🎤Alex BrodyIn this episode of Concentrating on Chromatography, we sit down with Alex Brody, an organic chemist at Aqua America, to discuss the rigorous analytical methods required for regulated drinking water testing.Alex walks us through the multi-step PFAS detection process using EPA Method 537.1, including extraction, nitrogen blowdown concentration, and LC-MS analysis. He explains why sample preparation and quality control are critical for achieving trace-level detection—and why these methods can't be rushed or simplified, even with new technologies available.We also explore taste and odor investigations, disinfection byproducts, and volatile organic compounds, plus the surprising reality of why regulated labs move slowly when adopting new instrumentation. You'll learn about the quality control checkpoints, peer review processes, and the misconceptions surrounding analytical turnaround times in compliance labs.<h5>Topics Covered:</h5><ul><li>Role of organic chemistry in regulated drinking water labs</li><li>EPA compliance requirements and regulatory bodies (EPA, PA-DEP)</li><li>PFAS detection using Method 537.1 and LC-MS</li><li>The critical importance of nitrogen blowdown sample concentration</li><li>Taste and odor analysis: MIB, Geosmin, and unknown compound identification</li><li>Why regulated methods evolve slowly (validation timelines, approval processes)</li><li>Quality control procedures: calibration checks, matrix spikes, surrogate standards, internal standards</li><li>Automated sample preparation and lab efficiency</li><li>Future PFAS regulations and Method 533 expansion</li></ul><h5>Ideal For:</h5><ul><li>Analytical chemists and environmental lab professionals</li><li>Water utilities and compliance officers</li><li>Chromatography practitioners interested in regulated workflows</li><li>Anyone curious about how drinking water safety is ensured</li></ul><h2>Video Transcription</h2><h3>Ensuring Safe Drinking Water Through Advanced Analytical Chemistry</h3><h4>Insights from a Regulated Water Testing Laboratory</h4>Analytical laboratories responsible for regulated drinking water testing operate under strict compliance frameworks to ensure the delivery of safe and reliable water supplies. In this interview, Alex, an organic chemist at Aqua America, outlines the scope of analytical work conducted in a modern water quality laboratory. The team primarily focuses on the determination of taste- and odor-causing compounds, disinfection byproducts, volatile organic compounds (VOCs), and per- and polyfluoroalkyl substances (PFAS). These analyses rely heavily on GC-MS and LC-MS instrumentation, supported by rigorous maintenance programs to ensure continuous operational performance and data reliability.<h4>Regulatory Compliance and Quality Control in Drinking Water Analysis</h4>Operating as a regulated drinking water laboratory requires adherence to stringent requirements established by agencies such as the U.S. Environmental Protection Agency (EPA) and state-level regulatory bodies. Compliance is driven by extensive quality control protocols integrated into every analytical batch. Typical workflows include calibration verification, matrix spike recovery assessments, internal standard monitoring, and surrogate performance checks. Notably, analytical batches are often limited in sample number to accommodate a substantial proportion of quality control samples, reflecting the critical importance of data defensibility in regulatory reporting.One common misconception is the perceived speed at which results can be delivered. In reality, comprehensive sample preparation, including extraction procedures and validation steps, can extend turnaround times to a full working day or longer. Additionally, validated regulatory workflows impose limitations on the rapid adoption of new analytical technologies, as method modifications must undergo formal evaluation and approval before implementation.<h4>Analytical Strategies for Taste and Odor Investigations</h4>Taste and odor events represent a significant operational challenge for water utilities. Standard targeted methods are routinely applied for compounds such as 2-methylisoborneol (MIB) and geosmin, which are commonly associated with earthy or musty sensory characteristics. When targeted analysis does not reveal the source, laboratories employ broad-scan GC-MS screening combined with spectral library interpretation to identify potential causative compounds. In some cases, sensory evaluation panels complement instrumental analysis, providing preliminary insight into potential treatment-related or environmental influences affecting water quality.<h4>PFAS Monitoring: Methodological Complexity and Trace-Level Sensitivity</h4>PFAS determination represents a rapidly evolving priority in drinking water monitoring. The laboratory currently applies EPA Method 537.1, with efforts underway to expand capabilities through certification for Method 533, thereby broadening analyte coverage. Analytical workflows typically involve solid-phase extraction followed by solvent evaporation using nitrogen-assisted blow-down techniques to achieve the trace-level sensitivity required for regulatory compliance. This concentration step is essential because PFAS compounds remain non-volatile under controlled evaporation conditions, enabling enhanced detection performance upon reconstitution and LC-MS analysis.<h4>Future Directions in Drinking Water Analytics</h4>Looking ahead, the primary technological driver in regulated water analysis is the demand for increasingly lower detection limits and expanded compound coverage. While regulatory frameworks can slow methodological innovation, laboratories continue to pursue efficiency improvements, such as automated sample preparation systems and optimized analytical workflows. These advances support the growing need to process high sample volumes while maintaining the stringent data quality standards required for public health protection.Ultimately, the work performed in regulated drinking water laboratories represents a critical intersection of analytical chemistry, environmental monitoring, and public safety. Continuous methodological refinement, coupled with advances in mass spectrometry and sample preparation technologies, will play a central role in addressing emerging contaminants and ensuring long-term water quality assurance.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Regulated Drinking Water Testing: PFAS Detection, Sample Prep & Real Lab Insights

This study investigates how ionization polarity influences the gas-phase stability of native-like proteins analyzed by ion mobility spectrometry–mass spectrometry. While proteins generally retain folded structures in both positive and negative electrospray ionization modes, significant differences in unfolding behavior were observed.Proteins exhibited greater gas-phase stability when analyzed in the ionization polarity corresponding to their solution-phase charge, requiring higher energy for unfolding. These findings emphasize the importance of considering protein isoelectric points and ionization polarity in native IMS-MS experiments to ensure accurate structural characterization.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295">Investigating the Effect of Isoelectric Points on the Gas-Phase Stability of Native-like Proteins Analyzed in Positive- versus Negative-Ion Mode by IMS-MS</a></h4>Alexis N. Edwards, Madeline G. Bannon, Michael S. Cordes, and Elyssia S. Gallagher*Anal. Chem. 2026, 98, 6, 4518–4527<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.analchem.5c04295">https://doi.org/10.1021/acs.analchem.5c04295</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.<div>In native mass spectrometry (nMS), the native-like structures of proteins are maintained in the gas phase by preserving the secondary, tertiary, and quaternary structures. <a href="javascript:void(0);">(1−7)</a> The preservation of native-like protein structures is primarily accomplished via the use of electrospray ionization (ESI), a soft ionization method that forms a distribution of multiply charged protein ions. These multiply charged ions can then be investigated to characterize stoichiometry, oligomeric composition, and ligand binding. <a href="javascript:void(0);">(8−10)</a>During ESI, a potential is applied to the sample-containing solution. In an aqueous spray solvent, H3O+ and OH– are produced in positive-ion mode (+ESI) and negative-ion mode (−ESI), respectively. <a href="javascript:void(0);">(11)</a> The buildup of like-charges within the solution results in the formation of a Taylor Cone at the capillary tip, which ejects charged droplets that travel toward the inlet of the mass spectrometer. Droplets undergo evaporation and fission until complete analyte desolvation, leading to the release of folded, gas-phase protein ions. As the droplets shrink, H3O+ or OH– can become concentrated, causing the pH of the droplet to decrease or increase, respectively. Shifts in pH can denature proteins. Therefore, to maintain native-like protein structures, nMS is conducted with solutions containing volatile salts, usually ammonium acetate, that provide ionic strength and minimize pH changes compared to spraying from pure water. <a href="javascript:void(0);">(11,12)</a> Droplet pH in ESI is influenced by flow rate, solvent composition, and applied voltage, <a href="javascript:void(0);">(13)</a> but often approaches values near the pKa of acetate (4.76) or ammonium (9.25) in +ESI or −ESI, <a href="javascript:void(0);">(12,14)</a> respectively. During ESI, changes in overall protein charge take place via proton transfer at chargeable amino acid residues, resulting in the formation of protonated or deprotonated protein ions with the charged sites being dependent on the relative proton affinities of ammonium, acetate, and chargeable amino acids as proteins near complete desolvation. <a href="javascript:void(0);">(15−19)</a>Ion mobility spectrometry (IMS) measures an ion’s mobility through an inert gas under the influence of an electric field. Thus, mobility is directly influenced by the ion’s size, shape, and charge. <a href="javascript:void(0);">(20)</a> A protein’s mobility can be used to estimate its structure by calculating an average momentum-transfer collisional cross section (CCS). In addition to structural characterization, IMS-MS can investigate the gas-phase stability of protein complexes when paired with collision-induced unfolding (CIU). Specifically, in CIU experiments, protein ions are accelerated into a cell filled with inert gas molecules and undergo multiple collisions with higher frequency and energy relative to IMS. This converts kinetic energy to internal, vibrational energy, disrupting noncovalent interactions and inducing unfolding of the protein ions. <a href="javascript:void(0);">(21)</a> Unfolding alters the protein structure, which can then be quantified by monitoring the protein’s mobility during subsequent IMS-MS measurement. <a href="javascript:void(0);">(22)</a> Prior work with mutant proteins has shown that proteins with similar intramolecular interactions have similar CIU fingerprints, while proteins with different intramolecular interactions have different CIU fingerprints. <a href="javascript:void(0);">(23)</a> Therefore, gas-phase protein stability is correlated with intramolecular interactions.Past native IMS-MS work has primarily been conducted in +ESI given that -ESI has the reputation of being unreliable for native-like protein analysis due to the occurrence of corona discharge, <a href="javascript:void(0);">(24)</a> and charge-state distributions that may not be representative of folded proteins. <a href="javascript:void(0);">(25)</a> In recent years, −ESI has been increasingly used in CIU and IMS-MS, with recent work demonstrating that −ESI-MS is capable of discerning denatured and native-like protein structures. <a href="javascript:void(0);">(26,27)</a> Prior work showed that charge manipulation of folded proteins, such as charge reduction, to achieve similar absolute charge states in both ion modes, results in CCS values of protein cations that are comparable to their anion counterparts. <a href="javascript:void(0);">(26,28)</a> This suggests that gas-phase structures are weakly dependent on protein charge and ionization polarity. <a href="javascript:void(0);">(23,26,28)</a> Yet, Hong et al. showed that, in the absence of charge reduction, the gas-phase stability of two proteins (avidin and β-lactoglobulin) is sensitive to ionization polarity, highlighting that gas-phase stability is not consistent between +ESI and −ESI. <a href="javascript:void(0);">(29)</a><h4>Materials and Methods</h4><h5>Native IMS-MS Analysis</h5>Glass capillaries were pulled using a P-1000 micropipette puller (Sutter Instrument Company, Novato, CA). Samples and calibrants were sprayed from pulled capillaries (inner diameter ∼1 μm) and analyzed with a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/152">Synapt G2-S High-Definition MS </a>(Waters Corporation, Milford, MA). The trap and transfer cells contained Ar, the helium cell contained He, and the IMS cell contained N2. IMS wave velocity was 350 m/s, while IMS wave height was either 7 or 9 V, depending on the protein size (see <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Table S2</a>). Proteins with larger mass required a wave height of 9 V to ensure that all the ions migrated out of the IMS cell prior to the next ion injection. Mass spectra were collected and signal averaged over 30 s. Additional instrument details can be found in the Supporting Information (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Table S2</a>).To limit systematic error due to repositioning of the ESI tip between runs, <a href="javascript:void(0);">(48)</a> the XYZ-stage position was optimized and kept consistent for all replicates. Approximately 5 μL of sample was loaded into a glass capillary, which was then mounted on the XYZ-stage. Electric potential was delivered to the sample by a platinum wire inserted into the capillary and placed in contact with the solution. The voltage applied to the tip holder ranged from 0.60 to 1.2 kV for native samples, and 0.80 to 1.40 kV for denatured samples. Nine replicates in each ion mode were collected for all proteins with measurements spread across 8 days over 3 weeks. These replicates were conducted in pairs by collecting data in both ion modes consecutively. To accomplish this, a CIU experiment was collected in +ESI before the instrument polarity was changed to −ESI. A second CIU experiment was performed, beginning with −ESI to counteract any changes in protein stability due to pH changes during ESI. <a href="javascript:void(0);">(14)</a> This allowed for use of the same capillary, at the same position in relation to the instrument inlet, for each +ESI/–ESI replicate pair. A new tip was used for each +ESI/–ESI replicate pair. CIU experiments were accomplished by increasing collision voltage (CV) to the trap cell in 5 V steps, from 0 V to a maximum of 120 V.<h4>Results and Discussion</h4>Ten proteins were chosen for analysis in +ESI and −ESI in this work (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#tbl1">Table 1</a>). These proteins can be broken into three groups depending on the net-charge in solution at pH 7: net-negative (with pI &lt; 6.5), net-neutral (with 6.5 ≤ pI ≤ 7.5), and net-positive (with pI &gt; 7.5). Five of the ten proteins have a net-negative charge at pH 7, though four of these proteins (con A, β-lactoglobulin, ovalbumin, and α-lactalbumin) are routinely analyzed via +ESI-IMS-MS. <a href="javascript:void(0);">(18,28)</a> Additionally, each protein had charge-state distributions in +ESI and −ESI that resulted in at least one overlapping charge state (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#fig1">Figure 1</a>), allowing for direct comparison of TWCCSN2→He and CIU50 in both ion modes.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2026_98_6_4518_4527_Figure_1_Representative_mass_spectra_in_ESI_top_and_ESI_bottom_cb81976247.jpg" alt="Anal. Chem. 2026, 98, 6, 4518–4527: Figure 1. Representative mass spectra in +ESI (top) and −ESI (bottom) for (A) ovalbumin, (B) GNA, (C) β-lactoglobulin, (D) α-lactalbumin, (E) con A, (F) streptavidin, (G) ubiquitin, (H) WGA, (I) cytochrome c, and (J) lysozyme. Proteins that have blue or red charge states indicate additional oligomeric states that were detected. Outlines in red boxes are overlapping charge states that are compared herein. *Indicates contaminant peaks." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2026_98_6_4518_4527_Figure_1_Representative_mass_spectra_in_ESI_top_and_ESI_bottom_cb81976247.jpg 245w," sizes="100vw" width="1600" height="865"><h5>Proteins with Disulfide Bridges are Stable in Both Polarities</h5>For both lysozyme and α-lactalbumin, the average TWCCSN2→He were within experimental uncertainty in +ESI and −ESI for both the initial, folded structures and the final, extended structures (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#tbl2">Table 2</a>). However, the 6+ and 6- ions of lysozyme and α-lactalbumin showed a high resistance to unfolding with &gt;90% of the ions retaining their folded TWCCSN2→He values even at high CV (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#fig2">Figure 2</a>). Replicate data showed similar trends (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Figure S2</a>). While CCS values provide a description of gas-phase structures, CIU experiments provide information on the stability of the gas-phase protein structures. Furthermore, the number and pattern of disulfide bridges present in antibodies has been characterized by CIU. <a href="javascript:void(0);">(63)</a> Lysozyme and α-lactalbumin both contain the glycosyl hydrolases family 22 (GH22) domain, which has four disulfide bridges that span ∼94% and ∼93% of the amino acid sequence, respectively (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Figure S3</a>). Thus, we hypothesize that rather than effects from protein pI and ionization polarity, the unfolding behavior of these proteins is controlled to a greater extent by the presence of stabilizing disulfide bridges that prevent routine unfolding during collisional activation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Anal_Chem_2026_98_6_4518_4527_Figure_2_Representative_mobiligrams_showing_arrival_times_for_lysozyme_A_C_and_a_lactalbumin_B_D_0f3d23c3c1.jpg" alt="Anal. Chem. 2026, 98, 6, 4518–4527 - Figure 2. Representative mobiligrams showing arrival times for lysozyme (A, C) and α-lactalbumin (B, D) at low and high CV (blue solid and orange dashed lines, respectively) for +ESI (A, B) and −ESI (C, D). For lysozyme, different values were chosen for the high CV (e.g., 540 eV in +ESI and 480 eV in −ESI) because these were the last analyzed CV where the signal-to-noise ratio (S/N) for the 6 ± ions was greater than 3." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Anal_Chem_2026_98_6_4518_4527_Figure_2_Representative_mobiligrams_showing_arrival_times_for_lysozyme_A_C_and_a_lactalbumin_B_D_0f3d23c3c1.jpg 212w," sizes="100vw" width="1600" height="1178">To characterize the stabilizing effect of disulfides, MD simulations were conducted that heat denatured 6+ ions of lysozyme and α-lactalbumin with disulfide bridges either present or removed with the resulting cysteine residues being protonated prior to heating (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Figure S4</a>). Structural changes associated with heat denaturation were characterized by taking the root-mean-square deviation (RMSD) of the heated structures relative to initial, crystal structures. Over ten trials, lysozyme ions possessed an average RMSD of (1.6 ± 0.2) nm when disulfides were present which increased to (3.5 ± 0.5) nm when disulfides were removed. Similarly, the RMSD of α-lactalbumin ions increased from (1.8 ± 0.1) nm when disulfides were present to (3.7 ± 0.3) nm when disulfides were removed, suggesting that the disulfides serve as a barrier to unfolding. This is visually represented in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Figure S4C,D</a> where simulated structures of heated lysozyme and α-lactalbumin ions with intact disulfides adopt more condensed configurations relative to those with disulfides removed. In essence, the disulfides “tie” disparate regions of protein together, which serves as a barrier to more thorough unfolding even at a maximum simulated temperature of 900 K, explaining the similarity of TWCCSN2→He values in +ESI versus −ESI as well as the limited percentage of ions that undergo a change in TWCCSN2→He upon additional collisional activation.WGA has 16 disulfide bridges per monomeric subunit (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#tbl1">Table 1</a>). On average, these disulfides encompass seven amino acids each, with ∼78% the total amino acids in WGA being encompassed by at least one disulfide bridge (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.analchem.5c04295/suppl_file/ac5c04295_si_001.pdf">Figure S3</a>). Compared to lysozyme and α-lactalbumin, the presence of disulfide bridges across small numbers of amino acids enables WGA to unfold with ΔCCS of (370 ± 60) Å2 and (390 ± 120) Å2 in +ESI and −ESI, respectively (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.analchem.5c04295#tbl2">Table 2</a>). However, in comparison to the other lectins, GNA and con A, we hypothesize that these disulfide bridges in WGA limit the extent of unfolding, resulting in the extended, TWCCSN2→He values being within experimental uncertainty in both ion modes.To summarize, lysozyme and α-lactalbumin exhibit similar gas-phase behavior in both +ESI and −ESI, with most ions retaining folded structures even under high CV. We hypothesize this resistance to unfolding is largely attributed to their four disulfide bridges, which span over 90% of their sequences and act as stabilizing constraints. MD simulations confirm that removing these disulfides significantly increases structural deviation during heat denaturation, indicating their critical role in maintaining compact configurations. Consequently, the presence of disulfide bridges explains the minimal changes in TWCCSN2→He and the proteins’ high stability under activation.<h4>Conclusions</h4>IMS-MS allows for detailed analysis of the structure and stability of native-like proteins in the gas phase. The structure of a protein is dictated by its amino acid sequence and the resulting noncovalent interactions that form the secondary, tertiary, and quaternary structures. Chargeable amino acid residues that are solvent exposed can carry charges leading to the protein having a net-positive, net-negative, or net-neutral charge in solution at physiological pH. Yet, most native IMS-MS analysis is conducted in +ESI, leading to proteins that are net-negative in solution experiencing large swings in their net-charge during the ESI process.This work highlights that gas-phase stability of native-like proteins varies with ionization polarity. While CCS values for folded structures are generally comparable between +ESI and −ESI, unfolding energetics differ─net-negative proteins are more stable in −ESI, whereas net-positive proteins are more stable in +ESI. We hypothesize that these differences are due to changes in the intramolecular interactions within cationic and anionic proteins. Therefore, proteins analyzed in the mode opposite to their solution-phase charge may have different gas-phase structures compared to biological systems. For example, proteins that are net-negatively charged in physiological environments (pI &lt; 6.5) are more likely to represent their biological counterparts as gas-phase anions when analyzed in −ESI compared to +ESI, enabling nMS experiments to more closely characterize the structures present in biological systems.</div>

Investigating the Effect of Isoelectric Points on the Gas-Phase Stability of Native-like Proteins Analyzed in Positive- versus Negative-Ion Mode by IMS-MS

In the week of March 30, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 500 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Bruker: MALDI Imaging Becomes IR-resistible: Going Multimodal for Advanced Biomedical Insights</h5><ul><li>Mo, 30.3.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5971">Registration</a></li></ul>Explore advanced multimodal imaging with MALDI, QCL-based IR, and fluorescence microscopy to achieve ROI-focused molecular insights in complex tissues.<h5>2. Shimadzu Corporation: Environmental PFAS Testing: Introducing Next-Generation Mass Spectrometry Solutions</h5><ul><li>Tu, 31.3.2026 09:30 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6026">Registration</a></li></ul>Explore advanced PFAS analysis strategies combining LC-MS/MS and MALDI-TOF screening to improve sensitivity, efficiency, and regulatory readiness in environmental testing.<h5>3. Tosoh Bioscience: Tosoh Tuesday - Scaling Up from Process Development to GMP Production with Pre-packed Columns</h5><ul><li>Tu, 31.3.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6089">Registration</a></li></ul>This webinar will introduce SkillPak™ MAX pre-packed columns and present performance data using TOYOPEARL® Super A, a novel Protein A resin.<h5>4. Phenomenex: Recipe for Separation: Crafting Your Perfect HPLC Method</h5><ul><li>We, 1.4.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6091">Registration</a></li></ul>Discover how to properly use and maintain your column to maximize its performance and lifespan.

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Chiral ion exchanger GEN-QD is highly efficient in the enantiomer resolution of acidic compounds.This product is now available in high quality and at a reasonable price at our company!To create our chiral sorbent, we use 3µm fully porous spherical silica particles, which provide greater efficiency than the original product.Additionally, our Galo-ION-QD-200 is recommended for biological applications due to its larger pore size, which improves the separation efficiency for large molecules.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QD_HPLC_kolony_ION_QD_selector_10dd9c7ee5.jpg" alt="Galochrom: Galochrom Chiral ION-QD selector."><h5>Premium Quality</h5>In every manufactured product, we guarantee the highest standards of quality.<h5>Cost-effective</h5>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.<h5>High-performance</h5>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.<h3>Ordering information</h3><blockquote>Stationary phase Chiral ION-QD is provided in these column types: analytical and preparative</blockquote><h4>Analytical Columns</h4><ul><li>Dimensions [mm]: 150 x 3</li><li>Porosity: [Å] 200</li><li>Cat. No.: ION02-A150</li><li>Price [€]: 1 300</li></ul><blockquote><h4>Get up to a 20% discount on your first purchase with us.</h4></blockquote><h3>Reach new heights</h3>Spend more time on testing and less time on troubleshooting<blockquote><h4>How can our unique columns help you succeed?</h4>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:<ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4>What makes Chiral <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1241">ION-QN</a> and ION-QD unique?</h4>Designed for stabilityThe manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376">During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"><h3>Achieve your results</h3>Increase your productivity with uniform, stable, and reproducible results<h4>High-Quality Silica</h4>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a result, our columns:<ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"><h4>Elution Order</h4>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers. Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545">The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:<ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

Galochrom Chiral ION-QN HPLC columns

These HPLC columns are based on high-quality spherical silica from the world's leading silica manufacturer from Japan. 

Our company is proud to offer the Galo ION-QN chiral ion exchanger, which is capable of efficiently separating enantiomers of acidic compounds.This product is now available in high quality and at a reasonable price at our company!To create our chiral sorbent, we use 3µm fully porous spherical silica particles, which provide greater efficiency than the original product.Additionally, our Galo-ION-QN-200 is recommended for biological applications due to its larger pore size, which improves the separation efficiency for large molecules.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Galochrom_Chiral_ION_QN_HPLC_kolony_ION_QN_selector_f3789cb2c1.jpg" alt="Galochrom: Galochrom Chiral ION-QN selector."><h5>Premium Quality</h5>In every manufactured product, we guarantee the highest standards of quality.<h5>Cost-effective</h5>Our unique products optimize your budget &amp; enhance efficiency in your laboratory operations.<h5>High-performance</h5>Besides their outstanding performance, our HPLC columns also expand your separation possibilities.<h3>Ordering information</h3><blockquote>Stationary phase Chiral ION-QN is provided in these column types: analytical and preparative</blockquote><h4>Analytical Columns</h4><ul><li>Dimensions [mm]: 150 x 3</li><li>Porosity: [Å] 200</li><li>Cat. No.: ION01-A150</li><li>Price [€]: 1 300</li></ul><blockquote><h4>Get up to a 20% discount on your first purchase with us.</h4></blockquote><h3>Reach new heights</h3>Spend more time on testing and less time on troubleshooting<blockquote><h4>How can our unique columns help you succeed?</h4>Chiral ION-QN and Chiral ION-QD empower researchers with their daily tasks, such as:<ul><li>Seamless method development</li><li>Enantioseparation</li><li>Quality control</li><li>Monitoring of asymmetric reactions</li><li>Intermolecular interaction studies</li><li>Pharmacokinetic studies</li><li>Preparative separation</li><li>Production scale-up</li><li>Efficient purification</li></ul></blockquote><h4>What makes Chiral ION-QN and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1240">ION-QD</a> unique?</h4>Designed for stabilityThe manufacturing of Chiral ION-QN and Chiral ION-QD was designed to produce highly reproducible and reliable HPLC columns. Our high standards in synthesis of organic selectors and robust packing technology ensures that each customer receives identical high-performance product.The chromatographic results are reproducible from run-to-run, column-to-column and batch-to-batch.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_1_9dea7516f4.jpg 245w," sizes="100vw" width="655" height="376">During our manufacturing, we implement strict quality control measures over all aspects from silica chemistry up to column packing. As a result, almost no variation is observed among manufactured batches of chiral stationary phases.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Designed for stability." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Designed_for_stability_2_50c42d67e6.jpg 245w," sizes="100vw" width="1176" height="442"><h3>Achieve your results</h3>Increase your productivity with uniform, stable, and reproducible results<h4>High-Quality Silica</h4>Our columns contain fully-porous spherical particles from world’s leading manufacturer based in Japan. These ultra-pure silica particles have narrow size distribution to ensure reproducible, robust and reliable results.In comparison to other manufacturers, Galochrom’s custom packing technology produces very stable and uniform bed with intact silica particles. As a result, our columns:<ul><li>Enhance productivity and resolution</li><li>Provide accurate and consistent performance</li><li>Generate significantly higher plate counts</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - High-Quality Silica." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_High_Quality_Silica_522d14ecf7.jpg 245w," sizes="100vw" width="617" height="386"><h4>Elution Order</h4>Chiral ION-QN and Chiral ION-QD represent a pair of columns that enable the reversal of elution order of enantiomers. Now you can position your desired enantiomer first and easily achieve 100% purity at higher pace.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg" alt="Galochrom: Chiral ION-QN &amp; Chiral ION-QD HPLC columns - Elution Order." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Galochrom_Chiral_ION_QN_and_Chiral_ION_QD_HPLC_columns_Elution_Order_85c5b02411.jpg 245w," sizes="100vw" width="953" height="545">The elution order was investigated with other QN/QD derivatives using enantiomeric pairs of Leucine derivatives. The conclusions for the first eluent are following:<ul><li>Chiral ION-QN ⇒ L-Enantiomer</li><li>Chiral ION-QD ⇒ D-Enantiomer</li></ul>

SIELC Alltesta Gradient Automated HPLC Analyzer

SIELC’s Alltesta™ Analyzer is an affordable HPLC analytical system with for measuring small and trace concentrations of various chemicals.

SIELC’s Alltesta™ Analyzer is an affordable HPLC analytical system that provides both scientific and educational environments with a convenient, simple solution for measuring small and trace concentrations of various chemicals in products such as milk, baby formula, pet food, pills, household formulations, and more.No difficult sample preparation is necessary, and no prior experience in chromatography or analytical chemistry is required for the operator. Measurements typically take less than 5 minutes, and the Alltesta™ comes with a set of supplies sufficient for 200 analyses.<ul><li>Small foot-print, light-weight, can be used on a small table</li><li>No difficult sample preparation</li><li>Takes only a few minutes to start operating</li><li>No chemical experience is required for instrument user</li><li>User friendly, self explanatory cloud-based software</li><li>Produces easy-to-understand results within 5-10 minutes</li></ul><h4>Alltesta Gradient Automated HPLC Analyzer includes:</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1249">Alltesta™ Power Tower</a> (provides power and cloud connectivity)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1244">Alltesta Mini Autosampler</a> (includes <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1245">Alltesta High-Pressure Switching Valve</a>)</li><li>Two <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1247">Alltesta™ Ultra High Pressure Mini Syringe Pumps</a> for gradient flow</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1248">Alltesta UV-Vis Detector</a></li></ul><h4>Examples of Alltesta™-based HPLC methods</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Aspirin_in_Alka_Seltzer_Extra_Strength_Effervescent_Aspirin_on_Primesep_100_Column_d33816ae83.pdf">HPLC Method for Analysis of Aspirin in Alka-Seltzer Extra Strength Effervescent Aspirin</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Phenylephrine_Hydrochloride_1_in_Equate_Nasal_Spray_on_Primesep_100_Column_02f2983c25.pdf">HPLC Method for Analysis of Phenylephrine Hydrochloride 1% in Equate – Nasal Spray</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Rockstar_Zero_Carbs_Energy_Drink_with_Caffeine_and_Taurine_on_Primesep_100_Column_c87a85a0b4.pdf">HPLC Method for Analysis of Rockstar Zero Carbs Energy Drink with Caffeine and Taurine</a></li></ul>See this cloud-based example of the 2-Naphthalenesulfonic acid, Allura red, Fast Green FCF, 1,5-Naphthalenedisulfonic acid, Tartrazine separation method <a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/11972/injection/21307/?t=a992fefcdd3647b19f6bed81e182d3d8">here</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_example_of_the_2_Naphthalenesulfonic_acid_Allura_red_Fast_Green_FCF_1_5_Naphthalenedisulfonic_acid_Tartrazine_separation_b96e648d63.jpg" alt="SIELC: SIELC Alltesta™ Gradient Automated Analyzer - example of the 2-Naphthalenesulfonic acid, Allura red, Fast Green FCF, 1,5-Naphthalenedisulfonic acid, Tartrazine separation.">

SIELC Alltesta Cromite HPLC Analyzer

Compact and cloud-connected, the Cromite™ HPLC System delivers precise UV/Vis analysis with innovative single-ended column technology—ideal for research, QC, and education labs.

The Cromite™ HPLC System from SIELC Technologies is a compact, high-performance liquid chromatography platform engineered for research, teaching, and routine quality control. It combines professional analytical performance with intuitive operation in a lightweight, space-saving design.Designed for academic institutions, research laboratories, and QC environments in pharmaceuticals, food science, and environmental analysis, Cromite™ delivers reliable separations without the complexity of conventional full-scale systems. Its automated single-vial injection, isocratic syringe pump, and UV/Vis detection enable accurate analysis of compounds such as caffeine, vitamins, food dyes, environmental contaminants, and active pharmaceutical ingredients.Cloud-enabled control software allows users to create methods, run analyses, review chromatograms, and share data remotely from virtually any device. This connectivity makes Cromite™ well suited for distributed teams, teaching environments, and flexible laboratory setups.A key innovation is SIELC’s proprietary Single-Ended HPLC Column technology (up to 150 mm length). This design eliminates tubing and fittings, minimizes dead volume, and enables tool-free column replacement, reducing setup time and the risk of leaks. Each system is supplied with a single-ended column of choice and all essential accessories for immediate operation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_Alltesta_Cromite_HPLC_analyzator_285f593640.jpg" alt="SIELC: Alltesta Cromite HPLC analyzer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_Alltesta_Cromite_HPLC_analyzator_285f593640.jpg 234w," sizes="100vw" width="1536" height="1024"><h4>Key Features</h4><ul><li>Compact and Lightweight Design: Weighing only 1.45 kg and measuring 115 × 190 × 115 mm, Cromite™ fits easily into space-limited labs and classrooms.</li><li>Automated Injection: Single-vial automated injection simplifies operation and improves reproducibility.</li><li>Isocratic Syringe Pump: Flow rates from 0.1 to 4.0 mL/min with pressures up to 4000 psi ensure effective separation of complex mixtures.</li><li>UV/Vis Detection<ul><li>Standard wavelengths: 275, 460, 520, 630 nm</li><li>Custom configuration: any four wavelengths starting from 235 nm</li><li>Low noise (&lt;0.05 mAU) and 20 Hz detection rate support reliable quantification.</li></ul></li><li>Cloud-Based Software: Wi-Fi, Ethernet, and mobile connectivity enable remote method development, data analysis, and collaboration.</li><li>Single-Ended Column Technology<ul><li>Zero dead volume design</li><li>No tubing or fittings</li><li>Tool-free replacement</li><li>Supports 4.6, 3.2, and 2.1 mm ID columns up to 150 mm length</li></ul></li></ul><blockquote>We offer a special 15% discount for schools.</blockquote><h4>Examples of Alltesta™-based methods</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12579/injection/22619?t=3f67e3c308cc479085c836cc682e8f1a">HPLC Method of Analysis of Ascorbic Acid</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12533/injection/22589/?t=43cad31c0e924440a18874658565c3db">HPLC Method of Analysis of Theobromine, Theophylline, Caffeine and C7</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12411/injection/22415/?t=0c93f4eb855c43aebcfd4c3b1fd55ce9">HPLC Method of Analysys of Aspirine</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Phenylephrine_Hydrochloride_1_in_Equate_Nasal_Spray_on_Primesep_100_Column_02f2983c25.pdf">HPLC Method for Analysis of Phenylephrine Hydrochloride 1% in Equate – Nasal Spray</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Rockstar_Zero_Carbs_Energy_Drink_with_Caffeine_and_Taurine_on_Primesep_100_Column_c87a85a0b4.pdf">HPLC Method for Analysis of Rockstar Zero Carbs Energy Drink with Caffeine and Taurine</a></li></ul><h4>Specifications</h4><ul><li>Injection: Automatic, 1 vial</li><li>Pump: Syringe, isocratic</li><li>Flow Rate: 0.1–4.0 mL/min</li><li>Maximum Pressure: 4000 psi</li><li>Noise Level: &lt;0.05 mAU</li><li>Detection Rate: 20 Hz</li><li>Wavelengths (standard): 275, 460, 520, 630 nm</li><li>Wavelengths (custom): Any four starting from 235 nm</li><li>Supported Columns: Single-Ended HPLC Columns (4.6, 3.2, 2.1 mm ID)</li><li>Column Length: ≤150 mm</li><li>Connectivity: Wi-Fi, Ethernet, Mobile network</li><li>Power Supply: 100–240 V, 50–60 Hz</li><li>Operating Temperature: 0–40 °C</li><li>Dimensions (W × H × L): 115 × 190 × 115 mm</li><li>Weight: 1.45 kg</li></ul><h4>Typical Applications</h4><ul><li>Pharmaceutical Analysis – API identification, formulation testing, and stability studies</li><li>Environmental Testing – Detection of contaminants in water and soil</li><li>Food &amp; Beverage – Analysis of vitamins, additives, and preservatives</li><li>Academic Laboratories – Hands-on chromatographic training and research</li><li>Materials &amp; Chemical Research – Evaluation of additives, catalysts, and specialty chemicals</li></ul><blockquote>For pricing information, please contact: <a>alltesta@sielc.com</a></blockquote>

SIELC Alltesta Mini HPLC Autosampler

The Alltesta™ Mini Autosampler is an affordable and dependable solution for automated sample handling in various analytical and liquid handling applications, including HPLC.

The Alltesta™ Mini Autosampler is an affordable and dependable solution for automated sample handling in various analytical and liquid handling applications, including HPLC. With its proprietary alpha/beta mechanical design, this compact autosampler offers direct and comprehensive control of its features, making integration quick and easy. Users can tailor the mini autosampler to their needs with customizable valve configurations, tray designs, and syringe volumes and pressures. Additionally, a built-in shaking feature enables sample mixing before injection and/or simple sample extraction within the vial.
This compact autosampler comes designed with original equipment manufacturer (OEM) customization in mind. Custom shape, color, and communication interfaces can be adapted to integrate with any system. Comprehensive software integration support is provided. Other options include metal-free sampling and reagent addition and mixing capabilities.
<ul>
<li>FRACTION COLLECTION CAPABILITY: A simple software modification can quickly convert this small autosampler into a fraction collector.
</li>
<li>REACTOR PROBE SAMPLER CAPABILITY: A simple software modification can quickly convert this small autosampler into a reactor probe sampler.
</li>
</ul>
This miniature autosampler can also be used as a component of our <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> and <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a>.
<blockquote>
<h4>The price of the Alltesta™ Mini Autosampler varies depending on the specific configuration requested.</h4>
</blockquote>
<h4>Specifications</h4>
<ul>
<li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li>
<li>Weight: 3 kg / 6.6 lb</li>
<li>Power: 24 V</li>
<li>Capacity: 48 vials or 96-well plate</li>
<li>Pressure: 5000 psi</li>
<li>Needle Wash: Inside and outside</li>
<li>Syringe Capacity: 120µL high-pressure / 4000µL low-pressure stainless / 4000µL low-pressure ceramic</li>
<li>Valve options: 6×2, 7×6 and 10×9 (more details here).</li>
<li>Volume Accuracy: 0.1µL</li>
<li>Communication: RS232 Virtual COM port via USB</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_Alltesta_Mini_Autosampler_A_very_simple_schematic_operation_of_the_Alltesta_Autosampler_b79b0ad187.jpg" alt="SIELC: Alltesta™ Mini Autosampler - A very simple schematic operation of the Alltesta™ Autosampler."/>
<h4>Software</h4>
The Alltesta™ Autosampler can be controlled:
<ul>
<li>directly via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Autosampler_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 5Mb; <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Autosampler_Control_Windows_7.zip">Windows 7, 32/64bit</a>, 126Mb).</li>
<li>remotely through our cloud software (as a part of Alltesta™ Analyzer or Alltesta™ Mini Analyzer),</li>
</ul>
Serial communication drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.

SIELC Alltesta Mini High-Pressure Switching HPLC Valve

The Alltesta™ Mini High-Pressure Switching Valve offers versatility and convenience for laboratory fluid management applications.

The Alltesta™ Mini High-Pressure Switching Valve offers versatility and convenience for laboratory fluid management applications. It features flexible flow switching at varying flow rates and can be used independently or as part of the <a href="https://lcms.labrulez.com/products/124">Alltesta™ Analyzer</a> or <a href="https://lcms.labrulez.com/products/1244">Alltesta™ Autosampler</a>.
With customizable options, including up to 10 available ports on the stator, the valve can be tailored to specific needs. It is built to handle pressures up to 5000 psi (350 bar) and boasts a fast 60 degree switch time of 200 milliseconds.
<h4>Specifications</h4>
<ul>
<li>Power requirements: USB type B</li>
<li>Dimensions (WHL): 63 x 46 x 128 mm / 2.48 x 1.81 x 5.04 in</li>
<li>Weight: 0.45 kg / 0.95 lb</li>
<li>Switching speed for 60 degree: 200 ms</li>
<li>Max pressure: 350 bar (5000 psi)</li>
<li>Ports style: 10-32 thread with conical 1/16″ tube compression type seal</li>
<li>Materials in contact with liquid: coated SS316, Vespel® or PEEK or PTFE</li>
</ul>
<h4>Available Configurations</h4>
<ul>
<li>6x2 Valve</li>
<li>7x6 Valve</li>
<li>10x9 Valve</li>
</ul>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Mini_High_Pressure_Switching_Valve_Available_Configurations_72e3b13ea2.jpg" alt="SIELC: SIELC Alltesta™ Mini High-Pressure Switching Valve - Available Configurations."/>
<h4>Software</h4>
The Alltesta™ High-Pressure Switching Valve can be controlled:
<ul>
<li>directly via serial commands (option with greatest flexibility, Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our software, allowing to use the whole functionality of the unit without the need for automation (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Valve_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 6 Mb, <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Valve_Control_Windows_7.zip">Windows 7, 32/64 bit</a>, 127 Mb),</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Analyzer</a>)</li>
<li>operator may also simply press the button on the front of the device to switch it to the next position</li>
</ul>
Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.
<h4>Available Custom Options</h4>
<ul>
<li>A High-flow version of the valve (preparative) is available</li>
<li>A metal-free version of the valve is also available.</li>
</ul>
<h4>Valve Rotor Position Indication</h4>
LED color indicates the valve’s rotor position. By pressing the LED, the rotor can be advanced by one position. Depending on the valve configuration, the second press will either advance the rotor forward or backward. Position 1 – LED Green Position 2 – LED Navy Position 3 – LED Purple Position 4 – LED Blue Position 5 – LED Red Position 6 – LED White
<h4>Applications</h4>
<ul>
<li>Column Switching</li>
<li>Solvent Selection</li>
<li>Sample Injection</li>
<li>Sample Cleaning</li>
</ul>

SIELC Alltesta™ Mini LHS (Liquid Handling Station)

The Alltesta™ Mini Liquid Handling Station serves as an affordable, reliable solution for automated sample handling.

The Alltesta™ Mini Liquid Handling Station serves as an affordable, reliable solution for automated sample handling. With its compact design, proprietary alpha/beta mechanical structure, and adjustable operation speeds, it offers comprehensive control for quick and seamless integration with other equipment. The station comes equipped with a built-in sample shaking feature for in-vial sample mixing and extraction, and a needle washing station capable of accommodating up to four solvents.
Designed with Original Equipment Manufacturer (OEM) customization in mind, the Alltesta™ Mini Liquid Handling Station can be tailored to fit any system’s requirements. Custom needle lengths and trays enable the device to accommodate various types of vials. An array of integrated valves and a measuring syringe can be added to the device. Additional customization options, such as metal-free flow paths and customer-specified enclosure colors, are also available.
Comprehensive software integration support is provided to ensure smooth operation and functionality.
<h4>Specifications</h4>
<ul>
<li>Dimensions (WHL): 160 x 145 x 195 mm / 6.3 x 5.71 x 7.68 in</li>
<li>Weight: 2.25 kg / 5 lb</li>
<li>Power: 24 V</li>
<li>Capacity: 48-vial tray or 96-well plate</li>
<li>Needle Wash: Inside and Outside</li>
<li>Communication: RS232 Virtual COM port via USB</li>
</ul>
<h4>Software</h4>
The Alltesta™ LHS can be fully controlled via serial commands. Alltesta™ protocol available upon request, please contact us at support@sielc.com. Serial communication drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.

SIELC Alltesta Mini Syringe Pump

Alltesta™ Ultra High Pressure Mini Syringe Pump is designed to produce a steady, high pressure, pulsation-free flow, so there is no need for bulky dampeners, mixers, and precision check valve arrangements.

Alltesta™ Ultra High Pressure Mini Syringe Pump is designed to produce a steady, high pressure, pulsation-free flow, so there is no need for bulky dampeners, mixers, and precision check valve arrangements. It can refill prior to each run in less than a minute, and can produce flow rates from as low 1 μL/min to as fast as 4000 μL/min with pressure up to 4000 psi (285 bar).
The Mini Syringe Pump allows for several communication options via its USB/Serial port. Just like the rest of our Alltesta™ line of devices, our serial protocol allows to control and automate every function of the device. We also provide free Windows-based software allowing full control of the device.
<h4>Specifications</h4>
<ul>
<li>Syringe volume: 5.8 mL</li>
<li>Power Requirements 24 VDC</li>
<li>Current Requirements Up to 1.5 A</li>
<li>Dimensions (WHL) 63x167x128 mm / 2.48×6.57×5.04 in</li>
<li>Weight 1.65 kg / 3.7 lb</li>
<li>Flow Rate Range 0.001 to 4 mL/min</li>
<li>Speed Accuracy 1% of full-scale</li>
<li>Linearity Full Range 0.001 to 4.0 mL/min</li>
<li>Pressure 4000 psi (285 bar) continuous</li>
<li>Pressure accuracy ±1 psi</li>
<li>Operating Temperature 0 to 40° C, non-condensing</li>
<li>Inlet/Outlet Thread 10-32 UNF compression type</li>
<li>Materials in contact with liquid SS316, PTFE, PEEK</li>
<li>Communication USB virtual serial port</li>
</ul>
<h4>Software</h4>
The Mini Syringe Pump can be controlled:
<ul>
<li>directly through your software via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> and <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a>.),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Pump_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 5Mb; <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Pump_Control_Windows_7.zip">Windows 7, 32/64bit</a>, 126Mb)</li>
</ul>
Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.
<h4>Available Custom Options</h4>
Metal-free version of the pump includes no metal contact pump head and can be operated up to 4000 psi.

SIELC Alltesta Mini UV-Vis Detector

The Alltesta™ UV-Vis Multi-wavelength Mini Detector is a compact and versatile device that can continuously monitor the optical properties of solutions flowing through it.

The Alltesta™ UV-Vis Multi-wavelength Mini Detector is a compact and versatile device that can continuously monitor the optical properties of solutions flowing through it. The Mini Detector is ideal for use in HPLC, Flow Injection Analysis (FIA), and preparative and flash chromatography.
The device comes standard with four different wavelength channels, including 470 nm (Blue), 520 nm (Green), 630 nm (Red), and a choice between 255 nm or 275 nm for the UV channel. The Mini Detector features a simple setup process with a USB interface that allows it to communicate with the <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> or <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a> or integrate with other instrumentation.
Powered solely by its USB port, this mini detector is capable of monitoring multiple analytes simultaneously. It has a low optical cell volume of &lt; 3 µL, which results in minimal dispersion even at low flow rates, and can collect data at rates of up to 20 sps for all four channels, making it suitable for demanding applications. The Mini Detector boasts low noise and linearity over more than 4 orders of magnitude.
In addition to its compact size and versatility, the Mini Detector also has zero warm-up time and exceptional durability. The detector cell volume is available in two sizes: 3 μL and 10 μL.
<h4>Specifications</h4>
<ul>
<li>Dimensions (WHL): 63 x 46 x 116 mm / 2.48 x 1.81 x 4.57 in</li>
<li>Weight: 0.25 kg / 0.55 lb</li>
<li>Sample Cell Volume: &lt; 3µL</li>
<li>Cell Optical Path: 7.0mm</li>
<li>Liquid Ports: 10-32 UNF</li>
<li>Wavelength Range: 250nm &amp; up</li>
<li>Simultaneous Wavelengths: Up to 4</li>
<li>Data Rate: 20 sps</li>
<li>Noise Level: 0.03 mAU</li>
<li>Power: USB Type B</li>
</ul>
<h4>Software</h4>
The Multi-wavelength Detector can be controlled:
<ul>
<li>directly via serial commands (Alltesta™ protocol available upon request, please contact us at support@sielc.com),</li>
<li>directly via our demo software, allowing to learn the whole functionality of the unit (<a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Detector_Control_Windows_10.zip">Windows 10, 32/64bit</a>, 5Mb, <a href="https://sielc.com/wp-content/uploads/2022/12/Alltesta_Detector_Control_Windows_7.zip">Windows 7, 32/64bit</a>, 127Mb)</li>
<li>remotely through our cloud software (as a part of <a href="https://lcms.labrulez.com/products/1242">Alltesta™ Gradient Automated Analyzer</a> and <a href="https://lcms.labrulez.com/products/1243">Alltesta™ Cromite Analyzer</a>).</li>
</ul>
Serial Communication Drivers are available <a href="https://ftdichip.com/drivers/vcp-drivers/">here</a>.

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