Discover practical tips to optimise LC systems for UHPLC performance on any HPLC. Learn how to reduce peak broadening, improve efficiency, and shorten runtimes.

After SPE, samples may not be ready for direct LC injection. Solvent mismatch with the mobile phase can distort peak shapes—proper solvent choice is essential.

Collision-based ion activation, developed nearly 80 years ago, remains the most widely used fragmentation method. Learn about three key techniques on Thermo Scientific MS.

Practical troubleshooting guide for low recovery in SPE methods. Learn how to identify problems with solvents, flow rate, pH, or sorbent choice step by step.

Column contamination is a leading cause of shortened HPLC column lifetime, leading to high back pressure. Learn why it happens and how to prevent blocked frits with simple precautions.

Over-drying silica-based SPE cartridges during conditioning can reduce analyte retention and recovery. Learn best practices to avoid losses and ensure reproducible results.

Polysaccharide chiral stationary phases enable wide enantiomeric recognition, solvent flexibility, and robust resolution with coated or immobilised designs.

Chiral separations require careful screening of stationary phases. Learn how a systematic approach with polysaccharide-based columns streamlines method development.

Best practices for sample preparation in peptide mapping with trypsin, covering denaturation, alkylation, buffer exchange, and protease selection for complete sequence coverage.

C18 columns with residual positive charge improve peak shape of basic analytes at low pH by reducing silanol interactions and enabling 100% aqueous mobile phases.