<h3>How are PFAS regulations changing, and what role will ion chromatography play in the future of environmental testing?</h3>In this episode, &nbsp;David interviews Jay Gandhi, PhD, Vertical Markets Manager at Metrohm USA, for an in-depth discussion on PFAS analysis, the rise of AOF (Adsorbable Organic Fluorine) testing, and the development of EPA 1621 and ISO 18127 standards.With more than 30 years of experience in ion chromatography, Jay shares valuable perspective on how the field has evolved—from early applications and NASA water recovery projects to today’s challenges involving PFAS, TFA, and emerging contaminants.<h4>Topics covered in this episode include:</h4><ul><li>What PFAS are and why they matter</li><li>The difference between targeted PFAS methods and AOF analysis</li><li>How combustion ion chromatography works</li><li>Behind the scenes of EPA 1621 and ISO 18127 method development</li><li>Regulatory trends in water, textiles, and food packaging</li><li>TFA analysis using IC-MS and IC-MS/MS</li><li>The future of ion chromatography in environmental labs</li></ul><h3>Video Transcription</h3>For more than three decades, Jay Gandhi has worked with ion chromatography (IC), watching the technique expand from a specialized tool for water analysis into an analytical platform used across environmental testing, food and beverage analysis, semiconductor manufacturing, battery applications, and, increasingly, PFAS analysis.In an interview for Concentrating on Chromatography, Gandhi discusses his introduction to IC during his work on a NASA Mars exploration project, the emergence of PFAS as a major analytical challenge, and why techniques such as adsorbable organic fluorine (AOF) determination and combustion ion chromatography (CIC) can provide information that targeted LC-MS analysis alone cannot deliver. He also examines the development of standardized methods, sample preparation challenges, and the future role of IC-MS and total fluorine approaches in PFAS monitoring.<h4>From NASA to ion chromatography</h4>Gandhi's introduction to ion chromatography came through his work as a principal scientist on a NASA Mars exploration project focused on water recovery under zero-gravity conditions. IC played an important role because it enabled researchers to establish the ionic balance of anions and cations in water.The technology was used both to investigate micronutrients relevant to agricultural applications and to evaluate water purity for potential astronaut consumption. During approximately three years of this work, Gandhi gained extensive experience with ion chromatography. This ultimately led him deeper into IC instrumentation and, 24 years ago, to joining Metrohm.At the beginning of his career, IC was strongly associated with relatively straightforward anion and cation analysis in water. The technique itself had been used for water analysis since the 1970s, but Gandhi has seen its application range expand dramatically.Food and beverage testing and environmental analysis became important fields, supported by both direct and indirect detection strategies. More recently, IC has also found applications in areas such as the semiconductor industry and battery recycling.In Gandhi's words, ion chromatography has effectively "bloomed" into numerous application areas.<h4>PFAS changes the analytical challenge</h4>PFAS represent a particularly important chapter in this development. These chemicals have been used for decades in numerous consumer and industrial products, including non-stick materials, firefighting foams, water-resistant textiles and other applications.Gandhi recalls work on PFOA and PFOS using ion chromatography with conductivity detection as early as 2008. However, the achievable detection limits were insufficient for the extremely low concentrations that later became important in PFAS analysis.A major turning point for him came in 2015, when he encountered research from Sweden using combustion ion chromatography to investigate total fluorine. Subsequent collaboration with researchers at Clarkson University and Harvard University, particularly on aqueous film-forming foam (AFFF), further demonstrated the importance of looking beyond individual targeted PFAS compounds.<h4>Why targeted LC-MS does not tell the whole story</h4>Targeted LC-MS is highly sensitive and selective and can provide detailed information about individual PFAS compounds. However, according to Gandhi, the number of possible PFAS structures in the environment is vastly greater than the number routinely included in targeted methods.A targeted method may monitor dozens or perhaps around one hundred compounds, while the overall PFAS universe can include thousands of chemical structures. Consequently, targeted analysis may characterize only a small fraction of the total PFAS burden.There are other practical considerations. LC-MS measurements can be affected by matrix-dependent ion suppression, while accurate quantification frequently relies on isotope-labelled internal standards. Such standards are available for only a limited number of compounds and can be expensive.This is where measurements of total or adsorbable organic fluorine can provide complementary information. Rather than asking which specific PFAS compounds are present?, these approaches can help answer a broader question: how much fluorine associated with this chemical space is present in the sample?<h4>AOF as a complementary approach</h4>Adsorbable organic fluorine analysis provides a different perspective from targeted LC-MS. In the workflow discussed by Gandhi, organic fluorine compounds are captured during sample preparation and subsequently measured using combustion ion chromatography.The principle of CIC builds upon older total organic halogen analysis. The sample is combusted at temperatures above approximately 1,000 °C, breaking carbon-halogen bonds. In PFAS-related analysis, carbon-fluorine bonds are broken and the resulting fluoride is collected in an absorption solution.Ion chromatography is then used to separate and quantify the fluoride.Replacing older detection approaches with IC provides both sensitivity and selectivity for the halogens being measured. Effective combustion is critical: oxygen supports the combustion process, while a carrier gas transports the products into the absorption solution for subsequent IC analysis.<h4>The challenge of inorganic fluoride</h4>The broad response of CIC is simultaneously one of its strengths and one of its analytical challenges. Combustion does not distinguish fluorine originating from PFAS from fluorine present in inorganic compounds such as fluoride salts.This becomes particularly important when analyzing water containing relatively high concentrations of inorganic fluoride while attempting to quantify organic fluorine at very low levels.Sample preparation therefore has a critical role. Inorganic fluoride must be effectively separated from the fraction of interest, while materials used during sample preparation must themselves have sufficiently low fluoride backgrounds.Contamination can come from unexpected sources. Gandhi points out that background fluoride may originate not only from reagents and sample preparation materials but potentially from the laboratory environment. Careful blank control, appropriate materials and rigorous evaluation of interferences are therefore essential when moving toward increasingly low detection limits.<h4>From research method to international standardization</h4>The development of standardized AOF methods illustrates the importance of collaboration between academia, industry, regulatory organizations and analytical laboratories.Gandhi describes work that began in Europe around 2015 and subsequently progressed through German standardization activities, ISO development, ASTM-related collaboration and the U.S. EPA. The process eventually contributed to methods such as EPA Method 1621.He emphasizes that this was not the work of a single scientist or organization. Developing a robust method required years of experiments, discussions and sometimes difficult technical debates among numerous stakeholders.For Gandhi, the essential principle was that experimental evidence ultimately had to determine the outcome. Collaboration becomes possible when scientists focus on the data rather than the organizations they represent.<h4>PFAS monitoring is moving beyond drinking water</h4>Regulatory attention to PFAS is also expanding beyond drinking water. Gandhi describes a broader strategy that begins with reducing the manufacture and use of PFAS-containing materials and continues with removing existing contamination from the environment.Wastewater represents an important part of this picture because PFAS can enter it from numerous sources. Historical contamination from landfills and contaminated sites can also migrate into groundwater and ultimately affect drinking-water supplies.Consumer products are another area of increasing attention. Food packaging, disposable containers, automotive fabrics, outdoor clothing and water-repellent textiles have historically used fluorinated substances to provide properties such as grease, water or stain resistance.Reducing PFAS use in such products could therefore gradually decrease the amount entering waste streams and the wider environment.<h4>Screening first, regulation later?</h4>Gandhi expects AOF to be particularly valuable as a screening tool, providing an estimate of broader fluorine contamination before laboratories proceed to more specific analyses.Its importance becomes clear when AOF results are compared with targeted PFAS measurements. Gandhi reports that laboratories working with EPA Method 1621 have observed differences between targeted PFAS results and broader AOF measurements ranging from approximately 300- to 4,000-fold in the total fluorine mass balance.Such discrepancies suggest that targeted methods can leave a substantial portion of fluorine unexplained.Rather than competing with LC-MS, AOF and CIC therefore provide complementary information. A broad screening method can reveal the magnitude of total contamination, while targeted mass spectrometry identifies and quantifies specific compounds of interest.<h4>Where IC-MS fits into the PFAS toolbox</h4>No single technique can cover the entire PFAS problem. Gandhi highlights very short-chain, highly polar fluorinated compounds as another analytical challenge.Compounds such as trifluoroacetic acid (TFA) are highly water-soluble and may not be quantitatively retained by the carbon materials used for AOF sample preparation. Direct ion chromatography can provide an alternative route for separating such compounds from common inorganic anions.Coupling IC with mass spectrometry adds another level of sensitivity and selectivity. Gandhi therefore sees IC-MS and IC-MS/MS as increasingly important tools for highly polar fluorinated compounds. Similar approaches can also be useful beyond PFAS, including the determination of polar pesticides such as glyphosate and glufosinate.<h4>Toward lower detection limits and more complete PFAS analysis</h4>For Gandhi, current standardized methods represent a beginning rather than an endpoint. Improvements in instrumentation and particularly sample preparation could push total fluorine analysis to substantially lower concentrations.He notes that current combustion IC configurations can reach approximately 0.5 ppb, or 500 parts per trillion, and believes that improvements in sample volume, preparation procedures and mitigation of inorganic fluoride backgrounds could eventually move detection below 100 ppt and potentially toward 50 ppt.Extractable organic fluorine workflows could provide another bridge between broad fluorine screening and targeted LC-MS methods. Combining improved extraction with CIC could allow samples to be screened for organic fluorine before more detailed compound-specific characterization.The broader message from Gandhi's career is that PFAS analysis is unlikely to be solved by a single analytical technique. Targeted LC-MS provides compound-specific sensitivity and selectivity; AOF and combustion IC reveal a broader fluorine picture; and IC-MS can address highly polar compounds that are difficult to capture using conventional workflows. Used together, these techniques can provide laboratories with a much more complete understanding of PFAS contamination than any one approach alone.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

The Future of Ion Chromatography | PFAS Testing, EPA 1621 & AOF Explained

In late February 2025, a train carrying benzene derailed in Hustopeče nad Bečvou, and within hours, a race was on to save the groundwater and surface water. One of the most toxic industrial substances began seeping into the gravel-sand subsoil. While fieldwork was underway to prevent the spread of contamination, decisions were already being made in the CXI TUL laboratories regarding how to subsequently clean up the contaminated rock environment.The accident ranks among the most serious environmental incidents in the Czech Republic in recent years. After a tanker train derailed, hundreds of metric tons of benzene leaked; due to the highly permeable geological subsoil, it quickly seeped into deeper soil layers and into the groundwater. The combination of the substance’s high toxicity and local conditions complicated both the emergency response and the subsequent remediation. The key was to stop further spread, determine the extent of the contamination, and establish a remediation strategy for the entire site.It was at this stage that CXI TUL became involved in the solution. At the request of the renowned remediation company DEKONTA, a.s., a team led by RNDr. Jan Němeček, Ph.D., began testing the feasibility of the in situ chemical oxidation (ISCO) method. This technology is designed to break down benzene directly within the rock matrix, without the need for extensive excavation of contaminated soil or the pumping out of large volumes of groundwater. The goal was simple: to find a method that would work quickly, safely, and under the actual conditions of the affected site.<h4>Benzene Remediation: Technically Simple, Complicated in Practice</h4>Benzene is a highly toxic and carcinogenic substance that rapidly penetrates into groundwater in permeable subsoil, where it dissolves and spreads further as the water flows toward surface water bodies. Benzene also binds to rock particles and migrates in the form of vapors into overlying unconsolidated layers.Scientists in Liberec therefore tested in situ chemical oxidation (ISCO) technology under laboratory conditions. The goal was to find an oxidant capable of breaking down benzene directly underground into less hazardous substances.The tests evaluated two main options: activated hydrogen peroxide and sodium peroxodisulfate. Both substances removed up to 99.99% of the benzene, but there was a difference in their kinetics and impacts on the natural environment. Activated hydrogen peroxide achieved nearly complete degradation of the contaminant within a single day. Activated sodium peroxodisulfate was slower and also significantly altered the chemistry of the water; it acidified the environment and increased the sulfate content in the water, which poses an additional environmental burden.For field applications, the researchers therefore unequivocally recommended activated hydrogen peroxide.<h4>Dosage Matters</h4>It was not just the oxidant’s effectiveness that was key. It was equally important to determine how the chemical reaction would behave in a real geological environment. The team of scientists therefore also analyzed what is known as soil oxidant consumption (SOD). It turned out that a significant portion of the oxidizing agent does not react solely with benzene but is also consumed to a significant extent by the rock environment itself, particularly by natural organic substances present in the fine-grained fraction of the soil.An oxidant consumption value was determined specifically for the local composition of the aquifers, which is essential for the correct dosing of chemicals in the field so that the remediation is not only effective but also economically viable and safe for surrounding ecosystems.<h4>One Year After the Accident: Research Turns into Real-World Assistance</h4>While the public often perceives university science as a distant world of laboratories and grant projects, it is precisely situations like this that demonstrate its practical impact. The results of the work by researchers at CXI TUL did not remain confined to academic studies but directly influenced decisions regarding the technology used to clean up one of the largest environmental accidents in recent years.

Science on the Front Lines: Cleanup of a Benzene Spill

This study uses ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) lipidomics to characterize plasma lipid alterations associated with long-term aromatase inhibitor therapy in postmenopausal breast cancer patients. A total of 649 lipid species across 23 classes and subclasses were relatively quantified, revealing significant differences in phosphatidylcholines, sphingomyelins, ceramides, hexosylceramides, and other lipid groups.Multivariate analysis showed clear group separation, while a Naive Bayes model based on lipid-class profiles achieved an AUC of 0.79. The results indicate that aromatase inhibitor therapy is associated with systematic lipidomic changes linked to estrogen deprivation and sphingolipid metabolism, providing potential mechanistic insight into long-term metabolic effects of treatment.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926">Aromatase Inhibitor Therapy Is Associated with Distinct Plasma Lipidomic Profiles in Postmenopausal Breast Cancer Patients&nbsp;</a></h4><div>Aleksandra Arsic, Ales Kvasnicka<a target="_blank" rel="noopener noreferrer" href="https://orcid.org/0000-0001-5973-316X"><img></a>, David Friedecky<a target="_blank" rel="noopener noreferrer" href="https://orcid.org/0000-0002-3448-9073"><img></a>, Nebojsa Ivanovic, Maja Milosevic<a target="_blank" rel="noopener noreferrer" href="https://orcid.org/0000-0002-0985-0728"><img></a> and Vesna Vucic</div>Int. J. Mol. Sci. 2026, 27(4), 1926<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.3390/ijms27041926">https://doi.org/10.3390/ijms27041926</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.<div><div>Breast cancer (BRC) is the most common malignancy among women, with over 2.2 million new cases diagnosed globally in 2022 [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B1-ijms-27-01926">1</a>]. A substantial proportion of breast cancers are estrogen receptor-positive (ER+) and/or progesterone receptor-positive (PR+) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B2-ijms-27-01926">2</a>], necessitating multimodal treatment approaches, including surgery, chemotherapy, radiotherapy, and endocrine therapy. Aromatase inhibitors (AIs) represent the gold-standard adjuvant endocrine treatment for postmenopausal women, as they improve disease control and reduce the risk of distant metastasis by inhibiting peripheral aromatase activity [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B3-ijms-27-01926">3</a>]. This inhibition prevents the conversion of androgenic precursors into estrogens in peripheral tissues, resulting in a marked reduction in systemic estrogen levels. For postmenopausal women with ER+ and PR+ breast tumors, a five-year course of AI therapy, often extended beyond this duration, is recommended to reduce the risk of disease recurrence [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B4-ijms-27-01926">4</a>].AIs decrease plasma and tissue concentrations of endogenous estrogens by inhibiting aromatase (cytochrome P450), the rate-limiting enzyme responsible for estrogen biosynthesis from testosterone and adrenal androgens [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B5-ijms-27-01926">5</a>]. Because estrogen plays a protective role in lipid metabolism, AI therapy is frequently associated with dyslipidemia. Increased levels of triglycerides (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL) have been reported in patients receiving AIs [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B6-ijms-27-01926">6</a>], thereby increasing the risk of cardiovascular disease (CVD) in postmenopausal women with BRC [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B7-ijms-27-01926">7</a>].Disturbances in lipid metabolism are recognized as a hallmark of cancer, leading to increasing interest in lipidomic profiling as a tool for characterizing tumor-associated metabolic alterations [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B8-ijms-27-01926">8</a>]. In recent years, clinical lipidomics studies have been conducted across multiple malignancies, including lung [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B9-ijms-27-01926">9</a>], pancreatic [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B10-ijms-27-01926">10</a>], renal cell carcinoma [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B11-ijms-27-01926">11</a>], colorectal [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B12-ijms-27-01926">12</a>], and BRC [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B13-ijms-27-01926">13</a>], highlighting cancer-specific lipidomic signatures and the growing relevance of lipid metabolism in oncology. Lipidomic reprogramming is now recognized as a defining feature of BRC. To date, elevated levels of phosphatidylcholine (PC 32:1), stearic acid, and ceramide (Cer 43:1), along with reduced levels of diacylglycerol (DG 34:2), have been reported in BRC patients compared with healthy women [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B14-ijms-27-01926">14</a>]. These lipid species, together with phosphatidylinositol (PI 16:0/16:1) and PI (18:0/20:4), have been proposed as potential biomarkers for BRC [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B14-ijms-27-01926">14</a>].Despite these advances, there remains a paucity of studies investigating the lipidomic profiles in BRC patients in relation to endocrine therapy. The present cross-sectional study aims to address this gap by conducting mass spectrometry-based profiling of lipid classes and comparing profiles between postmenopausal BRC patients receiving AI therapy and another cohort assessed before this treatment. This approach aims to characterize associations between AI therapy status and plasma lipidomic patterns.<h4>2. Results</h4><h5>2.3. Lipidomic Analysis</h5>Plasma lipid profiling identified 649 lipids across 23 lipid classes and subclasses, covering the major structural families of the circulating lipidome, including neutral lipids (triacylglycerols, diacylglycerols, cholesteryl esters, and cholesterol), glycerophospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylglycerols, phosphatidylserines, and their lysophospholipid counterparts), sphingolipids (sphingomyelins, ceramides, monohexosylceramides, and dihexosylceramides), and free fatty acids. In addition to diacyl phospholipids, the dataset included ether-linked and plasmalogen species (denoted as O- and P-), most prominently within PC, PE, and LPC and LPE classes. An overview of the plasma lipidome is shown as a global lipid network (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#fig_body_display_ijms-27-01926-f001">Figure 1</a>). To assess whether the observed lipid changes reflect coordinated remodeling rather than isolated signals, we performed Fisher’s combined probability test at the lipid-class level. This analysis revealed significant cumulative differences in several lipid classes, including Cer, LPC, ether/plasmalogen LPC, LPE, and SM (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#fig_body_display_ijms-27-01926-f001">Figure 1</a>), supporting systematic class-level alterations despite limited power at the single-species level.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Int_J_Mol_Sci_2026_27_4_1926_Figure_1_Global_lipid_network_analysis_of_plasma_lipidomic_differences_between_breast_cancer_patients_BRC_prior_to_initiation_of_the_aromatase_inhibitor_6f82827773.jpg" alt="Int. J. Mol. Sci. 2026, 27(4), 1926: Figure 1. Global lipid network analysis of plasma lipidomic differences between breast cancer patients (BRC) prior to initiation of the aromatase inhibitor (AI) therapy and patients receiving AI. Cytoscape-based network visualization illustrates lipid species grouped according to structural similarity and lipid class. Each node represents an individual lipid species, while edges indicate class-based relationships. Node size corresponds to the −log10 p-value from the Mann–Whitney U test, reflecting the statistical significance (−log p-Value &gt; 1.3) of differences between groups. Node color indicates the direction and magnitude of change, based on log2 fold change, with red representing higher abundance in AI-treated patients and blue representing higher abundance in patients before AI therapy. Major lipid classes, including glycerophospholipids (GPL), sphingolipids (SP), glycerolipids (GL), fatty acids (FA), and cholesteryl esters (CE), are shown to highlight coordinated class- and species-level lipidomic alterations. Fisher’s combined p-value for individual lipid classes is shown at the bottom (significant p-Value &lt; 0.05). " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Int_J_Mol_Sci_2026_27_4_1926_Figure_1_Global_lipid_network_analysis_of_plasma_lipidomic_differences_between_breast_cancer_patients_BRC_prior_to_initiation_of_the_aromatase_inhibitor_6f82827773.jpg 245w," sizes="100vw" width="1600" height="847"><h5>2.4. Multivariate Analysis of Plasma Lipidomic Profiles</h5>Principal component analysis (PCA) and partial least squares discriminant analysis (OPLS-DA) were applied to the lipidomic dataset to assess global variation and group separation between the BRC and AI groups (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#fig_body_display_ijms-27-01926-f002">Figure 2</a>). PCA was performed as an unsupervised analysis, including all samples and quality control (QC) samples. The first two principal components explained 37.9% (PC1) and 15.6% (PC2) of the total variance, accounting for 53.5% of the cumulative variance. QC samples clustered tightly near the center of the PCA score plot, indicating analytical stability and reproducibility of the LC–MS measurements. In the PCA score plot, samples from the BRC and AI groups showed partial overlap, with no complete separation observed along the first two principal components. To further evaluate group discrimination, a supervised OPLS-DA model was constructed using the same dataset. The OPLS-DA score plot demonstrated improved separation between the BRC and AI groups along the first latent variable, with group-specific clustering highlighted by confidence ellipses. Permutation testing confirmed that the supervised OPLS-DA model was not overfitted, with a negative Q2 intercept (Q2 = −0.55), indicating that permuted models lacked predictive ability compared with the original model (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#app1-ijms-27-01926">Supplementary Figure S1</a>). Together, these multivariate analyses indicate differences in lipidomic profiles between the BRC and AI groups, while also demonstrating acceptable analytical performance as evidenced by the clustering of QC samples.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Int_J_Mol_Sci_2026_27_4_1926_Figure_2_Multivariate_analysis_of_plasma_lipidomic_profiles_in_breast_cancer_patients_BRC_56f65f265b.jpg" alt="Int. J. Mol. Sci. 2026, 27(4), 1926: Figure 2. Multivariate analysis of plasma lipidomic profiles in breast cancer patients (BRC) prior to initiation of the aromatase inhibitor (AI) therapy and patients receiving AI. Principal component analysis (PCA) score plot (a) shows the distribution of plasma lipidomic profiles from patients before AI therapy (BRC, blue), patients receiving AI therapy (AI, red), and quality control samples (QC, gray). Orthogonal partial least squares–discriminant analysis (OPLS-DA) score plot (b) demonstrates improved separation between BRC and AI groups. The ellipse represents the 95% Hotelling’s T2 confidence interval. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Int_J_Mol_Sci_2026_27_4_1926_Figure_2_Multivariate_analysis_of_plasma_lipidomic_profiles_in_breast_cancer_patients_BRC_56f65f265b.jpg 245w," sizes="100vw" width="1600" height="652"><h4>4. Materials and Methods</h4><h5>4.5. Lipidomic Analysis</h5>Chromatographic separation was performed using an ultra-high-performance liquid chromatography (UHPLC) <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=55&amp;search=ExionLC">ExionLC system</a> (SCIEX, Framingham, MA, USA) coupled to a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=55&amp;search=6500">QTRAP® 6500+ mass spectrometer </a>(SCIEX, Framingham, MA, USA). Lipids were separated on an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1953">ACQUITY Premier BEH C8 column</a> (100 × 2.1 mm, 1.7 μm; Waters Corporation, Wilmslow, UK). The column temperature was kept at 55 °C, and the autosampler was set to 10 °C. The mobile phases consisted of solvent A (acetonitrile/water, 6:4, v/v, containing 10 mM ammonium acetate) and solvent B (isopropanol/acetonitrile, 9:1, v/v, containing 10 mM ammonium acetate), delivered at a flow rate of 0.4 mL/min. The total analytical run time was 20 min per sample. Data acquisition was carried out using Analyst software (version 1.6.2), and data processing and semiautomatic peak integration were performed with MultiQuant software (version 3.0; SCIEX, Framingham, MA, USA). Samples were analyzed in a randomized sequence with continuous monitoring of quality control samples. Raw peak areas were QC-normalized by LOESS [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B48-ijms-27-01926">48</a>] and IS-normalized by the corresponding IS class for each lipid.<h5>4.6. Statistical Analysis</h5>The data were processed using the Metabol package [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B48-ijms-27-01926">48</a>] in R program (v 3.6.3). Preprocessing of the data included locally estimated scatterplot smoothing (LOESS), Pareto scaling, filtering of analytes (coefficients of variation in the QC above 30%), mean centering, and the lnPQN normalization (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#app1-ijms-27-01926">Supplementary Table S1</a>) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B49-ijms-27-01926">49</a>]. Univariate statistical analysis (based on the non-parametric non-paired Mann–Whitney U test (p-values shown as −log) together with the log2 fold-change in medians) was performed and visualized using GraphPad Prism (version 9.0, GraphPad Software, LLC, Boston, MA, USA). Data were also evaluated using multivariate statistical analysis, namely the principal component analysis (PCA) and orthogonal discriminant analysis by partial least squares (OPLS-DA) and validated by permutation using SIMCA software (version 18.0, Sartorius Umetrics, Umeå, Sweden). Benjamini–Hochberg correction was used for false discovery rate correction. Detected lipid species were mapped to standardized lipid classes and subclasses according to accepted lipidomics nomenclature, enabling consistent grouping of lipid species for downstream statistical, class-level, and network analyses. LipidOne 2.4 was further used to support lipid-centric data integration and visualization prior to multivariate and pathway-based analyses (such as heatmaps, ROC analyses using a Naive-Bayesian model and Logistic Regression model, and protein–protein interaction network based on lipidomics data) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B50-ijms-27-01926">50</a>]. Lipid network visualization was performed using Cytoscape software (v 3.8.2) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B51-ijms-27-01926">51</a>]. Differentially abundant lipid species were organized according to lipid class and structural similarity. Node size reflected the −log10 p-value from univariate analysis, while node color represented log2 fold change between groups. Calculation of Fisher’s combined probability test for assessing systematic lipid class-level differences was performed (<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#app1-ijms-27-01926">Supplementary Table S2</a>) and calculated following procedures described previously [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/1422-0067/27/4/1926#B52-ijms-27-01926">52</a>].<h4>5. Conclusions</h4>In conclusion, our findings demonstrate significant alterations in lipid metabolism in BRC patients undergoing AI therapy, with pronounced changes in Cer, SM, DG, TG, PC, PI, and PE lipids and lipid classes. However, the precise mechanisms underlying the differences in lipid metabolism between patients before and during AI treatment remain to be fully elucidated. Future longitudinal studies with paired pre- and post-treatment samples from the same individuals leveraging comprehensive lipidomic profiling are needed to deepen our understanding of AI-induced metabolic alterations and to inform the development of targeted interventions aimed at mitigating adverse effects, ultimately improving long-term outcomes and quality of life for these patients.</div></div>

Aromatase Inhibitor Therapy Is Associated with Distinct Plasma Lipidomic Profiles in Postmenopausal Breast Cancer Patients

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system

The new Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system delivers exactly what a busy analytical testing lab is looking for: consistently excellent results-every shift, every day, for every operator.

<h3>Welcome to the evolution of everyday IC reliability</h3>The new Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system makes ion analysis simpler and more intuitive than ever before. And it delivers exactly what a busy analytical testing lab is looking for: consistently excellent results-every shift, every day, for every operator.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_2_6400708696.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_2_6400708696.jpg 245w," sizes="100vw" width="825" height="498"><h3>SIMPLE, INTUITIVE USER EXPERIENCE&nbsp;</h3><ul><li>Smart, function-driven design lets you quickly and safely access everything on the instrument—no hard-to-reach compartments, and no step stools or extra tools needed!</li><li>Automated startup and shutdown routines ensure the system is quickly ready for the day’s work without user intervention.&nbsp;</li><li>Built-in how-to videos reduce training time and simplify setup and operation.</li></ul><h3>ULTRA-RELIABLE PERFORMANCE&nbsp;</h3><ul><li>Advanced high-performance pump technology and electronics let you take advantage of a wider range of columns and chemistries to improve the speed and quality of your results.</li><li>Fast, flexible, convenient electrolytic suppression: Just connect the suppressor; no external pump or offline conditioning needed for regeneration.</li><li>Reagent-free IC (RFIC™) capability makes eluent preparation as simple as “just add water,” while ensuring more consistent results and enabling optimized gradient separations.</li></ul><h3>EASILY CONFIGURABLE AND UPGRADEABLE</h3><ul><li>Versatile, adaptable platform lets you configure the exact Dionex Inuvion IC system for today’s needs and budget, with confidence that your system will also meet your future needs.</li><li>Multiple options and peripherals extend IC capabilities, letting you easily—and cost-effectively—adapt to changing sample types and workflow requirements.</li></ul><h3>Simply the best system for everyday ion analysis</h3><figure class="image"><img style="aspect-ratio:1107/755;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_d855feb2a8.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_d855feb2a8.jpg 229w," sizes="100vw" width="1107" height="755"></figure>1. Operator-friendly engineering saves time and reduces errors by allowing easy, unobstructed access to all components, connections and consumables2. Compact design helps you make the most of valuable bench space3. Digital gas pressure regulator increases precision of gas delivery for maximum day-to-day consistency (optional)4. Temperature-controlled conductivity cell improves reproducibility and ensures baseline stability5. Vacuum degassing capability enables more consistent peak identification from smoother baselines6. Seal wash pump reduces maintenance costs when using manually prepared eluents (optional)&nbsp;7. Reagent-free IC (RFIC) saves time and ensures greater day-to-day and operator-to-operator consistency, while also allowing the use of gradient separations8. Electrolytic suppression entirely eliminates the need for manual regenerant preparation (or, opt for a continuously regenerated, always- ready-to-go chemical suppressor)9. Thermo Scientific™ Dionex™ IC PEEK Viper™ fittings ensure confident, low dead volume connections every time—no tools required! (optional)10. Integrated column heater minimizes the impact of fluctuations in lab temperature that can adversely affect results (optional)11. 6- or 10-port auxiliary valve lets you automate in-line sample preparation12. State-of-the-art high-pressure pump (up to 5000 psi) lets you increase flow rates with smaller particle columns to reduce run times or add additional separation capacity with longer column length13. System self-diagnostics automatically detect any issues with hardware and consumables14. Self-service capabilities and user-swappable parts help you get back up and running in a hurry Automatic eluent monitor alerts the operator to low solution levels, avoiding potential system damage or failed runs (optional)15. Automatic eluent monitor alerts the operator to low solution levels, avoiding potential system damage or failed runs (optional)<h3>Flexible, adaptable Dionex Inuvion platform</h3>You can configure your Dionex Inuvion system to meet specific analytical and budget requirements, and easily reconfigure as needed. Multiple configurations and peripherals extend IC capabilities, letting you easily-and cost-effectively-adapt to changing sample types and workflow requirements.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_3_fa8233bcd4.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_Inuvion_ion_chromatography_system_3_fa8233bcd4.jpg 245w," sizes="100vw" width="1246" height="572"><h3>Consistently excellent results every day</h3>The Dionex Inuvion IC system supports a full range of columns to improve the speed and quality of results, for both anions and cations. Expanded capabilities, such as Reagent-Free IC (RFIC), enhance operator-to-operator consistency, while enabling the use of eluent gradients to optimize separations.<h4>Rapid results</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_2_Ukazka_rychle_separace_zakladnich_aniontu_s_uhlicitanovou_MF_Zkraceni_doby_analyzy_diky_typu_kolony_a_vyssimu_prutoku_0e610b919d.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system: Figure 1. Elution of seven common inorganic anions using carbonate eluent. Short columns and fast flow rates let you reduce run times." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Obr_2_Ukazka_rychle_separace_zakladnich_aniontu_s_uhlicitanovou_MF_Zkraceni_doby_analyzy_diky_typu_kolony_a_vyssimu_prutoku_0e610b919d.jpg 245w," sizes="100vw" width="1622" height="739"><h4>Exceptional reproducibility</h4><figure class="image"><img style="aspect-ratio:1693/754;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_3_Ukazka_reprodukovatelnosti_analyzy_zakladnich_aniontu_40_nastriku_Spickova_technologie_cerpadla_a_elektronika_pristroje_poskytuji_konzistentni_a_reprodukovatelne_vysledky_f66e7430cf.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system: Figure 2. Overlay of 40 replicate injections. State-of-the-art pump technology and Electronics deliver consistent, reproducible results." width="1693" height="754"></figure><h4>Increased sensitivity and consistency</h4><figure class="image"><img style="aspect-ratio:1860/836;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_4_Ukazka_vysoce_citlive_analyzy_aniontu_ve_vodach_Eluent_generator_KOH_a_elektrolyticka_suprese_snizuji_pozadi_cimz_dochazi_ke_zvyseni_citlivosti_da55facfd0.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system: Figure 3. Determination of inorganic anions in drinking and surface waters. KOH eluent generation and electrolytic suppression yield lower backgrounds for greater sensitivity and increased day-to-day consistency." width="1860" height="836"></figure><h4>Method optimization</h4><figure class="image"><img style="aspect-ratio:1799/889;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Obr_5_Ukazka_optimalizace_metody_zkraceni_doby_analyzy_pouzitim_gradientu_mobilni_faze_fc1fbe9cb0.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system: Figure 4. Comparison of isocratic and gradient runs using eluent generation. Reduce run length and sharpen later-eluting peaks with the same ease as an isocratic run with no need for two separate eluents or a proportioning pump." width="1799" height="889"></figure><h3>Compliance-ready Chromeleon CDS</h3>A trusted industry standard all over the world, ”Simply Intelligent” <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) software</a> brings out the full power of the Dionex Inuvion IC system. Easy to learn and easy to use, it automates and optimizes daily tasks and helps even inexperienced users achieve consistent, high quality results.<figure class="image"><img style="aspect-ratio:3700/1660;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Thermo_Scientific_Dionex_Inuvion_2_303a6eb73b.jpg" alt="Thermo Scientific™ Dionex™ Inuvion™ ion chromatography system." width="3700" height="1660"></figure>

Watrex Streamline GPC system

GPC system is an isocratic HPLC system consisting of one P1 pump, CT1i column thermostat with manual injector and RI1 refractive index detector.

GPC system is an isocratic HPLC system consisting of one P1 pump, CT1i column thermostat with manual injector and RI1 refractive index detector.<h3>FEATURES</h3><ul><li>Optimized pump mechanics of P1 pump with parallel piston design offers excellent flow rate accuracy and low RI signal pulsation.</li><li>The thermal isolated optic with a counter-current heat exchanger and programmable temperature control, results in an extremly stable baseline and an optimal Signal/Noise ratio od RI1.</li><li>The system can be upgraded with autosampler and fraction collector, or VD 400 Viscometer Detector.</li><li>Data acquisition, processing and instrument control is done by Clarity Chromatography Station with GPC option or Agilent TriSEC software.</li></ul><h3>TECHNICAL SPECIFICATION</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=streamline+p1+pump&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">P1 HPLC pump: Flow Rates</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/detectors/other-hplc-detectors/streamline-ri1-detector/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">RI1 refractive index detector</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/column-thermostats/streamline-ct1-columm-thermostat/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">CT1 Column thermostat</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity™ Chromatography Station</a></li></ul><h5>COMPONENT SPECIFICATION - P1 HPLC pump: Flow Rates</h5><ul><li>Flow Rates: 0.001 to 10.000 mL/min</li><li>Pressure: 0 to 6,000 psi (42 MPa)</li><li>Flow precision: &gt; 0.2% RSD</li><li>Flow accuracy: ± 1 % @ 1mL/min and 7 MPa,</li><li>Pressure accuracy: ± 2% of full-scale, with 80:20 H2O/IPA @ 7 MPa</li><li>Pulsation: ± 0.5 % @ 1mL/min and 7 MPa</li><li>Remote inputs: USB</li></ul><h5>COMPONENT SPECIFICATION - RI1 refractive index detector</h5><ul><li>Light Source: Long-life LED</li><li>Refractive index Range: 1.00 - 1.75 RIU</li><li>Baseline Noise: ± 2.5 nRIU</li><li>Baseline Drift: 200 nRIU/h</li><li>Linearity: &gt; 1000 μRIU</li><li>Flow cell pressure resistance: 5 bar</li><li>Flow cell volume: 15 μl</li><li>Time constant: 0.00/0.01/0.02/0.05/0.1/0.2/0.5/1.0/2.0/5.0/10.0 s</li><li>Analog Output: 1x 0-2.5V</li><li>Outputs: Event 1, Start (OUT), Error (OUT), + 5 V, 24 V Valve</li></ul><h5>COMPONENT SPECIFICATIONS - CT1 Column thermostat</h5><ul><li>Temperature range: 10 °C above ambient temperature to 100 °C</li><li>Temperature accuracy: max. 0,5 % of actual temperature</li><li>Temperature stability: RSD &lt; 0,4 %</li><li>Settability: 0,1 °C</li><li>Internal dimensions: 320 x 50 x 30 mm (L x W x H)</li><li>Compartment material: PTFE coated metal</li><li>Compartment connection: USB</li><li>Control: build-in color touch panel, datasystem</li></ul><h5>COMPONENT SPECIFICATIONS - <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity™ Chromatography Station:</a></h5>Advanced chromatographic datastation for data acquisition and evaluation supported with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1004">GPC module for MWD, Mw, Mn, Mz calculation</a>. Enables rutine separations with pre-set methods, containing all experimental and conditions and data evaluation settings. PC Win 10 Professional with LCD monitor included.<h3>Ordering information:</h3><h5>PN/Component:</h5><ul><li>SGPC01-001 Streamline™ manual GPC system with Refractive index detector (Includes P1 pump, RI1 detector, CT1i column thermostat with manual injector, solvent rack, Clarity PC station)</li></ul>Optional configurations:<ul><li>ST99-002 Remove CT1i column thermostat with manual injector from system</li><li>SAS90-001 Add AS1 Autosampler to system</li><li>SFC90-001 Add FC1 Fraction collector to system</li></ul>

SIELC Alltesta Gradient Automated HPLC Analyzer

SIELC’s Alltesta™ Analyzer is an affordable HPLC analytical system with for measuring small and trace concentrations of various chemicals.

SIELC’s Alltesta™ Analyzer is an affordable HPLC analytical system that provides both scientific and educational environments with a convenient, simple solution for measuring small and trace concentrations of various chemicals in products such as milk, baby formula, pet food, pills, household formulations, and more.No difficult sample preparation is necessary, and no prior experience in chromatography or analytical chemistry is required for the operator. Measurements typically take less than 5 minutes, and the Alltesta™ comes with a set of supplies sufficient for 200 analyses.<ul><li>Small foot-print, light-weight, can be used on a small table</li><li>No difficult sample preparation</li><li>Takes only a few minutes to start operating</li><li>No chemical experience is required for instrument user</li><li>User friendly, self explanatory cloud-based software</li><li>Produces easy-to-understand results within 5-10 minutes</li></ul><h4>Alltesta Gradient Automated HPLC Analyzer includes:</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1249">Alltesta™ Power Tower</a> (provides power and cloud connectivity)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1244">Alltesta Mini Autosampler</a> (includes <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1245">Alltesta High-Pressure Switching Valve</a>)</li><li>Two <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1247">Alltesta™ Ultra High Pressure Mini Syringe Pumps</a> for gradient flow</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1248">Alltesta UV-Vis Detector</a></li></ul><h4>Examples of Alltesta™-based HPLC methods</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Aspirin_in_Alka_Seltzer_Extra_Strength_Effervescent_Aspirin_on_Primesep_100_Column_d33816ae83.pdf">HPLC Method for Analysis of Aspirin in Alka-Seltzer Extra Strength Effervescent Aspirin</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Phenylephrine_Hydrochloride_1_in_Equate_Nasal_Spray_on_Primesep_100_Column_02f2983c25.pdf">HPLC Method for Analysis of Phenylephrine Hydrochloride 1% in Equate – Nasal Spray</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Rockstar_Zero_Carbs_Energy_Drink_with_Caffeine_and_Taurine_on_Primesep_100_Column_c87a85a0b4.pdf">HPLC Method for Analysis of Rockstar Zero Carbs Energy Drink with Caffeine and Taurine</a></li></ul>See this cloud-based example of the 2-Naphthalenesulfonic acid, Allura red, Fast Green FCF, 1,5-Naphthalenedisulfonic acid, Tartrazine separation method <a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/11972/injection/21307/?t=a992fefcdd3647b19f6bed81e182d3d8">here</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_example_of_the_2_Naphthalenesulfonic_acid_Allura_red_Fast_Green_FCF_1_5_Naphthalenedisulfonic_acid_Tartrazine_separation_b96e648d63.jpg" alt="SIELC: SIELC Alltesta™ Gradient Automated Analyzer - example of the 2-Naphthalenesulfonic acid, Allura red, Fast Green FCF, 1,5-Naphthalenedisulfonic acid, Tartrazine separation.">

SIELC Alltesta Cromite HPLC Analyzer

Compact and cloud-connected, the Cromite™ HPLC System delivers precise UV/Vis analysis with innovative single-ended column technology—ideal for research, QC, and education labs.

The Cromite™ HPLC System from SIELC Technologies is a compact, high-performance liquid chromatography platform engineered for research, teaching, and routine quality control. It combines professional analytical performance with intuitive operation in a lightweight, space-saving design.Designed for academic institutions, research laboratories, and QC environments in pharmaceuticals, food science, and environmental analysis, Cromite™ delivers reliable separations without the complexity of conventional full-scale systems. Its automated single-vial injection, isocratic syringe pump, and UV/Vis detection enable accurate analysis of compounds such as caffeine, vitamins, food dyes, environmental contaminants, and active pharmaceutical ingredients.Cloud-enabled control software allows users to create methods, run analyses, review chromatograms, and share data remotely from virtually any device. This connectivity makes Cromite™ well suited for distributed teams, teaching environments, and flexible laboratory setups.A key innovation is SIELC’s proprietary Single-Ended HPLC Column technology (up to 150 mm length). This design eliminates tubing and fittings, minimizes dead volume, and enables tool-free column replacement, reducing setup time and the risk of leaks. Each system is supplied with a single-ended column of choice and all essential accessories for immediate operation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_Alltesta_Cromite_HPLC_analyzator_285f593640.jpg" alt="SIELC: Alltesta Cromite HPLC analyzer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_Alltesta_Cromite_HPLC_analyzator_285f593640.jpg 234w," sizes="100vw" width="1536" height="1024"><h4>Key Features</h4><ul><li>Compact and Lightweight Design: Weighing only 1.45 kg and measuring 115 × 190 × 115 mm, Cromite™ fits easily into space-limited labs and classrooms.</li><li>Automated Injection: Single-vial automated injection simplifies operation and improves reproducibility.</li><li>Isocratic Syringe Pump: Flow rates from 0.1 to 4.0 mL/min with pressures up to 4000 psi ensure effective separation of complex mixtures.</li><li>UV/Vis Detection<ul><li>Standard wavelengths: 275, 460, 520, 630 nm</li><li>Custom configuration: any four wavelengths starting from 235 nm</li><li>Low noise (&lt;0.05 mAU) and 20 Hz detection rate support reliable quantification.</li></ul></li><li>Cloud-Based Software: Wi-Fi, Ethernet, and mobile connectivity enable remote method development, data analysis, and collaboration.</li><li>Single-Ended Column Technology<ul><li>Zero dead volume design</li><li>No tubing or fittings</li><li>Tool-free replacement</li><li>Supports 4.6, 3.2, and 2.1 mm ID columns up to 150 mm length</li></ul></li></ul><blockquote>We offer a special 15% discount for schools.</blockquote><h4>Examples of Alltesta™-based methods</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12579/injection/22619?t=3f67e3c308cc479085c836cc682e8f1a">HPLC Method of Analysis of Ascorbic Acid</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12533/injection/22589/?t=43cad31c0e924440a18874658565c3db">HPLC Method of Analysis of Theobromine, Theophylline, Caffeine and C7</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12411/injection/22415/?t=0c93f4eb855c43aebcfd4c3b1fd55ce9">HPLC Method of Analysys of Aspirine</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Phenylephrine_Hydrochloride_1_in_Equate_Nasal_Spray_on_Primesep_100_Column_02f2983c25.pdf">HPLC Method for Analysis of Phenylephrine Hydrochloride 1% in Equate – Nasal Spray</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Rockstar_Zero_Carbs_Energy_Drink_with_Caffeine_and_Taurine_on_Primesep_100_Column_c87a85a0b4.pdf">HPLC Method for Analysis of Rockstar Zero Carbs Energy Drink with Caffeine and Taurine</a></li></ul><h4>Specifications</h4><ul><li>Injection: Automatic, 1 vial</li><li>Pump: Syringe, isocratic</li><li>Flow Rate: 0.1–4.0 mL/min</li><li>Maximum Pressure: 4000 psi</li><li>Noise Level: &lt;0.05 mAU</li><li>Detection Rate: 20 Hz</li><li>Wavelengths (standard): 275, 460, 520, 630 nm</li><li>Wavelengths (custom): Any four starting from 235 nm</li><li>Supported Columns: Single-Ended HPLC Columns (4.6, 3.2, 2.1 mm ID)</li><li>Column Length: ≤150 mm</li><li>Connectivity: Wi-Fi, Ethernet, Mobile network</li><li>Power Supply: 100–240 V, 50–60 Hz</li><li>Operating Temperature: 0–40 °C</li><li>Dimensions (W × H × L): 115 × 190 × 115 mm</li><li>Weight: 1.45 kg</li></ul><h4>Typical Applications</h4><ul><li>Pharmaceutical Analysis – API identification, formulation testing, and stability studies</li><li>Environmental Testing – Detection of contaminants in water and soil</li><li>Food &amp; Beverage – Analysis of vitamins, additives, and preservatives</li><li>Academic Laboratories – Hands-on chromatographic training and research</li><li>Materials &amp; Chemical Research – Evaluation of additives, catalysts, and specialty chemicals</li></ul><blockquote>For pricing information, please contact: <a>alltesta@sielc.com</a></blockquote>

ECOM PREPBOX A3L8E Chromatography Separation System

PREPBOX is a compact alternative to modular preparative systems. It is assembled according to the customer's wishes.

<div style="display:block;" aria-labelledby="tab_item-0">Complete preparative modular system with one basic gradient pump and optionally secondary pump for repetitive sampling or with injection valve. Also switching valve for&nbsp;<abbr title="Countercurrent Chromatography">CCC</abbr> applications. PDA four channel detector up to 800&nbsp;nm.&nbsp;It is assembled according to the customer's wishes. It is used for high-capacity systems or with centrifugal chromatography systems like in pharmaceutical purification of cannabis extracts (<abbr title="Cannabidiol">CBD</abbr>, <abbr title="Cannabinol">CBN</abbr>, <abbr title="Cannabigerol">CBG</abbr>, <abbr title="Cannabichromene">CBC</abbr>, <abbr title="Tetrahydrocannabinol">THC</abbr>...).ECOMAC SW installed at internal PC controls the system. Fractions are collected by means of&nbsp;10 position valve&nbsp; It is necessary to purchase an operating system for the PREPBOX&nbsp;- in most cases we recommend Windows OS&nbsp;(P/N:&nbsp;ABB90000) or Linux&nbsp;(on request). The system also supports the Clarity software. It is also possible to purchase a wireless mouse and keyboard set&nbsp;(P/N:&nbsp;EPC-0028) and a computer monitor&nbsp;(P/N:&nbsp;EPC-0027).<h5>TOY18DAD 800L Detector</h5>It is UV-VIS diode array detector, which allow measuring absorbance of four wavelengths in range of 200-800&nbsp;nm simultaneously in one cell just as scanning of whole spectra.<h5>ECP201L Preparative HPLC Pump</h5>Preparative pump suited for preparative liquid chromatography with very high pressure up to 15&nbsp;MPa&nbsp;at flow rate of 1000&nbsp;ml/min. Advantage of this pump is a very stable run even at low flow rates.<h5>ECP2300 Preparative Gradient Pump</h5>Preparative HPLC pump suited for&nbsp;preparative&nbsp;applications in liquid chromatography with a pressure up to 15 MPa at flow rate of 300&nbsp;ml/min.&nbsp;New sophisticated unit allows achieving a precise&nbsp;low-pressure gradient, i.e. gradual changes of solvent composition by mixing&nbsp;up to four liquids&nbsp;at the pump entry.<h3>Parameters</h3><ul><li>Interface: 5x USB, HDMI, 2X RS232, LAN, VGA</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 490 W</li><li>Dimensions W x H x D: 405x490x525 mm (15.94x19.29x20.67 in)</li><li>Weight: 55 kg</li></ul></div>

ECOM COMPACT SEMI-PREPARATIVE SYSTEM (10 ml/min, ECS08)

A highly effective Compact semi-preparative system containing UV-VIS DAD detector, analytical gradient pump with degasser, automatic inject valve and fraction collector.

A highly effective Compact semi-preparative system containing&nbsp;UV-VIS DAD detector, analytical gradient pump with degasser, automatic inject valve and fraction collector. The detector allows absorbance measuring at four wavelengths at the same time or scanning through entire spectrum.The system is controlled by a built-in PC and touchscreen.A modular solution allows configuring the unit according to customer needs. This system contains a detector with a wavelength range of 200 –&nbsp; 800 nm, a pump with a flow rate up to 10 ml/min and a fraction collector.<h3>Main parts</h3><h5>TOY18DAD800L DETECTOR</h5>It is a UV-VIS diode array detector, which allows measuring the absorbance of&nbsp;four wavelengths in a range of 200–800 nm simultaneously in one cell&nbsp;just as scanning of whole spectra. Standardly is assembled with preparative cell PLCC07L, but other cells are available.<h5>ECF2096 FRACTION COLLECTOR</h5>Fractions are collected according to chosen method, which is simply created using a touch screen. Racks for 8, 21,40 ml tubes, 60 ml vials or 12 funnels, 30 mm.<h5>AUTOMATIC INJECT VALVE</h5>The valve is driven by a step motor and it is automatically switched by software. The system also allows manual sample injection by opening the column.<h5>SYSTEM PC INFO</h5>Installed industrial PC with Intel Atom 4 core processor. OS Linux, Touch screen 12,1“ 1024 x 786, SW QUEEN controls: gradient, flow rate, wavelengths, fractions collection etc. Remote administration is available. Possibility of measuring at four wavelengths at the same time or scanning through entire spectra. Furthermore, the possibility of displaying 3D data.&nbsp;<h5>ECP2010 ANALYTICAL GRADIENT PUMP</h5>Modified for the compact system,&nbsp;analytical gradient pump with degasser&nbsp;and with flow rate up to 10 ml/min&nbsp;(40 MPa, 5082 PSI).&nbsp;Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions. Four gradient valves allow changing mobile phases without bottles alternation.&nbsp;<h3>COMPACT PREPARATIVE SYSTEM &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</h3><ul><li>Interface: 3xUSB, 2xLAN, RS232</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 400 W</li><li>Dimensions (W x H x D): 500 x 678 x 482 mm (19.69 x 26.69 x 18.98")</li><li>Weight: 55 kg (121.30 lb)</li></ul><h5>Detector</h5><ul><li>Wavelength range: 200 - 800 nm &nbsp; (256 elements on CCD)</li><li>Number of channels (Signals): 4</li><li>Scan: 200 - 800 nm,up to &nbsp;20 Hz, step 1 nm</li><li>Typical spectral half-width: 10 mn</li><li>Accuracy of adjustment / Reproducibility: ± 1 nm / ±0.5 nm</li><li>Noise level at test cell (254 nm, TC 0.75s): ± 5 x 10-5 AU</li><li>Drift at test cell (254 nm after 1 h): 1 x 10-3 AU/hr</li><li>Materials in contact with mobile phase: FEP; fused silica, stainless steel, PEEK</li><li>Time constant (T63): 0.5 s, 0.75 s, 1.0 s, 2.0 s, 4.0 s, 8.0 s, 16.0 s, 0.2 s, 0.1 s</li></ul><h5>Fraction collector</h5><ul><li>Available racks (two racks per unit)<ul><li>EC08 48 tubes of 8 ml</li><li>EC21 36 tubes of 21 ml</li><li>EC40 24 tubes of 40 ml</li><li>EC60 20 vials of 60 ml</li><li>F12A 12 funnels, 30 mm</li></ul></li><li>Maximum flow rate for tubes 8 ml: max. 50 ml/min</li><li>Maximum flow rate for tubes 21, 40 ml: max. 300 ml/min</li><li>Maximum flow rate for vials 60 ml: max. 300 ml/min</li><li>Maximum flow rate for funnels: max. 500 ml/min</li><li>Wetted materials: FEP, Tefzel®, PPS, KEL-F, PTFE, PEEK, SS 316, SIMAX glass</li><li>Input tubing: OD 1/8“ x ID 1/16“ FEP</li></ul><h5>Pump</h5><ul><li>Flow rate: 0.02 - 10.00 ml/min</li><li>Maximum operating pressure: 40 MPa (5082 PSI)</li><li>Repeatability of flow rate adjusting: ± 0.5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Wetted materials: stainless steel, sapphire, KEL-F, seals*</li><li>Output capillary outer diameter: 1/16”</li><li>Input tubing outer diameter: 1/8”</li><li>Gradient valves control module - OPTIONALLY: Up to four valves</li></ul>

Waters Alliance iS HPLC System

Waters™ delivers a reliable partner for QC labs. The Alliance™ iS HPLC System enhances efficiency, reduces errors, and simplifies workflows - your new essential lab ally.

<h3>The Alliance iS HPLC System didn’t start in our lab.</h3>It started in yours.Pharmaceutical Quality Control (QC) labs face relentless demand to do more with less amid higher staff turnover, growing levels of sample complexity, and the drive to reduce errors.With regulatory scrutiny and faster time to market fueling these intense demands, the need for simplicity has never been greater.Waters™ understands the challenges you face and is ready to deliver a true ally for your laboratory. With capabilities developed specifically for the QC laboratory, the Alliance™ iS HPLC System will quickly become your favorite ally.<ul style="list-style-type:disc;"><li>Intuitively prevents up to 40% of common errors*</li><li>Provides fast, simple guidance at the point of need</li><li>Boosts productivity and capacity through effective resource utilization and error-free operation</li><li>Drives workflow efficiencies and quality improvements</li><li>Improves data integrity</li></ul>*Based on Waters’ market research in 2022, surveying 56 global Pharma QC labs running &gt;25 systems<h3>Common challenges facing the QC lab</h3>What you are telling us<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_cc30c5f925.jpg" alt="Waters Alliance iS HPLC System - What you are telling us" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_cc30c5f925.jpg 245w," sizes="100vw" width="2038" height="1132"><h3>Meet your new lab ally</h3>Intuitive and reliable, the Alliance iS HPLC System is designed to work seamlessly with Empower™ Software and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720007883en_41b7055d98/720007883en.pdf">eConnect™ Column Tag Technology</a>, unlocking unique features and functionality that eliminate the need to choose between simplicity, efficiency, and quality.<h3>Step-change the performance of your lab</h3>Achieve First Time Quality (FTQ) with the Alliance iS HPLC SystemThe Alliance iS HPLC System supports FTQ by reducing the risk of common errors and improving the reliability of analytical measurements. Your lab can reduce reagents and consumables waste, and automated system checks help to meet critical error reduction KPIs. The increase in operational efficiency and productivity will save time and money and boost the capacity of your lab.**With ease of use at the heart of the user experience, the Alliance iS HPLC System identifies potential errors earlier, reducing the number of retests and failure investigations. This supports a Quality culture resulting not only in safer products critical to human health and well-being, but also an improved working environment, directly impacting employee retention and motivation.****vs the Alliance HPLC System<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_2_44577b6737.jpg" alt="Waters Alliance iS HPLC System with bottles connected." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_2_44577b6737.jpg 234w," sizes="100vw" width="1024" height="682"><h3>The System: intuitively simple</h3><h5>System Status Lighting and Solvent Tubing Clips&nbsp;</h5>Status lighting provides you with a clear visual indication of the system status (in use, idle etc), while the color-coded solvent tubing clips ensure the tubing remains organized to enable tracing from instrument to the solvent bottle flags.<h5>Made for Empower</h5>Waters Empower Software Suite helps your entire laboratory operate better, with the industry’s most advanced data acquisition, management, processing, analytics, and reporting capabilities. Empower Software provides you an environment where your records are traceable, so you always have full control of your most critical asset - your data.<h5>waters_connect™ System Monitoring&nbsp;</h5>Maximize lab productivity - utilize resources more effectively with real-time lab management from anywhere, anytime. System Monitoring provides intuitive and centralized access to detailed Empower Software instrument information via the waters_connect Cloud Platform, without compromising security or compliance.<h5>Intelligent Method Translator App&nbsp;</h5>Working with Empower Software, the iMTA enables you to create an Alliance iS HPLC System Instrument Method directly from a Waters or other third-party LC system. Basic chromatographic conditions are automatically transferred for you, and the new Empower instrument method can be reported, providing traceability to the original method for compliance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_with_bottles_connected_and_the_doors_open_ff4184918a.jpg" alt="Waters Alliance iS HPLC System with bottles connected and the doors open." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_with_bottles_connected_and_the_doors_open_ff4184918a.jpg 234w," sizes="100vw" width="1024" height="682"><h3>Say ‘hi’ to your lab ally</h3><h5>Intuitive touchscreen&nbsp;</h5>An easy-to-use, advanced touchscreen integrates with Empower Software and provides easy-to-follow steps. Pre-run checks for common errors such as missing vials, incorrect column, and qualification/preventative maintenance requirements enable you to catch these mistakes before they result in a sample analysis failure, preventing re-runs.<h5>Help at your fingertips&nbsp;</h5>The Alliance iS HPLC System support journey delivers smart on-board technology that digitally brings you straight to the information you need at the new Waters Help Center. Train and troubleshoot with ease, knowing that Waters will support you at every stage.<h5>Smart column capabilities&nbsp;</h5>Ensure complete column traceability and facilitate post-run troubleshooting with the eConnect HPLC Columns. Ready-to-use from the box, eConnect columns are securely fitted with an NFC-enabled eConnect device tag, ensuring they are always automatically identified, verified, and tracked by the system. These second-generation smart columns propel today’s QC laboratory toward an entirely digital platform and away from manual logbooks.<h3>Enjoy stress-free analysis</h3>The Alliance iS HPLC System keeps you and your lab stress-free and running smoothly with an intuitive touchscreen that supports the productivity and efficiency of your lab.<ul><li>Start your day easily with a system guided start-up.</li><li>Pre-run checks to minimize sample analysis failures due to human error.</li><li>When issues arise, Alliance iS HPLC System provides you with information about what needs attention.</li><li>Guided workflows provide step-by-step guidance on how to resolve common issues.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_2_0e64835283.jpg" alt="Waters Alliance iS HPLC System - Enjoy stress-free analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_2_0e64835283.jpg 204w," sizes="100vw" width="1388" height="1063"><h3>Built to perform, time after time</h3><h4>Intuitive innovation</h4>Developed with the singular goal of providing innovation that solves the problems that matter in the QC lab, the Alliance iS HPLC System includes a range of new and unique features.&nbsp;<ul><li>New electronics to enable the Alliance iS HPLC System smart features (including advanced touchscreen, eConnect Column Tag Technology, and unparalleled interactivity with Empower Software)</li><li>Redesigned check valves and mixer for increased performance and robustness</li><li>Faster detector warm-up time and reduced drift to ensure you’re up and running quickly, for as long as your analysis requires</li><li>Robust performance when operating at the extremes of the Column Heater Cooler and Sample Manager temperature ranges</li><li>Class leading carryover performance and injection precision</li></ul><figure class="image"><img style="aspect-ratio:1561/740;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_3_9aab20fd13.jpg" alt="Waters Alliance iS HPLC System - Overlay of 42 injections of gradient performance mix over 44 hours demonstrated RT repeatability of 0.5% RSD on the Alliance iS HPLC System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_3_9aab20fd13.jpg 245w," sizes="100vw" width="1561" height="740"></figure><h3>Alliance iS HPLC System gives you so much more</h3>Unlock new potential with Empower SoftwareThe Alliance iS HPLC System works exclusively with our <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008057en_af76145fe7/720008057en.pdf">Empower Software</a> version 3 Feature Release 4 or later, with fully enabled and differentiated features, starting with eConnect Technology. Empower Software elevates your quality, your business, and your lab.<ul style="list-style-type:disc;"><li>Intuitive method matching</li><li>Bi-directional touchscreen interactivity</li><li>Column tracking and history</li><li>Enhanced instrument traceability</li><li>Pre-run checks</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_4_430a084194.jpg" alt="Waters Alliance iS HPLC System gives you so much more." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_4_430a084194.jpg 245w," sizes="100vw" width="1385" height="674"><h3>Help at your fingertips</h3>From onscreen to onsite, we’re there for you.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_5_df4f1467ed.jpg" alt="Waters Alliance iS HPLC System - From onscreen to onsite, we’re there for you." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_5_df4f1467ed.jpg 245w," sizes="100vw" width="1550" height="910">Maintain peak performance, minimize unexpected downtime, and ensure regulatory compliance of your AllianceTM iS HPLC System by partnering with Waters Service. Waters FlexCHOICETM Full-Service Plan for Regulated Laboratories includes:<ul style="list-style-type:disc;"><li>Yearly Waters Performance Maintenance</li><li>Priority* remote and onsite service including all parts, labor and travel</li><li>Full suite of compliance services</li><li>Discounts on future hardware upgrades and user training</li><li>Optional coverage available:<ul><li>Additional Performance Maintenance visits</li><li>Two business day on-site response time**</li></ul></li></ul>Priority over non-contract customers **May not be available in all areas<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_The_first_system_designed_for_Empower_the_Alliance_i_S_HPLC_System_features_intuitive_simplicity_built_specifically_for_QC_laboratories_112259fad6.jpg" alt="Waters Alliance iS HPLC System - The first system designed for Empower, the Alliance iS HPLC System features intuitive simplicity built specifically for QC laboratories." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_The_first_system_designed_for_Empower_the_Alliance_i_S_HPLC_System_features_intuitive_simplicity_built_specifically_for_QC_laboratories_112259fad6.jpg 234w," sizes="100vw" width="1024" height="682">

Thermo Scientific Vanquish Duo UHPLC Systems

A Vanquish Duo UHPLC system enables you to run two methods simultaneously on one instrument, doubling your throughput or deepening your sample knowledge while maximizing your productivity and performance.

<h3>Optimize your productivity</h3>In today’s challenging research and production environments, you are being asked to be more productive without increasing spending or compromising quality.&nbsp;At the same time, advancements and innovations are needed to improve operational efficiency and performance. How do you accomplish this? It’s easy with the Thermo Scientific™ Vanquish™ High-throughput LC systems built for enhanced productivity. Vanquish High-throughput LC systems combine individual Vanquish components and software wizards to deliver increased throughput or detector utilization.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_8_2befa2ab8e.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_8_2befa2ab8e.jpg 191w," sizes="100vw" width="1325" height="1086"><h3>Two flow paths for maximized productivity</h3>Your laboratory may be facing increased pressure to improve working efficiency and deal with limiting resources, such as budget, space, or manpower. Vanquish High-throughput LC systems are designed to overcome these limitations by providing multiple flow paths for parallelization of specific stages of the LC run.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_2_06370d93f8.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_2_06370d93f8.jpg 245w," sizes="100vw" width="955" height="591"><h4>Unique dual technology</h4>The Vanquish platform contains two unique dual modules. Vanquish dual pumps merge two individual, low pressure mixing pumps in one housing enabling separate eluents and gradients for each pump.Vanquish dual split samplers possess two independently controlled injection units including separate injection valves and injection needles. Used in Dual LC and LC-MS workflows only, this enables high throughput and extensive sample characterization workflows.<h3>Two configurations to best serve your needs</h3>Vanquish High-throughput workflows are configured with Thermo Scientific™ Vanquish™ Core system, Thermo Scientific™ Vanquish™ Flex system or Thermo Scientific™ Vanquish™ Horizon system modules. Both workflows make use of two pumps to increase instrument productivity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_3_77acff4bea.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems: Two configurations to best serve your needs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_3_77acff4bea.jpg 245w," sizes="100vw" width="1682" height="735"><h4>Vanquish Duo UHPLC Systems</h4><ul><li>Supported systems:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Vanquish Horizon</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/743">Vanquish Flex</a></li></ul></li><li>Sampler: Dual split sampler</li><li>Detector: Two out of the complete detector portfolio</li><li>Software control:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1838">Thermo Scientific™ Standard Instrument Integration (SII)</a> for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627">Waters™ Empower™ 3 CDS</a></li></ul></li></ul><h4>Vanquish Tandem LC Systems</h4><ul><li>Supported systems:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Vanquish Horizon</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/743">Vanquish Flex</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/397">Vanquish Core</a></li></ul></li><li>Sampler: Split sampler</li><li>Detector: One out of the complete detector portfolio and/or one mass spectrometer</li><li>Software control:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a></li><li>Standard System Integration for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758">Thermo Scientific™ Xcalibur™ software</a></li></ul></li></ul><h3>Vanquish Duo UHPLC systems</h3>A Vanquish Duo UHPLC system enables you to run two methods simultaneously on one instrument, doubling your throughput or deepening your sample knowledge while maximizing your productivity and performance. The unique Vanquish Duo UHPLC system will:<ul><li>Improve return on investment by reducing cost per sample</li><li>Increase sample throughput without changing validated methods</li><li>Increase capacity without sacrificing bench space</li><li>Accelerate your method development process</li><li>Analyze the same set of samples with multiple methods, reducing sample preparation time and maximizing sample knowledge</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_4_4cb62100fd.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_4_4cb62100fd.jpg 245w," sizes="100vw" width="1678" height="512"><h4>What can you do with a Vanquish Duo UHPLC system?</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_5_84912c40d4.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems: What can you do with a Vanquish Duo UHPLC system?" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_5_84912c40d4.jpg 245w," sizes="100vw" width="1604" height="658"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_6_f8939b888a.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems: Two independent assays run simultaneously from a single sample on one LC instrument to deepen your sample knowledge." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_6_f8939b888a.jpg 245w," sizes="100vw" width="1372" height="385"><h4>Complete control with just a few clicks</h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a> delivers industry-leading ease-of-use for the Vanquish Duo system. Independently control the two channels of the Vanquish Duo system from a single interface with full traceability of all actions that is 21 CFR Part 11 compliant.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_c2d9ed2c2c.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems: Easy-to-use and intuitive system control of the Dual LC workflow within Chromeleon CDS." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_c2d9ed2c2c.jpg 245w," sizes="100vw" width="1190" height="632"><h3>See the Vanquish Duo UHPLC system</h3>The Vanquish Duo UHPLC system has been built to utilize a wide range of analytical detectors, including mass spectrometry (MS), diode array detection (DAD), charged aerosol detection (CAD), variable wavelength detection (VWD), and fluorescence detection (FLD).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_7_c288dc2658.jpg" alt="Thermo Scientific Vanquish Duo UHPLC Systems: See the Vanquish Duo UHPLC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_7_c288dc2658.jpg 174w," sizes="100vw" width="939" height="841"><h5>1. Vanquish Solvent Monitor</h5>Use the Vanquish Solvent Monitor to increase your regulatory compliance, productivity, and safety of your laboratory. That device guarantees that your HPLC systems will never run dry and the waste will not spill over during your analysis, reducing loss of valuable measurement time and sample material.<h5>2. Vanquish User Interface</h5>With the Vanquish User Interface, you are the master of your LC instrument. The display always shows you the key parameters of your instrument such as flow and pressure and guides you in a visual step-by-step video tutorial through key maintenance tasks.<h5>3. Versatile detection capabilities</h5>Differing molecular characteristics of your analyte, from small molecules to complex biomolecules, necessitate multiple detector methodologies. Vanquish Duo UHPLC systems can be used with any two detectors out of the broad detector portfolio including MS, DAD, VWD, FLD and CAD.<h5>4. Compatible with your software infrastructure</h5>A Vanquish Duo UHPLC system can be operated in various software environments including the latest Chromeleon software feature release, Chromeleon CDS long-term, and extended support version and Waters Empower.<h5>5. Versatile column management</h5>Greater column capacity (two 30 cm columns or a greater number of shorter columns per compartment), plus class-leading temperature range and temperature stability.<h5>6. Delivering powerful separations</h5>Run samples with two similar or different columns to increase throughput or maximize output. Setup with optional second compartment for independent column temperatures.<h5>7. Easier operation</h5>Patented tool-free Thermo Scientific™ Viper™ Fingertight Fittings with near-zero dead volume operation and ergonomic design.<h5>8. Unique Thermo Scientific™ SmartInject technology</h5>Superior retention time precision and enhanced column protection with intelligent SmartInject technology on both flow paths.<h5>9. Two proprietary injection valves</h5>Two separate injection valves with no shared fluidics allowing full application flexibility, including individual eluents, wash liquids and loop sizes.<h5>10. Easier sample handling</h5>Automated barcode reader to eliminate tedious rack configuration.<h5>11. Higher sample capacity</h5>High sample capacity is standard (four plates versus two plates on industry average). Increase capacity further with the optional Thermo Scientific™ Vanquish™ Charger module.<h5>12. Accurate flow for more data confidence</h5>The flow is delivered by a dual pump offering two truly individual devices in a single housing with ternary gradient proportioning.<h5>13. Simple maintenance</h5>Module maintenance is reduced to a minimum and can be worked on without instrument de-stacking, plus smart light bars on the front of the system provide instant system status update.<h5>14. Keep the lab tidy</h5>The built-in drawer stores accessories, related documents, or any other useful material for the system.<h3>Thermo Scientific LC portfolio overview</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_LC_portfolio_overview_2db5044366.jpg" alt="Thermo Scientific LC portfolio overview." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_LC_portfolio_overview_2db5044366.jpg 188w," sizes="100vw" width="795" height="660">

Thermo Scientific Vanquish FLEX UHPLC System

Whether you are analyzing innovative biotherapeutics or novel chemical medicines, method development with Thermo Scientific™ Vanquish™ Flex™ UHPLC system has never been easier.

Thermo Scientific Vanquish Horizon UHPLC System

Designed with innovative technology and fine detail, the Vanquish Horizon UHPLC delivers outstanding precision, sensitivity, and simplicity for unmatched performance and robustness.

Products from category Liquid chromatographs - page 4

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