🎤Alex BrodyIn this episode of Concentrating on Chromatography, we sit down with Alex Brody, an organic chemist at Aqua America, to discuss the rigorous analytical methods required for regulated drinking water testing.Alex walks us through the multi-step PFAS detection process using EPA Method 537.1, including extraction, nitrogen blowdown concentration, and LC-MS analysis. He explains why sample preparation and quality control are critical for achieving trace-level detection—and why these methods can't be rushed or simplified, even with new technologies available.We also explore taste and odor investigations, disinfection byproducts, and volatile organic compounds, plus the surprising reality of why regulated labs move slowly when adopting new instrumentation. You'll learn about the quality control checkpoints, peer review processes, and the misconceptions surrounding analytical turnaround times in compliance labs.<h5>Topics Covered:</h5><ul><li>Role of organic chemistry in regulated drinking water labs</li><li>EPA compliance requirements and regulatory bodies (EPA, PA-DEP)</li><li>PFAS detection using Method 537.1 and LC-MS</li><li>The critical importance of nitrogen blowdown sample concentration</li><li>Taste and odor analysis: MIB, Geosmin, and unknown compound identification</li><li>Why regulated methods evolve slowly (validation timelines, approval processes)</li><li>Quality control procedures: calibration checks, matrix spikes, surrogate standards, internal standards</li><li>Automated sample preparation and lab efficiency</li><li>Future PFAS regulations and Method 533 expansion</li></ul><h5>Ideal For:</h5><ul><li>Analytical chemists and environmental lab professionals</li><li>Water utilities and compliance officers</li><li>Chromatography practitioners interested in regulated workflows</li><li>Anyone curious about how drinking water safety is ensured</li></ul><h2>Video Transcription</h2><h3>Ensuring Safe Drinking Water Through Advanced Analytical Chemistry</h3><h4>Insights from a Regulated Water Testing Laboratory</h4>Analytical laboratories responsible for regulated drinking water testing operate under strict compliance frameworks to ensure the delivery of safe and reliable water supplies. In this interview, Alex, an organic chemist at Aqua America, outlines the scope of analytical work conducted in a modern water quality laboratory. The team primarily focuses on the determination of taste- and odor-causing compounds, disinfection byproducts, volatile organic compounds (VOCs), and per- and polyfluoroalkyl substances (PFAS). These analyses rely heavily on GC-MS and LC-MS instrumentation, supported by rigorous maintenance programs to ensure continuous operational performance and data reliability.<h4>Regulatory Compliance and Quality Control in Drinking Water Analysis</h4>Operating as a regulated drinking water laboratory requires adherence to stringent requirements established by agencies such as the U.S. Environmental Protection Agency (EPA) and state-level regulatory bodies. Compliance is driven by extensive quality control protocols integrated into every analytical batch. Typical workflows include calibration verification, matrix spike recovery assessments, internal standard monitoring, and surrogate performance checks. Notably, analytical batches are often limited in sample number to accommodate a substantial proportion of quality control samples, reflecting the critical importance of data defensibility in regulatory reporting.One common misconception is the perceived speed at which results can be delivered. In reality, comprehensive sample preparation, including extraction procedures and validation steps, can extend turnaround times to a full working day or longer. Additionally, validated regulatory workflows impose limitations on the rapid adoption of new analytical technologies, as method modifications must undergo formal evaluation and approval before implementation.<h4>Analytical Strategies for Taste and Odor Investigations</h4>Taste and odor events represent a significant operational challenge for water utilities. Standard targeted methods are routinely applied for compounds such as 2-methylisoborneol (MIB) and geosmin, which are commonly associated with earthy or musty sensory characteristics. When targeted analysis does not reveal the source, laboratories employ broad-scan GC-MS screening combined with spectral library interpretation to identify potential causative compounds. In some cases, sensory evaluation panels complement instrumental analysis, providing preliminary insight into potential treatment-related or environmental influences affecting water quality.<h4>PFAS Monitoring: Methodological Complexity and Trace-Level Sensitivity</h4>PFAS determination represents a rapidly evolving priority in drinking water monitoring. The laboratory currently applies EPA Method 537.1, with efforts underway to expand capabilities through certification for Method 533, thereby broadening analyte coverage. Analytical workflows typically involve solid-phase extraction followed by solvent evaporation using nitrogen-assisted blow-down techniques to achieve the trace-level sensitivity required for regulatory compliance. This concentration step is essential because PFAS compounds remain non-volatile under controlled evaporation conditions, enabling enhanced detection performance upon reconstitution and LC-MS analysis.<h4>Future Directions in Drinking Water Analytics</h4>Looking ahead, the primary technological driver in regulated water analysis is the demand for increasingly lower detection limits and expanded compound coverage. While regulatory frameworks can slow methodological innovation, laboratories continue to pursue efficiency improvements, such as automated sample preparation systems and optimized analytical workflows. These advances support the growing need to process high sample volumes while maintaining the stringent data quality standards required for public health protection.Ultimately, the work performed in regulated drinking water laboratories represents a critical intersection of analytical chemistry, environmental monitoring, and public safety. Continuous methodological refinement, coupled with advances in mass spectrometry and sample preparation technologies, will play a central role in addressing emerging contaminants and ensuring long-term water quality assurance.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Regulated Drinking Water Testing: PFAS Detection, Sample Prep & Real Lab Insights

This study investigates how ionization polarity influences the gas-phase stability of native-like proteins analyzed by ion mobility spectrometry–mass spectrometry. While proteins generally retain folded structures in both positive and negative electrospray ionization modes, significant differences in unfolding behavior were observed.Proteins exhibited greater gas-phase stability when analyzed in the ionization polarity corresponding to their solution-phase charge, requiring higher energy for unfolding. These findings emphasize the importance of considering protein isoelectric points and ionization polarity in native IMS-MS experiments to ensure accurate structural characterization.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295">Investigating the Effect of Isoelectric Points on the Gas-Phase Stability of Native-like Proteins Analyzed in Positive- versus Negative-Ion Mode by IMS-MS</a></h4>Alexis N. Edwards, Madeline G. Bannon, Michael S. Cordes, and Elyssia S. Gallagher*Anal. Chem. 2026, 98, 6, 4518–4527<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.analchem.5c04295">https://doi.org/10.1021/acs.analchem.5c04295</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.<div>In native mass spectrometry (nMS), the native-like structures of proteins are maintained in the gas phase by preserving the secondary, tertiary, and quaternary structures. <a href="javascript:void(0);">(1−7)</a> The preservation of native-like protein structures is primarily accomplished via the use of electrospray ionization (ESI), a soft ionization method that forms a distribution of multiply charged protein ions. These multiply charged ions can then be investigated to characterize stoichiometry, oligomeric composition, and ligand binding. <a href="javascript:void(0);">(8−10)</a>During ESI, a potential is applied to the sample-containing solution. In an aqueous spray solvent, H3O+ and OH– are produced in positive-ion mode (+ESI) and negative-ion mode (−ESI), respectively. <a href="javascript:void(0);">(11)</a> The buildup of like-charges within the solution results in the formation of a Taylor Cone at the capillary tip, which ejects charged droplets that travel toward the inlet of the mass spectrometer. Droplets undergo evaporation and fission until complete analyte desolvation, leading to the release of folded, gas-phase protein ions. As the droplets shrink, H3O+ or OH– can become concentrated, causing the pH of the droplet to decrease or increase, respectively. Shifts in pH can denature proteins. Therefore, to maintain native-like protein structures, nMS is conducted with solutions containing volatile salts, usually ammonium acetate, that provide ionic strength and minimize pH changes compared to spraying from pure water. <a href="javascript:void(0);">(11,12)</a> Droplet pH in ESI is influenced by flow rate, solvent composition, and applied voltage, <a href="javascript:void(0);">(13)</a> but often approaches values near the pKa of acetate (4.76) or ammonium (9.25) in +ESI or −ESI, <a href="javascript:void(0);">(12,14)</a> respectively. During ESI, changes in overall protein charge take place via proton transfer at chargeable amino acid residues, resulting in the formation of protonated or deprotonated protein ions with the charged sites being dependent on the relative proton affinities of ammonium, acetate, and chargeable amino acids as proteins near complete desolvation. <a href="javascript:void(0);">(15−19)</a>Ion mobility spectrometry (IMS) measures an ion’s mobility through an inert gas under the influence of an electric field. Thus, mobility is directly influenced by the ion’s size, shape, and charge. <a href="javascript:void(0);">(20)</a> A protein’s mobility can be used to estimate its structure by calculating an average momentum-transfer collisional cross section (CCS). In addition to structural characterization, IMS-MS can investigate the gas-phase stability of protein complexes when paired with collision-induced unfolding (CIU). Specifically, in CIU experiments, protein ions are accelerated into a cell filled with inert gas molecules and undergo multiple collisions with higher frequency and energy relative to IMS. This converts kinetic energy to internal, vibrational energy, disrupting noncovalent interactions and inducing unfolding of the protein ions. <a href="javascript:void(0);">(21)</a> Unfolding alters the protein structure, which can then be quantified by monitoring the protein’s mobility during subsequent IMS-MS measurement. <a href="javascript:void(0);">(22)</a> Prior work with mutant proteins has shown that proteins with similar intramolecular interactions have similar CIU fingerprints, while proteins with different intramolecular interactions have different CIU fingerprints. <a href="javascript:void(0);">(23)</a> Therefore, gas-phase protein stability is correlated with intramolecular interactions.Past native IMS-MS work has primarily been conducted in +ESI given that -ESI has the reputation of being unreliable for native-like protein analysis due to the occurrence of corona discharge, <a href="javascript:void(0);">(24)</a> and charge-state distributions that may not be representative of folded proteins. <a href="javascript:void(0);">(25)</a> In recent years, −ESI has been increasingly used in CIU and IMS-MS, with recent work demonstrating that −ESI-MS is capable of discerning denatured and native-like protein structures. <a href="javascript:void(0);">(26,27)</a> Prior work showed that charge manipulation of folded proteins, such as charge reduction, to achieve similar absolute charge states in both ion modes, results in CCS values of protein cations that are comparable to their anion counterparts. <a href="javascript:void(0);">(26,28)</a> This suggests that gas-phase structures are weakly dependent on protein charge and ionization polarity. <a href="javascript:void(0);">(23,26,28)</a> Yet, Hong et al. showed that, in the absence of charge reduction, the gas-phase stability of two proteins (avidin and β-lactoglobulin) is sensitive to ionization polarity, highlighting that gas-phase stability is not consistent between +ESI and −ESI. <a href="javascript:void(0);">(29)</a><h4>Materials and Methods</h4><h5>Native IMS-MS Analysis</h5>Glass capillaries were pulled using a P-1000 micropipette puller (Sutter Instrument Company, Novato, CA). Samples and calibrants were sprayed from pulled capillaries (inner diameter ∼1 μm) and analyzed with a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/152">Synapt G2-S High-Definition MS </a>(Waters Corporation, Milford, MA). The trap and transfer cells contained Ar, the helium cell contained He, and the IMS cell contained N2. IMS wave velocity was 350 m/s, while IMS wave height was either 7 or 9 V, depending on the protein size (see <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Table S2</a>). Proteins with larger mass required a wave height of 9 V to ensure that all the ions migrated out of the IMS cell prior to the next ion injection. Mass spectra were collected and signal averaged over 30 s. Additional instrument details can be found in the Supporting Information (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Table S2</a>).To limit systematic error due to repositioning of the ESI tip between runs, <a href="javascript:void(0);">(48)</a> the XYZ-stage position was optimized and kept consistent for all replicates. Approximately 5 μL of sample was loaded into a glass capillary, which was then mounted on the XYZ-stage. Electric potential was delivered to the sample by a platinum wire inserted into the capillary and placed in contact with the solution. The voltage applied to the tip holder ranged from 0.60 to 1.2 kV for native samples, and 0.80 to 1.40 kV for denatured samples. Nine replicates in each ion mode were collected for all proteins with measurements spread across 8 days over 3 weeks. These replicates were conducted in pairs by collecting data in both ion modes consecutively. To accomplish this, a CIU experiment was collected in +ESI before the instrument polarity was changed to −ESI. A second CIU experiment was performed, beginning with −ESI to counteract any changes in protein stability due to pH changes during ESI. <a href="javascript:void(0);">(14)</a> This allowed for use of the same capillary, at the same position in relation to the instrument inlet, for each +ESI/–ESI replicate pair. A new tip was used for each +ESI/–ESI replicate pair. CIU experiments were accomplished by increasing collision voltage (CV) to the trap cell in 5 V steps, from 0 V to a maximum of 120 V.<h4>Results and Discussion</h4>Ten proteins were chosen for analysis in +ESI and −ESI in this work (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#tbl1">Table 1</a>). These proteins can be broken into three groups depending on the net-charge in solution at pH 7: net-negative (with pI &lt; 6.5), net-neutral (with 6.5 ≤ pI ≤ 7.5), and net-positive (with pI &gt; 7.5). Five of the ten proteins have a net-negative charge at pH 7, though four of these proteins (con A, β-lactoglobulin, ovalbumin, and α-lactalbumin) are routinely analyzed via +ESI-IMS-MS. <a href="javascript:void(0);">(18,28)</a> Additionally, each protein had charge-state distributions in +ESI and −ESI that resulted in at least one overlapping charge state (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#fig1">Figure 1</a>), allowing for direct comparison of TWCCSN2→He and CIU50 in both ion modes.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2026_98_6_4518_4527_Figure_1_Representative_mass_spectra_in_ESI_top_and_ESI_bottom_cb81976247.jpg" alt="Anal. Chem. 2026, 98, 6, 4518–4527: Figure 1. Representative mass spectra in +ESI (top) and −ESI (bottom) for (A) ovalbumin, (B) GNA, (C) β-lactoglobulin, (D) α-lactalbumin, (E) con A, (F) streptavidin, (G) ubiquitin, (H) WGA, (I) cytochrome c, and (J) lysozyme. Proteins that have blue or red charge states indicate additional oligomeric states that were detected. Outlines in red boxes are overlapping charge states that are compared herein. *Indicates contaminant peaks." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2026_98_6_4518_4527_Figure_1_Representative_mass_spectra_in_ESI_top_and_ESI_bottom_cb81976247.jpg 245w," sizes="100vw" width="1600" height="865"><h5>Proteins with Disulfide Bridges are Stable in Both Polarities</h5>For both lysozyme and α-lactalbumin, the average TWCCSN2→He were within experimental uncertainty in +ESI and −ESI for both the initial, folded structures and the final, extended structures (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#tbl2">Table 2</a>). However, the 6+ and 6- ions of lysozyme and α-lactalbumin showed a high resistance to unfolding with &gt;90% of the ions retaining their folded TWCCSN2→He values even at high CV (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#fig2">Figure 2</a>). Replicate data showed similar trends (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Figure S2</a>). While CCS values provide a description of gas-phase structures, CIU experiments provide information on the stability of the gas-phase protein structures. Furthermore, the number and pattern of disulfide bridges present in antibodies has been characterized by CIU. <a href="javascript:void(0);">(63)</a> Lysozyme and α-lactalbumin both contain the glycosyl hydrolases family 22 (GH22) domain, which has four disulfide bridges that span ∼94% and ∼93% of the amino acid sequence, respectively (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Figure S3</a>). Thus, we hypothesize that rather than effects from protein pI and ionization polarity, the unfolding behavior of these proteins is controlled to a greater extent by the presence of stabilizing disulfide bridges that prevent routine unfolding during collisional activation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2026_98_6_4518_4527_Figure_2_Representative_mobiligrams_showing_arrival_times_for_lysozyme_A_C_and_a_lactalbumin_B_D_0f3d23c3c1.jpg" alt="Anal. Chem. 2026, 98, 6, 4518–4527 - Figure 2. Representative mobiligrams showing arrival times for lysozyme (A, C) and α-lactalbumin (B, D) at low and high CV (blue solid and orange dashed lines, respectively) for +ESI (A, B) and −ESI (C, D). For lysozyme, different values were chosen for the high CV (e.g., 540 eV in +ESI and 480 eV in −ESI) because these were the last analyzed CV where the signal-to-noise ratio (S/N) for the 6 ± ions was greater than 3." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2026_98_6_4518_4527_Figure_2_Representative_mobiligrams_showing_arrival_times_for_lysozyme_A_C_and_a_lactalbumin_B_D_0f3d23c3c1.jpg 212w," sizes="100vw" width="1600" height="1178">To characterize the stabilizing effect of disulfides, MD simulations were conducted that heat denatured 6+ ions of lysozyme and α-lactalbumin with disulfide bridges either present or removed with the resulting cysteine residues being protonated prior to heating (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Figure S4</a>). Structural changes associated with heat denaturation were characterized by taking the root-mean-square deviation (RMSD) of the heated structures relative to initial, crystal structures. Over ten trials, lysozyme ions possessed an average RMSD of (1.6 ± 0.2) nm when disulfides were present which increased to (3.5 ± 0.5) nm when disulfides were removed. Similarly, the RMSD of α-lactalbumin ions increased from (1.8 ± 0.1) nm when disulfides were present to (3.7 ± 0.3) nm when disulfides were removed, suggesting that the disulfides serve as a barrier to unfolding. This is visually represented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Figure S4C,D</a> where simulated structures of heated lysozyme and α-lactalbumin ions with intact disulfides adopt more condensed configurations relative to those with disulfides removed. In essence, the disulfides “tie” disparate regions of protein together, which serves as a barrier to more thorough unfolding even at a maximum simulated temperature of 900 K, explaining the similarity of TWCCSN2→He values in +ESI versus −ESI as well as the limited percentage of ions that undergo a change in TWCCSN2→He upon additional collisional activation.WGA has 16 disulfide bridges per monomeric subunit (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#tbl1">Table 1</a>). On average, these disulfides encompass seven amino acids each, with ∼78% the total amino acids in WGA being encompassed by at least one disulfide bridge (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Figure S3</a>). Compared to lysozyme and α-lactalbumin, the presence of disulfide bridges across small numbers of amino acids enables WGA to unfold with ΔCCS of (370 ± 60) Å2 and (390 ± 120) Å2 in +ESI and −ESI, respectively (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#tbl2">Table 2</a>). However, in comparison to the other lectins, GNA and con A, we hypothesize that these disulfide bridges in WGA limit the extent of unfolding, resulting in the extended, TWCCSN2→He values being within experimental uncertainty in both ion modes.To summarize, lysozyme and α-lactalbumin exhibit similar gas-phase behavior in both +ESI and −ESI, with most ions retaining folded structures even under high CV. We hypothesize this resistance to unfolding is largely attributed to their four disulfide bridges, which span over 90% of their sequences and act as stabilizing constraints. MD simulations confirm that removing these disulfides significantly increases structural deviation during heat denaturation, indicating their critical role in maintaining compact configurations. Consequently, the presence of disulfide bridges explains the minimal changes in TWCCSN2→He and the proteins’ high stability under activation.<h4>Conclusions</h4>IMS-MS allows for detailed analysis of the structure and stability of native-like proteins in the gas phase. The structure of a protein is dictated by its amino acid sequence and the resulting noncovalent interactions that form the secondary, tertiary, and quaternary structures. Chargeable amino acid residues that are solvent exposed can carry charges leading to the protein having a net-positive, net-negative, or net-neutral charge in solution at physiological pH. Yet, most native IMS-MS analysis is conducted in +ESI, leading to proteins that are net-negative in solution experiencing large swings in their net-charge during the ESI process.This work highlights that gas-phase stability of native-like proteins varies with ionization polarity. While CCS values for folded structures are generally comparable between +ESI and −ESI, unfolding energetics differ─net-negative proteins are more stable in −ESI, whereas net-positive proteins are more stable in +ESI. We hypothesize that these differences are due to changes in the intramolecular interactions within cationic and anionic proteins. Therefore, proteins analyzed in the mode opposite to their solution-phase charge may have different gas-phase structures compared to biological systems. For example, proteins that are net-negatively charged in physiological environments (pI &lt; 6.5) are more likely to represent their biological counterparts as gas-phase anions when analyzed in −ESI compared to +ESI, enabling nMS experiments to more closely characterize the structures present in biological systems.</div>

Investigating the Effect of Isoelectric Points on the Gas-Phase Stability of Native-like Proteins Analyzed in Positive- versus Negative-Ion Mode by IMS-MS

Anna Vanluchene&nbsp;from UCT Prague’s&nbsp;Department of Chemical Engineering has received a&nbsp;prestigious start-up grant from the Dagmar Procházková Fund. However, she did not receive funding right away. In this interview, she describes how she developed ten versions of her&nbsp;Illuminating Flow&nbsp;project idea (combining photocatalysis with flow reactors for pharmaceutical synthesis). She explains why she considers perseverance to be crucial when applying for grants, how international experience has influenced her approach to team management, and how her research can reduce the chemical industry’s&nbsp;dependence on fossil fuels and imports from China.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_Na_startovaci_grant_jsem_cekala_dva_roky_a_napsala_deset_verzi_projektu_2_5b7d63b8e9.jpg" alt="VŠCHT Prague/Tomáš Balický: I waited two years for a start-up grant and wrote ten versions of my project idea" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_Na_startovaci_grant_jsem_cekala_dva_roky_a_napsala_deset_verzi_projektu_2_5b7d63b8e9.jpg 127w," sizes="100vw" width="1128" height="1387"><h5>What did the Dagmar Procházková Fund grant mean to you?</h5>I&nbsp;consider it to be a&nbsp;great success. It is the first injection of funding that will allow me to ensure full independence for my research endeavour, to start my own research group, and to fund students so I&nbsp;can get started properly.<h5>What have been your previous experiences with grants?</h5>During my doctorate, a cotutelle (international doctorate under dual supervision), I&nbsp;was funded by a&nbsp;grant from the French government. Then I&nbsp;worked at the Academy of Sciences of the Czech Republic (CAS) and received a&nbsp;Marie Skłodowska-Curie Action (MSCA) Postdoctoral Fellowship to go abroad. The idea for my now-funded project was developed about two years ago and, since then, I&nbsp;have gradually developed and adapted the idea for various grant calls. I&nbsp;submitted about ten different versions of the proposal. I&nbsp;did not receive a&nbsp;grant from the Dagmar Procházková Fund last year, but this year, my application was successful.<h5>This probably requires a&nbsp;great deal of patience and perseverance.</h5>Certainly, because the success rates for most grants have been declining for some time. But as a&nbsp;motivational example, I&nbsp;will tell the story of Timothy Noel, a&nbsp;Belgian professor of chemistry living in the Netherlands. He applied for the prestigious European Research Council (ERC) grant, which is among the most sought-after in Europe, seven times. But no failure deterred him and eventually he succeeded and opened the door to potentially massive grant funding. He was already one of the world’s&nbsp;top scientists in the field at that time and openly described his journey on his blog <a target="_blank" rel="noopener noreferrer" href="https://www.noelresearchgroup.com/2022/03/17/my-personal-story-to-obtain-an-erc-grant-7-submissions-4-interviews-and-6-heartbreaks/">blog</a>.<h5>What advice does this convey to other grant applicants and early career researchers?</h5>Start early, read a&nbsp;lot, don’t&nbsp;give up, and—most importantly—apply repeatedly. Learn from rejections and overcome possible frustration from failures. Read blogs and books by successful people, where you will find not only the glorious results of their work, but also the hardships they faced over their entire journeys. Of course, building your team and laboratory is impossible without finances. I&nbsp;myself still hope that I&nbsp;will be able to receive even bigger grants in the future.<h5>How has your international experience influenced your scientific approach?</h5>A&nbsp;lot and on several levels. Different laboratories teach you how to do&nbsp;things differently. You gain varied experience about how different institutions function. You see other people’s&nbsp;positive motivations, their ability to be ambitious without negativity, their involvement in institutional matters, their openness to cooperation with companies (e.g., tech transfers, spin-offs). The most important thing is to broaden your horizons. I&nbsp;see going abroad as a&nbsp;necessity. What I&nbsp;have taken away the most is drive, great support, and the idea that scientific output is not just about metrics, but also about dedication to one’s&nbsp;topic.<h5>What is your Illuminating Flow: Transport Phenomena in Photocatalysis about, in layperson’s&nbsp;terms, and how is it unique?</h5>Most of today’s&nbsp;chemical industry is still based on thermal processes and fossil fuels. Photochemistry enables electrification, where the energy source is photons delivered by LEDs, which can generate electricity from renewable sources. Current research focuses mainly on chemical reactions and their mechanisms, but as a&nbsp;chemical engineer, I&nbsp;focus on designing processes and reactors suitable for industrial use. In batch reactors, light fades rapidly with distance from a&nbsp;source, so much of a&nbsp;reaction mixture is not illuminated. Flow reactors solve this problem, but they bring new challenges such as working with solid catalysts and subsequently scaling up production and achieving higher production rates of the desired chemicals at a&nbsp;given scale.One can imagine a&nbsp;transparent tube with a&nbsp;catalyst on the walls, upon which light shines and through which reactants flow. This project has a&nbsp;unique emphasis on engineering solutions and overall process efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_Na_startovaci_grant_jsem_cekala_dva_roky_a_napsala_deset_verzi_projektu_3_b978abf25c.jpg" alt="VŠCHT Prague/Tomáš Balický: I waited two years for a start-up grant and wrote ten versions of my project idea" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_Na_startovaci_grant_jsem_cekala_dva_roky_a_napsala_deset_verzi_projektu_3_b978abf25c.jpg 241w," sizes="100vw" width="1228" height="799"><h5>So, were you inspired by a&nbsp;gap in the market to connect photocatalysis with transport phenomena?</h5>Exactly. I&nbsp;combine my areas of expertise: microfluidics, photocatalytic degradation of organic substances (CAS), and synthesis (MSCA Postdoctoral Fellowship). The idea itself is not new, and pharmaceutical companies are very interested in “greener” photochemistry, but in practice, there is often a&nbsp;lack of appropriate engineering solutions and apparatus for industrial use.<h5>What are the practical applications of your work and what substances are you focusing on?</h5>Mainly on pharmaceuticals and on the synthesis of so-called fine chemicals, which are often the building blocks for the production of pharmaceuticals. There is also a&nbsp;geopolitical dimension to this, i.e. less dependence on imports from China and sustainable synthesis in Europe where it makes economic sense.<h5>And what chemicals are you specifically talking about?</h5>For example, building blocks for advanced synthesis, compounds prepared by trifluoromethylation reactions, or products of oxidation reactions with singlet oxygen. At the same time, we focus on reactions that are, in the classical design process, less safe or technologically demanding, where new approaches, such as photochemistry in flow reactors, could be safer and have better process control. Of course, this is not a&nbsp;universal solution for all types of reactions.<h5>The goal of this project?</h5>I&nbsp;plan to start a&nbsp;fully functional laboratory and research team, create high-quality experimental data on selected molecules and based on them, try to build cooperation with industrial partners. Without data, it is impossible to come to companies with just an idea. Within three years, I&nbsp;would like to have results that can be realistically presented and further developed.<h5>What are the main challenges in transport phenomena during photocatalysis?</h5>The methodology for repeatability of experiments in photochemistry is relatively demanding and there are unforeseen obstacles in building apparatus. Also, the scale-up from microliters to a&nbsp;larger scale.<h5>What methodology will you use, and how big are the microreactors and their flow rates?</h5>I&nbsp;have two different directions in methodological design. The first direction involves microreactors the size of a&nbsp;laboratory slide. You make a&nbsp;structure using a&nbsp;laser, and then you have to cast it and make a&nbsp;microreactor. This allows you to quickly adjust the geometry of the reactor. This method is, of course, not applicable in the commercial setting, but it is ideal for studying transport phenomena and precise measurements.The second direction involves reactors that are flow cells. These are already larger, approximately 15 x 15 centimetres, and their flow rates are in the order of millilitres per hour. This is the initial scale that we will further increase during the project.<h5>There is a&nbsp;big difference between a&nbsp;flow rate of microlitres per hour and industrial needs. Will it not work differently in reality?</h5>Our research will focus on studying the phenomena themselves for now, and scaling will be addressed later. In addition, we will combine the experimental data obtained with computer simulations that will help design suitable conditions for a&nbsp;larger scale. For some high-value fine chemicals, these scales, combined with continuous operation or parallelization of reactors, may already be practically usable.<h5>What catalysts will you use and how will you attach them to the reactor walls?</h5>I&nbsp;am focusing on heterogeneous photocatalysis, and we will mainly use graphitic carbon nitride and its more advanced modifications. These are semiconductor materials that can be synthesized relatively easily and are suitable for immobilization on glass reactor walls. A&nbsp;key part of this project is the creation of a&nbsp;stable catalytic layer for long-term flow-through operations. To increase the contact of reactants with the catalyst, we will also consider multiphase modes, for example, gas bubbling, in order to disrupt the otherwise laminar flow.<h5>Why did you choose this field and the Department of Chemical Engineering?</h5>During my postdoc in Belgium, the department had an opening for an Assistant Professor position that caught my attention and I&nbsp;applied for it. I&nbsp;did my undergraduate, graduate, and initial doctoral studies here, and I&nbsp;love the department. I&nbsp;have a&nbsp;Bachelor’s&nbsp;degree in Chemistry.<h5>Are you collaborating with anyone?</h5>We collaborate with BUT Brno focusing on creating layers and immobilizing catalysts on glass, as well as with the Institute of Chemical Process Fundamentals of the Academy of Sciences of the Czech Republic. And as for organic synthesis, we are connected with Ghent University via postdoc internships. At the same time, my colleague Viola Tokárová and I&nbsp;received a&nbsp;Czech Science Foundation grant in cooperation with BUT Brno and Masaryk University, with our part of the project focusing on the use of flow reactors for photochemical reactions and testing the antibacterial properties of photocatalytic materials. And I&nbsp;am writing other grant proposals in which I&nbsp;am trying to build new collaborations.<h5>What does the creation of an independent research group at UCT Prague look like?</h5>I&nbsp;started by renovating the premises that were assigned to me and gradually building my team. It is important for research work to have Ph.D. students; I&nbsp;have already hired one and we will recruit more. In addition to building the group, I&nbsp;will engage in securing further funding for the group, so I&nbsp;will be writing more grant proposals. It is important to think far enough in advance when it comes to prospective funding for a&nbsp;research group.<h5>What type of students are you looking for?</h5>The laboratory and team I&nbsp;are building will be very multidisciplinary, just like the area I&nbsp;am working on. That is why I&nbsp;am looking for students who are attracted to true multidisciplinary research, those who, for example, have knowledge of engineering, but who don’t&nbsp;mind a&nbsp;little bit of pure chemistry. One student in their own right does not have to (and cannot) know every subject, so I&nbsp;will ideally have a&nbsp;team consisting of different people with different research backgrounds. Motivated, DIY-type students who would rather enthusiastically build or invent something themselves than conduct repetitive measurements. Organic or analytical chemists will also find their place here. If this topic is interesting to students in any way, they can definitely contact me. I&nbsp;am also open to interdepartmental cooperation with other UCT Prague Faculties.<h5>How would you describe your path to photocatalysis/chemical engineering?</h5>Studying Chemistry as an undergrad gave me a&nbsp;broad perspective, but I&nbsp;was gradually narrowing my focus and eventually chose engineering. Chemical engineering intrigued me with its logic and was the easiest for me to grasp at the time. But I&nbsp;have always enjoyed working on projects more than the studies themselves. Even during my Bachelor studies, I&nbsp;really enjoyed and was interested in the Professor Štěpánek Group’s&nbsp;projects, where I&nbsp;was doing something completely different than I&nbsp;do&nbsp;now. I&nbsp;only got into photochemistry during my time at CAS and then, during my postdoctoral fellowship, I&nbsp;devoted myself to electrochemistry. My current focus is, thus, a&nbsp;natural interlinking of all these areas. Consequentially, I&nbsp;developed an awareness of the necessity to become independent and return to the department with my own, different research agenda, which, in my opinion, is an important and correct basis for establishing my own research group.<h5>What is the biggest challenge of this project for you?</h5>The transition from being a&nbsp;postdoc to a&nbsp;leadership position is a&nbsp;big change for me and I&nbsp;think it is not talked about enough. It means a&nbsp;shift from my own experimental work to leading a&nbsp;team, planning and delegating tasks, and motivating students. From a&nbsp;practical point of view, one of the main challenges is the transfer of reactions from the microscale to functional systems, the construction of reactors, the immobilization of catalysts, and generally confirming that this direction makes sense in the long term and that it has future potential.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Praha_Na_startovaci_grant_jsem_cekala_dva_roky_a_napsala_deset_verzi_projektu_1_66ac267e06.jpg" alt="VŠCHT Prague/Tomáš Balický: I waited two years for a start-up grant and wrote ten versions of my project idea" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_VSCHT_Praha_Na_startovaci_grant_jsem_cekala_dva_roky_a_napsala_deset_verzi_projektu_1_66ac267e06.jpg 155w," sizes="100vw" width="830" height="839">

I waited two years for a start-up grant and wrote ten versions of my project idea

Article

Video

Scientists

Water

Scientific article

Interview

Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

ECOM PREPSYSTEM - Chromatography Separation System

A complete flash/preparative/pilot scale/production modular system ready for customizable to the application needs.

We would like to introduce an ECOM product news – a complete flash/preparative/pilot scale/production modular system with an excellent price/performance ratio.<h3>Applications</h3>The system can be used e.g. in:<ul><li>high-capacity systems or with centrifugal liquid/liquid chromatography systems, like in pharmaceutical purification of plant extracts – e.g. cannabinoids (CBD, CBN, CBG, CBC, THC, etc.), peptides, and oligonucleotides, algae extracts, oleoresins, etc.,&nbsp;</li><li>Also in new molecule development or synthesis.</li></ul><h3>Main parts</h3><h4>Pumps</h4><ul><li>The device has 3 pumps, 2 main ones are intended for a "high" pressure gradient (parallel pump connection), and one of them has a 6-solvent selection valve.&nbsp;</li><li>An optional third pump is used for repetitive sampling of high-volume samples. The unit also has a loop sample injection valve and a switching valve for CCC/CPC applications (ascending/descending mode).&nbsp;</li><li>The unit's universal PDA detector measures absorbance at four wavelengths at the same time up to 800 nm.</li></ul><h4>Software</h4><ul><li>The ECOMAC software or <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a> (FDA 21 CFR Part 11 compliant) is internally installed in the PC controlling the system.</li></ul><h4>Customization</h4><ul><li>The unit is completely customizable to the application needs to meet any scale criteria–from small flash sample separation testing through pilot scale prediction to high-capacity production.</li><li>It could work in ranges of 10 ml/min.–6000 ml/min and it can be easily and automatically switched between these scales.</li></ul>

ECOM ECS23PC Quaternary Preparative Gradient System with Fraction Collector

A highly effective preparative system containing a UV-VIS DAD detector, a preparative pump, a gradient box with PC, and a fraction collector. The detector allows measuring at four wavelengths at the same time or an informative scan.

<div style="display:block;" aria-labelledby="tab_item-0">A highly effective preparative system containing a UV-VIS DAD detector, a preparative pump, a gradient box with PC, and a fraction collector. The detector allows measuring at four wavelengths at the same time or an informative scan.Communication is through RS232 or Ethernet (LAN). An additional PC A/D converter card is not required for this system. The system contains a manual injector and ECOMAC software which include drivers for all ECOM units.It is necessary to purchase an OS for the system - in most cases, we recommend OEM Windows OS (P/N: ABB90000) or Linux (on request). It is also possible to purchase a wireless mouse and keyboard set (P/N: EPC-0028) and a computer monitor (P/N: EPC-0027).<h3>Main parts</h3><h4>TOY18DAD H 600 DETECTOR</h4>A UV-VIS diode array detector, which allows measuring the absorbance of four wavelengths in the range of 200 – 600 nm simultaneously in one cell just as visualization of whole spectra. It is possible to use the detector in flash and preparative applications. Standardly is assembled preparative cell PLCC 15 but other cells are available.<h4>ECP2300 PREPARATIVE PUMP</h4>This isocratic pump works with Gradient Box ECB2007PC as a quaternary gradient pump. The flow rate range is 0.1-300 ml/min and the pressure limit at 15 MPa. Unit software includes a new learning algorithm for pulsation suppression and many testing and diagnostic functions.<h4>ECB2005PC GRADIENT BOX WITH PC, WITHOUT DEGASSER</h4>The box is suited for handling liquids in ECOM gradient systems. It accommodates a container for solvent bottles and a four-way gradient valve. A four-way gradient valve is controlled from the pump ECP2300. In this box is assembled an industrial PC board. This solution allows the installation of OS (Windows or Linux) and chromatography SW ECOMAC or Clarity.&nbsp;<h4>ECF2096 FRACTION COLLECTOR</h4>Fractions are collected according to a chosen method which can be created manually by keyboard, just as using ECOMAC software. Analog input allows collecting based on detector signal. Racks for 9, 21, and 40 ml tubes.<h4>ECOMAC CHROMATOGRAPHY SOFTWARE</h4>This system is fully controlled by ECOMAC software, which is also a part of delivery. It allows all units control, gradient creation, and fraction collecting.<h3>SYSTEM CONTAINS&nbsp;</h3>Pcs / Description (P/N)<ul><li>1x / TOY18DAD H 600 Four Channel Detector (ANW0000X)</li><li>1x / ECB2005PC Gradient Box with PC, without Degasser (ABBPC00X)</li><li>1x / ECP2300 Preparative Pump (ACE0000X)</li><li>1x / ECOMAC software (SA100000)</li><li>1x / ECF2096 Fraction collector (AFA0000X)</li><li>1x / Preparative injection valve 1/8”; 2ml loop (SY2032022)</li><li>1x / Accessory for prep. system series 2000 (ASP00180)</li></ul>Note: The system can be equipped with detectors with different wavelengths ranges and pumps with different flow rates from ECOM production.</div>

ECOM ECP201LG Gradient Preparative Pump 1000 mL/min

An innovative, powerful, and cost-effective unit for preparative LC separations with high pressure. 4 low pressure gradient valves, flow up to 1000 mL/min. 

<div style="display:block;" aria-labelledby="tab_item-0">The ECP201L Preparative Pump is an innovative, powerful, and cost-effective unit for preparative liquid chromatography where high pressure is needed. The pump with 4 low-pressure valves delivers a flow rate of up to 1000 mL/min at a very high output pressure of up to 15 MPa (2176 psi).<h3>Main features and benefits</h3><h4>Durability</h4>The pump is constructed for high pressure and flow rates. This makes it suitable for day-to-day long-term use in production<h4>Wide application possibilities</h4>The unit is used for pharmaceutical separations and plant extract purification, such as cannabinoids, peptides, and oligonucleotides. Also, it has much wider use in other chemical production, such as dosing into chemical reactors, and in various laboratory uses. Another advantage is the usability of the same device for separation method development.<h4>Smart controls and diagnostics</h4>It is easy to operate, especially with ECOMAC and Clarity software. This makes unit management powerful and convenient. The basic version of the ECOMAC software is available free of charge. It is also used for service and diagnostics.<h4>Other useful features</h4><ul><li>A new learning algorithm for pulsation suppression</li><li>A leakage sensor</li><li>A seal wash kit for longer piston seal life</li><li>Also available in configurations made of certified bio-inert materials</li></ul><h3>Parameters</h3><ul><li>Flow rate: 1 – 1000 ml/min</li><li>Maximum operating pressure in the pump: 15 MPa up to 1000 ml/min</li><li>Flow rate setting: 1 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 1 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Wetted materials in the pump: stainless steel, PEEK, TefzelTM, Kalrez®, ceramic, seals GFP (PTFE) by default or UHMW-PE on request</li><li>Control: RS232, Ethernet(LAN), USB</li><li>Output capillary outer diameter: 1/8 "</li><li>Input tubing outer diameter: 3/8 ”</li><li>Number of gradient valves*: 4 (A,B,C,D)</li><li>Orifice: 5 mm</li><li>Fluid temperature: 5 - 90°C</li><li>Connecting thread: 5/8" - 18 UNF</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 500 VA</li><li>Weight of the complete set: 30 kg (66.14 lb)</li><li>Dimensions (w x h x d) of the complete set: 340 x 170 x 640 mm (13.4 x 6.7 x 25.2 in)</li></ul></div>

ECOM ECP2200 Preparative Pump

Powerful and durable pumps for preparative applications in low- and high-pressure liquid chromatography, designed with 2 pump heads connected in parallel

<div style="display:block;" aria-labelledby="tab_item-0">This pump works as an isocratic pump and together with a gradient box also as a gradient pump.These powerful and durable pumps are suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. They are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.&nbsp;Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. New material versions have been added-in, beyond to stainless steel A316L, also Hastelloy, titanium and PEEK for biocompatible applications. The pumps can operate in two modes, i.e. using constant pressure or using the newly added option of constant flow rate.&nbsp;<h4>Gradient function:&nbsp;</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2005, ECB2005PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals:&nbsp;</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support:&nbsp;</h4>The pumps are supported by ECOMAC and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a>.<h3>Features and Benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Heating unit for pump heads available</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).<h4>Accessories:</h4>Pump head heating unit, see the accessories. If you would like this function for your pump, add the heating unit.<h4>Parameters:</h4><ul><li>Flow rate: 0.001 – 250 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 psi) up to 100 ml/min; 15 MPa (2176 psi) at 250 ml/min</li><li>Flow rate setting: 0.001 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 1 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Communication: RS232,Ethernet (LAN),USB,Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 250VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 13.5 kg (30 lb)</li><li>Output capillary outer diameter: 1/8”</li><li>Upper pressure limit (MPa): 1– 30 (4351PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Pressure Compensation Learning: yes&nbsp;</li></ul></div>

ECP2050 Preparative Pump

Preparative HPLC pump with flow-rate ranges of 0.001 to 50 ml/min. 

<div style="display:block;" aria-labelledby="tab_item-0">This pump works as an isocratic pump and together with a gradient box also as a gradient pump.These powerful and durable pumps are suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. They are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. The pumps can operate in two modes, i.e. using constant pressure or the newly added option of constant flow rate. New material versions have been added-in beyond to stainless steel A316L, also Hastelloy, titanium and PEEK for biocompatible applications.<h4>Gradient function</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2007, ECB2007PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support</h4>The pumps are supported by ECOMAC and Clarity software.<h3>Main features and benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>&nbsp;Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Heating unit for pump heads available</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg" alt="ECOM: ECP2050 - Preparative Pump - Pressure Compensation Learning algorithm " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg 188w," sizes="100vw" width="700" height="580"><h3>Optional configurations/variants</h3>Pump head heating unit, see the accessories shown below. In case you would like this function for your pump, add also the heating unit to&nbsp;your cart. (Also can be found in the Accessories product category).<h5>PN / Wetted parts of pumping block</h5><ul><li>ACCH000X / Hastelloy</li><li>ACCP000X /<span style="-webkit-text-stroke-width:0px;display:inline !important;float:none;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;"> PEEK</li><li>ACC0000X / Stainless steel</li><li>ACCT000X / Titanium</li></ul><h4>Parameters:</h4><ul><li>Flow rate: 0.001 – 50 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 psi)</li><li>Flow rate setting: 0.1 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 0,5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Wetted materials: Stainless steel or Hastelloy or Titanium or PEEK + PEEK, TefzelTM, PE, ceramics, seals&nbsp;</li><li>Communication: RS232, Ethernet (LAN), USB, Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 250VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 12 kg (26,5 lb)</li><li>Output capillary outer diameter: 1/16”</li><li>Upper pressure limit (MPa): 0,1 – 30 (4351PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Pressure Compensation Learning: yes&nbsp;</li></ul></div>

ECOM ECP2100 Preparative Pump

 Preparative HPLC pump with a flow-rate range up to 100 ml/min. 

<div style="display:block;" aria-labelledby="tab_item-0">Innovated pumps ECP2100 work as isocratic pumps and together with a gradient box also as a gradient pump. These powerful and durable pumps are suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. They are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. New material versions have been added-in, beyond to stainless steel A316L, also Hastelloy, titanium and PEEK for biocompatible applications.The pumps can operate in two modes, i.e. using constant pressure or using the newly added option of constant flow rate. The ECP2100 pump now has an innovated spring-loaded outlet check-valve.<h4>Gradient function</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2007, ECB2007PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support</h4>The pumps are supported by ECOMAC and Clarity software.<h3>Main features and benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Heating unit for pump heads available</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg" alt="ECOM: ECP2100 - Preparative Pump - Pressure Compensation Learning algorithm " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECP_2200_Preparative_Pump_5_b84832f1e8.jpg 188w," sizes="100vw" width="700" height="580"><h3>Optional configurations/variants</h3>Pump head heating unit, see the accessories shown below. If you would like this function for your pump, add the heating unit to&nbsp;your cart. (Also can be found in the Accessories product category).PN / Wetted parts of pumping block<ul><li>ACCH000X / Hastelloy</li><li>ACCP000X /<span style="-webkit-text-stroke-width:0px;display:inline !important;float:none;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;"> PEEK</li><li>ACC0000X / Stainless steel</li><li>ACCT000X / Titanium</li></ul><h4>Parameters:</h4><ul><li>Flow rate: 0.1 – 100 ml/min</li><li>Maximum operating pressure: 30 MPa (4351 psi)</li><li>Flow rate setting: 0.001 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 0,5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Communication: RS232, Ethernet (LAN), USB, Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 250VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 12 (26,5 lb)</li><li>Output capillary outer diameter: 1/16”</li><li>Upper pressure limit (MPa): 1– 30 (4351PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Display, keypad: yes&nbsp;</li><li>Pressure Compensation Learning: yes</li></ul></div>

ECOM ECP2300 Preparative Pump

<div style="display:block;" aria-labelledby="tab_item-0">Powerful and durable pumps suitable for preparative applications in low- and high-pressure liquid chromatography across the chemical industry. An extended&nbsp;number of wetted material versions – not only stainless steel but also Hastelloy, titanium, and PEEK.&nbsp;ECP2300 are designed with 2 pump heads connected in parallel with a piston diameter of 3/8”.Many improvements make these pumps more reliable, including a new learning algorithm for pulsation suppression and a leakage sensor. The pumps can operate in two modes, i.e. using constant pressure or using the newly added option of constant flow rate.<h4>Gradient function:&nbsp;</h4>These pumps work as isocratic pumps, and, together with ECOM gradient boxes, they also work as gradient pumps. Gradient boxes ECB2005, and ECB2005PC are equipped with a four-way gradient mixing valve and must be ordered together with the pump. These sophisticated units allow for achieving a precise low-pressure gradient, i.e. gradual changes of solvent composition by mixing up to four liquids at the pump‘s entry. The gradient profile can be defined via a computer or manually using a display and a keypad. Without the use of a gradient box, the pump works as isocratic, with the option of one solvent and the composition of the mobile phase being chosen by the user.Customers can use the pistons‘ back-washing function, after working, for example, with a buffered mobile phase.<h4>Seals:&nbsp;</h4>All pumps are delivered with GFP (PTFE) seals by default. You can order the pumps with UHMW-PE seals, which are considered the optimal recommended choice.<h4>Software support:&nbsp;</h4>The pumps are supported by ECOMAC and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a>.<h3>Features and Benefits</h3><ul><li>All models include a display and keyboard for manual control</li><li>Precise low-pressure gradient mixing (when connected with a gradient module)</li><li>Very good durability and reliability with proper maintenance</li><li>Extended number of wetted material versions – not only stainless steel but also Hastelloy, titanium and PEEK for biocompatible applications</li><li>Piston back-washing function for work with buffered or aggressive mobile phases</li><li>New learning algorithm for pulsation suppression</li><li>Integrated leakage sensor</li><li>Custom pump modifications of request</li></ul>This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).This pump is equipped with the Pressure Compensation Learning algorithm. Thanks to this smart adaptive process, the pump regulates the rotation speed of the camshaft and measures its impact on pressure pulsation to minimize the output pulsation as much as possible. The algorithm, due to its adaptive capacity, is applicable for many combinations of pump working conditions (solvents, columns, etc.).Service videos -Seals replacement and&nbsp;Valve replacement :&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.ecomsro.com/support/service-videos">https://www.ecomsro.com/support/service-videos</a><h4>Parameters:</h4><ul><li>Flow rate: 0.001 – 300 ml/min</li><li>Maximum operating pressure: 15 MPa (2176 psi)</li><li>Flow rate setting: 0.001 ml/min steps</li><li>Repeatability of flow rate adjusting: ± 0.5 %</li><li>Accuracy of flow rate setting: ± 2 %</li><li>Communication: RS232,Ethernet (LAN),USB,Remote digital I/O</li><li>Power supply: 100 240V 50/60Hz 200VA</li><li>Dimensions W x H x D: 280 x 135 x 498 mm (11.02 x 5.12 x 18.23 in)</li><li>Weight: 13 kg (28.7 lb)</li><li>Output capillary outer diameter: 1/8”</li><li>Upper pressure limit (MPa): 1– 15 (2176PSI)</li><li>Precision of pressure measurement: ± 2%</li><li>Supported Gradient Box: ECB2005, ECB2005PC</li><li>Pressure Compensation Learning: yes&nbsp;</li></ul></div>

ECOM LED20M FIX Dual UV Detector with display

The LED20M FIX Dual UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.

<div style="display:block;" aria-labelledby="tab_item-0">The LED20M FIX Dual UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.<h3>Why choose LED20M FIX detectors for your application</h3><ul><li>The LED detectors allow easy and convenient detection in the UV-VIS range. They are manufactured in single or dual channel models with fixed wavelength setup.</li><li>The detectors are widely used in chromatography techniques using gravity glass columns for purification and isolation of organic small molecules and other compounds of interest. Long-lasting and reliable light source is provided by LED.</li><li>The optical flow cell is easily accessible and replaceable, this feature allows flexible choice of detection sensitivity if required. Low cell heating due to low power dissipation of LEDs makes this detector ideal for applications where analytes may be susceptible to degradation due to elevated temperatures. Absorbance information is displayed in detail on the integrated OLED display in numerical and graphical formats.</li><li>The units have convenient USB-C connectivity and power. When the detector is connected to a PC, the recorded data can be stored and processed using the ECOMAC software. ECOMAC basic software license is available with the price of the product without any additional fees. All these features and capabilities allow LED20M FIX to be an affordable, cost-effective, reliable and robust choice of an UV-VIS detector.</li></ul><h4>Wavelengths</h4><ul><li>The dual UV detector version measures at two fixed wavelengths, which are adjusted in production and given by the built-in LEDs.</li><li>By default, diodes with wavelengths of 255 and 280 nm are used. Optionally, the customer can select a wavelength in the range of 255–⁠⁠⁠⁠⁠⁠600 nm in steps of 5 nm.</li></ul><h4>Control</h4><ul><li>The detector can work without any additional SW support, but it is also possible to interconnect it via USB-C cable to a PC with ECOMAC SW, to get a data set for later use or to get reports for unit diagnostics.</li><li>The unit has an innovative PCB board that powers the LEDs, controls the unit, and has many new features, including higher sampling speed.</li></ul><h4>Key features and benefits</h4><ul><li>OLED display</li><li>Numerical and graphical view mode</li><li>Long lifetime of the LED light source</li><li>Low cell heating due to low power losses of the LEDs</li><li>USB C powering and communication port</li><li>ECOMAC Basic software for free</li><li>Autozero function</li><li>Easy flow cell replacement</li><li>Small dimensions</li><li>ECOMAC SW - &nbsp;sophisticated software for data acquisition and for diagnostics</li></ul></div>

ECOM Separtrix PP03 Preparative Pump

Pulse-less triplex piston pumps designed for preparative chromatography applications, equipped with unique compact three-piston head for smooth flow and easy maintenance.

Separtrix PP03 are pulse-less triplex piston pumps designed for preparative chromatography applications - nevertheless, they can be used everywhere, where high pressure, precise flow without pulsations, and high resistance against corrosion are needed. Pumps are equipped with unique compact three-piston head for smooth flow and easy maintenance without a risk of leakage on connecting parts. Back flushed pistons are covered by a hard and chemically resistant polycrystalic carbon layer. Piston cylinders have low dead volume and samples injected through the pump appear low spreading.Pumps with different flow rate ranges differ only in head type, piston diameters, and tubing diameters. Separtrix PP03 are usually supplied with an asynchronous motor and frequency converter but are also available with a step motor which allows the use of the pump in an extremely broad range of flow rates - both for analytical and preparative applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_4_6079b99e0f.jpg" alt="ECOM: Separtrix PP03 Preparative Pump" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_4_6079b99e0f.jpg 245w," sizes="100vw" width="1349" height="503">Special configurations of Separtrix PP03 pumps are used for three phases low pressure gradient – in this case is extremely important that these pumps offer a nearly constant flow rate not only on the output but on the input too. Low-pressure gradient units are delivered with three solenoid valves on the plate which can be connected to the pump side. The power supply for valves is installed inside the pump box. 10 linear steps can be programmed.Separtrix PP03 pumps are delivered in configuration for high-pressure binary gradient as well. Two pumps – master and slave types – are working together. The master pump controls the flow rate and gradient composition (10 linear steps), slave pump is delivered without a control display, keyboard(s), and pressure sensor. All Separtrix PP03 pumps are equipped with keyboard(s) and graphic display. There is a possibility to set flow rate, pressure limit, and flow correction. A second keyboard is included for gradient programming in the case of low-pressure gradient pumps and high-pressure gradient master pumps.Separtrix PP 03 can be used in the mode of remote control. Pumps can cooperate with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity data station (Dataapex)</a>. Flow rate, gradient composition, and pressure limit can be programmed. The control module for PP 03 pumps was successfully tested and agreed upon by Dataapex. PP 03 pumps can be controlled using Ecom software ECOMAC as well.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_2_ce1c6f0f8f.jpg" alt="ECOM: Separtrix PP03 Preparative Pump" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ECOM_ECOM_Separtrix_PP_03_Preparative_Pump_2_ce1c6f0f8f.jpg 245w," sizes="100vw" width="1377" height="832"><h3>Types &amp; Description with technical parameters</h3>SEPARTRIX PP03 A: Piston pulsless pump, three pistons O.D. 10 mm with a diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor vector frequencyconverter, flow rate 25 ml/min. – 800 ml/min., 260 bar (3500 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 B: Piston pulsless pump, three pistons O.D. 14 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 50 ml/min. – 1600 ml/min., 160 bar (2100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 A: Piston pulsless pump, three pistons O.D. 10 mm with a diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor vector frequencyconverter, flow rate 25 ml/min. – 800 ml/min., 260 bar (3500 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 B: Piston pulsless pump, three pistons O.D. 14 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 50 ml/min. – 1600 ml/min., 160 bar (2100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 C: Piston pulsless pump, three pistons O.D. 20 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 100 ml/min. – 3200 ml/min., 80 bar (1100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 AG: PP03 A pump, flow rate 25 ml/min. – 800 ml/min., max. 260 bar (3500 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder + 3 x valve power supply in pump unit), second keyboard for gradient control&nbsp;SEPARTRIX PP03 BG: PP03 B pump, three phases, flow rate 50 ml/min. – 1600 ml/min., max. 150 bar (2100 PSI), low-pressure gradient included (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03 C: Piston pulsless pump, three pistons O.D. 20 mm with diamond layer in one stainless steel head, ceramic ball valves, 620 W AC motor with vector frequency converter, flow rate 100 ml/min. – 3200 ml/min., 80 bar (1100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03 AG: PP03 A pump, flow rate 25 ml/min. – 800 ml/min., max. 260 bar (3500 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder + 3 x valve power supply in pump unit), second keyboard for gradient control&nbsp;SEPARTRIX PP03 BG: PP03 B pump, three phases, flow rate 50 ml/min. – 1600 ml/min., max. 150 bar (2100 PSI), low-pressure gradient included (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03 CG: PP03 C pump, flow rate 100 ml/min. – 3200 ml/min., 80 bar (1000 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03S A: Piston pulsless pump, three pistons O.D 10 mm with diamond layer in one stainless steel head, ceramic ball valves, step motor, flow rate 1 ml/min. – 400 ml/min., 250 bar (3500 PSI), pressure measurement and limitSEPARTRIX PP03S B: Piston pulsless pump, three pistons O.D 14 mm with diamond layer in one stainless steel head, ceramic ball valves, step motor, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03S C: Piston pulsless pump, three pistons O.D 20 mm with diamond layer in one stainless steel head, ceramic ball valves, step motor, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), pressure measurement and limit, membrane keyboard, graphic displaySEPARTRIX PP03S AG: PP03S A pump, flow rate 1 ml/min. – 400 ml/min., 250 bar (3500 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03S BG: PP03S B pump, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03S CG: PP03S C pump, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), three phases low-pressure gradient included, (3 x solenoid valves + connecting armature on own holder+3 x valve power supply in pump unit), second keyboard for gradient controlSEPARTRIX PP03SM A: PP03S A pump, flow rate 0,5 ml/min. – 400 ml/min., 250 bar (3500 PSI), master pump for binary high-pressure gradient, second keyboard for gradient programming&nbsp;SEPARTRIX PP03SM B: PP03S B pump, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), master pump for binary high-pressure gradient, second keyboard for gradient programmingSEPARTRIX PP03SM C: PP03S C pump, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), master pump for binary high-pressure gradient, second keyboard for gradient programmingSEPARTRIX PP03SS A: PP03SS A pump, flow rate 0,5 ml/min. – 400 ml/min., 250 bar (3500 PSI), slave pump for binary gradient, no display, no keyboard&nbsp;SEPARTRIX PP03SS B: PP03SS B pump, flow rate 1 ml/min. – 800 ml/min., 150 bar (2100 PSI), slave pump for binary gradient, no display, no keyboardSEPARTRIX PP03SS C: PP03SS C pump, flow rate 2 ml/min. – 1600 ml/min., 70 bar (800 PSI), slave pump for binary gradient, no display, no keyboard

ECOM LED20M FIX Single UV Detector with display

The LED20M FIX Single UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.

<div style="display:block;" aria-labelledby="tab_item-0">The LED20M FIX Single UV Detector with a display uses Light Emitting Diodes (LED) as the light source. It is used for flash chromatography.<h3>Why choose LED20M FIX detectors for your application</h3><ul><li>The LED detectors allow easy and convenient detection in the UV-VIS range. They are manufactured in single or dual channel models with fixed wavelength setup.</li><li>The detectors are widely used in chromatography techniques using gravity glass columns for purification and isolation of organic small molecules and other compounds of interest. Long-lasting and reliable light source is provided by LED.</li><li>The optical flow cell is easily accessible and replaceable, this feature allows flexible choice of detection sensitivity if required. Low cell heating due to low power dissipation of LEDs makes this detector ideal for applications where analytes may be susceptible to degradation due to elevated temperatures. Absorbance information is displayed in detail on the integrated OLED display in numerical and graphical formats.</li><li>The units have convenient USB-C connectivity and power. When the detector is connected to a PC, the recorded data can be stored and processed using the ECOMAC software. ECOMAC basic software license is available with the price of the product without any additional fees. All these features and capabilities allow LED20M FIX to be an affordable, cost-effective, reliable and robust choice of an UV-VIS detector.</li></ul><h3>Wavelengths</h3><ul><li>The single UV detector version measures at 1 fixed wavelength, which is adjusted in production and given by the built-in LEDs.</li><li>By default, diodes with wavelengths of 255 or 280 nm are used. Optionally, customers can select a wavelength within the range of 255–⁠⁠⁠⁠⁠⁠600 nm in steps of 5 nm.</li></ul><h3>Control</h3><ul><li>The detector can work without any additional SW support, but it is also possible to interconnect it via USB-C cable to a PC with ECOMAC SW, to get a data set for later use or to get reports for unit diagnostics.</li><li>The unit has an innovative PCB board that powers the LEDs, controls the unit, and has many new features, including higher sampling speed.</li></ul><h3>Key features and benefits</h3><ul><li>Practical OLED display</li><li>Numerical and graphical view mode</li><li>Long lifetime of the LED light source</li><li>Low cell heating due to low power losses of the LEDs</li><li>USB -C powering and communication port</li><li>Autozero function</li><li>Easy flow cell replacement</li><li>Small dimensions</li><li>ECOMAC SW - &nbsp;sophisticated software for data acquisition and for diagnostics</li></ul>Note: A preparative cell PFC 200 Basic is supplied with the unit. Other flow cells are available at request.</div>

Related content

Investigating the Effect of Isoelectric Points on the Gas-Phase Stability of Native-like Proteins Analyzed in Positive- versus Negative-Ion Mode by IMS-MS

Regulated Drinking Water Testing: PFAS Detection, Sample Prep & Real Lab Insights

I waited two years for a start-up grant and wrote ten versions of my project idea