<p><strong>This workflow confirms and quantifies 31 drugs of abuse in oral fluid (including THC) in a 4.5‑minute LC run on the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149"><strong>Thermo Scientific Stellar mass spectrometer</strong></a><strong> using full scan + targeted MS2 (tMS2) with both HCD and CID fragmentation. With 1 µL injection and 0.1% formic acid in water/methanol mobile phases, the method supports ~13 samples/hour (up to ~320/day continuous operation) while maintaining confirmation performance via ion ratios.</strong></p><blockquote><h5><strong>Method at a glance</strong></h5><ul><li><strong>What matrix is tested?</strong> Oral fluid</li><li><strong>How many drugs?</strong> 31 drugs of abuse (panel includes THC)</li><li><strong>Run time?</strong> 4.5 minutes</li><li><strong>Throughput?</strong> ~13 samples/hour → up to ~320 samples/day (24‑hour operation)</li><li><strong>Injection volume?</strong> 1 µL</li><li><strong>Mobile phases?</strong> 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B)</li><li><strong>MS acquisition?</strong> Full scan + targeted MS2 (tMS2)</li><li><strong>Fragmentation?</strong> Compound‑optimized <strong>HCD</strong> and <strong>CID</strong> in the same targeted list</li><li><strong>Confirmation strategy?</strong> Ion ratios with ±20% tolerance (typical confirmatory approach)</li></ul></blockquote><h3><strong>Why oral fluid—and why speed matters</strong></h3><p>Oral fluid is attractive for clinical and forensic toxicology because collection is simple and the matrix aligns well with high‑volume workflows (workplace/occupational, roadside, and other settings). The challenge is building a method that’s simultaneously:</p><ul><li><strong>Broad enough</strong> for today’s drug panels</li><li><strong>Sensitive enough</strong> to reach required cutoffs</li><li><strong>Fast enough</strong> to keep sample queues under control</li><li><strong>Confirmation‑ready</strong> (consistent product ions and ion ratios)</li></ul><p>A <strong>4.5‑minute</strong> method is a practical lever here: compress runtime, raise throughput, and still keep confirmation quality by optimizing MS/MS conditions per compound.</p><h3><strong>The LC method</strong></h3><p>A short, efficient separation is the foundation of the workflow:</p><ul><li><strong>UHPLC system:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744"><strong>Thermo Scientific Vanquish Horizon UHPLC</strong></a></li><li><strong>Column:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/424"><strong>Thermo Scientific Accucore Biphenyl</strong></a>, 50 × 2.1 mm, 2.6 µm</li><li><strong>Mobile phase A:</strong> 0.1% formic acid in water</li><li><strong>Mobile phase B:</strong> 0.1% formic acid in methanol</li><li><strong>Injection volume:</strong> <strong>1 µL</strong></li><li><strong>Gradient runtime:</strong> <strong>4.5 minutes</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_fd3f3e8ea4.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 1: Thermo Scientific Vanquish Horizon UHPLC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_fd3f3e8ea4.jpg 120w," sizes="100vw" width="747" height="969"></p><h3><strong>The MS method (Full scan + targeted MS<sup>2</sup> with HCD + CID)</strong></h3><h5><strong>Acquisition approach</strong></h5><p>This method uses a “two‑layer” strategy for quantitation + confirmation:</p><ol><li><strong>Full scan</strong> (broad coverage, context, quantitative support)</li><li><strong>Targeted MS<sup>2</sup> (tMS2)</strong> (confirmation‑focused product ions, ion ratios)</li></ol><h5><strong>The key differentiator: HCD and CID in one targeted list</strong></h5><p>Instead of forcing every analyte into a single fragmentation style, the method uses both:</p><ul><li><strong>HCD</strong> (beam‑type fragmentation)</li><li><strong>CID</strong> (resonance‑type fragmentation)</li></ul><h5><strong>Why that improves method development:</strong></h5><p>Drugs of abuse span multiple chemistries. A single collision approach often works “okay” for most compounds—but one or two analytes can become persistent problems (weak qualifiers, unstable ion ratios, excess noise). Having both <strong>HCD and CID</strong> available in one method lets you optimize per compound and move faster.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_3_e4a95651a2.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 2: Thermo Scientific Stellar mass spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_3_e4a95651a2.jpg 245w," sizes="100vw" width="645" height="377"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_5_88276eec6a.jpg" alt="Thermo Fisher Scientific: 4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS: Figure 3: Full scan &amp; targeted MS2 methods, HCD and collision energies used." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_4_5_Minute_Oral_Fluid_LC_MS_Method_to_Detect_31_Drugs_of_Abuse_Using_Stellar_MS_5_88276eec6a.jpg 245w," sizes="100vw" width="768" height="279"></p><h3><strong>Why CID + HCD flexibility improves confirmation</strong></h3><p>In this workflow, <strong>HCD</strong> performed well for most compounds. But for <strong>buprenorphine</strong>, HCD produced one dominant product ion with additional low m/z noise and noticeable residual precursor. Using <strong>CID</strong> generated two high‑intensity product ions and delivered <strong>consistent ion ratios</strong> across the calibration range.</p><p>When your confirmation hinges on stable ion ratios, the ability to switch fragmentation mode (not just collision energy) is a method‑development advantage—especially for “problem analytes.”</p><h3><strong>Controllable ionization</strong></h3><p>Oral fluid methods often deal with limited sample volume and matrix effects. This workflow pairs low injection volume with ionization control using the Thermo Scientific OptaMax Plus HESI source.</p><ul><li>The OptaMax Plus HESI source supports high probe temperature capability (up to 650 °C).</li><li>In this study, a vaporizer temperature of 500 °C was used to help achieve sensitivity and improve relative peak areas across drugs of abuse.</li></ul><p><strong>Why this matters:</strong> If you’re injecting only 1 µL, ionization stability and efficiency can be the difference between “meets cutoff” and “misses at LOQ.”</p><h3><strong>Sample preparation</strong></h3><p>Oral fluid panels become more challenging when THC is included because most drugs of abuse are basic, while THC is largely neutral, and that can stress extraction chemistry.</p><p>In this workflow, oral fluid calibrators and samples were extracted using a mixed‑mode approach designed to recover THC alongside other drugs:</p><ul><li>Calibrators prepared across eight levels (broad dynamic range)</li><li>Extraction performed using mixed‑mode chemistry tips (SCX/WAX) in a dispersive SPE‑style workflow</li><li>Steps automated on a liquid handling platform to support high‑throughput consistency</li><li>Samples dried and reconstituted prior to LC‑MS analysis</li><li>Only 1 µL injected per run</li></ul><p>Even if your lab uses different automation or consumables, the method structure (fast LC + full scan + targeted MS2 with dual fragmentation) transfers well.</p><blockquote><h5><strong>Throughput math</strong></h5><ul><li><strong>4.5 minutes per injection</strong> → 60 / 4.5 = <strong>13.3 injections/hour</strong></li><li>That’s <strong>~13 samples/hour</strong> in typical phrasing</li><li>Over 24 hours: 13.3 × 24 = <strong>~320 samples/day</strong> (continuous operation)</li></ul><p>If you run a single shift, just scale accordingly (e.g., ~106/day for an 8‑hour shift, excluding QC and overhead).</p></blockquote><h3>Performance summary</h3><h5><strong>Sensitivity and linearity</strong></h5><ul><li><strong>Linearity</strong> from LOQs as low as <strong>0.25 ng/mL</strong> up to <strong>1,000 ng/mL</strong> (panel-dependent)</li><li>For THC specifically: <strong>LOQ at 1 ng/mL</strong>, with a dynamic range extending to <strong>1,000 ng/mL</strong> in this evaluation</li><li>Across the panel, LOQs were reported as <strong>lower than recommended confirmation cutoffs</strong> referenced in SAMHSA guidance and NSC Tier 1 cutoffs (study-specific outcome)</li></ul><h5><strong>Confirmation via ion ratios</strong></h5><ul><li>Ion ratios evaluated using <strong>±20% tolerance</strong> (typical confirmatory acceptance framework)</li><li>The workflow produced <strong>consistent ion ratios across calibrator levels</strong> for the full panel in this evaluation</li></ul><h5><strong>Case sample comparison (real-world relevance)</strong></h5><p>When compared against a validated triple quadrupole method for case study samples, the Stellar MS workflow:</p><ul><li>Confirmed the positive hits observed by the reference method</li><li>Reported a few additional positives for select analytes, attributed to lower LOQs</li><li>Highlighted an operational efficiency point: <strong>1 µL injection</strong> in this method versus <strong>20 µL injection</strong> in the compared method</li></ul><h3><strong>FAQ</strong></h3><div><div><ol><li><strong>How long does the LC‑MS run take for the 31‑drug oral fluid panel?</strong><br><strong>4.5 minutes per injection</strong> using a short UHPLC gradient.<br>&nbsp;</li><li><strong>How many drugs of abuse can Stellar MS detect in this workflow?</strong><br><strong>31 drugs of abuse</strong>, including <strong>THC</strong>, using full scan + targeted MS2.<br>&nbsp;</li><li><strong>What MS/MS approach is used?</strong><br>A combined strategy: <strong>full scan</strong> plus <strong>targeted MS2 (tMS2)</strong> for confirmation.<br>&nbsp;</li><li><strong>Why use both HCD and CID?</strong><br>Because some analytes fragment better—and produce more stable confirmation ions/ion ratios—under one mode versus the other. Using both increases flexibility and speeds method optimization.<br>&nbsp;</li><li><strong>What injection volume is used?</strong><br><strong>1 µL</strong> of extracted sample.<br>&nbsp;</li><li><strong>What mobile phases are used?</strong><br><strong>0.1% formic acid in water (A)</strong> and <strong>0.1% formic acid in methanol (B)</strong>.<br>&nbsp;</li><li><strong>How many samples can you run per day?</strong><br>At 4.5 minutes/injection, throughput is <strong>~13 samples/hour</strong> or <strong>up to ~320/day</strong> with continuous operation (actual throughput depends on QC and scheduling).</li></ol></div></div><h3><strong>What makes this workflow “easy” to develop on Stellar MS</strong></h3><p>Here’s the short list of features that directly reduce method‑development friction:</p><ul><li><strong>HCD or CID</strong> in one method → optimize per compound, not compromise</li><li><strong>Fast MS2 acquisition speeds</strong> (up to 140 Hz for MS2) → more data points across narrow peaks</li><li><strong>Fast polarity switching</strong> (true 5 ms) → supports mixed panels efficiently</li><li><strong>Adaptive RT</strong> (real‑time retention time window adjustment) → helps reduce reinjections when retention times drift (e.g., column aging) and supports method transfer from longer to shorter gradients</li><li><strong>OptaMax Plus HESI</strong> source control → supports sensitivity at low injection volumes</li></ul><p><i>If you’re building a fast oral fluid confirmation workflow—or updating an existing DOA panel—this 4.5‑minute method shows how Stellar MS can combine <strong>speed, confirmation-ready MS2, and fragmentation flexibility (HCD + CID)</strong> in a single targeted workflow. Reach out to learn how to adapt the targeted list, collision settings, and scheduling strategy to your lab’s cutoffs and throughput goals.</i></p><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a><strong>&nbsp;</strong>#MassSpectrometry #OralFluid #DrugsOfAbuse #MethodDevelopment #HighThroughput</p></blockquote>

4.5-Minute Oral Fluid LC MS Method to Detect 31 Drugs of Abuse Using Stellar MS

<p><strong>In our </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7741"><strong>previous article, we introduced the main types of detectors used in HPLC</strong></a><strong>. This time, we take a closer look at the performance characteristics of LC detectors and the specifications that should be considered when assessing whether a detector is suitable for a particular chromatographic separation. We will also compare the most common detector technologies and provide some practical guidance for choosing the right option for a specific application.</strong></p><h3><strong>What Defines an Ideal HPLC Detector?</strong></h3><p>With such a wide variety of HPLC detectors available, an obvious question arises: why is there no single detector that works for every application? The simple answer is that no detector can satisfy every analytical requirement at the same time. Nevertheless, most detector designs aim to achieve a similar set of desirable properties.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Detector_requirements_334605c913.webp" alt="KNAUER: Figure 1 - Detector requirements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Detector_requirements_334605c913.webp 245w," sizes="100vw" width="1024" height="384"></p><h4><strong>1. High Sensitivity – Detecting Very Small Amounts</strong></h4><p>An ideal detector should be capable of measuring very low quantities of analyte, often in the nanogram or even picogram range. High sensitivity leads to lower limits of detection (LOD), can reduce sample preparation requirements, and is particularly valuable for applications such as impurity analysis or pharmacokinetic studies.</p><p>Some detector technologies, including fluorescence detection (FLD), electrochemical detection (ECD), and mass spectrometry (MS), can achieve extremely high sensitivity. However, this level of performance is often limited to specific classes of compounds.</p><h4><strong>2. Good Selectivity or Broad Applicability – Knowing What Is Being Detected</strong></h4><p>Selectivity describes how effectively a detector responds to the target analyte while minimizing interference from the sample matrix or mobile phase.</p><p>Depending on the analytical task, a detector may need to be highly selective, responding only to compounds with specific characteristics such as fluorescence or electrochemical activity. In other cases, broader applicability may be more important, allowing a wide range of compounds to be detected regardless of their molecular structure.</p><h4><strong>3. Linear Response and Broad Dynamic Range – Reliable Quantification</strong></h4><p>A good detector should provide a linear response across a sufficiently wide concentration range. This makes it possible to accurately quantify both major components and trace impurities within the same analytical method.</p><p>A broad linear range simplifies calibration and improves confidence in quantitative results. Detector response should also remain highly reproducible from one injection to another.</p><h4><strong>4. Compatibility with HPLC Conditions – Fitting the Chromatographic Method</strong></h4><p>An ideal detector should be compatible with both gradient and isocratic elution, commonly used mobile phases and additives, typical flow rates, and standard HPLC operating temperatures.</p><p>Poor compatibility may restrict method flexibility or force compromises in chromatographic performance. Some detectors provide excellent analytical performance but only under specific operating conditions, making them less suitable for certain methods.</p><h4><strong>5. Stability and Low Noise – Distinguishing Signal from Background</strong></h4><p>A stable baseline with minimal noise and drift improves the signal-to-noise ratio and overall data quality. This becomes especially important in long chromatographic runs, gradient methods, and trace-level analysis.</p><p>Lower baseline noise makes weak analyte signals easier to distinguish and directly contributes to improved detection limits.</p><h4><strong>6. Robustness, Ease of Use, and Maintenance – Supporting Routine Laboratory Work</strong></h4><p>For day-to-day use, a detector should be reliable over long sequences, straightforward to operate, and require as little maintenance as possible.</p><p>In routine laboratories, robustness and ease of operation can be just as important as analytical performance. Simple troubleshooting, low maintenance requirements, and limited consumable costs are major advantages, particularly in high-throughput environments.</p><h3><strong>The Reality: No Detector Offers Everything</strong></h3><p>Since no detector fulfils all these requirements perfectly, detector selection is inevitably a compromise between sensitivity, selectivity, universality, robustness, operating flexibility, and cost.</p><p>For this reason, multi-detector configurations are increasingly common in modern HPLC systems. Combinations such as UV + CAD or UV + MS make it possible to benefit from complementary detection principles within a single analytical workflow.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_Comparison_of_commonly_used_HPLC_detector_classes_d74817b0f0.jpg" alt="KNAUER: Table 1 - Comparison of commonly used HPLC detector classes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_Comparison_of_commonly_used_HPLC_detector_classes_d74817b0f0.jpg 113w," sizes="100vw" width="806" height="1115"></p><h3><strong>Critical Performance Parameters of HPLC Detectors</strong></h3><h4><strong>Data Sampling Rate</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Example_for_too_less_and_too_high_data_sampling_rate_741a53edc6.webp" alt="KNAUER: Figure 2 - Example for too less and too high data sampling rate." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Example_for_too_less_and_too_high_data_sampling_rate_741a53edc6.webp 245w," sizes="100vw" width="1024" height="410"></p><p>The data acquisition rate, also known as the sampling rate, is one of the most important detector settings in HPLC. It describes how many data points are recorded per second and directly influences peak representation, peak shape, area precision, and baseline noise.</p><p>The higher the sampling rate, the more data points are collected. The detector processes the measurements collected over a defined time interval and averages them, and these averaged points are then used to construct the chromatogram.</p><p>At a data rate of 5 Hz, for example, five data points are acquired every second. If too few points are collected across a chromatographic peak, the resulting peak can appear jagged, poorly defined, and analytically unreliable, as illustrated on the left side of Figure 2.</p><p>At the other extreme, a setting such as 100 Hz records 100 points each second, which is often unnecessarily high for conventional HPLC. The optimum sampling rate mainly depends on chromatographic peak width.</p><p>For most standard HPLC applications, a data rate of approximately <strong>10 to 20 Hz</strong> is sufficient to generate a smooth and symmetrical Gaussian peak.</p><p>One consequence of using an excessively high acquisition rate is increased baseline noise. Statistically, the noise of the system depends on the number of data points used for averaging and decreases with the square root of the number of averaged measurements.</p><p>Selecting an appropriate sampling rate is therefore a balance between collecting enough points to describe the chromatographic peak accurately and avoiding unnecessary noise.</p><h4><strong>Noise and Drift</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Baseline_noise_and_drift_685652e641.webp" alt="KNAUER: Figure 3 - Baseline noise and drift" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Baseline_noise_and_drift_685652e641.webp 245w," sizes="100vw" width="1024" height="400"></p><p>What exactly is baseline noise? In simple terms, it represents the short-term fluctuations of the detector baseline over a defined period and is expressed in the units appropriate for the particular detector.</p><p>Baseline noise is typically determined from the peak-to-peak variation of the detector signal while the mobile phase passes through the system.</p><p>All HPLC detectors exhibit a certain level of noise. Potential sources include:</p><ul><li>electronic noise,</li><li>detector cell noise,</li><li>detector instability,</li><li>temperature fluctuations,</li><li>mobile-phase effects,</li><li>and other environmental or instrumental influences.</li></ul><p>Noise directly affects detector sensitivity because it determines the <strong>signal-to-noise ratio (S/N)</strong>.</p><p>A chromatographic peak is generally considered reliably detectable when its height is at least three times greater than the calculated baseline noise. This relationship is commonly used to define the <strong>limit of detection (LOD)</strong>.</p><p>The <strong>limit of quantification (LOQ)</strong> requires a stronger signal and is typically defined as approximately ten times the baseline noise.</p><p>Another important parameter affecting sensitivity is baseline drift. Drift reflects the gradual change in detector response over time and therefore provides useful information about long-term detector stability.</p><p>It is helpful to distinguish between short-term peak-to-peak noise and longer-term baseline drift over a defined time interval. Drift can be described as the average slope or change of the baseline within a specific period.</p><p>Excessive drift may reduce sensitivity and complicate chromatographic peak integration.</p><p>Short-term baseline instability can be influenced by contamination or deterioration of detector components, while long-term drift is often related to temperature stability, detector equilibration, or changes occurring within the analytical system.</p><h3><strong>How to Select the Right HPLC Detector</strong></h3><p>There is no universally “best” HPLC detector. The correct detector is simply the one that best matches the analytical task.</p><p>When selecting a detector, several practical questions can help narrow the choice.</p><h4><strong>1. Does the analyte absorb UV light or fluoresce?</strong></h4><p>If the answer is yes, optical detectors such as <strong>UV/VIS</strong> or <strong>FLD</strong> are often the simplest and most effective options.</p><p>UV/VIS detection is widely applicable to compounds containing suitable chromophores, while fluorescence detection provides particularly high sensitivity and selectivity for naturally fluorescent or derivatized analytes.</p><h4><strong>2. Does the analyte lack a chromophore?</strong></h4><p>If UV absorption is weak or absent, detectors such as <strong>RID, ELSD, or CAD</strong> may provide a more appropriate solution.</p><p>These detector types are useful for compounds such as sugars, lipids, polymers, and other analytes that are difficult to monitor by conventional UV detection.</p><h4><strong>3. Is structural information required?</strong></h4><p>If structural information is important, <strong>mass spectrometry</strong> is usually the strongest choice.</p><p>MS provides mass-to-charge information and, when tandem mass spectrometry is used, fragmentation data that can support compound identification and structural characterization.</p><h4><strong>4. Is extremely high sensitivity necessary?</strong></h4><p>For applications requiring very low detection limits, <strong>electrochemical detection</strong> or <strong>mass spectrometry</strong> may be the most suitable options.</p><p>The exact choice depends on the chemical properties of the analyte and the required degree of selectivity.</p><h4><strong>5. Are macromolecules such as proteins or polymers being investigated?</strong></h4><p>For characterization of macromolecules, <strong>MALS</strong> and <strong>viscometry</strong> can provide valuable information about molecular weight, molecular size, branching, and aggregation.</p><p>These techniques are especially powerful when combined with size-exclusion chromatography and can provide information that is not based solely on column elution behavior.</p><p>For a more detailed discussion of detector selection, see the KNAUER blog article <i>“The way you look at it – A comprehensive guide for selecting the appropriate HPLC detector.”</i></p><p>Choosing the detection technology based on analyte properties, required sensitivity, chromatographic conditions, and the type of information needed is essential for obtaining reliable and meaningful HPLC results.</p><h3><strong>Final Thoughts</strong></h3><p>Understanding the desired characteristics and key performance parameters of HPLC detectors helps explain why different detector technologies are suited to different applications.</p><p>No single detector can provide maximum sensitivity, selectivity, universality, robustness, flexibility, and low operating cost simultaneously. Recognizing these trade-offs provides a practical framework for selecting the most appropriate detector for a given chromatographic method.</p><p>Parameters such as data sampling rate, baseline noise, drift, dynamic range, and compatibility with chromatographic conditions are just as important as the underlying detection principle itself.</p><p>In the next articles of this series, we will take a closer look at the individual HPLC detection technologies, beginning with the most widely used optical detection method: <strong>UV/VIS detection</strong>.</p>

Seeing is Believing – Detection Made Easy Part 2: Detector Characteristics

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 17th August 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you posters by <strong>Agilent Technologies / ASMS, Thermo Fisher Scientific / ASMS </strong>and<strong> Shimadzu / ASMS </strong>and application note by<strong> Waters Corporation!</strong></p><h3><strong>1. Agilent Technologies / ASMS: Quantitation of N-Nitroso Duloxetine N-Nitrosamine Drug Substance Related Impurities (NDSRI) in API and Formulation by 6475 LC/TQ</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_drug_impurities_lc_tq_tp_402_asms_2026_en_agilent_b933b9bddf.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Nitrosamine impurities are nitroso derivatives where the nitroso group is attached to an amine. The active pharmaceutical ingredient (API) derived from a class of nitrosamines are known as N-nitrosamine drugsubstance-related impurities (NDSRI). Nitrosamine impurities in APIs and drug products pose safety concerns, even in small quantities, and thus are a major concern for drug makers [1].&nbsp;</p><p>Analytical methods must be sufficiently sensitive to quantify the nitrosamine of interest at levels as low as 10% of the specification limit and, where feasible, down to ≤5% of the specification limit, for the determination of the limit of detection. LC/MS/MS is an inherently selective and highly sensitive analytical technique that is well suited for the identification and quantification of trace-level impurities at extremely low concentrations. Consequently, LC/MS/MS has been widely adopted within the pharmaceutical industry, particularly for nitrosamine analysis [2-3].&nbsp;</p><p>Therefore, manufacturers must develop and qualify a dedicated analytical method that is appropriate for the specific sample matrix and targeted NDSRIs. An LC/MS/MS method based on multiple reaction monitoring (MRM) was developed for the detection and quantification of the N-nitroso duloxetine NDSRI impurity over a concentration range of 0.5 to 40 ppb (absolute standard concentration), using an atmospheric pressure chemical ionization (APCI) source on an Agilent 6475A LC/TQ instrument.</p><h4><strong>Experimental</strong></h4><ul><li><strong>MS:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6475"><strong>6475 Triple Quadrupole LC/MS System</strong></a></li><li><strong>Column:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=Poroshell+HPH+C18"><strong>Poroshell HPH C18</strong></a> (4.6*150mm, 2.7µm)</li></ul><h4><strong>Conclusions</strong></h4><ul><li>A highly sensitive and robust MRM LC/MS/MS method was developed for the quantitation of N-nitroso duloxetine in duloxetine hydrochloride API and finished formulation using the Agilent 6475A LC/TQ.&nbsp;</li><li>The optimized chromatographic conditions provided effective separation of the analyte from the API, placebo, and formulation matrices, ensuring reliable quantitation.&nbsp;</li><li>The calibration curve was linear over the concentration range of 0.5-40 ng/mL, with 1/x weighting. R and R2 values were observed for both to be greater than 0.99.&nbsp;</li><li>Recovery studies demonstrated efficient sample extraction, with recoveries ranging from 92-99% in API samples and 109-116% in pellet formulations at the LOQ, specification, and 200% specification levels.&nbsp;</li><li>The method also showed excellent reproducibility across all evaluated concentration levels, confirming its suitability for routine analysis.</li></ul><h3><strong>2. Shimadzu / ASMS: Non-Targeted Analysis of Per- and Polyfluoroalkyl Substances in Environmental Extracts by Microflow LC-QToF Coupled with Multi-spray ESI</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/TP_562_Non_Targeted_Analysis_of_Per_and_Polyfluoroalkyl_Substances_in_Environmental_Extracts_by_Microflow_LC_Q_To_F_Coupled_with_Multi_spray_ESI_c2a7e1d5bf.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Targeted screening methods are well established for monitoring Perand polyfluoroalkyl substances (PFAS) in environmental samples. However, PFAS comprise a large and evolving class of compounds, creating a need to screen existing, emerging, and transformation products across diverse matrices. Non-targeted Analysis (NTA) allows for the confirmation of suspected PFAS in the environment, but the sample characterization is often limited by analytical sensitivity. Microflow (0.5 – 40 µL/min) coupled with a multi-nozzle emitter enhances sensitivity by improving desolvation. When this is coupled to a high mass accuracy and resolution Quadrupole Time-ofFlight Mass Spectrometer (QToF), capable of data-dependent and independent MS/MS, PFAS characterization of unknown analytes is possible. In this study, various environmental extracts were processed and screened using NTA for PFAS compounds.</p><h4><strong>Methods</strong></h4><p>A <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=Nexera+Mikros"><strong>Shimadzu Nexera Mikros LC</strong> </a>was used to introduce the sample to a Newomics DuoESI source with an M3 emitter on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=9050"><strong>LCMS-9050 QToF MS</strong></a> (Fig. 1). A direct injection on an octadecyl column without delay column was performed. Direct injection was chosen over trapand-elute configuration to minimize selectivity bias caused by the trap column.&nbsp;</p><h4><strong>Conclusion</strong></h4><p>With the increased sensitivity of microflow LC-QToF coupled with multi-spray ESI with DIA acquisition, Insight Explore aided in NTA PFAS analysis. When paired with Insight Discovery and Profiler, a streamlined batch data processing workflow with statistical analysis was shown to effectively handle complex environmental matrices and their analyses.</p><h3><strong>3. Thermo Fisher Scientific / ASMS: Robust and sensitive quantitation of 18 steroids in human serum using Thermo Scientific TSQ Certis Triple Quadrupole mass spectrometer</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004707_lsms_18_steroids_tsq_certis_clinical_po004707_asms2026_na_en_244c52437b.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Accurate quantification of endogenous steroids in human serum is essential for biomonitoring physiological functions such as hormone regulation, reproduction, and immune response in clinical research laboratories. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) is the gold standard for steroid analysis due to its superior sensitivity, selectivity, and multiplexing capability compared to immunoassays. However, steroid measurements remain challenging because of low endogenous concentrations and complex serum matrices. Here, we describe a robust and sensitive LC–MS/MS method for the simultaneous quantification of 18 steroids in human serum using the Thermo Scientific TSQ Certis triple quadrupole mass spectrometer (Figure 1). The method leverages advanced ion source design and fast acquisition to deliver enhanced sensitivity, stability and throughput suitable for high-volume laboratory workflows.</p><h4><strong>Materials and methods</strong></h4><h5><strong>Liquid chromatograph – mass spectrometry&nbsp;</strong></h5><p>Samples were analyzed on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744"><strong>Thermo Scientific Vanquish Horizon UHPLC system</strong></a> coupled to the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2207"><strong>TSQ Certis MS</strong></a><strong> </strong>operated in selected reaction monitoring (SRM) mode. The mobile phase composition, analytical column details, LC gradient, and overlaid extracted ion chromatograms (EICs) are shown in Figure 3.&nbsp;</p><h5><strong>Data analysis&nbsp;</strong></h5><p>Data were acquired and processed using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77"><strong>Thermo Scientific TraceFinder software</strong></a> (v 5.2).</p><h4><strong>Conclusions&nbsp;</strong></h4><p>A sensitive and robust LC–MS/MS method was developed for simultaneous quantification of 18 steroids in human serum using the Vanquish Horizon UHPLC and TSQ Certis MS with OptaMax Plus ion source. The method demonstrated excellent linearity, accuracy, and reproducibility across a wide dynamic range. Optimization of MS conditions, particularly vaporizer temperature, significantly improved sensitivity. Overall, the workflow delivers high sensitivity, selectivity, and long-term stability, providing a reliable solution for high-throughput steroid analysis in clinical and research settings.</p><h3><strong>4. Waters Corporation: Alkaline-hydrolysis Method for PFAS in Textiles and Textiles Products by Xevo™ TQ-S micro with ACQUITY™ UPLC™ H-Class Plus System</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009443_en_0ab4914d72.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><h5><strong>Benefits&nbsp;</strong></h5><ul><li>Alkaline hydrolysis method (EN 17681-1:2025) used for preparing textiles, coated textiles and textile products for PFAS analysis allowed the inclusion of side-chain fluorinated polymers&nbsp;</li><li>Extraction method provided significant advantages in handling complex textile matrices by creating clear extracts free from dispersions, settleable solids and particulates&nbsp;</li><li>Protected the high-value LC-MS/MS components from potential damage&nbsp;</li><li>The method improved analytical sensitivity, reproducibility and accuracy, enabled cost-effective and timeefficient PFAS analysis maintained high analytical quality even for challenging high-matrix samples</li></ul><p>Many PFAS are linked to health concerns because of their ability to interfere with the body’s hormone systems, acting as endocrine disruptors also, linked to other toxic effects. Since textiles come into direct and prolonged contact with the skin, there is an increased risk of human exposure—especially for babies and children.4 In addition, PFAS pose significant environmental concerns. These “forever chemicals” do not readily break down and can be released during manufacturing, washing and disposal, allowing them to enter water pathways and persist in the environment. As a result, testing for PFAS has become increasingly important in the textile industry to demonstrate supply chain transparency, build consumer trust and protect brand reputation.</p><p>Synthetic fluorinated chemicals are categorized into two main groups - non-polymeric PFAS and polymeric PFAS, known for their use as water and grease repellent, heat resistant finishes in textiles, upholstery, leather, apparel, carpet and other industrial applications such as packaging, firefighting foams, non-stick cookware, etc. (Figure 1). PFAS are fluorinated substances contain at least one fully fluorinated methyl or methylene carbon atom (without any H/Cl/Br/I atom attached to it), i.e. with a few noted exceptions, any chemical with at least a perfluorinated methyl group or a perfluorinated methylene group.2 PFAS are classified as long chain and short chain based on their perfluorinated carbon chain length. Short chain fluorinated polymers encompasses a diverse range of chemistries, categorized according to their generic chemical and repeating units structures. Few examples are: derivatives of fluorotelomers, perfluoropolyethers with varying carbon chain lengths and acrylates, epoxylates, urethane, silicones of repeated units based structures.3 Polyfluoroalkyl substances such as fluorotelomer alcohols (FTOHs) contain C–H bonds, rendering them more susceptible to degradation. The ethoxylates in fluorotelomers-methacrylate break down to FTOH whereas polymeric fluorinated urethanes, acrylates break down to PFCAs (non-polymeric and polymeric side chain fluorinated polymers). In contrast, perfluoroalkyl substances such as PFOS and PFOA are extremely persistent due to their fully fluorinated carbon chains.&nbsp;</p><p>In alkaline hydrolysis methods, methanolic aqueous sodium hydroxide is used to break down precursor PFAS such as FTOHs, branched side chain PFAS. This process releases terminal perfluorinated carboxylic acids (PFCAs), which can then be measured. However, the efficiency of hydrolysis varies among different PFAS compounds and the presence of interfering substances such as organic matter or other chemicals in the sample matrix can inhibit the cleavage and detection of these analytes.</p><h4><strong>Experimental</strong></h4><ul><li><strong>LC system:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/198"><strong>ACQUITY UPLC H-Class Plus System</strong></a> and SMFTN fitted with PFAS kit</li><li><strong>Column(s):</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1823"><strong>XBridge™ BEH™ C18 5 µm Column</strong></a>, 4.6 x 100 mm&nbsp;</li><li><strong>MS system: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/209"><strong>Xevo TQ-S micro Mass Spectrometer</strong></a></li></ul><h4><strong>Conclusion&nbsp;</strong></h4><p>By strictly following the critical steps of the alkaline hydrolysis method using methanolic aqueous sodium hydroxide (NaOH) both freely extractable PFAS and those previously bound within side-chain fluorinated polymers such as esters linked to fluorotelomer alcohols (n:2 FTOHs, where n = 4, 6, 8, 10, 12), can be effectively extracted. This approach also safeguards the LC-MS/MS instrumentation from blockages, damage, or contamination by preventing polymer accumulation and matrix interferences.&nbsp;</p><p>The analytical method using the ACQUITY UPLC H-Class Plus System and Xevo TQ-S micro LC-MS/MS System was found to be sensitive, reproducible and accurate, enabling cost-effective and time-efficient PFAS analysis in chemically processed textiles, coated textiles and textile products whilst maintaining high analytical quality even for challenging high-matrix samples.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 34, 2026

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A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Watrex xTrovert XT Automatic DNA/RNA extractor

Watrex xTrovert XT is an automatic DNA/RNA extractor. It streamlines the extraction process, making it more efficient and reproducible.

<p><strong>Automatic extractor utilises a simple principle of two pipetting heads serving a 96-well plate.</strong></p><p>Thanks to easy replacement head options of pipetting with <strong>300 μl tips</strong> or <strong>1000 μl</strong> tips are available. <strong>Automatic optical sensor calibration</strong> enables the use of pipetting tips and <strong>96-well plates</strong> from virtually any supplier. The device is equipped with two magnetic arms for protocols including paramagnetic SPR beads. It also allows for thermal incubation in the range of <strong>25 to 85 °C</strong>. In addition to internal <strong>LED lighting</strong>, the station is equipped with an integrated <strong>UV-C sterilisation unit</strong>. Intuitive control from <strong>TFT touch panel</strong> user interface facilitates easy set-up. xTrovert™ XT is a <strong>fully programmable open system</strong> where user can create their own workflow scripts downloadable as text files from USB flas disk.</p><h3><strong>APPLICATIONS</strong></h3><ul><li><strong>Sample prep for PCR testing</strong></li><li><strong>NGS library prep</strong></li><li><strong>Sanger DNA sequencing</strong></li></ul><h3><strong>CURRENT PROTOCOLS</strong></h3><ul><li><strong>DNA/RNA extraction</strong></li><li><strong>DNA purification</strong></li><li><strong>PCR clean-up/desalting</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Watrex_x_Trovert_XT_Automatic_DNA_RNA_extractor_Compatible_with_any_magnetic_bead_based_kit_23886eea52.jpg" alt="Watrex: Watrex xTrovert XT Automatic DNA/RNA extractor - Compatible with any magnetic bead based kit"></p><h3><strong>TECHNICAL SPECIFICATION</strong></h3><ul><li><strong>Capacity:</strong> 16 or 32 samples/run</li><li><strong>Volume range:</strong> 10 – 600 μl</li><li><strong>Temperature:</strong> 25 – 85 °C</li><li><strong>Sterilization:</strong> UV-C</li><li><strong>Display:</strong> 3,5“ TFT touch display</li><li><strong>Connectivity:</strong> USB 1.0/2.0</li><li><strong>Power source:</strong> 24 V adaptor</li><li><strong>Dimensions in cm:</strong> 65(w) x 65(h) x 34(d)</li><li><strong>Weight:</strong> 25 kg</li></ul><h3><strong>Ordering information:</strong></h3><h5><strong>PN/Component:</strong></h5><ul><li><strong>XTR000-001</strong> xTrovert™ XT Universal robotic pipettor for DNA/RNA extraction &amp; handling</li><li><strong>XTR000-003</strong> Replaceable 8-channel 1000uL pipetting head for xTrovert™ XT</li><li><strong>XTR000-004</strong> Replaceable 8-channel 300uL pipetting head for xTrovert™ XT</li><li><strong>XTR000-100</strong> Deepwell plates (empty) for xTrovert™ XT</li><li><strong>XTR000-220</strong> DNA/RNA extraction kit, mgn. beads, 16-rxns per plate</li></ul><p>The entire system was designed and developed by Watrex Praha and is <strong>manufactured in the Czech Republic</strong> and promises a shift in the performance and efficiency of laboratory processes worldwide!</p><p><strong>You can also visit this product on the </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/laboratory-automation/xtrovert-xt/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Watrex website</strong></a><strong>.</strong></p>

Waters Andrew+ pipetting robot

Andrew+ pipetting robot offers fully automated liquid handling using electronic pipettes and labware holders called Dominos.

<div data-id="7b8f701" data-element_type="widget" data-widget_type="heading.default"><div><h3><strong>Discover lab automation within reach</strong></h3></div></div><div data-id="c1b56fb" data-element_type="widget" data-widget_type="text-editor.default"><div><p><strong>Andrew+ pipetting robot offers fully automated liquid handling using electronic pipettes and labware holders called Dominos. By adding Device+, our ever-expanding portfolio of connected devices and tools for processes such as heating, shaking or labware transportation, Andrew+ evolves into a full walk-away automation solution.</strong></p><p>Creating the connected laboratory, Andrew+ is part of the&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.andrewalliance.com/laboratory-software/"><strong>OneLab</strong></a>&nbsp;ecosystem, executing laboratory protocols designed in OneLab.&nbsp; This flexible architecture enables you to effortlessly transition from laborious manual pipetting procedures to error-free, fully-robotized lab workflows – without any knowledge of programming, laboratory robotics or automation engineering.</p><blockquote><p class="cke-user_style-important"><strong>Video: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=IGu3YxkkZY0"><strong>Andrew+ Demo</strong></a></p></blockquote><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Andrew_pipetting_robot_4_0569f8609e.jpg" alt="Waters Andrew+ pipetting robot" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Andrew_pipetting_robot_4_0569f8609e.jpg 245w," sizes="100vw" width="1440" height="540"></p><h3><strong>Andrew+ Case Studies&nbsp;</strong></h3><blockquote><p class="cke-user_style-important"><a target="_blank" rel="noopener noreferrer" href="https://www.andrewalliance.com/category/case-studies/"><span>See all case studies</span>&nbsp;</a></p></blockquote><div data-id="ee37b9e" data-element_type="widget" data-widget_type="heading.default"><div><h3><strong>Watch Andrew+ in action</strong></h3></div></div><div data-id="b265a33" data-element_type="widget" data-widget_type="text-editor.default"><div><p>In these videos, our biologists explain how Andrew+ enables users to automate commonly used, laborious, liquid handling for PCR Prep and Plasmid DNA Purification, respectively, whilst ensuring superior repeatability and full traceability.</p><blockquote><p class="cke-user_style-important"><strong>Video: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=RnxDri3Z7f4"><strong>PCR Prep</strong></a></p><p class="cke-user_style-important"><strong>Video: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=oWOkzqF9X9I"><strong>Plasmid DNA Prep</strong></a></p></blockquote><div data-id="fc9cb9e" data-element_type="column"><div><div data-id="cbb1a9b" data-element_type="widget" data-widget_type="heading.default"><div><h3><strong>Intuitive user interface</strong></h3></div></div><div data-id="3799119" data-element_type="widget" data-widget_type="text-editor.default"><div><div><div><div><div><div><p>Reproducibility and full traceability of sample preparation is assured using a highly intuitive browser-based software environment.&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.andrewalliance.com/laboratory-software/"><strong>OneLab</strong></a> enables scientists to graphically design their own pipetting protocols in minutes and execute them straight away in any laboratory in the world, even allowing the remote monitoring of ongoing experiments. Use a flexible and secure platform that withstands the rigors of regulatory validation.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Onelab_design_2_489d32ac68/Waters-Onelab-design-2_l.webp" alt="Waters-Onelab-design-2_l.webp" width="1027" height="488"></p><div data-id="9199ea6" data-element_type="column"><div><div data-id="6db85f8" data-element_type="widget" data-widget_type="heading.default"><div><h3><strong>Widest pipetting volume range</strong></h3></div></div><div data-id="ae0caea" data-element_type="widget" data-widget_type="text-editor.default"><div><p>Andrew+ pipetting robot uses single and multichannel electronic pipettes, ensuring the best liquid handling performance and maximum flexibility. Smart electronic pipettes are manufactured by Sartorius and based upon their acclaimed Picus pipettes.</p><p>Electronic pipettes are optimized for used with the Andrew+ pipetting robot, allowing greater flexibility in the type of liquid handling experiments that can be run, providing superior experiment reproducibility, pipetting speed and a market leading dynamic range of dispensing volumes for a liquid handing robot –from 0.2 µL up to 10 mL.</p><figure class="image"><img style="aspect-ratio:529/382;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Andrew_pipetting_robot_f5ca4f825d.jpg" alt="Waters Andrew+ pipetting robot.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Andrew_pipetting_robot_f5ca4f825d.jpg 216w," sizes="100vw" width="529" height="382"></figure><div data-id="ed235f6" data-element_type="column"><div><div data-id="ef6b933" data-element_type="widget" data-widget_type="heading.default"><div><h3><strong>Andrew+ is compact</strong></h3></div></div><div data-id="7537e2e" data-element_type="widget" data-widget_type="text-editor.default"><div><div><div><div><div><div><p>The liquid handling robot is designed to fit the majority of laboratory benches and biosafety cabinets (BSC). Andrew+, even with 2 full rows of Dominos&nbsp;<i>(</i>allowing to use 7 microplates or 56 falcon tubes or 168 microtubes) occupies a depth of ~55cm/22”, that fits even the smallest hoods. The unique compact size and design of our liquid handler allows it to work at a temperature of 20°C-37°C.&nbsp;</p><figure class="image"><img style="aspect-ratio:836/556;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Andrew_pipetting_robot_2_fb174b1453.jpg" alt="Waters Andrew+ pipetting robot - 2.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Andrew_pipetting_robot_2_fb174b1453.jpg 235w," sizes="100vw" width="836" height="556"></figure><div data-id="c9b37a7" data-element_type="widget" data-widget_type="heading.default"><div><h3><strong>Versatile working deck, tools and devices for all your needs</strong></h3></div></div><section data-id="b802f90" data-element_type="section" data-settings="{&quot;jet_parallax_layout_list&quot;:[]}"><div><div data-id="3e689f0" data-element_type="column"><div><div data-id="5dc9372" data-element_type="widget" data-widget_type="text-editor.default"><div><p>The Andrew+ deck layout is quickly adaptable to your experiment materials and are incredibly easy to set with the ever-expanding range of Dominos: a magnetic-based modular solution that enables Andrew+ to use a broad range of consumables.</p></div></div></div></div><div data-id="5626074" data-element_type="column"><div><div data-id="40c6ef1" data-element_type="widget" data-widget_type="text-editor.default"><div><p>In addition to pipetting liquids, the Andrew+ liquid handler can perform a wide range of complex experimental steps such as tube/plate transport or solid-phase extraction using vacuum.<br>Thanks to OneLab, you will benefit to a seamless integration of new capabilities added to your setting.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Andrew_pipetting_robot_3_89b87a0d2e.jpg" alt="Waters Andrew+ pipetting robot - 3.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Andrew_pipetting_robot_3_89b87a0d2e.jpg 245w," sizes="100vw" width="1734" height="598"></p><div data-id="d090821" data-element_type="column"><div><div data-id="f9d733b" data-element_type="widget" data-widget_type="heading.default"><div><h3><strong>Automated Protocols with Andrew+</strong></h3><p>Specifically optimized for Andrew+, various applications and methods guarantee the delivery of better pipetting accuracy and improved reproducibility.</p><blockquote><ul><li><div><a target="_blank" rel="noopener noreferrer" href="https://onelab.andrewalliance.com/library/sars-cov-2-lc-ms-kit-ruo-9lwrvwkA"><span>Automated preparation of SARS-CoV-2 peptides for LC-MS analysis</span></a></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://onelab.andrewalliance.com/library/automated-antibody-purification-jlwKQ0aY"><span>Automated Antibody Purification for higher recovery and reproducibility</span></a></div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://onelab.andrewalliance.com/app/lab/bl6MQP6z/library/standard-curve-generation-KlwRbwR3"><span>Reliable and Robust Sample Prep for Standard Curve Generation</span></a></div></li></ul><p class="cke-user_style-important"><a target="_blank" rel="noopener noreferrer" href="https://onelab.andrewalliance.com/library"><span><strong>More to discover in the Protocol Library</strong></span></a></p></blockquote><h3><strong>Get an extra pair of hands</strong></h3><div data-id="aa4715f" data-element_type="widget" data-widget_type="heading.default"><div><h4><strong>Reproducibility</strong></h4></div></div><div data-id="a1d2f3b" data-element_type="widget" data-widget_type="text-editor.default"><div><div><div data-animationtype="fadeInDown" data-animationduration="0.7" data-animationoffset="top-into-view"><div><p>Laboratory automation eliminates most human errors resulting in more reliable data and improved productivity. Together with complete integration inside OneLab, all experimental data from Andrew+ will be recorded and stored, improving the traceability of experiments and the data analysis.</p><div data-id="fb36639" data-element_type="widget" data-widget_type="heading.default"><div><h4><strong>Productivity</strong></h4></div></div><div data-id="c5f6abd" data-element_type="widget" data-widget_type="text-editor.default"><div><div><div data-animationtype="fadeInDown" data-animationduration="0.7" data-animationoffset="top-into-view"><div><div><div data-animationtype="fadeInDown" data-animationduration="0.7" data-animationoffset="top-into-view"><div><p>Andrew+ combines the flexibility of single channel pipetting (0.2 μL to 10 mL) with the higher throughput of multichannel pipetting. In addition, Andrew+ reduces the need to repeat experiments due to pipetting errors.</p><div data-id="0a035d7" data-element_type="widget" data-widget_type="heading.default"><div><h4><strong>Promoting health</strong></h4></div></div><div data-id="a72e364" data-element_type="widget" data-widget_type="text-editor.default"><div><div><div data-animationtype="fadeInDown" data-animationduration="0.7" data-animationoffset="top-into-view"><div><div id="applications"><div><div><div><div><div id="icon-area" data-animationoffset="top-into-view"><div><div data-animationtype="fadeInDown" data-animationduration="0.7" data-animationoffset="top-into-view"><div><p>Pipetting requires repetitive movements (of up to 500 times per day for the average user) that can lead to musculo-skeletal disorders like repetitive strain injury. Andrew+ helps eliminates physical stress to professionals, and also reduces the risk of exposure to hazardous materials used in certain experimental procedures.</p></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></section></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

Waters Otto SPEcialist

Controlled by an interfaced tablet, the easy-to-use Otto software simplifies solid-phase extraction method development and transfer, positive pressure optimization, and real-time manipulation of pressure profiles to further improve method robustness.

<div><div><h4><strong>Consistent, reproducible solid-phase extraction sample preparation &nbsp;</strong></h4></div></div><div><div><p><strong>Improving the reproducibility, method transfer, and processing efficiency of solid-phase extraction (SPE) sample preparation is challenging, and addressing it starts with your solutions. Achieve high-quality sample preparation and extraction by reducing user variability with the programmed pressure profiles and methods on the Otto SPEcialist Positive Pressure Manifold.</strong></p><p>Controlled by an interfaced tablet, the easy-to-use Otto software simplifies <strong>solid-phase extraction</strong> method development and transfer, positive pressure optimization, and real-time manipulation of pressure profiles to further improve method robustness. The <strong>Otto SPEcialist Positive Pressure Manifold</strong> is a semi-automated system designed for use with both SPE plates and cartridges, including µElution and macro elution plates and 1 cc, 3 cc, and 6 cc cartridges.</p><div><div><div><div><ul><li><strong>Improve reproducibility</strong> and method development for SPE sample preparation</li><li><strong>Achieve consistent performance</strong> across SPE runs by eliminating technique variation due to differing levels of user expertise</li><li><strong>Accommodate varying SPE processing needs</strong> from very low to high flow rates for sorbent bed drying</li><li>Ensure novice and expert SPE users can get up and running quickly with <strong>easy-to-use software</strong> that requires little to no training</li><li><strong>Perform multiple workflows</strong> in a single day</li><li>Process a wide range of sample volumes using <strong>micro and macro elution plates</strong>; and <strong>1 cc, 3 cc, and 6 cc cartridges</strong></li><li><strong>Compact device</strong> that fits easily on a bench or under a hood</li></ul></div></div><div><div><h5><strong>Recommended Use</strong></h5><p>To improve reproducibility and analytical consistency during <strong>sample preparation and solid-phase extraction</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Otto_SP_Ecialist_4_66c2f095a0.jpg" alt="Waters Otto SPEcialist: The Otto Specialist Positive Pressure Manifold is designed to improve analytical consistency during sample preparation and solid-phase extraction. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Otto_SP_Ecialist_4_66c2f095a0.jpg 234w," sizes="100vw" width="1024" height="682"></p><div><div><h3><strong>Optimize solid-phase extraction workflow efficiency</strong></h3><p>The <strong>Otto SPEcialist Positive Pressure Manifold</strong> is controlled by the easy-to-use <strong>Otto Software</strong>. The <strong>touchscreen interface</strong> requires little-to-no training for novice and expert SPE users. Easily create pressure profiles with optimized flow rates for each step of your process. Whether it’s a simple “load, wash, elute” or a more complicated workflow with multiple steps, <strong>Otto Software</strong> makes running programed methods simple.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Otto_SP_Ecialist_ade08ca842.jpg" alt="Waters Otto SPEcialist: Pressure profile example created in the Otto SPEcialist software designed to increase pressure over time. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Otto_SP_Ecialist_ade08ca842.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div><div><div><h3><strong>Ensure consistent SPE</strong></h3><p>Running programmed methods, including a combination of pressure profiles and manifold commands, eliminates user-to-user variability ensuring consistency across users and labs. <strong>Otto Software</strong> tracks and documents each pressure profile and method processed, ensuring workflow consistency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Otto_SP_Ecialist_2_9dc31e4ffc.jpg" alt="Waters Otto SPEcialist: Method set-up on an Otto SPEcialist System incorporating opening and closing of the manifold, pausing the method, and running a pressure profile. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Otto_SP_Ecialist_2_9dc31e4ffc.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div><div><div><h3><strong>Reproducible, high-quality results</strong></h3><p><strong>Reproducibility</strong> is especially important when generating <strong>calibration curves</strong>. With its <strong>programmable pressure profiles</strong> and methods, <strong>Otto SPEcialist Positive Pressure Manifold</strong> performs consistently across all SPE runs, independent of user expertise.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Otto_SP_Ecialist_3_adeb0ee489.jpg" alt="Waters Otto SPEcialist: Calibration curves for sulfadimethoxine generated on a vacuum manifold and Otto SPEcialist by a novice user. Replicates (n=3) of each level of the curve were run and standard deviations were determined and are indicated error bars. The Otto SPEcialist error bars, while present are very small and difficult to discern. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Otto_SP_Ecialist_3_adeb0ee489.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div></div></div></div></div></div></div>

Watrex Streamline FC1 Fraction Collector

The fully automated fraction collector FC1 offers a universal solution for a number of applications requiring the collection of fractions, e.g. protein purification, preparative chromatography.

<p><strong>The fully automated fraction collector FC1 offers a universal solution for a number of applications requiring the collection of fractions, e.g. protein purification, preparative chromatography or other methods for obtaining samples by fractionation.</strong></p><blockquote><p><span style="font-size:18px;"><strong>Price: 4 260</strong><bdi><span class="wmc-cache-pid woocommerce-Price-amount amount" data-wmc_product_id="24487"><strong>&nbsp;</strong></span><span class="wmc-cache-pid woocommerce-Price-amount amount woocommerce-Price-currencySymbol" data-wmc_product_id="24487"><strong>€ ex VAT</strong></span></bdi></span></p></blockquote><p>For advanced programming and connection to chromatography software, the device is equipped with a <strong>USB interface</strong>. FC1 is supported by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/305"><strong>Clarity Chromatography Station</strong></a></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_schema_procesu_sberu_frakci_pomoci_frakcni_kolektor_FC_1_fa579d59f2/Watrex-schema-procesu-sberu-frakci-pomoci-frakcni-kolektor-FC1.png" alt="Watrex: Schematic of the fraction collection process using the FC1 fraction collector"></p><p>The <strong>robust</strong> design of the FC1 ensures reliable X-Y displacement that carries the switching valve. Placing the fraction switch directly above the receiving vessel <strong>minimizes dead volume between fractions</strong>.</p><p>Depending on the <strong>required volume of fractions</strong>, different types of test tube stands are offered with the device. Furthermore, it is also possible to use large-volume containers.</p><p>The operator controls and programs the fraction collector from the <strong>touch screen</strong> on the front panel of the device using intuitively arranged screens with adjustable parameters for a given fraction collection series.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Nastaveni_treshold_limitu_pro_sber_frakci_pomoci_sberace_frakci_FC_1_f48d645dfc/Watrex-Nastaveni-treshold-limitu-pro-sber-frakci-pomoci-sberace-frakci-FC1.jpg" alt="Watrex: Setting the treshold limit for fraction collection using the FC1 fraction collector"></p><h3><strong>FEATURES</strong></h3><ul><li>Simple operation</li><li>Color touch panel control</li><li>Optional analog input</li><li>Selection of rack types</li><li>Clarity CDS supported</li></ul><h3><strong>TECHNICAL SPECIFICATION</strong></h3><ul><li><strong>Max. flow:</strong> 50 ml/min</li><li><strong>Wetted Materials:</strong> PTFE, PEEK</li><li><strong>User control:</strong> Color touch panel</li><li><strong>PC connection:</strong> USB</li><li><strong>External control:</strong> TTL (contact closure) - RUN, STOP, NEXT, COLLECT</li><li><strong>Power supply:</strong> 24V DC external power supply</li></ul><h3><strong>Ordering information:</strong></h3><h5><strong>PN/Component:</strong></h5><ul><li><strong>SFC101-001</strong> Streamline™ FC1 Fraction Collector</li><li><strong>SFC101-002</strong> Cooling option for FC1 Fraction Collector</li><li><strong>SFC101-003</strong> Analog Signal Monitoring, Input for FC1 Fraction Collector</li></ul><p><strong>You can also visit this product on the </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/dedicated-instruments/streamline-fc1-fraction-collector/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Watrex website</strong></a><strong>.</strong></p>

Thermo Scientific Dionex AutoTrace 280 Solid-Phase Extraction Instrument

Extract analytes of interest from large volume aqueous samples easily and efficiently with the Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction (SPE) Instrument.

<p><strong>Extract analytes of interest from large volume aqueous samples easily and efficiently with the Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction (SPE) Instrument.&nbsp;</strong></p><p>The AutoTrace 280 automates all four steps of SPE (conditioning, loading, rinsing, and eluting), reducing solvent consumption and improving recovery and reproducibility. Boost productivity and reduce cost of analysis by simultaneously processing up to six samples in 2-3 hours with only 15 minutes of operator involvement. The AutoTrace 280 meets requirements in US EPA methods including the 500 and 600 series.</p><h5><strong>Improved reliability and recovery</strong></h5><ul><li>Automated SPE minimizes operator intervention</li><li>Positive pressure loading and elution of samples and solvents provide constant flow for improved analytical precision</li></ul><h5><strong>Flexibility in operation</strong></h5><ul><li>Loads sample volumes from 20 mL to 4 L</li><li>Compatible with 1, 3, and 6 mL cartridges and with 47 mm SPE disks</li><li>Automatically conditions, rinses, and elutes SPE cartridges with a choice of five solvents</li><li>Offers a choice of six different collection vial racks</li></ul><h5><strong>Improved laboratory safety</strong></h5><ul><li>Minimal sample handling reduces operator exposure to hazardous chemicals</li><li>No hood required</li></ul><h5><strong>Enhanced laboratory productivity and reduced costs</strong></h5><ul><li>Unattended operation</li><li>Batch processing of up to 6 samples</li><li>Reduced solvent usage</li><li>Segregates aqueous and solvent waste using separate waste lines for efficient, cost-effective waste handling</li></ul><h5><strong>Supports a range of matrices and applications</strong></h5><ul><li>Ability to process samples containing suspended solids</li><li>Meets requirements for solid-phase extraction for U.S. EPA methods including 500 and 600 series</li></ul><h5><strong>Recommended use</strong></h5><ul><li>Analyze prepared samples with Thermo Scientific LC or GC systems for a fully integrated workflow</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Instrument Type:</strong> Automated Solid Phase Extraction (SPE) System</li><li><strong>Application:</strong> For use with 47 mm SPE disks</li><li><strong>Model Description:</strong> AutoTrace 280 SPE System</li></ul><h5><strong>Performance &amp; Operating Details</strong></h5><ul><li><strong>Disk Compatibility:</strong> Designed for 47 mm SPE disks</li><li><strong>Pressure Range:</strong><ul><li>Output: 0 to 30 psi (0 to 1.4 bar)</li><li>Input: Up to 100 psi (6.9 bar) maximum</li></ul></li><li><strong>Voltage:</strong> 100–240 V ±10%</li><li><strong>Frequency:</strong> 47–63 Hz</li></ul><h5><strong>Physical Specifications</strong></h5><ul><li><strong>Dimensions (D × W × H):</strong> 57 × 63.5 × 69 cm</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific EXTREVA ASE Accelerated Solvent Extractor

Achieve true walkaway sample-to-vial sample preparation with the Thermo Scientific™ EXTREVA™ ASE™ Accelerated Solvent Extractor. 

<p><strong>Achieve true walkaway sample-to-vial sample preparation with the Thermo Scientific™ EXTREVA™ ASE™ Accelerated Solvent Extractor. Without any intervention, perform sample extraction, in-cell cleanup and evaporation—all in one seamless operation—reducing manual labor from hours to minutes and enabling you to focus on other lab priorities while using up to 50% less solvent than other sample preparation techniques.</strong></p><p><strong>Accelerate your journey from sample to vial</strong></p><h5><strong>The EXTREVA ASE Accelerated Solvent Extractor offers:</strong></h5><ul><li><strong>All-in-one sample preparation.</strong> The EXTREVA ASE system automatically extracts and concentrates samples, eliminating the need to manually move sample extracts to another device for solvent evaporation.</li><li><strong>A walk-away solution. </strong>The system minimizes hands-on sample preparation tasks, enabling staff to accomplish other tasks.</li><li><strong>Parallel extraction. </strong>One EXTREVA ASE system can perform four parallel sample extractions, substantially increasing throughput.</li><li><strong>Gas-assisted extraction. </strong>The dynamic gas-assisted extraction mode reduces solvent consumption to between 5 and 100 milliliters per sample in most cases.</li><li><strong>Automation. </strong>Full automation improves recoveries and reproducibility while reducing training needs. Unlike humans, automated systems are ideal for performing the same tedious task repeatedly, and they don’t make mistakes.</li><li><strong>Method flexibility and optimization</strong>. Up to six different solvents can be used, including wash solvents for cleaning between methods. Precise flow control optimizes methods.</li><li><strong>Barcode-reading.</strong> The system tracks sample information and preparation parameters using a 2-D bar code reader to facilitate tracking and integrity. Data is recorded on the instrument or Thermo Scientific™ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>Chromeleon™ Chromatography Data System (CDS) software</strong></a>.</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Instrument Type:</strong> Automated Sample Preparation System</li><li><strong>Application:</strong> Simultaneous sample extraction and concentration</li><li><strong>Model Description:</strong> EXTREVA ASE Extraction and Evaporation System</li></ul><h5><strong>Performance &amp; Capabilities</strong></h5><ul><li><strong>Extraction Temperature Range:</strong> 40–200 °C</li><li><strong>Evaporation Temperature Range:</strong> 40–100 °C</li><li><strong>Compatible Solvents:</strong> Wide range of organic and aqueous solvents</li><li><strong>Pressure Capability:</strong> Up to 200 psi</li><li><strong>Power Consumption:</strong> 2000 W</li><li><strong>Frequency:</strong> 50/60 Hz</li></ul><h5><strong>Sample &amp; Solvent Handling</strong></h5><ul><li><strong>Extraction Cells (stainless steel):</strong> 1, 5, 10, 22, 34, 66, 100 mL</li><li><strong>Extraction Cell Tray:</strong> 16-position with 1 rinse</li><li><strong>Collection Vials:</strong><ul><li>60 mL and 100 mL evaporation vials</li><li>250 mL evaporation bottles</li><li>Direct concentrate to 2 mL sample vials with rinse capability</li></ul></li><li><strong>Collection Vial Tray:</strong> 16-position tray insert for 60 mL vials and 250 mL bottles; includes 4 rinse positions</li></ul><h5><strong>Utilities &amp; Requirements</strong></h5><ul><li><strong>Pneumatic Requirements:</strong><ul><li>Air: 130–140 psi</li><li>Nitrogen: 350–450 psi</li></ul></li></ul><h5><strong>Physical Specifications</strong></h5><ul><li><strong>Dimensions (D × W × H):</strong> 65.2 × 100 × 88.6 cm (25.6 × 39.4 × 34.8 in.)</li><li><strong>Weight:</strong> 160 kg (353 lb)</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor

Save time, reduce solvent consumption, and boost productivity with Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor.

<div><p><strong>Automate extraction, filtration and clean up of compounds from solid and semisolid samples in minutes using the automated Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor. This system accommodates sample sizes of 1-100g, allows unattended extraction of up to 24 samples and uses 50 to 90% less solvent compared to other methods.&nbsp;</strong></p><p>The chemically inert pathway supports acid and alkaline sample matrices and solvents. Flexible, easy-to-operate and cost-effective, the Dionex ASE 350 is ideally suited for high-throughput laboratories and for a wide variety of applications.</p><div><h4><strong>Rapid and efficient extractions</strong></h4><ul><li>Elevated temperature (up to 200°C) and pressure (1500psi) reduce extraction times and solvent consumption</li><li>Fast pump (70mL/min.) enables fast extractions (typically 12-20 minutes)</li><li>Specialized oven design ensures precise temperature control for excellent reproducibility</li><li>Integrated solvent controller allows mixing and delivery of up to three solvents</li><li>The <strong>Thermo Scientific™ SmartRun™ System</strong> ensures cell size and collection vessel size are matched for an optimal extraction</li></ul><h4><strong>Enhanced flexibility to support multiple applications</strong></h4><ul><li>System accommodates <strong>1, 5, 10, 22, 34, 66 </strong>and <strong>100mL cell sizes</strong></li><li>pH-hardened pathway with <strong>Thermo Scientific™ Dionium™ components and cell</strong> enables compatibility with acid or alkaline pretreated matrices</li></ul><h4><strong>Dramatically reduced solvent consumption</strong></h4><ul><li>Requires less than 50mL solvent for a 20g sample</li><li>Includes solvent saver mode for further solvent savings</li></ul><h4><strong>Enhanced laboratory productivity</strong></h4><ul><li>System holds up to 24 samples for unattended operation</li><li>Flow-through technology allows in-line filtration and in-cell cleanup</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_ASE_350_system_2_9426ff3f9a/Thermo-Scientific-Dionex-ASE-350-system_2.jpg" alt="Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_ASE_350_system_2_9426ff3f9a/thumbnail_Thermo-Scientific-Dionex-ASE-350-system_2.jpg 114w," sizes="100vw" width="579" height="792"></p><h3><strong>🛠️ Specifications</strong></h3><ul><li><strong>Collection Vial Tray</strong>: 26-position tray insert for 60 mL vials; 19-position tray insert for 250 mL bottles</li><li><strong>Depth (English)</strong>: 27.3 in.</li><li><strong>Depth (Metric)</strong>: 69.3 cm</li><li><strong>Description</strong>: Extractor Only</li><li><strong>Extraction Fluids</strong>: Compatible with a wide range of organic and aqueous solvents</li><li><strong>Fluid Sensors</strong>: IR</li><li><strong>Frequency</strong>: 50/60 Hz</li><li><strong>Height (English)</strong>: 24.3 in.</li><li><strong>Height (Metric)</strong>: 61.7 cm</li><li><strong>Pneumatic Requirements</strong>: Air at 400–827 kPa (60–120 psi); N2 (at 1034 ×–1340 kPa (150–200 psi))</li><li><strong>Power Consumption</strong>: 500 Max VA</li><li><strong>Pressure</strong>: 10 MPa (1500 psi)</li><li><strong>Pump Flow</strong>: 70 mL/min</li><li><strong>Temperature Control</strong>: Up to 200 °C</li><li><strong>Weight (English)</strong>: 140 lb.</li><li><strong>Weight (Metric)</strong>: 65 kg</li><li><strong>Width (English)</strong>: 26.5 in.</li><li><strong>Width (Metric)</strong>: 67.3 cm</li><li><strong>Collection Vials</strong>: 60, 250 mL</li><li><strong>Dimensions (D x W x H)</strong>: 69.3 x 67.3 x 61.7 cm</li><li><strong>Extraction Cell Tray</strong>: 24 Cell, 2 Rinse</li><li><strong>Extraction Cells</strong>: 1, 5, 10, 22, 34, 66, 100 mL</li><li><strong>For Use With (Equipment)</strong>: Dionex ASE 350</li><li><strong>Type</strong>: Dionex ASE 350 System</li><li><strong>Unit Size</strong>: Each</li></ul></div></div>

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