Learn how to balance purity, yield, and throughput when scaling analytical HPLC methods to preparative chromatography.

Discover how hybrid HPLC materials extend pH stability up to 12, improving retention and peak shape for basic compounds. Learn how Gemini™ and Kinetex™ EVO columns enhance LC-MS/MS performance.

Learn how to minimize peak tailing when analyzing basic compounds on type A silicas. Explore pH adjustment, mobile phase modifiers, and modern type B silica columns for sharper, more symmetrical peaks.

Discover the new Thermo Scientific Vanquish CAD P Series – the next evolution in HPLC detection. Improved sensitivity, method transfer tools, and real-time optimization.

Learn how silica end-capping improves HPLC column performance. Discover how C18, PS-C18, Polar C18, and XB-C18 chemistries enhance retention, peak shape, and selectivity for various analytes.

Discover how bio-inert HPLC hardware enhances oligonucleotide analysis. Learn how BioTi™ columns improve resolution, sensitivity, and reduce adduct formation compared to stainless steel.

Dive deeper into Charged Aerosol Detection (CAD) technology in HPLC. Learn how nebulization, evaporation, charging, and detection steps deliver consistent, mass-based quantification across analytes.

Learn how the choice and concentration of alkylamine ion-pair agents affect oligonucleotide retention and detection in HPLC. Explore method optimization with Phenomenex Biozen™ Oligo columns.

Compare Charged Aerosol Detection (CAD) with ELSD and MALS to see why CAD delivers superior sensitivity, uniform response, and robustness for a broad range of HPLC applications.

Learn how to develop robust HPLC methods for peptide analysis using systematic screening of column chemistry, pH, and gradients for optimal selectivity and reproducibility.