Microplastics and Nanomaterials: Understanding Emerging Contaminants in Environmental Testing

🎤Matthew BolinoHow do the hidden carbohydrate structures on your favorite protein powders shape the gut microbiome? &nbsp;In this episode of Concentrating on Chromatography, Matthew Bolino, M.S., from the University of Nevada, Reno, breaks down his latest research on N‑glycans from common dietary proteins (whey, egg white, soy, and pea) and how their structural diversity influences microbial fermentation and short‑chain fatty acid production.Bolino explains what N‑glycans are, why they behave like fiber in the gut, and how his team isolates and characterizes them using ethanol washes, enzymatic release (PNGase F and gut‑derived endoglycosidases), and advanced MALDI‑TOF and HILIC‑QTOF workflows. &nbsp;He also discusses his 2025 work comparing synthetic versus bovine whey N‑glycomes and mapping N‑glycan profiles across dietary protein sources, revealing how glycan architecture can reshape community diversity in in vitro fecal fermentations.Geared toward undergraduate and early‑career analytical chemists, this conversation dives into practical mass spec trade‑offs (MALDI vs QTOF vs LC/GC), real‑world troubleshooting in glycomics labs, and how microbiome‑targeted therapeutics and “symbiotic” designs may emerge from pairing specific microbes with preferred glycan structures. &nbsp;Bolino closes with career advice on building biomolecular analysis skills, understanding instrumentation fundamentals, and entering the rapidly growing field of glycomics and microbiome research.<h3>Video Transcription</h3><h4>Introduction</h4>Glycomics has emerged as a rapidly developing field within biomolecular science, expanding our understanding of post-translational modifications beyond well-known processes such as phosphorylation. Among these modifications, protein glycosylation—the covalent attachment of carbohydrate structures to proteins—plays a critical role in biological function, molecular recognition, and cellular communication.This discussion focuses on N-glycans, a class of structurally complex carbohydrates linked to proteins, and their occurrence in common dietary protein sources such as whey, egg white, soy, and pea protein. Particular emphasis is placed on their structural diversity, analytical workflows, and emerging relevance in gut microbiome research.<h4>Fundamentals of N-Glycans</h4>N-glycans are oligosaccharide structures covalently attached to proteins via nitrogen atoms, typically at asparagine residues. They represent one of the two major classes of protein glycosylation, alongside O-glycans.These glycans are characterized by:<ul><li>High structural diversity, arising from variations in monosaccharide composition and linkage patterns</li><li>Large size, typically comprising 7–15 monosaccharide units</li><li>Complex branching architectures, leading to a vast number of possible configurations</li></ul>Common monosaccharide components include:<ul><li>Mannose</li><li>Galactose</li><li>N-acetylglucosamine</li><li>N-acetylgalactosamine</li><li>Sialic acids</li></ul>Unlike free carbohydrates present in foods (e.g., glucose, lactose), N-glycans are protein-bound, which significantly influences their biological behavior.<h4>Biological Function and Dietary Relevance</h4>N-glycans play multiple roles in biological systems, including:<ul><li>Modulation of protein stability</li><li>Regulation of protein trafficking and localization</li><li>Facilitation of cell–cell communication and immune recognition</li></ul>They are particularly abundant on membrane and surface proteins, where they contribute to molecular signaling processes.From a dietary perspective, N-glycans exhibit unique behavior. Due to their size and protein-bound nature, they are not readily digested in the upper gastrointestinal tract. Instead, they can reach the colon, where they may function analogously to dietary fiber, interacting with the gut microbiota.<h4>Research Motivation</h4>The investigation of N-glycans in dietary proteins was initially driven by interest in protein nutrition and supplementation, particularly in the context of muscle growth and protein quality. However, this focus expanded upon recognition of the importance of glycosylation in biological systems.The field of glycomics offers:<ul><li>Extensive structural variability, enabling diverse biological functions</li><li>Relevance in disease mechanisms, including cancer, where glycosylation patterns can influence immune evasion</li><li>Opportunities to explore diet–microbiome interactions</li></ul>This combination of nutritional, biochemical, and biomedical relevance makes N-glycan research particularly compelling.<h4>Experimental Workflow for N-Glycan Analysis</h4>The analysis of N-glycans requires a multi-step workflow due to their covalent attachment to proteins.<h5>1. Protein Purification</h5>Dietary protein samples often contain:<ul><li>Free sugars</li><li>Lipids</li><li>Other matrix components</li></ul>To isolate glycoproteins:<ul><li>Cold ethanol precipitation is used to remove free carbohydrates</li><li>Multiple washing steps ensure removal of residual sugars</li><li>A phenol–sulfuric acid assay is applied to confirm the absence of free sugars</li></ul><h5>2. Enzymatic Release of N-Glycans</h5>N-glycans are enzymatically cleaved from proteins using:<ul><li>PNGase F (Peptide-N-Glycosidase F)</li></ul>This enzyme:<ul><li>Specifically cleaves the glycan from the protein backbone</li><li>Preserves the intact glycan structure</li></ul>Alternative approaches using gut-derived enzymes are being explored to improve accessibility and yield.<h5>3. Glycan Purification and Labeling</h5>Following release:<ul><li>Glycans are purified from the protein matrix</li><li>Chemical labeling is performed (e.g., 2-AA or procainamide tagging)</li></ul>Labeling enhances:<ul><li>Detection sensitivity</li><li>Chromatographic behavior</li></ul><h5>4. Analytical Techniques</h5>Two primary analytical platforms are employed:<ul><li>MALDI-TOF MS<ul><li>High sensitivity</li><li>Broad structural coverage</li></ul></li><li>HILIC-LC-MS<ul><li>Improved reproducibility</li><li>Enhanced chromatographic separation</li></ul></li></ul>These approaches provide semi-quantitative data, where signal intensity reflects relative abundance rather than absolute concentration.<h4>Analytical Challenges</h4>Several challenges are associated with N-glycan analysis:<h5>Low Abundance</h5><ul><li>Dietary proteins typically contain only 1–2% glycan content by weight</li><li>This limits yield and complicates downstream applications</li></ul><h5>Sample Preparation Complexity</h5><ul><li>Extensive purification is required to eliminate free sugars</li><li>Contamination risks must be carefully managed</li></ul><h5>Instrumentation Trade-Offs</h5><ul><li>Different analytical platforms offer complementary advantages</li><li>No single technique provides complete structural and quantitative information</li></ul><h4>Differences Between Plant and Animal N-Glycans</h4>Comparative analysis reveals distinct differences in glycan composition:<h5>Plant-Derived Proteins (e.g., soy, pea)</h5><ul><li>Predominantly high-mannose structures</li><li>Lower monosaccharide diversity</li><li>Simpler glycan architectures</li></ul><h5>Animal-Derived Proteins (e.g., whey, egg white)</h5><ul><li>Greater structural complexity</li><li>Inclusion of diverse monosaccharides (e.g., sialic acids)</li><li>More elaborate branching patterns</li></ul>These differences have important implications for biological function and microbial interactions.<h4>Implications for Gut Microbiome Research</h4>N-glycans represent a potential class of prebiotic substrates that can influence gut microbiota composition and activity.Key findings suggest:<ul><li>Structural diversity of glycans affects microbial utilization patterns</li><li>More complex glycans may promote greater microbiome diversity</li><li>Specific glycan structures may selectively stimulate beneficial microbes</li></ul>This has led to growing interest in:<ul><li>Synbiotic strategies, combining microbes with preferred substrates</li><li>Tailored dietary interventions to enhance metabolic outputs, such as short-chain fatty acids</li></ul><h4>Methodological Considerations</h4>A critical insight from this work is the importance of understanding analytical trade-offs:<ul><li>MALDI-TOF MS provides broader detection of glycan structures</li><li>LC-MS approaches offer greater reproducibility</li></ul>No single technique is universally optimal; instead, method selection should be guided by the specific research question.<h4>Conclusion</h4>N-glycans in dietary proteins represent a complex and biologically significant class of biomolecules with implications spanning nutrition, microbiology, and disease research. Advances in analytical methodologies continue to improve our ability to characterize these structures, despite challenges related to low abundance and structural diversity.A deeper understanding of N-glycan composition and function may enable the development of targeted nutritional strategies and microbiome-based therapeutics, highlighting the growing importance of glycomics in modern life sciences.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

N‑Glycans, Whey Protein, and the Gut Microbiome: Mass Spec Insights with Matthew Bolino

<div><div><div>Our immune system is incredibly powerful: among other things, it can recognize and destroy cancer cells. But tumors can be just as effective at avoiding detection – they build “invisibility shields” to protect themselves from attack.</div></div><div>One breakthrough in cancer therapy has been immune checkpoint inhibitors (ICIs), drugs that reactivate immune T cells and help them fight tumors again. However, only about 20–50% of patients respond well to this treatment. Researchers are therefore seeking alternative therapeutic strategies. A small and well-known molecule called adenosine plays a key role in these efforts.When tumor cells are stressed or dying, they release ATP, which is normally a signal that something is wrong in the tissue. But in the tumor microenvironment, ATP gets rapidly broken down by two enzymes, CD39 and CD73. Released adenosine acts like a chemical “calm-down” signal, binds to immune cells (T cells, NK cells, and others), and tells them to slow down, reducing their ability to kill cancer cells. Targeting CD73 therefore represents a promising strategy for developing new cancer treatments.</div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nove_latky_mohou_podporit_imunitni_system_v_boji_s_rakovinou_88c5e82cd5.jpeg" alt="IOCB Prague: Development of potent inhibitors to boost the immune system's ability to fight cancer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nove_latky_mohou_podporit_imunitni_system_v_boji_s_rakovinou_88c5e82cd5.jpeg 245w," sizes="100vw" width="661" height="363">At IOCB Prague, interdisciplinary teams led by Jan Řezáč, Jan Konvalinka, and Michal Hocek used a newly developed high-throughput screening method to test over 5,000 compounds, identifying two ribonucleoside derivatives as promising CD73 inhibitors.The scientists then improved the compounds’ activity by carefully modifying the ribonucleoside scaffold with bulky (het)aryl groups. Further optimization improved the pharmacokinetic properties of these compounds, yielding inhibitors with low clearance, long half-life, high solubility, and strong selectivity over related enzymes such as CD39.In cellular experiments, the compounds reduced adenosine production in cancer cells, restored T cell activation, and showed no toxicity toward human fibroblasts. The most active compound showed a single-digit picomolar inhibition constant (Ki).These findings establish a strong foundation for further in vivo efficacy and combination studies, which will explore the therapeutic potential of this novel inhibitor class.<blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsptsci.5c00707">Potent Competitive Inhibitors of Ecto-5′-nucleotidase (CD73) based on 6-(Het)aryl-7-deazapurine Ribonucleoside 5′-O-Bisphosphonates</a></h4>Ugnė Šinkevičiu̅tė, Magdalena Šímová, Radek Staník, Lenka Poštová Slavětínská, Kristýna Blažková, Pavel Šácha, Martin Lepšík, Jan Řezáč, Jan Konvalinka, Tereza Ormsby, Michal Tichý, Michal HocekACS Pharmacol. Transl. Sci. 2026, 9, 1, 191–213<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsptsci.5c00707">https://pubs.acs.org/doi/10.1021/acsptsci.5c00707</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>CD73 generates immunosuppressive adenosine in the tumor microenvironment and is a promising target for cancer immunotherapy. We have designed and systematically studied diverse 2-substituted 7-deazapurine ribonucleoside 5′-O-bisphosphonates bearing a variety of (het)aryl groups at position 6 and discovered their highly potent and selective CD73 inhibition activity. The most active compounds (with single-digit picomolar Ki) contained bicyclic (het)aryl groups at position 6 in combination with chlorine at position 2. Further optimization of pharmacokinetic properties identified inhibitors with low clearance, long half-life, high solubility, and excellent selectivity over CD39 and NTPDase3. They effectively suppressed adenosine formation in MDA-MB-231 cells, rescued CD8+ T cell activation, and were nontoxic to human fibroblasts. Overall, their profile compares favorably with AB680, a CD73 inhibitor currently in phase I/II clinical trials.</div>

Development of potent inhibitors to boost the immune system's ability to fight cancer

Article

Video

Scientists

Environment

Scientific article

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Separation Science

Agilent Technologies

Technology Networks

SCIEX

Waters Corporation

Contaminants in the sample matrix can create chromatography issues, system downtime, and unreliable test results. Having clean samples can help prevent most of these problems. There are many options for sample cleanup.
We’ll discuss the following options:
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<li>Solid phase extraction (SPE), which provides the cleanest sample.
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<li>“Targeted” filtration, which can remove specific interferences from the sample matrix and provides clean samples.
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</ul>
In this talk, we’ll discuss tips for selecting, developing, and implementing SPE methods. We’ll also discuss newly developed targeted filtration options and their benefits.
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Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
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Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.
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Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.
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Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
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Agilent Technologies: Sample Cleanup: Method Development for Solid Phase Extraction and Beyond

Sample Cleanup: Method Development for Solid Phase Extraction and Beyond

We’ll discuss tips for selecting, developing, and implementing SPE methods. We’ll also discuss newly developed targeted filtration options and their benefits.

Sample Cleanup: Method Development for SPE

Our team of experts will share tips and tricks, dive into practical recommendations and highlight selected applications such as IVT mRNA and long-read sequencing.
Here’s a glimpse of what awaits you:
<ul>
<li>Top tips for achieving high-quality analysis with the Fragment Analyzer and Femto Pulse systems
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<li>Best practices for IVT mRNA analysis using the Fragment Analyzer
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<li>Learn how to achieve a better prediction of long-read sequencing read lengths
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</ul>
Whether you’re a seasoned professional or just starting out, this event promises valuable takeaways!
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Presenter: Samina Kaufmann (Product Specialist, Agilent Technologies)
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Presenter: Leslie Friedmann (Product Manager, Agilent Technologies)
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Agilent Technologies: Back to the Lab with Your Fragment Analyzer and Femto Pulse

Back to the Lab with Your Fragment Analyzer and Femto Pulse

Top tips for achieving high-quality analysis with the Fragment Analyzer and Femto Pulse systems.

Join us for an exclusive two-day event where Agilent will showcase cutting-edge biopharma solutions.
The first day of the event we will explore Agilent’s comprehensive portfolio for the spectrum of applications from drug discovery to quality control. Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development, protein therapeutics and cell and gene therapies
The second day will focus on advanced analytical solutions, including HRMS for tRNA sequencing and LC/MS for DNA/RNA analysis. Learn how to scale up oligonucleotide purification with Agilent biocolumns, which enhance the efficiency and accuracy of your processes.
By attending this event you will:
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<li>Gain insights from Agilent to optimize biotherapeutic workflows, including rigorous quality testing in cGMP environments and handling nucleic acid, vaccines, mAB and protein samples.
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<li>Understand how metabolic analyses can impact CAR cell function and behavior, using cytolytic cell killing assays on the xCELLigence system to develop cell therapy processes and confirm product potency.
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<li>Learn about new techniques in high-resolution LC/MS for analyzing tRNA modifications.
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<li>Discover software tools available for high-resolution MS systems in both research and QA/QC labs.
</li>
<li>Compare analytical and semipreparative LC purification methods for synthetic oligonucleotides, gaining competitive insights into purification strategies.
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</ul>
<h5>Talk 1: Advanced Analytical Solutions for Nucleic Acid Analysis and Purification</h5>
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<li>Presented by Dr. Charlotte Lekieffre, Biopharma Application Specialist, Agilent Technologies</li>
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The emphasis during the second day of the Summit will be on advanced analytical solutions, such as HRMS for tRNA sequencing and LC/MS for DNA/RNA analysis. Additionally, you’ll discover techniques to enhance the efficiency and accuracy of your processes by scaling up oligonucleotide purification using Agilent biocolumns. 
<h5>Talk 2: Extracted Yeast tRNA Sequencing by LC-MS/MS</h5>
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<li>Presented by Aurore Attina, Engineer of Mass Spectrometry, University of Montpellier</li>
</ul>
RNA modifications are essential for RNA stability, maturation, and functionality; directly influencing protein translation and gene regulation. They play a central role in various biological processes and are associated with many diseases, including cancers and neurological disorders. tRNA (transfer RNA), which plays a key role in protein synthesis, is the most modified molecule in the organism known to date. tRNA modifications are essential to ensure their correct function in protein translation, maintain the accuracy and fidelity of the genetic code, and enable flexible adaptation to changing cellular conditions and are therefore a crucial attribute to monitor. To monitor and map these modifications of tRNA, we have developed a LC-HRMS analytical workflow, first using synthetic oligonucleotide for the method optimization. In this webinar, we will first go through the various steps involved in LC-MS/MS method optimization using synthetic oligonucleotides. In a second part, we will present the protocol development of an enzymatic digestion applied to tRNA extracted from yeast. This digestion generates oligonucleotide fragments that can be analyzed by LC-MS/MS for sequence confirmation.
<h5>Talk 3: Discover Agilent’s Software Tools to Confirm Oligonucleotide Sequence Integrity</h5>
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<li>Presented by Dr. Holger Stalz, LC/MS Product Specialist Austria &amp; Switzerland, Agilent Technologies</li>
</ul>
Mass spectrometry (MS) is increasingly important for the development of therapeutic modalities such as oligonucleotides. A common analytical method for large molecules is intact mass by LC/MS. This can be used for the characterization and identification of a product or process-related impurities, or simply as a confirmation of molecular weight. The newly introduced Agilent OpenLab CDS 2.8 SW version comes with a set of features to enable the automatization of biopharma workflows. If you want to know how LC/MSD Spectral Deconvolution of your biopharma samples can be automated, we will provide answers during our presentation. Even more details on Oligonucleotide sequence confirmation and impurities characterization can be acquired using the power of a high-resolution accurate mass system (HRAM) on the Agilent 6545XT AdvanceBio LC/Q-TOF. Analyzing the sample data with the software workflows in Agilent BioConfirm version 12 will support the user to get the trusted answers to their most relevant questions.
<h5>Talk 4 Maximize Your Resources and Simplify Scale-up with AdvanceBio Oligonucleotide Columns</h5>
<ul>
<li>Presented by Dr. Andrea Tripodi, Application Development Engineer, Agilent Technologies</li>
</ul>
Several classes of nucleic acids, such as antisense oligonucleotides, small interfering RNAs (siRNAs), and aptamers are being investigated for potential therapeutic applications. Synthetic oligonucleotides inevitably contain many impurities due to limitations in the efficiency of each chemical reaction used during manufacture. They include deletion sequences (missing one or more nucleotides) and reaction by-products often arising during the final deprotection or cleavage steps. Ion pair reversed-phase (IP-RP) chromatography stands out as the prevalent method for analyzing and purifying oligonucleotides. For increased resolution and separation of your full-length synthetic oligonucleotide from closely related impurities, AdvanceBio Oligonucleotide is the ideal choice. The latest developments include semi-preparative, and lab-scale preparative columns packed with superficially porous particles that provide excellent performance and resolution without compromising column loading. A fast and easy scale-up method from analytical to semi-preparative scale was successfully achieved using a 10 mm ID column enabling higher sample loading using the same analytical LC instrument.

Technology Networks: Biopharma Innovations Summit

Biopharma Innovations Summit Day 2

Learn more about our cell analysis and genomics expertise and the tools we provide to drive IVT mRNA development.

This comprehensive webinar will equip you with the knowledge and skills needed to excel in N-glycan analysis, from sample preparation to data interpretation.Enhance your understanding of the latest tools Agilent offers to address your biotherapeutic characterization needs.<h4>By attending this webinar, you will:</h4><ul><li>Understand the significance of N-glycans &nbsp;</li><li>Learn about the latest N-glycan sample preparation techniques &nbsp;</li><li>Understand how to select the right columns and perform HILIC separations &nbsp;</li><li>Analyze data with the new Glycan Peak Assignment Tool</li></ul><blockquote>Presenter: Tom Rice (Bioconsumables Product Manager, Agilent Technologies)Tom Rice is the Bioconsumables Product Manager at Agilent Technologies, coming to Agilent through the acquisition of ProZyme in 2018. Prior to joining Agilent, he held various sales and marketing roles within ProZyme beginning in 2014. Much of Tom’s work, both at ProZyme and Agilent, has been focused on bringing innovative glycobiology solutions to biopharma customers. Tom is based in California and holds a B.S. from Sonoma State University.Presenter:&nbsp;Randall Robinson (Research and Development Scientist, Chemistries and Supplies Division at Agilent Technologies)Randall Robinson is a Research and Development Scientist in the Chemistries and Supplies Division at Agilent. Prior to joining Agilent in 2021, Randall completed his PhD in food chemistry with a focus on developing analytical techniques to characterize bioactive oligosaccharides in milk and dairy streams. Since joining Agilent, he has contributed to the design of numerous products utilized for N-glycan analysis, including the HILIC column described in this presentation.</blockquote>

Separation Science: Streamlining N-Glycan Analysis: A Complete Workflow from Agilent

Streamlining N-Glycan Analysis: A Complete Workflow from Agilent

Learn about the latest N-Glycan sample preparation techniques.

Streamlining N-Glycan Analysis: A Complete Workflow

Day two: Characterization and discovery<ul><li>Characterize and quantify calreticulin (CALR) arginylation using electron-activated top-down proteomics</li><li>Achieve in-depth profiling of metabolites and lipids in biological samples</li><li>Obtain accurate and reliable metabolite identification using electron-based fragmentation (EAD)</li><li>Detect trace amounts of pesticide residues in food by targeted and non-targeted, IDA and SWATH approaches</li><li>Enhance routine characterization of low-abundant sequence variant (SV) through fast and sensitive EAD-DDA</li></ul><h5>Agenda-at-a-glance</h5>Welcome and introduction to characterization and discoveryTop-down characterization of post-translational arginylation using electron activated dissociation (EAD)<ul><li>Learn about our top-down proteomics workflow for the characterization and quantification of calreticulin (CALR) arginylation. MS1 profile and MS2 fragmentation were characterized by EAD. Preferential fragmentation at the CALR N-terminals yielded sufficient c ions facilitating precise localization of the arginylation sites. Learn about:<ul><li>Arginylation regulates CALR degradation and ER function</li><li>EAD is efficient in characterizing N-terminal arginylation</li><li>The workflow sets up the foundation for unbiased top-down activity-based arginylation profiling</li></ul></li></ul>In-depth metabolomics and lipidomics analyses<ul><li>Routine measurement of metabolites and lipids has become a cornerstone of biomedical research. At the MPC, Helmholtz Munich, non-targeted analysis is based on the SCIEX ZenoTOF 7600 for accurate and in-depth analysis and annotation of metabolites and lipids from different biological specimens.<ul><li>Application of Zeno trap enabled non-targeted workflows to large scale metabolomics and lipidomics studies</li><li>Enhanced sensitivity enables deeper profiling of biological samples</li><li>Increased confidence in identifications through comprehensive structural elucidation using Electron activated dissociation (EAD)</li></ul></li></ul>MetID innovations in pharma: exploring new avenues for metabolite structure elucidationTime-of-flight mass spectrometry applications in food science: targeted and non-targeted approaches<ul><li>Time-of-flight mass spectrometry has become a key tool in analytical laboratories involved in food analyses. This instrumentation can be used for both targeted and non-targeted approaches. In this presentation, we will discuss:<ul><li>Overview of practical targeted and non-targeted approaches in food analysis</li><li>Application of a SWATH® approach to the analysis of pesticide residues in food</li><li>Application of IDA approaches in non-targeted food analyses</li></ul></li></ul>Reduce risk and accelerate cell line development with iCIEF-UV/MS technology<ul><li>Guide decisions during cell line development with the Intabio ZT system with more information on how process changes affect cell line and product stability. &nbsp;<ul><li>How to utilize icIEF-UV/MS to identify sequence variants</li><li>How this information can be used to create a mitigation strategy</li><li>What more information in less time means to groups throughout process development</li></ul></li></ul>Q&amp;A roundtable: A deeper look at high-resolution mass spectrometry<ul><li>Moderated by: ose Castro-Perez, Vice President of Product Management, SCIEX</li><li>Panelists include:<ul><li>Tom Lin, Instructor, Washington University in St. Louis</li><li>Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz Munich</li><li>Francisco José Díaz-Galiano, Postdoctoral researcher, University of Almería</li><li>Kirsten Nields, Principal Scientist, J&amp;J Innovative Medicines</li><li>Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX</li></ul></li></ul><blockquote>Moderator: Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEXTatjana is a Senior Product Manager at SCIEX, overseeing accurate mass systems since 2021. She collaborates with R&amp;D, sales, and marketing teams to ensure effective project execution and portfolio management.Previously, Tatjana was a Product Manager at MOBILion Systems, leading the pre-commercialization of SLIM-based ion mobility instruments. She also has held roles at Thermo Fisher Scientific as a Sr. Vertical Marketing Specialist and Sr. Field Applications Scientist.Tatjana holds a B.A. in chemistry from Austin College and a Ph.D. in biomedical sciences and biochemistry from the University of North Texas Health Science Center.Jamie holds a B.Sc. in engineering physics from Queen’s University.Presenter: Tom Lin, Instructor, Washington University in St. LouisPresenter: Michael Witting, Deputy Head Metabolomics and Proteomics Core and Executive Manager Metabolomics, Helmholtz MunichPresenter: Francisco José Díaz-Galiano, Postdoctoral researcher, University of AlmeríaPresenter: Kirsten Nields, Principal Scientist, J&amp;J Innovative MedicinesPresenter: Tatjana Talamantes, Senior Product Manager, Accurate Mass, SCIEX</blockquote>

SCIEX summit Day two: Characterization and discovery

Importance of LC-MS in food and environmental testing, pharmaceutical and biopharmaceutical discovery and development and biomedical and omics research.

CIEX summit Day two: Characterization and discovery

UV-Vis spectroscopy is a cornerstone analytical technique in the pharmaceutical industry, widely employed for its versatility and reliability serving essential functions such as the synthesis of drug compounds, purity assessment, dissolution profiling, and the analysis of biomolecules throughout the drug development process. This technique is pivotal for ensuring the quality and efficacy of pharmaceutical products. However, the pharmaceutical industry faces several challenges with traditional UV-Vis spectroscopy, such as the need for stringent regulatory compliance, the demand for high precision and accuracy, and the requirement for efficient data management systems to handle the vast amount of data generated.The Agilent Cary 3500 UV-Vis model addresses these pain points effectively, join the webinar to discover how this cutting-edge instrument elevates the standards of pharmaceutical analysis with its unique features and seamless OpenLab integration.Key Takeaways:<ul><li>Innovative Temperature Control: Learn how the Cary 3500’s precise temperature control accelerates your analytical processes.</li><li>Rapid Data Acquisition: Understand the impact of high-speed data collection on your research outcomes.</li><li>Small-Volume Mastery: Explore the instrument’s proficiency in small-volume analysis, ensuring accuracy down to the minutest quantities.</li><li>Highest Absorbance Range: Explore the Cary 3500’s superior absorbance range for unparalleled sensitivity in your measurements.</li><li>OpenLab Server Connectivity: Experience the seamless data management and integrity that OpenLab server-based connectivity provides, meeting stringent regulatory compliance.</li><li>Interactive Q&amp;A Session: Engage with our experts to address your queries and discuss the instrument’s application in real-world pharmaceutical scenarios.</li></ul><blockquote>Presenter: Ms. Vijaya Jyothi (Product Specialist - Spectroscopy, Agilent Technologies)Vijaya Jyothi is a seasoned product specialist at Agilent Technologies. She manages the spectroscopy division, having extensive expertise in spectroscopy applications across diverse industries, offering deep insights and solutions tailored to varied analytical needs.</blockquote>

Agilent: Cary 3500 UV-VIS Advanced Analytical Capabilities and Seamless Server Connectivity

Cary 3500 UV-VIS Advanced Analytical Capabilities and Seamless Server Connectivity

Discover how this cutting-edge instrument elevates the standards of pharmaceutical analysis with its unique features and seamless OpenLab integration.

Cary 3500 UV-VIS Advanced Capabilities

<h4>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</h4>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.<h5>Why attend?</h5><ul><li>Comprehensive Learning: Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li>Expert Insights: Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li>Regulatory Compliance: Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3>Episode #6: Automated Sample Preparation | F&amp;E Summer School - Day 2 - Module 2</h3>This Module builds on the previous one by adding automation to the sample preparation methods. The Andrew+™ pipetting robot offers fully automated liquid handling using electronic pipettes and labware holders called Dominos for a full walk-away automation solution.Creating the connected laboratory, Andrew+ is part of the OneLab™ ecosystem, executing laboratory protocols designed in OneLab software. This flexible architecture enables you to effortlessly transition from laborious manual pipetting procedures to error-free, fully-robotized lab workflows – without any knowledge of programming, laboratory robotics or automation engineering. and explains processes the Andrew+ pipetting robot can take over in your lab.The European Union has adopted Commission Recommendation (EU) 2022/1431 to monitor PFAS in fruits, vegetables, milk and baby food. To achieve quantification at lowest levels of PFAS in food matrices, sample preparation is required which comprise of many pipetting steps and can be time-consuming and error prone. We will demonstrate an automate workflow for the analysis of PFAS in milk using a pipetting robot and stacked dual-phase SPE cartridges to meet EU 2022/1431 levels.<h5>What you'll learn:</h5>Possibilities to automat liquid handling processes in LC and LC-MS e.g.<ul><li>Serial dilution</li><li>Calibration curve</li><li>Solid Phase Extraction (SPE)</li></ul><blockquote>Presenter:&nbsp;Xavier Rodríguez (Business Development Manager Chemistry Group, Southern Europe, Waters)Xavier is part of the European Market Development Team. He works as an Application Specialist with focus on sample prep and has more than 20 years of experience in solving sample prep challenges.Presenter:&nbsp;Daniel Ng PhD (Principal Scientist, Waters Corporation)Daniel Ng received his PhD from the University of Manchester in mass spectrometry proteomics. He worked for 5 years at the Waters mass spectrometry headquarters in Wilmslow, UK, as a service support development engineer specializing in mass spectrometry instrumentation. In 2019, he took up a new role as a principal scientist at the International Food and Water Research Centre (Waters) in Singapore focusing on using advanced instrumentation to answer analytical challenges in the food and water industry. Since 2023, Daniel has lead the Food, Environmental and Material Science Analytical Professional Service team in the Asia-Pacific Region, developing analytical solutions to meet key challenges that customers face.</blockquote>

Waters Corporation: The Waters 2024 Virtual Summer School

Automated Sample Preparation

The Andrew+™ pipetting robot offers fully automated liquid handling using electronic pipettes and labware holders.

<h4>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</h4>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.<h5>Why attend?</h5><ul><li>Comprehensive Learning: Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li>Expert Insights: Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li>Regulatory Compliance: Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3>Episode #7: Quantification for Targeted Analysis | F&amp;E Summer School - Day 2 - Module 3</h3>MS Quan is an application in the waters_connect™ for Quantitation software platform that is designed to streamline the LC-MS/MS data processing and review with innovative workflows, data visualization and data navigation. The MS Quan application’s main workflows and overarching features such as task-oriented workflow, batch-level review, and exception-focused review are introduced and illustrated with a PFAS analysis dataset.<h5>What you'll learn:</h5><ul><li>How to create processing method</li><li>Data review with exception focus review</li><li>Compare data and evaluate peak integration</li><li>Export and report of final results</li></ul><blockquote>Presenter:&nbsp;Helge Grunwald (Sr Sales Applications Chemist, Waters Corporation)Helge is part of the Customer Success team at Waters with a focus on LC-MS/MS application and sales support. He works in this role for more than 4 years and gained experience in previous roles as project manager at Intertek and method developer at Basilea Pharmaceutica after he finished his Master of Science in Life Science at FH Nordwestschweiz.</blockquote>

Quantification for Targeted Analysis

The MS Quan application’s main workflows and overarching features are introduced and illustrated with a PFAS analysis dataset. 

<h4>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</h4>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.<h5>Why attend?</h5><ul><li>Comprehensive Learning: Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li>Expert Insights: Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li>Regulatory Compliance: Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3>Episode #9: PFAS in Food | F&amp;E Summer School - Day 3 - Module 1</h3>The European Commision published for the first time maximum levels for the so-called “EU 4” PFAS (PFHxS, PFOS, PFOA and PFNA) in eggs, meat and fish with Regulation EU 2023/915. This was an expected result of EFSA’s letter of concern on those 4 PFAS and labs reacted accordingly. A more challenging task came with the EURL PFAS Guidance Document and a bit later with the Recommendation EU 2022/1431. The target LOQs for the EU 4 PFAS were set at 0.001 µg/kg in fruits, vegetables and babyfood. We will discuss the successful method implementation from sample preparation to final report and hear real-life experiences from our guest speaker Victoria Erdely (Eurofins GfA) and Katharina Rund (EURL PFAS).<h5>What you'll learn:</h5><ul><li>How to reach lowest levels of PFAS in food and work according to Recommendation EU 2022/1431 using Xevo TQ Absolute in combination with UniSpray ion source</li><li>How LC method development on Premier LC gives you the widest PFAS panel and separates interferences.</li><li>Tips &amp; tricks from the demo lab to avoid PFAS contamination, implement new analytes, speed up data processing, and keep best performance of your system</li><li>About a special PFAS training to help you implement the method in the lab.</li></ul><blockquote>Presenter:&nbsp;Dr. Sara Stead (Consulting Scientist, Food and Environmental, Scientific Operations, Waters Corporation)In her current position, Sara is a Principal Sales Application Chemist specializing in food and environmental LC-MS/MS based applications within the Waters UK and Ireland demo laboratory. Sara has worked in F&amp;E analysis with various research interests including the development of both multi-residue HRMS screening and targeted LC/GC MS based methods for the past 25 years in both the government and private sectors. ​Prior to joining Waters Sara was employed as a Lead Scientist at the Food and Environment Research Agency (FERA, Defra, UK) for 14 years. Sara has participated in EU Food and Veterinary Office (FVO) audit inspections, was an expert on behalf of the International Atomic Energy Agency (IAEA) and a member of the EuroResidue conference scientific committee.Presenter:&nbsp;Dr. Stuart Adams (Principal Scientist, Market Development, Food and Environmental Applications, Europe, Waters Corporation)Stuart has been developing methods for the determination of contaminants in food and the environment for over 17 years and has been with Waters Corporation since 2018. Stuart's previous role was as a senior analytical chemist at Fera (the UK’s National Reference Laboratory) and moved to Waters to focus on application development in the European region. He now serves as the attached scientist to the European Market Development team responsible for the food and environmental application areas. In his role, he works with customers to understand their analytical challenges and helps address them with Waters technologies.Presenter:&nbsp;Victoria Erdely (Head of Department - Method and Instrument Support, Eurofins GfA)Presenter:&nbsp;Dr. Katharina Rund (Food Chemist, EU Reference Laboratory for halogenated POPs in Feed and Food)</blockquote>

PFAS in Food

We will discuss the successful method implementation from sample preparation to final report and hear real-life experiences.

<h4>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</h4>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.<h5>Why attend?</h5><ul><li>Comprehensive Learning: Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li>Expert Insights: Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li>Regulatory Compliance: Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3>Episode #10: PFAS Analysis in Drinking, Ground, and Surface Water | F&amp;E Summer School - Day 3 - Module 2</h3>Analysis of PFAS is an essential part of water testing programs and becoming a regular requirement for monitoring in environment and food sources. As regulations, like the EU Drinking Water Directive, the EU Groundwater Directive, and the EU Water Framework Directive, have been updated or are in the process for an update, the requirements for method sensitivity have become more demanding. Whilst concentration levels go down, direct injection methods are increasingly desirable to laboratories carrying out routine analysis of PFAS where analyte preconcentration is not deemed necessary or required. They have a simple sample preparation method, which not only reduces the opportunity for contamination and error but speeds up analysis time and the requirements for large volumes of samples. This can provide labs with a higher throughput of samples and lower analysis costs.Dorrit Griesshaber from the Office for Environment and Energy in Basel (SZ) and Jenny Davies from Element Materials Technology in Flinshire (UK) will give insights how the analysis of PFAS in environmental samples works in routine testing labs.<h5>What you'll learn:</h5><ul><li>How to reach lowest levels of PFAS and work according to EU regulation using Xevo TQ Absolute in combination with UniSpray ion source,</li><li>LC method development on Premier LC and system modification for direct injection with large volume,</li><li>Tips &amp; tricks from the demo lab to avoid PFAS contamination, implement new analytes, speed up data processing, and keep best performance and</li><li>A special PFAS training to help you implement the method in the lab.</li></ul><blockquote>Presenter:&nbsp;Dr. Sara Stead (Consulting Scientist, Food and Environmental, Scientific Operations, Waters Corporation)Presenter:&nbsp;Aron Dekkers (Sales Application Chemist, Waters Corporation)Aron is part of the Customer Success team at Waters BeNeLux with a focus on Food &amp; Environmental LC-MS/MS applications and sales support. His special interest areas are PFAs and pesticides. He works in this role for more than 4 years. Aron finished his B.S. in Chemistry at Fontys Eindhoven (NL).Presenter:&nbsp;Dorrit Griesshaber (Amt für Umwelt und Energy (AUE))Presenter:&nbsp;Jenny Davies (Technical Director, Element Materials Technology)</blockquote>

PFAS Analysis in Drinking, Ground, and Surface Water

We will give insights how the analysis of PFAS in environmental samples works in routine testing labs. 