<p><strong>He is one of only ten scientists working in Europe who secured the prestigious EMBO Installation Grant this year. After four years in the United States, immunologist Matouš Vobořil recently returned to the Faculty of Science at Charles University to build his own team and advance research focused on the development of immune cells and their training in the thymus. His efforts to combat autoimmune diseases have already caught the attention of numerous grant review panels.</strong></p><h5><strong>Reportedly, one of the motivations for your studies was the awareness that in science, there is still more that is unknown than what is known. You made this statement after receiving the Doctorandus Award for Natural Sciences in 2020, adding that during your lifetime you would like to shift something toward the “known” category. How has that been going so far?</strong></h5><p>The topic for which I&nbsp;take the greatest credit to date is one I&nbsp;began exploring during my doctoral studies and fully developed as a&nbsp;postdoctoral fellow in the U.S., and that is inflammation of the thymus. When you delve into the literature and articles focused on autoimmune diseases, eventually you begin to notice that autoimmune diseases often develop after undergoing some kind of infection. This is particularly evident in connection with long COVID. During inflammatory processes in the body, the immune system responds, and we were interested in whether there is a&nbsp;mechanism that recognises this inflammatory response and teaches T-lymphocytes (immune cells) that this is not a&nbsp;problem, but rather a&nbsp;process inherent to the body.</p><p>During my doctoral studies, I&nbsp;investigated the basic inflammatory response in the thymus, without the presence of actual bacterial or viral inflammation. This turned out to be extremely important. Over time, we discovered that the thymus contains many antiviral molecules and molecules that play a&nbsp;key role in allergic reactions or in the recognition of bacteria and viruses. These molecules create a&nbsp;specific environment in the thymus that mimics what may happen in our bodies later in life, and they train T-cells not to respond to these stimuli. I&nbsp;consider this to be my greatest contribution to the field. Of course, there are still many unexplored areas in this field.</p><h5><strong>The interview I was referring to was published in the magazine of the grammar school where you studied, and which proudly acknowledges your scientific achievements. Was that where your interest in science first began to show?</strong></h5><p>The fact that I&nbsp;studied in Dvůr Králové nad Labem was a&nbsp;huge stroke of luck. Not only is there a&nbsp;zoo, where my parents used to work, so I&nbsp;spent a&nbsp;lot of time there, but thanks to that, I&nbsp;also joined the local Natura club, which brought together people interested in nature and was led by the Dobroruk couple. When I&nbsp;was ten, my parents sent me to a&nbsp;nature-oriented summer camp with them, and I&nbsp;ended up staying with them – which is why I&nbsp;also attended the local high school, where Mrs. Dobroruková taught. She was a&nbsp;wonderful teacher. Her classes really encouraged students to think about what’s&nbsp;happening in nature. Thanks to that, I&nbsp;began participating in biology competitions and got involved in the Czech high school research competition. Although my first scientific experiments focused on ornithology, they gave me the opportunity to present scientific data and meet many people from the Faculty of Science at Charles University, including Professor Jan Černý, who lectured our class on immunology. Suddenly, while still in high school, I&nbsp;realized that this was the path I&nbsp;wanted to take.</p><h5><strong>During your master’s studies, you joined the team of immunologist Dominik Filipp at the Institute of Molecular Genetics of the Czech Academy of Sciences, where you then continued as a doctoral student. How did working with him influence you, and where did it lead you?</strong></h5><p>Dominik Filipp had several ongoing projects related to the peripheral immune response, signalling, and identification of viruses and bacteria. The project I worked on focused on a rare population of cells found in lymph nodes or the spleen. I studied the effect of pathogen-recognising receptors on these cells. Since these cells are similar to those in the thymus and I needed a negative control, I used the thymus for that purpose. To our surprise, the result was not negative but positive. This led us to the phenomenon itself and prompted us to begin investigating its consequences in the thymus.</p><h5><strong>You then travelled overseas to study this discovery – which you actually made rather by chance – in detail. What was your experience like at American research institutions?</strong></h5><p>Amazing. I&nbsp;went to the University of Minnesota to work with particular people, especially my future supervisor, Kristin Hogquist. I&nbsp;had met her at several conferences while I&nbsp;was still in my PhD programme, and I&nbsp;had a&nbsp;feeling that our collaboration could work well both professionally and personally.</p><p>But we were also fortunate to pick Minnesota. It’s&nbsp;a&nbsp;great place to live, and at the same time, excellent science is being conducted there. The Center for Immunology, where I&nbsp;worked, rivals the world’s&nbsp;leading scientific institutions in terms of its research results. I&nbsp;think this is mainly down to the highly supportive environment there, which fosters collaboration. Thanks to this, you can really grow as a&nbsp;scientist there.</p><p>In addition, they focus on explaining and describing the chosen phenomenon as a&nbsp;whole, rather than worrying too much about whether it will result in one or five articles, or whether it will take ten years. Their method of funding supports this approach. In a&nbsp;system of short-term grants, as we know it here, you can’t structure research this way. That’s&nbsp;a&nbsp;major disadvantage for us.</p><h5><strong>How did you develop your own research there?</strong></h5><p>It was there that I&nbsp;fully realized the potential of what we had discovered in the lab in Prague. Kristin Hogquist and her team were working on something similar, but it wasn’t until we teamed up that we were able to take it further. I&nbsp;focused primarily on how various antiviral signals or signals triggered during allergic reactions influence T-cell training and defence against autoimmune diseases following viral infections or allergic reactions. Suddenly, it became clear that this was a&nbsp;vast topic and the foundation for establishing a&nbsp;separate subfield of research on tolerance (the training of immune cells in the thymus so that they do not harm the body while remaining capable of fighting pathogens).</p><p>One great thing was that two labs were coming together at the facility: the one led by Kristin Hogquist and the other led by her husband, Stephen Jameson, which focuses on how different types of infections affect T-cell activation and their response. I&nbsp;realised that these two areas needed to be connected, and I&nbsp;based my own research plan on that – namely, to study how the inflammatory environment, actual inflammation, and viral or bacterial infections can retroactively influence processes in the thymus. This is a&nbsp;topic that no one has addressed yet.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_2_b020119f2e.jpg" alt="Forum UK/Petr Jan Juračka: Vobořil: There is still much to learn about the immune system: As head of research, Matouš Vobořil strives to ensure that everyone on his team feels comfortable." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_2_b020119f2e.jpg 234w," sizes="100vw" width="1000" height="667"></p><h5><strong>You mentioned how important it was to you that you got along with Kristin Hogquist not only professionally but also on a&nbsp;personal level. To what extent did you take this “personal” aspect into account when putting together your own team at CU SCI?</strong></h5><p>This is tremendously important to me. Kristin Hogquist is not only an outstanding researcher, but also a&nbsp;very kind person. And that really helped me feel at home in the American environment, which sometimes tends to become highly competitive. I&nbsp;realized that I&nbsp;would like those who will one day work on my own team to feel the same way I&nbsp;did on hers.</p><h5><strong>So far, we’ve been talking about what led you to science. Have you, on the other hand, encountered anything during your career so far that has discouraged you from pursuing science?</strong></h5><p>Moving overseas with my family and my two-and-a-half-year-old son during the pandemic wasn’t the easiest thing to do. We had to apply for a&nbsp;national exemption just so that I&nbsp;and my family would be allowed into the U.S. at all. Fortunately, as an immunologist, I&nbsp;fell into a&nbsp;special category subject to less stringent pandemic restrictions. Packing up the family, moving overseas, and learning to navigate a&nbsp;completely different system is undoubtedly a&nbsp;major challenge. It required sacrifices not only from me, but above all from my wife, who had to put her own career on hold for several years because of mine. This is one of the biggest and most demanding obstacles that scientists and their families must overcome.</p><p>The second major obstacle you have to deal with as a&nbsp;scientist is paperwork – something I’ve only fully realized now that I’m leading a&nbsp;research group. Even though you hear about it from those around you, it still doesn’t prepare you for when it comes. As a&nbsp;trainee, you do science and conduct experiments in the lab, but suddenly everything shifts, and you become a&nbsp;manager and administrator of all sorts of things. I&nbsp;spend about 30 percent of my time on paperwork. It’s&nbsp;not exactly fun.</p><h5><strong>You obtained a&nbsp;Primus grant, a&nbsp;Junior Star grant from the Czech Science Foundation, and most recently an EMBO Installation Grant. What research challenges do you now face with these grants?</strong></h5><p>I&nbsp;would also like to apply for an ERC Starting Grant this year as well. In the Junior Star project, we are studying cell migration to the thymus. We hypothesize that so-called dendritic cells migrate to the thymus from the periphery (skin, lungs, intestines) – that is, from sites exposed to the external environment and inflammation. These cells are thought to bring information about their own antigens to the thymus and teach developing T-cells tolerance. We aim to determine whether this migration actually occurs, under what circumstances, and how important it is for establishing tolerance to specific tissues. This could help answer the question of why autoimmune skin diseases, such as psoriasis, develop following inflammatory processes.</p><p>The second objective is broader. We would like to answer a&nbsp;major question in immunology: <strong>why do autoimmune diseases develop after a&nbsp;person has had an infection? </strong>We want to study how different types of inflammation (viral, bacterial, or yeast infections) affect the training of immune cells in the thymus.</p><p>We would like to introduce a&nbsp;system of so-called “dirty mice.” The entire field of immunological research is based on working with mice raised in sterile conditions so that we can easily manipulate them and observe how these manipulations affect the immune system. In reality, however, the immune system does not function this way. I&nbsp;brought this idea back from the University of Minnesota, where they placed standardized mice in the same cage with mice purchased from various pet stores around Minneapolis. After a&nbsp;few weeks to months, the mice contract almost everything the store-bought mice have, thereby developing a&nbsp;true physiological immune response. Yet they are still the same mice – genetically identical – that we can manipulate. This is an excellent system that allows us to study immune responses in a&nbsp;much more physiological context.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_3_c458457e12.jpg" alt="Forum UK/Petr Jan Juračka: Vobořil: There is still much to learn about the immune system " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Forum_UK_Voboril_O_imunitnim_systemu_toho_stale_mnoho_nevime_3_c458457e12.jpg 234w," sizes="100vw" width="1000" height="667"></p><h5><strong>Will you be applying for a&nbsp;European Research Council (ERC) grant with that goal in mind?</strong></h5><p>Exactly.</p><h5><strong>In order to succeed in the competition for ERC grants, the projects should push the boundaries of knowledge, which also involves a&nbsp;certain degree of risk. Where do you see the greatest risks in your own research?</strong></h5><p>There are many risks. One of the biggest is that although all the infections we plan to introduce are controlled and well-documented, you still never have full control over them. So it’s&nbsp;possible that not only will the mice become peripherally infected, but the thymus itself will also become infected, which could complicate the experiments. At the same time, this is a&nbsp;physiological system – and I’m talking mainly about the “dirty mice” – which means that each mouse will respond a&nbsp;little differently, and that will generate enormous variability in the samples. Immunologists strive to have the purest system possible, so that when they introduce, for example, a&nbsp;single infectious agent, they can then observe exactly what’s&nbsp;happening there. In this regard, we are actually taking a&nbsp;step back, so to speak, because we want it to be more variable and closer to reality. Setting up and analysing data from such experiments so that they’re reproducible and reveal a&nbsp;real phenomenon can be a&nbsp;real challenge. But that’s&nbsp;the only way we can get closer to real life.</p><h5><strong>The most recent Nobel Prize in Physiology or Medicine was awarded to three immunologists: Mary E. Brunkow, Fred Ramsdell, and Shimon Sakaguchi, who recently gave a&nbsp;lecture in Prague. Did you meet him? How much did awarding the Nobel Prize to immunologists motivate you further in your work?</strong></h5><p>When Shimon Sakaguchi was in Prague, I&nbsp;was still in the U.S., but I&nbsp;did meet him several times at various conferences and even had the opportunity to talk with him on multiple occasions, given that we’re both involved in T-cell research. In our community, people have long been placing bets on when they’ll finally get the Nobel Prize. For me, their discoveries are truly inspiring, but the Nobel Prize itself doesn’t motivate me that much anymore. I&nbsp;find the story behind it to be more interesting. It took a&nbsp;very long time for the scientific community to accept their findings. One of the seminal articles was published in the <i>Journal of Immunology</i>, which isn’t a&nbsp;journal with as high a&nbsp;profile as <i>Nature</i> or <i>Science,</i> and not one where you’d expect to read about such a&nbsp;groundbreaking discovery. It took several years before it became clear just how significant this discovery was. Now I&nbsp;often remind my students of this – even an article in the <i>Journal of Immunology</i> can win you a&nbsp;Nobel Prize, and that is incredibly inspiring.</p><blockquote><h5><strong>Matouš Vobořil, PhD</strong></h5><p>Graduate of the Faculty of Science at Charles University. His research focuses on the development of immune cells in the thymus, the onset of autoimmune diseases, and the function of antigen-presenting cells. After spending four years as a&nbsp;postdoctoral fellow at the University of Minnesota on Kristin Hogquist’s&nbsp;team, he returned to Europe in 2025. Thanks to a&nbsp;Primus grant from Charles University, he established his own research group at the Faculty of Science. He subsequently received a&nbsp;Junior Star grant from the Czech Science Foundation and, this January, an EMBO Installation Grant. For his dissertation, he was awarded the Doctorandus Prize as part of the Česká hlava initiative.</p></blockquote>

Vobořil: There is still much to learn about the immune system 

<h3><strong>How are PFAS regulations changing, and what role will ion chromatography play in the future of environmental testing?</strong></h3><p>In this episode, &nbsp;David interviews <strong>Jay Gandhi, PhD, </strong>Vertical Markets Manager at <strong>Metrohm USA</strong>, for an in-depth discussion on PFAS analysis, the rise of AOF (Adsorbable Organic Fluorine) testing, and the development of EPA 1621 and ISO 18127 standards.</p><p>With more than 30 years of experience in ion chromatography, Jay shares valuable perspective on how the field has evolved—from early applications and NASA water recovery projects to today’s challenges involving PFAS, TFA, and emerging contaminants.</p><h4><strong>Topics covered in this episode include:</strong></h4><ul><li>What PFAS are and why they matter</li><li>The difference between targeted PFAS methods and AOF analysis</li><li>How combustion ion chromatography works</li><li>Behind the scenes of EPA 1621 and ISO 18127 method development</li><li>Regulatory trends in water, textiles, and food packaging</li><li>TFA analysis using IC-MS and IC-MS/MS</li><li>The future of ion chromatography in environmental labs</li></ul><h3><strong>Video Transcription</strong></h3><p>For more than three decades, Jay Gandhi has worked with ion chromatography (IC), watching the technique expand from a specialized tool for water analysis into an analytical platform used across environmental testing, food and beverage analysis, semiconductor manufacturing, battery applications, and, increasingly, PFAS analysis.</p><p>In an interview for <i>Concentrating on Chromatography</i>, Gandhi discusses his introduction to IC during his work on a NASA Mars exploration project, the emergence of PFAS as a major analytical challenge, and why techniques such as adsorbable organic fluorine (AOF) determination and combustion ion chromatography (CIC) can provide information that targeted LC-MS analysis alone cannot deliver. He also examines the development of standardized methods, sample preparation challenges, and the future role of IC-MS and total fluorine approaches in PFAS monitoring.</p><h4><strong>From NASA to ion chromatography</strong></h4><p>Gandhi's introduction to ion chromatography came through his work as a principal scientist on a NASA Mars exploration project focused on water recovery under zero-gravity conditions. IC played an important role because it enabled researchers to establish the ionic balance of anions and cations in water.</p><p>The technology was used both to investigate micronutrients relevant to agricultural applications and to evaluate water purity for potential astronaut consumption. During approximately three years of this work, Gandhi gained extensive experience with ion chromatography. This ultimately led him deeper into IC instrumentation and, 24 years ago, to joining Metrohm.</p><p>At the beginning of his career, IC was strongly associated with relatively straightforward anion and cation analysis in water. The technique itself had been used for water analysis since the 1970s, but Gandhi has seen its application range expand dramatically.</p><p>Food and beverage testing and environmental analysis became important fields, supported by both direct and indirect detection strategies. More recently, IC has also found applications in areas such as the semiconductor industry and battery recycling.</p><p>In Gandhi's words, ion chromatography has effectively "bloomed" into numerous application areas.</p><h4><strong>PFAS changes the analytical challenge</strong></h4><p>PFAS represent a particularly important chapter in this development. These chemicals have been used for decades in numerous consumer and industrial products, including non-stick materials, firefighting foams, water-resistant textiles and other applications.</p><p>Gandhi recalls work on PFOA and PFOS using ion chromatography with conductivity detection as early as 2008. However, the achievable detection limits were insufficient for the extremely low concentrations that later became important in PFAS analysis.</p><p>A major turning point for him came in 2015, when he encountered research from Sweden using <strong>combustion ion chromatography to investigate total fluorine</strong>. Subsequent collaboration with researchers at Clarkson University and Harvard University, particularly on aqueous film-forming foam (AFFF), further demonstrated the importance of looking beyond individual targeted PFAS compounds.</p><h4><strong>Why targeted LC-MS does not tell the whole story</strong></h4><p>Targeted LC-MS is highly sensitive and selective and can provide detailed information about individual PFAS compounds. However, according to Gandhi, the number of possible PFAS structures in the environment is vastly greater than the number routinely included in targeted methods.</p><p>A targeted method may monitor dozens or perhaps around one hundred compounds, while the overall PFAS universe can include thousands of chemical structures. Consequently, targeted analysis may characterize only a small fraction of the total PFAS burden.</p><p>There are other practical considerations. LC-MS measurements can be affected by matrix-dependent ion suppression, while accurate quantification frequently relies on isotope-labelled internal standards. Such standards are available for only a limited number of compounds and can be expensive.</p><p>This is where measurements of total or adsorbable organic fluorine can provide complementary information. Rather than asking <i>which specific PFAS compounds are present?</i>, these approaches can help answer a broader question: <i>how much fluorine associated with this chemical space is present in the sample?</i></p><h4><strong>AOF as a complementary approach</strong></h4><p>Adsorbable organic fluorine analysis provides a different perspective from targeted LC-MS. In the workflow discussed by Gandhi, organic fluorine compounds are captured during sample preparation and subsequently measured using combustion ion chromatography.</p><p>The principle of CIC builds upon older total organic halogen analysis. The sample is combusted at temperatures above approximately 1,000 °C, breaking carbon-halogen bonds. In PFAS-related analysis, carbon-fluorine bonds are broken and the resulting fluoride is collected in an absorption solution.</p><p>Ion chromatography is then used to separate and quantify the fluoride.</p><p>Replacing older detection approaches with IC provides both sensitivity and selectivity for the halogens being measured. Effective combustion is critical: oxygen supports the combustion process, while a carrier gas transports the products into the absorption solution for subsequent IC analysis.</p><h4><strong>The challenge of inorganic fluoride</strong></h4><p>The broad response of CIC is simultaneously one of its strengths and one of its analytical challenges. Combustion does not distinguish fluorine originating from PFAS from fluorine present in inorganic compounds such as fluoride salts.</p><p>This becomes particularly important when analyzing water containing relatively high concentrations of inorganic fluoride while attempting to quantify organic fluorine at very low levels.</p><p>Sample preparation therefore has a critical role. Inorganic fluoride must be effectively separated from the fraction of interest, while materials used during sample preparation must themselves have sufficiently low fluoride backgrounds.</p><p>Contamination can come from unexpected sources. Gandhi points out that background fluoride may originate not only from reagents and sample preparation materials but potentially from the laboratory environment. Careful blank control, appropriate materials and rigorous evaluation of interferences are therefore essential when moving toward increasingly low detection limits.</p><h4><strong>From research method to international standardization</strong></h4><p>The development of standardized AOF methods illustrates the importance of collaboration between academia, industry, regulatory organizations and analytical laboratories.</p><p>Gandhi describes work that began in Europe around 2015 and subsequently progressed through German standardization activities, ISO development, ASTM-related collaboration and the U.S. EPA. The process eventually contributed to methods such as <strong>EPA Method 1621</strong>.</p><p>He emphasizes that this was not the work of a single scientist or organization. Developing a robust method required years of experiments, discussions and sometimes difficult technical debates among numerous stakeholders.</p><p>For Gandhi, the essential principle was that experimental evidence ultimately had to determine the outcome. Collaboration becomes possible when scientists focus on the data rather than the organizations they represent.</p><h4><strong>PFAS monitoring is moving beyond drinking water</strong></h4><p>Regulatory attention to PFAS is also expanding beyond drinking water. Gandhi describes a broader strategy that begins with reducing the manufacture and use of PFAS-containing materials and continues with removing existing contamination from the environment.</p><p>Wastewater represents an important part of this picture because PFAS can enter it from numerous sources. Historical contamination from landfills and contaminated sites can also migrate into groundwater and ultimately affect drinking-water supplies.</p><p>Consumer products are another area of increasing attention. Food packaging, disposable containers, automotive fabrics, outdoor clothing and water-repellent textiles have historically used fluorinated substances to provide properties such as grease, water or stain resistance.</p><p>Reducing PFAS use in such products could therefore gradually decrease the amount entering waste streams and the wider environment.</p><h4><strong>Screening first, regulation later?</strong></h4><p>Gandhi expects AOF to be particularly valuable as a <strong>screening tool</strong>, providing an estimate of broader fluorine contamination before laboratories proceed to more specific analyses.</p><p>Its importance becomes clear when AOF results are compared with targeted PFAS measurements. Gandhi reports that laboratories working with EPA Method 1621 have observed differences between targeted PFAS results and broader AOF measurements ranging from approximately <strong>300- to 4,000-fold in the total fluorine mass balance</strong>.</p><p>Such discrepancies suggest that targeted methods can leave a substantial portion of fluorine unexplained.</p><p>Rather than competing with LC-MS, AOF and CIC therefore provide complementary information. A broad screening method can reveal the magnitude of total contamination, while targeted mass spectrometry identifies and quantifies specific compounds of interest.</p><h4><strong>Where IC-MS fits into the PFAS toolbox</strong></h4><p>No single technique can cover the entire PFAS problem. Gandhi highlights very short-chain, highly polar fluorinated compounds as another analytical challenge.</p><p>Compounds such as trifluoroacetic acid (TFA) are highly water-soluble and may not be quantitatively retained by the carbon materials used for AOF sample preparation. Direct ion chromatography can provide an alternative route for separating such compounds from common inorganic anions.</p><p>Coupling IC with mass spectrometry adds another level of sensitivity and selectivity. Gandhi therefore sees <strong>IC-MS and IC-MS/MS</strong> as increasingly important tools for highly polar fluorinated compounds. Similar approaches can also be useful beyond PFAS, including the determination of polar pesticides such as glyphosate and glufosinate.</p><h4><strong>Toward lower detection limits and more complete PFAS analysis</strong></h4><p>For Gandhi, current standardized methods represent a beginning rather than an endpoint. Improvements in instrumentation and particularly sample preparation could push total fluorine analysis to substantially lower concentrations.</p><p>He notes that current combustion IC configurations can reach approximately <strong>0.5 ppb</strong>, or 500 parts per trillion, and believes that improvements in sample volume, preparation procedures and mitigation of inorganic fluoride backgrounds could eventually move detection below 100 ppt and potentially toward 50 ppt.</p><p>Extractable organic fluorine workflows could provide another bridge between broad fluorine screening and targeted LC-MS methods. Combining improved extraction with CIC could allow samples to be screened for organic fluorine before more detailed compound-specific characterization.</p><p>The broader message from Gandhi's career is that PFAS analysis is unlikely to be solved by a single analytical technique. <strong>Targeted LC-MS provides compound-specific sensitivity and selectivity; AOF and combustion IC reveal a broader fluorine picture; and IC-MS can address highly polar compounds that are difficult to capture using conventional workflows.</strong> Used together, these techniques can provide laboratories with a much more complete understanding of PFAS contamination than any one approach alone.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

The Future of Ion Chromatography | PFAS Testing, EPA 1621 & AOF Explained

<p><strong>In late February 2025, a train carrying benzene derailed in Hustopeče nad Bečvou, and within hours, a race was on to save the groundwater and surface water. One of the most toxic industrial substances began seeping into the gravel-sand subsoil. While fieldwork was underway to prevent the spread of contamination, decisions were already being made in the CXI TUL laboratories regarding how to subsequently clean up the contaminated rock environment.</strong></p><p>The accident ranks among the <strong>most serious environmental incidents in the Czech Republic in recent years</strong>. After a tanker train derailed, hundreds of<strong> metric tons of benzene leaked</strong>; due to the highly permeable geological subsoil, it quickly seeped into deeper soil layers and into the groundwater. The combination of the substance’s high toxicity and local conditions complicated both the emergency response and the subsequent remediation. The key was to stop further spread, determine the extent of the contamination, and establish a remediation strategy for the entire site.</p><p>It was at this stage that CXI TUL became involved in the solution. At the request of the renowned remediation company DEKONTA, a.s., a team led by <strong>RNDr. Jan Němeček, Ph.D.</strong>, began testing the feasibility of the<strong> in situ chemical oxidation (ISCO) method</strong>. This technology is designed to<strong> break down benzene directly within the rock matrix</strong>, without the need for extensive excavation of contaminated soil or the pumping out of large volumes of groundwater. The goal was simple: to find a method that would work quickly, safely, and under the actual conditions of the affected site.</p><h4><strong>Benzene Remediation: Technically Simple, Complicated in Practice</strong></h4><p>Benzene is a highly toxic and carcinogenic substance that rapidly penetrates into groundwater in permeable subsoil, where it dissolves and spreads further as the water flows toward surface water bodies. Benzene also binds to rock particles and migrates in the form of vapors into overlying unconsolidated layers.</p><p>Scientists in Liberec therefore tested <i>in situ </i>chemical oxidation (ISCO) technology under laboratory conditions. The goal was to find an oxidant capable of breaking down benzene directly underground into less hazardous substances.</p><p>The tests evaluated two main options:<strong> activated hydrogen peroxide and sodium peroxodisulfate</strong>. Both substances removed up to<strong> 99.99% of the benzene</strong>, but there was a difference in their kinetics and impacts on the natural environment. Activated hydrogen peroxide achieved nearly complete degradation of the contaminant within a single day. Activated sodium peroxodisulfate was slower and also significantly altered the chemistry of the water; it acidified the environment and increased the sulfate content in the water, which poses an additional environmental burden.</p><p>For field applications, the researchers therefore unequivocally recommended <strong>activated hydrogen peroxide</strong>.</p><h4><strong>Dosage Matters</strong></h4><p>It was not just the oxidant’s effectiveness that was key. It was equally important to determine how the chemical reaction would behave in a real geological environment. The team of scientists therefore also analyzed what is known as <strong>soil oxidant consumption (SOD)</strong>. It turned out that a significant portion of the oxidizing agent does not react solely with benzene but is also consumed to a significant extent by the rock environment itself, particularly by natural organic substances present in the fine-grained fraction of the soil.</p><p>An oxidant consumption value was determined specifically for the local composition of the aquifers, which is essential for the correct dosing of chemicals in the field so that the remediation is not only effective but also economically viable and safe for surrounding ecosystems.</p><h4><strong>One Year After the Accident: Research Turns into Real-World Assistance</strong></h4><p>While the public often perceives university science as a distant world of laboratories and grant projects, it is precisely situations like this that demonstrate its practical impact. The results of the work by researchers at CXI TUL did not remain confined to academic studies but directly influenced decisions regarding the technology used to clean up one of the largest environmental accidents in recent years.</p>

Science on the Front Lines: Cleanup of a Benzene Spill

Article

Video

Scientists

Environment

Interview

The Institute for Nanomaterials, Advanced Technologies and Innovations of the Technical University of Liberec, also known as CXI TUL, was founded in 2009. Our mission is to support TUL's long-term vision of collaboration with industry and innovation.

CXI TUL

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Reportáže, rozhovory, zprávy o vědeckých projektech a studentských akcích ... Jsme online informační bránou Univerzity Karlovy.

Forum: magazín Univerzity Karlovy

Waters Alliance HPLC System

 With its proven performance history, the Alliance HPLC is the industry’s analytical HPLC system of choice, ideally suited for methods development and routine analysis.

<div><div><h4><strong>Reliably achieve quality, compliant data. Every time, in every lab.</strong></h4></div></div><div><div><p><strong>Liquid chromatography (LC) systems must deliver unequivocal results that give you confidence in your scientific and business decisions. Waters LC commitment continues to succeed with the latest Alliance HPLC System – trusted by scientists worldwide for the data to drive informed decisions.</strong></p><div><div><div><div><p>Building on <strong>industry-standard performance and reliability</strong>, the <strong>Alliance HPLC System</strong> provides the <strong>capabilities to meet today’s business challenges</strong>. With its <strong>proven performance history</strong>, the Alliance HPLC is the <strong>industry’s analytical HPLC system of choice</strong>, ideally suited for <strong>methods development and routine analysis</strong>.</p><ul><li>Time-tested, dependable, and robust workhorse for your HPLC separations</li><li>Integrated solvent and sample management with consistent system-to-system performance and high reproducibility</li><li>Simplify your daily LC system start-up with advanced software functionality</li></ul><h5><strong>Recommended Use</strong></h5><p>For standard HPLC workflows where <strong>consistency and compliance</strong> are the highest priorities.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_1_6b63ab6d62/Waters-Alliance-HPLC-System-1.jpg" alt="Waters Alliance HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_1_6b63ab6d62/thumbnail_Waters-Alliance-HPLC-System-1.jpg 147w," sizes="100vw" width="908" height="961"></p><div><div><h3><strong>Excellence through the years</strong></h3><p>The consistency of the Alliance HPLC System gives you <strong>exceptional confidence in your analytical results</strong>, day-to-day and year-to-year. Get <strong>accurate and reproducible results</strong> with <strong>Waters flagship HPLC technology</strong>, which provides <strong>consistent and reliable performance</strong>. Whether your Alliance HPLC System is newly manufactured, or you're working on one that is several years old, the Alliance HPLC System is a <strong>time-tested, dependable,</strong> and <strong>robust </strong>workhorse for your HPLC separations.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_dfafc334c1.jpg" alt="Waters Alliance HPLC System: Comparison of the separation of carbamates using two different Alliance HPLC Systems, manufactured 7 years apart. The results are equivalent." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_dfafc334c1.jpg 245w," sizes="100vw" width="1024" height="532"></p></div></div><div><div><h3><strong>Performance that exceeds your method requirements</strong></h3><p>The <strong>Alliance HPLC System</strong> ensures the <strong>reliability of your results</strong> via <strong>high injection precision</strong> and <strong>repeatable and accurate flow rates</strong>, resulting in <strong>easy peak identification</strong>, <strong>precise quantification</strong>, and <strong>exceptional retention time repeatability</strong>.</p><p>The integrated solvent delivery system features <strong>quaternary blending with a fixed delay volume</strong> for <strong>consistent and predictable results</strong>, regardless of system back pressure. The sample managers’ <strong>flexible injection capabilities</strong> cover a wide injection volume range (up to 2 mL) with <strong>excellent carryover performance</strong>, typically <strong>below 0.005%</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_2_6dab43c365.jpg" alt="Waters Alliance HPLC System: USP method for Budesonide on the Alliance HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_2_6dab43c365.jpg 245w," sizes="100vw" width="1024" height="580"></p></div></div><div><div><h3><strong>Advancements in column chemistry and mass detection</strong></h3><p>The <strong>Alliance HPLC</strong> can leverage <strong>smaller particle size column chemistry</strong> to improve your laboratory's efficiency. With <strong>CORTECS 2.7 µm particle size columns</strong>, your lab will benefit from <strong>improvements in resolution</strong> and <strong>faster run times</strong> when transferring from a 5 µm size particle chemistry.</p><p>Further <strong>increase confidence in your data</strong> by adding <strong>mass confirmation with the ACQUITY QDa Detector</strong>, which is <strong>simple to use</strong> and can easily integrate into your existing workflow. <strong>Analysis in Empower 3 Software</strong> combines data from <strong>UV/Vis and mass spectrometry (MS)</strong> peaks to give <strong>mass information to each peak</strong> in the separation.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_3_3711671b2e.jpg" alt="Waters Alliance HPLC System: Mass detection with the ACQUITY QDa Detector with UV detection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_3_3711671b2e.jpg 245w," sizes="100vw" width="1024" height="550"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_4_bf40981882.jpg" alt="Waters Alliance HPLC System: Mass detection with the ACQUITY QDa Detector with UV detection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_4_bf40981882.jpg 240w," sizes="100vw" width="1024" height="666"></p></div></div><div><div><h3><strong>Features and benefits</strong></h3><ul><li><strong>Quaternary solvent blending. </strong>Support isocratic and gradient applications with 11 unique gradient profile curves and blend up to 4 solvents in any proportion with Auto-Blend Technology. Compatible with solvent types such as reversed-phase, normal-phase, GPC, SEC, and IEX.</li><li><strong>Advanced solvent delivery capabilities. </strong>Serial flow path with pulse-free solvent delivery without the use of dampeners. Online solvent degassing and automated solvent compressibility compensation for accurate flow. Integrated seal wash for robust operation with all solvent types is standard.</li><li><strong>Optional, temperature-controlled environment.</strong> House up to 120 vials in an optional, temperature-controlled environment. Achieve low carryover with active and user programmable needle wash. Variable injection volumes from 0.1 to 100 μL standard, and up to 2 mL optional. Support STAT samples and auto-addition.</li><li><strong>Detection and column options. </strong>Get optional column heating, heating/cooling, or column switching capabilities. Detection capabilities include photodiode array, UV/Vis, fluorescence (FLR), refractive index (RI), evaporative light scattering (ELS), electrochemical, conductivity, and mass detection.</li><li><strong>Intuitive interface and software. </strong>Get instant access to system settings through the large LCD screen with simplified daily set-up with SystemPREP. Supports Empower, MassLynx, and 3<sup>rd</sup> party software packages.</li><li><strong>Compliance-ready. </strong>Supports Waters Empower-based automated system qualification tool (SQT), minimizing the time and cost of annual qualification with standards like GMP, GLP, ISO 17025, and USP &lt;1058&gt;.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_2_47fe8951d2/Waters-Alliance-HPLC-System-2.jpg" alt="Waters Alliance HPLC System: Alliance HPLC System provides powerful functionality for all your application needs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_2_47fe8951d2/thumbnail_Waters-Alliance-HPLC-System-2.jpg 161w," sizes="100vw" width="903" height="874"></p><h3><strong>Specifications</strong></h3><ul><li><strong>Maximum operating pressure</strong>: 5,000 psi</li><li><strong>Front panel display</strong>: Easy to use control panel</li><li><strong>Mobile phases</strong>: Up to 4 with Auto-Blend Technology</li><li><strong>Flow rate range</strong>: Up to 10 mL/min</li><li><strong>Sample carryover</strong>: ≤0.0025% (Caffeine) under specified conditions</li><li><strong>Sample compartment</strong>: 4–40 °C</li><li><strong>Pump options</strong>: Quaternary</li><li><strong>Solvent conditioning</strong>: Vacuum Degas</li><li><strong>Column capacity</strong>: Standard up to 3 columns</li><li><strong>Maximum sample capacity</strong>: 120</li><li><strong>Detector options</strong>:&nbsp;<ul><li>Tunable UV</li><li>Photo Diode Array</li><li>Fluorescence</li><li>Refractive Index</li><li>Evaporative Light Scattering</li><li>Electrochemical</li><li>Conductivity</li><li>Mass Spectrometry</li></ul></li><li><strong>External control</strong>:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627">Empower Software</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/635">MassLynx Software</a></li></ul></li><li><strong>Physical specifications</strong>:<ul><li><strong>Width:</strong> 45.7 cm (18.0 in.) or 58.4 cm (23.0 in.) with column heater</li><li><strong>Height:</strong> 57.1 cm (22.5 in.)</li><li><strong>Depth:</strong> 57.1 cm (22.5 in.) or 64.8 cm (25.5 in.) with sample heater/cooler</li></ul></li></ul></div></div></div></div></div></div></div></div>

Watrex Streamline binary gradient HPLC system

Watrex Streamline™ binary gradient HPLC system consists of two P1 pumps, static mixer, CT1 column thermostat, PDA1 diode-array detector, AS1 auto-sampler, and Clarity PDA datastation.

<p><strong>Streamline™ binary gradient HPLC system consists of two </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=streamline+p1+pump&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>P1 pumps</strong></a><strong>, static mixer, </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/column-thermostats/streamline-ct1-columm-thermostat/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>CT1 column thermostat</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=pda1&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>PDA1 diode-array detector</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/sampling-devices/autosamplers/streamline-as1-autosampler/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>AS1 auto-sampler</strong></a><strong>, and Clarity PDA datastation.</strong></p><h3><strong>FEATURES</strong></h3><ul><li>Optimized pump mechanics with parallel piston design offers <strong>excellent flow rate accuracy</strong> and <strong>gradient composition</strong> at all solvents ratios</li><li><strong>Variable volume injection range 0.1 - 999.9 μl</strong>, high precision and reproducibility, and low carryover AS1 autosapler</li><li>Excellent signal-to-noise ratio of <strong>the PDA2 detector</strong></li><li>Data acquisition, processing and instrument control is by <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><i>Clarity Chromatography Station</i>*</a></li></ul><h3><strong>COMPONENT SPECIFICATION</strong></h3><ul><li>Gradient solvent delivery system <strong>consisting of two P1 HPLC pumps</strong></li></ul><h5><strong>COMPONENT P1 HPLC pumps</strong></h5><ul><li><strong>Flow Rates:</strong> 0,01 ml/min – 9,99 ml/min with step 0.01 ml/min (analytical)</li><li><strong>Flow Rates:</strong> 0,10 ml/min – 40,0 ml/min (preparative)</li><li><strong>Pressure:</strong> 42 MPa/6000 psi (analytical, stainless steel)</li><li><strong>Pressure:</strong> 35 Mpa/5000 psi (analytical, PEEK)</li><li><strong>Pressure:</strong> 10 Mpa/1500 psi (preparative)</li><li><strong>Flow precision:</strong> ± 0,2% RSD @ 1mL/min. and 1,000 psi (analytical)</li><li><strong>Flow accuracy:</strong> ± 1% @ 1mL/min. and 1,000 psi (analytical)</li><li><strong>Pressure accuracy:</strong> ± 2% of full-scale, with 80:20 H2O/IPA @ 1000psi</li><li><strong>Pulsation:</strong> better than ± 0.5% @ 1mL/min. and 1,000 psi (analytical)</li><li><strong>Remote control:</strong> USB</li><li><strong>Remote control:</strong> Start, Stop, Status, Error</li></ul><h5><strong>COMPONENT - PDA1 detector</strong></h5><ul><li><strong>Wetted Materials:</strong> Stainless Steel/ PEEK*, teflon, Glass (*depending on configuration)</li><li><strong>Light Source:</strong> Deuterium Lamp, Tungsten Lamp</li><li><strong>Wavelength Range:</strong> 190 – 720 nm</li><li><strong>Number of Diodes:</strong> 256</li><li><strong>Baseline Noise:</strong> ± 1 x 10-5 AU (@240 nm, 2 sec. risetime)</li><li><strong>Baseline Drift:</strong> less than 3 x 10-4 AU/h</li><li><strong>Wavelength Accuracy:</strong> 0.5 nm</li><li><strong>Mean Pixel Pitch:</strong> 2.2 nm</li><li><strong>Resolution:</strong> 7 nm</li><li><strong>Linearity:</strong> higher than 2.0 AU</li><li><strong>Data Rate:</strong> 1 Hz – 100 Hz</li></ul><h5><strong>COMPONENT - CT1 Column thermostat</strong></h5><ul><li><strong>Temperature range:</strong> 10 °C above ambient temperature to 100 °C</li><li><strong>Temperature accuracy:</strong> max. 0,5 % of actual temperature</li><li><strong>Temperature stability:</strong> RSD &lt; 0,4 %</li><li><strong>Settability:</strong> 0,1 °C</li><li><strong>Internal dimensions:</strong> 320 x 50 x 30 mm (L x W x H)</li><li><strong>Compartment material:</strong> PTFE coated metal</li><li><strong>Compartment connection:</strong> USB</li><li><strong>Control:</strong> build-in color touch panel, datasystem</li></ul><h5><strong>COMPONENT - AS1 autosampler:</strong> X-Y-Z-Operation; motor driven syringe, Capacity: 60 vials, Sample Loop: standard, 20μl, Reproducibility: Fix Volume: &lt; 0.5%, Variable Volume: &lt;1.0%, Carry-over: &lt;0.01% , Programmable wash.</h5><h5><strong>COMPONENT - Clarity™ Chromatography Station:</strong> PDA data acquisition and evaluation, gradient, and autosampler control with pre-set methods for experimental settings. PC Win 10 Professional with LCD monitor .</h5><h3><strong>Ordering information:</strong></h3><h5><strong>PN/Component:</strong></h5><ul><li><strong>SSG101-001</strong> Streamline™ binary HPLC system (Includes P1m+P1s pumps, PDA1 detector, CT1 thermostat, AS1 autosampler, solvent rack, Clarity PC station)</li></ul><h5>Optional configurations:</h5><ul><li><strong>SPDA99-201</strong> Remove PDA1 diode-array detector from system</li><li><strong>ST99-001</strong> Remove CT1 column thermostat from system</li><li><strong>SAS99-001</strong> Remove AS1 autosampler from system</li><li><strong>SUV90-001</strong> Add UV1 Variable UV/Vis detector to system</li><li><strong>SFC90-001</strong> Add FC1 Fraction collector to system</li><li><strong>SCT90-002</strong> Add CT1i column thermostat with manual injector from system</li></ul>

Waters ACQUITY UPC2 System

Waters ACQUITY UPC2 System allows the manipulation of fluids with a wide range of polarities and enables scientists to separate, analyze, and understand compounds that have challenged all other LC and GC technologies.

<div><div><h4><strong>The ease of reversed-phase meets the power of normal-phase</strong></h4></div></div><div><div><p><strong>In reversed-phase chromatography, polar compounds are eluted first, causing routine and complex separation challenges. With convergence chromatography, however, polar compounds are retained and eluted last - providing the separation power of normal-phase liquid chromatography (LC) with the ease-of-use of reversed-phase LC.</strong></p><div><div><div><div><p>Using green and non-toxic <strong>compressed liquid CO₂</strong> as a primary mobile phase, the <strong>ACQUITY UPC2® System</strong> delivers the ability to precisely vary <strong>mobile phase strength, pressure, and temperature</strong>. The ability to <strong>fine-tune the resolving power and selectivity</strong> of the system enables better control over the retention of analytes for <strong>separating, detecting, and quantifying</strong> structural analogs, isomers, and <strong>enantiomeric and diastereomeric mixtures</strong> – all compounds that are often a challenge to separate by any other means.</p><ul><li>The chromatographic principles and selectivity of <strong>normal-phase LC</strong></li><li>The ease of use and method development simplicity of <strong>reversed-phase LC</strong></li><li>The use of <strong>gradients across the widest polarity range</strong></li><li><strong>Chiral and achiral separations</strong> in one system, with unequaled speed and confidence</li></ul></div></div><div><div><h5><strong>Recommended Use</strong></h5><p>To solve routine and complex separations challenges that require<strong> unparalleled selectivity and ease-of-use</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPC_2_System_43f37e1130.jpg" alt="Waters ACQUITY UPC2 System: ACQUITY UPC2 System gives scientists the ability to precisely vary mobile phase strength, pressure, and temperature." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPC_2_System_43f37e1130.jpg 234w," sizes="100vw" width="1024" height="682"></p><div><div><h3><strong>Bringing ease of use to normal-phase LC</strong></h3><p>By providing the ability to precisely vary <strong>mobile phase strength, pressure, and temperature</strong>, the <strong>Waters ACQUITY UPC<sup>2</sup> System</strong> gives scientists the power to better control the retention of analytes for <strong>separating, detecting, and quantifying</strong> structural analogs, isomers, and <strong>enantiomeric and diastereomeric mixtures</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPC_2_System_6_c2a18a19b3.jpg" alt="Waters ACQUITY UPC2 System: Separation of propranolol and hydroxy metabolites. Note that each hydroxy metabolite has an R and S form." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPC_2_System_6_c2a18a19b3.jpg 235w," sizes="100vw" width="1024" height="681"></p></div></div><div><div><h3><strong>The world's first convergence chromatography system</strong></h3><p>Unique in its capabilities, the pioneering <strong>ACQUITY UPC<sup>2</sup> System</strong> delivers what the rest of the industry never could – a system, based on the principles of <strong>normal-phase LC</strong>, with the <strong>ease-of-use of reversed-phase LC</strong>, and purposefully built for analytical performance, placing <strong>convergence chromatography</strong> atop the list of separations tools in the kit of all analytical chemists.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPC_2_System_7_2efdc13cd5.jpg" alt="Waters ACQUITY UPC2 System: UPC2 analysis can be 30x faster and use 75x less solvent per run - all with 100x lower cost per analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPC_2_System_7_2efdc13cd5.jpg 182w," sizes="100vw" width="1024" height="879"></p></div></div><div><div><h3><strong>Unparalleled resolving power and selectivity</strong></h3><p>With its <strong>separation potential for a far broader chemical spectrum</strong> of applications than that of reversed-phase LC-based instrumentation, the <strong>ACQUITY UPC<sup>2</sup> System</strong> leverages <strong>convergence chromatography</strong> to enable superior performance, using <strong>inexpensive, compressed CO₂</strong> as a non-toxic mobile-phase.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPC_2_System_8_655b0ad41e.jpg" alt="Waters ACQUITY UPC2 System: The ACQUITY UPC2-MS FFA analysis provides a simple and fast method with a significant reduction in analysis time compared to alternative techniques such as GC-MS, which requires FAME derivatization." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPC_2_System_8_655b0ad41e.jpg 232w," sizes="100vw" width="1024" height="691"></p></div></div><div><div><h3><strong>Retention mechanisms and orthogonality to RPLC</strong></h3><p>Harnessing what's now known as <strong>convergence chromatography</strong>, an evolution of the traditional <strong>SFC</strong> technique, the <strong>ACQUITY UPC<sup>2</sup> System</strong> has the power to create <strong>selective separations of structurally similar chiral and achiral compounds</strong>.</p><p>The <strong>miscibility of CO₂ with a wide range of polar and non-polar organic solvents</strong> has made the liquid CO₂-based mobile phase versatile enough to separate a much wider range of compounds than reversed-phase LC, especially for mixtures containing polar compounds. Not only can CO₂-based solvents be used with both polar and non-polar stationary phases, but chromatography can be influenced by modulating solvent gradients with a much wider choice of columns using the same <strong>mass spectrometry-compatible co-solvents</strong>.</p><p>Being <strong>orthogonal to reversed-phase LC</strong>, <strong>UP<sup>C2</sup> separations</strong> often show an <strong>inverse order of elution</strong> among groups of analytes. In combination with various detection techniques, this orthogonality is <strong>valuable for confirming analyte identity in complex matrices</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPC_2_System_9_dc03654861.jpg" alt="Waters ACQUITY UPC2 System: With ACQUITY UPC2 System, orthogonality to the most commonly used high/low pH approach in reversed-phase can be clearly seen as a useful addition to the separation challenges faced by synthesis analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPC_2_System_9_dc03654861.jpg 200w," sizes="100vw" width="1024" height="801"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPC_2_System_2_454af0c6e1.jpg" alt="Waters ACQUITY UPC2 System: ACQUITY UPC2 System with ACQUITY QDa Detector." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPC_2_System_2_454af0c6e1.jpg 234w," sizes="100vw" width="1024" height="682"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPC_2_System_3_72c8ad4d63.jpg" alt="Waters ACQUITY UPC2 System: ACQUITY UPC2 System with Xevo TQ-XS." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPC_2_System_3_72c8ad4d63.jpg 234w," sizes="100vw" width="1024" height="682"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPC_2_System_4_e2672f200d.jpg" alt="Waters ACQUITY UPC2 System: ACQUITY UPC2 System with ACQUITY UPLC ELS, ACQUITY UPC2 PDA, and ACQUITY QDa Detectors." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPC_2_System_4_e2672f200d.jpg 234w," sizes="100vw" width="1024" height="682"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPC_2_System_5_18c501a159.jpg" alt="Waters ACQUITY UPC2 System: ACQUITY UPC2 System with Xevo G2-XS QTof." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPC_2_System_5_18c501a159.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div></div></div></div></div></div></div>

Waters Arc Premier LC System

The Arc Premier System features new, innovative MaxPeak High Performance Surfaces (HPS) Technology, which effectively reduces non-specific adsorption due to metal interactions, without complicated mobile phases or laborious methods.

<div><div><h4><strong>See clearly and consistently, with confidence.</strong></h4></div></div><div><div><p><strong>Today’s scientists and lab managers need to confidently produce the most accurate, reliable, and reproducible data. The Arc Premier System produces correct, robust results from the very first injection, reliably and easily.&nbsp;</strong></p><p>The <strong>Arc Premier System</strong> features the new, innovative solution of <strong>MaxPeak High Performance Surfaces (HPS) Technology</strong>, which effectively reduces non-specific adsorption due to metal interaction — without complicated mobile phases or laborious methods. The <strong>Arc Premier System</strong> sharpens chromatographic peaks for <strong>metal-sensitive compounds</strong> by eliminating secondary interactions from metals, resulting in:</p><ul><li>Improved compliance from higher quality data</li><li>Faster, more reproducible, and accurate data integration</li><li>Shorter time from sample to result, without conditioning</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_6d7c95a93a.jpg" alt="Waters Arc Premier LC System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_6d7c95a93a.jpg 245w," sizes="100vw" width="1024" height="611"></p><p>With the Arc Premier System, we continue to deliver on the product quality you expect, combined with recent innovations in <strong>MaxPeak HPS Technolog</strong>y, to always deliver the<strong> best possible results</strong>.&nbsp;</p><p>Whether you are working to ensure the safety of a new and innovative product, maintain the consistency and supply of a current one, or test your food safety and quality, the Arc Premier System gives you<strong> greater data quality, flexibility, reproducibility, and faster data integration </strong>with right first-time results right out of the box, <strong>without the need for complicated mobile phases and laborious methods to prepare your system.</strong></p><div><div><div><div><div><div><ul><li><strong>MaxPeak HPS Technology</strong>, minimizing risk of metal-analyte interactions</li><li>Pressure envelope extends to <strong>9500 psi </strong>through a flow rate range of <strong>1–5 mL/min</strong>, providing an optimized pairing with column dimension and particle size</li><li><strong>Flexible quaternary and binary pumping options</strong></li><li>High performance analytical optical detectors, include photodiode array (PDA), UV/Vis, and fluorescence (FLR)</li><li>Mass detection options with the <strong>ACQUITY™ QDa™ Mass Detector</strong>,<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1543"> <strong>SQD2 Detector</strong></a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/209"><strong>Xevo™ TQ-S micro</strong></a>, and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/212"><strong>Xevo TQ-S cronos</strong></a></li><li>Flexible column manager options available for up to six columns ranging in length from <strong>50 mm to 300 mm</strong>, with a temperature range of <strong>4 °C to 90 °C</strong></li><li><strong>Auto•Blend Plus™ Technology</strong> for mobile phase reproducibility and <strong>Gradient SmartStart</strong> for method transfer</li><li>Wide range of trusted particle technologies in <strong>MaxPeak Premier Columns</strong></li><li>Compliance-ready<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627"><strong>Empower™ Chromatography Data System</strong> </a>(CDS) delivering high quality data integrity</li><li>Automated quantification tools to minimize compliance costs and time</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_2_d1a922ae6d.jpg" alt="Waters Arc Premier LC System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_2_d1a922ae6d.jpg 245w," sizes="100vw" width="1024" height="585"></p></div></div><div><div><h3><strong>Flexible and Robust HPLC Designed for Your Development and Routine Testing Needs</strong></h3><ul><li><strong>Low dispersion HPLC: </strong>High performing HPLC with bandspread of &lt;30 μL for high chromatographic resolution.&nbsp;</li><li><p><strong>Versatile column management: </strong>Support for analytical LC column dimensions up to 300* mm with capacity to automatically select between as many as six analytical columns. Equipped with independently controlled temperature zone, active pre-heating, and<strong> eCord™ Intelligent Chip Technology </strong>for Waters columns.</p><p>* MaxPeak Premier Columns are recommended for use with the Arc Premier System and are currently only available in lengths up to 150 mm.</p></li><li><strong>Flow-through needle Injector: </strong>Volume range of 0.1 to 1000 μL and ultra-low carryover performance compatible with sensitive LC-MS applications.</li><li><strong>High Performance Surfaces Technology: </strong>With MaxPeak HPS Technology, analytes are impeded from Lewis acid-base interactions and adsorptive losses. This technology has proven to be effective in improving the analysis of electron-rich compounds, such as those containing phosphate and carboxylate groups.</li><li><strong>Consumables: </strong>Compatible with MaxPeak Premier Column chemistry to meet the needs of your reversed-phase and HILIC applications. Quality Control (QC) Reference Material benchmarks your Arc Premier System and enables perfect resolution. <strong>Certified clean </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/197"><strong>QuanRecovery™ vials</strong></a> to give full sample integrity.</li><li><strong>High performance analytical detectors : </strong>A wide range of high-performance detection capabilities, optimized with HPS, to support diverse applications. Includes photodiode array, UV/Vis, and fluorescence, with the additional option of single and triple quad mass detection.</li><li><strong>Solvent blending: </strong>Quaternary or binary solvent mixing. Delivers precise and accurate separations at pressures up to 9500 psi. Optional solvent select valve adds access to additional solvents. Automated solvent compressibility, integrated solvent degassing, and programmable seal wash maximize flow accuracy, precision, and reliability.</li><li><strong>Auto•Blend Plus Technology: </strong>Reduce human error and accelerate method robustness testing for chromatographic methods by programming gradients to control mobile phase pH and ionic strength.</li><li><strong>Gradient SmartStart: </strong>Adjust the injection relative to the gradient start to emulate other HPLC systems’ dwell volumes, without the need to alter the gradient table. Successfully transfer most methods in just two injections.</li><li><strong>Laboratory Informatics: </strong>Software control within your existing <strong>Empower </strong>or <strong>MassLynx™ </strong>infrastructure. Empower Method Validation Manager (MVM) allows you to perform chromatographic method validation in one application. Optional S-Matrix Fusion Software automates your method development according to AQbD guidelines.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_3_5db411b90a.jpg" alt="Waters Arc Premier LC System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_3_5db411b90a.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div></div></div><div><div><div><div><div><div><h3><strong>The Universal Platform for Liquid Chromatography</strong></h3><p>Non-specific adsorption of metal-sensitive compounds is an unpredictable challenge, resulting in chromatography with large relative standard deviations (RSDs) and broad, overlapping peaks, which can be challenging to integrate quickly and be accurate consistently.</p><p>Designed with MaxPeak HPS Technology and paired with <strong>MaxPeak Premier 2.5 μm Columns</strong>, the Arc Premier System improves data quality for challenging compounds, including metal-sensitive compounds tested with LC methods – so you can see clearly and consistently, with confidence.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_8_a4038daa52.jpg" alt="Waters Arc Premier LC System: Figure 1. Analysis of adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate. Significant improvements to sensitivity and recovery are observed with the Arc Premier Solution compared to a conventional LC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_8_a4038daa52.jpg 245w," sizes="100vw" width="1123" height="520"></p></div></div></div></div></div></div></div></div><div><div><h3><strong>Clear</strong></h3><p>Future-proof your lab for the next generation of challenging compounds with MaxPeak HPS Technology, which sharpenspeaks for metal-sensitive compounds, resulting in higher data quality and improved sensitivity with chromatographic reproducibility and precision.</p><p>Data quality and performance are <strong>critical </strong>for your lab, but those can’t come at the sacrifice of ruggedness and ease of use.&nbsp;</p><p>With MaxPeak HPS Technology and flexible LC performance, your lab gets the typical benefits of an LC system optimized for <strong>2.x μm column chemistry </strong>with the MaxPeak HPS Technology’s ability to shorten run times and achieve sharp peaks - no lengthy and complex conditioning and conditioning methodologies for metal-sensitive compounds required.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_9_34ec4befec.jpg" alt="Waters Arc Premier LC System: Figure 2. Analysis of adenosine triphosphate. Lower limits of detection and increased calibration curve linearity are demonstrated with the Arc Premier Solution compared to a conventional LC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_9_34ec4befec.jpg 245w," sizes="100vw" width="940" height="523"></p></div></div><div><div><h3><strong>Consistent</strong></h3><p>Seeing your results more clearly than ever is critical, but those results must be consistent and error-free and your methods easily reproducible, so you can spend less time on analysis and ensure compliance.&nbsp;</p><p>By <strong>significantly sharpening peak shapes</strong> for metal-sensitive analytes, scientists report an improvement in %RSD when integrating data from metal-sensitive compounds on the Arc Premier System. Along with the improvement in data consistency users also report a decrease of time needed to integrate peaks by <strong>approximately 30%</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_f80500f8a4.jpg" alt="Waters Arc Premier LC System: Figure 3. Analysis of hydrocortisone phosphate, betamethasone phosphate, dexamethasone, and dexamethasone acetate. Peak heights increased and peak shapes are more symmetrical for metal- sensitive compounds on the Arc Premier Solution compared to a conventional LC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_f80500f8a4.jpg 235w," sizes="100vw" width="1381" height="918"></p><p>The Arc Premier System <strong>eliminates </strong>the need for<strong> variability-prone conditioning and complex mobile phases</strong> – so you can minimize user errors, improve compliance, and reduce expensive audits from red flag-inducing conditioning injections.</p><p>Your organization needs to reliably migrate higher-quality methods between your systems for maximum reproducibility. With MaxPeak HPS Technology and flexible pump configurations, your lab can easily, reliably <strong>migrate methods between MaxPeak HPS-based systems </strong>quickly, saving you time and money from unnecessary rework. You can also<strong> migrate methods from non-MaxPeak HPS-enabled systems </strong>safely, as method performance of non-metal-sensitive compounds have been shown to not be adversely affected.</p><p>Combining higher data quality with ease of use for your analysts and seamless method transfer, enables results that can be generated consistently between runs and with various analysts.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_6_5332aab31f.jpg" alt="Waters Arc Premier LC System: Figure 4. Analysis of hydrocortisone phosphate, betamethasone phosphate, dexamethasone, and dexamethasone acetate. A zoom in on the baseline shows improved peak shapes and resolution for metal-sensitive compounds on the Arc Premier Solution compared to a conventional LC system, improving baseline resolution." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_6_5332aab31f.jpg 229w," sizes="100vw" width="1380" height="942"></p><h3><strong>Confident</strong></h3><p><strong>Reducing analytical uncertainty and achieving peace</strong> of mind are important for your organization. With MaxPeak HPS Technology, metal-sensitive analytes become visible that were previously undetectable. This allows you to quickly and confidently know that you’ve seen everything in your sample, giving you the confidence to stand behind your data-driven decisions.</p><p>The <strong>combination of MaxPeak HPS Technology, flexible pump and column management options, and precise optical and MS detection</strong> give you the method development and verification capabilities to know that no matter the sample - you can deliver a robust method you can stand behind.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_2_85f2f7cc29.jpg" alt="Waters Arc Premier LC System: Figure 5. Analysis of data of hydrocortisone phosphate, betamethasone phosphate, dexamethasone, and dexamethasone acetate from an Arc Premier System and a conventional LC system by six users. Peak area reproducibility improved 9 – 124x when integrating the same data from metal-sensitive compounds across six users." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_2_85f2f7cc29.jpg 239w," sizes="100vw" width="1243" height="810"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_5_514749f32d.jpg" alt="Waters Arc Premier LC System: Figure 6. Analysis of acetaminophen, caffeine, leucine enkephalin, sulfadimethoxine, amitriptyline, reserpine, and terfenadine shows that non-metal-sensitive compounds demonstrate comparable performance between the Arc Premier Solution and conventional LC systems." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_5_514749f32d.jpg 245w," sizes="100vw" width="1242" height="735"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_7_edc8ecaa93.jpg" alt="Waters Arc Premier LC System: Figure 7. Comparison of adenosine triphosphate and adenosine diphosphate peaks using a conventional LC system and the Arc Premier Solution over 120 injections. The peak areas of metal-sensitive compounds remain constant from the first to the 120th injection using the Arc Premier Solution. Peak areas of metal-sensitive compounds on a conventional LC system continuously increase over time and do not stabilize even after 120 injections." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_7_edc8ecaa93.jpg 218w," sizes="100vw" width="1038" height="744"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_3_f1cca337bc.jpg" alt="Waters Arc Premier LC System: Figure 8. Comparison of adenosine triphosphate and adenosine diphosphate peaks using a conventional LC system and the Arc Premier Solution over 120 injections. The peak areas of metal-sensitive compounds remain constant from the first to the 120th injection using the Arc Premier Solution showing less than a 1% change in peak area for all compounds. Peak areas of metal-sensitive compounds on a conventional LC system show an increase of 2–35% when compared to the first injection and show no signs of stabilization." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_3_f1cca337bc.jpg 216w," sizes="100vw" width="1039" height="750"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Arc_Premier_LC_System_4_1dae3909ae.jpg" alt="Waters Arc Premier LC System: Figure 9. Adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, and adenosine stability using the Arc Premier Solution over 1000 injections. The peak areas of metal-sensitive compounds remain consistent over 1000 injection using the Arc Premier Solution demonstrating the robustness of the system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Arc_Premier_LC_System_4_1dae3909ae.jpg 243w," sizes="100vw" width="1040" height="669"></p></div></div></div></div>

Watrex Streamline Isocratic HPLC system

Streamline™ isocratic HPLC system consists of one P1 pump, CT1i column thermostat with manual injector and UV1 UV/Vis detector.

<p><strong>Streamline™ isocratic HPLC system consists of one </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=streamline+p1+pump&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>P1 pump</strong></a><strong>, </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/column-thermostats/streamline-ct1-columm-thermostat/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>CT1 column thermostat </strong></a><strong>with manual injector and </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/?s=uv1+uv&amp;post_type=product&amp;dgwt_wcas=1?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>UV1 UV/Vis detector</strong></a><strong>. Modular concept offers easy and economical upgrades or configuration modifications such as additional detectors, autosampler or fraction collector.</strong></p><h3><strong>FEATURES</strong></h3><ul><li>Optimized pump mechanics with parallel piston design offers excellent flow rate accuracy and low pulsation</li><li>Excellent signal-to-noise ratio of the UV1 detector</li><li>Data acquisition, processing and instrument control is done by Clarity Chromatography Station</li></ul><h3><strong>COMPONENT SPECIFICATION</strong></h3><h5><strong>SPECIFICATIONS - P1 HPLC pump:</strong></h5><ul><li><strong>Flow Rates:</strong> 0.001 to 10.000 mL/min</li><li><strong>Pressure:</strong> 0 to 6,000 psi (42 MPa)</li><li><strong>Flow precision:</strong> &gt; 0.2% RSD</li><li><strong>Flow accuracy:</strong> ± 1 % @ 1mL/min and 7 MPa,</li><li><strong>Pressure accuracy:</strong> ± 2% of full-scale, with 80:20 H2O/IPA @ 7 MPa</li><li><strong>Pulsation:</strong> ± 0.5 % @ 1mL/min and 7 MPa</li><li><strong>Remote inputs:</strong> USB</li></ul><h5><strong>SPECIFICATIONS - UV1 UV detector:</strong></h5><ul><li><strong>Light Source:</strong> Deuterium Lamp, Tungsten Lamp</li><li><strong>Wavelength Range:</strong> 190 – 800 nm</li><li><strong>Baseline Noise:</strong> 1 x 10-5 AU (@240 nm, 2 sec. risetime)</li><li><strong>Baseline Drift:</strong> less than 3 x 10-4 AU/h</li><li><strong>Wavelenghth Reproducibility:</strong> ± 0.1 nm</li><li><strong>Linearity:</strong> better than 2.5 AU</li><li><strong>Analog Output:</strong> 1x 1 V</li></ul><h5><strong>SPECIFICATIONS - CT1 Column thermostat:</strong></h5><ul><li><strong>Temperature range:</strong> 10 °C above ambient temperature to 100 °C</li><li><strong>Temperature accuracy:</strong> max. 0,5 % of actual temperature</li><li><strong>Temperature stability:</strong> RSD &lt; 0,4 %</li><li><strong>Settability:</strong> 0,1 °C</li><li><strong>Internal dimensions:</strong> 320 x 50 x 30 mm (L x W x H)</li><li><strong>Compartment material:</strong> PTFE coated metal</li><li><strong>Compartment connection:</strong> USB</li><li><strong>Control:</strong> build-in color touch panel, datasystem</li></ul><h5><strong>SPECIFICATIONS - </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999"><strong>Clarity™ Chromatography Station</strong></a><strong>:</strong></h5><ul><li>Advanced data acquisition and evaluation, enables routine separations with pre-set methods for experimental settings. PC Win 10 Professional with LCD monitor included</li></ul><h3><strong>Ordering information:</strong></h3><h5><strong>PN/Component:</strong></h5><ul><li><strong>SSI101-001</strong> Streamline™ isocratic system (Includes P1 pump,UV1 detector, CT1i column thermostat with manual injector, solvent rack, Clarity datastation)</li><li><strong>SUV99-001</strong> Remove UV1 detector from system</li><li><strong>ST99-002</strong> Remove CT1i column thermostat with manual injector from system</li><li><strong>SPDA90-001</strong> Add PDA1 265 photodiode detector to system</li><li><strong>SRI90-001</strong> Add RI1 Refractive index detector to system</li><li><strong>SAS90-001</strong> Add AS1 Autosampler to system</li><li><strong>SFC90-001</strong> Add FC1 Fraction collector to system</li></ul>

Thermo Scientific Vanquish Core HPLC System

The Vanquish Core HPLC systems provide exceptional analytical precision, detector sensitivity, and operational simplicity to enable unprecedented reliability and robustness.

Thermo Scientific™ Vanquish™ Neo UHPLC System

Take advantage of outstanding performance and productivity for all LC-MS workflows with nano-, capillary, and microflow using the Thermo Scientific™ Vanquish™ Neo UHPLC system.

Thermo Scientific™ Dionex™ Aquion™ Ion Chromatography (IC) System

Perform basic anion or cation analyses reliably with Dionex™ Aquion™ IC system bundles that include hardware, software, and consumables for easy ordering.

<h4><strong>Reliable, budget-friendly ion chromatography for routine anion and cation analysis</strong></h4><p>Simplify ion analysis in your laboratory with the <strong>Dionex™ Aquion™ IC system</strong>, a compact and easy-to-operate platform designed for routine applications in <strong>environmental, food safety, and academic labs</strong>. Ideal for budget-conscious operations, the Aquion system delivers dependable performance, quick startup, and low operating costs.</p><h3><strong>Key system features</strong></h3><ul><li><strong>Chemically inert PEEK flow path</strong></li><li><strong>Serial dual-piston pump</strong><ul><li>Flow rate: 0.01–5.0 mL/min</li><li>Pressure: up to 5000 psi</li></ul></li><li><strong>High-pressure pulse dampener</strong></li><li><strong>Electrically actuated, PEEK, 6-port Rheodyne injection valve</strong></li><li><strong>Conductivity detection with digital signal processing</strong><ul><li>Range: 0–15,000 μS</li><li>Temperature-controlled conductivity cell for signal stability</li></ul></li><li><strong>Support for electrolytic or chemical suppressors</strong></li><li><strong>Optional column heater</strong></li><li><strong>Built-in inline vacuum degasser</strong></li></ul><h3><strong>Benefits of electrolytic suppression</strong></h3><ul><li>Lower background conductivity and reduced detection limits</li><li>Faster system startup</li><li>Superior sensitivity for both anion and cation analysis</li><li>No need for manual regenerant preparation</li></ul><h3><strong>Optimized detection with conductivity</strong></h3><p>The Aquion system is optimized for conductivity detection using a <strong>thermostated high-performance cell</strong> that ensures stability against ambient temperature fluctuations. Digital signal control offers a wide dynamic range for simultaneous detection of analytes with varying concentrations.</p><h3><strong>Seamless control with Chromeleon™ CDS software</strong></h3><p>Included in every system bundle, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)</strong></a> software provides intuitive instrument control, automated workflows, and streamlined data handling:</p><ul><li><strong>eWorkflows</strong>: auto-load instrument conditions and processing templates</li><li><strong>Calibration Wizard</strong>: step-by-step guidance for method setup and quantification</li><li><strong>Wellness Trending</strong>: monitor system performance over time for predictive maintenance</li></ul><h3><strong>Anion and Cation System Bundles</strong></h3><p>Each bundle includes everything needed to get started:</p><h4><strong>Included in all system bundles:</strong></h4><ul><li>Dionex Aquion IC System with power cable</li><li>Dionex Aquion Analysis Kit (anion or cation specific)</li><li>Chromeleon SE software and license</li><li>3-year warranty (parts-only coverage in years 2 and 3)<br>→ Optional extended warranty available including labor and travel in years 2 and 3</li></ul><h4><strong>Anion Analysis Kit includes:</strong></h4><ul><li><strong>Dionex IonPac AG22 (4 × 50 mm) guard column</strong></li><li><strong>Dionex IonPac AS22 (4 × 250 mm) analytical column</strong></li><li><strong>Dionex AERS 500 Carbonate (4 mm) suppressor</strong></li><li><strong>Combined Seven Anion Standard II</strong><br><i>Note: Eluent concentrate must be ordered separately.</i></li></ul><h4><strong>Cation Analysis Kit includes:</strong></h4><ul><li><strong>Dionex IonPac CG12A (4 × 50 mm) guard column</strong></li><li><strong>Dionex IonPac CS12A (4 × 250 mm) analytical column</strong></li><li><strong>Dionex CDRS 600 (4 mm) suppressor</strong><br><i>Note: Eluent concentrate and standards must be ordered separately.</i></li></ul><h3><strong>Why choose Dionex Aquion?</strong></h3><ul><li>Proven IC platform with straightforward operation</li><li>Reliable results and stable performance</li><li>Full compatibility with standard and microbore column formats (2–5 mm)</li><li>Low cost of ownership with high-quality support and service</li><li>Ideal entry point for labs new to ion chromatography</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Dionex_Aquion_Iontovy_Chromatograf_2_8c661a76f1/Thermo-Dionex-Aquion-Iontovy-Chromatograf-2.jpg" alt="Thermo Scientific™ Dionex™ Aquion™ Ion Chromatography (IC) System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Dionex_Aquion_Iontovy_Chromatograf_2_8c661a76f1/thumbnail_Thermo-Dionex-Aquion-Iontovy-Chromatograf-2.jpg 156w," sizes="100vw" width="650" height="650"></p>

Thermo Scientific Dionex Integrion HPIC System 

Achieve versatile and efficient separations that meet your routine ion analysis demands with the reliable Thermo Scientific Dionex Integrion HPIC system. 

<div><div id="container-9240b945f2"><div><div id="title-b4d119a2b1"><h3 data-uw-rm-heading="level" role="heading" aria-level="2"><strong>Dionex Integrion HPIC System: An easy solution for routine analysis</strong></h3></div></div><div><div id="container-2c24fcced5"><div><div id="text-1819779492"><p><strong>Achieve versatile and efficient separations that meet your routine ion analysis demands with the reliable Thermo Scientific Dionex Integrion HPIC system. Its high-pressure ion chromatography (HPIC) capabilities enable faster analysis without compromising data quality. The Dionex Integrion&nbsp;HPIC system is designed for routine&nbsp;ion chromatography (IC) applications for water, food and beverage, biofuels and pharmaceutical analysis.</strong></p><figure class="image"><img style="aspect-ratio:650/650;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Dionex_Integrion_Iontovy_Chromatograf_2_7a7f2ce86a/Thermo-Dionex-Integrion-Iontovy-Chromatograf-2.jpg" alt="Thermo Scientific Dionex Integrion HPIC System " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Dionex_Integrion_Iontovy_Chromatograf_2_7a7f2ce86a/thumbnail_Thermo-Dionex-Integrion-Iontovy-Chromatograf-2.jpg 156w," sizes="100vw" width="650" height="650"></figure></div></div></div></div></div></div><div><div id="container-ee4e409cf5"><div><div id="sectiontitle-d7eca6bc0f"><h3><strong>Highlights</strong></h3></div></div><div><div id="container-94710c6880"><div><div><div id="container-006293a352"><div><div><div id="text-328176320"><h4><strong>Simple plumbing layout</strong></h4><p><strong>The plumbing layout </strong>of this high-pressure ion chromatography system is based on a logical flow path for <strong>easy component access</strong> in a compact platform with a small physical footprint.</p></div></div></div></div></div><div><div id="container-9133f3a75a"><div><div><div id="text-1491634964"><h4><strong>Inert, reliable components</strong></h4><p><strong>Inert, nonmetallic PEEK components</strong> throughout the <strong>Dionex Integrion HPIC system </strong>ensure <strong>compatibility with corrosive eluents and analyses free from metal contamination.</strong> Easy-to-install<strong> IC PEEK Viper fittings</strong> have <strong>low dead volume</strong> and require no special training or tools.</p></div></div></div></div></div><div><div id="container-1d936b69ba"><div><div><div id="text-644579526"><h4><strong>Wide range of IC columns</strong></h4><p>The Dionex Integrion HPIC system is<strong> compatible with a wide variety of column sizes,</strong> including<strong> smaller-particle-size 4 µm columns for HPIC capabilities, </strong>which provide faster run times and better resolution, to meet the requirements of your applications. &nbsp;</p><figure class="image"><img style="aspect-ratio:650/650;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Dionex_Integrion_Iontovy_Chromatograf_3_bf2f665361/Thermo-Dionex-Integrion-Iontovy-Chromatograf-3.jpg" alt="Thermo Scientific Dionex Integrion HPIC System " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Dionex_Integrion_Iontovy_Chromatograf_3_bf2f665361/thumbnail_Thermo-Dionex-Integrion-Iontovy-Chromatograf-3.jpg 156w," sizes="100vw" width="650" height="650"></figure></div></div></div></div></div><div><div id="container-f643098693"><div><div><div id="text-1278873072"><h4><strong>Automated Eluent Generation (EG)</strong></h4><p>Optional <strong>Reagent-Free Ion Chromatography</strong> with Eluent Generation (RFIC-EG) combines<strong> automated eluent generators and electrolytically regenerated suppressors</strong> to create eluents and regenerants.</p></div></div></div></div></div></div></div></div><div><div id="container-1021b10e17"><div><div><div id="container-a6d8f5fdb8"><div><div><div id="text-1022646706"><h4><strong>Flexible detector options</strong></h4><p>The Dionex Integrion HPIC system allows for easy, swappable integration with either a <strong>conductivity detector</strong> <strong>(CD)</strong> or an <strong>electrochemical detector (ED)</strong>. The system is also compatible with<strong> various external detectors</strong>, including a <strong>mass spectrometer (MS) </strong>or a<strong> variable wavelength detector (VWD).</strong></p></div></div></div></div></div></div></div></div><div><div id="container-cd507ea43e"><div><div><div id="container-3bdbc48a4f"><div><div><div id="text-1802479128"><h4><strong>CDS Software</strong></h4><p>The Dionex Integrion HPIC system comes with the easy-to-use <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>Thermo Scientific Chromeleon Chromatography Data System (CDS)</strong></a><strong>.</strong> This software platform provides powerful control and data processing for IC and offers eWorkflows that make all our essential applications easily accessible.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Dionex_Integrion_Iontovy_Chromatograf_a_ICS_4000_c7b3adbeea/Pragolab-Thermo-Dionex-Integrion-Iontovy-Chromatograf-a-ICS-4000.jpg" alt="Thermo Scientific Dionex Integrion HPIC System " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Dionex_Integrion_Iontovy_Chromatograf_a_ICS_4000_c7b3adbeea/thumbnail_Pragolab-Thermo-Dionex-Integrion-Iontovy-Chromatograf-a-ICS-4000.jpg 236w," sizes="100vw" width="965" height="638"></p></div></div></div></div></div></div></div></div></div></div>

Thermo Scientific Dionex ICS-6000 HPIC System

The modular Thermo Scientific Dionex ICS-6000 HPIC system is a highly configurable ion chromatography (IC) system with high pressure capabilities.

<h3><strong>Dionex ICS-6000 HPIC System: Run different methods simultaneously</strong></h3><p><strong>The modular Thermo Scientific Dionex ICS-6000 HPIC system is a highly configurable ion chromatography (IC) system with high pressure capabilities. It enables you to develop and run different methods for a single sample or for different samples simultaneously. The robust design of this system enables operation at up to 5000 psi and produces consistent, reliable results. As a top-of-the-line ion chromatography system, the Dionex ICS-6000 HPIC system is designed to adapt to the needs of users and push the boundaries of what is possible in ion analysis.</strong></p><figure class="image"><img style="aspect-ratio:650/650;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Dionex_ICS_6000_Iontovy_Chromatograf_3_cd832a923f/Thermo-Dionex-ICS-6000-Iontovy-Chromatograf-3.jpg" alt="Thermo Scientific Dionex ICS-6000 HPIC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Dionex_ICS_6000_Iontovy_Chromatograf_3_cd832a923f/thumbnail_Thermo-Dionex-ICS-6000-Iontovy-Chromatograf-3.jpg 156w," sizes="100vw" width="650" height="650"></figure><h3><strong>Highlights</strong></h3><h4><strong>Flexible configurations</strong></h4><p>Dionex ICS-6000 HPIC systems<strong> come in a variety of configurations</strong>, with the ability operate <strong>as a single- or dual-channel system</strong> to run samples concurrently. This high-pressure IC system can address a full range of IC applications, from <strong>2-D IC</strong> for trace-level analysis to<strong> high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) </strong>for complex carbohydrate analysis.</p><h4><strong>Wide range of IC columns</strong></h4><p>The Dionex ICS-6000 HPIC system is compatible with<strong> 4 μm-particle-size IC columns,</strong> which are available in <strong>150 or 250 mm lengths.</strong> The 150 mm-length columns decrease run times while maintaining chromatographic resolution. The 250 mm-length columns offer improved peak resolution.</p><h4><strong>Automated Eluent Generation (EG)</strong></h4><p>For unmatched method reproducibility and accuracy, the system can electrolytically generate <strong>high-purity hydroxide, carbonate, bicarbonate, or methanesulfonic acid (MSA) eluents </strong>when provided with a source of high-purity deionized water. Using the <strong>Dual EGC mode</strong>, the Dionex ICS-6000 HPIC system can <strong>also generate potassium hydroxide/potassium methanesulfonate (KOH/KMSA) eluents </strong>for complex carbohydrate analysis.</p><figure class="image"><img style="aspect-ratio:1246/940;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_ICS_6000_HPIC_System_66f622c7f9.jpg" alt="Thermo Scientific Dionex ICS-6000 HPIC System: Feutin alditol oligosaccharide analysis using (a) manually prepared eluents (NaOH/NaOAc) (b) Dual EGC mode (KOH/KMSA) with 1 mm column format (c) Dual EGC mode (KOH/KMSA) with 0.4 mm column format." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_ICS_6000_HPIC_System_66f622c7f9.jpg 206w," sizes="100vw" width="1246" height="940"></figure><h4><strong>Versatile detection capabilities</strong></h4><p>The system is<strong> </strong>compatible with a wide variety of detectors, including <strong>suppressed conductivity, electrochemical, UV-Vis absorbance, inductively coupled plasma mass spectrometry (ICP-MS), and mass spectrometry (MS).</strong></p><h4><strong>Mass spectrometry compatible</strong></h4><p>The Dionex ICS-6000 HPIC system is the<strong> ideal IC system to work in tandem with mass spectrometry, </strong>as it can used for a wide variety of sample analyses. From single quadrupole to high resolution accurate mass (HRAM) <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=5&amp;manufacturerItems=3&amp;search=Orbitrap"><strong>Thermo Scientific Orbitrap mass spectrometers</strong></a>, IC-MS analysis provides improved selectivity and higher sensitivity.</p><figure class="image"><img style="aspect-ratio:1245/682;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_ICS_6000_HPIC_System_3_ecfef0173d.jpg" alt="Thermo Scientific Dionex ICS-6000 HPIC System: HRAM MS/MS fragments facilitate identification of unknown compounds. IC, combined with HRAM mass spectrometry, is an ideal solution for component identification in non-targeted and unknown workflows. In this figure, identification of lithium-ion battery failure analysis products using HRAM MS/MS offers four-decimal-point accuracy to distinguish between species. The unique fragmentation signature enhances that confidence." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_ICS_6000_HPIC_System_3_ecfef0173d.jpg 245w," sizes="100vw" width="1245" height="682"></figure><figure class="image"><img style="aspect-ratio:1362/727;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_ICS_6000_HPIC_System_2_64749e2b9d.jpg" alt="Thermo Scientific Dionex ICS-6000 HPIC System: Targeted MS/MS analysis enables quantitation of molecules in a wide variety of matrices. IC, combined with triple quadrupole mass spectrometry, is an ideal solution for resolving molecules that are often difficult to separate from a matrix using conventional liquid chromatography techniques. In this chromatogram, glyphosate in drinking water is detected at a level of 5 ng/L. The linearity of the assay allows quantitation over 3 orders of magnitude, from 5 ng/L to 1000 ng/L, enabling a wide detection range without having to dilute samples." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_ICS_6000_HPIC_System_2_64749e2b9d.jpg 245w," sizes="100vw" width="1362" height="727"></figure><h4><strong>CDS Software</strong></h4><p>The Dionex ICS-6000 HPIC system comes with the<strong> easy-to-use Thermo Scientific Chromeleon Chromatography Data System (CDS)</strong>. This software provides powerful control and data processing for IC and offers eWorkflows that make all our essential applications easily accessible. Additionally,<strong> the Chromeleon CDS can control mass spectrometry systems, enabling seamless IC-MS analyses.</strong></p>

Products from category Liquid chromatographs - page 5

Related content

Science on the Front Lines: Cleanup of a Benzene Spill

The Future of Ion Chromatography | PFAS Testing, EPA 1621 & AOF Explained

Vobořil: There is still much to learn about the immune system