In today’s academic research environment, speed and precision are everything. Whether you're analyzing trace organic compounds, processing environmental extracts, or running a high-throughput metabolomics project, one bottleneck continues to slow down even the most well-equipped labs: solvent evaporation.If your lab still relies on aging hot plates or hand-drying methods, you’re not alone. But the consequences are real: inconsistent recovery, lost analytes, and valuable hours wasted each week.<h3>The Hidden Cost of Manual Evaporation</h3>Many academic labs were built on traditional sample prep methods. But as research needs evolve and sample volumes increase, these outdated tools can no longer keep up.Manual evaporation doesn’t just slow workflows—it undermines reproducibility.Hot plates can over-dry samples or introduce uneven heating that degrades sensitive compounds. Open evaporation risks contamination. And when multiple students share equipment, timing differences from sample to sample introduce variability that shows up later as noisy chromatography or inconsistent quantitation.Students and faculty alike often report frustration with drying steps taking far too long, especially when preparing dozens of samples for <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis?hsLang=en">LC-MS</a> or <a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis?hsLang=en">GC-MS workflows</a>. In some cases, samples left unattended can be partially lost or require re-preparation entirely.For grant-funded research, that wasted time and material adds up quickly.<h3>A Better Way: Controlled Nitrogen Blowdown</h3>Nitrogen blowdown evaporation replaces guesswork with control. A steady stream of inert nitrogen combined with gentle, regulated heat speeds evaporation while protecting sensitive analytes.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=362&amp;manufacturerItems=540">Systems from Organomation </a>are designed specifically for sample preparation—not as improvised solutions or general lab accessories, but as purpose-built tools for consistent concentration.The result is straightforward:<ul style="list-style-type:disc;"><li>Faster dry-downs</li><li>Better recoveries</li><li>Improved reproducibility</li><li>Less hands-on time</li></ul>So researchers can focus on generating data instead of babysitting samples.<h3>Why Academic Labs Choose the N-EVAP</h3>Research labs rarely process identical samples all day.One student may be concentrating a small organic extract, another working with aqueous environmental samples, and a third reducing a larger crude matrix—all at the same time. That variability is exactly where many batch systems fall short.The<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=362&amp;manufacturerItems=540&amp;search=n-evap"> N-EVAP</a> transforms sample preparation uncertainty into control through radical flexibility.Unlike systems that force you to work around equipment constraints, the N-EVAP’s individual per-sample valves let you adjust gas flow, temperature exposure, and needle positioning for each sample—simultaneously.This isn’t theoretical flexibility. It means you can:<ul><li>Concentrate a 10 mL aqueous sample</li><li>Reduce a 5 mL organic extract</li><li>Evaporate a 50 mL environmental sample</li></ul>—all in the same run, without compromise.<blockquote><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/manual-to-automated-solvent-drying-is-it-worth-the-switch">Learn how the University of Cincinnati achieved 20x faster sample prep with the N-EVAP evaporator.</a></blockquote><ul><li>For academic programs handling variable matrices, this eliminates the false choice between batch efficiency and analytical precision. Each sample gets exactly the conditions it needs, preserving analyte integrity while maintaining throughput. &nbsp;</li><li>Available in configurations from <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2352">6</a> to <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1911">45 </a>positions—with optional digital controls on larger models—the system scales easily from small teaching labs to large research groups without forcing workflow changes. &nbsp;</li><li>It’s built for scientists who want control over their experiments, not workarounds. &nbsp;</li><li>Our evaporators are the <a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/why-organomation-is-the-most-cited-blowdown-evaporator-brand-in-u.s.-epa-methods">most cited nitrogen blowdown sample concentrators</a> in standardized EPA methods, making Organomation the trusted, fail proof choice.</li></ul><h3>Built for Chromatography Workflows</h3>Since 1959, Organomation has focused exclusively on sample preparation. That specialization shows in the details that matter most to chromatography labs: uniform heating, consistent gas distribution, compatibility with common vial formats, and rugged construction that stands up to daily use in shared academic spaces.Whether you're preparing samples for LC-MS, GC-MS, or other analytical methods, nitrogen blowdown helps ensure:<ul><li>Faster turnaround between experiments</li><li>Consistent recoveries</li><li>Fewer re-runs</li><li>Less student labor tied up in drying steps</li></ul>Which ultimately means more time spent on analysis, interpretation, and publication.<h3>Prove the Time Savings for Yourself</h3>Upgrading your evaporation workflow can clearly improve efficiency—but how much time would your lab actually save? ➔ Rather than guessing, you can calculate it.Organomation’s free <a target="_blank" rel="noopener noreferrer" href="https://explore.organomation.com/evaporation-time-calculator-excel-download">Evaporation Time Savings Calculator</a> lets you enter your real lab conditions—sample counts, volumes, current dry-down times, and run frequency—to quantify exactly how much time nitrogen blowdown could save each week, month, or semester.For many academic labs, the results are eye-opening:<ul><li>Hours of student and staff time reclaimed</li><li>Faster LC-MS/GC-MS queues</li><li>Reduced bottlenecks before analysis</li><li>Hard numbers that help justify equipment purchases in grants and budgets</li></ul>In just a few minutes, you’ll have concrete data showing whether flexible evaporation makes financial and workflow sense for your lab.<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://explore.organomation.com/evaporation-time-calculator-excel-download">Download the Evaporation Time Savings Calculator and see what your lab could save.</a></blockquote>

Is Your University Lab Losing Time and Data in Sample Prep? Here’s How to Fix It

<h4>The science behind gummy candies</h4>Who can resist colourful, fruity gummy candies 🍬 with their signature chewy and elastic texture? While they may appear simple, their formulation is actually quite sophisticated. Each gummy is produced using a precisely balanced combination of gelling agents, sugars, acids, flavourings, and colourants, all working together to deliver the desired taste and texture.For instance, the characteristic chewiness of gummy sweets results from the interaction between gelatin and sugars. Even minor adjustments—such as changing the type or concentration of the gelling agent—can significantly alter the final product. In addition, manufacturers must meet regulatory and labeling requirements, including certifications like Halal compliance. This makes accurate and consistent monitoring of ingredients essential throughout production.<h4>The importance of SEC fingerprinting</h4>Size Exclusion Chromatography (SEC), also known as Gel Permeation Chromatography (GPC), offers a fast and reliable way to analyze gummy formulations. By separating molecules according to their hydrodynamic size, SEC generates a unique molecular “fingerprint” for each product.This fingerprint enables manufacturers to:<ul><li>confirm the type and origin of gelling agents (e.g., for Halal verification)</li><li>ensure batch-to-batch consistency and detect production variations</li><li>monitor changes during storage to assess shelf life</li><li>evaluate low-molecular-weight compounds such as sugars in a semi-quantitative manner</li></ul>Overall, SEC fingerprinting supports consistent product quality and regulatory compliance, ensuring that every gummy maintains the same expected characteristics.<h4>Influence of column selection on SEC performance</h4>Column selection plays a crucial role in achieving high-quality separations and detailed molecular fingerprints. To illustrate this, porcine and bovine gelatin standards were analyzed using two different configurations:<ul><li>a single Multipore <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1655">SuperOH-P column</a></li><li>a coupled system of SuperOH-P 350 and SuperOH-P 150 columns</li></ul>Although both setups cover the same molecular weight range (100–1,000,000 Da), the coupled configuration effectively doubles the separation length. This results in improved resolution, which was confirmed experimentally. The coupled columns provided clearer separation between porcine and bovine gelatin, producing more distinct and informative fingerprints.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_1_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_d81181cfb4.jpg" alt="KNAUER: Fig. 1 Overlay chromatograms measured with DAD at 230 nm, 20 µL. Dark blue: porcine reference standard; red: bovine reference standard; solid line: measured with column A; dotted line: measured with column set B. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_1_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_d81181cfb4.jpg 245w," sizes="100vw" width="1024" height="611"><h4>Achieving reliable SEC fingerprints</h4>Reproducibility is key when using SEC for quality control. The process begins with consistent sample preparation—maintaining identical dissolution procedures, concentrations, and filtration steps is essential, as even small variations can influence peak shape and overall results.Beyond the column, the entire system setup is equally important. For example, combining a Refractive Index Detector (RID) with a Diode Array Detector (DAD) allows simultaneous detection of sugars, acids, and polymers within a single analysis.Accurate peak identification further enhances reliability. By analyzing reference standards based on known ingredient compositions, chromatographic peaks can be correctly assigned, enabling detailed interpretation of both high- and low-molecular-weight components.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_2_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_37caf5ac59.jpg" alt="KNAUER: Fig. 2 Zoom overlay chromatograms measured with RID, 20 µL and column set B. Black: Katjes® Yoghurt Gums; turquoise: apple pectin diluted 1:10; dark green: preserving sugar 1:1 (sugar; citric acid; pectin; fully hydrogenated sunflower oil); dark blue: maltodextrin; pink: sodium potassium tartrate; yellow: potassium citrate; red: citric acid; purple: lactic acid; beige: lactose; orange: sucrose; green: glucose; grey: blank. Graphic by KNAUER. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_2_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_37caf5ac59.jpg 245w," sizes="100vw" width="1024" height="611">Additionally, incorporating a flow marker helps correct for system-related variations, such as fluctuations in flow rate, thereby improving retention time stability and peak assignment accuracy. More details on the flow marker and its use in SEC are described in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0043_knauer_gpc_sec_data_reproducibility_with_flow_marker_96ee6b3cd9.pdf">this technical note</a>.<h4>Verification of gelatin origin using SEC</h4>Determining the source of gelatin is critical for proper labeling and compliance, particularly for Halal-certified products where porcine gelatin is not permitted.To demonstrate SEC’s capability, two gummy samples with identical formulations but different gelatin sources were analyzed. One sample contained porcine gelatin, while the other used bovine gelatin. The resulting SEC fingerprints showed clear differences in the gelatin region, allowing unambiguous identification. The standard Haribo® Goldbears corresponded to the porcine reference, whereas the Halal version matched the bovine standard.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_3_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_and_column_set_B_12e99a43e7.jpg" alt="KNAUER: Fig. 3 Overlay chromatograms measured with DAD at 230 nm, 20 µL and column set B. Solid dark blue line: Haribo® Goldbears; solid red line: Halal Haribo® Goldbears; dotted dark blue line: porcine reference standard; dotted red line: bovine reference standard. Graphic by KNAUER. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_3_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_and_column_set_B_12e99a43e7.jpg 245w," sizes="100vw" width="1024" height="611"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_4_Overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_2_c29ea59bc4.jpg" alt="KNAUER: Fig. 4 Overlay chromatograms measured with RID, 20 µL and column set B. Solid dark blue line: Haribo® Goldbears; solid red line: Halal Haribo® Goldbears; dotted dark blue line: porcine reference standard; dotted red line: bovine reference standard. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_4_Overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_2_c29ea59bc4.jpg 245w," sizes="100vw" width="1368" height="432">This confirms that SEC is a reliable method for distinguishing gelatin origin.<h4>Role of low-molecular-weight components</h4>In addition to polymers such as gelatin, SEC can also reveal smaller molecules, including sugars and organic acids. While SEC is not a replacement for fully validated HPLC methods, it serves as a rapid screening tool for comparing samples and supporting quality control.Using RID detection, differences in sugar composition become visible. For example, chromatograms showed a significantly higher sucrose signal in preserving sugar compared to gummy candies, reflecting formulation differences.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_5_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_06c072e069.jpg" alt="KNAUER: Fig. 5 Zoom overlay chromatograms measured with RID, 20 µL and column set B. Black: sucrose 1mg/ml; blue: Haribo® Goldbears; green: preserving sugar 1:1 (sugar; citric acid; pectin; fully hydrogenated sunflower oil). Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_5_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_06c072e069.jpg 245w," sizes="100vw" width="1024" height="611"><h4>Conclusion</h4>SEC is a powerful analytical technique for monitoring product quality and ensuring process consistency in gummy candy production. By generating detailed molecular fingerprints, it enables manufacturers to verify ingredients, maintain consistency, and comply with regulatory requirements.Importantly, this approach is not limited to gummy candies—it can be adapted to a wide range of products where molecular characterization is essential.<blockquote><ul><li>If you're planning to set up or optimize your GPC/SEC workflow, feel free to contact us at sales@knauer.net. Stay tuned for more exciting insights into the GPC/SEC world in our “GPC/SEC Made Easy” series.</li><li>For more information on the technical details, you can refer to <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VFD_0195_sec_for_fingerprint_analysis_of_gummy_candies_ce5349cd95.pdf">this application note.</a></li><li>For further information on this topic, please contact our author: wesolowski@knauer.net</li></ul></blockquote>

GPC/SEC Made Easy – Fingerprint analysis of Gummy Candies

News from LabRulezLCMS Library - Week 17, 2026

Article

Product

Application

Food

Science and research

Laboratories

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Metrohm

Agilent Technologies

With the addition of MS detection to HPLC analyses, certain considerations must be made in selecting columns and conditions.
In this seminar we will:
<ul>
<li>Discuss the fundamentals and applications of various LC/MS techniques.
</li>
<li>Look at column choices and mobile phase selection for LC separations as well as for MS sensitivity
</li>
<li>Address specific challenges to LC/MS analyses, such as interferences and ion suppression, will be addressed.
</li>
<li>The importance of sample preparation in minimizing problems with interferences will also be covered.
</li>
</ul>
<blockquote>
Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.
</blockquote>

Agilent Technologies: Making LC Methods MS Friendly

Making LC Methods MS Friendly

With the addition of MS detection to HPLC analyses, certain considerations must be made in selecting columns and conditions.

If you’re doing food testing and want to find ways to do more with less while getting consistent accurate results this seminar is for you.
We will walk through a sample application – multi-residue pesticides in fish using LC/MS – from sample prep to data analysis, and point out tips for saving both time and money and improving results. Key topics covered will be column selection, tips for extending column life and “just enough” sample prep solutions that reduce time spent on sample prep.
<blockquote>
Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.
</blockquote>

Agilent Technologies: New:Troublefree Food Testing - Technologies to Improve Uptime and Productivity

Troublefree Food Testing - Technologies to Improve Uptime and Productivity

Sample application – multi-residue pesticides in fish using LC/MS.

Technologies to Improve Uptime and Productivity

Very polar compounds have poor retention in reversed phase and can be challenging to separate.
In this talk, we will discuss changes to your reversed phase conditions that can help. We’ll also look at the benefits of choosing a more polar stationary phase.
If that doesn’t work you may need to explore other types of chromatography.
Some of those options are:
<ul>
<li>HILIC
</li>
<li>Ion Pair
</li>
<li>Normal Phase
</li>
</ul>
Advantages and disadvantages of these options will be discussed.
<blockquote>
Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.
</blockquote>

Agilent Technologies: Too Polar for Reversed Phase - What do you do?

Too Polar for Reversed Phase - What do you do?

Changes to your reversed phase conditions that can help.

How the chromatographic process works is not magic.
It is described by a few key parameters, such as resolution, retention factor, and selectivity. Understanding the equations that define these parameters can make them less intimidating.
In this talk we will discuss:
<ul>
<li>What they are and where they come from
</li>
<li>Their importance
</li>
<li>The effect they have on our chromatography
</li>
<li>When they don’t matter
</li>
</ul>
A better grasp of this key information helps make the separation work for us.
<blockquote>
Presenter: Bill Champion (Application Engineer, Agilent Technologies, Inc.)
</blockquote>

Agilent Technologies: Demystifying the Chromatographic Process

Demystifying the Chromatographic Process

How the chromatographic process works is not magic.

Developing reversed-phase chromatographic methods can often be both more efficient and made better by use of a systematic approach rather than a “random walk”.
Combining simple experimental design with chromatographic understanding can help us better understand how the chromatographic parameters affect the separation.
In this talk, we will discuss the use of simple experimental design to optimize our method development and how it can:
<ul>
<li>Help make the method more robust</li>
<li>Help us understand and predict chromatographic behavior</li>
<li>Make it possible to know which parameters are really important</li>
</ul>
Most of these experiments can be set up to run in a simple overnight sequence.
<blockquote>
Presenter: Bill Champion (Application Engineer, Agilent Technologies, Inc.)
</blockquote>

Agilent Technologies: Systematic Approach vs. Random Walk - Improving the Efficiency of Method Development and Optimization

Systematic Approach vs. Random Walk - Improving the Efficiency of Method Development and Optimization

Use of simple experimental design to optimize our method development.

HPLC Method Development and Optimization Basics

Whether you develop HPLC methods, run QC samples or perform contract analyses, longer analysis times cost more and FAST LC columns can help you reduce those costs.
In this seminar we will look at:
<ul>
<li>Easy-to-follow steps for converting existing methods
</li>
<li>Developing new, faster methods on existing equipment
</li>
<li>System improvements and adjustments, like flow path connections and detector acquisition rates that can help you get the best performance with your existing system.
</li>
</ul>
We’ll also discuss when and why to consider an enhanced UHPLC systems.
<blockquote>
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>

Agilent Technologies: High Resolution Fast LC - Easier Than You Think with Simple Changes

High Resolution Fast LC - Easier Than You Think with Simple Changes

Developing new, faster methods on existing HPLC equipment.

Easier Than You Think with Simple Changes

Operating an HPLC can be overwhelming to a new user.
There are many pitfalls or “gotchas”. Even the issue of what tubing and fittings are compatible and which are not can be a serious impediment to successfully operating the HPLC.
In this talk, we will discuss:
<ul>
<li>The importance of connections
</li>
<li>Mobile Phase considerations
<ul>
<li>Quality
</li>
<li>Preventing microbial growth
</li>
<li>Sample/solvent compatibility
</li>
</ul>
</li>
<li>Column Characteristics; Their importance and limitations
</li>
<li>The importance of sample prep
</li>
</ul>
Avoiding these pitfalls can help you achieve long-term, reliable results.
<blockquote>
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>

Agilent Technologies: New to HPLC? Tips for Avoiding Beginner Pitfalls

New to HPLC? Tips for Avoiding Beginner Pitfalls

What tubing and fittings are compatible and which are not can be a serious impediment to successfully operating the HPLC.

GPC/SEC, Gel Permeation Chromatography or Size Exclusion Chromatography, is a non-interactive chromatographic technique that separates analytes based on their size in solution.
It is often used in laboratories for determination of a polymers&#39; molecular weight distribution but has many other uses.
In this talk we will:
<ul>
<li>Review GPC theory
</li>
<li>Discuss why molecular weight distribution is important
</li>
<li>Look at the different types of columns and their uses
</li>
<li>Cover key column selection criteria
</li>
<li>Show how key parameters like concentration, particle size, and injection volume can affect your separation
</li>
</ul>
We will also look at examples of common mistakes and pitfalls relating to solvent choice, calibration standards, and combining columns in series.
<blockquote>
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
</blockquote>

Agilent Technologies: Gel Permeation Chromatography Basics and Beyond

Gel Permeation Chromatography Basics and Beyond

GPC/SEC, Gel Permeation Chromatography or Size Exclusion Chromatography.

The technique of Gel Permeation Chromatography is conceptually simple: It is a separation based on size where no enthalpic interactions are observed.
In practice, GPC is often much more involved yielding involved method development and data that can be difficult to interpret. Though GPC can seem mystifying at times 90% of the success is achieved by using the proper columns for your application.
During this e-seminar we will discuss what is new in GPC column technology, different types of stationary phases, and how to choose the best columns which yield:
<ul>
<li>Improved resolution
</li>
<li>Improved reproducibility
</li>
<li>Improved accuracy
</li>
<li>Improved analysis time
</li>
</ul>
The goal of the e-seminar is to illustrate how proper GPC column selection can make the difference between good and great chromatography.
<blockquote>
Presenter: Michael Kruger (Application Engineer, Agilent Technologies, Inc.)
</blockquote>

Agilent Technologies: Achieving Superior GPC Results Through the Correct Column Selection

Achieving Superior GPC Results Through the Correct Column Selection

What is new in GPC column technology, different types of stationary phases, and how to choose the best columns.

Achieving Superior GPC Results - Correct Column Selection

Karl Fischer (KF) titration is an industry standard method for measuring water content in all types of substances.
However, for various reasons some substances are not suitable for a direct Karl Fischer titration:
<ul>
<li>They release their water only slowly
</li>
<li>The water is released at high temperature only
</li>
<li>The substances are poorly soluble in KF solvents
</li>
<li>They undergo side reactions with KF reagents
</li>
<li>They contaminate the titration cell or the electrodes
</li>
</ul>
By using the KF oven technique, the above-mentioned problems can be avoided. This makes the KF oven technique a perfect solution for the analysis of difficult samples.
By viewing this webinar you will learn…
<ul>
<li>how to analyse difficult samples
</li>
<li>how to properly prepare your titration system with KF oven
</li>
<li>how to prepare your sample
</li>
<li>how to select the optimal heating temperature
</li>
<li>which reagents and which water standards you should use to obtain accurate results
</li>
</ul>
After viewing this webinar you will know how to perform accurate titrations using the Karl Fischer oven technique. Additionally, the speakers will reveal tips and tricks to help you in the daily routine. The webinar will finish with live Q&amp;A session.
<blockquote>
Presenter: Michael Margreth (Senior Product Specialist Titration, Metrohm International Headquarters, Switzerland)
</blockquote>
<blockquote>
Michael Margreth studied chemistry at the University of Applied Science in Muttenz, Switzerland. He joined Metrohm in 2005 as an application specialist and gained titration experience in the application laboratory. Since 2008 he mainly focuses on Karl Fischer titration and the determination of water.
</blockquote>
<blockquote>
Presenter: Agnieszka Kossakowska (HYDRANAL Marketing Specialist, Honeywell Research Chemicals)
</blockquote>
<blockquote>
Agnieszka Kossakowska joined Honeywell in March 2016 as a marketing specialist providing technical support and onsite seminars for Honeywell’s Hydranal™ reagents for Karl Fischer titration. Agnieszka has more than 10 years of experience in analytical chemistry and conducts training across Europe, Middle East &amp; Africa, Latin America and Asia.
</blockquote>

Metrohm: Tips and tricks to analyze difficult samples using the Karl Fischer oven technique