In closed communities such as the Amish in North America, rare genetic disorders occur more frequently. In children from this community, a condition has been described that affects the development of the nervous system and manifests, for example, as muscle weakness or epileptic seizures. The disorder is linked to a small change in a single protein, but for a long time it was unclear which cellular process this change disrupts. This question has now been answered by researchers from CEITEC Masaryk University, who show that the disease is caused by a disruption of a key mechanism required for the proper processing of genetic information in the cell.Every cell must first transcribe genetic information and then modify it before it can be used. From the original “working transcript,” unnecessary parts are removed and the remaining pieces are precisely joined together. This process, known as RNA splicing, consists of many consecutive steps and is tightly regulated. If the process does not proceed correctly or stops at any point, the cell cannot properly convert the copied genetic information into a usable form and therefore cannot function as it should.Researchers from CEITEC Masaryk University have now described one of the key steps in this process – a specific signal inside the cell. This signal acts as a checkpoint: only once it is fulfilled, the entire mechanism is activated and the process can continue.“We showed that during RNA splicing, a chemical modification of the protein SF3B1 is required at a precisely timed step. This modification allows another protein, SNIP1, to bind. If this signal is not transmitted correctly, the cell cannot proceed with the splicing process,” explains first author <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/pavla-gajduskova-ph-d/u26136?page=publication">Pavla Gajdušková</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Vzacna_geneticka_porucha_z_amisske_komunity_pomohla_potvrdit_klicovy_mechanismus_v_bunce_4_79e003984a.jpeg" alt="CEITEC: A Rare Genetic Disorder in the Amish Community Helped Confirm a Key Cellular Mechanism: Pavla Gajdušková" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Vzacna_geneticka_porucha_z_amisske_komunity_pomohla_potvrdit_klicovy_mechanismus_v_bunce_4_79e003984a.jpeg 235w," sizes="100vw" width="1600" height="1067">This is where the research connects to patients in the Amish community. In affected children, a small genetic change in the SNIP1 protein disrupts its ability to respond properly to the signal mediated by the modified SF3B1 protein, making RNA splicing less efficient than in healthy individuals. As a result, errors occur during RNA splicing that the cell cannot correct, impairing its function – especially in the nervous system.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Vzacna_geneticka_porucha_z_amisske_komunity_pomohla_potvrdit_klicovy_mechanismus_v_bunce_44a7e2ef17.jpg" alt="CEITEC/Blažek Lab: A Rare Genetic Disorder in the Amish Community Helped Confirm a Key Cellular Mechanism: Interaction between SF3B1 and SNIP1 proteins." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Vzacna_geneticka_porucha_z_amisske_komunity_pomohla_potvrdit_klicovy_mechanismus_v_bunce_44a7e2ef17.jpg 156w," sizes="100vw" width="1600" height="1600"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Vzacna_geneticka_porucha_z_amisske_komunity_pomohla_potvrdit_klicovy_mechanismus_v_bunce_2_85826d711e.jpg" alt="CEITEC/Blažek Lab: A Rare Genetic Disorder in the Amish Community Helped Confirm a Key Cellular Mechanism: The effect of mutations in SNIP1 on cell proliferation." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Vzacna_geneticka_porucha_z_amisske_komunity_pomohla_potvrdit_klicovy_mechanismus_v_bunce_2_85826d711e.jpg 235w," sizes="100vw" width="1600" height="1067">The researchers were thus able to clarify the cause of this rare neurodevelopmental disorder. “Additionally, the cause of this disease also confirms the correctness of the checkpoint we described. It shows that even a small change in one of the proteins involved can disrupt the RNA splicing mechanism on which the cell depends,” adds group leader <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.eu/dalibor-blazek-ph-d/u25918">Dalibor Blažek</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Vzacna_geneticka_porucha_z_amisske_komunity_pomohla_potvrdit_klicovy_mechanismus_v_bunce_3_f95cfd68eb.jpeg" alt="CEITEC/Martin Indruch: A Rare Genetic Disorder in the Amish Community Helped Confirm a Key Cellular Mechanism: Dalibor Blažek." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Vzacna_geneticka_porucha_z_amisske_komunity_pomohla_potvrdit_klicovy_mechanismus_v_bunce_3_f95cfd68eb.jpeg 235w," sizes="100vw" width="1600" height="1067">Although the study focuses on basic research, its implications are broader. It suggests that the same mechanism may also play a role in regulating cell division – a process that is often disrupted in cancer.<blockquote>The research was carried out by scientists from CEITEC Masaryk University in collaboration with researchers from Spain and Germany. The study was published in the journal <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-71119-2">Nature Communications</a>.</blockquote>

A Rare Genetic Disorder in the Amish Community Helped Confirm a Key Cellular Mechanism

This study investigates the pathogenesis of neurosyphilis through cerebrospinal fluid proteomics using LC–MS/MS and ELISA validation. Comparative analysis identified 234 differentially expressed proteins, with major alterations linked to lysosomal dysfunction, axon guidance, and neurodegeneration pathways.Key lysosomal proteins, including PSAP and DNASE2, together with the axonal protein EPHA4, were significantly downregulated in neurosyphilis patients. These proteins showed potential as biomarkers for neurodegeneration and may provide new insights into disease mechanisms, diagnostics, and therapeutic targets.<blockquote><h4>The original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689">Cerebrospinal Fluid Proteome Reveals Dysregulation of Lysosomal and Axonal Proteins in Neurosyphilis</a></h4>Huayang Tang*, Junjie Hu, Yifan Wu, Jinping Gao, Wenjun Wang, Xiaodong Zheng, Ran Zhang, Bo Liang, Fusheng Zhou, Ze Guo*J. Proteome Res. 2026, 25, 2, 713–722<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.jproteome.5c00689">https://doi.org/10.1021/acs.jproteome.5c00689</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.<div>Neurosyphilis (NS) is a group of clinical syndromes characterized by neuroinflammation caused by Treponema pallidum (T. pallidum), which occurs in all stages of syphilis. <a href="javascript:void(0);">(1,2)</a> NS is classified based on clinical presentation as either asymptomatic (ANS) or symptomatic (SNS), and based on disease stage as either early (occurring 1–2 years after primary infection) or late. <a href="javascript:void(0);">(1)</a> T. pallidum can cause local nonspecific and central nervous system (CNS) inflammation, leading to possible degenerative changes. <a href="javascript:void(0);">(3)</a> The immune response of CNS in neurosyphilis is comparable to that observed in other neuroinflammatory diseases. Consequently, the presence of neurological symptoms may not reliably indicate active CNS inflammation in syphilis patients. <a href="javascript:void(0);">(3)</a> A cerebrospinal fluid (CSF) proteomic study identified three biomarkers (SEMA7A, SERPINA3, and ITIH4) that contribute to NS and have potential implications for diagnosis and therapeutic strategies. <a href="javascript:void(0);">(4)</a> Through integrated proteomics and single-cell transcriptomics analyses, Toll-like/NF-κB signaling was identified as the key pathway involved in CNS damage caused by T. pallidum. <a href="javascript:void(0);">(5)</a> Despite an increasing understanding of the biomarkers and possible neuroinflammatory damage mechanisms of neurosyphilis, the mechanisms of neurodegeneration caused by T. pallidum infection remain elusive. <a href="javascript:void(0);">(6)</a>Patients with neurosyphilis exhibit stage-specific profiles of soluble factors in their CSF, which are linked to both immune status and the extent of neuronal damage. <a href="javascript:void(0);">(7)</a> In this study, we analyzed differentially expressed proteins (DEPs) in the CSF proteomes from 28 individuals, including neurosyphilis (NS), suspected neurosyphilis (spNS) and non-neurosyphilis (nNS) individuals, by LC–MS/MS and analyzed a public data set of the neurosyphilis CSF proteome. Validation was then performed by ELISA in an independent group comprising 110 samples. The results revealed that neuroinflammation by T. pallidum infection results in dysregulation of the key proteins in lysosomal and axonal pathway, which suggesting a potential contribution to neurodegeneration in neurosyphilis.<h4>Materials and Methods</h4><h5>LC–MS/MS</h5>The tryptic peptides were dissolved in 0.1% formic acid (solvent A) and loaded onto a custom-made reversed-phase column (15 cm × 75 μm). Using an EASY-nLC 1000 UPLC system, a gradient was run at 400 nL/min as follows: 6% to 23% solvent B (0.1% formic acid in 98% acetonitrile) over 26 min, 23% to 35% in 8 min, up to 80% in 3 min, and held at 80% for 3 min. Peptides were ionized via an NSI source at 2.0 kV and analyzed online by a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/559">Q Exactive Plus mass spectrometer</a>. Full scans (m/z 350–1800) were acquired in the Orbitrap at 70,000 resolution. The top precursors were selected for MS/MS with an NCE of 28, and fragments were detected at 17,500 resolution. A data-dependent method cycled between one MS scan and 20 MS/MS scans with a 15.0 s dynamic exclusion. AGC was set at 5E4. MS/MS data were processed with MaxQuant (v.1.5.2.8) using Trypsin/P as the enzyme (up to 2 missed cleavages). Precursor mass tolerance was 20 ppm (first search) and 5 ppm (main search); fragment mass tolerance was 0.02 Da. Fixed modification: carbamidomethyl (C); variable modification: oxidation (M). The FDR threshold was &lt;1%, and the minimum peptide score was &gt;40.<h5>ELISA</h5>CSF levels of host proteins were quantified using commercial ELISA kits according to the manufacturers’ protocols. Proteins analyzed included PSAP (OKEH07124, Aviva, USA), CTSL (abx150963, Abbexa, UK), NPC2 (A-QEK07857, Biogradetech, USA), and DNASE2 (abx386953, Abbexa, UK). Briefly, 100 μL of CSF was added to precoated plates and incubated at 37 °C for 2 h. After washing, 100 μL of detection antibody was added to each well and incubated at 37 °C for another 2 h. Following a wash, 100 μL of streptavidin-HRP working solution was added and incubated at room temperature for 20 min in the dark. The reaction was stopped with 50 μL of stop solution, and the optical density was measured at 450 nm.<h4>Results</h4><h5>Downregulation of Key Lysosomal Proteins in CSF from Patients With Neurosyphilis</h5>By analyzing the DEPs in NS vs nNS, we observed a significant enrichment of downregulated proteins in the lysosome pathway (P = 0.007) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig2">Figure 2</a>A), with 9 lysosomal proteins showing significant downregulation (Padj &lt; 0.05) in the NS group (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.5c00689/suppl_file/pr5c00689_si_001.zip">Table S4</a>). To validate this finding, we examined the public CSF proteomics data set of neurosyphilis (PXD033034) and identified 18 proteins in the lysosomal pathway that were significantly downregulated in patients with NS (Padj &lt; 0.05) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.5c00689/suppl_file/pr5c00689_si_001.zip">Table S4</a>). PPI network analysis determined the top 10 hub genes within the lysosome pathway (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig2">Figure 2</a>B). A Venn diagram revealed 3 proteins (PSAP, CTSL, and NPC2) shared across our current data, the public data set (PXD033034) and the top 10 hub genes (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig2">Figure 2</a>C). The mass spectrometry results indicated that the levels of PSAP (Padj = 0.043), CTSL (Padj = 0.028) and NPC2 (Padj = 0.013) were significantly lower in the NS group compared to the nNS group (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig2">Figure 2</a>D). The PXD033034 data set revealed consistent changes in the PSAP, CTSL, and NPC2 levels in the NS group (Padj &lt; 0.05) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.5c00689/suppl_file/pr5c00689_si_001.zip">Figure S1 and Table S4</a>). In addition, associations between the lysosomal protein DNASE2 and NS were observed in both the present study (Padj = 0.003) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig2">Figure 2</a>D) and the PXD033034 data set (Padj &lt; 0.05) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.5c00689/suppl_file/pr5c00689_si_001.zip">Figure S1 and Table S4</a>), with subgroup analysis revealing a significantly lower expression level of DNASE2 in the SNS group compared to the ANS group (P = 0.035) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig2">Figure 2</a>D and <a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.5c00689/suppl_file/pr5c00689_si_001.zip">Table S3</a>). Further validation by ELISA (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.5c00689/suppl_file/pr5c00689_si_001.zip">Table S5</a>) in an independent group (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#tbl1">Table 1</a>) confirmed significantly downregulated CSF levels of PSAP (P &lt; 0.0001), CTSL (P &lt; 0.0001), NPC2 (P = 0.0024) and DNASE2 (P &lt; 0.0001) in patients with NS (n = 21) than in nNS individuals (n = 56) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig2">Figure 2</a>E) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.5c00689/suppl_file/pr5c00689_si_001.zip">Table S6</a>), as well as decreased in the SNS subgroup (P &lt; 0.05) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.5c00689/suppl_file/pr5c00689_si_001.zip">Figure S2 and Table S6</a>). Receiver operating characteristic (ROC) curve analysis supported PSAP (AUC = 0.91, 95% CI: 0.83–0.99) and DNASE2 (AUC = 0.95, 95% CI: 0.91–0.99) for distinguishing NS from nNS and as potential biomarkers for assessing neurodegeneration in NS (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig2">Figure 2</a>F). These results indicate that T. pallidum infection may lead to lysosomal dysfunction in CNS.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/J_Proteome_Res_2026_25_2_713_722_Figure_2_Downregulation_of_key_lysosomal_proteins_in_the_cerebrospinal_fluid_of_neurosyphilis_patients_bbccd95f44.jpg" alt="J. Proteome Res. 2026, 25, 2, 713–722: Figure 2. Downregulation of key lysosomal proteins in the cerebrospinal fluid of neurosyphilis patients. (a) KEGG pathway analysis demonstrated that downregulated proteins in patients with NS were significantly enriched in the lysosome pathway, as well as in axon guidance and neurodegeneration. (b) The top 10 hub genes involved in the lysosome pathway were identified using the STRING network data set and PPI analysis. (c) A Venn diagram revealed 3 proteins (PSAP, CTSL, and NPC2) shared across our current data, the public data set (PXD033034) and the top 10 hub genes. (d) The CSF levels of PSAP (Padj = 0.043), CTSL (Padj = 0.028), DNASE2 (Padj = 0.003) and NPC2 (Padj = 0.013) were significantly decreased in the NS group than in the nNS group. Subgroup analysis found a significantly lower expression level of DNASE2 in the SNS group than in the ANS group (P = 0.035). (e) PSAP, DNASE2, CTSL and NPC2 levels in CSF were tested by ELISA in the nNS (n = 56) and NS (n = 21) groups. Compared with those in the nNS group, significantly lower PSAP (P &lt; 0.0001), CTSL (P &lt; 0.0001), DNASE2 (P &lt; 0.0001) and NPC2 (P = 0.0024) levels were detected in the NS group. (f) ROC curve analysis for NS revealed that PSAP had an AUC = 0.91 (95% CI: 0.83–0.99), DNASE2 had an AUC = 0.95 (95% CI: 0.91–0.99), CTSL had an AUC = 0.85 (95% CI: 0.76–0.95), and NPC2 had an AUC = 0.72 (95% CI: 0.59–0.85). NS: neurosyphilis; nNS: non-neurosyphilis; ANS: asymptomatic neurosyphilis; SNS: symptomatic neurosyphilis. ROC: receiver operating characteristic; AUC: area under the curve. * P &lt; 0.05, ** P &lt; 0.01, ****P &lt; 0.0001." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_J_Proteome_Res_2026_25_2_713_722_Figure_2_Downregulation_of_key_lysosomal_proteins_in_the_cerebrospinal_fluid_of_neurosyphilis_patients_bbccd95f44.jpg 140w," sizes="100vw" width="2100" height="2339"><h5>Downregulation of Key Axonal Proteins Correlated with Lysosomal Protein Reduction in Neurosyphilis CSF</h5>Lysosomes play crucial roles in neuronal health by maintaining axonostasis. <a href="javascript:void(0);">(10)</a> The enrichment analysis indicated that the downregulated proteins in NS were implicated in the biological processes of axonostasis (such as axon development and axonogenesis) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig1">Figure 1</a>F) and the pathway of axon guidance (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig2">Figure 2</a>A). Our proteomic data revealed a total of 24 downregulated (Padj &lt; 0.05) axonal proteins and 1 upregulated (Padj &lt; 0.05) axonal protein in the CSF of patients with NS (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.5c00689/suppl_file/pr5c00689_si_001.zip">Table S9</a>). Furthermore, the PXD033034 data set revealed 55 downregulated (Padj &lt; 0.05) and 4 upregulated (Padj &lt; 0.05) axonal proteins in NS (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/suppl/10.1021/acs.jproteome.5c00689/suppl_file/pr5c00689_si_001.zip">Table S9</a>). The top 10 hub genes involved in the biological processes of axon development and axonogenesis were identified by PPI network analysis (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig4">Figure 4</a>A). Considering the overlap of our current data, the PXD033034 data set and the top 10 hub genes (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig4">Figure 4</a>B), one key protein, EPHA4, was identified across 3 data sets, with significant downregulation in our current data (Padj = 0.011) and in the PXD033034 data set (Padj = 0.028) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig4">Figure 4</a>C). Correlation analysis further revealed a positive correlation between the levels of PSAP and EPHA4 (r = i 0.83, P &lt; 0.05) (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jproteome.5c00689#fig4">Figure 4</a>D). Reduced levels of PSAP and EPHA4 in CSF have been implicated in neurodegenerative diseases, including Alzheimer’s disease (AD) and Parkinson’s disease (PD). <a href="javascript:void(0);">(11,12)</a> Our findings suggest that coordinated dysregulation of lysosomal and axonal proteins may contribute to neurodegeneration in NS.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/J_Proteome_Res_2026_25_2_713_722_Figure_4_Key_axonal_protein_downregulated_in_CSF_from_patients_with_neurosyphilis_66f5533e94.jpg" alt="J. Proteome Res. 2026, 25, 2, 713–722: Figure 4. Key axonal protein downregulated in CSF from patients with neurosyphilis. (a) The top 10 hub genes involved in axonostasis were identified by the STRING network data set and PPI analysis. (b) A Venn diagram revealed 1 protein (EPHA4) shared across DEPs related to axonostasis in our current data and in the public data set (PXD033034) and the top 10 hub genes. (c) EPHA4 was significantly downregulated both in our present study (Padj = 0.011) and in the PXD033034 data set (Padj = 0.028). (d) Correlation analysis demonstrated that the EPHA4 level was significantly positively correlated (P &lt; 0.05) with the levels of PSAP (r = 0.83), CTSL (r = 0.6), NPC2 (r = 0.69), DNASE2 (r = 0.61) and SEMA7A (r = 0.83). NS: neurosyphilis; nNS: non-neurosyphilis. * P &lt; 0.05." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_J_Proteome_Res_2026_25_2_713_722_Figure_4_Key_axonal_protein_downregulated_in_CSF_from_patients_with_neurosyphilis_66f5533e94.jpg 178w," sizes="100vw" width="1250" height="1096"><h4>Conclusions</h4>The present study reveals the associations of dysregulated proteins in lysosomal and axonal pathways with neurosyphilis, and potential evidence of shared pathophysiological mechanisms between neuroinflammation caused by T. pallidum infection and neurodegenerative diseases.However, this study has certain limitations. First, the use of ELISA to validate a limited number of proteins has certain limitations, such as the inherent cross-reactivity of ELISA. In the future, we can optimize the validation experiments by adding techniques such as Western blotting for verification. Second, this study uses a cross-sectional sampling method for research, which limits our ability to infer disease progression and treatment responses. In the future, we will conduct follow-up on the subjects, continue collecting cerebrospinal fluid samples after treatment, and observe whether the protein dysregulation is reversible after treatment. For spNS patients, follow-up should also be conducted to determine if they have transformed into NS, which will be very valuable for validating the predictive utility of our biomarkers. Finally, although multiple discovery and validation cohort studies have been conducted, we currently lack direct mechanistic evidence. Future work will investigate whether exposing neural cell coculture models to outer-membrane antigens of T. pallidum reproduces the lysosome and axon alterations observed in CSF, clarifying their role in neural damage of neurosyphilis and potential biomarkers for diagnosis and treatment of neurodegeneration in neurosyphilis.</div>

Cerebrospinal Fluid Proteome Reveals Dysregulation of Lysosomal and Axonal Proteins in Neurosyphilis

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Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

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Waters XBridge BEH Phenyl HPLC Columns

Waters XBridge BEH Phenyl columns provide complementary selectivity to C18 stationary phases, especially for polyaromatic compounds.

XBridge BEH Phenyl columns provide complementary selectivity to C18 stationary phases, especially for polyaromatic compounds. This most chemically stable commercially available Phenyl sorbent on the market and provides you with ultra-low column bleed, long column lifetimes and excellent peak shape.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_Protein_C4_HPLC_Columns_0aa4f56918.jpeg" alt="Waters: XBridge BEH Phenyl HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_Protein_C4_HPLC_Columns_0aa4f56918.jpeg 234w," sizes="100vw" width="660" height="440"><h4>Specifications</h4><ul><li>Chemistry: Phenyl</li><li>Separation Mode: Reversed Phase</li><li>Particle Substrate: Hybrid</li><li>pH Range Min: 1 pH</li><li>pH Range Max: 12 pH</li><li>Maximum Pressure: 6000 psi (415 Bar)</li><li>Endcapped: Yes</li><li>Bonding Technology: Phenyl-Hexyl</li><li>Silanol Activity: Low</li><li>Particle Shape: Spherical</li><li>System: HPLC</li><li>Particle Technology: BEH</li><li>USP Classification: L11</li><li>Carbon Load: 15 %</li></ul><h4>The Waters XBridge BEH Phenyl HPLC Column is also available in these types:</h4><h5>Waters XBridge BEH Phenyl Column with eConnect Column Tag</h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: XBridge BEH Phenyl Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"><h5>Waters XBridge BEH Phenyl XP Column</h5><ul><li>Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.&nbsp;</li><li>Maximum Pressure: 10000 psi (690 Bar)</li><li>System: UHPLC, UPLC</li></ul><h3>XBridge Columns</h3><h4>Particle stability and performance for methods development</h4>Withstanding extreme operating conditions like high pH and high temperatures is essential for methods development. XBridge HPLC and UHPLC Columns utilize Ethylene Bridged Hybrid (BEH) Particle Technology and are built to withstand high pH, high temperatures, and other extreme operating conditions like volatile eluents, for a wide range of small molecule and biomolecule LC-MS analysis.XBridge HPLC and UHPLC Columns are available in a broad range of sorbent particles and sizes: XBridge eXtended Perfomance (XP) 2.5 µm Columns for rapid analytical UHPLC separations; 3.5 and 5 µm HPLC columns for reliable analytical method development; and 5 and 10 µm optimum bed density (OBD) preparative columns for purification chromatography.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_Columns_b0b55de501/Waters-XBridge-Columns.jpg" alt="Waters: XBridge Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_Columns_b0b55de501/thumbnail_Waters-XBridge-Columns.jpg 203w," sizes="100vw" width="900" height="691">

Thermo Scientific™ BioPharma Finder™ Software for the Characterization of Biotherapeutics

Simplify comprehensive biotherapeutic attribute characterization with advanced capabilities to increase throughput and confidence in a connected environment.

<div><div container-id="cq-colctrl-lt7" comp-id="A1"><div>Discover how the features of Thermo Scientific BioPharma Finder Software are engineered to support the world of complex biopharmaceutical characterization. Featuring novel data processing algorithms and easy-to-interpret data visualization tools, the software supports users looking to confidently characterize biopharmaceuticals.Power BioPharma Finder&nbsp;Software with the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1821"> Thermo Scientific Ardia Platform</a> to automate file centralization during acquisition, expand file access, enable report creation and result sharing, and remove data management barriers from your daily routine.<div><h3>Key benefits</h3></div><div><ul><li>Confident characterization: Explore a solution for confident animo acid sequence characterization with the Peptide Mapping Analysis module featuring a novel MS2 prediction algorithm.</li><li>Customized Nucleotide sequence support: Leverage the ability to create customized nucleotide sequences to support confirmation with the Oligonucleotide Analysis module featuring an improved MS2 fragmentation model (available in BioPharma Finder Software version 5.3).</li><li>Efficient data handling: Move through vast amounts of data with remarkable efficiency using the Intact Mass Analysis module. With innovative deconvolution algorithms and features, you can generate multi-consensus reports effortlessly, providing a comprehensive view of your results.</li><li>Unparalleled Modification Exploration and Flexibility: The Top-Down Analysis module empowers you to search through an extensive range of modifications, even at the per-peak level. With multiple fragmentation modes and a cross-array processing algorithm, you have the flexibility to explore your samples in greater detail.</li><li>Seamless integration: See how easy it is to move from sophisticated characterization using BioPharma Finder Software to confident quantitation in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS software</a> with benefits added by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1821">Ardia Platform</a> including connecting instrument data, supporting global access and centralizing BioPharma Finder reports.</li></ul><h3>Learn more about BioPharma Finder Software</h3><div>The right combination of data and software makes all the difference in accurately characterizing molecules, especially for large molecule.<h4>Peptide mapping analysis</h4>Characterization of a macromolecule comprised of an amino acid sequence can involve a lot of moving targets. Techniques for confident confirmation typically include comparisons of data collected against theoretical knowledge of the sequence. The best processing tools will also give users the ability to model probable sequences against the data collected with easy-to-use tools to increase the confidence of the identification and supply the summary reports with easy-to-interpret results.<div><h5>Featured in the Peptide Mapping Analysis</h5><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_b88e243207.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: Processing with the novel MS2 algorithm in the Peptide Mapping Analysis module, generates a predictive model that includes Kinetical predictions that are compared with the measured spectra to increase confidence in the identification." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_b88e243207.jpg 245w," sizes="100vw" width="1160" height="540"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_2_6dda72b394.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: These processing tools, when combined, produce intuitive result review tools including comprehensive coverage maps, deep dive spectral comparison views and graphical sample comparison options." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_2_6dda72b394.jpg 245w," sizes="100vw" width="1160" height="600"><div><h5>Detect and quantify host cell proteins</h5></div><div>The sensitivity of the detection algorithm and prediction analytics allow for high confidence even for low abundance targets to support residuals applications like Host Cell Protein (HCP) analysis.</div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_3_e493898dc1.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: Detect and quantify host cell proteins." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_3_e493898dc1.jpg 245w," sizes="100vw" width="1160" height="600"></div><h4>Oligonucleotide analysis</h4><div>Sequence confirmation for oligonucleotides can be daunting. Take the question out of sequence confirmation with our enhanced MS2 predictive algorithms that can iteratively create the best array of possibilities without iterating into infinity. Summarize your characterization with visual tools, generate easy-to-interpret results and enhance collaboration by saving and sharing reports in a connected environment.&nbsp;&nbsp;<h5>Featured in the Oligonucleotide Analysis module</h5><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_4_f709ce5528.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: The oligonucleotide sequence editor provides an easy-to-use interface for quickly and easily building and visualizing customized oligonucleotide sequences. Build out multiple expected sequences from custom building block subunits, a wide selection of modifications from the predefined list or with user-defined custom modifications." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_4_f709ce5528.jpg 245w," sizes="100vw" width="1160" height="600"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_5_f204e2cd40.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: Eliminate alternative possibilities based on the entered sequence with the random sequence generator and leverage the in silico digest tools to support confident sequence verification - all using an updated MS2 prediction algorithm (available in BioPharma Finder Software version 5.3)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_5_f204e2cd40.jpg 245w," sizes="100vw" width="1160" height="600"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_6_f7f2d9e029.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: Perform mRNA mapping with selected in silico mRNA digestion or define the cleavage locations manually. Dig into the identity of fragments and expected or unexpected modifications and assess the overall coverage with intuitive visual coverage maps." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_6_f7f2d9e029.jpg 245w," sizes="100vw" width="1160" height="600"></div><h4>Intact mass analysis</h4><div><div container-id="cq-colctrl-lt2" comp-id="B1"><div>Intact analysis on its face is a simple mathematical calculation used to deconvolute a series of m/z values into components but throw in the complexity of large molecule targets with modifications in a mixture of other like-molecules and mass accuracy becomes harder to achieve. No matter your data resolution, leverage innovative software tools engineered to filter and process through complex spectra with the Intact Analysis module. Discover how to quickly acquire and process batches of samples supported by a central data location on the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1821">Ardia Platform</a> and speed through large batch-to-batch comparison studies to create summaries with connected multi-consensus reports.<div><h5>Featured in the Intact Analysis module</h5><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_7_72cfbd44c3.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: Accurately determine molecular weights utilizing the Sliding Window algorithm coupled with our optimized Xtract (for high resolution) and Respect (for lower resolution) deconvolution algorithms. Achieve comprehensive detection, even for co-eluting, low-intensity components in complex mixtures and account for any shifts in retention time." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_7_72cfbd44c3.jpg 245w," sizes="100vw" width="1160" height="323"></div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_8_d0c92fbca9.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: Use the expanded sequence matching capabilities for oligonucleotides which includes the ability to perform Terminal Truncation Searching from either the 3' or 5' terminus, facilitating the identification of impurities with greater ease. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_8_d0c92fbca9.jpg 245w," sizes="100vw" width="1160" height="600"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_9_d345484402.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: The intact mass analysis of heavily modified oligonucleotides presents analytical and data processing challenges due to the frequent usage of sulfur modifications. Pairing Thermo Scientific Orbitrap mass spectrometers with BioPharma Finder Software allows for confident intact mass identification of phosphorothioate conversion of oligonucleotides with less than 5 ppm error." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_9_d345484402.jpg 245w," sizes="100vw" width="1160" height="600"></div></div></div><h4>Top-down analysis</h4><div><div container-id="cq-colctrl-lt2" comp-id="B1"><div>Higher order structure characterization requires a combination of some of the most variable sample treatment techniques, variable data acquisition techniques, and combined or complex data processing techniques. Looking to gently pry apart large complexes without reducing them to their smallest parts and understand them is an art and a science that can be supported by the Top-Down Analysis module. Explore the tools offered to help refine the field of predicted possibilities without restricting them, the support for multi fragmentation mode data, and the easy-to-use multi-consensus summaries to understand your molecule.<div><h5>Featured in the Top-Down Analysis module</h5><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_10_7e7ef85413.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: With the ProSightBP processing tool users can set variable or static sites for modification, select peak specific deconvolution parameters and even select peak specific sequence searching then let ProSightBP create the predictions. Combine the data from multi fragmentation modes including CID, HCD, ETD, EThcD, and UVPD resulting in confident identification." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_10_7e7ef85413.jpg 245w," sizes="100vw" width="1160" height="600"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_11_e3c9f5084b.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: Combining the predictive power of ProSight BP against the deconvoluted accurate mass with the sequence coverage from ETD and UVPD fragmentation reveals a comprehensive set of results to support confident molecule characterization." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_11_e3c9f5084b.jpg 245w," sizes="100vw" width="1160" height="600"></div></div></div></div><h4>Connecting to the Ardia Platform</h4><div>Living in a world connected by networks that enable people to share everything doesn’t have to stop in the lab. With the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1821"> Ardia Platform</a> see how easy it can be to automatically centralize files, connect into those files from anywhere and process them with BioPharma Finder Software or any connected application.&nbsp;&nbsp;<div><h5>Featured enhancements through connection</h5><div><div container-id="cq-colctrl-lt2" comp-id="B1"><div>Faster data review<ul><li>Review a result generated by another colleague without having to import the results, open it from the shared secured Ardia Platform data repository, make comments or changes, and the software will automatically generate a new version to record the changes.</li></ul>Better data management with centralized secured data<ul><li>All results are securely saved in one place, no more finding the “right” computer or hard drive that contains the experiment and data.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_12_b6a814cd90.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: Keep data secure and controlled. Gain insights into file history, method history, and manage user access with control." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_12_b6a814cd90.jpg 245w," sizes="100vw" width="1160" height="600"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Bio_Pharma_Finder_Software_13_1e3b879f18.jpg" alt="Thermo Scientific™ BioPharma Finder™ Software: Save and review individual reports without having to open BioPharma Finder Software for oligonucleotide, peptide mapping and intact mass applications. Utilize default reporting templates to speed development of customized reports and effectively modify templates to achieve desired result summary with the help of the customizable report designer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Bio_Pharma_Finder_Software_13_1e3b879f18.jpg 245w," sizes="100vw" width="1160" height="600">Enable collaboration on a global scale in a secure fashion to collaborate, compare, track, and finalize peptide results and workbooks using dynamic versioning history. Accelerate method optimization and review through a shared secure environment.</div></div></div></div></div></div></div></div></div></div>

Thermo Scientific SII for Empower Software

Incorporate and operate Thermo Scientific UHPLC, HPLC, GC, and IC instruments within other vendors' software platforms with the Thermo Scientific™ SII for Empower 3 Software plug-in.

<div id="product-description">Incorporate and operate Thermo Scientific UHPLC, HPLC, GC, and IC instruments within other vendors' software platforms with the Thermo Scientific™ Standard Instrument Integration (SII) for Empower 3 Software plug-in. The SII for Empower 3 plug-in is based on the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)</a> and offers unique and smart features that deliver maximum ease-of-use, reliability, and performance. It also enables Thermo Scientific™ chromatography systems to be operated and controlled by <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627">Waters™ Empower™ 3 CDS software</a>.</div><div>While Chromeleon CDS is the recommended software for control of Thermo Scientific UHPLC, HPLC, GC, and IC instruments due to its maximum ease of use, reliability, and performance, other vendors' CDS software can also incorporate and operate these instruments with the SII for Empower™ 3 Software plug-in.The SII for Empower™ Software installs and uses portions of <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a>, such as the Services Manager, Instrument Configuration Manager, Instrument View, and Instrument Method Editor, to deliver flawless integration of Thermo Scientific LC, IC, and GC instrumentation into Empower™ 3 CDS without loss of functionality. Instrument control, data acquisition, and data processing are performed completely under Empower™ 3 Software.Configure your instruments Up to four instruments can be configured per Empower™ node using the standard Chromeleon Instrument Configuration Manager.Control your instruments SII for Empower™ Software provides a complete set of commands because they are present under native Chromeleon CDS control. For the user, this means that all the Chromeleon CDS tools and advanced features, such as preparing the next sample during an ongoing analysis, can be implemented to increase productivity and receive more data with fewer injections.SII for Empower™ controls the following systems:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=37&amp;manufacturerItems=3&amp;search=Vanquish">Thermo Scientific™ Vanquish™ HPLC and UHPLC systems</a></li><li>Thermo Scientific™ UltiMate™ 3000 HPLC systems</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/410">Thermo Scientific™ Dionex™ ICS-6000 HPIC™ system</a></li><li>Thermo Scientific™ Dionex™ ICS-5000 HPIC™ system</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/408">Thermo Scientific™ Dionex™ Integrion™ HPIC™ system</a></li><li>Thermo Scientific™ Dionex™ Aquion™ system</li><li>Thermo Scientific™ TRACE™ 1300 GC systems</li></ul>Monitoring instrument status within Empower™ can be performed using the Empower™ Control Panel. Here, an enhanced SII Instrument View is easily accessed to provide further options to control and monitor the instrument and its modules.Instrument Methods Setting up an instrument method is simple, intuitive, and fast due to the SII for Empower™ Instrument Method Wizard and Editor, both of which are integrated into the Instrument Method Editor of Empower™. The wizard guides the user through all modules, enabling the entry of all necessary information, while the editor allows the review and modification of all aspects of a method.Process your data Complete data processing takes place inside Empower™ Software, which allows you to use all functionalities as you would normally, for example, overlay of chromatograms or other channels like pressure traces. The Empower™ user experience does not change for any data processing aspect.</div>

Thermo Scientific LC-MS Data Acquisition Software

Acquire the highest quality data possible to unlock the information, knowledge, and value in your samples with Thermo Scientific LC-MS Data Acquisition Software.

Thermo Scientific™ Chromeleon™ XPS Open Access Software

Thermo Scientific™ Chromeleon™ XPS provides streamlined and easy-to-use interface for Thermo Scientific™ Chromeleon™ CDS, while maintaining full capabilities of the CDS software.

In modern laboratories, chromatography techniques and the use of mass spectrometry (MS) detection are in high demand. Until recently, MS detectors were regarded as sophisticated detection tools that could be operated only by a highly trained specialist. Nowadays mass spectrometers are increasingly used as straightforward detectors for chromatography.Alongside this move into routine use of MS is the continued deskilling of staff in the laboratory world, causing highly experienced chromatographers to be in short supply. The result is a substantial number of regular scientists and process operators that need to generate chromatographic results using advanced instrumentation themselves.The solution is an Open Access tool enabling users to simply walk up and use a very straightforward interface to exploit the full power of the chromatography data system (CDS), without actually using the CDS software itself. Thermo Scientific™ Chromeleon™ XPS provides this streamlined and easy-to-use interface for <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Thermo Scientific™ Chromeleon™ CDS</a>, while maintaining full capabilities of the CDS software, like instrument control, data processing, reporting and compliance tools.<h3>Workflow</h3>Users can walk up to any chromatography instrument connected to the CDS, with or without MS detection, and start to run their analysis with only a few mouse-clicks.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_5e8f8c3dec.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: First, select the analysis to perform (A) and which instrument should be used (B). The instrument overview shows the current instrument status’ and displays the estimated wait time until each instrument is available and the number of sample positions still available. The instrument list is filtered depending on the analysis selected and the users privileges to only show appropriate instruments." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_5e8f8c3dec.jpg 196w," sizes="100vw" width="876" height="697"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_2_a8b64a52ae.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: Next, enter the sample names (C) and any other parameters (D) required for the analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_2_a8b64a52ae.jpg 196w," sizes="100vw" width="876" height="697"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_3_4b4367ffae.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: Place the sample vials in the indicated positions and select Submit." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_3_4b4367ffae.jpg 196w," sizes="100vw" width="876" height="697"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_4_0386d31c9a.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: The sequence is submitted and will run. The operator is shown the expected start and finish time of the analyses." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_4_0386d31c9a.jpg 245w," sizes="100vw" width="625" height="263"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_5_1b51ab8dd2.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: At the end of the run, the results are automatically printed and/or exported and a link to the exported report can be distributed via email." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_5_1b51ab8dd2.jpg 245w," sizes="100vw" width="814" height="309"><h3>Configuration and management</h3>Running samples is as easy as described above once Chromeleon XPS software is set up, configured and managed by an experienced chromatographer.<ul><li>All the methods required for the analysis must be created; the instrument method with all instrument control and data acquisition parameters, the processing method with all data processing parameters and the report template with all required results, to be reported.&nbsp;</li><li>A Chromeleon eWorkflow is created using these methods. An eWorkflow is an electronic procedure that automates all steps of the laboratory process related to a chromatographic analysis</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_6_a3b2219e42.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: The lab manager can customize Chromeleon XPS to show or hide individual toolbar buttons (A), which eWorkflows are visible to users (B) and which instruments they can access (C)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_6_a3b2219e42.jpg 196w," sizes="100vw" width="876" height="697"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_7_d647111f5d.jpg" alt="Thermo Scientific™ Chromeleon™ XPS Open Access Software: In addition, it is possible to define various system options, such as which email notifications to send." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Scientific_Chromeleon_XPS_Open_Access_Software_7_d647111f5d.jpg 193w," sizes="100vw" width="659" height="533">Chromeleon XPS Open Access software lets any user run samples on their own by delivering a clean, streamlined walk-up interface that utilizes the full power of Chromeleon CDS. Users can access all instruments controlled by <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76">Chromeleon CDS</a>, including MS, and automatically receive results or notifications about run completion or failure. The management console contains advanced customization options to fulfill any organization’s requirements, included fully regulated environments.Chromeleon XPS software provides error-free execution of any chromatography analysis with minimal training requirements for users.

Waters XSelect HSS Cyano (CN) HPLC Columns

Waters XSelect HSS Cyano column is a low coverage, unendcapped, highly stable cyano phase that provides low hydrophobicity for the rapid elution of hydrophobic analytes

The XSelect HSS Cyano column is a low coverage, unendcapped, highly stable cyano phase that provides low hydrophobicity for the rapid elution of hydrophobic analytes as well as alternate selectivity to traditional alkyl chain columns. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_HPLC_Column_e96c520ca3.jpg" alt="Waters: XSelect HSS Cyano (CN) HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_HPLC_Column_e96c520ca3.jpg 234w," sizes="100vw" width="660" height="440"><h4>Specifications</h4><ul><li>Chemistry: Cyano (CN)</li><li>Separation Mode: Reversed Phase</li><li>Particle Substrate: Silica</li><li>pH Range Min: 2 pH</li><li>pH Range Max: 8 pH</li><li>Maximum Pressure: 10000 psi (690 Bar)</li><li>Endcapped: Yes</li><li>Silanol Activity: High</li><li>Particle Shape: Spherical</li><li>Pore Size: 100 Å</li><li>System: UHPLC, UPLC</li><li>Particle Technology: HSS</li><li>USP Classification: L10</li><li>Carbon Load: 5 %</li></ul><h3>XSelect Columns</h3><h4>Achieve unmatched reproducibility with a wide range of selectivity</h4>Quickly and easily develop robust methods compatible with all modern chromatographic detection modes and transfer them from lab to lab with the XSelect Column family.Designed to help you achieve reproducible and highly selective chromatographic separations, the XSelect Column family features two base particles technologies, Charged Surface Hybrid (CSH) and High Strength Silica (HSS). With XSelect Columns, method development scientists like you can achieve a wide range of selectivities across all application areas, without compromising performance.<h4>Overview</h4><ul><li>Designed for selectivity and stability at low pH</li><li>Superior peak shape for basic compounds</li><li>Highest analyte mass loading available</li><li>Excellent batch-to-batch reproducibility</li><li>Ideal for rapid method development</li><li>CSH Column chemistries: C18, Fluoro-Phenyl (PFP), and Phenyl-Hexyl, available in 2.5 XP, 3.5, 5 μm analytical and 10 μm OBD preparative sizes</li><li>HSS Column chemistries: HSS C18, C18 SB, and T3, available in 2.5 XP, 3.5, and 5 μm particle sizes</li><li>Prolonged column lifetimes with optional VanGuard Cartridges specifically designed using XSelect stationary phases</li><li>Recommended Use: For improving selectivity, peak shape, and retentivity in methods development.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Select_Columns_ff2c4ddb9e/Waters-XSelect-Columns.jpg" alt="Waters: XSelect Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Select_Columns_ff2c4ddb9e/thumbnail_Waters-XSelect-Columns.jpg 242w," sizes="100vw" width="900" height="580">

Waters Atlantis dC18 HPLC Columns

Waters Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. 

Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. The Atlantis dC18 columns feature di-functionally bonded C18 ligands that have been optimized for use with highly aqueous mobile phases, including 100% water.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Atlantis dC18 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"><h4>Specifications</h4><ul><li>Chemistry: C18</li><li>Separation Mode: Reversed Phase</li><li>Particle Substrate: Silica</li><li>pH Range Min: 3 pH</li><li>pH Range Max: 7 pH</li><li>Maximum Pressure: 6000 psi (415 Bar)</li><li>Endcapped: Yes</li><li>Bonding Technology: dC18</li><li>Silanol Activity: Medium</li><li>Particle Shape: Spherical</li><li>Pore Size: 100 Å</li><li>System: HPLC</li><li>USP Classification: L1</li><li>Carbon Load: 12 %</li></ul><h4>The Waters Atlantis dC18 HPLC Column is also available in these types:</h4><h5>Waters Atlantis dC18 HPLC Column&nbsp;with eConnect Column Tag</h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: Atlantis dC18 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"><h5>Waters Atlantis dC18 Intelligent Speed (IS) Column</h5><ul><li>Experience exceptional liquid chromatography performance with the Atlantis dC18 Intelligent Speed (IS) Column—a cutting-edge, high-resolution solution designed for the efficient separation of a wide range of polar and non-polar compounds, catering to diverse applications such as peptide analysis, protein analysis, small molecule analysis, and drug discovery.</li><li>What sets the Atlantis dC18 Intelligent Speed (IS) Column apart is its innovative Intelligent Speed (IS) technology. This proprietary technology enables faster analysis times without compromising separation efficiency, making it an ideal choice for laboratories that require rapid analysis of large sample volumes and reliable lab equipment.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg" alt="Waters: Atlantis dC18 Intelligent Speed (IS) Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg 234w," sizes="100vw" width="660" height="440"><h3>Atlantis Columns</h3><h4>Reversed-phase and HILIC HPLC Columns for superior polar compound retention</h4>Polar analytes are difficult to retain using traditional reversed-phase (RP) chromatography as they often elute in the void volume of the column. You can solve this challenge today with Atlantis Columns, which retain and separate polar analytes under reversed phase and HILIC conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.Available in T3, dC18, and the unbonded HILIC chemistries, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures.&nbsp;<h4>Overview</h4><ul><li>Retains and separates small, water-soluble polar organic compounds</li><li>Compatible with 100% aqueous mobile phases</li><li>Improves detection limits, method reproducibility, and robustness by eliminating ion-pairing reagents</li><li>Long column lifetimes using low-pH mobile phases</li><li>Fully scalable in 3, 5, and 10 μm particle sizes</li><li>Recommended Use: For the reproducible retention and separation of a broad range of polar and non-polar compounds.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_HPLC_Columns_6651612161.jpg" alt="Waters: Atlantis HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_HPLC_Columns_6651612161.jpg 234w," sizes="100vw" width="1024" height="682">

Waters Atlantis T3 HPLC Columns

Waters Atlantis T3 columns are universal, silica-based, reversed-phase C18 columns that provide balanced retention of polar and hydrophobic molecules

Atlantis T3 columns are universal, silica-based, reversed-phase C18 columns that provide balanced retention of polar and hydrophobic molecules and should be your first choice when developing separations of polar and non-polar compounds.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Atlantis T3 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"><h4>Specifications</h4><ul><li>Chemistry: C18</li><li>Separation Mode: Reversed Phase</li><li>Particle Substrate: Silica</li><li>pH Range Min: 2 pH</li><li>pH Range Max: 8 pH</li><li>Maximum Pressure: 6000 psi (415 Bar)</li><li>Endcapped: Yes</li><li>Bonding Technology: T3</li><li>Silanol Activity: Medium</li><li>Particle Shape: Spherical</li><li>Pore Size: 100 Å</li><li>System: HPLC</li><li>USP Classification: L1</li><li>Carbon Load: 14 %</li></ul><h4>The Waters Atlantis T3 HPLC Column is also available in these types:</h4><h5>Waters Atlantis T3 HPLC Column&nbsp;with eConnect Column Tag</h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: Atlantis T3 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"><h5>Waters Atlantis T3 Intelligent Speed (IS) Column</h5><ul><li>Your first option when creating separations of polar and non-polar compounds should be Atlantis T3 columns, which are universal, silica-based, reversed-phase C18 columns that allow balanced retention of polar and hydrophobic molecules. A trifunctional C18 alkyl phase is used in the Atlantis T3 Column's innovative T3 bonding technique, which is bonded at a ligand density that encourages polar compound retention and compatibility with water mobile phases. When compared to the conventional trimethyl silane (TMS) end-capping, the patented T3 end-capping method is significantly more efficient. You get better polar compound retention and aqueous compatibility when the special end-capping and T3 bonding are combined.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg" alt="Waters: Atlantis T3 Intelligent Speed (IS) Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg 234w," sizes="100vw" width="660" height="440"><h3>Atlantis Columns</h3><h4>Reversed-phase and HILIC HPLC Columns for superior polar compound retention</h4>Polar analytes are difficult to retain using traditional reversed-phase (RP) chromatography as they often elute in the void volume of the column. You can solve this challenge today with Atlantis Columns, which retain and separate polar analytes under reversed phase and HILIC conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.Available in T3, dC18, and the unbonded HILIC chemistries, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures.&nbsp;<h4>Overview</h4><ul><li>Retains and separates small, water-soluble polar organic compounds</li><li>Compatible with 100% aqueous mobile phases</li><li>Improves detection limits, method reproducibility, and robustness by eliminating ion-pairing reagents</li><li>Long column lifetimes using low-pH mobile phases</li><li>Fully scalable in 3, 5, and 10 μm particle sizes</li><li>Recommended Use: For the reproducible retention and separation of a broad range of polar and non-polar compounds.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_HPLC_Columns_6651612161.jpg" alt="Waters: Atlantis HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_HPLC_Columns_6651612161.jpg 234w," sizes="100vw" width="1024" height="682">

Waters Atlantis Silica HILIC HPLC Columns

Waters Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% ACN) to provide retention of analytes that are too polar 

Atlantis HILIC Silica columns are used in combination with high organic mobile phases (&gt;80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.Due to their hydrophilic nature combined with their net positive charge at acidic pH, retention and separation of very basic polar analytes are extremely difficult using reversed-phase chromatography. The HILIC technology is able to resolve these challenging separation problems. This is what makes the Atlantis Silica HILIC Column the ideal lab equipment for use in metabolism, drug discovery, and combinatorial chemistries.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Atlantis Silica HILIC HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"><h4>Specifications</h4><ul><li>Chemistry: HILIC</li><li>Separation Mode: Hydrophilic Interaction (HILIC)</li><li>Particle Substrate: Silica</li><li>pH Range Min: 1 pH</li><li>pH Range Max: 5 pH</li><li>Maximum Pressure: 6000 psi (415 Bar)</li><li>Endcapped: No</li><li>Silanol Activity: High</li><li>Particle Shape: Spherical</li><li>Pore Size: 100 Å</li><li>System: HPLC</li><li>USP Classification: L3</li></ul><h4>The Waters Atlantis Silica HILIC HPLC Column is also available as:</h4><h5>Waters Atlantis Silica HILIC HPLC Column&nbsp;with eConnect Column Tag</h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: Atlantis Silica HILIC HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"><h3>Atlantis Columns</h3><h4>Reversed-phase and HILIC HPLC Columns for superior polar compound retention</h4>Polar analytes are difficult to retain using traditional reversed-phase (RP) chromatography as they often elute in the void volume of the column. You can solve this challenge today with Atlantis Columns, which retain and separate polar analytes under reversed phase and HILIC conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.Available in T3, dC18, and the unbonded HILIC chemistries, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures.&nbsp;<h4>Overview</h4><ul><li>Retains and separates small, water-soluble polar organic compounds</li><li>Compatible with 100% aqueous mobile phases</li><li>Improves detection limits, method reproducibility, and robustness by eliminating ion-pairing reagents</li><li>Long column lifetimes using low-pH mobile phases</li><li>Fully scalable in 3, 5, and 10 μm particle sizes</li><li>Recommended Use: For the reproducible retention and separation of a broad range of polar and non-polar compounds.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_HPLC_Columns_6651612161.jpg" alt="Waters: Atlantis HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_HPLC_Columns_6651612161.jpg 234w," sizes="100vw" width="1024" height="682">

Waters SunFire C18 HPLC Columns

Waters SunFire C18 columns are used for general purpose method development at low pH.

SunFire C18 columns are used for general purpose method development at low pH. The C18 ligand is highly retentive, especially for basic compounds and it is ideally suited for purification and impurity profile assays.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: SunFire C18 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"><h4>Specifications</h4><ul><li>Chemistry: C18</li><li>Separation Mode: Reversed Phase</li><li>Particle Substrate: Silica</li><li>pH Range Min: 2 pH</li><li>pH Range Max: 8 pH</li><li>Maximum Pressure: 6000 psi (415 Bar)</li><li>Endcapped: Yes</li><li>Silanol Activity: Low</li><li>Particle Shape: Spherical</li><li>Pore Size: 100 Å</li><li>System: HPLC</li><li>USP Classification: L1</li><li>Carbon Load: 16 %</li></ul><h4>The Waters SunFire C18 HPLC Column is also available in these types:</h4><h5>Waters SunFire C18 HPLC Column&nbsp;with eConnect Column Tag</h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS System running Empower software.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: SunFire C18 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"><h5>Waters SunFire C18 Intelligent Speed (IS) Column</h5><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg" alt="Waters: SunFire C18 Intelligent Speed (IS) Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_Intelligent_Speed_IS_Column_488e1ab406.jpg 234w," sizes="100vw" width="660" height="440"><h3>SunFire Columns</h3><h4>Superior mass loading at low pH</h4>High efficiency at a low pH can be challenging. Waters SunFire HPLC Columns, available in analytical and preparative dimensions, help you do just that with state-of-the-art bonded silica designed for high mass loading, excellent low pH stability, superior peak shapes, and high efficiency.SunFire Analytical Columns are available in 2.5 µm to 10 µm particle sizes across two reversed-phase column chemistries, C18 and C8, sure to meet your silica-based HPLC column requirements. For preparative applications, in addition to the C18 and C8 chemistries, SunFire silica is offered in 5 and 10 µm particle sizes for preparative normal-phase purification.<h4>Overview</h4><ul><li>Exceptional loading capacity for both analytical and preparative columns</li><li>Excellent low pH stability for longer lifetimes</li><li>Superior peak shapes for faster and easier integration</li><li>Low mass spectral (MS) bleed, for enhanced analyte MS signal</li><li>Seamless scalability from analytical to optimum bed density (OBD) preparative columns</li><li>Recommended Use: For increased mass loading with excellent column stability.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Sun_Fire_Columns_3f48bd770c/Waters-SunFire-Columns.jpg" alt="Waters: SunFire Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Sun_Fire_Columns_3f48bd770c/thumbnail_Waters-SunFire-Columns.jpg 242w," sizes="100vw" width="900" height="580">

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