EU Regulation 396/2005: Pesticide Residue Testing and the Critical Role of QuEChERS Evaporation in Food Safety

<div><div>Researchers led by <a target="_blank" rel="noopener noreferrer" href="https://kudova.group.uochb.cz/en">Eva Kudová</a> from IOCB Prague and Jitka Viktorová from UCT Prague have identified new androstane-based steroid derivatives that can significantly improve the effectiveness of existing antibiotics against multidrug-resistant Staphylococcus aureus.</div></div><div>The team screened dozens of natural steroidal hormones and synthetic derivatives to see whether they could block bacterial efflux pumps – mechanisms that bacteria use to eject antibiotics and survive treatment. Two compounds emerged as particularly promising: they inhibited efflux pumps and lowered the effective doses of antibiotics such as ciprofloxacin and erythromycin.Further analysis showed that one of the compounds also suppressed genes linked to bacterial virulence. Another key finding is that the most active molecules are non-toxic to human immune cells and showed no detectable endocrine activity, suggesting a favorable safety profile.These findings support the idea that rationally designed steroid-based molecules could act as antibiotic adjuvants, helping existing drugs overcome bacterial resistance.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg" alt="IOCB Prague: Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_IOCB_Prague_Steroidal_hormones_and_neurosteroids_novel_therapeutic_strategies_in_bacterial_infections_8e5d2a7b48.jpg 222w," sizes="100vw" width="1258" height="886"><blockquote><h4>Original Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0223523426001613?via%3Dihub">Steroidal hormones and neurosteroids - novel therapeutic strategies in bacterial infections: Design, synthesis, and biological evaluation</a></h4>Daniela Brdová, Bára Křížkovská, Jan Špaček, Zdeněk Míchal, Eva Jablonská, Ondřej Strnad, Hana Chodounská, Eszter Szánti-Pintér, Marina Morozovová, Václav Hanžl, Jan Tkadlec, Martijn Riool, Jan Lipov, Jitka Viktorová, Eva Kudová&nbsp;Eur. J. Med. Chem., 2026, 308, 118716<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1016/j.ejmech.2026.118716">https://doi.org/10.1016/j.ejmech.2026.118716</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>The global rise of antibiotic resistance necessitates novel therapeutic strategies for infectious diseases. Inhibition of bacterial efflux pumps, which contribute to multidrug resistance, represents a promising approach to restore or even increase the efficacy of existing antibiotics. Using fluorescence-based ethidium bromide accumulation, broth microdilution, and checkerboard assays, we evaluated 26 endogenous steroidal hormones and neurosteroids, along with 30 synthetic derivatives, for their ability to enhance antibiotic susceptibility in multidrug-resistant Staphylococcus aureus. Structure-activity relationship analysis identified compounds 13 and 16 as lead candidates, exhibiting strong efflux pump inhibition and marked reductions in the minimum inhibitory concentrations of ciprofloxacin and erythromycin. Both compounds showed additive effects in checkerboard assays. Modifications at C-3 (polar substitution) and C-17 (3α,5β-stereochemistry and nonpolar substitution) were essential for potent efflux inhibition and sensitization, although these modifications were not additive when combined. Transcriptome analysis further revealed that compound 13 significantly downregulated S. aureus virulence-associated genes when administered alone or in combination with antibiotics. Cytotoxicity assessment in human peripheral blood mononuclear cells and receptor transactivation assays for estrogen, androgen, and progesterone receptors indicated that the most active derivatives were non-toxic and lacked detectable endocrine activity, suggesting a favorable safety profile. Overall, these findings support the concept that rationally designed andostane-based steroidal scaffolds can function as competitive bacterial efflux pump inhibitors and serve as potential antibiotic adjuvants to mitigate efflux-mediated resistance.<h4>Instrumentation</h4><h5>Structural characterization</h5><ul><li>AVANCE III 400 MHz NMR spectrometer (Bruker)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=LTQ+Orbitrap+XL">LTQ Orbitrap XL HRMS</a> (Thermo Fisher Scientific, ESI)</li></ul></div>

Steroidal hormones and neurosteroids – novel therapeutic strategies in bacterial infections

Metformin is currently the first-line oral drug of choice for the treatment of type 2 diabetes. It reduces glucose production in the liver, improves tissue sensitivity to insulin, and does not cause weight gain or hypoglycemia, which sets it apart from some other antidiabetic drugs. Moreover, its long-term beneficial effects on the risk of cardiovascular complications and mortality in people with type 2 diabetes have been well established. Researchers from the Institute of Physiology CAS (IPHYS) have identified a new group of blood metabolites that could help physicians objectively monitor whether patients with type 2 diabetes are taking their metformin as prescribed.<h4>New biomarkers for metformin use</h4>A team led by Tomáš Čajka from the Laboratory of Translational Metabolism at IPHYS analyzed plasma samples from 637 patients with type 2 diabetes treated with metformin and 143 non-diabetic controls using advanced metabolomics and lipidomics. Among 614 detected metabolites, they identified five from the group of N-lactoyl-amino acids (e.g. N‑lactoyl-phenylalanine), which had previously been seen as unknown signals due to missing reference spectra in databases. The levels of these metabolites in the blood closely reflected the plasma concentrations of metformin. In patients with the highest metformin exposure, the concentrations of these metabolites were up to 7.2 times higher than in controls.<h4>A sensitive “fingerprint” of the drug’s effect</h4>N-Lactoyl-amino acids are formed in cells by combining lactate and amino acids, thus reflecting the metformin-induced changes in cellular energy metabolism. “Importantly, even though they occur in plasma only at nanomolar concentrations, they reliably distinguish patients who take metformin regularly from those who take it irregularly or not at all,” emphasizes T. Čajka.<h4>Metabolomics in practice and international collaboration</h4>The study also provides a detailed description of broader metabolic changes in type 2 diabetes, including increases in lactate, organic acids, and branched-chain amino acids, as well as extensive remodelling of the lipid profile. In addition to T. Čajka’s team, collaborators from the Institute for Clinical and Experimental Medicine in Prague and the West Coast Metabolomics Center (University of California, Davis) also participated in the study.The results demonstrate that advanced metabolomics approaches developed at IPHYS within the CarDia project can find highly practical applications both in clinical studies and in personalized monitoring of treatment in patients with diabetes.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://ascpt.onlinelibrary.wiley.com/doi/10.1002/cpt.70205">Untargeted Metabolomics Identifies N-Lactoyl-Amino Acids as Dose-Responsive Plasma Biomarkers of Metformin Adherence in Type 2 Diabetes</a></h4>Tomas Cajka, Jiri Hricko, Lucie Rudl Kulhava, Michaela Paucova, Michaela Novakova, Veronika Hola, Stanislava Rakusanova, Oliver Fiehn, Vojtech Skop, Ivana Lankova, Iva Miskova, Terezie Pelikanova, Martin HaluzikClin Pharmacol Ther, 119: 1057-1069<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/cpt.70205">https://doi.org/10.1002/cpt.70205</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Metformin is the most widely prescribed antidiabetic drug, yet adherence remains difficult to objectively assess. Using untargeted metabolomics and lipidomics, we analyzed plasma from 637 patients with type 2 diabetes (T2D) with confirmed metformin use and 143 nondiabetic controls, annotating 614 metabolites. Patients were stratified by plasma metformin into sub-therapeutic, therapeutic, and supra-therapeutic groups, and associations were evaluated by multiple linear regression and composite metabolite ranking. Five previously unannotated features were structurally identified as N-lactoyl-amino acids, whose levels correlated strongly with plasma metformin (ρ = 0.42–0.55, P &lt; 0.0001) and increased up to 7.2-fold in the supra-therapeutic group (&gt; 2000 ng/mL). While N-lactoyl-amino acids were consistently detected in the nanomolar range, they still displayed robust and dose-dependent associations with metformin. Broader metabolic changes in T2D included elevated lactate, organic acids, and branched-chain amino acids, together with reduced urea cycle metabolites. Lipidomics showed increases in saturated triacylglycerols and diacylglycerols and decreases in cholesteryl esters, sphingomyelins, and phospholipids. These findings establish N-lactoyl-amino acids as robust, dose-responsive plasma biomarkers of metformin exposure. Despite being up to four orders of magnitude less abundant than their amino acid precursors, they sensitively reflect mitochondrial lactate overflow and pharmacodynamic adaptation, offering objective assessment of adherence.

New blood biomarkers reveal how faithfully diabetes patients take metformin

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The Czech Academy of Sciences (CAS) was established by Act No. 283/1992 Coll. as the Czech successor of the former Czechoslovak Academy of Sciences. It is set up as a complex of 54 public research institutions.

Czech Academy of Sciences

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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KNAUER Eurospher II 100 CN Prep HPLC Columns for NP & RP (USP L10)

CN is one of the most versatile stationary phases that can be used in reversed phase mode as well as in normal phase mode. 

Cyano or CN for Normal Phase and Reversed Phase (USP L10)CN is one of the most versatile stationary phases that can be used in reversed phase mode as well as in normal phase mode. The cyano modification supports the π-π interaction as well as polar and hydrophobic interactions with low polarity solvents. These phases are preferred for compounds with double bonds and aromatic groups. Cyano bonded phases show unique selectivity for polar compounds and are more suitable than bare silica for normal phase (NP) gradient separations. The cyano functional group is a strong dipole that can interact with other dipoles or induce dipoles on solutes. These phases also exhibit moderate hydrophobicity due to the alkyl linker.Due to the quick equilibration time of the CN bonded phase, it is the best choice for gradient elution in normal phase mode. CN phases can be used as a less polar alternative to Si phases.The material is typically preferred for:<ul><li>Compounds with double bonds</li><li>Cyclic antidepressants</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_CN_4fff7a1e28.webp" alt="KNAUER: CN" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_CN_4fff7a1e28.webp 130w," sizes="100vw" width="358" height="428"><blockquote>Stationary phase Eurospher II 100 CN is provided in these column types: analytical, semi-preparative and preparative.</blockquote>Eurospher II 100 CN Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 20, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul>Eurospher II 100 CN Preparative dAX Columns with dynamic axial compression are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 25, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul><h3>KNAUER Preparative HPLC Columns</h3>The right choice for all dimensions from semi-prep to batch chromatography to SMB.<ul><li>Modifications: Reversed Phase, HILIC, Normale Phase</li><li>Max. Pressure: 400–600 bar</li><li>Column Inner Diameter (ID): 16–50 mm in our shop, more on request</li><li>Column Brands: Eurospher, Eurospher II</li><li>Safe Investment: Robustness and durability based on superior technology</li><li>Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.</li><li>Customizable: Standard flanged hardware &amp; dAX hardware with static or dynamic axial compression available</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp" alt="KNAUER: KNAUER Preparative HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp 221w," sizes="100vw" width="1024" height="724"><h5>Preparative HPLC column hardware</h5>Preparative HPLC columns can be offered with two different hardware types:<ul><li>Versatile standard flanged hardware for nearly any application task in batch chromatography</li><li>dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes</li></ul>

KNAUER Eurospher II 100 Si Prep HPLC Columns for NP & HILIC (USP L3)

Unmodified Silica gel materials were the first ones used for chromatography. This is the reason the mode is called "normal phase" (NP).

Si columns for Normal Phase and HILIC applications (USP L3)Unmodified Silica gel materials were the first ones used for chromatography. This is the reason the mode is called "normal phase" (NP). They are still used in HPLC, although reversed phase (RP) is by far the most often used HPLC mode today.<h5>A wide range of porous Si materials are available, characterized by:</h5><ul><li>Surface area</li><li>Pore size</li><li>Particle size</li><li>Purity</li><li>Metal content</li></ul><h5>Properties</h5><ul><li>Separation mechanism: Hydrophilic interactions</li><li>Ultra pure, spherical high performance HPLC phase based on silica gel</li><li>No modification, no endcapping, with 0 % carbon content</li></ul><h5>Key features</h5><ul><li>High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, outstanding mechanical and chemical stability.</li></ul><h5>Recommended application areas</h5><ul><li>Wide range of different applications, i.e. SEC (size exclusion chromatography) but also for normal phase HPLC and HILIC; good choice for analytical and preparative purposes to separate polar compounds.</li></ul><blockquote>Tip: Normal phase (NP) stationary phases have been the first phases for chromatography and that is why their properties of polarity were determined as ’’normal’’. The stationary phase of a NP column has polar properties and is commonly used with nonpolar solvents as hexane or heptane. The other way round a reversed phase (RP) column has covalent charcteristics and is used with more polar eluents, like mixtures of acetonitrile and water.</blockquote>Stationary phase Eurospher II 100 Si is provided in these column types: analytical, semi-preparative and preparative.Eurospher II 100 Si Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 20, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul>Eurospher II 100 Si Preparative dAX Columns with dynamic axial compression are available with these parameters:<ul><li>Column length (mm): 50, 150, 250</li><li>Column ID (mm): 25, 30, 50</li><li>Particle size (µm): 5, 10, 15</li></ul><h3>KNAUER Preparative HPLC Columns</h3>The right choice for all dimensions from semi-prep to batch chromatography to SMB.<ul><li>Modifications: Reversed Phase, HILIC, Normale Phase</li><li>Max. Pressure: 400–600 bar</li><li>Column Inner Diameter (ID): 16–50 mm in our shop, more on request</li><li>Column Brands: Eurospher, Eurospher II</li><li>Safe Investment: Robustness and durability based on superior technology</li><li>Application: Easy upscaling, as all materials are available from ultra high performance to preparative scale HPLC. You can also use our upscaling calculator.</li><li>Customizable: Standard flanged hardware &amp; dAX hardware with static or dynamic axial compression available</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp" alt="KNAUER: KNAUER Preparative HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Preparative_HPLC_Columns_31bc2477c3.webp 221w," sizes="100vw" width="1024" height="724"><h5>Preparative HPLC columns can be offered with two different hardware types:</h5><ul><li>Versatile standard flanged hardware for nearly any application task in batch chromatography</li><li>dAX Hardware with static and dynamic axial compression for an extended lifetime and multi-column chromatography like SMB processes</li></ul>

KNAUER Eurospher II Chiral AM Prep Columns for NP & RP (USP L51)

Eurospher II Chiral AM uses the chiral selector Amylose-tris-(3,5 dimethylphenylcarbamate) and is equivalent to Chiralpak AD. 

Eurospher II Chiral AM uses the chiral selector Amylose-tris-(3,5 dimethylphenylcarbamate) and is equivalent to Chiralpak AD. KNAUER offers 2 different CPS versions: Eurospher II Chiral AM is designed for separations in normal phase (NP) mode and Eurospher II Chiral AM-R &nbsp;for separations in reversed phase (RP) mode.<h5>Properties</h5><ul><li>Silica gel: ultra pure, &gt; 99.99 %</li><li>Metal content: &lt; 10 ppm</li><li>Particle size: 3 μm, 5 μm, 10 μm</li><li>Particle form: spherical</li><li>Pore size of base material: 1000 Å</li><li>Chiral selector: Amylose-tris-(3,5-dimethylphenylcarbamate) for Chiral AM&nbsp;</li></ul><h5>Key features</h5><ul><li>KNAUER offers these chiral stationary phases for two different separation modes: Eurospher II Chiral AM is designed for separations in normal phase (NP) mode, Eurospher II Chiral AM-R for separations in reversed phase mode.</li></ul><h5>Recommended application areas</h5><ul><li>Eurospher II Chiral AM columns are able to separate neutral, acidic and basic racemates.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Chiral_AM_906d13b0b5.webp" alt="KNAUER: Eurospher II Chiral AM" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Chiral_AM_906d13b0b5.webp 245w," sizes="100vw" width="690" height="388"><blockquote>Stationary phase Eurospher II Chiral AM (AM-R) is provided in these column types: analytical, semi-preparative and preparative.</blockquote>Eurospher II Chiral AM and AM-R Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 150, 250</li><li>Column ID (mm): 20, 30</li><li>Particle size (µm): 5, 10</li></ul><h3>KNAUER Chiral HPLC Columns</h3>For analytical, preparative and SMB chiral separations<ul><li>Column Brand<ul><li>Eurospher II</li></ul></li><li>Reversed Phase Modifications<ul><li>Chiral NR-R</li><li>Chiral AM-R</li><li>Chiral OM-R</li></ul></li><li>Normal Phase Modifications<ul><li>Chiral NR</li><li>Chiral AM</li><li>Chiral OM</li></ul></li><li>Flexibility: Wide selection of column and particle sizes, for preparative and analytical applications</li><li>Expandable: Upscaling even for continuous SMB</li><li>Application Service: We offer a customized screening service for your analytes. You will receive an optimal column recommendation including the method parameters.</li></ul><h4>Eurospher II Chiral</h4>Based on ultra-high purity silica, the Eurospher II chiral column materials are characterized by high enantioselectivity and loadability.&nbsp;Available in a wide range of dimensions for analytical, preparative and SMB (simulated moving bed) applications, Eurospher II Chiral columns are the perfect choice for even the most challenging enantioseparation tasks&nbsp;Since chiral separations are not very well predictable, KNAUER offers a chiral screening service for your samples (Article number: AL0220).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Eurospher_II_chiral_48c53afebe.jpg" alt="KNAUER: Eurospher II chiral" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Eurospher_II_chiral_48c53afebe.jpg 245w," sizes="100vw" width="1369" height="633"><blockquote>Tip: Some of the most popular eluents for HPLC (such as acetone, chloroform, DMF, DMSO, MEK, toluene, dioxane, ethyl acetate, methylene chloride, pyridine and THF) which may be remaining in your HPLC system, can destroy Eurospher II Chiral AM and OM phases even in small concentrations! It is highly recommended that the HPLC system is flushed with appropriate eluents before the installation of the chiral column.</blockquote>

KNAUER Eurospher II Chiral OM Prep Columns for NP & RP (USP L40, USP L93)

Eurospher II Chiral OM uses the chiral selector Cellulose-tris-(3,5-dimethylphenylcarbamate) and is equivalent to Chiralcel OD. 

Eurospher II Chiral OM uses the chiral selector Cellulose-tris-(3,5-dimethylphenylcarbamate) and is equivalent to Chiralcel OD. KNAUER offers 2 different CPS versions: Eurospher II Chiral OM is designed for separations in normal phase (NP) mode and Eurospher II Chiral OM-R for separations in reversed phase (RP) mode.<h5>Properties</h5><ul><li>Silica gel: ultra pure, &gt; 99.99 %</li><li>Metal content: &lt; 10 ppm</li><li>Particle size: 3 μm, 5 μm, 10 μm</li><li>Particle form: spherical</li><li>Pore size of base material: 1000 Å</li><li>Chiral selector: Cellulose-tris-(3,5-dimethyl- phenylcarbamate) for Chiral OM</li></ul><h5>Key features</h5><ul><li>KNAUER offers these chiral stationary phases for two different separation modes: Eurospher II Chiral OM is designed for separations in normal phase (NP) mode, Eurospher II Chiral OM-R for separations in reversed phase mode.</li></ul><h5>Recommended application areas</h5><ul><li>Eurospher II Chiral OM columns are able to separate neutral, acidic and basic racemates.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Chiral_OM_c2a3f1a159.webp" alt="KNAUER: Eurospher II Chiral OM" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Chiral_OM_c2a3f1a159.webp 245w," sizes="100vw" width="690" height="388"><blockquote>Stationary phase Eurospher II Chiral OM (OM-R) is provided in these column types: analytical, semi-preparative and preparative.</blockquote>Eurospher II Chiral OM and OM-R Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 150, 250</li><li>Column ID (mm): 20, 30</li><li>Particle size (µm): 5, 10</li></ul><h3>KNAUER Chiral HPLC Columns</h3>For analytical, preparative and SMB chiral separations<ul><li>Column Brand<ul><li>Eurospher II</li></ul></li><li>Reversed Phase Modifications<ul><li>Chiral NR-R</li><li>Chiral AM-R</li><li>Chiral OM-R</li></ul></li><li>Normal Phase Modifications<ul><li>Chiral NR</li><li>Chiral AM</li><li>Chiral OM</li></ul></li><li>Flexibility: Wide selection of column and particle sizes, for preparative and analytical applications</li><li>Expandable: Upscaling even for continuous SMB</li><li>Application Service: We offer a customized screening service for your analytes. You will receive an optimal column recommendation including the method parameters.</li></ul><h4>Eurospher II Chiral</h4>Based on ultra-high purity silica, the Eurospher II chiral column materials are characterized by high enantioselectivity and loadability.&nbsp;Available in a wide range of dimensions for analytical, preparative and SMB (simulated moving bed) applications, Eurospher II Chiral columns are the perfect choice for even the most challenging enantioseparation tasks&nbsp;Since chiral separations are not very well predictable, KNAUER offers a chiral screening service for your samples (Article number: AL0220).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Eurospher_II_chiral_48c53afebe.jpg" alt="KNAUER: Eurospher II chiral" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Eurospher_II_chiral_48c53afebe.jpg 245w," sizes="100vw" width="1369" height="633"><blockquote>Tip: Some of the most popular eluents for HPLC (such as acetone, chloroform, DMF, DMSO, MEK, toluene, dioxane, ethyl acetate, methylene chloride, pyridine and THF) which may be remaining in your HPLC system, can destroy Eurospher II Chiral AM and OM phases even in small concentrations! It is highly recommended that the HPLC system is flushed with appropriate eluents before the installation of the chiral column.</blockquote>

KNAUER Eurospher II Chiral NR Prep Columns for NP & RP

Eurospher II Chiral NR is an immobilized brush-type phase with very broad generality and orhogonal selectivity to Eurospher II Chiral AM and OM.

Eurospher II Chiral NR is an immobilized brush-type phase with very broad generality and orhogonal selectivity to Eurospher II Chiral AM and OM. In many cases it is much more stable compared to Eurospher II Chiral AM or OM. The Chiral-NR selector is covalently bound, so that you can use all HPLC-eluents.Due to its high loadability it is perfectly suited for preparative separations. Both phases can be used in RP and NP mode. Eurospher II Chiral-NR-R shows reversed elution order compared to Eurospher II Chiral-NR caused by the modification with the opposite enantiomeric form of the chiral selector.<h5>Properties</h5><ul><li>Silica gel: ultra pure, &gt; 99.99 %</li><li>Metal content: &lt; 10 ppm</li><li>Particle size:&nbsp;<ul><li>Eurospher II Chiral NR: 3 μm, 5 μm, 8 µm, 12 µm</li><li>Eurospher II Chiral NR-R: 8 µm, 12 µm</li></ul></li><li>Particle form: spherical</li><li>Pore size of base material: 1000 Å</li></ul><h5>Recommended application areas</h5><ul><li>Eurospher II Chiral NR columns are able to separate analytes with an oxygen at or near the chiral center and additionally an aromatic ring.</li></ul><blockquote>Stationary phase Eurospher II Chiral NR (NR-R) is provided in these column types: analytical, semi-preparative and preparative.</blockquote>Eurospher II Chiral NR and NR-R Preparative Standard Columns are available with these parameters:<ul><li>Column length (mm): 150, 250</li><li>Column ID (mm): 20, 30</li><li>Particle size (µm): 8, 12</li></ul><h3>KNAUER Chiral HPLC Columns</h3>For analytical, preparative and SMB chiral separations<ul><li>Column Brand<ul><li>Eurospher II</li></ul></li><li>Reversed Phase Modifications<ul><li>Chiral NR-R</li><li>Chiral AM-R</li><li>Chiral OM-R</li></ul></li><li>Normal Phase Modifications<ul><li>Chiral NR</li><li>Chiral AM</li><li>Chiral OM</li></ul></li><li>Flexibility: Wide selection of column and particle sizes, for preparative and analytical applications</li><li>Expandable: Upscaling even for continuous SMB</li><li>Application Service: We offer a customized screening service for your analytes. You will receive an optimal column recommendation including the method parameters.</li></ul><h4>Eurospher II Chiral</h4>Based on ultra-high purity silica, the Eurospher II chiral column materials are characterized by high enantioselectivity and loadability.&nbsp;Available in a wide range of dimensions for analytical, preparative and SMB (simulated moving bed) applications, Eurospher II Chiral columns are the perfect choice for even the most challenging enantioseparation tasks&nbsp;Since chiral separations are not very well predictable, KNAUER offers a chiral screening service for your samples (Article number: AL0220).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Eurospher_II_chiral_48c53afebe.jpg" alt="KNAUER: Eurospher II chiral" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Eurospher_II_chiral_48c53afebe.jpg 245w," sizes="100vw" width="1369" height="633"><blockquote>Tip: Some of the most popular eluents for HPLC (such as acetone, chloroform, DMF, DMSO, MEK, toluene, dioxane, ethyl acetate, methylene chloride, pyridine and THF) which may be remaining in your HPLC system, can destroy Eurospher II Chiral AM and OM phases even in small concentrations! It is highly recommended that the HPLC system is flushed with appropriate eluents before the installation of the chiral column.</blockquote>

Phenomenex Luna Omega Polar C18 HPLC Prep Columns

Luna Omega Polar C18 is a novel HPLC and UHPLC stationary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non polar analytes.

Reversed Phase Polar Retention Without the HeadacheLuna Omega Polar C18 is a novel HPLC and UHPLC stationary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non polar analytes. The all purpose C18 ligand provides hydrophobic interactions while a polar modified particle surface provides enhanced polar retention and also aqueous stability. These attributes make the Luna Omega Polar C18an excellent choice for balanced retention of polar and hydrophobic compounds as well as to solely enhance retention of highly polar compounds.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems</li><li>Fully Scalable Particle Size UHPLC HPLC Preparative</li><li>100 % Aqueous Stable C18 Selectivity</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_Omega_Polar_C18_HPLC_Column_a9c42ff59a.jpg" alt="Phenomenex: Luna Omega Polar C18 HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_Omega_Polar_C18_HPLC_Column_a9c42ff59a.jpg 245w," sizes="100vw" width="870" height="315"><ul><li>Carbon Load: null %, 11.0 %</li><li>Particle Size: 1.6, 3, 5</li><li>Recommended Use: Polar retentive C18 phase for HPLC and UHPLC separations of hydrophobic/polar compounds</li></ul><h4>Not Just Another C18…Built Different</h4>Luna Omega Polar C18 is a uniquely modified C18 based chemistry that has been optimized to improve the performance of polar analyses. This new particle surface chemistry makes the Polar C18 applicable to all industries that utilize HPLC for mixtures of polar and non polar compounds.<h4>Stable Retention &amp; 100% Aqueous Stable&nbsp;</h4>Traditional C18 phases are known to collapse under 100 % aqueous conditions, causing retention loss of compounds and a method development headaches. That is why an advanced proprietary bonding technology was used for the Luna Omega Polar C18 in order to ensure aqueous stability.&nbsp;<h4>Increased Reversed Phase Polar Retention</h4>With its polar modified surface, the Luna Omega Polar C18 offers UHPLC users enhanced separation power that can greatly improve resolution values for target compounds.<blockquote>Stationary phase Luna Omega Polar C18 is provided in these column types: analytical, microbore, minibore, midbore and preparative</blockquote><h5>Luna Omega Polar C18 HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna Omega HPLC/UHPLC Columns</h3>The cutting edge thermally modified Luna Omega particle delivers high efficiency, ruggedness, reproducibility, and dependability for a wide range of chromatographic analyses. Luna Omega HPLC and UHPLC columns are built upon more then 20 years of applied knowledge, invention, and customer experience in the manufacture and packing of LC columns. The particle’s novel design and proprietary manufacturing process results in greater particle inertness, a stronger particle morphology, and more consistent porosity.<ul><li>Greater separation muscle and retention</li><li>Consistent porous structure and elimination of micropores</li><li>Extreme ruggedness and reproducibility</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Thermal_modified_pore_structure_of_Luna_Omega_Column_particles_37138bb135.jpg" alt="Phenomenex:Thermal modified pore structure of Luna Omega Column particles" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Thermal_modified_pore_structure_of_Luna_Omega_Column_particles_37138bb135.jpg 245w," sizes="100vw" width="1257" height="369"><h3>Explore Luna Omega Columns</h3><h5>Astounding Performance</h5>The undeniably high efficiency levels found in each Luna Omega UHPLC column provide you with the potential of huge gains in method performance. While traditional silica and hybrid fully porous particles claim high performance, when compared to Luna Omega 1.6 μm, they drastically fall short and prevent UHPLC scientists from reaching their UHPLC potential.<h5>Separation Muscle</h5>Our industry leading bonding technologies in conjunction with high efficiency levels ensure excellent stationary phase coverage and improved separation power. Now, with Luna Omega 1.6 μm, you can turn difficult separations into resolution achievements.<h5>Inert Foundation</h5>Luna Omega UHPLC columns contain a unique silica modified using a proprietary, post-synthetic thermal treatment process to provide extraordinary mechanical strength and significantly greater inertness than traditional fully porous and hybrid materials. This greatly minimizes secondary interactions that negatively affect peak shape, allowing for greater method accuracy.<h5>Consistent Results</h5>Batch-to-batch and column-to-column, Luna® Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure dependability and reproducibility.

Phenomenex Luna Omega PS C18 HPLC Prep Columns

Luna Omega PS C18 is a unique mixed interaction stationary phase that provides incredibly useful polar and non-polar retention. 

Improve Peak Shape for Bases Without the HeadacheLuna Omega PS C18 is a unique mixed interaction stationary phase that provides incredibly useful polar and non-polar retention. The surface of the PS C18 contains a positive charge which aids in the retention of acidic compounds and provides better peak shape for bases through ionic interactions, while the C18 ligand promotes general reversed phase retention. This mixed-mode selectivity allows for greater separation between compounds with varying functional groups.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems</li><li>Fully Scalable Particle Size UHPLC HPLC Preparative</li><li>Charged Surface C18 that is 100 % Aqueous Stable</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_Omega_PS_C18_HPLC_Column_bf5cf29276.webp" alt="Phenomenex: Luna Omega PS C18 HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_Omega_PS_C18_HPLC_Column_bf5cf29276.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: null %, 11.0 %</li><li>Particle Size: 1.6, 3, 5</li><li>Recommended Use: Positive surface C18 phase for HPLC and UHPLC separations of hydrophobic/basic compounds</li></ul><h4>A C18 With a Positive Twist</h4>Luna Omega PS C18 has been fine tuned and manufactured by Phenomenex to provide a mixed selectivity that is highly useful for method development involving either combinations of polar and nonpolar compounds, or just one single compound class with small changes in functional groups.<h4>Enhanced Selectivity and Polar Retention</h4>With its positive surface (PS) the Luna Omega PS C18 provides valuable increase in retention of acids through ionic/polar interactions. In addition, the C18 ligand also stimulates hydrophobic retention that greatly promotes increased resolution between analytes of interest.<h4>Think Outside the Traditional C18 Selectivity</h4>Traditional C18 phases may not always be the best option. A HPLC and UHPLC column that has both polar and hydrophobic versatility allows for great method development flexibility.<h4>Better Peak Shape for Bases</h4>While traditional alkyl phases are prone to show tailing for basic compounds because of secondary interactions occurring at the silica surface, the surface of the Luna Omega PS C18 was designed with positive charges that serve to repel strong basic species and consistently display sharp peak shape.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Better_Peak_Shape_for_Bases_Comparison_of_Luna_Omega_PS_C18_with_C18_Columns_from_other_manufacturers_3667109291.jpg" alt="Phenomenex: Better Peak Shape for Bases (Comparison of Luna Omega PS C18 with C18 Columns from other manufacturers)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Better_Peak_Shape_for_Bases_Comparison_of_Luna_Omega_PS_C18_with_C18_Columns_from_other_manufacturers_3667109291.jpg 245w," sizes="100vw" width="905" height="485"><blockquote>Stationary phase Luna Omega PS C18 is provided in these column types: analytical, minibore, midbore and preparative</blockquote><h5>Luna Omega PS C18 HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna Omega HPLC/UHPLC Columns</h3>The cutting edge thermally modified Luna Omega particle delivers high efficiency, ruggedness, reproducibility, and dependability for a wide range of chromatographic analyses. Luna Omega HPLC and UHPLC columns are built upon more then 20 years of applied knowledge, invention, and customer experience in the manufacture and packing of LC columns. The particle’s novel design and proprietary manufacturing process results in greater particle inertness, a stronger particle morphology, and more consistent porosity.<ul><li>Greater separation muscle and retention</li><li>Consistent porous structure and elimination of micropores</li><li>Extreme ruggedness and reproducibility</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Thermal_modified_pore_structure_of_Luna_Omega_Column_particles_37138bb135.jpg" alt="Phenomenex:Thermal modified pore structure of Luna Omega Column particles" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Thermal_modified_pore_structure_of_Luna_Omega_Column_particles_37138bb135.jpg 245w," sizes="100vw" width="1257" height="369"><h3>Explore Luna Omega Columns</h3><h5>Astounding Performance</h5>The undeniably high efficiency levels found in each Luna Omega UHPLC column provide you with the potential of huge gains in method performance. While traditional silica and hybrid fully porous particles claim high performance, when compared to Luna Omega 1.6 μm, they drastically fall short and prevent UHPLC scientists from reaching their UHPLC potential.<h5>Separation Muscle</h5>Our industry leading bonding technologies in conjunction with high efficiency levels ensure excellent stationary phase coverage and improved separation power. Now, with Luna Omega 1.6 μm, you can turn difficult separations into resolution achievements.<h5>Inert Foundation</h5>Luna Omega UHPLC columns contain a unique silica modified using a proprietary, post-synthetic thermal treatment process to provide extraordinary mechanical strength and significantly greater inertness than traditional fully porous and hybrid materials. This greatly minimizes secondary interactions that negatively affect peak shape, allowing for greater method accuracy.<h5>Consistent Results</h5>Batch-to-batch and column-to-column, Luna® Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure dependability and reproducibility.

Phenomenex Luna HILIC HPLC Preparative Columns

The Luna HILIC is a diol based silica phase that allows for an denser aqueous layer for increase polar selectivity.

The Luna HILIC is a diol based silica phase that allows for an denser aqueous layer for increase polar selectivity.<ul><li>Reproducible‚ robust HILIC separations</li><li>Made for retention of polar compounds</li><li>Increase mass spectrometry sensitivity</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_HILIC_HPLC_Column_4b955cbfde.webp" alt="Phenomenex: Luna HILIC HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_HILIC_HPLC_Column_4b955cbfde.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 5.7, 5.7 %</li><li>Surface Area (m²/g): 200</li><li>Particle Size: 3, 5</li><li>Recommended Use: Diol phase useful for hydrophilic interaction chromatography mode</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 (= 99.99 %) purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Increase MS Sensitivity and Retention for Polar Compounds</h4>Hydrophilic Interaction Liquid Chromatography (HILIC) is a separation mode where the partitioning of polar solutes from the high concentration‚ water-miscible‚ organic mobile phase into the hydrophilic surface environment creates separations. Polar solutes exhibit increased retention and elute in the order of increasing hydrophilicity.<h4>Common Applications:</h4><ul><li>Drug metabolites, Water soluble vitamins, Melamine, Cyanuric acid, Metanephrine, Normetanephrine</li></ul><blockquote>Stationary phase Luna HILIC is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna HILIC HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Luna Phenyl-Hexyl HPLC Preparative Columns

The Luna Phenyl Hexyl is our most hydrophobic phenyl column and it will also provide good hydrogen accepting functionality for acidic retention.

The Luna Phenyl Hexyl is our most hydrophobic phenyl column and it will also provide good hydrogen accepting functionality for acidic retention.<ul><li>Extensive line of rugged particle sizes</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>Phenyl-Hexyl Ultra-pure, metal-free silica (99.99% purity) (USP: L11)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_phenyl_hexyl_HPLC_Column_e0aa55de27.jpg" alt="Phenomenex: Luna phenyl-hexyl HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_phenyl_hexyl_HPLC_Column_e0aa55de27.jpg 245w," sizes="100vw" width="599" height="309"><ul><li>Carbon Load: 17.5, 17.5 %</li><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5, 10, 15</li><li>Recommended Use: Separation of aromatic and closely related compounds</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Engineered for Stability</h4>Luna Phenyl-Hexyl columns provide separations not achievable on C18 or C8 columns; such as increased retention for polar‚ aromatic compounds as well as reversals in analyte elution order. Luna Phenyl-Hexyl columns are a reproducible‚ extremely stable phenyl phase.<h4>Common Applications:</h4><ul><li>Oxacillin, Captopril, Chlorpheniramine, Pseudoephedrine, Methadone Hydrochloride Oral Concentration</li></ul><blockquote>Stationary phase Luna Phenyl-Hexyl is provided in these column types: analytical, microbore, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna Phenyl-Hexyl HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5, 10</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Phenomenex Luna CN HPLC Preparative Columns

The Luna CN has Nitrile groups bound to the silica surface which offer a unique polar selectivity under reversed phase or normal phase conditions.

The Luna CN has Nitrile groups bound to the silica surface which offer a unique polar selectivity under reversed phase or normal phase conditions.<ul><li>Extensive line of rugged particle sizes</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>CN Ultra pure, metal-free silica (99.99% purity) (USP:L10)</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_CN_HPLC_Column_114efce581.webp" alt="Phenomenex: Luna CN HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_CN_HPLC_Column_114efce581.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 7.7 %</li><li>Surface Area (m²/g): 400</li><li>Particle Size: 3, 5, 10</li><li>Recommended Use: HILIC or normal phase separation of extremely polar compounds or when a cyano phased or L10</li></ul><h4>Long Column Lifetimes and Excellent Performance</h4>Ultra-pure, metal-free silica (99.99% purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.<h4>Proven Reproducibility</h4>For carboxyl‚ carbonyl‚ and amine containing compounds‚ Luna CN columns offer a unique polar selectivity in reversed phase and normal phase modes. Luna CN columns provide sharp peaks and great reproducibility run-to-run‚ column-to-column and batch-tobatch. State of the art modification of the silca surface ensures improved resistance to bonded phase hydrolysis providing one of the most stable CN phases on the market. The result:<ul><li>Excellent polar selectivity</li><li>Improved peak shapes</li><li>One of the most stable CN columns under reversed phase or normal phase conditions</li><li>pH stable from 1.5 to 7.0</li></ul><h5>Common Applications:</h5><ul><li>Benzalkonium Chloride, Nortriptyline HCl Capsules, Prednisolone, Tetracaine, Quinapril tablets</li></ul><blockquote>Stationary phase Luna CN is provided in these column types: analytical, minibore, midbore, semi-preparative and preparative</blockquote><h5>Luna CN HPLC Axia™ Packed Preparative Columns are available with these parameters:</h5><ul><li>Column length (mm): 30, 50, 100, 150, 250</li><li>Column ID (mm): 21.2; 30</li><li>Particle size (µm): 5, 10</li></ul><h3>Phenomenex Luna HPLC Columns</h3>Luna HPLC columns are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by Phenomenex, Luna HPLC columns are made from 99.999% ultra-pure, metal-free silica, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of chromatographic applications, from microbore and fast LC to preparative and bulk purification scales.All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.<h4>Explore Luna Columns</h4><ul><li>Proven Performance: Extensive line of rugged USP and Ph.Eur. phases</li><li>Maximum Reproducibility: Each column has both a certificate of analysis and a certificate of quality assurance</li><li>Ultra-High Purity: Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li>Scalable: Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul>Explore resolution with 10 unique Luna selectivities and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490">

Products focused on Consumables - page 18

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