubation were sufficient for complete reduction of Hp disulfide bonds. Individual signals corresponding to the glycosylated β-chain were observed in the <i>m</i>/<i>z</i> range of 35,800–37,400 (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig2">Figure 2</a>a). The limited resolution of the mass spectrometer did not allow assigning the different glycoforms of the haptoglobin β-chain, which is occupied by biantennary, triantennary, or tetraantennary glycans with various amounts of sialic acid and fucose. <a href="javascript:void(0);">(20,21)</a> The nonglycosylated α1 and α2 chains were observed at <i>m</i>/<i>z</i> 9190 and 15,940. When the PNGase Rc IMER was integrated into the flow system, a signal at <i>m</i>/<i>z</i> 27,270 was detected, demonstrating complete deglycosylation of the haptoglobin β-chain (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig2">Figure 2</a>b).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_2_A_Mass_spectrum_of_human_Hp_without_the_PN_Gase_Rc_IMER_implemented_in_the_system_0ecca84ed4.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 2. (A) Mass spectrum of human Hp without the PNGase Rc IMER implemented in the system. The base peak signals of the spectrum correspond to both α1 and α2 chains. The signal of the β chain is spread across its different glycoforms. (B) Mass spectrum of human Hp with the PNGase Rc IMER implemented in the system. The signal of the deglycosylated β chain is higher compared to its glycosylated form and observed only at m/z 27,270. This indicates complete removal of glycans from the Hp β chain." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_2_A_Mass_spectrum_of_human_Hp_without_the_PN_Gase_Rc_IMER_implemented_in_the_system_0ecca84ed4.jpg 245w," sizes="100vw" width="2015" height="913"></p><p>In a previous study by Krenkova et al., the PNGase F IMER was used for deglycosylation of IgG, RNase B, and bovine fetuin with follow-up mass spectrometry detection of both released glycans and intact proteins using MALDI-TOF MS. <a href="javascript:void(0);">(14)</a> The authors demonstrated an effective method for rapid protein deglycosylation, which, however, requires additional sample handling prior to mass spectrometry measurement. The other study by Jmeian et al., which focused on released <i>N</i>-glycans, implemented a PNGase F IMER with online LC–MS detection. <a href="javascript:void(0);">(13)</a> The authors show the effectiveness of the method by identifying many different glycans released from a single protein or from a complex plasma mixture. Our approach is not directly intended for released <i>N</i>-glycan analysis; instead, it demonstrates the ability to measure intact proteins after rapid online deglycosylation using PNGase Rc at low pH. Samples prepared immediately prior to mass spectrometry analysis using the automated sample-handling platform can reduce variability associated with autosampler residence time, as some proteins may undergo degradation or precipitation during storage in the autosampler.</p><p>Although all glycoproteins in this study were effectively deglycosylated, the method’s efficiency depends on the characteristics of the individual glycoprotein. Therefore, optimizing method parameters─including the isocratic flow rate, glycoprotein incubation time with TCEP, and/or the addition of denaturing reagent─may improve the overall efficiency of the deglycosylation process.</p><p>The PNGase Rc IMER stability over a series of injections was tested by using the trastuzumab antibody. The measurements were carried out with the enzyme column kept at the laboratory temperature. As shown in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig5">Figure 5</a>, the results indicate that the column performance remained stable over the course of the 150 measurements, and no glycosylated form of the trastuzumab antibody heavy chain was detected. The activity of PNGase Rc in the presence of 5% acetonitrile and 5% propan-2-ol was evaluated using a bottom-up approach with human 2–1 haptoglobin, which was online-digested by a coimmobilized nepenthesin-2/pepsin IMER. In the absence of an organic solvent, the peptide IEKVVLHPNYSQVD remained fully deglycosylated throughout 30 injections. In contrast, in the presence of organic solvent, a marked loss of enzymatic activity was observed after only a few injections (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.6c00577/suppl_file/ao6c00577_si_001.pdf">Figure 2</a>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_5_Monitoring_the_stability_and_performance_of_PN_Gase_Rc_IMER_4a9b5a9c53.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 5. Monitoring the stability and performance of PNGase Rc IMER. Trastuzumab heavy chain was completely deglycosylated after 150 injections." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_5_Monitoring_the_stability_and_performance_of_PN_Gase_Rc_IMER_4a9b5a9c53.jpg 170w," sizes="100vw" width="996" height="918"></p><h4><strong>Conclusions</strong></h4><p>Here, we demonstrate the potential of the PNGase Rc IMER for the online removal of <i>N</i>-glycans from intact monomeric glycoproteins reduced with TCEP. The column showed high sensitivity toward Hp, which has multiple glycosylation sites with various glycan structures, confirming its suitability for the profiling of complex glycoproteins. Bevacizumab and trastuzumab exhibited similar glycosylation profiles, with core-fucosylated biantennary glycans being the most abundant. Online deglycosylation with the PNGase Rc column resulted in a complete removal of the glycans from the heavy chains of both antibodies, proving that the column can be used for the rapid online deglycosylation of intact therapeutic mAbs. Moreover, the column remained stable over 150 measurements without any loss of activity, even when it was maintained at laboratory temperature. The significantly reduced reaction time, together with full automation and the absence of manual handling steps, substantially improves analytical throughput compared to conventional off-line deglycosylation workflows. These findings highlight the potential of the PNGase Rc IMER in the development of glycoprotein-based biopharmaceuticals, where detailed structural characterization of the protein part is critical for quality control, monitoring batch-to-batch variability, and stability testing.</p></div>

Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor

<p><strong>In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</strong></h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul><p>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.</p><h5><strong>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</strong></h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul><p>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.</p><h5><strong>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</strong></h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul><p>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.</p><h5><strong>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</strong></h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul><p>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.</p><h5><strong>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</strong></h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul><p>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.</p><h5><strong>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</strong></h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul><p>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.</p>

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Waters Otto SPEcialist

Controlled by an interfaced tablet, the easy-to-use Otto software simplifies solid-phase extraction method development and transfer, positive pressure optimization, and real-time manipulation of pressure profiles to further improve method robustness.

<div><div><h4><strong>Consistent, reproducible solid-phase extraction sample preparation &nbsp;</strong></h4></div></div><div><div><p><strong>Improving the reproducibility, method transfer, and processing efficiency of solid-phase extraction (SPE) sample preparation is challenging, and addressing it starts with your solutions. Achieve high-quality sample preparation and extraction by reducing user variability with the programmed pressure profiles and methods on the Otto SPEcialist Positive Pressure Manifold.</strong></p><p>Controlled by an interfaced tablet, the easy-to-use Otto software simplifies <strong>solid-phase extraction</strong> method development and transfer, positive pressure optimization, and real-time manipulation of pressure profiles to further improve method robustness. The <strong>Otto SPEcialist Positive Pressure Manifold</strong> is a semi-automated system designed for use with both SPE plates and cartridges, including µElution and macro elution plates and 1 cc, 3 cc, and 6 cc cartridges.</p><div><div><div><div><ul><li><strong>Improve reproducibility</strong> and method development for SPE sample preparation</li><li><strong>Achieve consistent performance</strong> across SPE runs by eliminating technique variation due to differing levels of user expertise</li><li><strong>Accommodate varying SPE processing needs</strong> from very low to high flow rates for sorbent bed drying</li><li>Ensure novice and expert SPE users can get up and running quickly with <strong>easy-to-use software</strong> that requires little to no training</li><li><strong>Perform multiple workflows</strong> in a single day</li><li>Process a wide range of sample volumes using <strong>micro and macro elution plates</strong>; and <strong>1 cc, 3 cc, and 6 cc cartridges</strong></li><li><strong>Compact device</strong> that fits easily on a bench or under a hood</li></ul></div></div><div><div><h5><strong>Recommended Use</strong></h5><p>To improve reproducibility and analytical consistency during <strong>sample preparation and solid-phase extraction</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Otto_SP_Ecialist_4_66c2f095a0.jpg" alt="Waters Otto SPEcialist: The Otto Specialist Positive Pressure Manifold is designed to improve analytical consistency during sample preparation and solid-phase extraction. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Otto_SP_Ecialist_4_66c2f095a0.jpg 234w," sizes="100vw" width="1024" height="682"></p><div><div><h3><strong>Optimize solid-phase extraction workflow efficiency</strong></h3><p>The <strong>Otto SPEcialist Positive Pressure Manifold</strong> is controlled by the easy-to-use <strong>Otto Software</strong>. The <strong>touchscreen interface</strong> requires little-to-no training for novice and expert SPE users. Easily create pressure profiles with optimized flow rates for each step of your process. Whether it’s a simple “load, wash, elute” or a more complicated workflow with multiple steps, <strong>Otto Software</strong> makes running programed methods simple.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Otto_SP_Ecialist_ade08ca842.jpg" alt="Waters Otto SPEcialist: Pressure profile example created in the Otto SPEcialist software designed to increase pressure over time. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Otto_SP_Ecialist_ade08ca842.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div><div><div><h3><strong>Ensure consistent SPE</strong></h3><p>Running programmed methods, including a combination of pressure profiles and manifold commands, eliminates user-to-user variability ensuring consistency across users and labs. <strong>Otto Software</strong> tracks and documents each pressure profile and method processed, ensuring workflow consistency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Otto_SP_Ecialist_2_9dc31e4ffc.jpg" alt="Waters Otto SPEcialist: Method set-up on an Otto SPEcialist System incorporating opening and closing of the manifold, pausing the method, and running a pressure profile. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Otto_SP_Ecialist_2_9dc31e4ffc.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div><div><div><h3><strong>Reproducible, high-quality results</strong></h3><p><strong>Reproducibility</strong> is especially important when generating <strong>calibration curves</strong>. With its <strong>programmable pressure profiles</strong> and methods, <strong>Otto SPEcialist Positive Pressure Manifold</strong> performs consistently across all SPE runs, independent of user expertise.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Otto_SP_Ecialist_3_adeb0ee489.jpg" alt="Waters Otto SPEcialist: Calibration curves for sulfadimethoxine generated on a vacuum manifold and Otto SPEcialist by a novice user. Replicates (n=3) of each level of the curve were run and standard deviations were determined and are indicated error bars. The Otto SPEcialist error bars, while present are very small and difficult to discern. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Otto_SP_Ecialist_3_adeb0ee489.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div></div></div></div></div></div></div>

Waters SYNAPT XS High Resolution Mass Spectrometer

The SYNAPT XS Ion Mobility Time-of-Flight Mass Spectrometer provides ultimate flexibility, offering greater freedom of analytical choice to support scientific creativity and technical success for any application.

<div><div><h4><strong>Ultimate flexibility for deeper discovery</strong></h4></div></div><div><div><p><strong>In the arena of scientific discovery, the slow pace of advancement and escalating costs of research can prove to be a challenge. Unlike competitor systems with restricted inlet options, scan function limitations, or requiring multiple platforms, only Waters offers an all-encompassing high-performing LC-MS solution, the SYNAPT XS mass spectrometer, for ultimate flexibility and greater freedom of analytical choice – supporting scientific creativity and technical success for any application.</strong></p><ul><li><strong>Novel technology building blocks deliver cutting-edge analytical performance</strong></li><li><strong>SONAR</strong> and <strong>HDMSE</strong> provide a unique toolkit for interrogating complex mixtures</li><li><strong>Ion mobility</strong> multiplies peak capacity and selectivity of analysis</li><li><strong>CCS measurements</strong> increase security of compound identification</li><li><strong>Deeper discovery in Mass Spectrometry Imaging</strong> with<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/Full-Spectrum-Molecular-Imaging-/nav.htm?cid=135070661"><strong>Full Spectrum Molecular Imaging</strong></a> enabled by <a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/DESI-XS---Desorption-Electrospray-Ionization-/nav.htm?cid=135047466"><strong>DESI XS</strong></a> and MALDI</li></ul><h4><strong>Overview</strong></h4><div><div><div><div><ul><li><strong>Serve the broadest range of applications </strong>with the most extensive range of chromatograph inlets for ultimate flexibility</li><li><strong>Attain fragment ion information for every detectable component</strong> for unambiguous confirmation of compound identity with DIA modes <strong>SONAR</strong>, MSE, and <strong>HDMSE</strong></li><li><strong>Generate rich fragmentation data</strong> from highly complex samples in all acquisition modes including DDA</li><li>Simplify and streamline the mass spectral imaging workflow and deliver multi-layered, information-rich data with multiple imaging techniques and accompanying High Definition Imaging (HDI) Software</li><li><strong>Achieve automated higher order structure determination </strong>thorough confident identification, intact mass analysis, and best-in-class reproducibility</li><li><strong>Enhance MS/MS possibilities</strong> using complementary fragmentation techniques with high resolution and accurate mass measurements</li></ul></div></div><div><div><h5><strong>Recommended Use</strong></h5><p>For deeper discovery by aligning complimentary workflows on a single <strong>Ion mobility</strong> enabled time-of-flight mass spectrometer with the flexibility of multiple ion sources and acquisition modes.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_4_696d77ce2b.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: The Waters SYNAPT XS offers flexibility and freedom of analytical choice with an extensive range of chromatographic inlets." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_4_696d77ce2b.jpg 234w," sizes="100vw" width="1024" height="682"></p><div><div><h3><strong>Cutting edge performance with novel technology</strong></h3><p>With technology building blocks inherited from the <strong>Waters Advanced Mass Spectrometry</strong> <strong>SELECT SERIES</strong>, advanced innovation is built-in, ensuring scientists using this platform are at the cutting edge of mass spectrometry while retaining the ease of use and established end-to-end workflows for which the <strong>SYNAPT </strong>is well known.</p><ul><li><strong>StepWave&nbsp;XS -&nbsp;</strong>Redesigned segmented quadrupole transfer optics provide enhanced sensitivity for challenging compounds while further improving levels of analytical robustness.</li><li><strong>Extended ToF -&nbsp;</strong>Delivers UPLC-compatible mass resolution, matrix-tolerant dynamic range, and quantitative results for the most complex samples, while offering superior specifications to previous SYNAPT models.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_9f19abb382.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: SYNAPT XS schematic." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_9f19abb382.jpg 245w," sizes="100vw" width="1024" height="546"></p></div></div><div><div><h3><strong>Interrogate complex mixtures with a unique toolkit</strong></h3><p>A complete analytical strategy requires the combination of appropriate complementary techniques to see the bigger picture. With both <strong>SONAR</strong> and IMS-based <strong>data independent acquisition</strong> (DIA) modes of operation on the SYNAPT XS, the discovery analyst is in a unique position to interrogate complex mixtures by complementary mechanisms. Both types of acquisition increase analytical <strong>peak capacity</strong>, providing ‘clean and clear’ fragmentation data, but based on different molecular properties. This provides a truly unique investigative toolbox for the in-depth interrogation of complex mixtures.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_2_9179e575a4.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: During each scan the quadrupole separates co-eluting precursor masses by scanning the mass range, and transiting them in sequence. Fragment ions from different precursors can then be recorded separately and assigned with confidence." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_2_9179e575a4.jpg 245w," sizes="100vw" width="1024" height="593"></p></div></div><div><div><h3><strong>Discover more with ion mobility and CSS measurement</strong></h3><p>Conventional mass spectrometers separate components based on m/z. The SYNAPT XS also allows separation of molecules according to<strong> their size, shape, and charge during ion mobility experiments as a function of their collisional cross section</strong> (CCS). As well as ion mobility offering an additional dimension of resolution, multiplying <strong>peak capacity</strong>, and <strong>selectivity of analysis</strong> the measurement of CCS offers additional molecular identifiers. The measured CCS of an ion can be used to help confirm an ion’s identity or investigate its structure. The inclusion of ion mobility significantly increases the extent and confidence with which a scientist can profile complex mixtures and complex molecules – dramatically enhancing sample definition.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_3_2240bff527.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: Retention and drift time principle ion mobility enabled data-independent LC-HDMSE analysis (LC-DIA-IM-MS)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_3_2240bff527.jpg 204w," sizes="100vw" width="1024" height="784"></p></div></div><div><div><h3><strong>Achieve ultra-confident structural characterization of components</strong></h3><p>Time aligned parallel (TAP) fragmentation is an acquisition mode unique to the T-Wave IMS design. It permits users to take the advantage of the <strong>TriWave configuration</strong>, which allows the pre-IMS T-Wave and post-IMS T-Wave to operate as two separate collision cells. The resultant CID-IMS-CID instrumental operation helps to provide ultra-confident structural characterization of components. <strong>TAP fragmentation</strong> provides a distinct advantage for building a complete structure, through superior fragment ion coverage, sensitivity, and accuracy compared to traditional MSⁿ or MS/MS techniques.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_5_4dd33f1ab5.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: Schematic showing Time Aligned Parallel Fragmentation. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_5_4dd33f1ab5.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div></div></div></div></div></div></div>

Waters BioAccord LC-MS System

The BioAccord LC-MS System enables routine biotherapeutic monitoring across development, manufacturing, and QC, with a compact design and user-friendly platform for non-MS experts.

<div><div id="title-61fa75209f" data-locator="title-with-text"><h3><strong>Routine biopharmaceutical analysis – accessible and accelerated</strong></h3></div></div><div><div id="text-73a19e9720" data-locator="text"><p><strong>Improving lab efficiency without sacrificing result quality is important but challenging. Now you can achieve high-quality biotherapeutic molecule data while maximizing efficiency with the ability to directly measure product attributes to assess product quality and process robustness.</strong></p><p><strong>The BioAccord LC-MS System</strong> is specifically designed for routine definition and monitoring of biotherapeutic attributes across biopharmaceutical process and product development, manufacturing, and QC, all within a small system footprint on a platform capable of operation by non-MS experts.&nbsp;</p><p>Analyze a broad range of product attributes using the intact molecule, subunits, peptide maps, and released glycans, all with greater sensitivity, selectivity, and dynamic range than traditional chromatographic and electrophoretic techniques.</p><ul><li>Make rapid, confident decisions with accessible, consistent high-quality data​</li><li>Ensure operational simplicity, result consistency, and lab productivity with workflow automation​</li><li>Compliant-ready platform that can be deployed and replicated from discovery through to QC.​</li><li>Achieve fast deployment, reliable operation, and a clear return on investment​</li></ul><h3><strong>Overview</strong></h3><ul><li>Compliant-ready platform ideal to deploy from discovery to quality control (QC)​</li><li>Achieve faster time to value, and consistent performance through automated set-up with SmartMS</li><li>Diagnose, and fix potential system issues before they affect data quality with intelligent health checks in SmartMS</li><li>Focus your team on what matters with a system that doesn't require LC-MS experts for routine operation</li><li>Make more informed, data-driven decisions with trustworthy LC-MS data directly available at the point of decision-making</li><li>Achieve the highest quality data in your lab, comparable to data previously available only from the complicated, and costly LC-MS systems used for product characterization</li></ul><p><strong>Recommended Use: </strong>For the rapid measurement of multiple product quality attributes during routine biopharmaceutical analysis.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_R_Da_Detector_1_c570b28cc2/Waters-ACQUITY-RDa-Detector-1.jpeg" alt="Waters BioAccord LC-MS System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_R_Da_Detector_1_c570b28cc2/thumbnail_Waters-ACQUITY-RDa-Detector-1.jpeg 125w," sizes="100vw" width="1024" height="1274"></p><h3><strong>Accelerate decisions with less effort to collect and process high quality data</strong></h3><p>Minimize the time spent generating data and managing LC-MS performance and maximize time for actions driven by the high quality data generated with automated set-up and guided workflows.&nbsp;</p><h4><strong>Use the streamlined workflows with the BioAccord LC-MS System for:</strong></h4><ul><li>Intact mass analysis (proteins, subunits, oligonucleotides, mRNA fragments)</li><li>Peptide mapping and multi-attribute method (MAM)</li><li>Released N-glycan analysis</li><li>Oligonucleotide sequence confirmation</li><li>Impurity analysis for proteins, peptides, and oligonucleotides</li></ul><p>These workflows streamline method set-up, data acquisition and processing, and report generation to deliver the required information with minimal manual interventions.​ In addition to these core workflows, the BioAccord System also supports a range of other applications required in biotherapeutic process and product development, like cell culture media analysis, mRNA cap, and lipid nanoparticle analysis.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Bio_Accord_LC_MS_System_Visualize_your_data_with_intuitive_and_easy_to_review_layouts_9876dbe5a8.jpg" alt="Waters BioAccord LC-MS System: Visualize your data with intuitive and easy to review layouts. These results from a peptide MAM analysis show product quality attributes that are being monitored, flagging those that are out of specification." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Bio_Accord_LC_MS_System_Visualize_your_data_with_intuitive_and_easy_to_review_layouts_9876dbe5a8.jpg 245w," sizes="100vw" width="954" height="541"></p><h3><strong>Increase productivity by increasing data quality and reducing analysis failures</strong></h3><p>Non-specific adsorption of metal sensitive analytes can be unpredictable and challenging, leading to long system passivation times, large relative standard deviations (RSDs), and broad peaks that can be tough to detect and quantify.</p><p>The BioAccord LC-MS System incorporates ACQUITY Premier UPLC and Premier chemistries with MaxPeak High Performance Surfaces (HPS) Technology. These innovations maximize system readiness, decrease time to operation, and enable superior run-run and system-system consistency while improving sensitivity for the metal interacting analytes.&nbsp;</p><h4><strong>Through the application of MaxPeak HPS Technology, you can:</strong></h4><ul><li>Increase recovery and consistent detection of problematic peptides for enhanced peptide map and MAM analysis.</li><li>Achieve superior sensitivity and reproducibility for acidic glycans and lipids by minimizing analyte/surface interactions that lead to sample loss.</li><li>Save time and improve productivity by reducing or eliminating passivation for robust oligonucleotide analyses.</li><li>Minimize the risk of undetected peaks by minimizing metal-sensitive analyte loss, improving recovery and the lowering limits of detection.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Bio_Accord_LC_MS_System_The_figure_A_shows_the_extracted_ion_chromatograms_XIC_for_GFYPSDIAVEWESNGQPENNYK_PENNYK_peptide_unmodified_and_deamidated_peptides_collected_for_a_NIS_Tm_Ab_digest_b14145461f.jpg" alt="Waters BioAccord LC-MS System: The figure (A) shows the extracted ion chromatograms (XIC) for GFYPSDIAVEWESNGQPENNYK (PENNYK) peptide unmodified and deamidated peptides collected for a NISTmAb digest. The chromatographic trace in solid line shows the XIC for PENNY peptides acquired on the BioAccord System. The inset shows all modification of PENNY peptide including succinimide modification. Figure (B) shows the total normalized peak area calculated for PENNY peptides using conventional and ACQUITY Premier UPLC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Bio_Accord_LC_MS_System_The_figure_A_shows_the_extracted_ion_chromatograms_XIC_for_GFYPSDIAVEWESNGQPENNYK_PENNYK_peptide_unmodified_and_deamidated_peptides_collected_for_a_NIS_Tm_Ab_digest_b14145461f.jpg 245w," sizes="100vw" width="1024" height="512"></p><h3><strong>Obtain quality data, faster than ever before</strong></h3><p>Quickly getting the quality data you need for your laboratory to stay productive is paramount but deploying traditional LC-MS systems is often rather slow. The integrated nature of the BioAccord LC-MS System means the system can be deployed easily and users can be productive faster through the delivery of system and applications training with appropriate biopharmaceutical methods, sample data, and system check standards for the common biopharmaceutical analysis workflows.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Bio_Accord_LC_MS_System_Mass_accuracy_summary_for_48_injections_that_representing_six_antibodies_with_eight_replicate_injections_each_ed012d8ad7.jpg" alt="Waters BioAccord LC-MS System: Mass accuracy summary for 48 injections that representing six antibodies with eight replicate injections each. Mass accuracy summary for 48 injections that representing six antibodies with eight replicate injections each." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Bio_Accord_LC_MS_System_Mass_accuracy_summary_for_48_injections_that_representing_six_antibodies_with_eight_replicate_injections_each_ed012d8ad7.jpg 245w," sizes="100vw" width="855" height="206"></p><h4><strong>With the BioAccord LC-MS System, you can:</strong></h4><ul><li>Ensure optimal system performance with biomolecule-based installation testing.</li><li>Enable users to quickly get up to speed with outcome-based application training.</li><li>Achieve high quality data with existing personnel or respond quickly to staff and project turnover challenges with reduced training burdens.</li></ul><h3><strong>Meeting your regulatory requirements, together</strong></h3><p>Meeting corporate and regulatory expectations for compliance and data integrity is critical to your laboratory operations, and those needs don’t change because LC-MS technology is moving into roles within manufacturing and quality control (QC) organizations.</p><p>Your data quality and compliance are our priority, too. The BioAccord System is built on the compliant-ready waters_connect informatics platform and backed by the long history at Waters of delivering compliant informatics and professional qualification services to streamline deployment.</p><h4><strong>With Waters, you’ll get:</strong></h4><ul><li>A single informatics platform based on a secure relational database architecture</li><li>Full audit trail for acquisition, processing, and reporting of data</li><li>Administrator-configurable roles and permissions</li><li>Network deployment of systems for maximum data safety and controlled accessibility</li><li>Characterization (Q-Tof) and routine analysis (BioAccord) systems on a common network for efficient system, data, and information management</li><li>Professional qualification services</li></ul></div></div>

Waters RADIAN ASAP Direct Mass Detector

Combining flexibility, ease of operation, and speed without the need for complex sample preparation, separation or extensive MS experience, the RADIAN ASAP helps you and your laboratory achieve more.

<div><div id="title-a9e634f6cb" data-locator="title-with-text"><h3><strong>The power of knowing now</strong></h3></div></div><div><div id="text-c687cac6aa" data-locator="text"><p><strong>Whether you are looking to improve your drug discovery cycle, expand your client services, or simply become more competitive in your turnaround times, integrating direct mass spectrometry (MS) analysis can drive multiple system-wide efficiencies helping to achieve your operational goals.</strong></p><p>Combining flexibility, ease of operation, and speed without the need for complex sample preparation, separation or extensive MS experience, the RADIAN ASAP helps you and your laboratory achieve more.</p><ul><li>Simple operation and data interpretation</li><li>Minimal sample preparation</li><li>No separation required</li><li>Real-time results and classification</li></ul><figure class="image"><img style="aspect-ratio:454/321;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_RADIAN_ASAP_7cfe24b23d/Waters-RADIAN-ASAP_s.webp" alt="Waters RADIAN ASAP Direct Mass Detector" width="454" height="321"></figure><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-6b896f6da4" data-locator="text"><ul><li>Ensure users get up and running quickly with RADIAN ASAP’s easy to learn and fully automated set up, streamlined workflow, and minimal training requirements</li><li>Minimize sample prep with direct sampling onto glass probe</li><li>Minimize the need for solvent use and disposal with no chromatography</li><li>Save time with 30 second analysis and real-time sample classification</li></ul></div></div><div><div id="text-161a2a5c01" data-locator="text"><h4><strong>Recommended Use</strong></h4><p>For rapid, easy, and low-cost analysis of liquids and solids.</p><div><div id="text-4973311325" data-locator="text"><h3 data-locator="modal-title"><strong>Compact without compromise</strong></h3><p>The RADIAN ASAP is a novel, dedicated direct analysis system specifically designed for rapid, easy and low-cost analysis of liquids and solids. Engineered using proven and robust single quadrupole mass spectrometry technology combined with the Atmospheric Pressure Solids Analysis Probe (<a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/services/instrument-services/instrument-upgrades/mass-spectrometry-ms-upgrades/atmospheric-solids-analysis-probe.html" data-di-id="di-id-70d78c77-7e033f9d">ASAP</a>), the RADIAN ASAP delivers quality and ease of use in a compact footprint.&nbsp;</p><p>This single quadrupole mass detector provides high quality mass spectral data, without the need for any special training or expertise, eliminating any need to contract out and wait for results from specialist analytical service laboratories.</p></div></div><div><div id="text-08366f2c0d" data-locator="text"><h3 data-locator="modal-title"><strong>Flexible workflow solutions in 4 simple steps</strong></h3><p>Operation of the RADIAN ASAP is simple and intuitive, allowing users to generate results with minimal or no sample preparation, making it an ideal tool for laboratories to make informed decisions. Capable of ionizing molecules directly from a liquid or solid makes the system available to a wide range of applications and workflows.</p><h4><strong>Generate results in 4 simple steps:</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_RADIAN_ASAP_analyza_ve_4_jednoduchych_krocich_a997bfddda/Waters-RADIAN-ASAP-analyza-ve-4-jednoduchych-krocich.jpg" alt="Waters RADIAN ASAP Direct Mass Detector: Flexible workflow solutions in 4 simple steps" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_RADIAN_ASAP_analyza_ve_4_jednoduchych_krocich_a997bfddda/thumbnail_Waters-RADIAN-ASAP-analyza-ve-4-jednoduchych-krocich.jpg 245w," sizes="100vw" width="1683" height="490"></p><h3><strong>Ideal for numerous application requirements</strong></h3><p><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/nav.htm?cid=135078611" data-di-id="di-id-7f758d90-ccee23de"><strong>Rapid triage of seized drug materials for Forensics</strong></a><i>&nbsp;<strong>—</strong>&nbsp;</i>RADIAN ASAP and LiveID ensure crime labs can quickly screen samples against a library of known compounds for rapid, confident identification of illicit drugs. The sensitivity and selectivity of mass spectrometry provides confidence in detecting drugs with an easy, efficient triage workflow.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/nav.htm?cid=134528742" data-di-id="di-id-3a3c46e4-ccee23de"><strong>Assess food integrity for Food &amp; Environmental</strong></a>&nbsp;<strong>—</strong> The threat posed by food fraud has created additional demands on both the food industry and regulators to protect food consumers and maintain brand reputation. RADIAN ASAP and LiveID offer a powerful combination of mass spectrometry and chemometric modeling, allowing users to easily and efficiently screen samples and make quicker decisions.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.tainstruments.com/radian-asap-direct-mass-detector/" data-di-id="di-id-2fac456-44144a9d"><strong>Fast chemical analysis for improved formulation testing in Chemical and Materials</strong></a><i>&nbsp;<strong>—</strong>&nbsp;</i>RADIAN ASAP and IonLynx supports quality control (QC) and development labs in the chemical industry to reduce costs and increase efficiencies. Streamline workflows like fast chemical analysis for improved formulation testing or&nbsp;material release testing. Features including thermal gradient analysis are ideal for the complex samples in the chemical industry.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/nav.htm?cid=134751395" data-di-id="di-id-76921eaa-ccee23de"><strong>Discovery reaction monitoring&nbsp;for Pharma</strong></a>&nbsp;<strong>—</strong> The demand to accelerate the drug discovery and development process has never been higher.&nbsp;RADIAN ASAP provides synthetic chemists easy access to mass spectral data for near-instantaneous assessment of reaction progress and identification of purification fractions, increasing lab efficiency.</p><div><div><div><div id="text-8b0fa56c8c" data-locator="text"><p><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/nav.htm?cid=134971387" data-di-id="di-id-10e51f17-ccee23de"><strong>Undergraduate teaching&nbsp;and method development&nbsp;in Academia</strong></a><strong> —&nbsp;</strong>Simplicity and ease of use makes RADIAN ASAP the ideal tool for busy academic laboratories that need a robust and reliable solution.</p><figure class="image"><img style="aspect-ratio:474/308;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_RADIAN_ASAP_2_87ddc46f15/Waters-RADIAN-ASAP-2_s.webp" alt="Waters RADIAN ASAP Direct Mass Detector" width="474" height="308"></figure><div><div id="text-a5e5e26910" data-locator="text"><h3 data-locator="modal-title"><strong>Software solutions for your data requirements</strong></h3><p><strong>RADIAN ASAP</strong> is compatible with software solutions for rapid interpretation and classification as well as automated data processing and reporting.</p><p>For customers investigating the authenticity, adulteration, and quality of food or raw materials, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=liveid"><strong>LiveID</strong></a> uses statistical models to classify samples. First, authentic or verified samples are used to create and validate a statistical model. The validated model can then be used with test samples and generate live classifications with a simple yes/no answer output. LiveID can also do real time <strong>library</strong> matching workflows, where the software matches all compounds in the library against each sample analyzed, giving a score out of <strong>1000</strong>, where values close to 1000 indicate the compound of interest is likely in the sample and lower scores indicate unlikely presence in the sample.</p><p><strong>OpenLynx</strong> processing provides user customizable processing capabilities to generate automated mass confirmation reports in either pdf, printed or browser format.&nbsp;</p><p><strong>IonLynx</strong> allows users to determine levels and relative ratios of analytes of interest in complex samples to aid decision making in QC and formulation monitoring. IonLynx provides automated processing and reporting of non-chromatographic data sets, such as those generated by RADIAN ASAP.</p></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

Waters SARS-CoV-2 LC-MS Kit (RUO)

Advance virus research with Waters' RUO LC-MS kit for SARS-CoV-2. Detect and quantify NCAP peptides with high sensitivity and reproducibility using SISCAPA antibody enrichment and automation-ready workflows.

<h4><strong>Detect and quantify SARS-CoV-2 NCAP peptides to advance virus research</strong></h4><p>The global <strong>COVID-19 pandemic</strong> created an unprecedented need to quickly deploy emergency tests for SARS-CoV-2 infection. Now, scientists can harness the flexibility and reproducibility of LC-MS to advance critical SARS-CoV-2 research.</p><p><strong>Waters SARS-CoV-2 LC-MS Kit (RUO)</strong> is a <strong>Research Use Only kit</strong> to directly <strong>detect and quantify SARS-CoV-2</strong> signature nucleocapsid peptides (NCAPs) without the need to amplify the target analytes – so you can ensure accuracy, reproducibility, and sensitivity for your most critical research.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SARS_Co_V_2_LC_MS_Kit_RUO_2_50b4ae9b00/Waters-SARS-CoV-2-LC-MS-Kit-(RUO)-2.jpg" alt="Waters: SARS-CoV-2 LC-MS Kit (RUO)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SARS_Co_V_2_LC_MS_Kit_RUO_2_50b4ae9b00/thumbnail_Waters-SARS-CoV-2-LC-MS-Kit-(RUO)-2.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Overview</strong></h4><ul><li>Achieve <strong>reproducible results</strong> between and within labs &nbsp;</li><li>Ensure <strong>high accuracy</strong> and analytical <strong>sensitivity</strong> to enable critical research &nbsp;</li><li><strong>Empower research</strong> and development of pioneering SARS-CoV-2 testing with an alternative and complementary technology to common infectious pathogen research &nbsp;</li><li>Enable <strong>broader research with an LC-MS method </strong>with the potential to be multiplexed with other peptides and biomarkers&nbsp;</li></ul><p><i><strong>* </strong>This kit is for research use only and has not been approved for use in clinical diagnostic procedures. This RUO kit has not been tested with clinical samples. The SARS-CoV-2 kit for SARS-CoV-2 nucleocapsid peptides is not available in all countries, please contact your Waters representative for more details.</i></p><p><strong>Recommended Use:</strong> For the detection and quantification of SARS-CoV-2 signature NCAP peptides.</p><h4><strong>LC-MS enables cutting-edge SARS-CoV-2 research</strong></h4><p>The Waters SARS-CoV-2 LC-MS Kit (RUO) is a Research Use Only kit intended for the quantitative measurement of three proteolytic peptides derived from the SARS-CoV-2 Nucleocapsid (NCAP) protein. The method has been optimized on an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/199"><strong>ACQUITY UPLC I-Class PLUS</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/195"><strong>Xevo TQ-XS System</strong></a> and is also intended for use with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/208"><strong>Andrew+ Pipetting Robot</strong></a>. Optimization on other instrument platforms is possible. &nbsp;</p><p>Further investigation is needed to collect objective evidence in promising research applications, however, based on the properties of the analytical test method, the SARS-CoV-2 LC-MS Kit might be leveraged for the following research studies: &nbsp;</p><ul><li>Peptide concentration to quantitate SARS CoV-2 signature peptides at different stages of infection&nbsp;</li><li>Research of infection and long-term effects through peptide quantitation&nbsp;</li></ul><p>Future pandemic preparedness studies and research of signature peptide-based biomarkers unique to other infections </p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_The_SARS_Co_V_2_LC_MS_Kit_RUO_allows_for_easy_sample_preparation_sample_enrichment_and_sample_detection_f0aca034ad.jpg" alt="Waters: The SARS-CoV-2 LC-MS Kit (RUO) allows for easy sample preparation, sample enrichment, and sample detection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_The_SARS_Co_V_2_LC_MS_Kit_RUO_allows_for_easy_sample_preparation_sample_enrichment_and_sample_detection_f0aca034ad.jpg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Achieve confidence in results</strong></h4><p>Ensure accurate and consistent results with highly analytically selective antibody magnetic bead enrichment. With the innovative SISCAPA® anti-peptide antibody enrichment sample preparation method from SISCAPA Assay Technologies, Inc. in the SARS-CoV-2 LC-MS Kit (RUO), you can improve the performance of mass spectrometry for measurement of pre-selected protein targets.</p><p>With the SARS-CoV-2 LC-MS Kit (RUO) and SISCAPA, you can achieve the analytical sensitivity you need to detect low levels of SARS-CoV-2 peptides from complex digested samples with stable isotope standards and capture by anti-peptide antibodies. Increase laboratory efficiency with all the components your busy research laboratory needs to quickly deploy the supplied method - all in one package.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_The_SARS_Co_V_2_method_minimizes_sample_media_interference_providing_greater_flexibility_for_the_analysis_of_multiple_research_samples_fe3a56c686.jpg" alt="Waters: The SARS-CoV-2 method minimizes sample media interference, providing greater flexibility for the analysis of multiple research samples." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_The_SARS_Co_V_2_method_minimizes_sample_media_interference_providing_greater_flexibility_for_the_analysis_of_multiple_research_samples_fe3a56c686.jpg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Ensure reproducibility with trusted chemistries and consumables</strong></h4><p>Ensuring reproducible quantitation from sample-to-sample, run-to-run, and lab-to-lab provides the confidence you need for critical research decisions. Ensuring reproducibility starts with minimizing variability risks, including the risk of unpredictable non-specific binding of peptides to surfaces. The SARS-CoV-2 LC-MS Kit uses QuanRecovery Plates with MaxPeak HPS and a MaxPeak Premier Peptide Column, minimizing non-specific binding of peptides to surfaces while enhancing recovery and signal.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analytical_sensitivity_of_the_ADE_AYN_and_NPA_peptides_in_VTM_at_2_3_5_10_and_20_amol_L_following_antibody_enrichment_145d5249ef.jpg" alt="Waters: Analytical sensitivity of the ADE, AYN, and NPA peptides in VTM at 2, 3, 5, 10, and 20 amolµL following antibody enrichment." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analytical_sensitivity_of_the_ADE_AYN_and_NPA_peptides_in_VTM_at_2_3_5_10_and_20_amol_L_following_antibody_enrichment_145d5249ef.jpg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Let Andrew+ pipette for you</strong></h4><p>Save valuable time and focus on your research with reliable and reproducible sample preparation using the Andrew+ Pipetting Robot. The SARS-CoV-2 LC-MS Kit (RUO) comes in an automation-friendly format with liquid handling protocols for the Andrew+ Pipetting Robot available on OneLab.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SARS_Co_V_LC_MS_Kit_RUO_automation_protocol_available_for_Andrew_Pipetting_Robot_It_provides_a_flexible_automation_platform_easily_adaptable_to_multiple_workflows_8dca9a99e0.jpg" alt="Waters: SARS-CoV-LC-MS Kit (RUO) automation protocol available for Andrew + Pipetting Robot. It provides a flexible automation platform easily adaptable to multiple workflows." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SARS_Co_V_LC_MS_Kit_RUO_automation_protocol_available_for_Andrew_Pipetting_Robot_It_provides_a_flexible_automation_platform_easily_adaptable_to_multiple_workflows_8dca9a99e0.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters SELECT SERIES MRT mass spectrometer

Accelerate your research and comprehensively identify analytes in your complex samples with the SELECT SERIES MRT, built with state-of-the-art Multi Reflecting Time-of-Flight (MRT) technology. 

<div><div id="title-089dfa8549" data-locator="title-with-text"><h3><strong>Redefine performance with all the benefits of time-of-flight technology</strong></h3></div></div><div><div id="text-77997605f8" data-locator="text"><p><strong>Accelerate your research and comprehensively identify analytes in your complex samples with the SELECT SERIES MRT, built with state-of-the-art Multi Reflecting Time-of-Flight (MRT) technology. The SELECT SERIES MRT provides a unique combination of ultra-high resolution and specificity independent of scan speed, resulting in unambiguous compound identification with even the most complex samples.</strong></p><p>Distinguish the fine isotope structure of small molecules, resolve nominally isobaric interferences, and baseline resolve the isotopic envelope of multiply-charged ions with high resolution MRT technology. Unlike other high-resolution mass analyzers, SELECT SERIES MRT requires no compromise to your experiments, since mass resolution is consistent over a broad mass range and scan speeds.</p><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-b57ec45d49" data-locator="text"><ul><li>Confidently identify sample components with routine ppb mass accuracy&nbsp;</li><li>Resolve nominally isobaric interferences with high mass resolution, at all scan speeds</li><li>Reveal isotopic fine structure for unambiguous elemental compositions</li><li>Achieve high data quality in both MS and MS/MS</li><li>Simplify data archiving and rapidly process data with reduced data files sizes</li><li>Seamlessly interchange between MALDI, DESI XS and Electrospray sources for superior accuracy for compound identification</li></ul></div></div><div><div id="text-590eba5dee" data-locator="text"><h3><strong>Recommended Use</strong></h3><p>For unambiguous compound identification of even the most complex samples.</p><div><div id="text-d3d9e01296" data-locator="text"><h3 data-locator="modal-title"><strong>High resolution mass spectrometry, without compromise</strong></h3><p>The SELECT SERIES MRT achieves ground-breaking Time-of-Flight (ToF) resolution with the innovative use of multiple reflections of the ion beam between two gridless electrostatic mirrors in the mass analyzer. This extends the ions’ flight&nbsp;path, spatially refocusing the ion packets through a series of periodic lenses after each reflection maintaining sensitivity.&nbsp;Resolution Enhancement Mode (REM) extends the flight path by deflecting ions back for a second pass of the MRT analyzer increasing the resolving power by &gt;50% enabling &gt;300,000 FWHM.</p><figure class="image"><img style="aspect-ratio:1272/897;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Schema_konstrukce_SELECT_SERIES_MRT_Q_Tof_systemu_a07ed0a724/Waters-Schema-konstrukce-SELECT-SERIES-MRT-QTof-systemu.jpg" alt="Waters SELECT SERIES MRT: instrument schematic." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Schema_konstrukce_SELECT_SERIES_MRT_Q_Tof_systemu_a07ed0a724/thumbnail_Waters-Schema-konstrukce-SELECT-SERIES-MRT-QTof-systemu.jpg 221w," sizes="100vw" width="1272" height="897"></figure><p>The superior performance of the SELECT SERIES MRT results in high precision and routine part-per-billion mass accuracy independent of acquisition speed, providing unsurpassed quality of mass spectrometry data and information. The instrument is operated via the SELECT SERIES&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/nav.htm?cid=513164" data-di-id="di-id-e7513c98-b105e917">MassLynx</a>&nbsp;acquisition system with simple and intuitive experimental setup, acquisition, and processing of mass spectrometry data. It records data in the widely deployed Waters .raw file format, which you can easily access via the MassLynx API, export as (i)mzML, and other interchangeable data formats, ready to use in your data analysis strategy.</p><figure class="image"><img style="aspect-ratio:1024/682;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_MRT_REM_mode_fine_isotope_structure_for_caffeine_1443227594.jpg" alt="Waters SELECT SERIES MRT: REM mode fine isotope structure for caffeine." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_MRT_REM_mode_fine_isotope_structure_for_caffeine_1443227594.jpg 234w," sizes="100vw" width="1024" height="682"></figure><div><div id="text-78cca88bb7" data-locator="text"><h3 data-locator="modal-title"><strong>Redefining confidence for LC-MS</strong></h3><p>The SELECT SERIES MRT provides a new level of confidence for LC/MS experiments by routinely obtaining ppb mass accuracy, revealing fine isotope structure, all with the exceptional usability of a state-of-the-art Quadrupole Time-of-Flight (QToF). The spectacular performance enables search tolerances to be minimized, providing higher confidence in elemental composition and molecular formula assignments for known and unknown targets. Moreover, the ability to routinely access such high specificity from accurate mass for both precursor and product ions is truly transformative.</p><figure class="image"><img style="aspect-ratio:1024/570;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_MRT_Resolving_deamidations_for_confident_quantification_356c8f4783.jpg" alt="Waters SELECT SERIES MRT: Resolving deamidations for confident quantification. A) The deamidated forms of peptide HC:T23 elute in the tail of the native peptide peak. Without the ultra-high resolution of the SELECT SERIES MRT as shown in (B) identification and quantification of the deamidations." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_MRT_Resolving_deamidations_for_confident_quantification_356c8f4783.jpg 245w," sizes="100vw" width="1024" height="570"></figure><div><div id="text-1092d8da82" data-locator="text"><h3 data-locator="modal-title"><strong>Redefining clarity in mass spectrometry imaging</strong></h3><p>The SELECT SERIES MRT reduces analysis times by providing crystal clear, high-resolution images, acquired at fast imaging speeds without compromising mass spectral resolution or accuracy. This enables accelerated mass spectrometry imaging experiments while still resolving analytes from interferences, revealing fine structural information. The cutting-edge technology and intuitive software combine to revolutionize mass spectrometry imaging for a wide range of applications; where the ability to map compounds that differ by only a few mDa is changing the pace of research. This includes:</p><ul><li>Tissue analysis in biomedical research</li><li>Forensic research, including fingerprint analysis</li><li>Surface analysis, ranging from surface contamination analysis for semiconductor production, to analysis of tape stripped skin samples in the cosmetics industry</li></ul><div><div id="text-7f1b1e82f7" data-locator="text"><h3 data-locator="modal-title"><strong>Maximize MS imaging capabilities with DESI and MALDI</strong></h3><p>Maximize your mass spectrometry imaging capabilities and analytical flexibility with both DESI and MALDI on the SELECT SERIES MRT. Pair the SELECT SERIES MRT with <a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/nav.htm?cid=135047466" data-di-id="di-id-5d203d9f-b105e917">DESI XS</a>&nbsp;for high quality data and clear visualization of molecular spatial distributions. The speed and versatility of DESI imaging makes it best suited to the analysis of lipids and other small molecules under ambient, direct analysis conditions, without the need for additional matrices.</p><p>Use the high-performance MALDI source to improve imaging performance and ease-of-use, so you can rapidly image large tissue sections at high spatial resolution with improved selectivity and sensitivity. MALDI’s design uses a tightly focused laser beam profile, increasing signal-to-noise at high spatial resolutions and providing lateral resolution of 15 µm without oversampling, with data acquisition rates &gt;10 pixels per second, so scientists can acquire high intensity images quickly and efficiently. With MALDI and SELECT SERIES MRT, you can:</p><ul><li>Enable imaging of large sections with a full-sized Micro Titre Plate (MTP) format MALDI target plate</li><li>Access high spatial resolution through increased signal to noise (S/N), with a focused laser beam profile</li><li>Simplify usability with an automated isolation valve and laser focus control</li><li>Maximize productivity and uptime with easy cleaning and maintenance</li></ul><figure class="image"><img style="aspect-ratio:1349/1093;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_MRT_2_8ead9924a6/Waters-SELECT-SERIES-MRT-2.jpg" alt="Waters SELECT SERIES MRT." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_MRT_2_8ead9924a6/thumbnail_Waters-SELECT-SERIES-MRT-2.jpg 193w," sizes="100vw" width="1349" height="1093"></figure></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

Waters Arc Premier LC System

The Arc Premier System features new, innovative MaxPeak High Performance Surfaces (HPS) Technology, which effectively reduces non-specific adsorption due to metal interactions, without complicated mobile phases or laborious methods.

<div><div><h4><strong>See clearly and consistently, with confidence.</strong></h4></div></div><div><div><p><strong>Today’s scientists and lab managers need to confidently produce the most accurate, reliable, and reproducible data. The Arc Premier System produces correct, robust results from the very first injection, reliably and easily.&nbsp;</strong></p><p>The <strong>Arc Premier System</strong> features the new, innovative solution of <strong>MaxPeak High Performance Surfaces (HPS) Technology</strong>, which effectively reduces non-specific adsorption due to metal interaction — without complicated mobile phases or laborious methods. The <strong>Arc Premier System</strong> sharpens chromatographic peaks for <strong>metal-sensitive compounds</strong> by eliminating secondary interactions from metals, resulting in:</p><ul><li>Improved compliance from higher quality data</li><li>Faster, more reproducible, and accurate data integration</li><li>Shorter time from sample to result, without conditioning</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_6d7c95a93a.jpg" alt="Waters Arc Premier LC System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_6d7c95a93a.jpg 245w," sizes="100vw" width="1024" height="611"></p><p>With the Arc Premier System, we continue to deliver on the product quality you expect, combined with recent innovations in <strong>MaxPeak HPS Technolog</strong>y, to always deliver the<strong> best possible results</strong>.&nbsp;</p><p>Whether you are working to ensure the safety of a new and innovative product, maintain the consistency and supply of a current one, or test your food safety and quality, the Arc Premier System gives you<strong> greater data quality, flexibility, reproducibility, and faster data integration </strong>with right first-time results right out of the box, <strong>without the need for complicated mobile phases and laborious methods to prepare your system.</strong></p><div><div><div><div><div><div><ul><li><strong>MaxPeak HPS Technology</strong>, minimizing risk of metal-analyte interactions</li><li>Pressure envelope extends to <strong>9500 psi </strong>through a flow rate range of <strong>1–5 mL/min</strong>, providing an optimized pairing with column dimension and particle size</li><li><strong>Flexible quaternary and binary pumping options</strong></li><li>High performance analytical optical detectors, include photodiode array (PDA), UV/Vis, and fluorescence (FLR)</li><li>Mass detection options with the <strong>ACQUITY™ QDa™ Mass Detector</strong>,<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1543"> <strong>SQD2 Detector</strong></a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/209"><strong>Xevo™ TQ-S micro</strong></a>, and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/212"><strong>Xevo TQ-S cronos</strong></a></li><li>Flexible column manager options available for up to six columns ranging in length from <strong>50 mm to 300 mm</strong>, with a temperature range of <strong>4 °C to 90 °C</strong></li><li><strong>Auto•Blend Plus™ Technology</strong> for mobile phase reproducibility and <strong>Gradient SmartStart</strong> for method transfer</li><li>Wide range of trusted particle technologies in <strong>MaxPeak Premier Columns</strong></li><li>Compliance-ready<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627"><strong>Empower™ Chromatography Data System</strong> </a>(CDS) delivering high quality data integrity</li><li>Automated quantification tools to minimize compliance costs and time</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_2_d1a922ae6d.jpg" alt="Waters Arc Premier LC System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_2_d1a922ae6d.jpg 245w," sizes="100vw" width="1024" height="585"></p></div></div><div><div><h3><strong>Flexible and Robust HPLC Designed for Your Development and Routine Testing Needs</strong></h3><ul><li><strong>Low dispersion HPLC: </strong>High performing HPLC with bandspread of &lt;30 μL for high chromatographic resolution.&nbsp;</li><li><p><strong>Versatile column management: </strong>Support for analytical LC column dimensions up to 300* mm with capacity to automatically select between as many as six analytical columns. Equipped with independently controlled temperature zone, active pre-heating, and<strong> eCord™ Intelligent Chip Technology </strong>for Waters columns.</p><p>* MaxPeak Premier Columns are recommended for use with the Arc Premier System and are currently only available in lengths up to 150 mm.</p></li><li><strong>Flow-through needle Injector: </strong>Volume range of 0.1 to 1000 μL and ultra-low carryover performance compatible with sensitive LC-MS applications.</li><li><strong>High Performance Surfaces Technology: </strong>With MaxPeak HPS Technology, analytes are impeded from Lewis acid-base interactions and adsorptive losses. This technology has proven to be effective in improving the analysis of electron-rich compounds, such as those containing phosphate and carboxylate groups.</li><li><strong>Consumables: </strong>Compatible with MaxPeak Premier Column chemistry to meet the needs of your reversed-phase and HILIC applications. Quality Control (QC) Reference Material benchmarks your Arc Premier System and enables perfect resolution. <strong>Certified clean </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/197"><strong>QuanRecovery™ vials</strong></a> to give full sample integrity.</li><li><strong>High performance analytical detectors : </strong>A wide range of high-performance detection capabilities, optimized with HPS, to support diverse applications. Includes photodiode array, UV/Vis, and fluorescence, with the additional option of single and triple quad mass detection.</li><li><strong>Solvent blending: </strong>Quaternary or binary solvent mixing. Delivers precise and accurate separations at pressures up to 9500 psi. Optional solvent select valve adds access to additional solvents. Automated solvent compressibility, integrated solvent degassing, and programmable seal wash maximize flow accuracy, precision, and reliability.</li><li><strong>Auto•Blend Plus Technology: </strong>Reduce human error and accelerate method robustness testing for chromatographic methods by programming gradients to control mobile phase pH and ionic strength.</li><li><strong>Gradient SmartStart: </strong>Adjust the injection relative to the gradient start to emulate other HPLC systems’ dwell volumes, without the need to alter the gradient table. Successfully transfer most methods in just two injections.</li><li><strong>Laboratory Informatics: </strong>Software control within your existing <strong>Empower </strong>or <strong>MassLynx™ </strong>infrastructure. Empower Method Validation Manager (MVM) allows you to perform chromatographic method validation in one application. Optional S-Matrix Fusion Software automates your method development according to AQbD guidelines.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_3_5db411b90a.jpg" alt="Waters Arc Premier LC System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_3_5db411b90a.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div></div></div><div><div><div><div><div><div><h3><strong>The Universal Platform for Liquid Chromatography</strong></h3><p>Non-specific adsorption of metal-sensitive compounds is an unpredictable challenge, resulting in chromatography with large relative standard deviations (RSDs) and broad, overlapping peaks, which can be challenging to integrate quickly and be accurate consistently.</p><p>Designed with MaxPeak HPS Technology and paired with <strong>MaxPeak Premier 2.5 μm Columns</strong>, the Arc Premier System improves data quality for challenging compounds, including metal-sensitive compounds tested with LC methods – so you can see clearly and consistently, with confidence.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_8_a4038daa52.jpg" alt="Waters Arc Premier LC System: Figure 1. Analysis of adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate. Significant improvements to sensitivity and recovery are observed with the Arc Premier Solution compared to a conventional LC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_8_a4038daa52.jpg 245w," sizes="100vw" width="1123" height="520"></p></div></div></div></div></div></div></div></div><div><div><h3><strong>Clear</strong></h3><p>Future-proof your lab for the next generation of challenging compounds with MaxPeak HPS Technology, which sharpenspeaks for metal-sensitive compounds, resulting in higher data quality and improved sensitivity with chromatographic reproducibility and precision.</p><p>Data quality and performance are <strong>critical </strong>for your lab, but those can’t come at the sacrifice of ruggedness and ease of use.&nbsp;</p><p>With MaxPeak HPS Technology and flexible LC performance, your lab gets the typical benefits of an LC system optimized for <strong>2.x μm column chemistry </strong>with the MaxPeak HPS Technology’s ability to shorten run times and achieve sharp peaks - no lengthy and complex conditioning and conditioning methodologies for metal-sensitive compounds required.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_9_34ec4befec.jpg" alt="Waters Arc Premier LC System: Figure 2. Analysis of adenosine triphosphate. Lower limits of detection and increased calibration curve linearity are demonstrated with the Arc Premier Solution compared to a conventional LC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_9_34ec4befec.jpg 245w," sizes="100vw" width="940" height="523"></p></div></div><div><div><h3><strong>Consistent</strong></h3><p>Seeing your results more clearly than ever is critical, but those results must be consistent and error-free and your methods easily reproducible, so you can spend less time on analysis and ensure compliance.&nbsp;</p><p>By <strong>significantly sharpening peak shapes</strong> for metal-sensitive analytes, scientists report an improvement in %RSD when integrating data from metal-sensitive compounds on the Arc Premier System. Along with the improvement in data consistency users also report a decrease of time needed to integrate peaks by <strong>approximately 30%</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_f80500f8a4.jpg" alt="Waters Arc Premier LC System: Figure 3. Analysis of hydrocortisone phosphate, betamethasone phosphate, dexamethasone, and dexamethasone acetate. Peak heights increased and peak shapes are more symmetrical for metal- sensitive compounds on the Arc Premier Solution compared to a conventional LC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_f80500f8a4.jpg 235w," sizes="100vw" width="1381" height="918"></p><p>The Arc Premier System <strong>eliminates </strong>the need for<strong> variability-prone conditioning and complex mobile phases</strong> – so you can minimize user errors, improve compliance, and reduce expensive audits from red flag-inducing conditioning injections.</p><p>Your organization needs to reliably migrate higher-quality methods between your systems for maximum reproducibility. With MaxPeak HPS Technology and flexible pump configurations, your lab can easily, reliably <strong>migrate methods between MaxPeak HPS-based systems </strong>quickly, saving you time and money from unnecessary rework. You can also<strong> migrate methods from non-MaxPeak HPS-enabled systems </strong>safely, as method performance of non-metal-sensitive compounds have been shown to not be adversely affected.</p><p>Combining higher data quality with ease of use for your analysts and seamless method transfer, enables results that can be generated consistently between runs and with various analysts.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_6_5332aab31f.jpg" alt="Waters Arc Premier LC System: Figure 4. Analysis of hydrocortisone phosphate, betamethasone phosphate, dexamethasone, and dexamethasone acetate. A zoom in on the baseline shows improved peak shapes and resolution for metal-sensitive compounds on the Arc Premier Solution compared to a conventional LC system, improving baseline resolution." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_6_5332aab31f.jpg 229w," sizes="100vw" width="1380" height="942"></p><h3><strong>Confident</strong></h3><p><strong>Reducing analytical uncertainty and achieving peace</strong> of mind are important for your organization. With MaxPeak HPS Technology, metal-sensitive analytes become visible that were previously undetectable. This allows you to quickly and confidently know that you’ve seen everything in your sample, giving you the confidence to stand behind your data-driven decisions.</p><p>The <strong>combination of MaxPeak HPS Technology, flexible pump and column management options, and precise optical and MS detection</strong> give you the method development and verification capabilities to know that no matter the sample - you can deliver a robust method you can stand behind.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_2_85f2f7cc29.jpg" alt="Waters Arc Premier LC System: Figure 5. Analysis of data of hydrocortisone phosphate, betamethasone phosphate, dexamethasone, and dexamethasone acetate from an Arc Premier System and a conventional LC system by six users. Peak area reproducibility improved 9 – 124x when integrating the same data from metal-sensitive compounds across six users." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_2_85f2f7cc29.jpg 239w," sizes="100vw" width="1243" height="810"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_5_514749f32d.jpg" alt="Waters Arc Premier LC System: Figure 6. Analysis of acetaminophen, caffeine, leucine enkephalin, sulfadimethoxine, amitriptyline, reserpine, and terfenadine shows that non-metal-sensitive compounds demonstrate comparable performance between the Arc Premier Solution and conventional LC systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_5_514749f32d.jpg 245w," sizes="100vw" width="1242" height="735"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_7_edc8ecaa93.jpg" alt="Waters Arc Premier LC System: Figure 7. Comparison of adenosine triphosphate and adenosine diphosphate peaks using a conventional LC system and the Arc Premier Solution over 120 injections. The peak areas of metal-sensitive compounds remain constant from the first to the 120th injection using the Arc Premier Solution. Peak areas of metal-sensitive compounds on a conventional LC system continuously increase over time and do not stabilize even after 120 injections." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_7_edc8ecaa93.jpg 218w," sizes="100vw" width="1038" height="744"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_3_f1cca337bc.jpg" alt="Waters Arc Premier LC System: Figure 8. Comparison of adenosine triphosphate and adenosine diphosphate peaks using a conventional LC system and the Arc Premier Solution over 120 injections. The peak areas of metal-sensitive compounds remain constant from the first to the 120th injection using the Arc Premier Solution showing less than a 1% change in peak area for all compounds. Peak areas of metal-sensitive compounds on a conventional LC system show an increase of 2–35% when compared to the first injection and show no signs of stabilization." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_3_f1cca337bc.jpg 216w," sizes="100vw" width="1039" height="750"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_4_1dae3909ae.jpg" alt="Waters Arc Premier LC System: Figure 9. Adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, and adenosine stability using the Arc Premier Solution over 1000 injections. The peak areas of metal-sensitive compounds remain consistent over 1000 injection using the Arc Premier Solution demonstrating the robustness of the system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_4_1dae3909ae.jpg 243w," sizes="100vw" width="1040" height="669"></p></div></div></div></div>

Waters SEC Columns

Get unmatched batch-to-batch reproducibility with the wide range of Waters Size-Exclusion Chromatography (SEC) Columns and standards for protein and peptide characterization.

<h4><strong>Size-based separations of peptides, proteins, nucleic acids, LNPs, and viral vectors</strong></h4><p>Get unmatched batch-to-batch reproducibility with the wide range of <strong>Waters Size-Exclusion Chromatography (SEC) Columns</strong> and standards for protein and peptide characterization.</p><p><strong>SEC</strong> is a powerful technique for aggregate and fragment analysis in the <strong>research, development</strong>, and <strong>manufacturing of biotherapeutic proteins</strong>, such as <strong>insulin</strong> and <strong>monoclonal antibodies</strong> (mAb). With SEC, also known as gel filtration, you can separate proteins based on their size (hydrodynamic radii versus absolute molecular weight) in solution, with larger sized species eluting before smaller proteins.</p><p>The <strong>pore size and volume</strong> of the SEC particles used in the packed column drives this <strong>isocratic based technique</strong>, so the column you choose matters. As the molecular weight of the proteins of interest increases, SEC columns with comparatively larger pore size particles are selected.</p><h4><strong>Overview</strong></h4><ul><li>Gain efficiency with this “platform based” method for <strong>proteins ranging from 10,000 to 650,000 Daltons</strong>, leveraging <strong>MaxPeak Premier Technology</strong> coupled with <strong>BEH-PEO Particle Technology&nbsp;</strong> &nbsp;&nbsp;</li><li><strong>Minimize non-desired secondary interactions </strong>for improved separation reproducibility and results with Ethylene Bridged Hybrid (BEH) SEC particles</li><li>Ensure reliable <strong>column-to-column and batch-to-batch performance</strong> with columns quality control tested with relevant peptide, protein, and mAb samples &nbsp;</li><li>Achieve reliable <strong>high throughput analysis on UPLC</strong> systems with our <strong>1.7 µm particle size</strong> offering and reproducible separations on any <strong>HPLC, UHPLC, or UPLC system</strong> to address different application needs with <strong>2.5 µm&nbsp;</strong></li><li><strong>Extend analytical column life</strong> and<strong> lower cost per analyses </strong>with SEC Guards&nbsp;</li><li>Confirm on-site SEC column and LC system performance with available BEH125, BEH200, BEH450, and mAb Size Variant Standards &nbsp;&nbsp;</li><li><strong>Manufactured in a cGMP, ISO 9001</strong> certified plant using stringent manufacturing protocols and ultra-pure reagents</li></ul><p><strong>Recommended Use: </strong>For size-based peptide, protein, and viral vector analyses.</p><h4><strong>System Dispersion Specifications for Column Recommendations</strong></h4><h5><strong>LC System Dispersions &lt;20 µL (UPLC)</strong></h5><ul><li>ACQUITY Premier Protein SEC 250 Å Guards and Columns</li><li>ACQUITY UPLC Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>1.7 µm guards and columns for low dispersion UPLC systems</li></ul><h5><strong>LC System Dispersions &gt;20 µL – ≤35 µL (UHPLC)</strong></h5><ul><li>XBridge Premier Protein SEC 250 Å Guards and Columns</li><li>XBridge Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>BioResolve mAb SEC Guards and Columns</li><li>2.5 µm guards and columns for UHPLC systems</li></ul><h5><strong>LC System Dispersions &gt;35 µL (HPLC)</strong></h5><ul><li>XBridge Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>XBridge Premier Protein SEC 250 Å Guards and Columns</li><li>BioResolve mAb SEC Guards and Columns</li><li>BioSuite SEC Guards and Columns</li><li>3.5 µm guards and columns for HPLC systems</li></ul><h4><strong>GTxResolve SEC Columns</strong></h4><h5><strong>GTxResolve Premier BEH SEC 450 Å Columns</strong></h5><ul><li>2.5 µm particle size columns for UHPLC and UPLC separations</li><li>Lower secondary interactions and lower noise suitable for platform methods</li><li>Low adsorption hardware necessary for analysis of biologics samples</li><li>450 Å pore size with up to 50% higher resolving power reducing need for sample consumption, resulting in faster analyses<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/GTxResolve-sec-columns.html"><strong>Explore GTxResolve SEC Columns</strong></a></li></ul></li></ul><h5><strong>GTxResolve Premier SEC 1000 Å Columns</strong></h5><ul><li>3 µm columns for UHPLC and UPLC separations</li><li>1000 Å columns with high efficiency for aggregate analysis of GTx drugs with diameter of 80-200 nm</li><li>Low secondary interaction &amp; improved compatibility with universally applied detector, such as MALS</li><li>Low adsorption hardware necessary for analysis of biologics samples<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/GTxResolve-sec-columns.html"><strong>Explore GTxResolve SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_G_Tx_Resolve_SEC_Columns_1b8741a62b.jpg" alt="Waters: GTxResolve SEC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_G_Tx_Resolve_SEC_Columns_1b8741a62b.jpg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Protein SEC Columns</strong></h4><h5><strong>ACQUITY Premier Protein SEC 250 Å Guards and Columns</strong></h5><ul><li>Sub-2-μm particle columns for UPLC separations</li><li>250 Å pore size</li><li>High throughput &amp; resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on low dispersion UPLC Systems</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/acquity-premier-columns.html"><strong>Explore ACQUITY Premier Protein BEH SEC Guards and Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Premier_Columns_featuring_Max_Peak_HPS_Technology_11a541350e.jpg" alt="Waters: ACQUITY Premier Columns featuring MaxPeak HPS Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Premier_Columns_featuring_Max_Peak_HPS_Technology_11a541350e.jpg 234w," sizes="100vw" width="1024" height="682"></p><h5><strong>XBridge Premier Protein SEC 250 Å Guards and Columns</strong></h5><ul><li>2.5 μm particle columns for HPLC and UHPLC separations</li><li>250 Å pore size</li><li>High resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on any LC system</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology</li><li>Specifically designed and QC tested with NIST-based, mAb Size Variant Standard to help ensure batch-to-batch consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/Waters-XBridge-Premier-Columns/nav.htm"><strong>Explore XBridge Premier Protein BEH SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_Premier_Columns_0230c90278.jpg" alt="Waters: XBridge Premier Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_Premier_Columns_0230c90278.jpg 193w," sizes="100vw" width="950" height="768"></p><h5><strong>BioResolve mAb SEC Guards and Columns</strong></h5><ul><li>2.5 μm particle columns for UPLC, UHPLC, and HPLC separations</li><li>200 Å pore size</li><li>Obtain reproducible, accurate quantitation of monoclonal antibodies (mAbs), associated high molecular weight aggregates (&gt;300,000 Da), and lower molecular weight fragments (e.g., &lt;100,000 Da)</li><li>Each packed column is tested with NIST-based, mAb size variant standard to help ensure batch-to-batch and column-to-column performance consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/BioResolve-SEC-mAb-Consumables-%7C-SEC-Guards/nav.htm"><strong>Explore BioResolve mAb SEC Guards and Columns</strong></a></li></ul></li></ul><h5><strong>ACQUITY UPLC Protein BEH SEC Guards and Columns</strong></h5><ul><li>Sub-2-μm particle columns for UPLC separations (2.5 μm for 450 Å)</li><li>Pore size options include: 125 Å, 200 Å, and 450 Å</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency</li><li>10x faster than traditional HPLC-based SEC<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/acquity-uplc-columns.html"><strong>Explore ACQUITY UPLC Protein BEH SEC Guards and Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Acquity_UPLC_kolony_1dc5c25d3e/Waters-Acquity-UPLC-kolony.jpg" alt="Waters: Acquity UPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Acquity_UPLC_kolony_1dc5c25d3e/thumbnail_Waters-Acquity-UPLC-kolony.jpg 245w," sizes="100vw" width="807" height="407"></p><h5><strong>XBridge Protein BEH SEC Columns</strong></h5><ul><li>2.5 μm particle columns for use on any UPLC, UHPLC, or HPLC system</li><li>Pore size options include: 125 Å, 200 Å, and 450 Å</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/XBridge-BEH-Columns-for-High-pH-Chromatography/nav.htm"><strong>Explore XBridge Protein BEH SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_Columns_b0b55de501/Waters-XBridge-Columns.jpg" alt="Waters: XBridge Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_Columns_b0b55de501/thumbnail_Waters-XBridge-Columns.jpg 203w," sizes="100vw" width="900" height="691"></p><h4><strong>Peptide SEC Columns</strong></h4><h5><strong>BioSuite SEC Columns</strong></h5><ul><li>Silica-based diol SEC columns</li><li>Molecular weight exclusion limits are determined primarily by the selected pore size</li><li>Available in analytical and semi-preparative column sizes<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/BioSuite-Columns/nav.htm"><strong>Explore BioSuite SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Bio_Suite_Columns_bf2bb8249f.jpg" alt="Waters: BioSuite Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Bio_Suite_Columns_bf2bb8249f.jpg 184w," sizes="100vw" width="307" height="260"></p><h5><strong>Protein-Pak SEC Columns</strong></h5><ul><li>Based on a 10 μm diol-bonded silica</li><li>Resolve proteins that differ in molecular weight by a factor of two</li><li>Distinguish proteins differing by as little as 15% in molecular weight<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/Protein-Pak-HPLC-and-UPLC-Columns/nav.htm"><strong>Explore Protein-Pak SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Protein_Pak_Columns_be6a669326.jpg" alt="Waters: Protein-Pak Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Protein_Pak_Columns_be6a669326.jpg 245w," sizes="100vw" width="500" height="315"></p><h4><strong>Choose the right SEC Column for your lab</strong></h4><p>SEC is ideal for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic peptides, proteins, and viral vectors, but choosing the right column for your analysis is important. Use the following criteria to choose a column that provides reliable, high quality SEC analyses of various biomolecules, including insulin, monoclonal antibodies, and adeno associated viruses (AAVs).</p><h5><strong>What to consider when choosing an SEC Column:</strong></h5><ul><li>What is the molecular weight of your sample?</li><li>What is the dispersion of your LC system?</li><li>Are you separating components &lt;2 fold difference in molecular weight (MW)?</li><li>Are you separating components &gt;2 fold difference in MW?</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Choose_the_right_Size_exclusion_chromatography_SEC_column_for_your_analysis_with_Waters_SEC_columns_selection_guide_b81f674f65.jpg" alt="Waters: Choose the right Size-exclusion chromatography (SEC) column for your analysis with Waters SEC columns selection guide." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Choose_the_right_Size_exclusion_chromatography_SEC_column_for_your_analysis_with_Waters_SEC_columns_selection_guide_b81f674f65.jpg 158w," sizes="100vw" width="1024" height="1013"></p><h4><strong>Addressing the complexity of SEC Separations</strong></h4><p>Experience the advancements in SEC-based technologies to address non-desired specific ionic and hydrophobic interactions for more reliable results with ACQUITY Premier Protein SEC and XBridge Premier Protein SEC Columns and Guards.</p><h5><strong>With MaxPeak Premier SEC Columns, you can:</strong></h5><ul><li>Reproducibly resolve injected sample components that are less than 2x different in size for challenging applications</li><li>Use a ‘platform SEC method’ to reduce method development time and cost</li><li>Ensure batch-to-batch and column-to-column performance</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Classes_of_therapeutic_proteins_separated_on_an_ACQUITY_Premier_Protein_SEC_Column_d3f74a8ea4.jpg" alt="Waters: Classes of therapeutic proteins separated on an ACQUITY Premier Protein SEC Column." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Classes_of_therapeutic_proteins_separated_on_an_ACQUITY_Premier_Protein_SEC_Column_d3f74a8ea4.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters Atlantis Premier BEH Z-HILIC Column

Waters Atlantis Premier BEH Z-HILIC Columns provide an increase in polar analyte retention and offer a different selectivity when compared to other HILIC chemistries, both powerful attributes when performing HILIC methods development.

<p><strong>Atlantis Premier BEH Z-HILIC Columns </strong>provide an increase in polar analyte retention and offer a different selectivity when compared to other HILIC chemistries, both powerful attributes when performing HILIC methods development. &nbsp;Built using rugged <strong>ethylene bridged hybrid particles</strong>, these columns are <strong>pH stable from pH 2-10</strong>; the MaxPeak High Performance Surface hardware technology significantly reduces unwanted analyte/surface interactions that can lead to poor peak shape and losses in signal intensity.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_Premier_BEH_Z_HILIC_kolony_1_59d95946d7/Waters-Atlantis-Premier-BEH-Z-HILIC-kolony-1.jpg" alt="Waters: Atlantis Premier BEH Z-HILIC column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_Premier_BEH_Z_HILIC_kolony_1_59d95946d7/thumbnail_Waters-Atlantis-Premier-BEH-Z-HILIC-kolony-1.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specification</strong></h4><ul><li><strong>Chemistry:</strong> Sulfobetaine</li><li><strong>Separation Mode: </strong>Hydrophilic Interaction (HILIC)</li><li><strong>Particle Substrate: </strong>Hybrid</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max:</strong> 10 pH</li><li><strong>Maximum Pressure:</strong> 18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>No</li><li><strong>Bonding Technology:</strong> Zwitterion</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Particle Size:</strong> 1.7 µm</li><li><strong>Endfitting Type: </strong>Parker-style</li><li><strong>Pore Size: </strong>95 Å</li><li><strong>Format: </strong>Premier Column</li><li><strong>Surface Area:</strong> 270</li><li><strong>System:</strong> UHPLC, UPLC</li><li><strong>Particle Technology: </strong>BEH</li><li><strong>USP Classification:</strong> L122</li><li><strong>Carbon Load: </strong>17 %</li></ul><h3><strong>Atlantis Premier Columns</strong></h3><h4><strong>Next-generation hybrid particle and surface technology for increased polar analyte retention</strong></h4><p>Retaining polar compounds is one of the most challenging chromatographic problems. With the hybrid particle technology in Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns, those problems are a thing of the past. These reversed-phase, anion-exchanged, mixed-mode, and zwitterionic columns are the next evolution of columns in the Atlantis family, all designed to achieve superior polar compound retention.</p><p>Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns combine a new and innovative chromatographic particle technology with proprietary MaxPeak High-Performance Surfaces (HPS) Technology to boost retention of polar acidic compounds by 50% while eliminating unwanted analyte-surface interactions.</p><h4><strong>Atlantis Premier Column Characteristics</strong></h4><h5><strong>Atlantis Premier BEH 95 Å Particle Columns</strong></h5><ul><li>Mixed-mode C18 Anion-exchange (AX) Chemistry</li><li>Increase retention of polar acids</li><li>Wider pH range (2–10)</li><li>Batch-to-batch reproducibility</li><li>Mass Spectrometry (MS) compatible</li></ul><h5><strong>Atlantis Premier BEH Z-HILIC Columns</strong></h5><ul><li>Uses sulfobetaine zwitterionic ligand</li><li>Increase retention of strongly polar analytes</li><li>Wider pH range (2–10)</li><li>Batch-to-batch reproducibility</li><li>Mass Spectrometry (MS) compatible</li></ul>

Waters Xevo TQ Absolute Triple Quadrupole Mass Spectrometer

The Xevo™ TQ Absolute combines Absolute performance, efficiency, productivity, and confidence to give you the Absolute Power to quantitate your most challenging compounds and make fast decisions.

<h3><strong>Quantitate with Absolute Power</strong></h3><ul><li><strong>Harness Absolute Power for Confident, Fast Decisions</strong></li></ul><p><strong>The Xevo™ TQ Absolute combines Absolute performance, efficiency, productivity, and confidence to give you the Absolute Power to quantitate your most challenging compounds and make fast decisions.</strong></p><h4><strong>Absolute&nbsp;Performance</strong></h4><p>Achieve lower limits of quantitation to meet regulatory requirements with up to <strong>15x more sensitivity</strong> for challenging negative ionizing compounds than the previous product.</p><h4><strong>Absolute Efficiency &nbsp;</strong></h4><p>Achieve improved environmental sustainability and lower your laboratory operational costs with an instrument that uses <strong>up to 50% less electricity and gas and produces 50% less heat</strong> than most other high performance TQ mass spectrometry instruments on the market.</p><h4><strong>Absolute&nbsp;Productivity</strong></h4><p>Boost your productivity with flexible software solutions, including waters_connect,™ the modern, intuitive informatics solution that can<strong> save up to 50% of your data review time </strong>while facilitating data integrity.&nbsp;</p><h4><strong>Absolute Confidence</strong></h4><p>Ensure consistent robustness and reproducibility so you can <strong>maintain performance and productivity </strong>over a longer time period.</p><h3><strong>Absolute Performance</strong></h3><h4><strong>Increased sensitivity with lower limits of quantitation</strong></h4><p>One of the largest challenges facing many labs is the need to achieve lower limits of quantitation and meet regulatory requirements, particularly for challenging negative ionizing compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Waters_Xevo_TQ_Absolute_poskytuje_6x_15x_lepsi_citlivost_nez_jeho_predchudci_6ae9af3f1d/Waters-Corporation-Waters-Xevo-TQ-Absolute-poskytuje-6x-15x-lepsi-citlivost-nez-jeho-predchudci.webp" alt="Waters Xevo TQ Absolute Triple Quadrupole Mass Spectrometer: *Xevo TQ Absolute shows up to 15x better ion response as compared to the Xevo TQ-XS and up to 6x better ion response than another leading high-performance LC-MS/MS instrument." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Waters_Xevo_TQ_Absolute_poskytuje_6x_15x_lepsi_citlivost_nez_jeho_predchudci_6ae9af3f1d/thumbnail_Waters-Corporation-Waters-Xevo-TQ-Absolute-poskytuje-6x-15x-lepsi-citlivost-nez-jeho-predchudci.webp 245w," sizes="100vw" width="1024" height="540"></p><p>Ensure you have the confidence in your data to make decisions fast with an instrument that provides Absolute Performance. <strong>Xevo TQ Absolute delivers up to 15x better sensitivity for challenging negative ionizing compounds</strong> than the previous product, so you can make decisions quickly and efficiently.</p><h3><strong>Absolute Efficiency</strong></h3><h4><strong>Lower operational costs and improved sustainability</strong></h4><p>As a leading technology innovator in chromatography, mass spectrometry, and thermal analysis instruments and software, Waters shares a common mission with our customers: to leave the world a better place than we found it.&nbsp;</p><p>Many of our customers are actively working to address environmental sustainability in balance with the need to continually reduce operational costs. During development of the Xevo TQ Absolute, Waters has addressed these challenges by providing an instrument with lower electricity consumption, gas consumption, and BTU/hr output – lessening the need for air conditioning and lowering costs.</p><p>The Xevo TQ Absolute also provides more analytical capability per square foot of lab space by minimizing the LC-MS/MS footprint, enabling laboratory managers to fully optimize their facilities and increase efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Xevo_TQ_Absolute_Triple_Quadrupole_Mass_Spectrometer_Absolute_Efficiency_1b0bcac3ac.jpg" alt="Waters Xevo TQ Absolute Triple Quadrupole Mass Spectrometer: Absolute Efficiency." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Xevo_TQ_Absolute_Triple_Quadrupole_Mass_Spectrometer_Absolute_Efficiency_1b0bcac3ac.jpg 206w," sizes="100vw" width="1050" height="794"></p><p>*Source: <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/content/dam/SCIEX/pdf/customer-docs/site-planning-guide/7500-site-planning-guide-en.pdf">https://sciex.com/</a></p><h3><strong>Absolute Productivity</strong></h3><h4><strong>Achieve more with flexible software solutions&nbsp;</strong></h4><p>In an ever-changing environment, flexibility can help protect against future unknowns. The Xevo TQ Absolute is available on both our waters_connect and MassLynx™ Software platforms, providing the ultimate flexibility to choose a future-proof solution to meet your lab requirements</p><h5><strong>waters_connect</strong></h5><p>Our modern, app-based software platform, waters_connect, delivers efficiency and usability benefits, including the unique Exception Focused Review (XFR) functionality that can save up to 50% of your data review time, ensuring your lab is as productive as possible.</p><h5><strong>MassLynx</strong></h5><p>Our tried and well-established MassLynx Software package delivers market-leading versatility and flexibility for all application areas.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Xevo_TQ_S_micro_Triple_Quadrupole_Mass_Spectrometry_Convert_data_into_meaningful_results_with_waters_connect_and_Mass_Lynx_028cc58bc8.jpg" alt="Waters Xevo TQ Absolute Triple Quadrupole Mass Spectrometer: Convert data into meaningful results with waters_connect and MassLynx." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Xevo_TQ_S_micro_Triple_Quadrupole_Mass_Spectrometry_Convert_data_into_meaningful_results_with_waters_connect_and_Mass_Lynx_028cc58bc8.jpg 245w," sizes="100vw" width="1024" height="346"></p><h3><strong>Absolute Confidence</strong></h3><h4><strong>Tackle your toughest challenges</strong></h4><p>Analytical laboratories face several challenges that are constantly changing, so it’s important to have Absolute Confidence that your analytical instrumentation can keep pace.&nbsp;</p><p>The Xevo TQ Absolute has been specifically developed to deliver consistent robustness and reproducibility, so you can maintain performance and productivity over a longer time period.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Novy_stit_iontoveho_zdroje_hmotnosniho_spektrometru_Waters_Xevo_TQ_Absolute_0ef51bf805/Waters-Corporation-Novy-stit-iontoveho-zdroje-hmotnosniho-spektrometru-Waters-Xevo-TQ-Absolute.webp" alt="Waters Xevo TQ Absolute Triple Quadrupole Mass Spectrometer: New source shield technology featured in the Xevo TQ Absolute Mass Spectrometer minimizes contamination and increases uptime." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Novy_stit_iontoveho_zdroje_hmotnosniho_spektrometru_Waters_Xevo_TQ_Absolute_0ef51bf805/thumbnail_Waters-Corporation-Novy-stit-iontoveho-zdroje-hmotnosniho-spektrometru-Waters-Xevo-TQ-Absolute.webp 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Better by design</strong></h4><p>Optimal probe positioning guidance ensures you can achieve the best possible performance while minimizing contamination and reducing the need for inconvenient downtime to clean the instrument. A new source shield maximizes uptime by minimizing contamination of the source from sample matrix or mobile phase salts.</p><h3><strong>Absolute Support</strong></h3><h4><strong>Ensure your success with world-renowned services</strong></h4><p>Waters Service and Support helps maintain system peak performance, minimize downtime, address scientific application challenges, and support stringent compliance requirements.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Xevo_TQ_Absolute_tandemovy_hmotnostni_spektrometr_2_f0a5cb429c/Waters-Xevo-TQ-Absolute-tandemovy-hmotnostni-spektrometr-2.webp" alt="Waters Xevo TQ Absolute Triple Quadrupole Mass Spectrometer: Absolute support." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Xevo_TQ_Absolute_tandemovy_hmotnostni_spektrometr_2_f0a5cb429c/thumbnail_Waters-Xevo-TQ-Absolute-tandemovy-hmotnostni-spektrometr-2.webp 234w," sizes="100vw" width="1024" height="682"></p><p><strong>Waters award-winning consistent quality support and professional services ensure your success wherever your laboratory is located in the world. With Waters Service and Support offerings* for your Xevo TQ Absolute, you can:</strong></p><h4><strong>Get Up And Running Quicly</strong></h4><p>Get the onsite training and consultation you need to integrate the Xevo TQ Absolute into your laboratory and assist with data migration and method transfer needs with Waters Professional Services.</p><h4><strong>Meet Your Lab’s Unique Requirements&nbsp;</strong></h4><p>Protect your high-performance instrument investment with the comprehensive portfolio of service plans from Waters FlexCHOICE, which provides access to the most in-depth and up-to-date knowledge of the advanced science and technologies within Waters systems.</p><h4><strong>Facilitates Compliance</strong></h4><p>Simplify the process of qualification, validation, and record-keeping so that your business can focus on delivering products to market with Waters Compliance Services – so you can meet the regulations in your industry and in the countries where you conduct business.</p><h4><strong>Reduce Total Cost Of Ownership</strong></h4><p>FlexCAPITAL<strong>** </strong>is designed to support continuous laboratory operation while satisfying the budgeting challenges of a constantly shifting landscape. Minimize total cost of ownership while optimizing instrument performance and protecting your organization from security risks associated with outdated software platforms.</p><h4><strong>Leverage Your Existing Technology</strong></h4><p>With the FlexUP technology renewal program, you can upgrade your older tandem quadrupole mass spectrometry system to the Xevo TQ Absolute – improving performance, results, and productivity while controlling costs and mitigating compliance risk.</p><p><strong>*</strong>Availability of offerings varies by country. Contact Waters or your local Waters representative for details.</p><p><strong>** </strong>FlexCAPITAL payment plan options and availability may vary by country. Other restrictions may apply.</p>

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