<p><strong>An international research team, which included scientists from the Faculty of Science at Palacký University and the Institute of Physics of the Czech Academy of Sciences, has succeeded in unraveling the complex mechanism by which a chemical bond is formed during a collision between molecules. The experts found that the reaction only takes place if the molecules are oriented towards each other within a very narrow range of angles – within the so-called cone of reaction. This joint research has thus provided an answer to one of the fundamental questions of chemistry: under what conditions do chemical bonds form between molecules. The results of the study&nbsp;</strong><i><strong>Spatially resolving the cone of reaction for a single molecule</strong></i><strong>&nbsp;were published in the prestigious scientific journal&nbsp;Science.</strong></p><p>Many chemical reactions begin with the collision of two particles. However, for a new chemical bond to form, it is not enough for the molecules simply to come into contact. Their collision must occur with sufficient energy, in the correct relative orientation and at the right location. Under normal conditions, such as in solutions or gases, molecules collide chaotically, and the precise parameters of these collisions cannot therefore be controlled.</p><h4><strong>Molecules under control</strong></h4><p>Scientists were able to control the collisions of individual molecules on a copper surface and monitored the process using a <strong>scanning tunnelling microscope</strong>. They used the instrument not only to visualise individual molecules, but also to deliberately accelerate the reactants towards one another. They subsequently analysed each collision with atomic-level resolution. This enabled them to determine unequivocally whether a new chemical bond had formed between the molecules.</p><h5><i><strong>“What is hidden in the statistics of billions of collisions in conventional chemistry, we have managed to break down into individual cases and map directly.” Adam Matěj</strong></i></h5><p>A key finding of the study is that the outcome of a collision is determined by the extremely precise orientation of the molecules. A new bond was formed only when the target molecule was oriented within a few degrees of the direction of movement of the other molecule. When rotated by more than fifteen degrees, the molecules simply bounced off each other without any reaction.</p><h4><strong>The precise orientation of the molecules is crucial</strong></h4><p><i>“For the first time, we were able to observe, using a single molecule, exactly how precisely it must be oriented for the reaction to take place at all. What is hidden in the statistics of billions of collisions in conventional chemistry, we have managed to break down into individual cases and map directly,” </i>said Adam Matěj from the Department of Physical Chemistry at the Faculty of Science.</p><p>The theoretical part of the study, in which researchers from<strong> Palacký University </strong>and <strong>the Institute of Physics of the Czech Academy of Sciences</strong> were involved, played a significant role. Using quantum-mechanical calculations, they explained why the ‘reaction cone’ is so narrow. It turned out that the reactive carbon atom is surrounded by hydrogen atoms, which block the access of the reacting molecule from almost all directions.</p><h4><strong>The arrangement of atoms restricts access to the reaction</strong></h4><p>The calculations also shed light on a surprising experimental result. The chemical reaction took place even when the molecule moved along an adjacent row of copper atoms, where, according to the original assumptions, no bond should have formed.</p><h5><i><strong>“The strength of this work lies in the fact that the experiment and theory have been brought together into a single picture. The microscope showed what happens during the collision, and the calculations explained why.” Pavel Jelínek</strong></i></h5><p><i>“The explanation lies in the slight displacement of a single copper atom on the surface. The target’s bond to the surface pulls it out of its position and brings it close enough to the neighbouring row that it can literally take over the projectile and direct it to the correct spot. This shows that the surface is not merely a passive substrate, but actively participates in the reaction itself and can even facilitate pathways that would otherwise be unfavourable,” </i>noted Adam Matěj.</p><h4><strong>The surface also determines the course of the reaction</strong></h4><p>The results are significant for the development of so-called surface reactions, in which new materials and nanostructures are purposefully assembled from individual molecules. A better understanding of the influence of the spatial arrangement of molecules will enable the design of molecular building blocks so that they bond more reliably and efficiently. This represents an important step towards the development of technologies based on the controlled construction of structures atom by atom.</p><p><i>“The strength of this work lies in the fact that experiment and theory have been brought together into a single picture. The microscope showed what happens during the collision, and the calculations explained why. Without a theoretical model, we would have found it difficult to discover that the movement of a single surface atom is behind the unexpected course of the reaction,” </i>added Pavel Jelínek from the Institute of Physics of the Czech Academy of Sciences.</p><blockquote><p>The study&nbsp;<i>Spatially resolving the cone of reaction for a single molecule</i>&nbsp;was produced in collaboration between researchers from the University of Graz, the Institute of Physics of the Czech Academy of Sciences, Palacký University in Olomouc, Charles University and Humboldt University in Berlin.</p></blockquote>

Scientists reveal when chemical bonds form in molecular collisions 

<p><strong>In our </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7741"><strong>previous article, we introduced the main types of detectors used in HPLC</strong></a><strong>. This time, we take a closer look at the performance characteristics of LC detectors and the specifications that should be considered when assessing whether a detector is suitable for a particular chromatographic separation. We will also compare the most common detector technologies and provide some practical guidance for choosing the right option for a specific application.</strong></p><h3><strong>What Defines an Ideal HPLC Detector?</strong></h3><p>With such a wide variety of HPLC detectors available, an obvious question arises: why is there no single detector that works for every application? The simple answer is that no detector can satisfy every analytical requirement at the same time. Nevertheless, most detector designs aim to achieve a similar set of desirable properties.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Detector_requirements_334605c913.webp" alt="KNAUER: Figure 1 - Detector requirements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Detector_requirements_334605c913.webp 245w," sizes="100vw" width="1024" height="384"></p><h4><strong>1. High Sensitivity – Detecting Very Small Amounts</strong></h4><p>An ideal detector should be capable of measuring very low quantities of analyte, often in the nanogram or even picogram range. High sensitivity leads to lower limits of detection (LOD), can reduce sample preparation requirements, and is particularly valuable for applications such as impurity analysis or pharmacokinetic studies.</p><p>Some detector technologies, including fluorescence detection (FLD), electrochemical detection (ECD), and mass spectrometry (MS), can achieve extremely high sensitivity. However, this level of performance is often limited to specific classes of compounds.</p><h4><strong>2. Good Selectivity or Broad Applicability – Knowing What Is Being Detected</strong></h4><p>Selectivity describes how effectively a detector responds to the target analyte while minimizing interference from the sample matrix or mobile phase.</p><p>Depending on the analytical task, a detector may need to be highly selective, responding only to compounds with specific characteristics such as fluorescence or electrochemical activity. In other cases, broader applicability may be more important, allowing a wide range of compounds to be detected regardless of their molecular structure.</p><h4><strong>3. Linear Response and Broad Dynamic Range – Reliable Quantification</strong></h4><p>A good detector should provide a linear response across a sufficiently wide concentration range. This makes it possible to accurately quantify both major components and trace impurities within the same analytical method.</p><p>A broad linear range simplifies calibration and improves confidence in quantitative results. Detector response should also remain highly reproducible from one injection to another.</p><h4><strong>4. Compatibility with HPLC Conditions – Fitting the Chromatographic Method</strong></h4><p>An ideal detector should be compatible with both gradient and isocratic elution, commonly used mobile phases and additives, typical flow rates, and standard HPLC operating temperatures.</p><p>Poor compatibility may restrict method flexibility or force compromises in chromatographic performance. Some detectors provide excellent analytical performance but only under specific operating conditions, making them less suitable for certain methods.</p><h4><strong>5. Stability and Low Noise – Distinguishing Signal from Background</strong></h4><p>A stable baseline with minimal noise and drift improves the signal-to-noise ratio and overall data quality. This becomes especially important in long chromatographic runs, gradient methods, and trace-level analysis.</p><p>Lower baseline noise makes weak analyte signals easier to distinguish and directly contributes to improved detection limits.</p><h4><strong>6. Robustness, Ease of Use, and Maintenance – Supporting Routine Laboratory Work</strong></h4><p>For day-to-day use, a detector should be reliable over long sequences, straightforward to operate, and require as little maintenance as possible.</p><p>In routine laboratories, robustness and ease of operation can be just as important as analytical performance. Simple troubleshooting, low maintenance requirements, and limited consumable costs are major advantages, particularly in high-throughput environments.</p><h3><strong>The Reality: No Detector Offers Everything</strong></h3><p>Since no detector fulfils all these requirements perfectly, detector selection is inevitably a compromise between sensitivity, selectivity, universality, robustness, operating flexibility, and cost.</p><p>For this reason, multi-detector configurations are increasingly common in modern HPLC systems. Combinations such as UV + CAD or UV + MS make it possible to benefit from complementary detection principles within a single analytical workflow.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_Comparison_of_commonly_used_HPLC_detector_classes_d74817b0f0.jpg" alt="KNAUER: Table 1 - Comparison of commonly used HPLC detector classes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_Comparison_of_commonly_used_HPLC_detector_classes_d74817b0f0.jpg 113w," sizes="100vw" width="806" height="1115"></p><h3><strong>Critical Performance Parameters of HPLC Detectors</strong></h3><h4><strong>Data Sampling Rate</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Example_for_too_less_and_too_high_data_sampling_rate_741a53edc6.webp" alt="KNAUER: Figure 2 - Example for too less and too high data sampling rate." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Example_for_too_less_and_too_high_data_sampling_rate_741a53edc6.webp 245w," sizes="100vw" width="1024" height="410"></p><p>The data acquisition rate, also known as the sampling rate, is one of the most important detector settings in HPLC. It describes how many data points are recorded per second and directly influences peak representation, peak shape, area precision, and baseline noise.</p><p>The higher the sampling rate, the more data points are collected. The detector processes the measurements collected over a defined time interval and averages them, and these averaged points are then used to construct the chromatogram.</p><p>At a data rate of 5 Hz, for example, five data points are acquired every second. If too few points are collected across a chromatographic peak, the resulting peak can appear jagged, poorly defined, and analytically unreliable, as illustrated on the left side of Figure 2.</p><p>At the other extreme, a setting such as 100 Hz records 100 points each second, which is often unnecessarily high for conventional HPLC. The optimum sampling rate mainly depends on chromatographic peak width.</p><p>For most standard HPLC applications, a data rate of approximately <strong>10 to 20 Hz</strong> is sufficient to generate a smooth and symmetrical Gaussian peak.</p><p>One consequence of using an excessively high acquisition rate is increased baseline noise. Statistically, the noise of the system depends on the number of data points used for averaging and decreases with the square root of the number of averaged measurements.</p><p>Selecting an appropriate sampling rate is therefore a balance between collecting enough points to describe the chromatographic peak accurately and avoiding unnecessary noise.</p><h4><strong>Noise and Drift</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Baseline_noise_and_drift_685652e641.webp" alt="KNAUER: Figure 3 - Baseline noise and drift" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Baseline_noise_and_drift_685652e641.webp 245w," sizes="100vw" width="1024" height="400"></p><p>What exactly is baseline noise? In simple terms, it represents the short-term fluctuations of the detector baseline over a defined period and is expressed in the units appropriate for the particular detector.</p><p>Baseline noise is typically determined from the peak-to-peak variation of the detector signal while the mobile phase passes through the system.</p><p>All HPLC detectors exhibit a certain level of noise. Potential sources include:</p><ul><li>electronic noise,</li><li>detector cell noise,</li><li>detector instability,</li><li>temperature fluctuations,</li><li>mobile-phase effects,</li><li>and other environmental or instrumental influences.</li></ul><p>Noise directly affects detector sensitivity because it determines the <strong>signal-to-noise ratio (S/N)</strong>.</p><p>A chromatographic peak is generally considered reliably detectable when its height is at least three times greater than the calculated baseline noise. This relationship is commonly used to define the <strong>limit of detection (LOD)</strong>.</p><p>The <strong>limit of quantification (LOQ)</strong> requires a stronger signal and is typically defined as approximately ten times the baseline noise.</p><p>Another important parameter affecting sensitivity is baseline drift. Drift reflects the gradual change in detector response over time and therefore provides useful information about long-term detector stability.</p><p>It is helpful to distinguish between short-term peak-to-peak noise and longer-term baseline drift over a defined time interval. Drift can be described as the average slope or change of the baseline within a specific period.</p><p>Excessive drift may reduce sensitivity and complicate chromatographic peak integration.</p><p>Short-term baseline instability can be influenced by contamination or deterioration of detector components, while long-term drift is often related to temperature stability, detector equilibration, or changes occurring within the analytical system.</p><h3><strong>How to Select the Right HPLC Detector</strong></h3><p>There is no universally “best” HPLC detector. The correct detector is simply the one that best matches the analytical task.</p><p>When selecting a detector, several practical questions can help narrow the choice.</p><h4><strong>1. Does the analyte absorb UV light or fluoresce?</strong></h4><p>If the answer is yes, optical detectors such as <strong>UV/VIS</strong> or <strong>FLD</strong> are often the simplest and most effective options.</p><p>UV/VIS detection is widely applicable to compounds containing suitable chromophores, while fluorescence detection provides particularly high sensitivity and selectivity for naturally fluorescent or derivatized analytes.</p><h4><strong>2. Does the analyte lack a chromophore?</strong></h4><p>If UV absorption is weak or absent, detectors such as <strong>RID, ELSD, or CAD</strong> may provide a more appropriate solution.</p><p>These detector types are useful for compounds such as sugars, lipids, polymers, and other analytes that are difficult to monitor by conventional UV detection.</p><h4><strong>3. Is structural information required?</strong></h4><p>If structural information is important, <strong>mass spectrometry</strong> is usually the strongest choice.</p><p>MS provides mass-to-charge information and, when tandem mass spectrometry is used, fragmentation data that can support compound identification and structural characterization.</p><h4><strong>4. Is extremely high sensitivity necessary?</strong></h4><p>For applications requiring very low detection limits, <strong>electrochemical detection</strong> or <strong>mass spectrometry</strong> may be the most suitable options.</p><p>The exact choice depends on the chemical properties of the analyte and the required degree of selectivity.</p><h4><strong>5. Are macromolecules such as proteins or polymers being investigated?</strong></h4><p>For characterization of macromolecules, <strong>MALS</strong> and <strong>viscometry</strong> can provide valuable information about molecular weight, molecular size, branching, and aggregation.</p><p>These techniques are especially powerful when combined with size-exclusion chromatography and can provide information that is not based solely on column elution behavior.</p><p>For a more detailed discussion of detector selection, see the KNAUER blog article <i>“The way you look at it – A comprehensive guide for selecting the appropriate HPLC detector.”</i></p><p>Choosing the detection technology based on analyte properties, required sensitivity, chromatographic conditions, and the type of information needed is essential for obtaining reliable and meaningful HPLC results.</p><h3><strong>Final Thoughts</strong></h3><p>Understanding the desired characteristics and key performance parameters of HPLC detectors helps explain why different detector technologies are suited to different applications.</p><p>No single detector can provide maximum sensitivity, selectivity, universality, robustness, flexibility, and low operating cost simultaneously. Recognizing these trade-offs provides a practical framework for selecting the most appropriate detector for a given chromatographic method.</p><p>Parameters such as data sampling rate, baseline noise, drift, dynamic range, and compatibility with chromatographic conditions are just as important as the underlying detection principle itself.</p><p>In the next articles of this series, we will take a closer look at the individual HPLC detection technologies, beginning with the most widely used optical detection method: <strong>UV/VIS detection</strong>.</p>

Seeing is Believing – Detection Made Easy Part 2: Detector Characteristics

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 17th August 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you posters by <strong>Agilent Technologies / ASMS, Thermo Fisher Scientific / ASMS </strong>and<strong> Shimadzu / ASMS </strong>and application note by<strong> Waters Corporation!</strong></p><h3><strong>1. Agilent Technologies / ASMS: Quantitation of N-Nitroso Duloxetine N-Nitrosamine Drug Substance Related Impurities (NDSRI) in API and Formulation by 6475 LC/TQ</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_drug_impurities_lc_tq_tp_402_asms_2026_en_agilent_b933b9bddf.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Nitrosamine impurities are nitroso derivatives where the nitroso group is attached to an amine. The active pharmaceutical ingredient (API) derived from a class of nitrosamines are known as N-nitrosamine drugsubstance-related impurities (NDSRI). Nitrosamine impurities in APIs and drug products pose safety concerns, even in small quantities, and thus are a major concern for drug makers [1].&nbsp;</p><p>Analytical methods must be sufficiently sensitive to quantify the nitrosamine of interest at levels as low as 10% of the specification limit and, where feasible, down to ≤5% of the specification limit, for the determination of the limit of detection. LC/MS/MS is an inherently selective and highly sensitive analytical technique that is well suited for the identification and quantification of trace-level impurities at extremely low concentrations. Consequently, LC/MS/MS has been widely adopted within the pharmaceutical industry, particularly for nitrosamine analysis [2-3].&nbsp;</p><p>Therefore, manufacturers must develop and qualify a dedicated analytical method that is appropriate for the specific sample matrix and targeted NDSRIs. An LC/MS/MS method based on multiple reaction monitoring (MRM) was developed for the detection and quantification of the N-nitroso duloxetine NDSRI impurity over a concentration range of 0.5 to 40 ppb (absolute standard concentration), using an atmospheric pressure chemical ionization (APCI) source on an Agilent 6475A LC/TQ instrument.</p><h4><strong>Experimental</strong></h4><ul><li><strong>MS:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6475"><strong>6475 Triple Quadrupole LC/MS System</strong></a></li><li><strong>Column:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=Poroshell+HPH+C18"><strong>Poroshell HPH C18</strong></a> (4.6*150mm, 2.7µm)</li></ul><h4><strong>Conclusions</strong></h4><ul><li>A highly sensitive and robust MRM LC/MS/MS method was developed for the quantitation of N-nitroso duloxetine in duloxetine hydrochloride API and finished formulation using the Agilent 6475A LC/TQ.&nbsp;</li><li>The optimized chromatographic conditions provided effective separation of the analyte from the API, placebo, and formulation matrices, ensuring reliable quantitation.&nbsp;</li><li>The calibration curve was linear over the concentration range of 0.5-40 ng/mL, with 1/x weighting. R and R2 values were observed for both to be greater than 0.99.&nbsp;</li><li>Recovery studies demonstrated efficient sample extraction, with recoveries ranging from 92-99% in API samples and 109-116% in pellet formulations at the LOQ, specification, and 200% specification levels.&nbsp;</li><li>The method also showed excellent reproducibility across all evaluated concentration levels, confirming its suitability for routine analysis.</li></ul><h3><strong>2. Shimadzu / ASMS: Non-Targeted Analysis of Per- and Polyfluoroalkyl Substances in Environmental Extracts by Microflow LC-QToF Coupled with Multi-spray ESI</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/TP_562_Non_Targeted_Analysis_of_Per_and_Polyfluoroalkyl_Substances_in_Environmental_Extracts_by_Microflow_LC_Q_To_F_Coupled_with_Multi_spray_ESI_c2a7e1d5bf.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Targeted screening methods are well established for monitoring Perand polyfluoroalkyl substances (PFAS) in environmental samples. However, PFAS comprise a large and evolving class of compounds, creating a need to screen existing, emerging, and transformation products across diverse matrices. Non-targeted Analysis (NTA) allows for the confirmation of suspected PFAS in the environment, but the sample characterization is often limited by analytical sensitivity. Microflow (0.5 – 40 µL/min) coupled with a multi-nozzle emitter enhances sensitivity by improving desolvation. When this is coupled to a high mass accuracy and resolution Quadrupole Time-ofFlight Mass Spectrometer (QToF), capable of data-dependent and independent MS/MS, PFAS characterization of unknown analytes is possible. In this study, various environmental extracts were processed and screened using NTA for PFAS compounds.</p><h4><strong>Methods</strong></h4><p>A <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=Nexera+Mikros"><strong>Shimadzu Nexera Mikros LC</strong> </a>was used to introduce the sample to a Newomics DuoESI source with an M3 emitter on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=9050"><strong>LCMS-9050 QToF MS</strong></a> (Fig. 1). A direct injection on an octadecyl column without delay column was performed. Direct injection was chosen over trapand-elute configuration to minimize selectivity bias caused by the trap column.&nbsp;</p><h4><strong>Conclusion</strong></h4><p>With the increased sensitivity of microflow LC-QToF coupled with multi-spray ESI with DIA acquisition, Insight Explore aided in NTA PFAS analysis. When paired with Insight Discovery and Profiler, a streamlined batch data processing workflow with statistical analysis was shown to effectively handle complex environmental matrices and their analyses.</p><h3><strong>3. Thermo Fisher Scientific / ASMS: Robust and sensitive quantitation of 18 steroids in human serum using Thermo Scientific TSQ Certis Triple Quadrupole mass spectrometer</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004707_lsms_18_steroids_tsq_certis_clinical_po004707_asms2026_na_en_244c52437b.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Accurate quantification of endogenous steroids in human serum is essential for biomonitoring physiological functions such as hormone regulation, reproduction, and immune response in clinical research laboratories. Liquid chromatography–tandem mass spectrometry (LC–MS/MS) is the gold standard for steroid analysis due to its superior sensitivity, selectivity, and multiplexing capability compared to immunoassays. However, steroid measurements remain challenging because of low endogenous concentrations and complex serum matrices. Here, we describe a robust and sensitive LC–MS/MS method for the simultaneous quantification of 18 steroids in human serum using the Thermo Scientific TSQ Certis triple quadrupole mass spectrometer (Figure 1). The method leverages advanced ion source design and fast acquisition to deliver enhanced sensitivity, stability and throughput suitable for high-volume laboratory workflows.</p><h4><strong>Materials and methods</strong></h4><h5><strong>Liquid chromatograph – mass spectrometry&nbsp;</strong></h5><p>Samples were analyzed on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/744"><strong>Thermo Scientific Vanquish Horizon UHPLC system</strong></a> coupled to the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2207"><strong>TSQ Certis MS</strong></a><strong> </strong>operated in selected reaction monitoring (SRM) mode. The mobile phase composition, analytical column details, LC gradient, and overlaid extracted ion chromatograms (EICs) are shown in Figure 3.&nbsp;</p><h5><strong>Data analysis&nbsp;</strong></h5><p>Data were acquired and processed using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77"><strong>Thermo Scientific TraceFinder software</strong></a> (v 5.2).</p><h4><strong>Conclusions&nbsp;</strong></h4><p>A sensitive and robust LC–MS/MS method was developed for simultaneous quantification of 18 steroids in human serum using the Vanquish Horizon UHPLC and TSQ Certis MS with OptaMax Plus ion source. The method demonstrated excellent linearity, accuracy, and reproducibility across a wide dynamic range. Optimization of MS conditions, particularly vaporizer temperature, significantly improved sensitivity. Overall, the workflow delivers high sensitivity, selectivity, and long-term stability, providing a reliable solution for high-throughput steroid analysis in clinical and research settings.</p><h3><strong>4. Waters Corporation: Alkaline-hydrolysis Method for PFAS in Textiles and Textiles Products by Xevo™ TQ-S micro with ACQUITY™ UPLC™ H-Class Plus System</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009443_en_0ab4914d72.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><h5><strong>Benefits&nbsp;</strong></h5><ul><li>Alkaline hydrolysis method (EN 17681-1:2025) used for preparing textiles, coated textiles and textile products for PFAS analysis allowed the inclusion of side-chain fluorinated polymers&nbsp;</li><li>Extraction method provided significant advantages in handling complex textile matrices by creating clear extracts free from dispersions, settleable solids and particulates&nbsp;</li><li>Protected the high-value LC-MS/MS components from potential damage&nbsp;</li><li>The method improved analytical sensitivity, reproducibility and accuracy, enabled cost-effective and timeefficient PFAS analysis maintained high analytical quality even for challenging high-matrix samples</li></ul><p>Many PFAS are linked to health concerns because of their ability to interfere with the body’s hormone systems, acting as endocrine disruptors also, linked to other toxic effects. Since textiles come into direct and prolonged contact with the skin, there is an increased risk of human exposure—especially for babies and children.4 In addition, PFAS pose significant environmental concerns. These “forever chemicals” do not readily break down and can be released during manufacturing, washing and disposal, allowing them to enter water pathways and persist in the environment. As a result, testing for PFAS has become increasingly important in the textile industry to demonstrate supply chain transparency, build consumer trust and protect brand reputation.</p><p>Synthetic fluorinated chemicals are categorized into two main groups - non-polymeric PFAS and polymeric PFAS, known for their use as water and grease repellent, heat resistant finishes in textiles, upholstery, leather, apparel, carpet and other industrial applications such as packaging, firefighting foams, non-stick cookware, etc. (Figure 1). PFAS are fluorinated substances contain at least one fully fluorinated methyl or methylene carbon atom (without any H/Cl/Br/I atom attached to it), i.e. with a few noted exceptions, any chemical with at least a perfluorinated methyl group or a perfluorinated methylene group.2 PFAS are classified as long chain and short chain based on their perfluorinated carbon chain length. Short chain fluorinated polymers encompasses a diverse range of chemistries, categorized according to their generic chemical and repeating units structures. Few examples are: derivatives of fluorotelomers, perfluoropolyethers with varying carbon chain lengths and acrylates, epoxylates, urethane, silicones of repeated units based structures.3 Polyfluoroalkyl substances such as fluorotelomer alcohols (FTOHs) contain C–H bonds, rendering them more susceptible to degradation. The ethoxylates in fluorotelomers-methacrylate break down to FTOH whereas polymeric fluorinated urethanes, acrylates break down to PFCAs (non-polymeric and polymeric side chain fluorinated polymers). In contrast, perfluoroalkyl substances such as PFOS and PFOA are extremely persistent due to their fully fluorinated carbon chains.&nbsp;</p><p>In alkaline hydrolysis methods, methanolic aqueous sodium hydroxide is used to break down precursor PFAS such as FTOHs, branched side chain PFAS. This process releases terminal perfluorinated carboxylic acids (PFCAs), which can then be measured. However, the efficiency of hydrolysis varies among different PFAS compounds and the presence of interfering substances such as organic matter or other chemicals in the sample matrix can inhibit the cleavage and detection of these analytes.</p><h4><strong>Experimental</strong></h4><ul><li><strong>LC system:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/198"><strong>ACQUITY UPLC H-Class Plus System</strong></a> and SMFTN fitted with PFAS kit</li><li><strong>Column(s):</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1823"><strong>XBridge™ BEH™ C18 5 µm Column</strong></a>, 4.6 x 100 mm&nbsp;</li><li><strong>MS system: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/209"><strong>Xevo TQ-S micro Mass Spectrometer</strong></a></li></ul><h4><strong>Conclusion&nbsp;</strong></h4><p>By strictly following the critical steps of the alkaline hydrolysis method using methanolic aqueous sodium hydroxide (NaOH) both freely extractable PFAS and those previously bound within side-chain fluorinated polymers such as esters linked to fluorotelomer alcohols (n:2 FTOHs, where n = 4, 6, 8, 10, 12), can be effectively extracted. This approach also safeguards the LC-MS/MS instrumentation from blockages, damage, or contamination by preventing polymer accumulation and matrix interferences.&nbsp;</p><p>The analytical method using the ACQUITY UPLC H-Class Plus System and Xevo TQ-S micro LC-MS/MS System was found to be sensitive, reproducible and accurate, enabling cost-effective and time-efficient PFAS analysis in chemically processed textiles, coated textiles and textile products whilst maintaining high analytical quality even for challenging high-matrix samples.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 34, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Palacký University Olomouc (UP) is an internationally respected and scientifically and academically highly productive university in Central Europe. Founded 22 December 1573, it is the second-oldest university in the Czech Republic and the oldest one in Moravia. For 450 years it has contributed to the intellectual wealth of society and to its scientific, cultural, and social development.

Palacký University Olomouc

Phenomenex Luna SCX HPLC Columns

The Luna SCX is a high performance silica phase with a benzene sulfonic acid bonded phase used for Strong Cation Exchange (SCX) chromatography.

<p><strong>The Luna SCX</strong> is a high performance silica phase with a benzene sulfonic acid bonded phase used for <strong>Strong Cation Exchange (SCX) chromatography</strong>.</p><p><strong>Key Features of the SCX Column:</strong></p><ul><li>Extensive line of rugged particle sizes.</li><li>Easy method scalability from HPLC to preparative and bulk purification LC</li><li>Ultra-pure, metal-free silica (99.99% purity)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Luna_SCX_HPLC_Column_4c175db0e0.webp" alt="Phenomenex: Luna SCX HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Luna_SCX_HPLC_Column_4c175db0e0.webp 245w," sizes="100vw" width="500" height="150"></p><ul><li><strong>Carbon Load:</strong> 0.55 % Sulfur Load, null %</li><li><strong>Surface Area (m²/g)</strong>: 400</li><li><strong>Particle Size</strong>: 5, 10</li><li><strong>Recommended Use:</strong> Strong cation exchange for positively charged compounds</li></ul><h4><strong>Long Column Lifetimes and Excellent Performance</strong></h4><p>Ultra-pure, metal-free silica (99.99% purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.</p><h4><strong>Develop Robust Methods</strong></h4><p>Luna SCX columns provide excellent resolution and peak shape of basic‚ cationic compounds. Luna SCX columns contain a benzene sulfonic acid ligand providing ion-exchange‚ reversed phase‚ and aromatic interactions.</p><h4><strong>Common Applications:</strong></h4><ul><li>Cough and cold compounds, Raclopride, Sodium Acetate, Erythromycins</li></ul><blockquote><p class="cke-user_style-important"><strong>Stationary phase Luna SCX is provided in these column types: analytical, midbore and semi-preparative</strong></p></blockquote><h5><strong>Luna SCX HPLC MidBore, Analytical and Semi-preparative Columns are available with these parameters:</strong></h5><p><strong>MidBore Columns</strong></p><ul><li><strong>Column length (mm): </strong>150</li><li><strong>Column ID (mm): </strong>3</li><li><strong>Particle size (µm):</strong> 5</li></ul><p><strong>Analytical Columns</strong></p><ul><li><strong>Column length (mm): </strong>50,<strong> </strong>100, 150, 250</li><li><strong>Column ID (mm): </strong>4.6</li><li><strong>Particle size (µm):</strong> 5</li></ul><p><strong>Semi-Prep Columns</strong></p><ul><li><strong>Column length (mm): </strong>250</li><li><strong>Column ID (mm):</strong> 10</li><li><strong>Particle size (µm):</strong> 5</li></ul><h3><strong>Phenomenex Luna HPLC Columns</strong></h3><p><strong>Luna HPLC columns</strong> are renowned for their outstanding reliability and adaptability in high-performance liquid chromatography (HPLC). Manufactured by <strong>Phenomenex</strong>, Luna HPLC columns are made from <strong>99.999% ultra-pure</strong>, <strong>metal-free silica</strong>, with meticulous attention given to quality control across all aspects of its structure and chemistry. This guarantees that Luna columns consistently deliver reliable performance, ensuring dependable method reproducibility. The Luna series offers diverse selectivity, making it suitable for a broad range of <strong>chromatographic applications</strong>, from <strong>microbore and fast LC</strong> to <strong>preparative</strong> and <strong>bulk purification scales</strong>.</p><p>All Luna products uphold the high standards our customers depend on. Featuring high surface area particles, Luna columns deliver improved resolution and performance, meeting the stringent requirements of HPLC users. Designed for longevity and consistent results, these columns are the trusted choice for both routine analyses and specialized separations, ensuring top-tier chromatographic outcomes.</p><h4><strong>Explore Luna Columns</strong></h4><ul><li><strong>Proven Performance:</strong> Extensive line of rugged USP and Ph.Eur. phases</li><li><strong>Maximum Reproducibility: </strong>Each column has both a certificate of analysis and a certificate of quality assurance</li><li><strong>Ultra-High Purity:</strong> Metal free silica (99.99% purity) &nbsp;ensures a low activity surface and less secondary interactions</li><li><strong>Scalable:</strong> Phases available in 3,5, 10 and 15 µm particle size for scaling from analytical to prep and bulk purification</li></ul><p>Explore resolution with <strong>10 unique Luna selectivities</strong> and find your next selectivity tool. Combined with high surface area the Luna family of columns offers an excellent HPLC performance for a wide range of chromatographic applications.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg" alt="Phenomenex - Phases of Luna Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Phases_of_Luna_Columns_5b3ae06cee.jpg 180w," sizes="100vw" width="565" height="490"></p>

KNAUER Eurokat H Ion Exclusion/Ligand Exchange Columns (USP L17)

The Eurokat H ionic form is especially designed for the analysis of organic acids and even complex mixtures of acids, sugars and alcohols as well as sugar alcohols.

<p class="cke-user_style-important"><strong>Organic acids and alcohols</strong></p><p><strong>The Eurokat H ionic form </strong>is especially designed for the analysis of <strong>organic acids</strong> and even <strong>complex mixtures of acids</strong>, <strong>sugars</strong> and <strong>alcohols</strong> as well as <strong>sugar alcohols</strong>. It is a <strong>cation exchange</strong> material with 8 % cross linkage of the styrenedivinylbenzene copolymer. Best results are acieved with aqueous eluents and small amounts of anorganic acids, recommended eluent 0.005 M sulfuric acid. The usage of up to 10 % organic content in the mobile phase is possible.</p><h5><strong>Properties</strong></h5><ul><li><strong>Sulfonated cross-linked styrenedivinylbenzene copolymer </strong>phase for the determination of organic acids, carbohydrates and alcohols</li><li><strong>H-form, 8 % cross linkage</strong></li><li>Cation exchanger, extremly long lasting lifetime when correctly handled, best results with aqueous eluents and small amounts of anorganic acids, recommended eluent 0,01 N sulfuric acid, usage of up to 10 % organic content in the mobile phase is possible</li></ul><h5><strong>Technical Data</strong></h5><ul><li>Sulfonated cross-linked styrenedivinylbenzene copolymer with 8 % cross linkage in the ionic form H, available in 10 μm particle size, pressure stable up to maximum 100 bar</li></ul><h5><strong>Recommended application areas</strong></h5><ul><li>Eurokat H is especially designed for the analysis of organic acids and even complex mixtures of acids, sugars and alcohols as well as sugar alcohols. Due to a differing separation process we recommend the use of these type of columns only in 8 mm ID or 4 mm ID.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Eurokat_H_Chromatogram_e2c6665a1f.webp" alt="KNAUER: Eurokat H Chromatogram" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Eurokat_H_Chromatogram_e2c6665a1f.webp 245w," sizes="100vw" width="690" height="281"></p><blockquote><p><strong>Tip:</strong> Eurokat columns are characterized by an extremely long lifetime if handled properly. Especially pressure shocks and organic solvent contents of more than 10 % in the mobile phase should be avoided absolutely.</p></blockquote><h3><strong>KNAUER Ion Exclusion and Ligand Exchange Columns</strong></h3><p><strong>Separation of organic acids, carbohydrates, alcohols on sulfonated cross-linked styrenedivinylbenzene copolymer</strong></p><ul><li><strong>Modifications</strong><ul><li>Eurokat H</li><li>Eurokat Ca</li><li>Eurokat Pb</li><li>Eurokat Na</li></ul></li><li><strong>High Temperature Methods:</strong> Up to 90 °C</li><li><strong>Method Development: </strong>Optional up to 10 % organic solvent</li><li><strong>Chemically stable: </strong>pH 1–12</li><li><strong>Sustainability:</strong> Aqueous eluents protect the environment and are cost-effectiv</li><li><strong>User Friendly:</strong> Simple and robust methods for daily analysis. Versatile in its use.</li><li><strong>Simplicity:</strong> Easy purchase via our online store with fast delivery.&nbsp;</li></ul><h4><strong>Characteristics&nbsp;</strong></h4><p>Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca and Pb). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.</p><h4><strong>Choice of chromatographic conditions&nbsp;</strong></h4><p>The Eurokat stationary phase is designed to be applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at temperatures up to 90 °C with no organic solvents. The best separation of sugars can be achieved using between 60 to 90 °C. To extend separation performance, it is possible to connect up to three columns of Eurokat in series. Eurokat columns require no organic solvents and thus are environmentally friendly.&nbsp;</p><h4><strong>Stability&nbsp;</strong></h4><p>Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage compared with silica-based phases which have a limited lifetime at pH extremes, especially in the higher pH range. Most importantly, Eurokat phases show extraordinary column lifetime stability and are not affected by aqueous solvents.&nbsp;</p><h4><strong>Application areas&nbsp;</strong></h4><ul><li>Carbohydrates and organic acids in softdrinks and fruit juices&nbsp;</li><li>Sugar substitutes</li><li>Food preservatives</li><li>Dairy products</li><li>Urine analytic (Uric acid, Hippuric acid)</li><li>Monitoring of fermentation processes</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg" alt="KNAUER: Eurokat - Principle of ligand exchange mechanism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg 245w," sizes="100vw" width="1382" height="570"></p>

KNAUER Eurokat Pb & Ca Ion Exclusion/Ligand Exchange Columns (USP L34 / USP L19)

The ionic forms Ca and Pb are best suited for the analysis of smaller carbohydrates like mono- and disaccharides and sugars up to a degree of polymerization (DP) of 4. 

<p class="cke-user_style-important"><strong>Analysis of mono- and disaccharides</strong></p><p><strong>The ionic forms Ca and Pb</strong> are best suited for the analysis of smaller carbohydrates like <strong>mono- and disaccharides </strong>and <strong>sugars up to a degree of polymerization (DP) of 4</strong>. It is a cation exchange material with 6 % cross linkage of the styrenedivinylbenzene copolymer. Best results are achieved with aqueous eluents, recommended eluent is pure deionized water. The usage of up to 10 % organic content in the mobile phase is possible.</p><h5><strong>Properties</strong></h5><ul><li><strong>Polymer phase for the determination of mono- and disaccharides</strong></li><li><strong>Pb (USP L34) form, 6 % cross linkage</strong></li><li>Cation exchanger, extremely long lasting lifetime when correctly handled, best results with aqueous eluents, recommended eluent is pure deionized water, usage of up to 10 % organic content in the mobile phase is possible</li></ul><h5><strong>Technical Data</strong></h5><ul><li>Sulfonated cross-linked styrenedivinylbenzene copolymer with 6 % cross linkage in the ionic form Pb, available in 10μm particle size, pressure stable up to maximum 100 bar</li></ul><h5><strong>Recommended application areas</strong></h5><ul><li>Especially designed for the analysis of small carbohydrates up to DP 4</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Eurokat_Pb_Ca_Chromatogram_2054f70e49.webp" alt="KNAUER: Eurokat Pb &amp; Ca Chromatogram" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Eurokat_Pb_Ca_Chromatogram_2054f70e49.webp 245w," sizes="100vw" width="690" height="271"></p><blockquote><p><strong>Tip:</strong> Eurokat columns are used at high temperatures. During equilibration it is very important to have an eye on the backpressure and to start with very low flow rates and increase them only slowly and stepwise after the column has adapted to the temperature.</p></blockquote><h3><strong>KNAUER Ion Exclusion and Ligand Exchange Columns</strong></h3><p><strong>Separation of organic acids, carbohydrates, alcohols on sulfonated cross-linked styrenedivinylbenzene copolymer</strong></p><ul><li><strong>Modifications</strong><ul><li>Eurokat H</li><li>Eurokat Ca</li><li>Eurokat Pb</li><li>Eurokat Na</li></ul></li><li><strong>High Temperature Methods:</strong> Up to 90 °C</li><li><strong>Method Development: </strong>Optional up to 10 % organic solvent</li><li><strong>Chemically stable: </strong>pH 1–12</li><li><strong>Sustainability:</strong> Aqueous eluents protect the environment and are cost-effectiv</li><li><strong>User Friendly:</strong> Simple and robust methods for daily analysis. Versatile in its use.</li><li><strong>Simplicity:</strong> Easy purchase via our online store with fast delivery.&nbsp;</li></ul><h4><strong>Characteristics&nbsp;</strong></h4><p>Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca and Pb). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.</p><h4><strong>Choice of chromatographic conditions&nbsp;</strong></h4><p>The Eurokat stationary phase is designed to be applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at temperatures up to 90 °C with no organic solvents. The best separation of sugars can be achieved using between 60 to 90 °C. To extend separation performance, it is possible to connect up to three columns of Eurokat in series. Eurokat columns require no organic solvents and thus are environmentally friendly.&nbsp;</p><h4><strong>Stability&nbsp;</strong></h4><p>Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage compared with silica-based phases which have a limited lifetime at pH extremes, especially in the higher pH range. Most importantly, Eurokat phases show extraordinary column lifetime stability and are not affected by aqueous solvents.&nbsp;</p><h4><strong>Application areas&nbsp;</strong></h4><ul><li>Carbohydrates and organic acids in softdrinks and fruit juices&nbsp;</li><li>Sugar substitutes</li><li>Food preservatives</li><li>Dairy products</li><li>Urine analytic (Uric acid, Hippuric acid)</li><li>Monitoring of fermentation processes</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg" alt="KNAUER: Eurokat - Principle of ligand exchange mechanism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg 245w," sizes="100vw" width="1382" height="570"></p>

KNAUER Eurokat Na Ion Exclusion/Ligand Exchange Columns

The ionic form Na is best suited for the analysis of sugars oligomers up to a degree of polymerization (DP) of 8. 

<p class="cke-user_style-important"><strong>Analysis of sugar oligomers</strong></p><p><strong>The ionic form Na</strong> is best suited for the analysis of <strong>sugars oligomers</strong> up to a <strong>degree of polymerization (DP) of 8</strong>. It is a cation exchange material with <strong>6 % cross linkage of the styrenedivinylbenzene copolymer</strong>. Best results are achieved with aqueous eluents, recommended eluent is pure deionized water. The usage of up to 10 % organic content in the mobile phase is possible.&nbsp;</p><h5><strong>Properties</strong></h5><ul><li><strong>Polymer phase for the determination of sugar oligomers</strong></li><li><strong>Na form, 6 % cross linkage</strong></li><li>Cation exchanger, extremly long lasting lifetime when correctly handled, best results with aqueous eluents, recommended eluent is pure deionized water, usage of up to 10 % organic content in the mobile phase is possible</li></ul><h5><strong>Technical Data</strong></h5><ul><li>Sulfonated cross-linked styrenedivinylbenzene copolymer with 6 % cross linkage in the ionic form Na, available in 10 μm particle size, pressure stable up to maximum 100 bar</li></ul><h5><strong>Recommended application areas</strong></h5><ul><li>Especially designed for the analysis of sugar oligomers, separates carbohydrates up to DP 8</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Eurokat_Na_Chromatogram_7d3d81fa52.webp" alt="KNAUER: Eurokat Na Chromatogram" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Eurokat_Na_Chromatogram_7d3d81fa52.webp 188w," sizes="100vw" width="686" height="568"></p><blockquote><p><strong>Tip:</strong> Eurokat columns are typically used with 100 % aqueous eluents. For storage, please avoid microbial growth by flushing the Eurokat column with 0.45 µm filtered ultrapure water and storing it at 4 °C.</p></blockquote><h3><strong>KNAUER Ion Exclusion and Ligand Exchange Columns</strong></h3><p><strong>Separation of organic acids, carbohydrates, alcohols on sulfonated cross-linked styrenedivinylbenzene copolymer</strong></p><ul><li><strong>Modifications</strong><ul><li>Eurokat H</li><li>Eurokat Ca</li><li>Eurokat Pb</li><li>Eurokat Na</li></ul></li><li><strong>High Temperature Methods:</strong> Up to 90 °C</li><li><strong>Method Development: </strong>Optional up to 10 % organic solvent</li><li><strong>Chemically stable: </strong>pH 1–12</li><li><strong>Sustainability:</strong> Aqueous eluents protect the environment and are cost-effectiv</li><li><strong>User Friendly:</strong> Simple and robust methods for daily analysis. Versatile in its use.</li><li><strong>Simplicity:</strong> Easy purchase via our online store with fast delivery.&nbsp;</li></ul><h4><strong>Characteristics&nbsp;</strong></h4><p>Eurokat high performance polymer phases were especially developed for the separation of organic acids, carbohydrates, alcohols and even complex mixtures of these compounds. Eurokat is a sulfonated cross-linked styrenedivinylbenzene copolymer available in several ionic forms (H, Ca and Pb). This particular cation exchanger is characterized by an outstanding density of functional groups, making it the ideal choice for your ion exclusion, size exclusion and ligand exchange chromatography.</p><h4><strong>Choice of chromatographic conditions&nbsp;</strong></h4><p>The Eurokat stationary phase is designed to be applicable to a wide range of diverse chromatographic conditions. All Eurokat columns can be used at temperatures up to 90 °C with no organic solvents. The best separation of sugars can be achieved using between 60 to 90 °C. To extend separation performance, it is possible to connect up to three columns of Eurokat in series. Eurokat columns require no organic solvents and thus are environmentally friendly.&nbsp;</p><h4><strong>Stability&nbsp;</strong></h4><p>Eurokat polymer columns are extremely stable over the whole pH range. This is one striking advantage compared with silica-based phases which have a limited lifetime at pH extremes, especially in the higher pH range. Most importantly, Eurokat phases show extraordinary column lifetime stability and are not affected by aqueous solvents.&nbsp;</p><h4><strong>Application areas&nbsp;</strong></h4><ul><li>Carbohydrates and organic acids in softdrinks and fruit juices&nbsp;</li><li>Sugar substitutes</li><li>Food preservatives</li><li>Dairy products</li><li>Urine analytic (Uric acid, Hippuric acid)</li><li>Monitoring of fermentation processes</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg" alt="KNAUER: Eurokat - Principle of ligand exchange mechanism" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Principle_of_ligand_exchange_mechanism_a5b7c21c43.jpg 245w," sizes="100vw" width="1382" height="570"></p>

Galochrom Chiral ION-QD HPLC Preparative Columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan.

<p><strong>Chiral ion exchanger GEN-QD is highly efficient in the enantiomer resolution of acidic compounds.</strong></p><p>This product is now available in high quality and at a reasonable price at our company!</p><p>To create our <strong>chiral sorbent</strong>, we use <strong>3µm fully porous spherical silica particles</strong>, which provide greater efficiency than the original product.</p><p>Additionally, our <strong>Galo-ION-QD-200</strong> is recommended for <strong>biological applications</strong> due to its larger pore size, which improves the separation efficiency for large molecules.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QD_HPLC_kolony_ION_QD_selector_10dd9c7ee5.jpg" alt="Galochrom: Galochrom Chiral ION-QD selector."></p><h5><strong>Premium Quality</strong></h5><p>In every manufactured product, we guarantee the highest standards of quality.</p><h5><strong>Cost-effective</strong></h5><p>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.</p><h5><strong>High-performance</strong></h5><p>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.</p><h3><strong>Ordering information</strong></h3><blockquote><p><strong>Stationary phase Chiral ION-QD is provided in these column types: analytical and preparative</strong></p></blockquote><h4><strong>Preparative Columns</strong></h4><ul><li><strong>Dimensions [mm]: 150 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION05-P150</li><li><strong>Price [€]: 9 800</strong></li></ul></li><li><strong>Dimensions [mm]: 250 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION06-P250</li><li><strong>Price [€]: 11 300</strong></li></ul></li></ul><blockquote><h4><strong>Get up to a 20% discount on your first purchase with us.</strong></h4></blockquote><h3><strong>Reach new heights</strong></h3><p>Spend more time on testing and less time on troubleshooting</p><blockquote><h4><strong>How can our unique columns help you succeed?</strong></h4><p>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:</p><ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4><strong>What makes Chiral </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1241"><strong>ION-QN</strong></a><strong> and ION-QD unique?</strong></h4><p><span style="font-size:20px;"><strong>Designed for stability</strong></span></p><p>The manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.</p><p><strong>The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376"></p><p>During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"></p><h3><strong>Achieve your results</strong></h3><p>Increase your productivity with uniform, stable, and reproducible results</p><h4><strong>High-Quality Silica</strong></h4><p>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.</p><p>In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a<br>result, our columns:</p><ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"></p><h4><strong>Elution Order</strong></h4><p>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers.<strong> Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545"></p><p>The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:</p><ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Galochrom Chiral ION-QN HPLC Preparative Columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan. 

<p><strong>Our company is proud to offer the Galo ION-QN chiral ion exchanger, which is capable of efficiently separating enantiomers of acidic compounds.</strong></p><p>This product is now available in high quality and at a reasonable price at our company!</p><p>To create our <strong>chiral sorbent</strong>, we use <strong>3µm fully porous spherical silica particles</strong>, which provide greater efficiency than the original product.</p><p>Additionally, our <strong>Galo-ION-QN-200</strong> is recommended for <strong>biological applications</strong> due to its larger pore size, which improves the separation efficiency for large molecules.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QN_HPLC_kolony_ION_QN_selector_f3789cb2c1.jpg" alt="Galochrom: Galochrom Chiral ION-QN selector."></p><h5><strong>Premium Quality</strong></h5><p>In every manufactured product, we guarantee the highest standards of quality.</p><h5><strong>Cost-effective</strong></h5><p>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.</p><h5><strong>High-performance</strong></h5><p>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.</p><h3><strong>Ordering information</strong></h3><blockquote><p><strong>Stationary phase Chiral ION-QN is provided in these column types: analytical and preparative</strong></p></blockquote><h4><strong>Preparative Columns</strong></h4><ul><li><strong>Dimensions [mm]: 150 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION03-P150</li><li><strong>Price [€]: 9 800</strong></li></ul></li><li><strong>Dimensions [mm]: 250 x 20</strong><ul><li>Porosity: [Å] 120</li><li>Cat. No.: ION04-P250</li><li><strong>Price [€]: 11 300</strong></li></ul></li></ul><blockquote><h4><strong>Get up to a 20% discount on your first purchase with us.</strong></h4></blockquote><h3><strong>Reach new heights</strong></h3><p>Spend more time on testing and less time on troubleshooting</p><blockquote><h4><strong>How can our unique columns help you succeed?</strong></h4><p>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:</p><ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4><strong>What makes Chiral ION-QN and </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1240"><strong>ION-QD</strong></a><strong> unique?</strong></h4><p><span style="font-size:20px;"><strong>Designed for stability</strong></span></p><p>The manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.</p><p><strong>The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376"></p><p>During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"></p><h3><strong>Achieve your results</strong></h3><p>Increase your productivity with uniform, stable, and reproducible results</p><h4><strong>High-Quality Silica</strong></h4><p>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.</p><p>In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a<br>result, our columns:</p><ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"></p><h4><strong>Elution Order</strong></h4><p>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers.<strong> Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545"></p><p>The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:</p><ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Thermo Scientific Hypersil GOLD SAX HPLC Columns

Analyze smaller organic molecules, including nucleotides and organic acids, quickly and efficiently with Thermo Scientific™ Hypersil GOLD™ SAX LC Columns.

<p><strong>Analyze smaller organic molecules, including nucleotides and organic acids, quickly and efficiently with Thermo Scientific™ Hypersil GOLD™ SAX LC Columns. These quaternary amine strong anion-exchange columns are highly stable for aqueous and low pH mobile phases. Based on high purity silica bonded with an ion-exchange ligand, Hypersil GOLD SAX LC Columns provide outstanding peak shape and sensitivity.</strong></p><h4><strong>Specifications</strong></h4><ul><li><strong>Endcapped:</strong> Yes</li><li><strong>Column type:</strong> Ion Exchange</li><li><strong>Max. Pressure: </strong>5800 psi (400 bar)</li><li><strong>Packing Material:</strong> Silica</li><li><strong>Stationary Phase:</strong> Quaternary Amine</li><li><strong>Surface Area:</strong> 220 m²/g</li><li><strong>Temperature:</strong> 60°C</li><li><strong>USP Type: </strong>L14</li><li><strong>Carbon Load</strong>: 2.5%</li><li><strong>Phase:</strong> Ion Exchange</li><li><strong>Pore Size: </strong>175 Å</li><li><strong>pH: </strong>2 to 8</li></ul><h4><strong>About the Hypersil GOLD Family of HPLC Columns</strong></h4><p>Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. <strong>Twelve different column chemistries</strong> are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Columns_41d5984b62.jpg 150w," sizes="100vw" width="747" height="775"></p><ul><li><strong>Excellent peak symmetry</strong><ul><li>Hypersil GOLD columns <strong>produce highly symmetrical peaks</strong>, which facilitate the detection of low-level impurities that elute immediately after the main component. When there is even minor tailing, small impurity peaks can elute on the shoulder of the main peak, complicating the integration process.</li></ul></li><li><strong>Narrow peaks </strong>for outstanding efficiency</li><li><strong>Enhanced sensitivity</strong> and improved resolution</li><li><strong>Exceptional reproducibility </strong>for reliable chromatography, column after column</li><li>Solutions for <strong>high-throughput screening</strong></li><li><strong>1.9, 3, and 5 μm particle sizes </strong>for all your separation needs</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg" alt="Thermo Fisher Scientific: Hypersil GOLD Columns: Peak symmetry" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Hypersil_GOLD_Peak_symmetry_641b946673.jpg 149w," sizes="100vw" width="880" height="925"></p>

Thermo Scientific Acclaim Trinity P2 HPLC Columns

Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific Acclaim Trinity P2 LC Columns.

<p><strong>Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific™ Acclaim™ Trinity P2 LC Columns. The innovative, mixed-mode column technology provides hydrophilic interaction, HILIC, anion-exchange, and cation-exchange functionalities for flexible method development. Use the Acclaim Trinity P2 LC column to complement the Acclaim Trinity P1 LC column, which simultaneously separates pharmaceutical drug substances and their counterions, to provide a total solution for pharmaceutical counterion analysis by HPLC.</strong></p><p>The Acclaim Trinity P2 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical silica particles, coated with charged nanopolymer particles. The unique surface chemistry includes an inner-pore area of the silica bead modified with a covalently bonded organic layer that provides cation-exchange retention. The outer-pore surface is modified with anion-exchange nano-polymer beads. This chemistry ensures spatial separation of the anion-exchange and cation-exchange regions.</p><h5><strong>High Selectivity for Counterion Analysis</strong></h5><ul><li>Low column bleed; compatible with mass spectrometry (MS) and charged aerosol detection (CAD)</li><li>Hydrolytically stable</li><li>High efficiency</li><li>Available in 3.0μm particle size and 2.1 and 3.0mm diameters, in 50 and 100mm lengths</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Max. Pressure:</strong> 6000 psi (415 bar)</li><li><strong>Packing Material:</strong> Spherical, Fully Porous Silica</li><li><strong>Stationary phase: </strong>Spherical, Nanopolymer Hybrid Silica</li><li><strong>Surface Area:</strong> 100 m²/g</li><li><strong>Temperature:</strong> 45°C</li><li><strong>Phase:</strong> HILIC and Ion Exchange</li><li><strong>Pore Size: </strong>300 Å</li><li><strong>pH: </strong>2.5 to 7</li></ul><h4><strong>About the Acclaim Family of HPLC Columns</strong></h4><p>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.</p><ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958"></p>

Thermo Scientific Acclaim Mixed Mode WAX-1 HPLC Columns

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific Acclaim Mixed-Mode WAX-1 LC columns. 

<p><strong>When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific™ Acclaim™ Mixed-Mode WAX-1 LC columns. The silica-based stationary phase features a hydrophobic alkyl chain with an ionizable terminus that provides both hydrophobic reversed-phase and weak anion-exchange properties. This unique chemistry produces a multimode separation mechanism for simultaneous separation of acidic, basic, and neutral molecules. The selectivity is fully adjustable through changes to ionic strength, pH, or organic solvent content.</strong></p><h5><strong>Ideal Selectivity for Separating Anionic Molecules</strong></h5><ul><li>Multi-mode retention mechanism: reversed-phase, weak anion-exchange, and HILIC modes</li><li>Differentiation of molecules with similar hydrophobicities, such as hydrophilic organic acids</li><li>Selectivity orthogonal to reversed-phase columns</li><li>Excellent column efficiency and peak symmetry</li><li>Stable between pH 2.5 and 7.5</li><li>Available in 3 and 5μm particle sizes with an average pore diameter of 120Å</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Column type:</strong> Mixed mode</li><li><strong>Max. Pressure:</strong> 5800 psi (400 bar)</li><li><strong>Packing Material:</strong> Spherical, Fully Porous, Ultrapure Silica</li><li><strong>Stationary phase: </strong>Hydrophobic Alkyl Chain with an Ionizable Terminus</li><li><strong>USP Type:</strong> L78</li><li><strong>Surface Area:</strong> 300 m²/g</li><li><strong>Temperature:</strong> 50°C</li><li><strong>Phase:</strong> Reversed Phase, Ion Exchange, HILIC</li><li><strong>Pore Size: </strong>120 Å</li><li><strong>pH: </strong>2.5 to 7.5</li></ul><h4><strong>About the Acclaim Family of HPLC Columns</strong></h4><p>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.</p><ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958"></p>

Thermo Scientific Acclaim Mixed Mode WCX-1 HPLC Columns

Separate cationic molecules with powerful adjustable selectivity control using Thermo Scientific Acclaim Mixed-Mode WCX-1 LC columns.

<p><strong>Separate cationic molecules with powerful adjustable selectivity control using Thermo Scientific™ Acclaim™ Mixed-Mode WCX-1 LC columns. The silica-based stationary phase features a hydrophobic alkyl chain with a carboxyl terminus that provides both hydrophobic reversed-phase and weak cation-exchange properties. This multimode separation mechanism delivers simultaneous separation of basic and neutral compounds. By independently or simultaneously tuning the mobile phase ionic strength, pH, and organic modifier, you can control the selectivity of ionizable and neutral compounds.</strong></p><h5><strong>Retains and Separates Highly Hydrophilic to Highly Hydrophobic Basic Molecules</strong></h5><ul><li>Multimode retention mechanism: reversed-phase, weak cation-exchange, anion-exclusion, and HILIC modes</li><li>Selectivity complementary to C18 reversed-phase columns</li><li>Efficient primary column for a variety of basic analytes; also an ideal column for secondary methods development</li><li>Versatile in applications that require different selectivities</li><li>Symmetrical peak shapes and excellent efficiency</li><li>Stable between pH 2.5 and 7.5</li><li>Available in 3 and 5μm particle sizes with an average pore diameter of 120Å</li></ul><h4><strong>Specifications</strong></h4><ul><li><strong>Column type:</strong> Mixed mode</li><li><strong>For use with: </strong>RPLC, Cation Exchange, Ion-Exclusion, HILIC</li><li><strong>Max. Pressure:</strong> 6000 psi (415 bar)</li><li><strong>Packing Material:</strong> Silica</li><li><strong>Stationary phase: </strong>Hydrophobic Alkyl Chain with a Carboxyl Terminus</li><li><strong>USP Type:</strong> L85</li><li><strong>Surface Area:</strong> 300 m²/g</li><li><strong>Temperature:</strong> 50°C</li><li><strong>Phase:</strong> Reversed Phase, Ion Exchange, Ion Exclusion, HILIC</li><li><strong>Pore Size: </strong>120 Å</li><li><strong>pH: </strong>2.5 to 7.5</li></ul><h4><strong>About the Acclaim Family of HPLC Columns</strong></h4><p>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.</p><ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958"></p>

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