<p><strong>🎤Brad Haubrich </strong>(Touro University Nevada )</p><p><strong>Neglected and opportunistic infectious diseases affect some of the world’s most vulnerable populations—but often receive the least attention from traditional drug discovery pipelines.</strong></p><p>In this episode of Concentrating on Chromatography, host David Oliva sits down with Brad Haubrich to explore how early-stage drug discovery is being applied to fungal and parasitic pathogens, including those responsible for neglected tropical diseases and infections that disproportionately affect immunocompromised patients.</p><p>Brad shares how his lab approaches drug discovery when the pathogen is eukaryotic—and therefore biologically similar to humans—making selectivity one of the biggest challenges. The conversation covers:</p><ul><li>What defines neglected and opportunistic diseases—and why commercial incentives often fall short</li><li>Target-based vs. phenotypic drug discovery and when each approach makes sense</li><li>Using binding kinetics and residence time to improve selectivity and reduce off-target effects</li><li>Where chromatography, metabolomics, and mass spectrometry still play a critical role—even when not front-and-center</li><li>The growing (and realistic) role of AI in drug discovery, especially for underfunded disease areas</li><li>Why World Neglected Tropical Diseases Day (January 30) matters for raising awareness and accelerating progress</li></ul><p>This episode highlights how analytical chemistry, separation science, and biological insight intersect in the earliest stages of drug discovery—and why progress in this space depends as much on collaboration and curiosity as it does on technology.</p><h3><strong>Video Transcription</strong></h3><p>In this interview, Dr. Brad Haubrich discusses his research focus on neglected tropical diseases (NTDs) and opportunistic infections. His interest in this field originated during graduate and postdoctoral studies, particularly through work on African sleeping sickness. Neglected diseases are defined as conditions that predominantly affect populations in low- and middle-income countries, where both healthcare infrastructure and commercial incentives for drug development are limited. Similarly, opportunistic infections disproportionately impact immunocompromised individuals, including patients with cancer, transplant recipients, and those undergoing immunosuppressive therapy.</p><p>A key challenge in this area is the limited commercial interest in drug discovery, resulting in fewer therapeutic options despite significant unmet medical needs. The research emphasizes fungal and parasitic pathogens, which present distinct challenges compared to bacterial targets, particularly due to shared biological pathways between pathogens and human hosts.</p><h4><span><strong>Drug Discovery Strategies and Challenges</strong></span></h4><p>The discussion highlights two complementary approaches used in the lab: target-based and phenotypic drug discovery. Target-based strategies involve identifying and biochemically characterizing specific pathogen proteins, while phenotypic approaches assess compound activity directly in living cells. Each approach has advantages and limitations, particularly in identifying molecular targets and ensuring biological relevance.</p><p>A major scientific challenge is achieving selectivity. Because eukaryotic pathogens share many proteins with human cells, identifying compounds that selectively inhibit pathogen targets without affecting host systems is difficult. To address this, the lab incorporates <strong>binding kinetics</strong> as an early screening parameter. Instead of relying solely on traditional potency metrics, this approach evaluates how long a compound interacts with its target, enabling the identification of molecules with prolonged, selective binding (pseudo-irreversible inhibition), which may improve therapeutic specificity.</p><h4><span><strong>Role of Analytical Techniques and Chromatography</strong></span></h4><p>Although chromatography is not central to all workflows in the lab, it remains critical in several areas. It is widely used by collaborators for compound purification and plays an important role in emerging projects focused on <strong>pharmacognosy</strong>, where bioactive compounds are isolated from natural sources such as desert flora.</p><p>Chromatography, coupled with mass spectrometry, is also essential for <strong>metabolomics-based target identification</strong>. In phenotypic screening, where the molecular target is initially unknown, changes in metabolite profiles can provide insight into biochemical pathways affected by candidate compounds, supporting target deconvolution.</p><h4><span><strong>Impact, Training, and Future Directions</strong></span></h4><p>The success of research in neglected diseases is not solely measured by commercial outcomes but also by contributions to scientific knowledge and global health. Even incremental findings can support future therapeutic breakthroughs.</p><p>The interview also emphasizes the role of students, particularly in academic medical settings, where participation in early-stage drug discovery provides valuable insight into the full pipeline from molecular discovery to clinical application.</p><p>Looking ahead, <strong>artificial intelligence (AI)</strong> is identified as a key driver of innovation in drug discovery. AI tools can accelerate compound screening, predict bioactivity, and even suggest synthetic pathways. However, their effectiveness in neglected disease research is currently limited by the availability of large, high-quality datasets.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Drug Discovery Where the Market Doesn’t Go: Targeting Neglected & Opportunistic Pathogens

<p><strong>This study investigates the optimization and functional properties of kombucha enriched with </strong><i><strong>Prunus spinosa</strong></i><strong> juice and bee products (propolis, pollen, and bee bread). Advanced analytical techniques (HPLC–DAD, LC–MS/MS) revealed enhanced phenolic, amino acid, and antioxidant profiles, with bee bread enrichment showing the highest overall bioactive content and sensory acceptance.</strong></p><p>Propolis-enriched kombucha exhibited the strongest in vitro antidiabetic activity, while pollen enrichment provided the highest amino acid levels. Despite partial losses during digestion, significant retention of bioactives suggests potential postdigestive benefits. These findings highlight the potential of enriched kombucha as a functional beverage with nutraceutical value.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00008"><strong>From Chemistry to Functionality: HPLC–DAD/LC–MS/MS Characterization of Bee Product-Enriched Prunus spinosa L. Kombucha with In Vitro Antidiabetic Activity and Bioaccessibility</strong></a></h4><p>Melikenur Türkol, Çiğdem Yıldırım Maviş, and Seydi Yıkmış*</p><p>ACS Omega 2026, 11, 8, 14048–14063</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.6c00008">https://doi.org/10.1021/acsomega.6c00008</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>Kombucha tea, a traditionally fermented beverage, is believed to have originated as early as 200–300 BC. In recent decades, it has attracted growing global interest due to its recognized health-promoting properties and its deep cultural roots. <a href="javascript:void(0);">(1,2)</a> Sweetened tea derived from <i>Camellia sinensis</i> is transformed into kombucha through fermentation carried out by a SCOBY (Symbiotic Culture of Bacteria and Yeast), a mixed culture of yeasts and bacteria that forms a cellulose-rich biofilm throughout the process. <a href="javascript:void(0);">(3)</a> SCOBY represents a symbiotic consortium of microorganisms that comprises various yeast genera, including <i>Saccharomyces</i>, <i>Schizosaccharomyces</i>, <i>Zygosaccharomyces</i>, <i>Brettanomyces/Dekkera</i>, and <i>Pichia</i>, as well as both acetic and lactic acid bacteria. <a href="javascript:void(0);">(2)</a> During fermentation, the sugars formed contribute a mild sweetness to the tea, whereas the organic acids synthesized by acetic acid bacteria are responsible for its distinctive sour flavor. <a href="javascript:void(0);">(4)</a> Kombucha tea is traditionally brewed using black tea; however, alternative versions can also be produced with other types of tea. <a href="javascript:void(0);">(5)</a> It has been reported that herbal infusions and fruit juices can also be used for fermentation with the kombucha culture, and due to their chemical composition, these substrates have been shown to serve as promising alternatives. <a href="javascript:void(0);">(6)</a> The antioxidant potential of kombucha tea is primarily linked to its abundant phenolics, with flavonoids playing a significant contributory role. <a href="javascript:void(0);">(7)</a> A high anthocyanin content tends to enhance the antioxidant capacity. <a href="javascript:void(0);">(8)</a> The antioxidant activity of kombucha tea can be improved by incorporating anthocyanin-rich fruits. Accordingly, the use of wild fruits with high anthocyanin content─such as <i>Prunus spinosa</i> L. (blackthorn), <i>Prunus laurocerasus</i> (cherry laurel), and <i>Rubus idaeus</i> (red raspberry)─has been considered a practical way to improve the beverage’s nutritional, functional and sensorial properties. <a href="javascript:void(0);">(9)</a></p><p>Propolis is a naturally occurring material that honeybees gather from the resins of different trees and the buds of various plants. <a href="javascript:void(0);">(28,29)</a> Propolis is a chemically complex matrix comprising more than 300 identified components, including flavonoids, phenolic acids, and their ester derivatives, and is associated with a wide range of biological activities such as antiviral, antioxidant, antimicrobial, anti-inflammatory, antitumor, hepatoprotective, and immunomodulatory effects. <a href="javascript:void(0);">(30−34)</a> Minerals and vitamins are also present in high concentrations in propolis. <a href="javascript:void(0);">(28)</a> Bee pollen is formed when bees agglutinate pollen grains using their saliva, nectar, or honey and store them in granular form in their honeycomb cells. The key role of bee pollen is to ensure the colony’s survival by providing the necessary nutrients, thereby supporting development and maintenance processes. <a href="javascript:void(0);">(35)</a> Bee pollen is a natural product rich in bioactive compounds. More than 250 bioactive compounds have been identified in bee pollen derived from different plant species. These basic chemical components include lipids, amino acids, proteins, carbohydrates, fatty acids, phenolic compounds, carotenoids, flavonoids, vitamins, and bioelements. <a href="javascript:void(0);">(36)</a> Exhibits antihyperglycemic, anti-inflammatory, hepatoprotective, nephroprotective, and anticancer properties thanks to its rich and versatile component mixture. <a href="javascript:void(0);">(37,38)</a> Many of these have demonstrated antioxidant, antimicrobial, antidiabetic. They have shown promising results in the treatment of diabetes and obesity, and their potential as natural therapeutic agents has been evaluated. They have been found to increase insulin sensitivity, reduce oxidative stress, regulate appetite, adjust obesity-related hormone levels, and strengthen antioxidant defense systems. <a href="javascript:void(0);">(39,40)</a> The antimicrobial effect of bee bread has been extensively studied. Bakour et al. found that different bacterial species and fungi are sensitive to the hydromethanolic bee bread extract. <a href="javascript:void(0);">(41)</a> A vital bee byproduct, bee bread is formed after the pollen gathered by bees is enriched with nectar and bee saliva, leading to an ensuing lactic acid fermentation process inside the hives. <a href="javascript:void(0);">(42)</a> Bee bread is high in nutrition, with significant carbohydrates, proteins and lipids. It is a rich source of vitamins, phenolic compounds, minerals and essential amino acids, and exhibits multiple chemical activities. <a href="javascript:void(0);">(43,44)</a></p><p>The Response Surface Method (RSM) is one of the statistical optimization approaches. This method uses empirical modeling techniques to identify relationships between independent variables and the system response using experimental data. It also allows for determining optimal conditions for achieving the desired outcome by evaluating the effects of process variables on the system response. <a href="javascript:void(0);">(45−47)</a></p><p>Previous studies have not extensively investigated the sensory parameters (color, taste, smell, and general acceptance) of kombucha tea enriched with <i>P. spinosa</i> L. and bee products using RSM. The primary aim of this study was to optimize the sensory properties of kombucha formulations enriched with <i>P. spinosa</i> L. and bee-derived ingredients (bee bread, propolis, and pollen) using RSM to determine the most acceptable formulation. While many studies have examined kombucha enriched with various fruit and plant infusions, none have specifically investigated the impact of <i>P. spinosa</i> L. and bee products on kombucha’s sensory and bioactive properties. The study also evaluated the total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (DPPH•), total anthocyanin content (TAC), phenolic profile (HPLC), microbiological quality, and levels of organic acids amino acids and in vitro bioaccessibility in the developed samples, as well as their antidiabetic activity. RSM-based optimization offers a new methodological approach. It is expected to yield valuable data. This data will improve the functional and disease-fighting properties of kombucha.</p><h4><strong>2. Materials and Methods</strong></h4><h5><strong>2.9. Analysis of Phenolic Compounds (HPLC)</strong></h5><p>Chromatographic separation was carried out on an ACE Genix C18 column (250 × 4.6 mm, 5 μm; Agilent), following the procedure reported by Portu et al. (2017). <a href="javascript:void(0);">(56)</a> Phenolics were characterized using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1260+LC"><strong>Agilent 1260 HPLC</strong></a>–DAD system operated under gradient conditions at 30 °C with a flow rate of 0.80 mL/min. The mobile phase comprised solvent A (water supplemented with 0.1% phosphoric acid) and solvent B, applied according to a predefined gradient program. Samples were injected at a volume of 10 μL, and chromatograms were recorded at 280, 320, and 360 nm. Quantitative results were reported as μg/mL.</p><h5><strong>2.10. Analysis of Organic Acids (HPLC)</strong></h5><p>HPLC was used to determine organic acid profiles, following a method adapted with minor modifications from Coelho et al. <a href="javascript:void(0);">(57)</a> Organic acids were analyzed using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1260+LC"><strong>Agilent 1260 Infinity LC HPLC system</strong></a> fitted with refractive index (RID) and diode array (DAD) detectors. Samples were filtered through a 0.45 μm syringe filter, and a 20 μL aliquot was injected into an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1260+LC"><strong>Agilent Hi-Plex H ion-exchange column</strong></a> (300 × 7.7 mm). Chromatographic separation was carried out using 10.0 mM H<sub>2</sub>SO<sub>4</sub> as the mobile phase at a flow rate of 0.6 mL/min, with the column temperature set to 65 °C and the RID cell maintained at 35 °C. The total run time was 20 min. DAD additionally monitored tartaric, pyruvic, and acetic acids at 210 nm. Quantitative determination was carried out using external calibration standards, and the results were reported in g/L.</p><h5><strong>2.11. Determination of Free Amino Acids (LC–MS)</strong></h5><p>Amino acid composition was determined using a method adapted with minor modifications from the procedure described by Bilgin et al. (2019). <a href="javascript:void(0);">(58)</a> A gradient elution scheme involving mobile phases A and B was applied to obtain chromatographic separation at a flow rate of 0.7 mL/min, yielding a total run duration of 7.5 min. Tandem mass spectrometric analyses were performed on an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10"><strong>Agilent 6460 triple quadrupole LC–MS instrument</strong></a> (Agilent Technologies, Waldbronn, Germany) fitted with an electrospray ionization (ESI) interface. The mass spectrometer was operated with a gas temperature of 150 °C, a gas flow rate of 10 L/min, a nebulizer pressure of 40 psi, and a capillary voltage of +2000 V. All analyses were carried out in triplicate, and the results were reported as mg/100 mL.</p><h4><strong>3. Results and Discussion</strong></h4><h5><strong>3.4. Phenolic Compounds (HPLC)</strong></h5><p>As presented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00008#tbl1">Table 1</a>, the total quantified phenolic content differed significantly among the treatments (<i>p</i> &lt; 0.05). The control sample (BKT) exhibited the lowest phenolic concentration (106.35 ± 2.25 μg/mL), whereas pollen supplementation (PL-BKT) resulted in a substantial increase, reaching 159.23 ± 4.82 μg/mL. Further enhancement of the phenolic pool was achieved through propolis (PB-BKT: 178.66 ± 5.04 μg/mL) and bee bread (BB-BKT: 186.15 ± 0.97 μg/mL) supplementation. No statistically significant difference was observed between PB-BKT and BB-BKT in total quantified phenolics; however, both treatments exhibited significantly higher values than PL-BKT and the control, indicating a more pronounced phenolic enrichment.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_8_14048_14063_Table_1_Changes_in_Total_Phenolic_Compounds_of_Kombucha_Samples_Subjected_to_BKT_PB_BKT_PL_BKT_and_BB_BKT_5a50c7851f.jpg" alt="ACS Omega 2026, 11, 8, 14048–14063: Table 1. Changes in Total Phenolic Compounds of Kombucha Samples Subjected to BKT, PB-BKT, PL-BKT and BB-BKT. Data are presented as mean ± standard deviation (n = 3). Values denoted by different letters within the same row indicate statistically significant differences (p &lt; 0.05). BKT: Blackthorn kombucha tea; PB-BKT: Propolis blackthorn kombucha tea; PL-BKT: Pollen blackthorn kombucha tea; BB-BKT: Bee bread blackthorn kombucha tea. n.d.: could not be detected." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_8_14048_14063_Table_1_Changes_in_Total_Phenolic_Compounds_of_Kombucha_Samples_Subjected_to_BKT_PB_BKT_PL_BKT_and_BB_BKT_5a50c7851f.jpg 159w," sizes="100vw" width="1054" height="1034"></p><p>The compound-level distribution suggests that the observed elevation in total phenolics is driven by selective, matrix-dependent enrichment. Bee bread is a functional food thanks to its bioactive compounds and chemical diversity. <a href="javascript:void(0);">(72)</a> The marked increase in chlorogenic acid concentration in BB-BKT, to 79.04 ± 4.66 μg/mL, was a key contributor to the elevated total phenolic content in this group. In contrast, chlorogenic acid remained undetectable or present at negligible levels in the other treatments. Likewise, the enrichment of thermosonicated kiwi juice with bee bread resulted in a statistically significant elevation in chlorogenic acid levels. <a href="javascript:void(0);">(68)</a> The highest catechin hydrate concentration was observed in PL-BKT (121.2 ± 3.36 μg/mL), indicating that pollen supplementation preferentially enhances the flavan-3-ol fraction. In contrast, the markedly elevated levels of quercetin (47.32 ± 2.07 μg/mL) and naringenin (17.59 ± 0.38 μg/mL) detected in PB-BKT support the flavonoid-enriching effect of propolis (<i>p</i> &lt; 0.05). In another study, propolis supplementation of thermosonicated yellow cherry juice was found to increase quercetin content significantly. Additionally, the exclusive emergence of chrysin in the enriched samples, particularly its pronounced accumulation in BB-BKT (66.42 ± 1.84 μg/mL), suggests that bee bread supplementation can substantially reshape the specific phenolic signature of the matrix. This interpretation is further supported by the highest rosmarinic acid level observed in BB-BKT (3.05 ± 0.24 μg/mL). Conversely, the absence of caffeic acid and the flavones fraction across all treatments (n.d.) indicates that the applied interventions exerted limited effects on specific phenolic subclasses.</p><h5><strong>3.5. Free Amino Acids (LC–MS)</strong></h5><p>The PL-BKT sample, which was supplemented with pollen, exhibited the highest free amino acid content (see <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00008#tbl2">Table 2</a>). This outcome differed significantly from all other groups (<i>p</i> &lt; 0.05). Significant increases were observed in proline (47.37 mg/100 mL), leucine (4.10 mg/100 mL), and lysine (4.54 mg/100 mL), indicating that pollen, due to its high protein content, increases amino acid release during fermentation. In contrast, amino acid levels were lower in the sample supplemented with propolis alone (PB-BKT), and some amino acids (e.g., glutamic acid, histidine, threonine) were not statistically different from the BKT sample (<i>p</i> &gt; 0.05).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_8_14048_14063_Table_2_Effects_of_BKT_PB_BKT_PL_BKT_and_BB_BKT_on_Free_Amino_Acids_mg_100_m_L_f377c7c7e0.jpg" alt="ACS Omega 2026, 11, 8, 14048–14063: Table 2. Effects of BKT, PB-BKT, PL-BKT and BB-BKT on Free Amino Acids (mg/100 mL). The mean value, along with the standard deviation, is given for the data (n = 3). Values indicated by different letters within the same row differ significantly (p &lt; 0.05). BKT: Blackthorn kombucha tea; PB-BKT: Propolis blackthorn kombucha tea; PL-BKT: Pollen blackthorn kombucha tea; BB-BKT: Bee bread blackthorn kombucha tea." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_8_14048_14063_Table_2_Effects_of_BKT_PB_BKT_PL_BKT_and_BB_BKT_on_Free_Amino_Acids_mg_100_m_L_f377c7c7e0.jpg 159w," sizes="100vw" width="1055" height="1033"></p><h4><strong>4. Conclusions</strong></h4><p>This study demonstrated that kombucha enriched with <i>P. spinosa</i> L. juice and bee products (propolis, pollen, and bee bread) provides a robust model for functional beverage design through RSM-based optimization and advanced analytical characterization (HPLC–DAD, LC–MS/MS). From a sensory perspective, the highest overall acceptability was observed in the bee bread-enriched sample (8.32), which also exhibited the strongest antioxidant response (TPC 257.10 mg GAE/100 mL; TFC 36.16 mg CE/100 mL; DPPH• 57.05 μmol TE/mL). HPLC–DAD data revealed that total phenolics increased to 159.23–186.15 μg/mL following enrichment, and that marker compounds, such as chlorogenic acid and catechin derivatives, varied depending on the formulation. LC–MS/MS supported an increase in free amino acids (particularly proline) with pollen supplementation. Propolis addition highlighted the antidiabetic potential, yielding the highest enzyme inhibition (α-glucosidase: 40.7%; α-amylase: 42.02%). Although a partial reduction in bioactives was observed during in vitro digestion, the preservation of phenolic/flavonoid recovery within approximately the 21–30% range indicates that postdigestive activity may be sustained. Future studies are recommended to expand biomarkers using targeted/untargeted LC–HRMS-based metabolomic approaches, to correlate SCOBY microbiota (16S/ITS) with chemical profiles, and to evaluate shelf life and packaging stability (phenolic degradation kinetics, volatile profile), as well as in vivo/preclinical validation (glycemic response, bioaccessibility). A limitation of this work is that batch-specific botanical origin (palynological) identification of the commercial bee products was not performed; therefore, compositional variability due to botanical source cannot be fully excluded.</p></div>

From Chemistry to Functionality: HPLC–DAD/LC–MS/MS Characterization of Bee Product-Enriched Prunus spinosa L. Kombucha with In Vitro Antidiabetic Activity and Bioaccessibility

<p><strong>In the week of April 27, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Separation Science: Analytical Excellence Week 2026: Part 1- Understanding Pharmacopoeia and ACS Compliance</strong></h5><ul><li>Tu, 28.4.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6130">Registration</a></li></ul><p>Discover the critical role of compliant reagents in your lab and learn how to ensure that your analyses meet regulatory standards.</p><h5><strong>2. Agilent Technologies: PFAS &amp; Our Planet: Environmental Monitoring</strong></h5><ul><li>Tu, 28.4.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6140">Registration</a></li></ul><p>Explore evolving PFAS regulations and practical LC-MS/MS workflows for monitoring PFAS in water, soil, and air, including contamination control, sample prep, and reporting strategies.</p><h5><strong>3. Restek Corporation: Air Sampling Media Selection and Analysis</strong></h5><ul><li>Tu, 28.4.2026 14:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6160">Registration</a></li></ul><p>This presentation will give an overview of the selection of air sampling media, covering both whole air and sorbent sampling techniques.</p><h5><strong>4. Tosoh Bioscience: Tosoh Tuesday - What is MALS &amp; When is it Useful?</strong></h5><ul><li>Tu, 28.4.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6145">Registration</a></li></ul><p>Join Tosoh Tuesday to explore MALS fundamentals, practical implementation, and real-world SEC-MALS applications for polymer and biologics characterization.</p><h5><strong>5. Technology Networks: Mass Spectrometry in Drug Discovery: Covalent and Non-Covalent Screening Applications</strong></h5><ul><li>Tu, 28.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6114">Registration</a></li></ul><p>Identifying small-molecule binders for complex biological targets remains a major challenge in early drug discovery.</p><h5><strong>6. Thermo Fisher Scientific: From Sample to Structure: Crosslinking MS Made Practical - Part 1</strong></h5><ul><li>Tu, 28.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6115">Registration</a></li></ul><p>Crosslinking mass spectrometry (XL-MS) is transforming how researchers map protein–protein and protein–nucleic acid interactions.</p><h5><strong>7. SelectScience: Why researchers turn to reversed phase chromatography when purifying GLP-1</strong></h5><ul><li>Tu, 28.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6117">Registration</a></li></ul><p>Purifying synthetic peptides is a critical step in developing advanced biotherapeutics like GLP-1 receptor agonists.</p><h5><strong>8. Phenomenex: Fundamentals First Bio Series – SEC for Biologics</strong></h5><ul><li>We, 29.4.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6149">Registration</a></li></ul><p>Master SEC fundamentals for biopharma—covering principles, column selection, and troubleshooting with real-world applications in mAbs, AAVs, and nucleic acids.</p><h5><strong>9. SCIEX: Extending the power of LC-MS/MS: advanced workflows with QTRAP®</strong></h5><ul><li>We, 29.4.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6147">Registration</a></li></ul><p>Discover how QTRAP® workflows enhance LC-MS/MS performance with MS³ selectivity, improving sensitivity, reducing interference, and enabling confident analysis in complex matrices.</p><h5><strong>10. Metrohm: The Basics of Titration explained</strong></h5><ul><li>We, 29.4.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6129">Registration</a></li></ul><p>In this webinar, Robert Tobler, Senior Application Specialist Titration at Metrohm International Headquarters, will give you an introduction to the basics of titration.</p><h5><strong>11. Separation Science: Analytical Excellence Week 2026: Part 2 - Benefits of PFAS-Tested Consumables</strong></h5><ul><li>Th, 30.4.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6131">Registration</a></li></ul><p>Gain insights into the importance of PFAS-tested additives and solvents for reliable analysis and their impact on the quality of your results.</p><h5><strong>12. Evosep Biosystems: Next-Gen HCP Analysis in Biopharma</strong></h5><ul><li>Th, 30.4.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6151">Registration</a></li></ul><p>Discover how LC-MS is advancing biopharma workflows through scalable, high-throughput proteomics.</p><h5><strong>13. Bruker: Bridging Molecules and Genes: Integrating MALDI Imaging and Spatial Transcriptomics on a Single Tissue Section</strong></h5><ul><li>Th, 30.4.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6132">Registration</a></li></ul><p>Explore how integrating MALDI Imaging with spatial transcriptomics reveals tissue heterogeneity, molecular gradients, and disease-relevant patterns through advanced multimodal workflows.</p><h5><strong>14. Thermo Fisher Scientific: From Sample to Structure: Crosslinking MS Made Practical - Part 2</strong></h5><ul><li>Th, 30.4.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6116">Registration</a></li></ul><p>Crosslinking mass spectrometry (XL-MS) is transforming how researchers map protein–protein and protein–nucleic acid interactions.</p>

Webinars LabRulezLCMS Week 18/2026

Article

Webinars

Video

Scientists

Food

Scientific article

Interview

Science and research

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Thermo Scientific Orbitrap Exploris 480 Mass Spectrometer

Empowering life science researchers to translate each experimental step to a new level of insight with built-in workflows and single-cell sensitivity.

<p><strong>Empowering life science researchers to translate each experimental step to a new level of insight with built-in workflows and single-cell sensitivity. Accelerate your path to impactful results with a next-generation ownership experience coupled with an intelligence-driven experimental approach. Achieve a new standard in performance to study trace samples with extraordinary accuracy, certainty, confidence and simplicity. You'll find market leading quantitative performance to solve your most complex challenges with ease, and the proven qualitative performance you expect from Orbitrap- based mass spectrometry.</strong></p><p>From exploratory to targeted quantitation, find the power and reliability to make large scale studies possible, while reducing everyday hassles as you increase productivity. And achieving it all, remarkably, within a compact footprint. An extraordinary instrument, providing extraordinary results; the Thermo Scientific Orbitrap Exploris 480 mass spectrometer.</p><h4><strong>Key benefits of the Orbitrap Exploris 480 mass spectrometer</strong></h4><h5><strong>Next-generation experience</strong></h5><p>Built with next-generation common hardware and software architecture, the guiding principle of ultimate performance and ease of use delivers effortless access to the value contained within your data.</p><h5><strong>High performance, high-throughput insights</strong></h5><p>Adding greater performance and intelligence to market-leading selectivity and spectral quality imparts productivity, depth, and certainty to small and large- scale studies.</p><h5><strong>Robust performance and exceptional serviceability increase uptime</strong></h5><p>By design, exceptional end-to-end robustness minimizes need for performance-related maintenance, increasing uptime and sample throughput.</p><h5><strong>Ease-of-use and intelligence to empower all users</strong></h5><p>Differences in user expertise can be a challenge in many laboratories. Workflows based on intelligent data acquisition methods leverage new levels of instrument performance to deliver high confidence, and high throughput.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Ion_path_and_key_components_of_the_Orbitrap_Exploris_480_mass_spectrometer_3f5791378c.webp" alt="Thermo Fisher Scientific: Ion path and key components of the Orbitrap Exploris 480 mass spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Ion_path_and_key_components_of_the_Orbitrap_Exploris_480_mass_spectrometer_3f5791378c.webp 245w," sizes="100vw" width="950" height="410"></p><div><h4><strong>How does the Orbitrap Exploris 480 MS enable greater data insights faster?</strong></h4></div><div><p>See what makes the Orbitrap Exploris 480 MS stand out, how it can empower all users to obtain the highest quality results and deepest insights with the least amount of effort; see how our breakthrough is now your breakthrough.</p></div><ul><li><strong>Next-Generation Instrument Design</strong></li><li><strong>Targeted Protein Quantitation Workflows with SureQuant IS</strong></li><li><strong>Advancing Accuracy, and Precision in Quantitative Proteomics through Multiplexing</strong></li><li><strong>Sub 1-ppm Mass Accuracy as Standard</strong></li><li><strong>Improved Performance through Instrument Enhancements</strong></li><li><strong>Intelligence-Driven Instrument Set-Up, Control and Data Acquisition</strong></li><li><strong>Visualize the Orbitrap Exploris Data Acquisition Modes</strong></li></ul><h4><strong>Optional features to maximize performance</strong></h4><p>Maximize performance and flexibility to expand experimental capabilities of the Orbitrap Exploris 480 mass spectrometer with multiple hardware and software options, allowing you to see more, quantify more, deliver more confidence and greater insights with even more productivity.</p><ul><li><strong>Enhance your Experimental Performance with the FAIMS Pro Duo interface</strong></li><li><strong>Comprehensive and confident biotherapeutic characterization</strong></li></ul><div data-uw-styling-context="true"><h4><strong>Data analysis made easy</strong></h4></div><div data-uw-styling-context="true"><p data-uw-styling-context="true">From complex proteomic challenges, quantitation with SureQuant and TMT multiplexing, through to complete biotherapeutic characterization, data analysis doesn’t have to be the bottleneck to accessing deeper insights into your high-quality MS data. Despite the numerous workflows involved in the analysis of proteins, there are flexible analysis tools which allow simple, yet confident, interpretation of your data to streamline your research and facilitate decision making to take your research to the next level.</p></div><ul><li><strong>Flexibly Identify and Quantify Proteins Based on your Workflow</strong></li><li><strong>The Right Path Towards Complete Biotherapeutic Characterization</strong></li><li><strong>Compliance and Enterprise Readiness with Chromeleon CDS Software</strong><br>&nbsp;</li></ul>

Thermo Scientific Orbitrap Exploris 240 Mass Spectrometer

Maximize performance and flexibility to expand your experimental capabilities, allowing you to discover more, see more, quantify more, and deliver more confidence and greater insights with even more productivity.

<div container-id="cq-colctrl-lt8" comp-id="B2"><div><p><strong>Maximize performance and flexibility to expand your experimental capabilities, allowing you to discover more, see more, quantify more, and deliver more confidence and greater insights with even more productivity.</strong></p><p>Deliver on your objectives with performance to drive discovery and identification, plus receive the quantitative precision and accuracy you need to confidently scale up and achieve impact. With operational simplicity through intelligent data acquisition and processing, discover your fast track to confident results regardless of sample complexity, the depth of insights required or the presence of unknown compounds.</p><h4><strong>Application capabilities through instrument versatility</strong></h4><p>Maximize performance and flexibility to expand your experimental capabilities, allowing you to discover more, see more, quantify more, and deliver more confidence and greater insights with even more productivity.</p><ul><li><strong>Confident and comprehensive proteomic analyses</strong></li><li><strong>Translational proteomics: Scaling translational discoveries into reliable clinical applications</strong></li><li><strong>Comprehensive biotherapeutic characterization: Maximize biotherapeutic insights&nbsp;</strong></li><li><strong>Intact native mass analysis: Maximize biotherapeutic insights&nbsp;</strong></li><li><strong>Metabolomics workflows: One solution for all metabolics workflows&nbsp;</strong></li><li><strong>Clinical research: Translate qualitative and quantitative assays into future clinical applications</strong></li><li><strong>Food/beverage and environmental analyses: Fast-track to results with confidence&nbsp;</strong></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Thermo_Scientific_Orbitrap_Exploris_240_Mass_Spectrometer_a4d2d7771c.jpg" alt="Thermo Fisher Scientific: a) Quantitative experimental design for the analysis of a control and three knockout (KO) strains. b) The orthogonality of the FAIMS Pro interface shows improved peptide coverage when using a range of different CVs. c) Intensity ranking across five orders of quantitative dynamic range for the peptide spectrum matches provided by Proteome Discoverer software. d) Quantitative ratios for each of the samples, highlighting the relative abundance of the KO proteins." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Thermo_Scientific_Orbitrap_Exploris_240_Mass_Spectrometer_a4d2d7771c.jpg 245w," sizes="100vw" width="1000" height="543"></p><div><h4><strong>Key benefits of the Orbitrap Exploris 240 mass spectrometer</strong></h4></div><div><p>Delivering actionable insights faster than ever before by combining proven benefits of Thermo Scientific Orbitrap technology, with sub 1-ppm mass accuracy and high resolution, with intelligent data acquisition and processing.</p></div><h5><strong>Everyday experimental flexibility</strong></h5><p>Discover workhorse versatility from confident proteomic and small-molecule identification and quantitation to biopharmaceutical characterization and in-depth sample-profiling.</p><h5><strong>Faster path to confident insights</strong></h5><p>Delivering actionable insights faster than ever before by combining proven benefits of Thermo Scientific Orbitrap technology, such as sub 1-ppm mass accuracy and high resolution, with intelligent data acquisition and processing.</p><h5><strong>Reliable, robust, and maintainable</strong></h5><p>Meet an exceptional system every day, one with versatility and operational simplicity, but that doesn’t compromise robustness and reliability so you can be confident in its ability to perform on long-term studies.</p><h5><strong>Focus on driving results with faster setup</strong></h5><p>Fully integrated instrument control, setup, and data processing, helps you focus on delivering actionable results while single-system calibrations and pre-defined method templates drive productivity.</p><blockquote><h5><strong>Top reasons to choose Orbitrap Exploris 240 MS over Q-TOF instrumentation</strong></h5><p>Others claim to set new standards in proteomics. Is Protein ID without quantitation sufficient for shotgun proteomics to provide biological insights? Meet the Orbitrap Exploris 240 mass spectrometer – with leading quantitative performance, sensitivity and versatility, designed to meet your needs.</p><p>Trust us to deliver technology innovations that are foundations of reliable workflows and solutions, along with publishable and actionable results.</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/products-and-services/promotions/industrial/10-reasons-choose-orbitrap-exploris-over-qtof.html"><span><strong>Learn more</strong></span></a></li></ul></blockquote><h4><strong>Data Analysis with connectivity to drive productivity</strong></h4><p>From complex proteomic challenges, complete biotherapeutic characterization, or small-molecule discovery to everyday testing, data analysis is your gateway to accessing deeper insights into your high-quality MS and MS2 data. Despite the numerous workflows involved, there are flexible analysis tools for simple, confident interpretation of your data to streamline research and facilitate rapid decision making.</p><ul><li><strong>Flexibly identify and quantify proteins based on your workflow</strong></li><li><strong>Biopharmaceutical characterization with BioPharma Finder software</strong></li><li><strong>Streamline your small-molecule qualitative and quantitative assays</strong></li><li><strong>Small-molecule characterization with Compound Discoverer software</strong></li><li><strong>Complete screening and quantitation using Thermo Scientific TraceFinder software</strong></li><li><strong>Complete compliant control with Chromeleon CDS for MS</strong></li></ul></div></div>

Thermo Scientific Orbitrap Exploris 120 Mass Spectrometer

With its unique ability to provide consistently accurate data and a design made for operational simplicity, it sets the new standard in instrument productivity and ruggedness.

<p><strong>The proven Thermo Scientific Orbitrap technology in the Thermo Scientific Orbitrap Exploris 120 mass spectrometer accelerates your route to qualitative and quantitative confidence for both method development and everyday testing purposes. With its unique ability to provide consistently accurate data and a design made for operational simplicity, it sets the new standard in instrument productivity and ruggedness.</strong></p><h4><strong>Key benefits of the Orbitrap Exploris 120 mass spectrometer&nbsp;</strong></h4><h5><strong>Confidently perform high-throughput qualitative and quantitative analysis</strong></h5><p>Spend less time questioning your results with the unique ability of Orbitrap high-resolution, accurate-mass spectrometers (HRAM) to provide accurate results and reduce false positives or negatives. Confidently resolve ions of interest from interfering ions with high mass resolution up to 120,000 at m/z 200 for both method development and everyday testing analyses.</p><h5><strong>Keep your laboratory running seamlessly with the new standard in instrument ruggedness</strong></h5><p>Maximize return on your investment with capabilities that minimize downtime. Perform a single, stable calibration for the entire mass range from m/z 40-3000. With an improved scan rate up to 22 Hz, maximize your compound coverage with confidence. The ability to do both positive and negative mode switching ensures you analyze twice as many analytes in the same sample.</p><h5><strong>Focus on driving results with faster method set-up</strong></h5><p>Empower high-resolution MS users by giving them the ability to analyze samples using ready-to-run method templates. Minimize time spent on software training for Thermo Scientific MS users with a common software user interface for data acquisition.</p><h4><strong>How the Orbitrap Exploris 120 MS works</strong></h4><p>Discover how the Orbitrap Exploris 120 MS instrument enables you to prioritize your time on making critical decisions which impact the future of you or your client's organization. The latest instrument capabilities have been designed with the sole purpose of putting you on the fast path to accurate, reportable results. Leave the lab at the end of the day feeling productive and accomplished.</p><ul><li><strong>Uncover and quantify more suspect analytes</strong></li><li><strong>Sub-ppm mass accuracy as standard</strong></li><li><strong>System to system consistency</strong></li><li><strong>Robust performance</strong></li><li><strong>Maximize compound coverage with faster scan rates&nbsp;</strong></li><li><strong>Analyze samples twice as fast with rapid polarity switching&nbsp;</strong></li><li><strong>Intelligence-driven instrument setup, control and data acquisition&nbsp;</strong></li><li><strong>Quantitation: Which scan modes do I use on the instrument?</strong></li><li><strong>Take a video tour&nbsp;</strong></li></ul><div><h4><strong>Data analysis software: Streamline small molecule qualitative and quantitative assays</strong></h4></div><div><p>Connecting small molecule unknown identification and characterization through to screening and quantitation doesn’t just stop at data acquisition across multiple platforms. Now that you have acquired comprehensive, high-quality MS and MS<sup>2</sup> data, you need to identify and characterize unknowns and transfer discovery knowledge through to every day testing.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Which_data_analysis_software_is_right_for_you_30a173dd7f.jpg" alt="Thermo Fisher Scientific: Which data analysis software is right for you?" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Which_data_analysis_software_is_right_for_you_30a173dd7f.jpg 213w," sizes="100vw" width="1385" height="1016"></p><p>Our connected data processing software ecosystem streamlines interpretation of your high-confidence discovery data through powerful Thermo Scientific Compound Discoverer software with direct access to the world’s largest LC-MS<sup data-uw-styling-context="true">n</sup> reference spectral library, Thermo Scientific&nbsp;mzCloud, or through utilization of offline or proprietary libraries. These libraries act as your knowledge repository and allow direct transfer through to everyday screening and quantitation using Thermo Scientific&nbsp;TraceFinder software.</p><ul><li><strong>mzCloud Mass Spectral Library</strong></li><li><strong>Small molecule characterization with Compound Discoverer software</strong></li><li><strong>Complete screening and quantitation using TraceFinder software</strong></li><li><strong>Complete compliant control with Chromeleon CDS for MS</strong></li></ul><h4><strong>Common applications for Orbitrap Exploris 120 Mass Spectrometer</strong></h4><p>To perform method development and everyday testing with confidence, you need a liquid chromatography mass spectrometry (LC-MS) workflow solution that is relevant to your laboratory’s goals. The Orbitrap Exploris 120 MS instrument can be used for a variety of applications, including clinical research, environmental and food safety, forensic toxicology, and pharmaceutical analysis.</p><ul><li><strong>Clinical Research</strong></li><li><strong>Forensic Toxicology</strong></li><li><strong>Drug Screening and Confirmation</strong></li><li><strong>Environmental Emerging Contaminants</strong></li><li><strong>Pesticide Residues Analysis</strong></li><li><strong>Veterinary Drugs Residues Analysis</strong></li><li><strong>Pharmaceutical applications</strong></li></ul></div>

Thermo Scientific Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer

Thermo Scientific Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer confidently and routinely characterize, quantify, and confirm in a single analysis using a single mass spectrometer.

<p><strong>Take Quanfirmation™ to the next level. The Thermo Scientific™ Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer introduced the revolutionary concept of Quanfirmation. The Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ Mass Spectrometer takes Quanfirmation to the next level with increased performance and new options that enhance applications from confident DMPK qual/quan screening studies to characterization of intact monoclonal antibodies.</strong></p><h5><strong>Superior Quantitation</strong></h5><ul><li><strong>Advanced quadrupole technology (AQT)</strong> improves precursor selection and transmission for more-accurate quantitation of low-abundance analytes in complex matrices</li><li><strong>Sophisticated data-independent acquisition (DIA)</strong> and parallel reaction monitoring (PRM) deliver reproducible quantitation with complete qualitative confidence</li><li>An <strong>advanced active beam guide (AABG) </strong>reduces noise and extends maintenance intervals</li></ul><h5><strong>Enhanced Analysis of Intact Proteins</strong></h5><ul><li><strong>Optional intact protein mode</strong> provides superior trapping of large molecules for improved analysis of intact proteins and protein complexes</li><li><strong>Optional enhanced resolution </strong>ensures maximum ID confidence in top-down proteomics and lipidomics</li></ul><h5><strong>High-Confidence Results</strong></h5><ul><li><strong>HR/AM and full-scan capabilities </strong>capture all sample data, all of the time, enabling retrospective data analysis without need to re-run samples</li><li><strong>Resolving power up to 140,000 FWHM </strong>eliminates isobaric interferences, increasing confidence in results when analyzing samples in complex matrices</li><li><strong>Better than 1-ppm mass accuracy</strong> in full- and all-ion fragmentation (AIF) scan modes ensures confident compound identification</li><li><strong>Fast scanning at 12 Hz</strong> and spectral multiplexing suited to UHPLC applications</li><li><strong>Rapid polarity switching</strong> maximizes information obtained</li><li><strong>AIF and multiple dissociation techniques</strong> — in-source CID and HCD — aid in compound identification</li><li><strong>Mass range to 6000 m/z </strong>enhances detection of singly charged small molecules and biomolecules</li><li><strong>More than four orders of magnitude</strong> intrascan dynamic range, along with femtogram-level sensitivity, allow detection of trace-level and high-abundance compounds in the same scan</li></ul>

Thermo Scientific™ Q Exactive™ UHMR Hybrid Quadrupole Orbitrap™ Mass Spectrometer

Thermo Scientific Q Exactive UHMR Hybrid Quadrupole-Orbitrap MS offers ultra-high mass range native MS with top-down capabilities, extreme sensitivity, and resolution up to 80,000 m/z.

<h3><strong>Go beyond what you thought possible with native mass spectrometry</strong></h3><p>The Thermo Scientific™ Q Exactive™ UHMR is the first hybrid quadrupole-Orbitrap™ mass spectrometer designed specifically for ultra-high mass range (UHMR) native MS and top-down protein analysis. It combines exceptional sensitivity, ultra-high resolution at high m/z, and unique pseudo-MS³ capabilities, enabling detailed characterization of intact protein assemblies and complex biological systems.</p><h3>🔬 <strong>Breakthrough in Native Mass Spectrometry</strong></h3><p>Native mass spectrometry preserves the non-covalent structure and interactions of proteins and protein complexes, making it an indispensable tool for structural biology and biopharma. The Q Exactive UHMR system enables direct mass analysis of intact macromolecules, including membrane proteins, virus capsids, and ribosomal particles—with unrivaled detail.</p><ul><li><strong>Ultra-high mass range detection</strong> up to <strong>80,000 m/z</strong></li><li><strong>Native top-down analysis</strong> of protein complexes and assemblies</li><li><strong>High sensitivity</strong> for precious or low-abundance samples</li><li><strong>Resolve mass differences</strong> even in heterogeneous megaDalton assemblies</li></ul><h3>⚙️ <strong>Technological Innovations</strong></h3><p>The Q Exactive UHMR system integrates multiple hardware advancements to overcome the historical limitations of native MS:</p><ul><li><strong>In-source trapping</strong> for efficient desolvation and controlled dissociation</li><li><strong>Advanced quadrupole mass filter</strong> with selection up to <strong>25,000 m/z</strong></li><li><strong>Reduced RF frequencies</strong> across the ion path for better high-mass transmission</li><li><strong>Pseudo-MS³ capability</strong>: fragment large complexes into subunits, then sequence them in HCD cell</li></ul><h3>🧬 <strong>Applications and Capabilities</strong></h3><h4><strong>Confident characterization of complex biological systems:</strong></h4><ul><li><strong>Virus capsid analysis:</strong> Differentiation and sequencing of 3–4 MDa hepatitis B virus capsids</li><li><strong>Ribosome studies:</strong> Resolution of substoichiometric proteins in E. coli 70S particles</li><li><strong>Membrane proteins:</strong> Gentle liberation from micelles and native top-down sequencing (e.g., AmtB trimer)</li><li><strong>Chaperonin complexes:</strong> Native top-down analysis of GroEL tetradecamer</li></ul><h4><strong>Detect and resolve small differences in high-mass species:</strong></h4><ul><li>Post-translational modifications</li><li>Ligand binding</li><li>Protein truncations and isoforms</li></ul><h3>💡 <strong>Software for Confident Data Interpretation</strong></h3><p>Maximize the power of native MS with industry-leading software:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1837"><strong>BioPharma Finder™ Software</strong> </a>– For intact mass confirmation, top-down analysis, and PTM localization</li><li><strong>ProSightPC™ Software</strong> – Advanced top-down proteomics with integrated UniProt database search</li></ul><h3>🧪 <strong>Who Is It For?</strong></h3><p>Ideal for research groups and industry labs focused on:</p><ul><li>Structural biology</li><li>Biotherapeutic development</li><li>Native and top-down proteomics</li><li>Protein-ligand interaction studies</li></ul><h3>🧭 <strong>Summary of Key Features</strong></h3><ul><li><strong>Mass range:</strong> Up to 80,000 m/z</li><li><strong>Quadrupole isolation:</strong> Up to 25,000 m/z</li><li><strong>Sensitivity:</strong> Orders of magnitude higher at high m/z</li><li><strong>Fragmentation:</strong> HCD, pseudo-MS<sup>3</sup> for native top-down</li><li><strong>In-source trapping:</strong> Yes, optimized for membrane protein assemblies</li><li><strong>Software:</strong> BioPharma Finder, ProSightPC</li><li><strong>Compatible analyses:</strong> Native MS, top-down, intact mass, charge detection</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Q_Exactive_UHMR_Hybrid_Quadrupole_Orbitrap_hmotnostni_spektrometr_a7f594a56d/Pragolab-Thermo-Q-Exactive-UHMR-Hybrid-Quadrupole-Orbitrap-hmotnostni-spektrometr.webp" alt="Thermo Scientific™ Q Exactive™ UHMR Hybrid Quadrupole Orbitrap™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Q_Exactive_UHMR_Hybrid_Quadrupole_Orbitrap_hmotnostni_spektrometr_a7f594a56d/thumbnail_Pragolab-Thermo-Q-Exactive-UHMR-Hybrid-Quadrupole-Orbitrap-hmotnostni-spektrometr.webp 156w," sizes="100vw" width="650" height="650"></p>

Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer

Orbitrap Fusion Tribrid MS combines quadrupole, ion trap, and Orbitrap analyzers for ultra-flexible MSⁿ workflows, enabling deep proteomics, PTM analysis, and confident structural elucidation.

<h4><strong>Revolutionary tribrid architecture for comprehensive structural analysis and deep proteome coverage</strong></h4><p>The Orbitrap Fusion Tribrid mass spectrometer combines a quadrupole, a linear ion trap, and an Orbitrap mass analyzer in a unique architecture that delivers unmatched analytical performance. It provides life scientists with advanced MS<sup>n</sup> capabilities, flexibility in fragmentation techniques, and exceptional resolution and speed — ideal for tackling the most complex analytical challenges in proteomics, PTMs, metabolomics, lipidomics, and small molecule research.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_e93c577ae5/Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_e93c577ae5/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr.webp 245w," sizes="100vw" width="1914" height="1059"></p><h3><strong>Key Features and Benefits</strong></h3><h4>🔬 <strong>Revolutionary Tribrid Design</strong></h4><ul><li>Combines <strong>quadrupole</strong>, <strong>linear ion trap</strong>, and <strong>Orbitrap</strong> analyzers</li><li>Perform <strong>MSⁿ up to n = 10</strong> with detection in either ion trap or Orbitrap</li><li>Fragmentation options: <strong>CID</strong>, <strong>HCD</strong>, <strong>ETD</strong>, and <strong>EThcD</strong> at any MS stage</li><li><strong>Synchronous Precursor Selection (SPS)</strong>: up to <strong>20 precursors per scan</strong> for MS³</li></ul><h4>⚙️ <strong>High Performance &amp; Resolution</strong></h4><ul><li><strong>Resolving power up to 500,000 FWHM</strong> at m/z 200</li><li><strong>&lt;1 ppm</strong> mass accuracy with EASY-IC™ internal calibration</li><li><strong>Polarity switching in 1.1 s full cycle</strong></li><li><strong>Scan speeds up to 20 Hz</strong> for Orbitrap and ion trap MSn</li></ul><h4>🧪 <strong>Versatility in Applications</strong></h4><ul><li>Analyze <strong>low-abundance</strong>, <strong>high-complexity</strong>, or structurally complex samples</li><li>Confidently resolve <strong>isobaric interferences</strong> and detect <strong>PTMs</strong></li><li>Full compatibility with <strong>intact protein analysis</strong>, glycoproteomics, and TMT SPS-MS³ quantitation workflows</li></ul><h4>🔁 <strong>Efficient Ion Handling</strong></h4><ul><li><strong>EASY-Max NG</strong> or <strong>NanoSpray Flex NG</strong> ion sources</li><li><strong>Active Beam Guide</strong> and <strong>S-Lens</strong> for efficient ion transmission</li><li><strong>Dynamic Scan Management</strong> optimizes scan scheduling and parallelization</li></ul><h4>💡 <strong>Ease of Use</strong></h4><ul><li>Intuitive <strong>Tune and Method Editors</strong></li><li>Method templates for routine and advanced workflows</li><li><strong>Quanfirmation</strong>: HRAM SIM for quantitation + ion trap MS/MS for confirmation</li><li>Automated calibration, AGC, and exclusion logic</li></ul><h4>🧬 <strong>Compatible Software</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758"><strong>Xcalibur™</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782"><strong>Proteome Discoverer™</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1756"><strong>ProSight™</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1757"><strong>Mass Frontier™</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759"><strong>Compound Discoverer™</strong></a>, and others</li><li>Support for data-dependent, targeted, and deconvolution workflows</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_2_3d7accb56e/Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr-2.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_2_3d7accb56e/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr-2.webp 245w," sizes="100vw" width="1038" height="622"></p>

Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer

The Orbitrap Fusion Lumos Tribrid MS combines quadrupole, ion trap, and Orbitrap analyzers for high-resolution, ultra-sensitive MSn workflows in proteomics, metabolomics, and biopharma.

<h4><strong>The most powerful tribrid MS platform for deep, multidimensional analysis in proteomics, metabolomics, and structural biology</strong></h4><p>The <strong>Orbitrap Fusion Lumos Tribrid MS</strong> is a cutting-edge mass spectrometer that combines a quadrupole, a linear ion trap, and an Orbitrap™ high-resolution accurate-mass analyzer in a single platform. With unmatched sensitivity, selectivity, and versatility, it enables scientists to tackle complex biological questions with confidence—whether in discovery proteomics, targeted quantitation, top-down protein analysis, glycoproteomics, lipidomics, or small molecule research.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_1_39be5478b4/Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-1.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_1_39be5478b4/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-1.webp 245w," sizes="100vw" width="1823" height="1068"></p><h3>🔬 <strong>Unmatched Analytical Performance</strong></h3><ul><li><strong>&gt;500,000 resolution</strong> with Orbitrap Ultra-High Field analyzer</li><li><strong>Sub-ppm mass accuracy</strong> with real-time calibration</li><li><strong>Attomole-level quantification</strong> using PRM and DIA</li><li><strong>High sensitivity MS/MS</strong> with HCD, CID, ETD, EThcD, and new ETD HD fragmentation</li></ul><h3>🔄 <strong>Flexibility Across Workflows</strong></h3><ul><li><strong>Tribrid architecture</strong>: combine quadrupole precursor selection, ion trap MSn, and Orbitrap detection</li><li><strong>DIA, PRM, and SPS-MS3</strong> quantification workflows with isobaric tags (e.g., TMT, TMTpro)</li><li><strong>Top-down intact protein analysis</strong> with high sequence coverage</li><li><strong>Fast polarity switching</strong> and intelligent acquisition for lipidomics</li></ul><h3>🧪 <strong>Ideal for Multi-Omics and PTMs</strong></h3><ul><li>Deep profiling of <strong>glycopeptides</strong>, <strong>phosphoproteins</strong>, and other post-translational modifications</li><li>Resolution of <strong>isobaric isoforms</strong>, methylation patterns, and histone modifications</li><li>Confident structure elucidation of small molecules with fine isotope resolution</li></ul><h3>💡 <strong>Smart Technology for Productivity</strong></h3><ul><li><strong>Advanced Quadrupole Technology</strong> (AQT) for high transmission and selectivity</li><li><strong>Automatic gain control (AGC)</strong> for optimal ion population</li><li><strong>Easy ETD™ source</strong> and <strong>vent-free maintenance</strong> with source plug</li><li><strong>Method templates and visual method editor</strong> for intuitive setup</li></ul><h3>🧬 <strong>Applications</strong></h3><ul><li>Quantitative and discovery proteomics (e.g., large-scale TMT SPS-MS3)</li><li>Structural characterization of antibodies and proteoforms</li><li>Targeted metabolite quantitation and small molecule identification</li><li>Comprehensive glycoproteomics and lipidomics workflows</li></ul><h3>🧷 <strong>Software Compatibility</strong></h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758"><strong>Xcalibur™</strong></a> and <strong>Tune</strong> for control and calibration</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782"><strong>Proteome Discoverer™</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1756"><strong>ProSight™</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77"><strong>TraceFinder™</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1757"><strong>Mass Frontier™</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759"><strong>Compound Discoverer™</strong></a> for data analysis across omics</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_3_7458a4eb26/Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-3.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_3_7458a4eb26/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-3.webp 245w," sizes="100vw" width="2578" height="1296"></p>

Products focused on LC/Orbitrap from Thermo Fisher Scientific - page 2

Related content

Webinars LabRulezLCMS Week 18/2026

From Chemistry to Functionality: HPLC–DAD/LC–MS/MS Characterization of Bee Product-Enriched Prunus spinosa L. Kombucha with In Vitro Antidiabetic Activity and Bioaccessibility

Drug Discovery Where the Market Doesn’t Go: Targeting Neglected & Opportunistic Pathogens