In the week from October 26, 2020, the following webinars await you in the field of LC and LCMS. More <a href="https://lcms.labrulez.com/webinars">details and registration can be found in the WEBINARS section.</a>:
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1. LCGC/Agilent Technologies: Characterization of Viral Vector Particles with LC–MS
2. Separation Science/Waters Corporation: SEE What You&#39;ve Been Missing with ACQUITY PREMIER Columns: Precision Chemistries for Particles and Surfaces
3. Waters Corporation: Optimising Sample Preparation
4. Thermo Fisher Scientific: Global IC Virtual Symposium - Day 3
5. Phenomenex: Sample Preparation Options for Biological Samples
6. Agilent Technologies: OpenLab CDS-The future of Agilent Chromatography Data System for HPLC, GC, GCMS and LCMS 
7. Phenomenex: Halloween Webinar Series: Ghost Peaks &amp; Friends (Peak Issues)
8. SelectScience/Agilent Technologies: Pesticide residue analysis in cannabis: Method fundamentals and confirmation strategies
9. LCGC/Tosoh Bioscience: Simple and Fast: Novel Fc-Receptor Affinity Chromatography for Antibody Characterization
10. Agilent Technologies: The New RapidFire 400: A Complete Approach to High-Throughput Mass Spec for Drug Discovery to Synthetic Biology
11. Technology Networks/SCIEX: Ensuring Safety and Efficacy of Biotherapeutics Through In-Depth LC-MS Sequence Variant Analysis
12. Phenomenex: Halloween Webinar Series: Trick or Treat! Sugar Analysis
13. Separation Science/Tosoh Bioscience: Advanced Technologies Combined for Enhanced GPC/SEC Analysis of Polymers
14. Agilent Technologies: The Weapons of Analytical Chemistry deployed in the War against Food Fraud
15. LCGC/Phenomenex: Get Low: Achieve Less Background Interference and Lower Limits of Detection Using Sample Preparation
16. Technology Networks/Thermo Fisher Scientific: Targeted Proteomics Quantitative Workflows: Considerations for Success
17. SelectScience/Phenomenex: HPLC and UHPLC reversed-phase method development - Selecting a reproducible starting point
In the week from November 2, 2020, another 9 webinars are waiting for you in the field of LC and LCMS.

LC and LCMS webinars - Week 44

The compounds with the same electrochemical manners as epicatechin and catechin and oligomeric proanthocyanogens (OPCs) related to catechin were distinguished from the other compounds according to their electrochemical responses on lower potential (500 mV). The total content of these OPCs including catechin and epicatechin was determined as an equivalent of concentration of the catechin used as an external standard. The results were correlated partly with the results obtained by using of the group analysis for determination of the total polyphenol and for the determination of the anthocyanogens, partly with results obtained by using of the modern method electron spin resonance (ESR) for determination of the antioxidant activity.
<h3>1 INTRODUCTION</h3>
The hop plant (Humulus lupulus L.) from Cannabinaceae family contains beside primary compounds responsible for basic sensory features (bitterness, fragrance and fullness) also many compounds of health importance. There are especially a group of dimers and trimers, antioxidants (1) derived from catechin and epicatechin, socalled oligomeric proanthocyanogens (OPCs) and a group of prenylated flavonoids in hops (2.3), of which xanthohumol is the most abundant 0.5–1.1 %).
The both groups of these compounds have healing ability and protect human organism against development of many civilization diseases. The group of antioxidants OPCs with lower potentials to their oxidation shields the organism against oxidation of DNA and so protect human organism against tumor diseases (4). Flavonoids are perceived by human receptors like hormon oestrogen, thus these compounds show the oestrogenic effect (5,6). The oestrogenic activity of these compounds is using to the menopause anesis and thus return the organism to normal state. These compounds are also very effective for osteoporosis therapy. Prenylflavonoids are oxidized by higher potential (daidzein, genistein) so their contribution to antioxidant processes is lower and their main activities are interactions with tissue receptors influencing the oestrogenic activities and regulation of cell division (7).
The electrochemical detection of CoulArray detector enables a resolution of the electrochemically different groups of compounds based on their voltametric behavior.
The selectivity of this detection enables to analyze the target group of compounds (8) and we can distinguish analytes with lower oxidative potential 400–500 mV ( catechin and from it derived OPCs) from analytes with higher oxidative potential, e.g. ferulic acid (700 mV) or 4-hydroxybenzoic acid or 4-hydroxyphenylacetic acid (900 mV) (9).
The aim of this study was the determination of antioxidant activity of hop oligomeric proanthocyanogens by the method of liquid chromatography with CoulArray detection and comparison with results obtained partly by modern method electron spin reverberation (ERS), partly by classical methods for determination of total polyphenols and determination of anthocyanogens.
<h3>2 MATERIAL AND METHODS</h3>
The objects of this study were hop varieties Harmonie, Rubín and new varieties code-named 4816, 4849 and 5008. 
With regard to sufficient polarity of oligomeric proanthocyanogens and good water solubility, we used the cold hop leaches in this study.
Due to complexity and unknown chemical structures of particular components of hop oligomeric proanthocyanogens so far, the fingerprint technique was chosen for evaluation. All OPCs components eluated in range from 20 to 40 min. were evaluated on potential 500 mV (Fig. 1). The sum of concentrations of all OPCs was expressed using of the concentration of the catechin used for the calibration. The values obtained in this way were correlated with values obtained by other methods: modern ESR-DPPH and classical methods for determination of total polyphenols and determination of anthocyanogens. 
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Obr_1_Chromatogram_merene_frakce_OPC_9427a14b7e/Obr.-1-Chromatogram-merene-frakce-OPC.jpg" alt="Fig. 1 Chromatogram of measured fraction of OPCs"/>
The samples for all analytical methods were prepared by cold leaching: 5 g of milled hops were agitated in 250 ml of distilled water at laboratory temperature for 30 minutes. The leach was centrifuged (15 minutes, 10 000 rpm) and stored by freezing before analysis.
<h3>HPLC</h3>
<h5>Sample preparation</h5>
Hop leach was diluted in ratio of 1:1 with mobile phase A used for chromatography separation and filtrated through cellulose syringe filter 0.20 μm (Chromafil RC-20/25, Macherey Nagel) into chromatographic vials.
<h5>Chromatographic conditions</h5>
The buffer 0.005 M ammonium acetate in ultraclean water, TOC &lt; 5ppb was used for preparation of both phases A and B. The phase A contained 5 % acetonitrile (gradient grade, Sigma Aldrich), the phase B contained 50 % acetonitrile of the same quality. Both phases were filtered through membrane filter Nylon 66, 0,2 μm (Supelco) and pH was adjusted to 3.00 by formic acid (Fluka). All chemicals were for MS application.
<h5>Column</h5>
Purospher STAR RP-18e (5 μm) 250 x 4.6 mm (Merck) Gradient: 0–10 min. 0% B, 10–18 min. 0–8 % B, 18–40 min. 8–10 % B, 40–77 min. 10–21 % B, 77–120 min 21–85 % B. The content of mobile phase B was increased to 100 %. The measuring cells were cleaned and the 15 min equlibration by phase A was applied afterwards. 
<ul>
<li>Temperature of column: 35 °C.</li>
<li>Flow: 0.8 ml/min.</li>
<li>Injection of the sample: 10 μl.</li>
<li>The measuring potentials setting in turn 250, 300, 400, 500, 600, 700, 800 and 900 mV.</li>
</ul>
<h5>Calibration</h5>
The catechin was chosen as the equivalent to express the concentration of all compounds with the same antioxidant activity on reaction potential 500 mV The standard solutions of catechin 0.01; 0.1; 0.5; 1.0 and 5.0 mg/l were used for the external calibration. The determination of all OPCs was realized by the measuring and checking-out of heights of all peaks with the same electrochemical features like catechin. The concentration of OPCs was expressed as catechin (mg/l) according to external calibration. 
<h5>Determination of the antioxidant activity DPPH</h5>
The antioxidant activity of individual hops was determined using the technique of electron spin resonance (ESR). The method is based on the reaction of the stable radical of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) with antioxidants present in the sample.
The antioxidant activity of samples (ARA 2) is expressed as the decline of the concentration of DPPH after 10 minutes of the reaction.
All the measurements of the free radicals were performed on the Spectrometer MiniScope MS 200, Magnettech GmbH, Germany.
The assay: the aliquot of 14 ml of the DPPH stock solution is mixed with 1 ml of the sample in the test tube without delay. The tube is then inserted into the autosampler which is tempered to 30 °C. The measuring program controlling the transport of the reaction mixture in the interval of 1 minute is then immediately triggered. The value of the free radical DPPH signal is then monitored during the following 10 minute interval. After completion of the analysis the measured values are processed by the particular analytical software of the spectrometer. The result of these calculations is a dependence of the value of the DPPH signal of the sample on time. The antioxidant activity ARA2 is expressed in the percent proportion of the DPPH value decline after the 10 minute reaction.
<h5>Determination of the total polyphenol</h5>
Total polyphenols were determined according to EBCAnalytica (10).
<h5>Determination of the anthocyanogens</h5>
Anthocyanogens were determined according to „Pivovarskosladařská analytika“ (11]).
<h3>3 RESULTS AND DISCUSSION</h3>
The correlations of results obtained by described electrochemical method with CoulArray detection and results obtained using methods: ESR – DPPH (R 2 = 0,9412), determination of total polyphenols (R2 = 0,9867) and determination of anthocyanogens (R2 = 0,991) were found. These correlations are shown in Fig. 2, 3, 4. Coefficients of determination were calculated using of statistical programme Microsoft Office Excel 2003.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Obr_2_Elektrochemicka_metoda_s_Coul_Array_detekci_v_porovnani_s_metodou_ESR_DPPH_pro_kvantitativni_vyjadreni_antioxidacni_aktivity_matrice_e7cf9ce546/Obr.-2-Elektrochemicka-metoda-s-CoulArray-detekci-v-porovnani-s-metodou-ESR-DPPH-pro-kvantitativni-vyjadreni-antioxidacni-aktivity-matrice.jpg" alt="Fig. 2 Comparison of electrochemical method with CoulArray detection and ESR-DPPH method for quantification of antioxidative
activity of hop matrix"/>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Obr_3_Elektrochemicka_metoda_metoda_s_Coul_Array_detekci_v_porovnani_s_metodou_pro_stanoveni_celkovych_polyfenolu_jako_mira_antioxidacniho_1b3ffab13c/Obr.-3-Elektrochemicka-metoda-metoda-sCoulArray-detekci-v-porovnani-s-metodou-pro-stanoveni-celkovych-polyfenolu-jako-mira-antioxidacniho.jpg" alt="Fig. 3 Comparison of electrochemical method with CoulArray detection and method for determination of total
polyphenols as a rate of antioxidative potential hop matrix"/>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Obr_4_Elektrochemicka_metoda_metoda_s_Coul_Array_detekci_v_porovnani_s_metodou_pro_stanoveni_anthokyanogenu_jako_mira_antioxidacniho_bc81bd706a/Obr.-4-Elektrochemicka-metoda-metoda-s-CoulArray-detekci-v-porovnani-s-metodou-pro-stanoveni-anthokyanogenu-jako-mira-antioxidacniho.jpg" alt="Fig. 4 Comparison of electrochemical method with CoulArray detection and method for determination anthocyanogens
as a rate of antioxidative potential hop matrix"/>
The newly developed chromatographic method with electrochemical detection can be used to comparison of hops and similar materials with antioxidant activity. This method can be used for evaluation of antioxidant activity like the modern ESR-DPPH method and the classical methods for determination of total polyphenols and for determination of anthocyanogens.
But the best match (R2 = 0,991) was achieved with the method for determination of anthocyanogens, this group of compounds is similar to catechins and their oligomers due to structures and substituents on benzene ring. The correlative coefficients with other methods reflect the enforcement other compounds with antioxidant activity, nevertheless their values 0.9867 and 0.9412 confirm a good agreement. It can be concluded that the main groups responsible for the hop antioxidant features are catechins, their oligomers and anthocyanogens. Other structures like ferulic acid, p-hydroxybenzoic and 4-hydroxy - phenylacetic acids have the lower contribution to the antioxidant activity due to their higher oxidative potentials.

Hops - an abundant source of antioxidants. Methods to assessment of antioxidant activity of hop matrix

In the week from November 2, 2020, the following webinars await you in the field of LC and LCMS. More <a href="https://lcms.labrulez.com/webinars">details and registration can be found in the WEBINARS section.</a>:
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2. LCGC/Sciex/BioPharm: Intact and Subunit MAM Assays Accelerate the Development of Antibody-Drug Conjugates
3. Agilent Technologies: Solving Long Term Data Storage: Tools to Help Labs Migrate to Digital Workflows
4. SelectScience/Agilent Technologies: Expand your laboratory capabilities with the Agilent Stream Select LC/MS system
5. Phenomenex: Overview of Sample Preparation Options for the Pharmaceutical Industry
6. Technology Networks/Bruker: Measuring Stromal Heterogeneity in Cancer Tissues by Tissue Imaging Proteomics
7. Phenomenex: Your Favorite C18 Is Not a Method Development Toolkit
8. Agilent Technologies: From A to K: How to Perform Successful Vitamins Analysis of Health Supplements, Infant Formulas, Foods and Feeds
9. Separation Science/Agilent Technologies: Getting your LC/GC Lab Up and Running Again
10. Agilent Technologies: “A” is for Analytical to Preparative Chromatography
In the week from November 9, 2020, another 11 webinars are waiting for you in the field of LC and LCMS.

LC and LCMS webinars - Week 45

In the week from November 9, 2020, the following webinars await you in the field of LC and LCMS. More <a href="https://lcms.labrulez.com/webinars">details and registration can be found in the WEBINARS section.</a>:
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3. Agilent Technologies: OpenLab CDS Introductory Demonstration
4. LCGC/PerkinElmer: Maximizing Your HPLC Method Development
5. SelectScience/Waters Corporation: Overcoming key challenges in LC-MS quantitation to improve workflow efficiency
6. Agilent Technologies: Benefits of Virtualization and Cloud Environments 
7. SelectScience/SCIEX: Complete solutions for nitrosamine analysis in different sartans and metformin drug formulations by LC-MS/MS
8. SCIEX: Characterization of liposomes as a drug delivery vehicle
9. Separation Science/Merck: Advances in Liquid Chromatography Separations Employing Carbon Particles
10. Separation Science/Biotage: Extraction of New and Emerging Drugs of Abuse Prior to LC-MS or GC-MS Analysis
11. The Analytical Scientist/Waters Corporation: Ensuring data quality and addressing chromatographic challenges in routine pharmaceutical analysis: Tools for Success
12. Technology Networks//SCIEX: Development of a Sensitive SWATH™ MS Workflow To Advance the Characterization of CAR T-Cell Therapies
13. LCGC/Phenomenex: Miniaturized Separations and Increased Sensitivity—What to Expect When Moving to Micro- and Nano-Scale HPLC
14. Agilent Technologies: “F” is for Fractionation: Advanced Topics in Fraction Collection using Agilent’s InfinityLab Purifcation Solutions
15. LCGC/PerkinElmer: What’s in your Water? Monitoring of Per- and Polyfluoroalkyl Substances (PFAS) in Drinking Water According to EPA Method 537.1 by LC-MS/MS
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LC and LCMS webinars - Week 46

In the week from November 16, 2020, the following webinars await you in the field of LC and LCMS. More <a href="https://lcms.labrulez.com/webinars">details and registration can be found in the WEBINARS section.</a>:
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2. Thermo Fisher Scientific: Proteomics session 4: Targeted proteomics
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4. Phenomenex: HILIC - A Systematic Approach
5. Thermo Fisher Scientific: Metabolomics session 5: Compound Discoverer
6. Thermo Fisher Scientific: Proteomics session 5: Proteome Discoverer
7. LCGC/Agilent Technologies/Avantor/Merck group/Tosoh Bioscience: Biopharmaceutical Analysis Focus 2020: A Virtual Symposium - DAY 1
8. Technology Networks/Bruker: MALDI-MS-based assays for cellular drug action and transport with rapifleX MPP
9. SelectScience/Waters Corporation: Improved LC-MS analysis of oligonucleotides with next-generation chromatography
10. Agilent Technologies: Advanced Agilent QTOF Acquisition Utilities
11. SelectScience/Thermo Fisher Scientific: European LC-MS/MS Clinical Laboratory Virtual Day
12. Shimadzu Scientific Instruments: Shimadzu&#39;s LC Method Development (RP) Series - Session 1: Method Screening
13. LCGC/Agilent Technologies/Avantor/Merck group/Tosoh Bioscience: Biopharmaceutical Analysis Focus 2020: A Virtual Symposium - DAY 2
14. Separation Science/SCIEX: Extractables and Leachables in Food Contact Material
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16. LCGC/Thermo Fisher Scientific: Using Combustion Ion Chromatography to Screen for Extractable Organic Fluurine in PFAS, PFAS, FTOH, and FOSA in Water Monitoring
17. Agilent Technologies: WHEN Should I Change That: Ensuring Proper Instrument Performance
18. Agilent Technologies: Practical Approaches to Preparative Chromatography
19. LCGC/Waters Corporation: Applying ISO 10993-18 on Extractables &amp; Leachables Characterization - Supporting the Medical Device Regulatory Pathway

LC and LCMS webinars - Week 47

In the week from November 23, 2020, the following webinars await you in the field of LC and LCMS. More <a href="https://lcms.labrulez.com/webinars">details and registration can be found in the WEBINARS section.</a>:
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4. Phenomenex: Vitamins Analysis
5. Technology Networks/Bruker: 4D-Lipidomics: Searching for the Fountain of Youth
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LC and LCMS webinars - Week 48

In the week from November 30, 2020, the following webinars await you in the field of LC and LCMS. More <a href="https://lcms.labrulez.com/webinars">details and registration can be found in the WEBINARS section.</a>:
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2. PerkinElmer: Advanced Quality Assured Strategies for Multi Mycotoxin Analysis in Food
3. Wiley/MERCK: Mass Spectrometric Analysis of Vitamin D Metabolites
4. Wiley/MERCK: Reshaping the Lipid Science Landscape
5. VWR: Back To Basics: Fundamental Concepts of HPLC &amp; UHPLC
6. Separation Science/Agilent Technologies: After the Shut Down: Back to Business as Unusual
7. Phenomenex: How to Simplify and Optimise Your Traditional Reversed-Phase SPE Process
8. SelectScience/Waters Corporation: Compliance-ready intact mass analysis of modified oligonucleotides and impurities using the BioAccord LC-MS System
9. Thermo Fisher Scientific: Tips and tricks to select HPLC columns
10. PerkinElmer: PerkinElmer Medical LC-MS/MS Seminar at UToledo
11. Thermo Fisher Scientific: Expanding the biomolecular structural analysis capabilities in XlinkX 2.5 for Thermo Scientific Proteome Discoverer 2.5 software
12. Thermo Fisher Scientific: How to rely on productivity of your HPLC system during a pandemic
13. LCGC/Agilent Technologies: Poly- and Perfluoroalkyl Substances in Water: Challenges and Mitigating Strategies in Detection and Treatment
14. Shimadzu Scientific Instruments: Shimadzu&#39;s LC Method Development (RP) Series - Session 2: Optimising your Method
15. LCGC/Agilent Technologies: Identification, Mapping and Relative Quantitation of SARS-CoV-2 Spike Glycopeptides by Mass-Retention Time Fingerprinting
16. Thermo Fisher Scientific: FreeStyle: RAW Data Browsing and Analytics Software, and Almanac Instrument Monitoring and Management software
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LC and LCMS webinars - Week 49

N-nitrosamines in 21th Century

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3. SelectScience/Waters Corporation: Non-specific adsorption: Common causes, how it affects chromatographic results, and how to prevent it
4. Technology Networks/Thermo Fisher Scientific: Native Mass Spectrometry and COVID-19: Uncovering Drug Binding and Oligomerization 
5. Bruker Daltonics: Classification of Invasive Melanoma by Imaging Mass Spectrometry
6. Thermo Fisher Scientific: Increased confidence in peptide identification and quantification using Thermo Scientific Proteome Discoverer 2.5 software and INFERYS Rescoring
7. Agilent Technologies: Quantification of Organophosphorus Nerve Agent Metabolites (OPNA) on the 6495C
8. Agilent Technologies: Next Generation Bio LC Solutions: Workflow solutions with the new 1290 Infinity II Bio LC System with High-Speed Pump
9. Separation Science/Agilent Technologies: Super Charge your Method Development with a Quick, Easy Universally Compatible LC and LC/MS Method
10. LCGC/Agilent Technologies: LC/MS/MS Workflow to Determine over 500 Pesticides Residues in Compliance with SANTE 12682/2019 Guidelines
11. Technology Networks/SCIEX: Analysis of Process Related Impurities in Cell &amp; Gene Therapy Products by SWATH LC-MS
12. SelectScience/Phenomenex: Sample Preparation Strategies for Oligonucleotides Bioanalytical Workflows
13. Agilent Technologies: WHAT Do I Need for Successful Method Development?
14. Agilent Technologies: Understanding the Language of Liquid Chromatographic Results
15. LCGC/Agilent Technologies: The benefits of dedicated HPLC and chromatography column for analysis of Biomolecules
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LC and LCMS webinars - Week 50

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3. Agilent Technologies: OpenLab CDS Introductory Demonstration
4. Technology Networks/Agilent Technologies: Debunked: The Truths Behind Pesticide Testing
5. Agilent Technologies: Seminar 1 – OpenLab CDS - The Control Panel and Data Acquisition
6. Phenomenex: Benefits of Phenyl Stationary Phase Chemistries in HPLC
7. SelectScience/Waters Corporation: Taming the Viral Beast: Applying LC and LC-MS Technologies to the Analysis of rAAV Viral Vectors
8. C&amp;EN/Thermo Fisher Scientific: Comprehensive solutions to Nitrosamine impurity analysis: an industry case study
9. Bruker Daltonics: Moving Away From Immunoassays: Adapting LC-MS for Routine Urine Toxicology Screens
10. Agilent Technologies: Dual Laser Scattering Use in the Characterization of Proteins including mAbs
11. Shimadzu Scientific Instruments: Shimadzu&#39;s LC Method Development (RP) Series - Session 3: Approach to Rectifying Changes in Data
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Vědecký recenzovaný online časopis Kvasný průmysl (eISSN 2570-8619), vychází jako samostatné periodikum v angličtině. Od ročníku 2015 je obsah indexovaný databází Web of Science (edice ESCI), dále je indexován v databázích CAS, CAB Abstracts, DOAJ.
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LC and LCMS webinars - Week 51

LC and LCMS webinars - Week 50

N-nitrosamines in 21th Century

LC and LCMS webinars - Week 49

LC and LCMS webinars - Week 48

LC and LCMS webinars - Week 47

LC and LCMS webinars - Week 46

LC and LCMS webinars - Week 45

Hops - an abundant source of antioxidants. Methods to assessment of antioxidant activity of hop matrix

LC and LCMS webinars - Week 44

Upcoming events

Restek at EPRW 2026

16th European Pesticide Residue Workshop (EPRW 2026)

Thermo Fisher Scientific at HPLC 2026

Webinars

Biopharma fundamentals: Fragment analysis by CE

Proteome Discoverer 3.3 SP1: Processing DIA data with DIA-NN node

Improving RNA Purity and Measurement for Reliable Bioprocessing