🎤Dr. Leigh Terry (University of Alabama)In this episode of Concentrating on Chromatography, we sit down with Dr. Leigh Terry from the University of Alabama to explore one of the biggest challenges in environmental analysis today: understanding—and accurately measuring—PFAS in water systems.While most regulatory methods focus on a small number of compounds like PFOA and PFOS, there may be thousands of PFAS compounds in the environment—and much of that burden goes undetected.<h4>In this episode, we cover:</h4><ul><li>Why PFAS are called “forever chemicals” (and why that label is evolving)</li><li>The limitations of targeted LC-MS/MS methods</li><li>What the fluorine mass balance problem reveals about PFAS destruction</li><li>How combustion ion chromatography (CIC) helps measure total organofluorine</li><li>Why “99.99% removal” doesn’t always mean what you think</li><li>Where PFAS actually end up in wastewater treatment systems</li><li>The most effective technologies for PFAS removal today</li><li>Why drinking water may only account for ~20% of total PFAS exposure</li></ul><h4>Key Insight:</h4>Even when analyzing dozens of PFAS compounds, researchers may only be capturing a small fraction of total organic fluorine—raising major questions about how we monitor and regulate these contaminants.<h3>Video Transcription</h3>In this episode of Concentrating on Chromatography, Dr. Leigh Terry discusses PFAS contamination in drinking water, the analytical challenges associated with measuring these compounds, current treatment technologies, and future directions for monitoring and regulation. The conversation highlights how analytical chemistry, environmental engineering, and public health intersect in addressing one of today's most discussed classes of emerging contaminants.<h4>Understanding PFAS and Their Significance</h4><h5>The University of Alabama Water Quality Research Center</h5>Dr. Leigh Terry is part of the University of Alabama's Center for Water Quality Research. The center operates an analytical laboratory focused on drinking water quality and the detection of contaminants at extremely low concentrations.The laboratory specializes in:<ul><li>Drinking water analysis</li><li>Organic contaminants</li><li>Emerging contaminants</li><li>PFAS monitoring</li><li>Water quality research</li></ul>Their work supports both research initiatives and practical solutions for water utilities and environmental agencies.<h4>Why Focus on PFAS?</h4>Although PFAS compounds have existed since the 1940s, public awareness has increased dramatically only in recent years.According to Dr. Terry, several factors contributed to the rapid growth of PFAS research:<ul><li>Increased media attention</li><li>New regulatory initiatives</li><li>Growing evidence of human exposure</li><li>Expanding research opportunities</li><li>Greater understanding of potential health risks</li></ul>PFAS are not new contaminants. Rather, society is only beginning to understand their prevalence in the environment and their potential impacts on human health.<h4>The Development of Standardized PFAS Methods</h4>One major advancement in PFAS analysis has been the development of standardized EPA methods.These methods allow:<ul><li>Consistent testing procedures</li><li>Comparable results between laboratories</li><li>Regulatory monitoring</li><li>Reliable data interpretation</li></ul>Dr. Terry notes that many of these standardized approaches began appearing around 2018–2019, providing a common framework for analytical laboratories and researchers.<h4>How Large Is the PFAS Universe?</h4>Many people are familiar with PFOA and PFOS, two of the most heavily studied PFAS compounds. However, these represent only a small fraction of the broader PFAS family.PFOA and PFOS are often referred to as:<ul><li>Legacy PFAS compounds</li><li>Original PFAS contaminants</li><li>Early commercial PFAS chemicals</li></ul>The challenge is that even minor modifications to molecular structure create entirely new PFAS compounds.Researchers estimate:<ul><li>Thousands of PFAS compounds exist</li><li>Some estimates exceed 10,000 compounds</li><li>Exact numbers depend on how PFAS are defined</li></ul>Scientific debate continues regarding classification and definition, making precise counting difficult.<h4>Why PFAS Are Called "Forever Chemicals"</h4>Dr. Terry notes that the phrase "forever chemicals" has become widely used, although she suggests the terminology could benefit from rebranding.PFAS persistence results from two key chemical properties.<h5>1. Amphiphilic Structure</h5>PFAS molecules contain:<ul><li>A water-loving (hydrophilic) head group</li><li>A water-repelling (hydrophobic) fluorinated tail</li></ul>This unusual combination allows PFAS to partition between:<ul><li>Water</li><li>Solids</li><li>Air-water interfaces</li><li>Foam</li></ul>Unlike many organic compounds, PFAS can interact effectively with multiple environmental phases.<h5>2. Extremely Strong Carbon–Fluorine Bonds</h5>The carbon–fluorine bond is among the strongest bonds in organic chemistry.As Dr. Terry explains:<ul><li>Large amounts of energy are required to break these bonds</li><li>Natural degradation processes are often ineffective</li><li>Destruction can require highly aggressive treatment conditions</li></ul>These properties make PFAS extremely persistent in both environmental systems and biological organisms.<h4>Toxicity, Regulations, and Environmental Behavior</h4><h5>Understanding PFAS Toxicity</h5>One of the biggest challenges in PFAS regulation is the incomplete understanding of toxicity.Dr. Terry explains that EPA regulations generally consider three factors:<ul><li>Presence in drinking water sources</li><li>Toxicity to humans</li><li>Availability of practical treatment options</li></ul>While some PFAS compounds are known to be harmful, many others remain insufficiently studied. Furthermore, PFAS often occur as mixtures rather than individual compounds.This creates important scientific questions:<ul><li>What is the toxicity of individual PFAS?</li><li>What is the toxicity of PFAS mixtures?</li><li>How should cumulative exposure be evaluated?</li></ul>These uncertainties continue to drive active research.<h4>What Happens to PFAS in Wastewater Treatment Plants?</h4>PFAS do not behave like many conventional contaminants.Rather than remaining fully dissolved in water, they often accumulate at interfaces such as:<ul><li>Water-solid interfaces</li><li>Water-air interfaces</li><li>Foam layers</li><li>Biological solids</li></ul>As a result:<ul><li>Some PFAS accumulate in biosolids</li><li>Some partition into foam</li><li>Some remain in treated effluent</li></ul>Their distribution depends on wastewater composition and treatment processes.<h4>Regulatory Monitoring and Targeted LC-MS Analysis</h4>Current EPA regulations focus on targeted analysis of specific PFAS compounds.Examples include:<ul><li>PFOA</li><li>PFOS</li></ul>Drinking water regulations currently establish maximum contaminant levels (MCLs) for these compounds at extremely low concentrations.Advantages of targeted LC-MS analysis include:<ul><li>Clearly defined analytes</li><li>Established regulatory limits</li><li>High analytical sensitivity</li><li>Reliable quantitation</li></ul>However, limitations remain:<ul><li>Only a small subset of PFAS is measured</li><li>Many PFAS compounds remain undetected</li><li>Total PFAS burden may be underestimated</li></ul>As Dr. Terry notes, targeted analysis provides only part of the overall picture.<h4>The Fluorine Mass Balance Problem</h4>One of the most important concepts discussed in the interview is fluorine mass balance.When PFAS degrade, fluorinated fragments may remain in the environment.This creates a critical question:Are treatment technologies truly destroying PFAS, or merely transforming them into shorter-chain PFAS compounds?To answer this, researchers perform fluorine mass balance calculations using:<ul><li>Targeted PFAS analysis</li><li>Fluoride measurements</li><li>Total organic fluorine measurements</li></ul>A successful destruction process should ultimately convert PFAS into fluoride ions rather than simply producing smaller PFAS molecules.<h4>Advanced Analysis, Treatment Technologies, and Future Directions</h4><h5>Combining Targeted and Bulk Fluorine Measurements</h5>Dr. Terry's group complements targeted LC-MS analysis with measurements of total organic fluorine.Approaches include:<ul><li>Absorbable Organic Fluorine (AOF)</li><li>Extractable Organic Fluorine (EOF)</li><li>Combustion Ion Chromatography (CIC)</li></ul>In these methods:<ul><li>Organic fluorine is captured</li><li>Samples are combusted</li><li>Released fluoride is quantified</li></ul>This provides insight into total fluorinated content, although contributions may originate from sources beyond PFAS, including pharmaceuticals and consumer products.<h4>Surprising Findings from Combined Analysis</h4>One important finding from wastewater studies was that targeted PFAS measurements accounted for only a small fraction of total organic fluorine.Dr. Terry reports that:<ul><li>Approximately 4% of measured AOF could be explained by targeted PFAS compounds</li><li>The majority of organic fluorine remained unidentified</li></ul>This suggests that environmental fluorine chemistry is considerably more complex than currently represented by routine monitoring programs.<h4>Preventing PFAS Contamination in the Laboratory</h4>PFAS analysis requires exceptionally strict quality control.Dr. Terry's laboratory routinely:<ul><li>Tests every new batch of SPE cartridges</li><li>Tests carbon tubes before use</li><li>Uses DI water blanks</li><li>Verifies consumables labeled as PFAS-free</li><li>Collects field blanks</li><li>Monitors analytical blanks throughout workflows</li></ul>These practices help prevent contamination from laboratory materials and consumables, a common challenge in ultra-trace PFAS analysis.<h4>Current PFAS Treatment Technologies</h4>Three technologies currently dominate PFAS removal in drinking water treatment:<ul><li>Granular Activated Carbon (GAC)</li><li>Ion Exchange Resins</li><li>Reverse Osmosis (RO)</li></ul>Selection depends on:<ul><li>PFAS concentration</li><li>Water quality</li><li>Local operating conditions</li><li>Utility infrastructure</li></ul>Although many emerging technologies are being investigated, these remain the most widely implemented solutions.<h4>Membrane Filtration and Pretreatment</h4>Dr. Terry collaborates with colleagues studying membrane-based PFAS treatment.Her own work focuses on pretreatment through biological filtration.The strategy involves:<ul><li>Removing natural organic matter</li><li>Reducing membrane fouling</li><li>Improving membrane performance</li><li>Extending operational efficiency</li></ul>This integrated approach demonstrates how multiple treatment technologies can work together to improve PFAS removal.<h4>Regional Monitoring Projects</h4>The University of Alabama team participates in EPA-supported monitoring efforts along the Gulf Coast.Their observations indicate:<ul><li>PFAS rarely occur alone</li><li>Multiple PFAS compounds frequently co-occur</li><li>Contamination often originates from identifiable point sources</li></ul>Examples include:<ul><li>Industrial facilities</li><li>Manufacturing operations</li><li>Military installations</li><li>Carpet manufacturing facilities</li></ul>Source identification remains a critical component of environmental monitoring programs.<h4>Future Directions for PFAS Monitoring</h4>Looking ahead, Dr. Terry sees several opportunities for improvement.Desired advances include:<ul><li>In situ monitoring technologies</li><li>Portable detection systems</li><li>Reduced analytical complexity</li><li>Lower instrument costs</li><li>Less expensive consumables</li><li>Faster analysis workflows</li></ul>Current PFAS testing requires substantial expertise, specialized instrumentation, and rigorous quality control, making widespread implementation challenging.<h4>Dr. Terry's Perspective on Long-Term Solutions</h4>When asked what major PFAS challenge she would most like to solve, Dr. Terry offered a broader perspective.Rather than focusing solely on remediation, she emphasized prevention.Her view is that society should:<ul><li>Better evaluate chemical toxicity before commercialization</li><li>Assess risks before large-scale deployment</li><li>Reduce introduction of harmful compounds into the environment</li><li>Support utilities tasked with contaminant removal</li></ul>She notes that water utilities often bear the responsibility of removing contaminants they did not create, highlighting the need for broader societal and regulatory support.<h4>Final Thoughts</h4>Dr. Terry encourages consumers to:<ul><li>Learn where their drinking water originates</li><li>Read annual water quality reports</li><li>Stay informed about local water quality</li><li>Consider appropriate home filtration where needed</li></ul>At the same time, she emphasizes confidence in municipal water systems and the importance of continued investment in water quality research, monitoring, and treatment technologies.<h4>Conclusion</h4>PFAS remain one of the most complex challenges in environmental chemistry and water treatment. Through advanced analytical methods such as LC-MS/MS, combustion ion chromatography, fluorine mass balance approaches, and large-scale monitoring programs, researchers like Dr. Leigh Terry are helping improve our understanding of these persistent contaminants.The interview highlights that solving the PFAS challenge will require a combination of better analytical tools, improved treatment technologies, stronger regulatory frameworks, and a greater emphasis on prevention before contaminants enter the environment.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

PFAS in Water: Why We’re Missing 96% of the Problem

This study evaluates an immobilized enzyme reactor (IMER) containing peptide N-glycosidase from Rudaea cellulosilytica (PNGase Rc) for rapid online deglycosylation of intact glycoproteins. Human glycoproteins and therapeutic antibodies were used as model substrates to assess reactor performance.The PNGase Rc IMER efficiently removed complex sialylated and core-fucosylated glycans, simplifying protein characterization and glycosylation-site analysis. The approach offers a fast and effective solution for bioanalytical workflows, biopharmaceutical quality control, and stability testing of therapeutic proteins.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577">Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor</a></h4>Katarína Molnárová, Petr Novák, Jana Nováková, and Petr Pompach*ACS Omega 2026, 11, 17, 25724–25729<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acsomega.6c00577">https://doi.org/10.1021/acsomega.6c00577</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.<div>Protein glycosylation, in which monosaccharide units are attached to a protein backbone through enzymatic pathways, is involved in many important biochemical processes and is considered one of the most important post-translational modifications. <a href="javascript:void(0);">(1)</a> It affects not only the stability and secretion of proteins, cell–cell interactions, and receptor–ligand recognition mechanisms but also the biological activity, plasma half-life, immunogenicity, pharmacodynamics, and pharmacokinetics of biotherapeutics. <a href="javascript:void(0);">(2−5)</a> Therapeutical monoclonal antibodies (mAbs) are among the best known pharmaceuticals that are based on glycoproteins. <a href="javascript:void(0);">(6)</a> Over 90% of the mAbs are derived from immunoglobulin G (IgG), in which N-glycosylation occurs at Asn297 of the Fc region and is responsible for modulating immune responses and interactions with other proteins. <a href="javascript:void(0);">(7,8)</a> In cancer treatment, mAbs are used to increase the response of the immune system against the tumor cell, thus inhibiting the cancerous cell growth and helping with the destruction and elimination. <a href="javascript:void(0);">(9)</a> Among the most commonly used mAbs are trastuzumab, bevacizumab, cetuximab, or margetuximab, which are used as an additional treatment for breast, colorectal, prostate, or lung cancer. <a href="javascript:void(0);">(10)</a>Mass spectrometry (MS)-based methods have become a method of choice in the analysis of mAbs that can provide information on sequence, post-translational modifications, and glycosylation profiles. <a href="javascript:void(0);">(11)</a> The enzymatic removal of the attached glycans from the protein backbone can reduce the complexity of the MS analysis, which can lead to a better understanding of the protein structure and modifications. In glycoproteomics, peptide-N-glycosidase F (PNGase F) is most used for glycan removal. It cleaves the linkage between the Asn residue and the N-acetylglucosamine (GlcNAc). <a href="javascript:void(0);">(12)</a> Jmeian et al. developed a monolithic PNGase F enzyme reactor fabricated in fused silica using a glycidyl methacrylate-co-ethylene dimethacrylate polymer and integrated it into an LC–MS workflow. The authors demonstrated the applicability of this system for the characterization of released neutral and acidic N-glycans by LC–MS analysis. <a href="javascript:void(0);">(13)</a> In another study, oriented immobilization of PNGase F onto a methacrylate-based monolithic support via glutathione-S-transferase–glutathione affinity coupling was employed to release N-glycans from denatured ribonuclease B and fetuin, as well as from native IgG. The released glycans and the corresponding deglycosylated proteins were subsequently analyzed by MALDI-MS. <a href="javascript:void(0);">(14)</a>Gramlich et al. showed the potential of PNGase Rc for N-glycan hydrolysis from both tryptic peptides and native glycoproteins at a low pH. The favorable properties of PNGase Rc, including activity at acidic pH and compatibility with reducing agents such as tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and denaturants like urea, make this enzyme well suited for online intact protein deglycosylation workflows. <a href="javascript:void(0);">(15,16)</a>In this report, we demonstrate the potential of the PNGase Rc IMER for rapid online deglycosylation of reduced monomeric glycoproteins, including human haptoglobin (Hp), human transferrin, anti-hHD6, and the therapeutic monoclonal antibodies trastuzumab and bevacizumab. The column retained its activity over more than 150 consecutive measurements, highlighting its robustness, suitability for repeated use in bioanalytical workflows, and applicability to pharmaceutical research and development.<h4>Experimental Section</h4><h5>Instrumentation and Experimental Conditions</h5>Automated sample handling and online deglycosylation were carried out using the HDX workstation (AffiPro, Czech Republic). The scheme of the system is shown on <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig1">Figure 1</a>. Samples of 50 mM ammonium bicarbonate buffer (pH 8.2) and 40 mM TCEP were placed in a sample tray at 4 °C. Ten microliters of the protein sample at a concentration of 0.03 mg/mL (0.3 μg per injection) were added to 40 μL of 50 mM ammonium bicarbonate and then transferred to 50 μL of 40 mM TCEP, pH 2.3. The disulfide bond reduction was carried at 40 °C for 5 min. The automated sample handling system injected 100 μL of the reaction mixture into the sample loop and switched the injection valve. Online deglycosylation was performed at room temperature using a PNGase Rc IMER (2.1 × 20 mm, AffiPro, Czech Republic) at a flow rate of 50 μL/min, followed by protein desalting on an OPTI-TRAP (0.5 × 2 mm) trap column (Optimize Technologies Inc., Oregon City, Oregon, USA) heated to 55 °C for 10 min with 0.2% formic acid delivered by an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1260+Infinity+III+isocratic+pump">Agilent 1260 Infinity III isocratic pump</a> (Agilent Technologies, Santa Clara, California, USA). By switching the trap valve, the protein was eluted by an acetonitrile gradient [(min)/% B] 0/10-9/10-16/70-16.5/90-17/90-18/10-23/10] delivered by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+II+LC">Agilent 1290 Infinity II HPLC</a> system (Agilent Technologies, Santa Clara, California, USA) at a flow rate of 20 μL/min. The mobile phase A consisted of 5% acetonitrile with 1% propan-2-ol and 0.1% formic acid, and the mobile phase B consisted of 95% acetonitrile with 1% propan-2-ol and 0.1% formic acid. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=8&amp;search=maXis+Q-TOF">maXis Q-TOF mass spectrometer</a> (Bruker Daltonics, Bremen, Germany) was operated in the survey MS mode in a mass range of m/z 50–3000. The acquired data were analyzed and processed by DataAnalysis 4.4 (Bruker Daltonics) and deconvoluted by UniDec <a href="javascript:void(0);">(17)</a> with the following parameters: data processing range, m/z 500–3000; charge range, 1–80; allowed mass range, 1,000–80,000; sampling rate, 10 Da; peak detection range, 500 Da; and peak detection threshold, 0.1.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_1_Schematic_of_the_automated_sample_handling_system_for_online_protein_deglycosylation_and_desalting_d4ee48b627.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 1. Schematic of the automated sample handling system for online protein deglycosylation and desalting. Protein samples, along with 50 mM ammonium bicarbonate and 40 mM TCEP stock solutions were placed in a sample tray chilled to 4 °C, while a second tray heated to 40 °C was used for disulfide bond reduction with TCEP buffer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_1_Schematic_of_the_automated_sample_handling_system_for_online_protein_deglycosylation_and_desalting_d4ee48b627.jpg 245w," sizes="100vw" width="1287" height="386"><h4>Results and Discussion</h4>The performance of the PNGase Rc IMER for the removal of complex N-glycans from intact proteins was tested using heavily glycosylated human Hp with four glycosylation sites (Asn184, Asn207, Asn211, and Asn241) <a href="javascript:void(0);">(18)</a> located at the β-chain and occupied by complex N-glycans terminated with sialic acid. Haptoglobin phenotype 2-1, used in this study, forms multimers consisting of β-chain and disulfide linked α1 and α2 chains. <a href="javascript:void(0);">(19)</a> The detection of glycosylated Hp β-chain requires reduction of the disulfide bonds by incubating the protein with a reducing reagent. TCEP is a common reducing reagent used for fast reduction of disulfide bonds at various pH levels. Forty degrees Celsius, the maximum temperature of the PAL tray, and 5 min of incubation were sufficient for complete reduction of Hp disulfide bonds. Individual signals corresponding to the glycosylated β-chain were observed in the m/z range of 35,800–37,400 (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig2">Figure 2</a>a). The limited resolution of the mass spectrometer did not allow assigning the different glycoforms of the haptoglobin β-chain, which is occupied by biantennary, triantennary, or tetraantennary glycans with various amounts of sialic acid and fucose. <a href="javascript:void(0);">(20,21)</a> The nonglycosylated α1 and α2 chains were observed at m/z 9190 and 15,940. When the PNGase Rc IMER was integrated into the flow system, a signal at m/z 27,270 was detected, demonstrating complete deglycosylation of the haptoglobin β-chain (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig2">Figure 2</a>b).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_2_A_Mass_spectrum_of_human_Hp_without_the_PN_Gase_Rc_IMER_implemented_in_the_system_0ecca84ed4.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 2. (A) Mass spectrum of human Hp without the PNGase Rc IMER implemented in the system. The base peak signals of the spectrum correspond to both α1 and α2 chains. The signal of the β chain is spread across its different glycoforms. (B) Mass spectrum of human Hp with the PNGase Rc IMER implemented in the system. The signal of the deglycosylated β chain is higher compared to its glycosylated form and observed only at m/z 27,270. This indicates complete removal of glycans from the Hp β chain." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_2_A_Mass_spectrum_of_human_Hp_without_the_PN_Gase_Rc_IMER_implemented_in_the_system_0ecca84ed4.jpg 245w," sizes="100vw" width="2015" height="913">In a previous study by Krenkova et al., the PNGase F IMER was used for deglycosylation of IgG, RNase B, and bovine fetuin with follow-up mass spectrometry detection of both released glycans and intact proteins using MALDI-TOF MS. <a href="javascript:void(0);">(14)</a> The authors demonstrated an effective method for rapid protein deglycosylation, which, however, requires additional sample handling prior to mass spectrometry measurement. The other study by Jmeian et al., which focused on released N-glycans, implemented a PNGase F IMER with online LC–MS detection. <a href="javascript:void(0);">(13)</a> The authors show the effectiveness of the method by identifying many different glycans released from a single protein or from a complex plasma mixture. Our approach is not directly intended for released N-glycan analysis; instead, it demonstrates the ability to measure intact proteins after rapid online deglycosylation using PNGase Rc at low pH. Samples prepared immediately prior to mass spectrometry analysis using the automated sample-handling platform can reduce variability associated with autosampler residence time, as some proteins may undergo degradation or precipitation during storage in the autosampler.Although all glycoproteins in this study were effectively deglycosylated, the method’s efficiency depends on the characteristics of the individual glycoprotein. Therefore, optimizing method parameters─including the isocratic flow rate, glycoprotein incubation time with TCEP, and/or the addition of denaturing reagent─may improve the overall efficiency of the deglycosylation process.The PNGase Rc IMER stability over a series of injections was tested by using the trastuzumab antibody. The measurements were carried out with the enzyme column kept at the laboratory temperature. As shown in <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsomega.6c00577#fig5">Figure 5</a>, the results indicate that the column performance remained stable over the course of the 150 measurements, and no glycosylated form of the trastuzumab antibody heavy chain was detected. The activity of PNGase Rc in the presence of 5% acetonitrile and 5% propan-2-ol was evaluated using a bottom-up approach with human 2–1 haptoglobin, which was online-digested by a coimmobilized nepenthesin-2/pepsin IMER. In the absence of an organic solvent, the peptide IEKVVLHPNYSQVD remained fully deglycosylated throughout 30 injections. In contrast, in the presence of organic solvent, a marked loss of enzymatic activity was observed after only a few injections (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acsomega.6c00577/suppl_file/ao6c00577_si_001.pdf">Figure 2</a>).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ACS_Omega_2026_11_17_25724_25729_Figure_5_Monitoring_the_stability_and_performance_of_PN_Gase_Rc_IMER_4a9b5a9c53.jpg" alt="ACS Omega 2026, 11, 17, 25724–25729: Figure 5. Monitoring the stability and performance of PNGase Rc IMER. Trastuzumab heavy chain was completely deglycosylated after 150 injections." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ACS_Omega_2026_11_17_25724_25729_Figure_5_Monitoring_the_stability_and_performance_of_PN_Gase_Rc_IMER_4a9b5a9c53.jpg 170w," sizes="100vw" width="996" height="918"><h4>Conclusions</h4>Here, we demonstrate the potential of the PNGase Rc IMER for the online removal of N-glycans from intact monomeric glycoproteins reduced with TCEP. The column showed high sensitivity toward Hp, which has multiple glycosylation sites with various glycan structures, confirming its suitability for the profiling of complex glycoproteins. Bevacizumab and trastuzumab exhibited similar glycosylation profiles, with core-fucosylated biantennary glycans being the most abundant. Online deglycosylation with the PNGase Rc column resulted in a complete removal of the glycans from the heavy chains of both antibodies, proving that the column can be used for the rapid online deglycosylation of intact therapeutic mAbs. Moreover, the column remained stable over 150 measurements without any loss of activity, even when it was maintained at laboratory temperature. The significantly reduced reaction time, together with full automation and the absence of manual handling steps, substantially improves analytical throughput compared to conventional off-line deglycosylation workflows. These findings highlight the potential of the PNGase Rc IMER in the development of glycoprotein-based biopharmaceuticals, where detailed structural characterization of the protein part is critical for quality control, monitoring batch-to-batch variability, and stability testing.</div>

Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor

In the week of June 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 500 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Thermo Fisher Scientific: Insights from an IC specialist: To be suppressed or not suppressed?</h5><ul><li>Tu, 30.6.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6310">Registration</a></li></ul>Join Thermo Fisher’s Ask the Experts session to learn how suppressor technology impacts ion chromatography performance, sensitivity, reliability, maintenance, and troubleshooting.<h5>2. SCIEX: Advancing Proteomics with ZenoTOF: From Single-Cell Analysis to Plasma Biomarker Discovery</h5><ul><li>Tu, 30.6.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6311">Registration</a></li></ul>Discover how ZenoTOF high-resolution mass spectrometry is accelerating single-cell proteomics and plasma biomarker discovery through enhanced sensitivity, throughput, and automation.<h5>3. Agilent Technologies: Measuring Diverse Samples with UV/VIS Spectrometers and Integrating Spheres</h5><ul><li>Tu, 30.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6052">Registration</a></li></ul>This webinar will discuss diffuse transmission and reflection UV/VIS measurements, including how to perform measurements with Agilent’s Cary 60 UV/VIS Spectrometer.<h5>4. Waters Corporation: Informatics series #33 Tips, Tricks and Best Practices</h5><ul><li>Th, 2.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6315">Registration</a></li></ul>This interactive session revisits essential features, shortcuts, and practical techniques in Empower software that can streamline everyday laboratory work and reduce unnecessary effort.<h5>5. Waters Corporation: Unlocking Therapeutic Peptide Characterisation (GLP-1RAs) with Cyclic IMS</h5><ul><li>Th, 2.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6313">Registration</a></li></ul>Discover how cyclic ion mobility spectrometry (cyclic IMS) improves characterization of GLP-1 analogues and therapeutic peptides, supporting development, quality control, and regulatory submissions.<h5>6. Agilent Technologies: Spectral Flow Cytometry Masterclass - Session 1</h5><ul><li>Fr, 3.7.2026 07:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6316">Registration</a></li></ul>Learn how full-spectrum flow cytometry enables high-dimensional cell analysis. Explore spectral flow technology, experimental design, and applications in immunology, oncology, and vaccine research.

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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DataApex Clarity Offline Chromatography Software

Clarity Offline allows users to import previously acquired data and to evaluate and prepare methods in regulated environment.

Clarity Offline allows users to import previously acquired data and to evaluate and prepare methods. This version cannot acquire data, but it can share data or even directly access (through LAN) projects of the <a href="https://lcms.labrulez.com/products/999">Clarity</a> and Clarity Lite software. With Clarity Offline users are able to work with acquired data on additional computers in the lab or at home. The software works with the same files and provides the same outputs (printing and exporting) as Clarity and Clarity Lite.
Clarity Offline is intended for use as additional software that supplements Clarity and Clarity Lite. It can also be used to evaluate data from other manufacturers&#39; CDS.
<blockquote>
Ask for <a href="https://www.dataapex.com/product/clarity-demo">Clarity Demo version</a> which allows you to get your hands on software before purchase
</blockquote>
<h3>Main features</h3>
<ul>
<li>21 CFR Part 11 compliance: Clarity Offline contains all functions (Electronic Signatures, User Accounts, Audit Trail, etc.) for compliance with the 21 CFR Part 11 directive of the FDA. Clarity Offline can be used for evaluation in regulated environments.
</li>
<li>Networked Solution: Clarity Offline can access files of Clarity and Clarity Lite installed on networked computers. This enables evaluation of chromatograms, development of new methods, and printing reports on additional computers in the laboratory.
</li>
<li>Education: An additional field of use is in education. Students can process chromatograms that have been acquired from Clarity connected to a chromatograph using their Clarity Offline license. Thus they do not need a Clarity full license just for data evaluation.
</li>
<li>Data processing and evaluation from other chromatography software: Clarity Offline allows the import of data from other chromatography software using text or AIA import function. This allows the user to process and evaluate chromatograms acquired by other chromatography software.
</li>
<li>Offsite computer: Clarity Offline can be installed on any Windows computer, giving you anytime access to your chromatographic data (USB hardware key is required).
</li>
<li>Language Localizations available: Useful for non-English speakers. At the moment, there are French, German, Russian, Spanish, and Chinese localizations available in a single installation package. Simply switch to your preferred language anytime.
</li>
</ul>
<a href="https://www.dataapex.com/controls">List of supported instruments</a>
<a href="https://www.dataapex.com/product/clarity-std-compatibility">Compatible Windows OS and Hardware</a>
<a href="https://www.dataapex.com/product/clarity-21cfr-compliance">Clarity 21 CFR Part 11 compliance</a>

Thermo Scientific Vanquish Horizon UHPLC System

Designed with innovative technology and fine detail, the Vanquish Horizon UHPLC delivers outstanding precision, sensitivity, and simplicity for unmatched performance and robustness.

Thermo Scientific Dionex AutoTrace 280 Solid-Phase Extraction Instrument

Extract analytes of interest from large volume aqueous samples easily and efficiently with the Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction (SPE) Instrument.

Extract analytes of interest from large volume aqueous samples easily and efficiently with the Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction (SPE) Instrument.&nbsp;The AutoTrace 280 automates all four steps of SPE (conditioning, loading, rinsing, and eluting), reducing solvent consumption and improving recovery and reproducibility. Boost productivity and reduce cost of analysis by simultaneously processing up to six samples in 2-3 hours with only 15 minutes of operator involvement. The AutoTrace 280 meets requirements in US EPA methods including the 500 and 600 series.<h5>Improved reliability and recovery</h5><ul><li>Automated SPE minimizes operator intervention</li><li>Positive pressure loading and elution of samples and solvents provide constant flow for improved analytical precision</li></ul><h5>Flexibility in operation</h5><ul><li>Loads sample volumes from 20 mL to 4 L</li><li>Compatible with 1, 3, and 6 mL cartridges and with 47 mm SPE disks</li><li>Automatically conditions, rinses, and elutes SPE cartridges with a choice of five solvents</li><li>Offers a choice of six different collection vial racks</li></ul><h5>Improved laboratory safety</h5><ul><li>Minimal sample handling reduces operator exposure to hazardous chemicals</li><li>No hood required</li></ul><h5>Enhanced laboratory productivity and reduced costs</h5><ul><li>Unattended operation</li><li>Batch processing of up to 6 samples</li><li>Reduced solvent usage</li><li>Segregates aqueous and solvent waste using separate waste lines for efficient, cost-effective waste handling</li></ul><h5>Supports a range of matrices and applications</h5><ul><li>Ability to process samples containing suspended solids</li><li>Meets requirements for solid-phase extraction for U.S. EPA methods including 500 and 600 series</li></ul><h5>Recommended use</h5><ul><li>Analyze prepared samples with Thermo Scientific LC or GC systems for a fully integrated workflow</li></ul><h4>Specifications</h4><ul><li>Instrument Type: Automated Solid Phase Extraction (SPE) System</li><li>Application: For use with 47 mm SPE disks</li><li>Model Description: AutoTrace 280 SPE System</li></ul><h5>Performance &amp; Operating Details</h5><ul><li>Disk Compatibility: Designed for 47 mm SPE disks</li><li>Pressure Range:<ul><li>Output: 0 to 30 psi (0 to 1.4 bar)</li><li>Input: Up to 100 psi (6.9 bar) maximum</li></ul></li><li>Voltage: 100–240 V ±10%</li><li>Frequency: 47–63 Hz</li></ul><h5>Physical Specifications</h5><ul><li>Dimensions (D × W × H): 57 × 63.5 × 69 cm</li><li>Unit Size: Each</li></ul>

Thermo Scientific EXTREVA ASE Accelerated Solvent Extractor

Achieve true walkaway sample-to-vial sample preparation with the Thermo Scientific™ EXTREVA™ ASE™ Accelerated Solvent Extractor. 

Achieve true walkaway sample-to-vial sample preparation with the Thermo Scientific™ EXTREVA™ ASE™ Accelerated Solvent Extractor. Without any intervention, perform sample extraction, in-cell cleanup and evaporation—all in one seamless operation—reducing manual labor from hours to minutes and enabling you to focus on other lab priorities while using up to 50% less solvent than other sample preparation techniques.Accelerate your journey from sample to vial<h5>The EXTREVA ASE Accelerated Solvent Extractor offers:</h5><ul><li>All-in-one sample preparation. The EXTREVA ASE system automatically extracts and concentrates samples, eliminating the need to manually move sample extracts to another device for solvent evaporation.</li><li>A walk-away solution. The system minimizes hands-on sample preparation tasks, enabling staff to accomplish other tasks.</li><li>Parallel extraction. One EXTREVA ASE system can perform four parallel sample extractions, substantially increasing throughput.</li><li>Gas-assisted extraction. The dynamic gas-assisted extraction mode reduces solvent consumption to between 5 and 100 milliliters per sample in most cases.</li><li>Automation. Full automation improves recoveries and reproducibility while reducing training needs. Unlike humans, automated systems are ideal for performing the same tedious task repeatedly, and they don’t make mistakes.</li><li>Method flexibility and optimization. Up to six different solvents can be used, including wash solvents for cleaning between methods. Precise flow control optimizes methods.</li><li>Barcode-reading. The system tracks sample information and preparation parameters using a 2-D bar code reader to facilitate tracking and integrity. Data is recorded on the instrument or Thermo Scientific™ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon™ Chromatography Data System (CDS) software</a>.</li></ul><h4>Specifications</h4><ul><li>Instrument Type: Automated Sample Preparation System</li><li>Application: Simultaneous sample extraction and concentration</li><li>Model Description: EXTREVA ASE Extraction and Evaporation System</li></ul><h5>Performance &amp; Capabilities</h5><ul><li>Extraction Temperature Range: 40–200 °C</li><li>Evaporation Temperature Range: 40–100 °C</li><li>Compatible Solvents: Wide range of organic and aqueous solvents</li><li>Pressure Capability: Up to 200 psi</li><li>Power Consumption: 2000 W</li><li>Frequency: 50/60 Hz</li></ul><h5>Sample &amp; Solvent Handling</h5><ul><li>Extraction Cells (stainless steel): 1, 5, 10, 22, 34, 66, 100 mL</li><li>Extraction Cell Tray: 16-position with 1 rinse</li><li>Collection Vials:<ul><li>60 mL and 100 mL evaporation vials</li><li>250 mL evaporation bottles</li><li>Direct concentrate to 2 mL sample vials with rinse capability</li></ul></li><li>Collection Vial Tray: 16-position tray insert for 60 mL vials and 250 mL bottles; includes 4 rinse positions</li></ul><h5>Utilities &amp; Requirements</h5><ul><li>Pneumatic Requirements:<ul><li>Air: 130–140 psi</li><li>Nitrogen: 350–450 psi</li></ul></li></ul><h5>Physical Specifications</h5><ul><li>Dimensions (D × W × H): 65.2 × 100 × 88.6 cm (25.6 × 39.4 × 34.8 in.)</li><li>Weight: 160 kg (353 lb)</li><li>Unit Size: Each</li></ul>

Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor

Save time, reduce solvent consumption, and boost productivity with Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor.

<div>Automate extraction, filtration and clean up of compounds from solid and semisolid samples in minutes using the automated Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor. This system accommodates sample sizes of 1-100g, allows unattended extraction of up to 24 samples and uses 50 to 90% less solvent compared to other methods.&nbsp;The chemically inert pathway supports acid and alkaline sample matrices and solvents. Flexible, easy-to-operate and cost-effective, the Dionex ASE 350 is ideally suited for high-throughput laboratories and for a wide variety of applications.<div><h4>Rapid and efficient extractions</h4><ul><li>Elevated temperature (up to 200°C) and pressure (1500psi) reduce extraction times and solvent consumption</li><li>Fast pump (70mL/min.) enables fast extractions (typically 12-20 minutes)</li><li>Specialized oven design ensures precise temperature control for excellent reproducibility</li><li>Integrated solvent controller allows mixing and delivery of up to three solvents</li><li>The Thermo Scientific™ SmartRun™ System ensures cell size and collection vessel size are matched for an optimal extraction</li></ul><h4>Enhanced flexibility to support multiple applications</h4><ul><li>System accommodates 1, 5, 10, 22, 34, 66 and 100mL cell sizes</li><li>pH-hardened pathway with Thermo Scientific™ Dionium™ components and cell enables compatibility with acid or alkaline pretreated matrices</li></ul><h4>Dramatically reduced solvent consumption</h4><ul><li>Requires less than 50mL solvent for a 20g sample</li><li>Includes solvent saver mode for further solvent savings</li></ul><h4>Enhanced laboratory productivity</h4><ul><li>System holds up to 24 samples for unattended operation</li><li>Flow-through technology allows in-line filtration and in-cell cleanup</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Dionex_ASE_350_system_2_9426ff3f9a/Thermo-Scientific-Dionex-ASE-350-system_2.jpg" alt="Thermo Scientific™ Dionex™ ASE™ 350 Accelerated Solvent Extractor" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Dionex_ASE_350_system_2_9426ff3f9a/thumbnail_Thermo-Scientific-Dionex-ASE-350-system_2.jpg 114w," sizes="100vw" width="579" height="792"><h3>🛠️ Specifications</h3><ul><li>Collection Vial Tray: 26-position tray insert for 60 mL vials; 19-position tray insert for 250 mL bottles</li><li>Depth (English): 27.3 in.</li><li>Depth (Metric): 69.3 cm</li><li>Description: Extractor Only</li><li>Extraction Fluids: Compatible with a wide range of organic and aqueous solvents</li><li>Fluid Sensors: IR</li><li>Frequency: 50/60 Hz</li><li>Height (English): 24.3 in.</li><li>Height (Metric): 61.7 cm</li><li>Pneumatic Requirements: Air at 400–827 kPa (60–120 psi); N2 (at 1034 ×–1340 kPa (150–200 psi))</li><li>Power Consumption: 500 Max VA</li><li>Pressure: 10 MPa (1500 psi)</li><li>Pump Flow: 70 mL/min</li><li>Temperature Control: Up to 200 °C</li><li>Weight (English): 140 lb.</li><li>Weight (Metric): 65 kg</li><li>Width (English): 26.5 in.</li><li>Width (Metric): 67.3 cm</li><li>Collection Vials: 60, 250 mL</li><li>Dimensions (D x W x H): 69.3 x 67.3 x 61.7 cm</li><li>Extraction Cell Tray: 24 Cell, 2 Rinse</li><li>Extraction Cells: 1, 5, 10, 22, 34, 66, 100 mL</li><li>For Use With (Equipment): Dionex ASE 350</li><li>Type: Dionex ASE 350 System</li><li>Unit Size: Each</li></ul></div></div>

Thermo Scientific Orbitrap Ascend Tribrid Mass Spectrometer

Be ready for tomorrow’s most challenging demands. Exceed experimental expectation with the MultiOmics, Structural Biology, and BioPharma Editions of the Thermo Scientific Orbitrap Ascend Editions Tribrid Mass Spectrometer.

Exceed experimental expectation with the MultiOmics, Structural Biology, and BioPharma Editions of the Thermo Scientific Orbitrap Ascend Editions Tribrid Mass Spectrometer.With new capabilities for multiplexed quantitative proteomics, native protein characterization, and deep biopharmaceutical analyses, the three new Editions deliver ultimate experimental throughput, versatility, and ease-of-use for your specific needs.Detect and characterize the most difficult protein complexes and native samples without losing low m/z instrument performance and flexibility with the new Native MS option, including world class Orbitrap detection up to m/z 16,000 and ion isolation in the ion trap and quadrupole up to m/z 8,000, with isolation widths down to m/z 100 and m/z 5, respectively.<figure class="image"><img style="aspect-ratio:1937/796;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Orbitrap_Ascend_Tribrid_hmotnostni_spektrometr_3_03576fcf9a/Thermo-Scientific-Orbitrap-Ascend-Tribrid-hmotnostni-spektrometr_3.jpg" alt="Thermo Fisher Scientific: Key innovations for Thermo Scientific Orbitrap Ascend Tribrid Mass Spectrometer" width="1937" height="796"></figure><h3>Orbitrap Ascend Editions Tribrid MS</h3><div><ul><li>Orbitrap Ascend MultiOmics Tribrid MS: Discover more from comprehensive metabolomics to proteomics.</li><li>Orbitrap Ascend Structural Biology Tribrid MS: Take advantage of ultimate versatility for native protein characterization.</li><li>Orbitrap Ascend BioPharma Tribrid MS: Successfully characterize your most complex biotherapeutics.</li></ul><div><h3>Introducing the Orbitrap Ascend Editions Tribrid MS</h3></div><div><div><div id="container-deb530bb93"><div><div><div id="container-fffbeb2334"><div><div><div id="text-1451003751">Eliminate the guesswork from your purchase decision with the MultiOmics, BioPharma, and Structural Biology Editions of the Thermo Scientific Orbitrap Ascend Tribrid Mass Spectrometer.&nbsp;Simplify your decisions and streamline your research with a tailored configuration for your unique application space.Employ cutting edge native structural and biopharmaceutical analyses with multiple fragmentation and ion manipulation techniques. With the new Native MS option, detect protein complexes up to m/z 16,000 in the Orbitrap mass analyzer and isolate proteoforms&nbsp;up to m/z 8,000 with an isolation width as low as m/z 5 in the quadrupole.Quantify more peptides, identify more proteins, confidently assign more labile-PTMs, and discover more previously undetectable protein complexes. Characterize proteoforms and modified compounds with more precision than ever before.&nbsp;</div></div></div></div></div></div></div></div></div><h3>System highlights</h3><h5>Spend less time on instrument set-up and focus on your science</h5>With the integrated Auto-Ready ion source, trigger on-demand or scheduled system calibrations remotely and without disruption to your LC-MS system.<h5>Gain increased characterization from lipids to intact proteins to oligonucleotides with orthogonal fragmentation &nbsp;</h5>The Orbitrap Ascend Editions mass spectrometers have multiple fragmentation techniques that provide complementary information.<ul><li>Standard collision-induced dissociation (CID) and higher-energy collisional dissociation (HCD) can be used for multiple analytes.</li><li>Optional electron transfer dissociation (ETD) increases coverage for proteins and glycopeptide experiments, and ultraviolet photodissociation (UVPD) benefits protein, oligonucleotide, and lipid analysis.</li></ul><h5>Study the largest protein complexes using native MS techniques, all on the same system as bottom-up proteomics techniques</h5>The Native MS option comprises the extension of the Orbitrap mass range to m/z 16,000, ion trap ion isolation up to 8,000 with MSn capabilities, and more sensitive and selective extended quadrupole isolation up to m/z 8,000. With the Native MS option, you can detect large protein complexes and characterize the subunits by multiple fragmentation techniques.<h5>Environmentally friendly dry pumps decrease energy usage and maintenance</h5>By changing the fore pumps from oil-based to a dry pump, we reduce the heat output by 22%. They also eliminate the production of oil vapors and the need for oil checks and changes, lowering maintenance.The Orbitrap Ascend Editions mass spectrometers have also received the ACT label, the standard for determining the environmental impact of laboratory products to help labs make informed, sustainable purchasing decisions.<h5>Software solutions for your application</h5>Streamlined instrument control software across all next-generation Thermo Scientific platforms for ease-of-use through different systems. Edition-specific application templates are ready to use and built into the method editor so you can get meaningful results faster.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Thermo_Scientific_Orbitrap_Ascend_Tribrid_Mass_Spectrometer_The_most_versatile_platform_for_deep_untargeted_and_accurate_targeted_metabolomics_336c0b833d.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Orbitrap Ascend Tribrid Mass Spectrometer - The most versatile platform for deep untargeted and accurate targeted metabolomics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Thermo_Scientific_Orbitrap_Ascend_Tribrid_Mass_Spectrometer_The_most_versatile_platform_for_deep_untargeted_and_accurate_targeted_metabolomics_336c0b833d.jpg 245w," sizes="100vw" width="2349" height="1165"><h4>Orbitrap Ascend MultiOmics Tribrid MS</h4>Benefit from experimental throughput, versatility, and ease-of-use. With industry leading capabilities, the Thermo Scientific Orbitrap Ascend MultiOmics Tribrid Mass Spectrometer delivers ultimate data quality. Quantify more samples at lower concentrations using intelligent acquisition and faster Real-Time Search.<h5>Applications</h5><ul><li>The most versatile platform for deep untargeted and accurate targeted metabolomics</li><li>The most versatile platform for deep untargeted and accurate targeted metabolomics</li><li>The most versatile platform for deep untargeted and accurate targeted metabolomics</li><li>The most versatile platform for deep untargeted and accurate targeted metabolomics</li><li>The most versatile platform for deep untargeted and accurate targeted metabolomics</li><li>Lipidomics experiments benefit from AqcuireX software and alternative fragmentation capabilities</li></ul><h4>Orbitrap Ascend Structural Biology Tribrid MS</h4><div><div id="container-26e6c6fa34"><div><div><div id="text-872767366">Be prepared for tomorrow's challenges in structural analysis with this powerful solution. Achieve greater coverage and explore different ion manipulation options with an expanded m/z range and multiple fragmentation options.<ul><li>Characterize complex molecules (Native MS option) with a recommended mass range up to m/z 16,000 and an extended quadrupole isolation range of up to m/z 8,000.</li><li>Take advantage of multiple fragmentation options (ETD, EThcD, UVPD) and Proton-Transfer Charge Reduction to achieve comprehensive sequence coverage of your protein target.</li></ul><h5>Applications</h5><ul><li>Native MS option facilitates the identification of membrane proteins by top-down MS&nbsp;</li><li>Utilizing PTCR for complex mixture analysis enables more identification across the m/z range &nbsp;</li><li>Crosslinking experimentation is aided by Real-Time Library Search available on the Orbitrap Ascend Structural Biology MS&nbsp;&nbsp;</li><li>Comprehensive disulfide bond mapping analysis using FAIMS and EThcD</li><li>Characterize larger proteoforms by top-down proteomics</li><li>Characterize larger proteoforms by top-down proteomics</li><li>Native MS option extends ion detection of large protein complexes</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Thermo_Scientific_Orbitrap_Ascend_Tribrid_Mass_Spectrometer_Lipidomics_experiments_benefit_from_Aqcuire_X_software_and_alternative_fragmentation_capabilities_da54862143.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Orbitrap Ascend Tribrid Mass Spectrometer - Lipidomics experiments benefit from AqcuireX software and alternative fragmentation capabilities" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Thermo_Scientific_Orbitrap_Ascend_Tribrid_Mass_Spectrometer_Lipidomics_experiments_benefit_from_Aqcuire_X_software_and_alternative_fragmentation_capabilities_da54862143.jpg 245w," sizes="100vw" width="3257" height="850"><h4>Orbitrap Ascend BioPharma Tribrid MS</h4></div></div></div></div></div>Elevate your analytical capabilities and successfully characterize your most complex products from peptides to native protein characterization with the Thermo Scientific Orbitrap Ascend BioPharma Tribrid Mass Spectrometer.&nbsp; Achieve greater coverage for oligonucleotide sequences and analyze even the largest and most complex molecules.<ul><li>Recommended alternate fragmentation techniques enable detailed structural elucidation.</li><li>Automated Auto-Ready ion source and application-specific pre-built method templates streamline instrument setup.</li></ul><h5>Applications</h5><ul><li>Comprehensive native analysis of complex therapeutic proteins using Native MS option and PTCR</li><li>Comprehensively characterize antibody-drug conjugates combining orthogonal fragmentation techniques</li><li>Enhanced speed and sensitivity for high sequence coverage and low abundant PTM identifications in peptide mapping experiments</li><li>Improved sensitivity allows highly confident identification of ppb Host Cell Proteins at analytical flow rates</li><li>Characterize intact sgRNA up to 100 base pairs using low-q CID fragmentation</li><li>Unparalleled characterization of low-abundant modifications in oligonucleotides</li></ul>&nbsp;</div>

Thermo Scientific Orbitrap Astral Mass Spectrometer

A new horizon for single-cell, quantitative, and translational proteomics.

<div><div container-id="cq-colctrl-lt1" comp-id="A1"><div>Looking to unlock additional insights into biology and disease mechanisms? With faster throughput, deeper coverage, higher sensitivity, and accurate and precise quantitation; the Thermo Scientific Orbitrap Astral Mass Spectrometer enables you to dramatically expand the scale and scope of your experiments.See how it sets a new standard for mass spectrometry performance and redefines what is possible for discovery and translational research to help you rapidly and comprehensively capture the dynamic, temporal, and spatial complexity of biology.<h3>Key benefits</h3><h4>Faster throughput</h4>Achieve faster throughput with a high throughput injection-to-injection cycle of just 8 minutes.<ul><li>Analyze one sample in only 8 minutes</li><li>In one day, analyze over 1.4 million protein groups from 180 samples</li></ul><h4>Deeper coverage</h4>The near whole-proteome coverage of these methods reduces the chances of missing out on important proteins of interest.<ul><li>See more in less time or spend more time and go even deeper</li><li>Double the depth and quadruple the throughput for phosphoproteomics</li></ul><h4>Higher sensitivity</h4>The high dynamic range of the proteome necessitates the measurement of not only high abundance proteins, but also proteins with just a few copies.<ul><li>Deeper coverage from single cells at faster throughput</li><li>1 ng HeLa DIA/18 min (80 SPD)</li></ul><h4>Accurate and precise quantitation</h4>Biological changes are quantitative in nature, requiring accurate and precise measurements to determine statistically significant biomarkers.<ul><li>Accurate and precise quantitation of three-proteome mixtures</li><li>The Orbitrap Astral mass spectrometer enables accurate and precise quantitation with 88% of identified proteins having 20% CV or less and 75% having 10% CV or less</li></ul><img style="aspect-ratio:1224/513;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Konstrukce_hmotnostniho_spektrometru_Thermo_Orbitrap_Astral_e38e10580c/Pragolab-Konstrukce-hmotnostniho-spektrometru-Thermo-Orbitrap-Astral.jpg" alt="Thermo Fisher Scientific: Thermo Scientific Orbitrap Astral Mass Spectrometer" width="1224" height="513"><h3><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer.html">An overview</a> of the Orbitrap Astral MS from Alexander Makarov, Ph.D.</h3>The new Astral analyzer builds on Thermo Fisher Scientific’s proven Orbitrap mass spectrometry platform with novel technology to deliver up to two times deeper proteome coverage and up to four times more throughput compared to current mass spectrometers.Alexander Makarov, Ph.D., Director Global Research LSMS, Thermo Fisher Scientific, claims the Astral analyzer represents a new class of high-resolution accurate-mass analyzers with a unique performance envelope."The introduction of the Astral analyzer represents a starting point in the future evolution of its technology to new frontiers of performance," said Makarov.<h3>What customers are saying</h3><h4>A New Standard for Data-Independent Acquisition</h4>Learn how the Orbitrap Astral mass spectrometer will enable precision medicine at scale with revolutionary new DIA methods for plasma proteomics.The future of proteomics lies in the accurate and precise measurement of thousands of proteins across thousands of samples. Data-independent Acquisition (DIA) facilitates deep proteome coverage with high quantitative accuracy and precision using label-free quantitation (LFQ) due to the unbiased and reproducible nature of mass spectrometry measurements.<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331001711112">Mike MacCoss, Ph.D.</a>, Professor, Department of Genome Sciences, University of Washington</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331014977112">Jennifer Van Eyk, Ph.D.</a>, Director, Basic Science Research, Barbra Streisand Women’s Heart Center Director, Advanced Clinical Biosystem Institute Center, Cedars-Sinai</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331000425112">Jesper Olsen, Ph.D.</a>, Deputy Center Director, Professor, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331001022112">Qing Yu</a>, Research Associate at Harvard Medical School</li></ul></div></div></div><h4>The Era of Single-Cell Proteomics Has Arrived</h4>Single cell proteomics can determine the dynamic nature of the proteome across cell types in both time and space. The increased resolution that is gained by measuring single cells instead of bulk samples provides a deeper insight into disease mechanisms and fundamental biological processes to unravel the complexity of biology. Learn how the Orbitrap Astral mass spectrometer opens a new horizon for single-cell proteomics by empowering higher throughput and deeper coverage without sacrificing quantitative accuracy and precision.<div><div container-id="cq-colctrl-lt0" comp-id="B1"><div><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331010858112">Erwin Schoof, Ph.D.</a>, Associate Professor, Department of Biotechnology and Biomedicine, Section for Protein Science and Biotherapeutics, Cell Diversity Lab, Technical University of Denmark</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331001916112">Joshua Coon, Ph.D.</a>, Professor of Biomolecular Chemistry at the University of Wisconsin-Madison</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331012816112">Nikolai Slavov, Ph.D.</a>, Founding Director of Parallel Squared Technology Institute and Professor at Northeastern University</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-astral-mass-spectrometer/why-orbitrap-astral.html?videoId=6331016836112">Sophia Steigerwald, Ph.D.</a>, Student at the Max Planck Institute of Biochemistry</li></ul></div></div></div><h3>How it works</h3>The Orbitrap Astral Mass Spectrometer combines three mass analyzers: a quadrupole mass analyzer for high selectivity and high ion transmission; an Orbitrap mass analyzer for high dynamic range and high-resolution measurements; and a novel Astral analyzer for fast and sensitive measurements. The front end, from the ion source up to the segmented quadrupole mass filter, is designed to maximize instrument sensitivity and robustness. Ions are trapped and concentrated in the ion routing multipole, which facilitates the transfer of ions to either of the high-resolution accurate-mass (HRAM) analyzers. Synchronization of ion transfer and processing throughout the instrument enables the parallel handling of five separate ion packets simultaneously. This synchronization enables each mass analyzer to be used in combination to optimize performance and experimental flexibility.The Orbitrap analyzer is ideally suited for the acquisition of high dynamic range HRAM MS and MS2 spectra, including an optional Biopharma mode to extend the mass range up to m/z 8,000. The Astral Mass Analyzer delivers high acquisition rates up to 200 Hz, single ion detection sensitivity, and high dynamic range HRAM SIM and MS2 measurements that are fully synchronized with parallel Orbitrap analyzer operation. Due to this synchronization the Orbitrap Astral Mass Spectrometer excels at many data acquisition strategies including High Resolution Data-Independent Acquisition (HR-DIA) and data-dependent acquisition (DDA). These acquisition strategies combined with the high resolution and dynamic range of the Orbitrap and Astral analyzers enable accurate and precise quantitation using either Label-Free Quantitation (LFQ) or multiplexing with Tandem Mass Tag (TMT)-labeled peptides.</div></div></div>A 3D tour of the instrument can be found <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/virtual/orbitrap-astral-ms-3d-tour.html">here</a>.

Advanced PFAS Analytical Services (ALS)

Comprehensive PFAS analysis in water, soil, sludge, and AFFF sites using LC-MS/MS. Detect 60+ PFAS compounds, meet EU limits, and ensure compliance with evolving 2025 regulations.

<h4>Advanced PFAS Analytical Services</h4><h5>Ensuring Compliance, Safety and Environmental Responsibility</h5>Per- and polyfluoroalkyl substances (PFAS) are a group of synthetic chemicals widely used in industrial applications and consumer products. Their persistence in the environment and potential health risks have led to increasing regulatory scrutiny, particularly in drinking water and firefighting foam residues.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Advanced_PFAS_Analytical_Services_c8d8b28b7c.jpg" alt="ALS: Advanced PFAS Analytical Services" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Advanced_PFAS_Analytical_Services_c8d8b28b7c.jpg 245w," sizes="100vw" width="1604" height="502"><h4>Our Expertise</h4>We offer state-of-the-art analytical services for the detection and quantification of PFAS in:<ul><li>Drinking/ground/surface/waste water</li><li>Soil, sediment, sludge and biosolids</li><li>AFFF-contaminated sites and products</li></ul><h5>Using LC-MS/MS, our laboratories provide:</h5><ul><li>Ultra-trace level detection (ng range)</li><li>Analysis of over <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/enviromail_01_europe_the_scope_of_pfas_analyses_in_waters_and_current_state_of_legislation_6ebd4f1b6f/enviromail_01_europe_the-scope-of-pfas-analyses-in-waters-and-current-state-of-legislation.pdf">60 individual PFAS compounds</a></li><li>Non-targeted PFAS analysis like TOPA</li></ul><blockquote><h5>Contact Us</h5>Let us help you navigate the complex landscape of PFAS regulation and ensure your operations meet the highest standards of safety and compliance.<ul><li>+420 226 226 228</li><li><a href="mailto:customer.support@alsglobal.com">customer.support@alsglobal.com</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.alsglobal.eu/">alsglobal.eu</a></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_PFAS_421ef75bea.jpg" alt="ALS: PFAS Analytical Services" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_PFAS_421ef75bea.jpg 156w," sizes="100vw" width="919" height="919"></blockquote><h4>Regulatory Context in Europe (2025)</h4>European legislation is rapidly evolving to address PFAS contamination:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://eur-lex.europa.eu/legal-content/EN/LSU/?uri=oj:JOL_2020_435_R_0001">The EU Drinking Water Directive (2020/2184) </a>mandates monitoring of PFAS, with a parametric value of 0.10 μg/L for the sum of 20 PFAS compounds</li><li><a target="_blank" rel="noopener noreferrer" href="https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=OJ:L:2006:396:TOC">Reach Regulation 1907/2006</a> and the key <a target="_blank" rel="noopener noreferrer" href="https://eur-lex.europa.eu/eli/reg/2021/1297/oj/eng">Amendment Regulation 2021/1297</a>, targeting the C9-C14 perfluorocarboxylic acids and related substances</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.consilium.europa.eu/en/press/press-releases/2025/04/10/soil-monitoring-law-council-reaches-deal-with-parliament/">The Soil Monitoring law</a> (provisional agreement april 2025) includes PFAS as part of the soil Health assessment</li><li><a target="_blank" rel="noopener noreferrer" href="https://environment.ec.europa.eu/topics/water/water-framework-directive_en">The Water Frame Directive </a>with EQS on surface and groundwater and considerations for sediments and biota</li></ul><h3>List of PFAS analytes and report limits for determining PFAS substances in water</h3>For the sum of PFAS, a limit of 0.1 μg/l has been set, which applies to 20 substances marked with an asterisk (*) in the text below.<h5>Group: Perfluoroalkyl carboxylic acids</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>Perfluorobutanoic acid / PFBA* / 0.01 / 0.002</li><li>Perfluoro-3-methoxypropanoic acid / PFMPA / 0.025 / 0.001</li><li>Perfluoropentanoic acid / PFPeA* / 0.01 / 0.0003</li><li>Perfluoro-4-methoxybutanoic acid / PFMBA / 0.025 / 0.001</li><li>Perfluorohexanoic acid / PFHxA* / 0.01 / 0.0003</li><li>Tetrafluoro-2-(heptafluoropropoxy) propanoic acid / HFPO-DA / 0.02 / 0.001</li><li>Perfluoroheptanoic acid / PFHpA* / 0.01 / 0.0003</li><li>4,8-dioxa-3H-perfluorononanoic acid / DONA / 0.01 / 0.002</li><li>7H-perfluoroheptanoic acid / HPFHpA / 0.01 / 0.001</li><li>Perfluorooctanoic acid / PFOA* / 0.005 / 0.0003</li><li>Perfluoro-3,7-dimethyloctanoic acid / P37DMOA / 0.01 / 0.001</li><li>Perfluorononanoic acid / PFNA* / 0.01 / 0.0003</li><li>Perfluorodecanoic acid / PFDA* / 0.01 / 0.0003</li><li>2H,2H,3H,3H-Perfluorooctanoic acid / H4PFUnDA / 0.02 / 0.0003</li><li>Perfluorooctanoic acid / PFUnDA* / 0.01 / 0.0003</li><li>Perfluorododecanoic acid / PFDoDA* / 0.01 / 0.0003</li><li>Perfluorotridecanoic acid / PFTrDA* / 0.01 / 0.0003</li><li>Perfluorotetradecanoic acid / PFTeDA / 0.025 / 0.0003</li><li>Perfluorohexadecanoic acid / PFHxDA / 0.05 / n.a.</li><li>Perfluorooctadecanoic acid / PFOcDA / 0.05 / n.a.</li></ul><h5>Group: Perfluoroalkylsulfonic acids</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>Perfluoropropanesulfonic acid / PFPrS / 0.02 / 0.001</li><li>Perfluoro(2-ethoxyethane)sulfonic acid / PFEESA / 0.1 / 0.001</li><li>Perfluorobutanesulfonic acid / PFBS* / 0.01 / 0.0003</li><li>Perfluoropentanesulfonic acid / PFPeS* / 0.01 / 0.0003</li><li>Perfluorohexanesulfonic acid / PFHxS* / 0.01 / 0.0003</li><li>Perfluoroheptanesulfonic acid / PFHpS* / 0.01 / 0.0003</li><li>Perfluorooctane sulfonic acid / PFOS* / 0.005 / 0.0003</li><li>n-Decafluoro-4-(pentafluoroethyl) cyclohexane sulfonic acid / PFECHS / 0.01 / 0.0003</li><li>Perfluorononane sulfonic acid / PFNS* / 0.01 / 0.0003</li><li>Perfluorodecane sulfonic acid / PFDS* / 0.01 / 0.0003</li><li>Perfluorooctadecane sulfonic acid / PFUnDS* / 0.01 / 0.001</li><li>Perfluorododecane sulfonic acid / PFDoDS* / 0.01 / 0.0003</li><li>Perfluorotridecane sulfonic acid / PFTrDS* / 0.02 / 0.001</li></ul><h5>Group: Fluorotelomeric sulfonates</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>4:2 fluorotelomeric sulfonate / 4:2 FTS / 0.01 / 0.0003</li><li>6:2 fluorotelomeric sulfonate / 6:2 FTS / 0.01 / 0.0003</li><li>8:2 fluorotelomeric sulfonate / 8:2 FTS / 0.01 / 0.0003</li><li>10:2 fluorotelomeric sulfonate / 10:2 FTS / 0.01 / n.a.</li></ul><h5>Group: Perfluorinated sulfonamides</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>N-methyl-perfluorooctanesulfonamide / MeFOSA / 0.05 / 0.002</li><li>N-ethyl perfluorooctane sulfonamide / EtFOSA / 0.05 / 0.002</li><li>N-methyl perfluorooctane sulfonamidoethanol / MeFOSE / 0.025 / 0.002</li><li>N-ethyl perfluorooctane sulfonamidoethanol / EtFOSE / 0.025 / 0.002</li></ul><h5>Group: Perfluorooctane sulfonamidoacetic acid derivatives</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>Perfluorooctane sulfonamidoacetic acid / FOSAA / 0.01 / 0.001</li><li>N-Methyl perfluorooctane sulfonamidoacetic acid / MeFOSAA / 0.01 / 0.001</li><li>N-Ethyl perfluorooctane sulfonamidoacetic acid / EtFOSAA / 0.01 / 0.001</li></ul><h5>Group: Fluorotelomeric carboxylic acids</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>3-Perfluoropropyl propanoic acid (3:3) / 3:3 FTCA / 0.1 / 0.0003</li><li>n-2-Perfluorohexyl ethanoic acid (6:2) / 6:2 FTCA / 0.1 / 0.001</li><li>3-Perfluoropentyl propanoic acid (5:3) / 5:3 FTCA / 0.02 / 0.001</li><li>2H-Perfluoro-2-octenoic acid (6:2) / 6:2 FTUCA / 0.02 / 0.001</li><li>3-Perfluoroheptyl propanoic acid (7:3) / 7:3 FTCA / 0.02 / 0.0003</li><li>n-2-Perfluorooctyl ethanoic acid (8:2) / 8:2 FTCA / 0.1 / 0.001</li><li>2H-Perfluoro-2-decanoic acid (8:2) / 8:2 FTUCA / 0.02 / 0.001</li></ul><h5>Group: Chlorinated perfluoroalkyl sulfonic acids</h5>Analyte / Abbreviation / Standard method (μg/l) / Low-limit method (μg/l)<ul><li>9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid / 9Cl-PF3ONS / 0.01 / 0.001</li><li>11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid / 11Cl-PF3OUdS / 0.01 / 0.002</li></ul>*Analytes included in the sum of 20 PFAS in drinking water according to DIRECTIVE (EU) 2020/2184 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 16 December 2020 on the quality of water intended for human consumption.<h4>Applications</h4><ul><li>Site investigations and remediation planning</li><li>Compliance monitoring for water utilities</li><li>Environmental due diligence for industrial sites</li><li>AFFF legacy contamination assessments</li><li>Products compliance</li></ul><h4>Why Choose Us?</h4><ul><li>Accredited laboratories with experience in regulatory, diagnostic and scientific PFAS analyses</li><li>Rapid turnaround times and customizable reporting formats</li><li>Expert support for laboratories, environmental consultants, utilities, and industrial clients</li><li>Webinars by specialists in the field</li></ul><blockquote><h5>Read more about PFAS analysis:</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/enviromail_02_europe_sampling_recommendations_for_pfas_to_maximize_data_quality_26c1da00bb/enviromail_02_europe_sampling-recommendations-for-pfas-to-maximize-data-quality.pdf">EnviroMail™ 02/Europe: Sampling Recommendations for PFAS to Maximize Data Quality</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/enviromail_10_europe_pfas_forever_chemicals_in_soil_a5b49ad7dd/enviromail_10_europe_pfas_forever-chemicals-in-soil.pdf">EnviroMail™ 10/Europe: PFAS: “Forever chemicals” in soil</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/enviromail_13_europe_passive_sampling_for_the_monitoring_of_pfas_in_water_feb2024_227a69e2dc.pdf">EnviroMail™ 13/Europe: Passive sampling for the monitoring of PFAS in water</a></li></ul></blockquote>

ARC Solvere Universal HPLC Detector

The Solvere™ is a new LC detector that brings the benefits of the Flame Ionization Detector (FID) to LC. 
Using ARC’s expertise in catalysis and reactor design, a novel method was developed for removing volatile compounds, which allows for the use of both aqueous and organic solvents with the detector. 
Non-volatile components in the sample stream are transformed into the gas phase, converted to methane, and then detected by the FID with a linear range of five orders of magnitude.
ARC Solvere is designed for quantitation of compounds where GC ends with a range up to large 1M+ Da molecules. In addition, Solvere works with a myriad of mobile phases, additives, and gradients, with unprecedented linear range, uniformity and robustness.
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ARC_Koncept_detekce_pomoci_HPLC_FID_detektoru_Solvere_1090d61827/ARC-Koncept-detekce-pomoci-HPLC-FID-detektoru-Solvere.jpg" alt="ARC: The concept of HPLC - FID detector Solvere"/>
<h3>Why is it important?</h3>
<ul>
<li>Mobile phases are effectively separated from non-volatile analytes</li>
<li>Analytes are converted into methane for universal FID detection</li>
<li>Compatible with gradients and organic solvents</li>
<li>A variety of molecules such as proteins, peptides, and sugars give a universal carbon response</li>
</ul>
<blockquote>
FOR USE WITH ANY LC MODEL
</blockquote>
<h3>Benefits of the Solvere</h3>
<ul>
<li>Analyte response independent of mobile phase composition</li>
<li>Conversion of analytes to methane for universal FID response</li>
<li>5 orders of magnitude range</li>
<li>~10 ppm LOD</li>
<li>Standalone and automated</li>
</ul>
<h3>Solvere - Universal FID for HPLC</h3>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ARC_Ukazka_detekce_Sacharozy_pomoci_Solvere_detektoru_pro_HPLC_21106ae2ae/ARC-Ukazka-detekce-Sacharozy-pomoci-Solvere-detektoru-pro-HPLC.jpg" alt="ARC: Example of Sacharose Analysis Using Solvere Detector for HPLC"/>

DataApex Clarity Chromatography Software

Clarity is a chromatography data software for data acquisition, processing, and instrument control in regulated environment. Clarity is a flexible solution for devices from different vendors, supporting different applications.

Optional control modules provide integrated control of selected instruments such as GCs, LCs, pumps, detectors, autosamplers, etc. Extensions provide functions for specific separation techniques or calculations such as PDA, GPC analysis, Mass spectrometry, or SST. Clarity can run in English, French, German, Russian, Spanish, and Chinese language. Support technicians and developers are close to their users and ready to provide extensive Support from DataApex.
<blockquote>
Ask for <a href="https://www.dataapex.com/product/clarity-demo">Clarity Demo version</a> which allows you to get your hands on software before purchase
</blockquote>
<h3>Main features</h3>
<ul>
<li>Measuring: Simultaneous data acquisition from up to four independent chromatographs, each chromatograph can acquire data from up to 32 detectors.
</li>
<li>Integration: There is an extensive possibility to modify chromatograms. The chromatogram can be changed by entering global parameters or interactively through direct graphic modification of the baseline.
</li>
<li>Overlay: Simultaneously displays a virtually unlimited number of chromatograms and their mathematical modification; for example, mutual deductions or derivations of any order.
</li>
<li>Calibration: Internal and external standard calculation methods, calibration of groups of peaks, and reference peaks method for better identification.
</li>
<li>Wide range of supported instruments: Control modules in Clarity allow users to control various instruments in their lab. See our growing <a href="https://www.dataapex.com/controls">List of supported instruments</a>.
</li>
<li>Extensions completing your workflow: Clarity Extensions integrates your workflow into one application. GPC calculations, PDA data processing, gas or detailed hydrocarbon analyses according to selected norms, and much more.
</li>
<li>Automated measuring support: Sequence tables for any set of samples with or without an autosampler.
</li>
<li>Postrun: Automatically displays, prints, exports, and starts other programs after the completion of a measurement.
</li>
<li>Summary result tables: Displays and prints selected results from all simultaneously displayed chromatograms.
</li>
<li>User settings: User selects parameters for peak display and the specification for axes, including color from an extensive array of color settings. Text labels and lines, either as part of the area or anchored to a chromatogram, may also be inserted.
</li>
<li>Export: Optional exportation of all results, with or without the chromatogram, in various formats, into a file or clipboard.
</li>
<li>Import: Imports chromatograms or mathematical curves, which have been saved in text or AIA formats, from other programs.
</li>
<li>LIMS: Clarity offers connectivity with LIMS, both for sample submission and result output. This can be done via convenient ASCII transfers.
</li>
<li>Method and calibration history: Each chromatogram can easily be displayed under the same conditions as when it was printed, exported, or saved.
</li>
<li>Column performance: Calculations of peaks in terms of symmetry, efficiency, resolution; all by several methods (tangent, moments, etc.).
</li>
<li>Batch: Automatically batch processes, displays, exports, or prints any number of chromatograms.
</li>
<li>User calculations: Users can define custom calculations in the Result and Summary tables. Using the integrated editor, you can create your own columns from original columns and individual mathematical functions.
</li>
<li>User accounts: Sets up access rights and passwords (including their parameters, e.g., minimum length, validity, etc.). Each user can define his or her own appearance of the software.
</li>
<li>Audit trail: Records selected events and operations into a special file. Records selected operations directly into a chromatogram.
</li>
<li>Electronic signature: Each chromatogram can be signed electronically. Signature selection is based on the username or the signature certificate.
</li>
<li>Networked Solution: Clarity chromatography software files can be accessed from networked computers using Clarity Offline software. This enables the offline evaluation of chromatograms, development of new methods, and printing reports. See the <a href="https://gcms.labrulez.com/products/1000">Clarity Offline</a> for more details.
</li>
<li>Language Localizations available: Useful for non-English speakers. At the moment, there are French, German, Russian, Spanish, and Chinese localizations available in a single installation package. Simply switch to your preferred language anytime.
</li>
<li>Competitive approach: The chromatography market offers many specialized HPLC data systems or GC data systems; these are often provided with the purchased instrument. It is usually complicated to integrate such software into the workflow of the laboratory since they do not enable direct control of additional instruments. Compared to other small-scale solutions, Clarity offers a wide range of directly controlled instruments as well as optional Extensions for specific separation techniques. On the other hand, complex solutions with a wide scope of features and scalable design are quite expensive, which is a limiting factor for many customers. Another drawback of the large solutions is their complexity which imposes high demands on users. Compared to this, Clarity represents a scalable medium-ranged solution with a steep learning curve and competitive prices.
</li>
</ul>
<h3>Clarity configurations examples</h3>
<h4>One chromatograph with two detectors</h4>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_Chromatograficky_Software_Clarity_configurations_examples_One_chromatograph_with_two_detectors_ae482b83b8/DataApex-Clarity-Chromatograficky-Software-Clarity-configurations-examples-One-chromatograph-with-two-detectors.jpg" alt="DataApex Clarity Chromatography Software: Clarity configurations examples - One chromatograph with two detectors.jpg"/>
<ul>
<li>The HPLC measures on two detectors. Clarity will acquire two separate signals sharing a common timebase (synchronized START/STOP). A resulting chromatogram will contain two signals.</li>
</ul>
<h4>Two chromatographs, one with multiple detectors</h4>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_Chromatograficky_Software_Clarity_configurations_examples_Two_chromatographs_one_with_multiple_detectors_b21dc07faf/DataApex-Clarity-Chromatograficky-Software-Clarity-configurations-examples-Two-chromatographs-one-with-multiple-detectors.jpg" alt="DataApex Clarity Chromatography Software: Clarity configurations examples - Two chromatographs, one with multiple detectors.jpg"/>
<ul>
<li>This example displays a configuration with two chromatography systems (GC + HPLC) where the HPLC system uses multiple detectors. All the detectors attached to the HPLC Instrument will share common timebase (synchronized START/STOP). The GC detector will be independent.</li>
</ul>
<h4>Two chromatographs, one digital control</h4>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_Chromatograficky_Software_Clarity_configurations_examples_Two_chromatographs_one_digital_control_1bf9cec334/DataApex-Clarity-Chromatograficky-Software-Clarity-configurations-examples-Two-chromatographs-one-digital-control.jpg" alt="DataApex Clarity Chromatography Software: Clarity configurations examples - Two chromatographs, one digital control.jpg"/> This example displays a configuration with two chromatography systems (GC + LC). The GC is connected and controlled via digital interface *, using a GC Control appropriate module.
<h4>Four chromatographs with autosampler each</h4>
<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_Chromatograficky_Software_Clarity_configurations_examples_Four_chromatographs_with_autosampler_each_945873d808/DataApex-Clarity-Chromatograficky-Software-Clarity-configurations-examples-Four-chromatographs-with-autosampler-each.jpg" alt="DataApex Clarity Chromatography Software: Clarity configurations examples - Four chromatographs with autosampler each.jpg"/>
<ul>
<li>This example displays a configuration with two chromatography systems (GC + LC). The GC is connected and controlled via digital interface *, using a GC Control appropriate module.</li>
</ul>
<a href="https://www.dataapex.com/controls">List of supported instruments</a>
<a href="https://www.dataapex.com/product/clarity-std-compatibility">Compatible Windows OS and Hardware</a>
<a href="https://www.dataapex.com/product/clarity-21cfr-compliance">Clarity 21 CFR Part 11 compliance</a>

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