<div><div><div>Scientists at IOCB Prague have developed a breakthrough technology that streamlines aptamer development. The new method integrates several biochemical and bioinformatics steps and paves the way for new diagnostic and therapeutic applications. Ongoing applications include wearable sensors that monitor selected biomarkers in real time and provide early warnings of impending health complications. The findings were published in Nature Communications.</div></div><div>Aptamers are short DNA or RNA strands that can recognize and bind to a specific target molecule with high precision. Similar to antibodies, they can be used to detect these molecules or modulate their activity. Unlike antibodies, they are much more stable, can be produced synthetically, and can be chemically modified to achieve the desired properties. As a result, they can offer capabilities that cannot be achieved with antibodies.</div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg" alt="IOCHB Prague: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Chemically modified DNA aptamer bound to the target protein." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_2_81e99b9900.jpg 228w," sizes="100vw" width="1343" height="916">As demand grows for accurate and rapid diagnostic tools, aptamers are often better suited to these applications than antibodies. However, developing aptamers is both experimentally demanding and time-consuming. A team of scientists from IOCB Prague, led by Dr. Marek Ondruš and <a target="_blank" rel="noopener noreferrer" href="https://hocek.group.uochb.cz/en">Prof. Michal Hocek</a>, has now developed a technology that significantly shortens the development process.“Developing an aptamer is often like looking for a needle in a haystack. Among trillions of different DNA sequences, we search for the one that binds strongly and specifically to a target molecule. Our new approach first screen for ‘aptamer families’ – hundreds of related sequences – and then identify the best-performing member of the family. This approach can reduce development time from several months to only a few days,” says Marek Ondruš.<blockquote>Video:<a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=_3zXUeVMugU"> New technology streamlines the development of aptamers, synthetic alternatives to antibodies&nbsp;</a></blockquote>The team has also expanded the potential applications of aptamers by chemically modifying their building blocks. For example, introducing functional groups similar to those found naturally in amino acids allows the resulting molecules to combine the advantages of both DNA and antibodies: Their sequence is encoded by the order of nucleotides in DNA, while the attached chemical modifications greatly expand their ability to recognize biologically relevant targets and mimic interactions between proteins that are underlying many biological processes.The researchers demonstrated the potential of this new method using the human insulin receptor, a key protein that regulates blood sugar levels. They developed an aptamer that specifically binds to the receptor and elucidated the molecular basis of this interaction using cryo-electron microscopy. “We have shown that chemically introduced functional groups play a crucial role not only in target recognition and formation of the aptamer–protein complex, but also in stabilizing the DNA aptamer’s structure. Our findings provide new insights into how chemically modified nucleic acids can mimic key properties of proteins,” says Marek Ondruš.In the future, the technology could complement – or, in some applications, even replace – antibody-based diagnostic tools. Researchers are collaborating with <a target="_blank" rel="noopener noreferrer" href="http://iocbtech.cz/">IOCB Tech</a> on its further development, as the company helps to bring discoveries from IOCB Prague into practical applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg" alt="IOCHB Prague/Tomáš Belloň: New technology streamlines the development of aptamers, synthetic alternatives to antibodies: Dr. Marek Ondruš, first author of the study, and Prof. Michal Hocek, head of the Bioorganic and Medicinal Chemistry of Nucleic Acids research group at IOCB Prague." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nova_technologie_inovuje_vyvoj_aptameru_syntetickych_alternativ_protilatek_9694c21671.jpg 234w," sizes="100vw" width="2000" height="1335"><blockquote><h4>Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-73676-y#citeas">Expedient single-round selection of hyper-modified aptamer targeting insulin receptor from over-represented dually nucleobase-modified DNA libraries</a></h4>ablo Alberto Franco-Urquijo, Marek Ondruš, Jaroslav Kurfürst, Jana Škerlová, Irena Selicharová, Lucie Mužíková Čechová, Hana Šváchová, Alena Semerádtová, Anatolij Filimoněnko, Adéla Fejfarová, Jiří Homola, Tomáš Kouba &amp; Michal HocekNat. Commun., 2026<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1038/s41467-026-73676-y">https://doi.org/10.1038/s41467-026-73676-y</a>&nbsp;<h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Discovery of functional nucleic acids from randomized libraries typically relies on multiple, time-consuming iterative rounds of in vitro selection with low success rate. Here, we present a single-round selection strategy for rapid screening of multiple over-represented nucleobase-modified DNA libraries and various selection conditions, capable of identifying high-affinity modified aptamers. Double partition followed by amplification of eluted sequences, NGS analysis and clustering provides fast identification of aptamer candidates. Screening of modified DNA libraries containing modified adenine and uracil nucleotides bearing hydrophobic aromatic phenyl and indole moieties results in development of an aptamer binding human insulin receptor with sub-nanomolar affinity and exquisite specificity. Cryo-EM structure reveals the importance of each aromatic modification, either in stabilizing the secondary structure or facilitating interactions with the protein surface. This approach addresses the main drawbacks of aptamer selection and has potential for high-throughput screening and accelerating the development of next-generation aptamers for diagnostics or therapeutics.</div>

New technology streamlines the development of aptamers, synthetic alternatives to antibodies

Clarity 10.2 expands instrument control capabilities with new control modules and updates the MS extension to help you work more efficiently.<h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity</a> 10.2 highlights</h4><ul><li>New and updated control modules</li><li>Improved MS data import and export</li><li>Obsolete hardware support ended</li></ul><h4>Instrument control modules</h4>Clarity 10.2 introduces new instrument control modules from Annuo (AS-3117, FAS-3823) and updated instrument controls form Sykam and Ecom.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Instrument_control_modules_5de26782b0.jpg" alt="DataApex: Instrument control modules" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Instrument_control_modules_5de26782b0.jpg 228w," sizes="100vw" width="380" height="260"><h4>MS data import/export improvements</h4>Clarity improves MS data exchange, especially for *.cdf files. MS instruments now support export and import of multiple spectral data and non-spectral signal data in the same CDF file. Import also better handles uniform sampling information, m/z Step values, scale factors, and file names with non-ASCII characters. Exporting individual chromatogram signals from MS instruments is now available through a separate Export Chromatogram dialog.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg" alt="DataApex: MS data import-export improvements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_MS_data_import_export_improvements_a7967a4020.jpg 228w," sizes="100vw" width="378" height="259"><h4>Obsolete hardware support terminated</h4>Due to changes in Windows driver security requirements, this update discontinues support for obsolete hardware, including INT7, INT9, U-PAD, U-PAD2, and CB20.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg" alt="DataApex: Obsolete hardware support terminated" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Data_Apex_Obsolete_hardware_support_terminated_24d9329419.jpg 228w," sizes="100vw" width="378" height="259"><blockquote>Explore the complete list of all changes, fixes, and additions in version 10.2.👉<a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">See the complete changelog&nbsp;</a></blockquote>

Clarity Chromatography Software 10.2 released!

🎤Dr. Leigh Terry (University of Alabama)In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.<h4>In this episode, we cover:</h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted LC-MS/MS methods</li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (CIC) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4>Key Insight:</h4>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.<h3>Video Transcription</h3>In this episode of Concentrating on Chromatography, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.<h4>Understanding PFAS and Their Significance</h4><h5>The University of Alabama Water Quality Research Center</h5>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.The laboratory specializes in:<ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.<h4>Why Focus on PFAS?</h4>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:<ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.<h4>The Development of Standardized PFAS Methods</h4>One major advancement in PFAS analysis has been the development of standardized EPA methods.These methods allow:<ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.<h4>How Large Is the PFAS Universe?</h4>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.PFOA and PFOS are often referred to as:<ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.Researchers estimate:<ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul>Scientific debate continues regarding classification and definition, making precise counting difficult.<h4>Why PFAS Are Called "Forever Chemicals"</h4>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.PFAS persistence results from two key chemical properties.<h5>1. Amphiphilic Structure</h5>PFAS molecules contain:<ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul>This unusual combination allows PFAS to partition between:<ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.<h5>2. Extremely Strong Carbon–Fluorine Bonds</h5>The carbon–fluorine bond is among the strongest bonds in organic chemistry.As Dr. Terry explains:<ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul>These properties make PFAS extremely persistent in both environmental systems and biological organisms.<h4>Toxicity, Regulations, and Environmental Behavior</h4><h5>Understanding PFAS Toxicity</h5>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.Dr. Terry explains that EPA regulations generally consider three factors:<ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.This creates important scientific questions:<ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul>These uncertainties continue to drive active research.<h4>What Happens to PFAS in Wastewater Treatment Plants?</h4>PFAS do not behave like many conventional contaminants.Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:<ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul>As a result:<ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul>Their distribution depends on wastewater composition and treatment processes.<h4>Regulatory Monitoring and Targeted LC-MS Analysis</h4>Current EPA regulations focus on targeted analysis of specific PFAS compounds.Examples include:<ul><li>PFOA</li><li>PFOS</li></ul>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.Advantages of targeted LC-MS analysis include:<ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul>However, limitations remain:<ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul>As Dr. Terry notes, targeted analysis provides only part of the overall picture.<h4>The Fluorine Mass Balance Problem</h4>One of the most important concepts discussed in the interview is fluorine mass balance.When PFAS degrade, fluorinated fragments may remain in the environment.This creates a critical question:Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?To answer this, researchers perform fluorine mass balance calculations using:<ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.<h4>Advanced Analysis, Treatment Technologies, and Future Directions</h4><h5>Combining Targeted and Bulk Fluorine Measurements</h5>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.Approaches include:<ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul>In these methods:<ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.<h4>Surprising Findings from Combined Analysis</h4>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.Dr. Terry reports that:<ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.<h4>Preventing PFAS Contamination in the Laboratory</h4>PFAS analysis requires exceptionally strict quality control.Dr. Terry's laboratory routinely:<ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.<h4>Current PFAS Treatment Technologies</h4>Three technologies currently dominate PFAS removal in drinking water treatment:<ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul>Selection depends on:<ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.<h4>Membrane Filtration and Pretreatment</h4>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.Her own work focuses on pretreatment through biological filtration.The strategy involves:<ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.<h4>Regional Monitoring Projects</h4>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.Their observations indicate:<ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul>Examples include:<ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul>Source identification remains a critical component of environmental monitoring programs.<h4>Future Directions for PFAS Monitoring</h4>Looking ahead, Dr. Terry sees several opportunities for improvement.Desired advances include:<ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.<h4>Dr. Terry's Perspective on Long-Term Solutions</h4>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.Rather than focusing solely on remediation, she emphasized prevention.Her view is that society should:<ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.<h4>Final Thoughts</h4>Dr. Terry encourages consumers to:<ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.<h4>Conclusion</h4>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

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Environment

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Science and research

Software

Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

DataApex

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Thermo Scientific Vanquish Tandem LC Systems

The Thermo Scientific Vanquish Tandem LC Systems increase throughput and maximize detector output by using a second pump and intelligent column switching to eliminate the time required for column equilibration in a gradient elution. 

<h3>Optimize your productivity</h3>In today’s challenging research and production environments, you are being asked to be more productive without increasing spending or compromising quality.&nbsp;At the same time, advancements and innovations are needed to improve operational efficiency and performance. How do you accomplish this? It’s easy with the Thermo Scientific™ Vanquish™ High-throughput LC systems built for enhanced productivity. Vanquish High-throughput LC systems combine individual Vanquish components and software wizards to deliver increased throughput or detector utilization.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_8_2befa2ab8e.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_8_2befa2ab8e.jpg 191w," sizes="100vw" width="1325" height="1086"><h3>Two flow paths for maximized productivity</h3>Your laboratory may be facing increased pressure to improve working efficiency and deal with limiting resources, such as budget, space, or manpower. Vanquish High-throughput LC systems are designed to overcome these limitations by providing multiple flow paths for parallelization of specific stages of the LC run.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_2_06370d93f8.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_2_06370d93f8.jpg 245w," sizes="100vw" width="955" height="591"><h4>Unique dual technology</h4>The Vanquish platform contains two unique dual modules. Vanquish dual pumps merge two individual, low pressure mixing pumps in one housing enabling separate eluents and gradients for each pump.Vanquish dual split samplers possess two independently controlled injection units including separate injection valves and injection needles. Used in Dual LC and LC-MS workflows only, this enables high throughput and extensive sample characterization workflows.<h3>Two configurations to best serve your needs</h3>Vanquish High-throughput workflows are configured with Thermo Scientific™ Vanquish™ Core system, Thermo Scientific™ Vanquish™ Flex system or Thermo Scientific™ Vanquish™ Horizon system modules. Both workflows make use of two pumps to increase instrument productivity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_3_77acff4bea.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems: Two configurations to best serve your needs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Duo_UHPLC_Systems_3_77acff4bea.jpg 245w," sizes="100vw" width="1682" height="735"><h4>Vanquish Duo UHPLC Systems</h4><ul><li>Supported systems:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Vanquish Horizon</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/743">Vanquish Flex</a></li></ul></li><li>Sampler: Dual split sampler</li><li>Detector: Two out of the complete detector portfolio</li><li>Software control:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1838">Thermo Scientific™ Standard Instrument Integration (SII)</a> for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627">Waters™ Empower™ 3 CDS</a></li></ul></li></ul><h4>Vanquish Tandem LC Systems</h4><ul><li>Supported systems:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744">Vanquish Horizon</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/743">Vanquish Flex</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/397">Vanquish Core</a></li></ul></li><li>Sampler: Split sampler</li><li>Detector: One out of the complete detector portfolio and/or one mass spectrometer</li><li>Software control:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a></li><li>Standard System Integration for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758">Thermo Scientific™ Xcalibur™ software</a></li></ul></li></ul><h3>Vanquish Tandem LC systems</h3><div><div><div><div>Gradient separations are commonly used in LC or LC-MS. Column reconditioning, such as washing and re-equilibration, takes time and reduces your overall sample throughput. The Vanquish Tandem LC systems eliminate these limitations and improve productivity by:<ul><li>Increasing sample throughput without modifying a validated method</li><li>Improving return on investment by maximizing mass spectrometer utilization</li><li>Reducing column carry over through extended column washing without sacrificing throughput</li><li>Simplifying method setup with automatic method conversion from your original gradient method</li></ul></div></div></div></div><div>&nbsp;</div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Tandem_LC_Systems_4_a031ad3221.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Tandem_LC_Systems_4_a031ad3221.jpg 245w," sizes="100vw" width="1749" height="530"><h4>Throughput increase using Tandem LC</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Tandem_LC_Systems_3_6bc55942ed.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems: Throughput increase by Tandem LC is between 10–80% for most common conditions." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Tandem_LC_Systems_3_6bc55942ed.jpg 245w," sizes="100vw" width="894" height="434">The Vanquish Tandem LC system splits the analysis between two pumps and two columns. One pump delivers the analytical gradient while the second pump runs the reconditioning gradient. This configuration allows you to use two identical columns to run your sequence faster without compromising data quality.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Tandem_LC_Systems_5_d818007fe4.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems: Reproducible results for the Thermo Scientific™ Vanquish™ Horizon Tandem UHPLC system—Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap™ mass spectrometer showing an overlay of five total ion current chromatograms. The sample shown is digested Infliximab using the Thermo Scientific™ SMART ™ Digest Kit." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Tandem_LC_Systems_5_d818007fe4.jpg 245w," sizes="100vw" width="1123" height="499"><h4>Simply upgrade your method</h4>Implementing Tandem LC-MS into your workflow is easy with compliance-ready Chromeleon software for LC or LC-MS users and Thermo Scientific Standard Instrument Integration (SII) for Thermo Scientific Xcalibur software for LC-MS users.A simple dedicated instrument method wizard enables you to create new methods without the need for additional training.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Tandem_LC_Systems_0c7f310b1e.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Tandem_LC_Systems_0c7f310b1e.jpg 193w," sizes="100vw" width="353" height="285"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Tandem_LC_Systems_2_47128950b2.jpg" alt="Thermo Scientific Vanquish Tandem LC Systems: Chromeleon CDS and SII for Xcalibur guides users through the implementation of a Tandem LC or LC-MS method with automatic system volume considerations and gradient formation of the reconditioning pump." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Tandem_LC_Systems_2_47128950b2.jpg 160w," sizes="100vw" width="361" height="353"><h3>Thermo Scientific LC portfolio overview</h3><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_LC_portfolio_overview_2db5044366.jpg" alt="Thermo Scientific LC portfolio overview." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_LC_portfolio_overview_2db5044366.jpg 188w," sizes="100vw" width="795" height="660">

Thermo Scientific Vanquish Charged Aerosol Detectors

Thermo Scientific Vanquish CAD detectors deliver near-universal detection with high sensitivity, wide dynamic range, gradient compatibility, and compliance-ready design.

Expand your detection range and achieve accurate quantitation in High-Performance Liquid Chromatography (HPLC) with the near-universal Charged Aerosol Detector (CAD). Featuring a wide linear and dynamic range along with a uniform response independent of chemical structure, Thermo Scientific™ Vanquish™ CAD systems allow you to measure virtually all non-volatile and many semi-volatile analytes at low-nanogram to sub-nanogram concentrations. Confidently quantify both trace impurities and highly concentrated active compounds in a single analysis.<h3>Key Benefits</h3><ul><li>Standard-free quantitation of unknowns – uniform response enables reliable single-calibrant quantitation</li><li>Superior sensitivity – 10× lower LODs compared to ELSD, LOQs as low as 1–20 ng on-column</li><li>Wide dynamic range – linear up to 2 orders of magnitude, dynamic up to 4 orders</li><li>Compatible with gradient elution – maintain uniform response using inverse gradient, unlike RID</li><li>Integrated diverter valve – prevents contamination, directs unwanted matrix to waste, and improves quantitation of early-eluting compounds</li><li>Compliance and data integrity – full qualification of method parameters, audited flow path control, and seamless integration with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Thermo Scientific Chromeleon™ CDS</a></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Charged_Aerosol_Detectors_1_e135b2d6fe.jpg" alt="Thermo Scientific Vanquish Charged Aerosol Detectors" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Charged_Aerosol_Detectors_1_e135b2d6fe.jpg 209w," sizes="100vw" width="650" height="486"><h3>Technical Features</h3><ul><li>Detection mode: Records up to 4 unique power values for optimal linear response</li><li>Gas requirements: Nitrogen (recommended) or compressed air</li><li>Dynamic range: Up to 4 orders of magnitude</li><li>Data collection rate: Up to 200 Hz</li><li>Compatibility: Designed for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=37&amp;manufacturerItems=3&amp;search=Vanquish">Thermo Scientific™ Vanquish™ HPLC and UHPLC systems</a>; integration with other systems possible</li><li>Outputs: Digital and analog output options available</li><li>Safety features: Overpressure relief, leak sensing, high-temperature shutoff, flood prevention, and diagnostic monitoring</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Charged_Aerosol_Detectors_3_335506f860.jpg" alt="Thermo Scientific Vanquish Charged Aerosol Detectors" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Charged_Aerosol_Detectors_3_335506f860.jpg 245w," sizes="100vw" width="650" height="378"><h3>Product Portfolio</h3><ul><li>Vanquish CAD HP – broad temperature range (ambient, 10–100 °C), versatile high-performance configuration</li><li>Vanquish CAD FP – fixed-point operation at defined set temperatures (ambient, 35 °C, 50 °C, 70 °C)</li><li>Vanquish CAD H – heating-enabled design (ambient +5 to +100 °C)</li><li>Vanquish CAD F – fixed-temperature options (35 °C, 50 °C, 70 °C)</li><li>Vanquish CAD HP, Analog Output – full performance with analog signal output</li><li>Vanquish CAD HP, Digital Output – full performance with digital data output</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Vanquish_Charged_Aerosol_Detectors_2_795050403c.jpg" alt="Thermo Scientific Vanquish Charged Aerosol Detectors" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Vanquish_Charged_Aerosol_Detectors_2_795050403c.jpg 245w," sizes="100vw" width="650" height="397">

Waters Charged Aerosol Detector (CAD)

Waters Charged Aerosol Detector (CAD) enables universal quantitation of non-volatile and semi-volatile compounds independent of UV activity, with wide dynamic range and seamless integration with Empower™ Software.

<h3>Expand Your Detection Range with a Detector Purpose-Built for Empower™ Software</h3>Empower™ Software–based laboratories require detection solutions that are easy to adopt, robust, and capable of accurately quantifying non-volatile and semi-volatile compounds—regardless of chemical structure or UV activity. The Waters Charged Aerosol Detector (CAD) is purposefully engineered to meet these demands, delivering universal, reliable detection fully integrated with Waters chromatography systems and Empower™ or MassLynx™ Software.With a wide dynamic range exceeding four orders of magnitude, the Waters CAD enables simultaneous quantitation of low-level, non-UV-active analytes, along with related impurities and excipients, in a single run—helping laboratories reduce method development time, increase productivity, and improve process control.<h4>Key Benefits</h4><ul><li>Universal detection without UV limitations Detect and quantify non-volatile and semi-volatile compounds independent of chromophore presence.</li><li>Wide dynamic range (&gt;4 orders of magnitude) Confidently quantify trace-level components and major constituents simultaneously.</li><li>Purpose-built for Empower™ Software Seamless integration into regulated workflows with Empower-based qualification and long-term software compatibility.</li><li>Compatible across Waters LC platforms Integrates easily with Alliance™, Arc™, ACQUITY™ Arc, ACQUITY™ H-Class, ACQUITY™ I-Class, and UPLC™ Systems.</li><li>Designed for robustness and ease of use Optimized hardware, simplified maintenance, and enhanced system protection features minimize downtime.</li></ul><h4>Technical Specifications</h4><h5>Detector Performance</h5><ul><li>Detector flow rate: 0.010–2.000 mL/min</li><li>Dynamic range: Greater than four orders of magnitude (four individual power values)</li><li>Sampling rate: Up to 200 Hz</li><li>Number of channels: Four (4), supported by four (4) independent background current channels</li></ul><h5>Temperature Control</h5><ul><li>Evaporation temperature:<ul><li>Ambient (off)</li><li>Controlled range: 10 °C to 100 °C (0.1 °C increments)</li><li>Minimum of 5 °C above ambient</li></ul></li></ul><h5>Gas Requirements</h5><ul><li>Inert gas supply: Nitrogen (recommended) or compressed air</li><li>Inlet pressure: 4.83–5.52 bar (483–552 kPa, 70.0–80.0 psi)</li><li>Gas consumption: Typically ≤ 5 L/min</li></ul><h5>Diagnostics and Monitoring</h5><ul><li>Diagnostic channels include:<ul><li>Nebulizer gas flow and pressure (psi)</li><li>Evaporator temperature (°C)</li><li>Ion trap voltage (V)</li><li>Charger current (µA)</li><li>Mixing chamber temperature (°C)</li></ul></li></ul><h5>Electrical and Physical Specifications</h5><ul><li>Power requirements: 100–240 VAC</li><li>Dimensions:<ul><li>Width: 34.3 cm (13.5 in)</li><li>Height: 20.8 cm (8.2 in)</li><li>Depth: 61.0 cm (24.0 in)</li></ul></li><li>Biocompatible: No</li></ul><h5>Software and System Compatibility</h5><ul><li>Optimized for Empower™ Software and MassLynx™ Software control</li><li>Robust chromatography data system (CDS) communications to minimize downtime</li><li>Backward compatibility to Empower™ 3 Feature Release 4 Service Release 3 (SR3)</li><li>Fully compatible with systems qualification and validation requirements in regulated environments</li></ul><h5>Seamless Method Transfer Across Laboratories</h5>The Waters CAD is designed to simplify method transfer within organizations and across external laboratories. It maintains method simplicity while adding enhanced tools for equivalency and reproducibility, reducing the need for revalidation when transitioning from legacy or third-party CAD solutions.The intuitive ACQUITY™ console control panel enables straightforward operation and simplifies replacement of outdated technology across laboratory networks.<h5>Designed for Ease of Use and Reliability</h5><ul><li>Single filter manifold with push/pull filter replacement</li><li>Easy-to-replace nebulizer for improved serviceability</li><li>Intelligent divert valve automatically sends flow to waste if gas pressure is lost or system errors occur</li><li>Enhanced leak detection and flood protection, safeguarding instruments in the event of CDS communication loss</li></ul><h5>Recommended Use</h5>Accurate quantitation in:<ul><li>HPLC, UHPLC, and UPLC separations</li><li>Preparative and purification workflows</li><li>Pharmaceutical QC and R&amp;D</li><li>Analysis of excipients, lipids, sugars, surfactants, and non-UV-active impurities</li></ul>

Thermo Scientific Transcend Multichannel UHPLC Systems

Thermo Scientific Transcend Multichannel UHPLC Systems are advanced LC-MS systems with a multichannel UHPLC architecture that increase analytical productivity and maximize mass spectrometer utilization without compromising data quality.

Thermo Scientific™ Transcend™ Multichannel UHPLC Systems are advanced LC-MS platforms designed for laboratories that demand higher throughput, improved efficiency, and stronger return on investment. Their multichannel UHPLC architecture connects up to four fully independent LC channels to a single mass spectrometer, dramatically increasing system utilization without compromising data quality.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_8_5f824e5e08.jpg" alt="Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: The Transcend LX systems feature either 2 or 4 (shown) completely independent LC channels." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_8_5f824e5e08.jpg 245w," sizes="100vw" width="955" height="404"><h3>Key benefits</h3><ul><li>Significantly increased LC-MS throughput</li><li>Maximized mass spectrometer utilization</li><li>Maintained sensitivity, selectivity, and robustness</li><li>Reduced operating costs and analysis time</li><li>Scalable configurations tailored to laboratory needs</li></ul><h3>Multichannel UHPLC architecture</h3>Conventional single-channel LC-MS systems process samples sequentially, leaving the mass spectrometer idle during injection, column washing, and equilibration. Transcend systems overcome this limitation by intelligently interleaving injections from multiple LC channels, delivering data to the mass spectrometer only during relevant retention time windows and enabling substantial productivity gains.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_5_ab4093366d.jpg" alt="Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: Injections are interleaved to maximize throughput and mass spectrometer use." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_5_ab4093366d.jpg 169w," sizes="100vw" width="831" height="764"><h3>Vanquish UHPLC platform</h3>Thermo Scientific™ Transcend™ Multichannel UHPLC Systems are built on proven <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=40&amp;manufacturerItems=3&amp;search=vanquish">Thermo Scientific Vanquish UHPLC</a> technology. The Transcend multichannel architecture integrates up to four independent Vanquish-based UHPLC channels into a single LC-MS platform.The Vanquish UHPLC platform provides:<ul><li>high mechanical and operational robustness</li><li>reproducible long-term performance</li><li>precise gradient formation and stable flow control suitable for multichannel operation</li><li>flexible pump configurations (binary or quaternary) tailored to application requirements</li></ul>By combining the Transcend multichannel concept with Vanquish UHPLC technology, laboratories can achieve high throughput without compromising chromatographic performance, sensitivity, or system reliability.<h3>System configurations</h3><ul><li>Transcend LX and VLX Designed for high-throughput LC-MS workflows without on-line sample cleanup<ul><li>2- and 4-channel configurations</li><li>Fully independent LC channels</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_1567f69a93.jpg" alt="Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: Transcend LX UHPLC Systems" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_1567f69a93.jpg 245w," sizes="100vw" width="968" height="500"><ul><li>Transcend TLX Designed for workflows requiring automated on-line sample cleanup<ul><li>1-, 2-, and 4-channel configurations</li><li>Integrated TurboFlow™ technology</li></ul></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_2_8ac2e32016.jpg" alt="Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: Transcend TLX UHPLC Systems" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_2_8ac2e32016.jpg 245w," sizes="100vw" width="1674" height="523">Each LC channel operates independently, allowing identical methods to run in parallel or multiple methods to be combined within a single system.<h3>On-line sample cleanup with TurboFlow™ technology (TLX)</h3>TurboFlow™ technology enables rapid on-line removal of matrix interferences directly within the LC-MS system, resulting in:<ul><li>Reduced or eliminated manual sample preparation</li><li>Direct injection of complex biological and environmental samples</li><li>Improved sensitivity and reproducibility</li><li>Increased method robustness and simplified workflows</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_3_efccaa3928.jpg" alt="Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: On-line sample cleanup with TurboFlow™ technology (TLX)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_3_efccaa3928.jpg 245w," sizes="100vw" width="1105" height="556"><h3>System control and software</h3>All system functions are managed by Thermo Scientific™ Aria™ MX software, providing:<ul><li>Control of pumps, valves, gradients, and injection scheduling</li><li>Intuitive multichannel method setup</li><li>Parallel execution of up to four methods</li><li>A user-friendly graphical interface for complex LC-MS workflows</li></ul>Aria MX software is fully compatible with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758">Thermo Scientific™ Xcalibur™</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77">Thermo Scientific™ TraceFinder™</a> software.<h3>Mass spectrometer compatibility</h3>Transcend systems are designed as a flexible LC-MS platform compatible with a broad range of Thermo Fisher Scientific mass spectrometers, including:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=20&amp;manufacturerItems=3&amp;search=tsq">TSQ triple quadrupole systems</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3&amp;search=orbitrap">High-resolution Orbitrap instruments</a></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_4_fc2c09672b.jpg" alt="Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: Transcend TLX-2 system with a TSQ Altis Plus triple quadrupole mass spectrometer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_4_fc2c09672b.jpg 245w," sizes="100vw" width="1178" height="646"><h3>Typical application areas</h3><ul><li>Clinical research and bioanalysis</li><li>Pharmaceutical development and quality control</li><li>Toxicology</li><li>Environmental analysis</li><li>High-throughput screening workflows</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_6_db70763211.jpg" alt="Thermo Scientific™ Transcend™ Multichannel UHPLC Systems: Retention time (A) and peak area (B) reproducibility of a high-throughput targeted proteomics assay for quantifying SARS-CoV-2 peptides in clinical research samples across five days. The Transcend TLX-4 UHPLC system was used for on-line sample clean-up and analysis of 500 respiratory tract swab samples per day. (Cardozo, K.H.M., Lebkuchen, A., Okai, G.G. et al. Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts. Nat Commun 11, 6201 (2020))" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Transcend_Multichannel_UHPLC_Systems_6_db70763211.jpg 245w," sizes="100vw" width="1489" height="862">

KNAUER AZURA HTQC UHPLC System

KNAUER AZURA HTQC UHPLC (1240 bar) enables high-throughput QC with parallel analysis and column re-equilibration for faster workflows.

The KNAUER AZURA HTQC UHPLC (1240 bar) is a high-performance UHPLC system designed for high-throughput quality control (QC) where speed, robustness, and reproducibility are essential.The system features a unique concept of parallel analysis and offline column re-equilibration, significantly increasing productivity. With two gradient pumps, one column performs the analytical run while the second is re-equilibrated in backflush mode.This architecture enables:<ul><li>≥30% reduction in cycle time</li><li>increased sample throughput</li><li>column protection from matrix contamination</li><li>consistent retention times</li></ul>It is ideal for pharmaceutical QC laboratories requiring automation and validated workflows.<h4>Key Features</h4><ul><li>UHPLC system up to 1240 bar (124 MPa)</li><li>Dual gradient pumps (HPG + LPG)</li><li>Parallel analysis and column re-equilibration</li><li>Backflush mode for column protection</li><li>≥30% faster analysis cycles</li><li>High flow reproducibility</li><li>Autosampler with cooling</li><li>Column thermostat (Peltier)</li><li>Fully automated modular system</li><li>MS detector compatibility</li></ul><h4>System Configuration</h4>Included components:<ul><li>Eluent tray E 2.1L</li><li>Pump P 8.1L (HPG)</li><li>Pump P 6.1L (LPG)</li><li>Liquid handler LH 8.1 + Robotic Cooler</li><li>Column thermostat CT 2.1</li><li>Column switching &amp; backflush valve</li><li>Accessories and tools</li></ul><h3>Technical Specifications</h3><h4>Pumps</h4><h5>P 8.1L (HPG – analytical pump):</h5><ul><li>Max pressure: 1240 bar</li><li>Flow rate: up to 5 mL/min</li><li>Adaptive pulsation compensation</li><li>Degasser + binary solvent selection</li><li>Low baseline noise</li></ul><h5>P 6.1L (LPG – re-equilibration):</h5><ul><li>Quaternary gradient</li><li>Integrated degasser</li><li>Low dwell volume</li><li>Inline filter</li></ul><h4>Autosampler</h4><h5>LH 8.1:</h5><ul><li>XYZ autosampler</li><li>Capacity: up to 390 samples</li><li>Temperature: 4–40 °C</li><li>Low carryover</li></ul><h4>Column Thermostat</h4><h5>CT 2.1:</h5><ul><li>Peltier controlled</li><li>Above and below ambient operation</li><li>High precision</li></ul><h4>Valve System</h4><ul><li>Column switching + backflush</li><li>Up to 1200 bar</li><li>Parallel column operation</li></ul><h4>Detection</h4><ul><li>Compatible with all HPLC detectors</li><li>Ideal for LC-MS coupling</li></ul><h4>HTQC Concept</h4><ul><li>Independent flow paths (analysis vs re-equilibration)</li><li>Backflush re-equilibration</li><li>Parallel operation</li><li>Increased throughput without compromise</li></ul><h4>Typical Applications</h4><ul><li>Pharmaceutical QC</li><li>Routine validated workflows</li><li>High sample throughput environments</li><li>Reversed-phase HPLC</li></ul>

KNAUER High Precision AZURA P 6.1L HPLC Pump

KNAUER AZURA P 6.1L HPLC pump with up to 1000 bar, precise gradient control, and low pulsation for reliable UHPLC workflows.

The KNAUER AZURA P 6.1L is a high-precision analytical HPLC pump designed for accurate and reproducible solvent delivery in both HPLC and UHPLC applications.The pump combines essential features of a modern pumping system, including low pulsation, high pressure capability, precise gradient formation, and flexible configuration. It is available in both HPG (high-pressure gradient) and LPG (low-pressure gradient) versions, with a flow range up to 50 mL/min.The system uses a dual serial piston mechanism with active pulsation compensation, ensuring excellent flow stability under all conditions. A low-dead-volume microfluidic mixer enables efficient and precise solvent mixing.Different pump heads are available:<ul><li>up to 10 mL/min at 862 bar</li><li>up to 5 mL/min at 1000 bar</li></ul>Optional configurations include biocompatible materials (PEEK, ceramic, titanium) and specialized setups for normal phase applications (e.g., hexane, heptane).<h4>Key Features</h4><ul><li>High-precision analytical HPLC/UHPLC pump</li><li>Pressure up to 1000 bar</li><li>Flow rates up to 50 mL/min</li><li>Dual piston system with low pulsation</li><li>Active pulsation compensation</li><li>HPG and LPG gradient configurations</li><li>Microfluidic mixer (50–600 µl)</li><li>RFID pump head detection</li><li>Optional degasser</li><li>Biocompatible configurations available</li><li>Robust operation with demanding solvents</li></ul><h3>Technical Specifications</h3><h4>Solvent Delivery</h4><ul><li>Pump type: analytical HPLC pump</li><li>Mechanism: dual serial piston</li><li>Flow range: 0.001 – 50 mL/min</li><li>Max pressure: up to 1000 bar</li><li>Flow accuracy: ± 0.25%</li><li>Precision: ≤ 0.04% RSD</li></ul><h4>Gradient</h4><ul><li>Range: 0–100% (0.1% steps)</li><li>HPG accuracy: ± 0.3%</li><li>HPG precision: &lt; 0.1% RSD</li><li>LPG accuracy: ± 0.3% to ± 2%</li><li>LPG precision: &lt; 0.5% RSD</li></ul><h4>Mixing</h4><ul><li>Microfluidic mixer</li><li>Volumes: 50 / 100 / 200 / 400 / 600 µl</li><li>Low dead volume</li></ul><h4>Degasser (optional)</h4><ul><li>2–4 channels</li><li>Max flow: 10 mL/min per channel</li><li>Residual O₂: &lt; 0.5 ppm</li><li>Membrane: fluoropolymer</li></ul><h4>Materials</h4><ul><li>PEEK, Tefzel®, Systec AF</li><li>Optional biocompatible materials (titanium, ceramic)</li></ul><h4>Control &amp; Communication</h4><ul><li>LAN, analog inputs</li><li>Event outputs (TTL, relay)</li><li>Optional mobile control</li><li>RFID pump head detection</li></ul><h4>Operating Conditions</h4><ul><li>Temperature: 4–40 °C</li><li>Humidity: &lt; 90% non-condensing</li></ul><h4>Dimensions &amp; Power</h4><ul><li>Dimensions: 361 × 208 × 523 mm</li><li>Weight: 14.1 kg</li><li>Power supply: 100–240 V, 50–60 Hz</li><li>Power consumption: max 100 W</li></ul>

KNAUER High Precision AZURA P 8.1L UHPLC Pump

KNAUER AZURA P 8.1L UHPLC pump with up to 1240 bar, adaptive pulsation compensation, and high precision for demanding UHPLC applications.

The KNAUER AZURA P 8.1L is a high-performance UHPLC pump designed for ultra-fast and high-resolution chromatographic applications. With a maximum pressure of 1240 bar (124 MPa) and flow rates up to 5 mL/min, it meets the demands of modern UHPLC workflows.The pump features a dual serial piston mechanism combined with adaptive pulsation compensation, which operates independently of flow rate, pressure, and solvent type through real-time monitoring of eluent compressibility and variable piston stroke.Together with KNAUER’s advanced piston drive technology, this results in exceptional flow reproducibility under all operating conditions. High-precision in-house manufacturing ensures long piston seal lifetime and maximum system uptime.<h4>Key Features</h4><ul><li>UHPLC pump up to 1240 bar</li><li>Flow rates up to 5 mL/min</li><li>Dual serial piston system</li><li>Adaptive pulsation compensation</li><li>Excellent flow reproducibility</li><li>HPG gradient capability</li><li>Integrated 4-channel degasser</li><li>RFID pump head detection</li><li>Low baseline noise</li><li>High durability and long lifetime</li><li>Compatible with UHPLC and LC-MS systems</li></ul><h3>Technical Specifications</h3><h4>Solvent Delivery</h4><ul><li>Pump type: analytical UHPLC pump</li><li>Mechanism: dual serial piston</li><li>Max pressure: 1240 bar</li><li>Flow rate: up to 5 mL/min</li><li>Flow accuracy: ± 0.25%</li><li>Precision: ≤ 0.04% RSD</li></ul><h4>Gradient</h4><ul><li>Type: HPG (high-pressure gradient)</li><li>Range: 0–100% (0.1% steps)</li><li>Accuracy: ± 0.3%</li><li>Precision: &lt; 0.1% RSD</li></ul><h4>Degasser</h4><ul><li>Channels: 4</li><li>Max flow: 5 mL/min per channel</li><li>Residual O₂: &lt; 0.5 ppm</li><li>Technology: fluoropolymer membrane</li></ul><h4>Connections</h4><ul><li>Inlet: UNF 1/4-28 (1/8” tubing)</li><li>Outlet: UNF 10-32 (1/16” capillary)</li></ul><h4>Materials</h4><ul><li>PEEK, Tefzel®, Systec AF</li><li>Compatible with most solvents (except HCl and halogenated hydrocarbons)</li></ul><h4>Control &amp; Communication</h4><ul><li>LAN, analog inputs</li><li>Event outputs (TTL, relay)</li><li>Optional mobile control</li><li>RFID pump head detection</li></ul><h4>Safety &amp; Operating Conditions</h4><ul><li>Leak sensor</li><li>Soft start and programmable pressure limits</li><li>Temperature: 4–40 °C</li><li>Humidity: &lt; 90% non-condensing</li></ul><h4>Dimensions &amp; Power</h4><ul><li>Dimensions: 361 × 208 × 523 mm</li><li>Weight: 26.7 kg</li><li>Power supply: 100–240 V, 50–60 Hz</li><li>Power consumption: max 310 W</li></ul>

KNAUER AZURA Autosampler AS 6.1L

KNAUER AZURA AS 6.1L autosampler with up to 768 samples, ultra-low carryover, and fast injection cycles for HPLC/UHPLC workflows.

The KNAUER AZURA® AS 6.1L is a high-performance autosampler designed for automated sample injection in HPLC/UHPLC systems, offering high capacity, precision, and flexibility for both routine and high-throughput applications.The autosampler supports:<ul><li>up to 768 sample positions (microtiter plates)</li><li>up to 108 standard 1.5 mL vials</li></ul>A highly effective internal and external needle wash system minimizes carryover to extremely low levels. The system is also fast, with a complete injection cycle taking less than one minute, including needle washing.Three injection modes are supported:<ul><li>full loop filling (highest precision)</li><li>partial loop filling (variable volumes)</li><li>µl pickup (loss-free injection of small volumes)</li></ul>The integrated ILD™ (Intermediate Loop Decompression) valve ensures accurate high-pressure injections without sample dilution and reduces pressure spikes, protecting the column.<h4>Key Features</h4><ul><li>Autosampler for HPLC/UHPLC systems</li><li>Up to 768 sample positions</li><li>Up to 108 vials (1.5 mL)</li><li>Ultra-low carryover (&lt; 0.0003%)</li><li>Fast injection cycle (&lt; 1 min)</li><li>3 injection modes (full loop, partial loop, µl pickup)</li><li>ILD™ technology for high-pressure injection</li><li>PASA™ (pressure-assisted sample aspiration)</li><li>Optional sample cooling (4–40 °C)</li><li>Missing vial/plate detection</li><li>Supports precolumn derivatization</li></ul><h3>Technical Specifications</h3><h4>Sample Injection</h4><ul><li>Injection volume range: 0.1 µl – 10 mL</li><li>Precision:<ul><li>Full loop: &lt; 0.3% RSD</li><li>Partial loop: &lt; 0.5% RSD</li><li>µl pickup: &lt; 1.0% RSD</li></ul></li><li>Accuracy: 0.2 µl (250 µl syringe)</li></ul><h4>Performance</h4><ul><li>Carryover:<ul><li>&lt; 0.0015% (standard)</li><li>&lt; 0.0003% (extended wash)</li></ul></li><li>Injections per vial: max. 9</li><li>Cycle time:<ul><li>7 s (same vial)</li><li>14 s (different vials)</li><li>&lt; 60 s total</li></ul></li></ul><h4>Capacity &amp; Compatibility</h4><ul><li>Microtiter plates: ANSI SLAS standard</li><li>Max vial/plate height: 47 mm</li></ul><h4>Additional Features</h4><ul><li>Headspace pressure (bubble prevention)</li><li>Precolumn derivatization</li><li>Sample tray cooling (optional, 4–40 °C)</li><li>Vial detection sensor</li></ul><h4>Materials</h4><ul><li>ETFE, PTFE, stainless steel, glass, Vespel</li><li>Wide chemical compatibility</li></ul><h4>Control &amp; Communication</h4><ul><li>LAN / Ethernet</li><li>Analog inputs</li><li>TTL inputs (start, stop, freeze)</li><li>Relay output (alarm, marker)</li></ul><h4>Operating Conditions</h4><ul><li>Temperature: 10–40 °C</li><li>Humidity: 20–80%</li></ul>

KNAUER AZURA CT 2.1 Column Thermostat

KNAUER AZURA CT 2.1 column thermostat with Peltier control (5–85 °C), high stability, and optional eluent pre-heating for precise HPLC separations.

The KNAUER AZURA CT 2.1 is a cost-effective column thermostat designed for precise and stable temperature control in HPLC applications.The instrument uses a microprocessor-controlled Peltier element, enabling both heating and cooling in the range of 5–85 °C. Its high temperature stability allows for programming linear and non-linear temperature gradients, improving separation performance.For advanced applications, the system can be optionally equipped with an eluent pre-heating cartridge, ensuring consistent temperature conditions, improved selectivity, and better peak shapes.Its compact design supports flexible integration and accommodates multiple columns.<h4>Key Features</h4><ul><li>Column thermostat for HPLC applications</li><li>Temperature range 5–85 °C</li><li>Peltier technology (heating and cooling)</li><li>High temperature stability (±0.1 °C)</li><li>Accurate temperature control (±0.2 °C)</li><li>Programmable temperature gradients</li><li>Optional eluent pre-heating</li><li>Supports multiple columns</li><li>Compact and robust design</li><li>Built-in safety features</li></ul><h3>Technical Specifications</h3><h4>Thermostatting</h4><ul><li>Technology: Peltier (heating &amp; cooling)</li><li>Temperature range: 5–85 °C</li><li>Heating/cooling rate: 2 °C/min</li><li>Accuracy: ± 0.2 °C</li><li>Stability: ± 0.1 °C</li><li>Air circulation: fan-assisted bidirectional flow</li></ul><h4>Column Compartment</h4><ul><li>Max number of columns: up to 8</li><li>Max length: 325 mm</li><li>Max diameter: 35 mm</li><li>Compatibility:<ul><li>125 mm × 4.6 mm ID (with precolumn)</li><li>300 mm × 4.6 mm ID</li><li>300 mm × 16 mm ID</li></ul></li></ul><h4>Internal Dimensions</h4><ul><li>90 × 390 × 47 mm</li></ul><h4>Safety</h4><ul><li>Self-check and auto-calibration at startup</li><li>Adjustable shut-off temperature</li><li>Leak sensor with acoustic alarm</li></ul><h4>Control &amp; Communication</h4><ul><li>LAN interface</li><li>Optional Mobile Control (stand-alone operation)</li></ul><h4>Power &amp; Dimensions</h4><ul><li>Power supply: 90–230 V, 50–60 Hz</li><li>Power consumption: 100 W</li><li>Dimensions: 150 × 470 × 310 mm</li><li>Weight: 8.4 kg</li></ul><h4>Optional Accessories</h4><ul><li>Eluent pre-heating cartridge<ul><li>for capillaries 0.1 mm / 0.18 mm ID</li></ul></li></ul>

KNAUER AZURA Valve Unifier VU 4.1

KNAUER AZURA VU 4.1 valve drive enables fast automated valve switching with RFID identification for HPLC/UHPLC systems.

The KNAUER AZURA VU 4.1 Valve Unifier is a compact valve drive unit designed for automated valve switching in HPLC/UHPLC systems. It enables precise and fast control of liquid flow paths, which is essential for applications such as column switching, sample injection, eluent selection, and fractionation.Due to its ultra-fast switching speed, the flow path is interrupted only briefly, significantly reducing pressure spikes and improving system stability.The device uses RFID technology for automatic valve identification, ensuring easy and error-free valve exchange. Integrated GLP data monitoring simplifies maintenance tasks such as rotor seal replacement.The unit can be operated:<ul><li>stand-alone via display</li><li>via software</li><li>through LAN or analog I/O</li></ul>This makes it easy to integrate into almost any LC system.<h4>Key Features</h4><ul><li>Universal valve drive for &gt;40 valve types</li><li>Ultra-fast switching (minimal pressure spikes)</li><li>RFID valve identification</li><li>GLP data monitoring for maintenance</li><li>Standalone operation via display</li><li>Integration into LC systems (LAN, analog, software)</li><li>Compact design</li><li>Easy maintenance and valve exchange</li><li>Flexible use in LC and industrial applications</li></ul><h3>Technical Specifications</h3><h4>Communication &amp; Control</h4><ul><li>Interfaces: LAN, display, terminal strip</li><li>Control:<ul><li>display (stand-alone)</li><li>software</li><li>event control</li></ul></li><li>Inputs:<ul><li>Home</li><li>Backward/Inject</li><li>Forward/Load</li><li>Start</li></ul></li><li>Outputs:<ul><li>Trigger out</li><li>Event output</li></ul></li></ul><h4>Performance &amp; Features</h4><ul><li>Ultra-fast valve switching</li><li>Reduced pressure peaks</li><li>Supports &gt;40 valve types</li><li>RFID valve recognition</li><li>GLP maintenance tracking</li></ul><h4>Operating Conditions</h4><ul><li>Temperature: 4–40 °C</li><li>Humidity: &lt; 90% non-condensing</li></ul><h4>Power &amp; Dimensions</h4><ul><li>Power supply: external 24 V DC</li><li>Power consumption: 65 W</li><li>Dimensions: 80 × 123 × 192 mm</li><li>Weight: 1.9 kg</li></ul><h4>Typical Applications</h4><ul><li>Column switching</li><li>Eluent selection</li><li>Sample injection</li><li>Fraction collection</li><li>LC workflow automation</li><li>Dosing systems in chemical industry</li></ul>

Products focused on HPLC - page 2

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