Ultrashort chain per- and polyfluoroalkyl substances (USC-PFAS),defined as those with three or fewer carbon atoms, are characterisedby their significant mobility within the environment, high water solubility,and low affinity for organic matter. These properties contributeto their widespread presence in environmental waters and wastewaters,as well as drinking waters. The environmental concerns associatedwith USC-PFAS include their persistence, potential for groundwatercontamination, and challenges in remediation due to their resistance to conventional treatment methods.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_2_093d51b263.jpg" alt="EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources: Figure 1: Illustrative picture" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_2_093d51b263.jpg 245w," sizes="100vw" width="602" height="247"><h4>Occurence, uses and sources</h4>Ultrashort chain PFAS have been poorly characterised compared with their longer chain counterparts, and understanding of their intended and unintended transformation processes is limited. Sources of USCPFAS include degradation of precursor compounds, atmospheric degradation of hydrofluorocarbons and hydrochlorofluorocarbons used as refrigerants, and intentional direct use in products like batteries. Crucially, TFA, a major ultrashort-chain PFAS, is also formed through the environmental breakdown of fluorinated pesticides and pharmaceuticals. They are also byproducts from historical electrochemical fluorination (ECF) manufacture of longer chain PFAS. USC-PFAS are also found in urban and industrial waste, as well as aqueous film-forming foams (AFFFs) used in firefighting.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_5698c634d6.jpg" alt="EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources: Figure 2: PFSA and PFCA categorization based on carbon chain length – ultrashort-chain, short-chain and long-chain." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_5698c634d6.jpg 245w," sizes="100vw" width="1496" height="629"><h4>Fate and transport</h4>Carbon chain length plays a key role in determining the physicochemical characteristics of different PFAS chemicals. Long chain PFAS for instance are more hydrophobic, a property that has been leveraged by removal techniques using carbonaceous materials like granular activated carbon (GAC). By contrast, short and ultrashort chain PFAS are hydrophilic, with characteristically low pKa values (i.e. more acidic), high water solubilities and decreased log Koc values (i.e. less adsorptive to organic carbon). These attributes mean that USC-PFAS dissociate freely in aqueous environments and are less prone to sorption onto natural solids. The outcome is that USCPFAS are highly mobile in the aquatic environment and can travel far from contamination source areas.<h4>The global regulatory landscape</h4>Awareness about the persistence and bioaccumulative nature of C8-C14 PFAS began to attract widespread concern near the turn of the millennium, and inclusion of PFOS, PFOA and PFHxS on the persistent organic pollutants (POPs) list of the Stockholm Convention soon ensued. Consequentially, manufacturers began adopting short and ultrashort chain alternatives.However, with the change from C8 to C4 and shorter fluorinated carbon chains came the sacrifice to lower technical performance, and greater quantities were needed for equivalent performance. Hence, while it is generally accepted that short and ultrashort chain PFAS have lower toxicological profiles, environmental risks are not necessarily obviated since USC-PFAS presence, and continuous, cumulative, release suggests that adverse risks may lie ahead.Global regulations of USC-PFAS vary by region and are often included under broader PFAS regulations. For example, the European Union has specific guidelines and limits for the total concentration of PFAS compounds in drinking water (as set out in EU Directive 2020/2184). The EU's Drinking Water Directive established a limit of 0.5 μg/L for 'PFAS Total,' although ongoing discussions concern the specific methodology for incorporating trifluoroacetic acid (TFA). Furthermore, TFA was recently added to the priority list for the initial proposed sum of 24 PFAS in surface water. <a target="_blank" rel="noopener noreferrer" href="https://www.consilium.europa.eu/en/press/press-releases/2025/09/23/water-pollution-council-and-parliament-reach-provisional-deal-to-update-priority-substances-in-surface-and-ground-waters/">LINK</a><h4>PFAS sampling containers</h4>Only tested and verified sampling containers are recommended to ensure reliable results in PFAS analysis; these containers are available upon request from ALS laboratories.<h4>Targeted analytical services are becoming more accessible</h4>Testing for USC-PFAS is challenging due to several analytical factors. Background contamination and matrix noise can interfere with the detection of these compounds, particularly trifluoroacetic acid (TFA). Furthermore, sample preparation presents a significant challenge because of the constant risk of cross-contamination. To mitigate this risk, the sample preparation procedure was specifically designed to be as simple and straightforward as possible.Other USC-PFAS compounds generally have lower ambient background concentrations, which makes achieving lower Limits of Reporting (LOR) more readily achievable. ALS has successfully developed a testing method that includes USC-PFAS compounds in environmental water matrices, achieving a total LOR of 50 ng/L for TFA and even lower LORs for other USC compounds. The full list of analyzed USC-PFAS is summarized in table 1:<h5>USC-PFAS Compounds / CAS No.</h5><ul><li>Trifluoroacetic acid (TFA), Perfluoroethanoic acid (PFEtA): 76-05-1</li><li>Trifluoromethane sulfonic acid (TFMS), Perfluoromethane sulfonic acid (PFMeS): 1493-13-6</li><li>Perfluoroethane sulfonic acid (PFEtS): 354-88-1</li><li>Perfluoropropanoic acid (PFPrA): 422-64-0</li><li>Perfluoropropane sulfonic acid (PFPrS): 423-41-6</li><li>Trifluoromethane sulfinic acid: 34642-42-7</li><li>Lithium bis(trifluoromethanesulfonyl)imide (LiTFSI): 90076-65-6</li></ul>&nbsp;

EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources

<h4>Why Consider HILIC in Modern Oligonucleotide Analysis?</h4>Oligonucleotides play a central role in gene therapies, molecular diagnostics, and cutting-edge biomedical research. Before these molecules reach application, however, they must be carefully characterized to confirm purity, identity, and performance.Analyzing therapeutic oligonucleotides is far from straightforward. Their polarity, structural diversity, and modifications demand highly selective and MS-compatible separation methods. Although ion-pair reversed-phase LC (IP-RP-LC) remains the established standard for LC-MS workflows, hydrophilic interaction liquid chromatography (HILIC) is rapidly emerging as a powerful complementary technique.When IP-RP approaches reach practical or performance limits, HILIC offers an attractive alternative, delivering strong retention and efficient separation of highly polar oligonucleotides — without relying on ion-pairing reagents.<h4>How Does HILIC Separate Oligonucleotides?</h4>HILIC essentially reverses conventional reversed-phase logic. It combines:<ul><li>A polar stationary phase</li><li>An organic-rich mobile phase</li></ul>In this configuration, the stationary phase retains a thin, water-enriched layer on its surface. Polar analytes — such as oligonucleotides — partition into this aqueous layer while the acetonitrile-rich mobile phase passes through.This mechanism allows:<ul><li>Retention of short or highly polar oligonucleotides</li><li>Separation of modified sequences</li><li>LC-MS compatibility without ion-pairing reagents</li></ul>The key to performance lies in carefully tuning the parameters that govern HILIC selectivity and retention.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Key_Parameter_of_HILIC_Mode_fb364c9a8b.webp" alt="KNAUER: Figure 1 - Key Parameter of HILIC Mode." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Key_Parameter_of_HILIC_Mode_fb364c9a8b.webp 245w," sizes="100vw" width="945" height="433"><h4>What Controls Retention in HILIC?</h4>Several interaction mechanisms contribute to analyte behavior in HILIC:<h5>Hydrophilic Partitioning – The Primary Mechanism</h5>Polar molecules distribute into the thin water layer formed on the stationary phase surface. The greater the polarity of the analyte, the stronger its affinity for this layer, leading to increased retention.<h5>Hydrogen Bonding – Fine-Tuning Effects</h5>Functional groups such as –OH, –NH₂, and –COOH can form hydrogen bonds with the stationary phase, subtly influencing retention time and selectivity.<h5>Electrostatic Contributions – The Role of Charge</h5>Some HILIC materials (e.g., bare silica) exhibit surface charges. Depending on mobile phase pH and buffer composition, electrostatic interactions can either enhance or suppress analyte retention.<h5>Dipole and Ion–Dipole Interactions – Added Selectivity</h5>Highly polar or zwitterionic compounds — including phosphorylated species and sugars — may engage in dipole–dipole or ion–dipole interactions, further refining separation behavior.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hydrophilic_Partitioning_on_Water_enriched_Layer_41ab9ac902.webp" alt="KNAUER: Figure 2 - Hydrophilic Partitioning on Water-enriched Layer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hydrophilic_Partitioning_on_Water_enriched_Layer_41ab9ac902.webp 245w," sizes="100vw" width="945" height="409"><h4>How to Develop a HILIC Method for Oligonucleotides</h4>Like any chromatographic method, successful HILIC development follows a structured approach:<h5>Step 1: Define the Analytes</h5>Understand oligonucleotide length, polarity, charge, and modifications.<h5>Step 2: Select a Suitable Column</h5>Choose a polar stationary phase (e.g., amide or zwitterionic) appropriate for the oligonucleotide’s chemical properties.<h5>Step 3: Optimize the Mobile Phase</h5><ul><li>Start with 70–95% acetonitrile</li><li>Use a volatile buffer (e.g., ammonium formate or ammonium acetate, typically 5–20 mM)</li><li>Adjust pH (commonly between 6–8, with testing up to pH 9 if column stability allows)</li></ul>Small pH shifts can significantly alter selectivity and MS response.<h5>Step 4: Design the Gradient</h5>Employ a gradient that increases water content to modulate retention and separate oligonucleotides by size or chemical modification.<h5>Step 5: Match Injection Conditions</h5>Samples should be injected in a solvent similar to the starting mobile phase (high organic content) to prevent peak distortion.<h5>Step 6: Evaluate Performance</h5>Assess:<ul><li>Resolution</li><li>Retention time stability</li><li>Peak shape</li><li>Reproducibility</li></ul>Balancing separation performance with MS ionization efficiency is essential.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_HILIC_Method_Step_Procedure_for_Oligos_f3b9ea6b08.webp" alt="KNAUER: Table 1 - HILIC Method Step Procedure for Oligos." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_HILIC_Method_Step_Procedure_for_Oligos_f3b9ea6b08.webp 245w," sizes="100vw" width="945" height="357"><h4>Practical Tips for High-Performance HILIC</h4><h5>Maintain a Stable Water Layer</h5>A minimum of ~5% water is necessary at the start of the run. Too little water can eliminate retention entirely. Adjust water content carefully to fine-tune retention.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Water_Adjustment_Principle_for_HILIC_c0d8616f20.webp" alt="KNAUER: Figure 3 - Water Adjustment Principle for HILIC." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Water_Adjustment_Principle_for_HILIC_c0d8616f20.webp 245w," sizes="100vw" width="951" height="374"><h5>Use High Organic Content</h5>HILIC typically operates best with 70–95% acetonitrile. Polar analytes are retained more effectively under these conditions.<h5>Include a Volatile Buffer</h5>Use low concentrations (5–20 mM) of ammonium formate or acetate to:<ul><li>Stabilize pH</li><li>Reduce peak tailing</li><li>Maintain MS compatibility</li></ul><h5>Control Injection Solvent</h5>Avoid high-water sample solvents. They disrupt the partitioning mechanism and distort peaks.<h5>Allow Proper Column Equilibration</h5>HILIC columns require longer equilibration times than reversed-phase columns — typically 10–20 column volumes after major gradient or solvent changes.<h5>Consider Temperature</h5>Operating at 30–50 °C can:<ul><li>Improve peak shape</li><li>Reduce mobile phase viscosity</li><li>Stabilize retention behavior</li></ul><h4>From Alternative to Essential? Final Perspective</h4>HILIC-MS is steadily advancing as a strong, ion-pair-free option for oligonucleotide analysis. While IP-RP-LC still dominates in sensitivity for many applications, HILIC is closing the gap and offering valuable flexibility — particularly for highly polar or challenging sequences.Although HILIC follows different chromatographic principles than reversed-phase separations, once optimized, it can deliver robust and highly selective results.Rather than being a niche method, HILIC is becoming a strategic tool in modern oligonucleotide workflows — especially when MS compatibility and method versatility are priorities.If you are developing or refining an oligonucleotide LC-MS method, HILIC may be worth integrating into your analytical toolbox.<blockquote>For additional information or discussion, feel free to contact: <a>sales@knauer.net<svg><use></use></svg></a>For direct scientific inquiries, reach out to the author at: <a>kindler@knauer.net<svg><use></use></svg></a></blockquote>Stay tuned for more insights in the “Oligos Made Easy” series.

Oligos Made Easy - Part 4: HILIC

News from LabRulezLCMS Library - Week 09, 2026

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For more than 40 years, ALS has provided testing and technical solutions to clients all over the world in a wide range of industries. We have built our global reputation by embracing the latest technologies and innovative methodologies to deliver high quality testing services to clients. ALS is able to leverage a multifaceted international team while remaining agile enough to provide local expertise and personalized solutions to every one of our clients.

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Mettler-Toledo

Agilent Technologies

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Restek Corporation

SelectScience

Wiley

Waters Corporation

<h4>Live Interactive Web Series</h4>
We’ll work through a topic of the week (roughly 60 minutes) and discuss any questions that come up along the way. During these sessions, we’ll talk about how to care for your SEC-MALS or FFF-MALS system, help you brush up on your ASTRA and DYNAMICS data processing, discuss measuring dn/dc values and why dn/dc is important, and see how stable RI baselines are achievable.
Working with polydisperse samples? We’ll see how to leverage ASTRA’s distribution analysis and results fitting features. Special topics on error analysis, batch MALS work, and more.
The live interactive web series are 100% live sessions, with anonymous Q&amp;A welcome during and after the discussion. Free for all to attend.
<ul>
<li>Part 1 of 6: Why we love Multi-Angle Light Scattering! How MALS works and what it measures. Modes of operation and when to use them*
<ul>
<li>MALS theory and operating principles</li>
<li>Batch MALS vs. flow mode MALS: when and why</li>
<li>What MALS can and cannot measure directly</li>
</ul>
</li>
<li>Part 2 of 6: Getting to know your MALS hardware, including ‘proper care &amp; feeding’ of the system, practical guides for optimizing data quality, and how to know when your DAWN hardware needs special attention*
<ul>
<li>Understanding the MALS hardware internal and external components</li>
<li>Diagnosing hardware issues</li>
<li>How to help your MALS operate at its best</li>
</ul>
</li>
<li>Part 3 of 6: Let it flow - Leveraging MALS with Size Exclusion Chromatography SEC / Gel Permeation Chromatography GPC. Considerations for successful SEC-MALS / GPC-MALS operation. How to process ASTRA data on and after day 1*
<ul>
<li>Practical considerations for MALS in SEC/GPC mode</li>
<li>Understanding system settings &amp; coefficients such as inter-detector band broadening and normalization</li>
<li>Squeezing the most information from your SEC-MALS data</li>
</ul>
</li>
<li>Part 4 of 6: Advanced topics in ASTRA data analysis - uncertainty calculations, MW averages, mass recovery, distribution analysis, results fitting, and data exporting*
<ul>
<li>Understanding the ways ASTRA tracks and calculates system errors</li>
<li>Leveraging peak integration to understand what’s in your sample</li>
<li>When and why to use the various MW averages: Mn, Mw, Mz, Mp, Mavg, Mv, etc.</li>
<li>Efficient and easy ways to export exactly the data and results you want, right out of ASTRA</li>
</ul>
</li>
<li>Part 5 of 6: All about dn/dc values and concentration detectors (UV and RI) for MALS*
<ul>
<li>Understanding dn/dc, what it is, and how it is used with MALS</li>
<li>Measuring / determining dn/dc for your samples</li>
<li>UV detectors and their role in MALS</li>
<li>Mass recovery in ASTRA</li>
</ul>
</li>
<li>Part 6 of 6: MALS flow cell care: diagnosing and cleaning your flow cell*
<ul>
<li>How to avoid the need to clean your MALS flow cell in the first place</li>
<li>How to diagnose a dirty MALS flow cell</li>
<li>How to take apart and clean your MALS flow cell</li>
</ul>
</li>
</ul>
*It is recommended but not required that you attend all the sessions in this series.
<blockquote>
Presenter: Dr. Andy Meyer
</blockquote>
<blockquote>
Dr. Andy Meyer joined Wyatt Technology in 2001, serving for several years as an Applications Scientist, then as Dean of Wyatt&#39;s &quot;Light Scattering University&quot; and as Director of Customer Service &amp; Support. After managing multiple regions in North America, Dr. Meyer is now a Senior Application Scientist for Wyatt. As a Wyatt customer, he completed his Ph.D. in Applied Science – Polymer Chemistry from the College of William and Mary in Virginia.
</blockquote>

Waters Corporation: MALS flow cell care: diagnosing and cleaning your flow cell

MALS flow cell care: diagnosing and cleaning your flow cell

MALS flow cell care: diagnosing and cleaning your flow cell.

Dr. Wanqiu Zhang will report on the novel spatial multi-omics pipeline for integrating spatial lipidomics and transcriptomics data of prostate cancer.
Dr. Maria Mantas will report on quantitative mass spectrometry imaging of lipids in brain tissue using a multi class internal standard mixture.
<blockquote>
Presenter: Wanqiu Zhang (Machine Learning Scientist, Aspect Analytics)
</blockquote>
<blockquote>
Dr. Wanqiu Zhang obtained her PhD in the department of Electrical Engineering at KU Leuven (Belgium), specializing in machine learning, spatial multi-omics data analysis. She has joined Aspect Analytics as a machine learning scientist since she finished her PhD. She is currently actively leading and involved in multiple spatial multi-omics projects.
</blockquote>
<blockquote>
Presenter: Maria Mantas (Bioinformatician, Aspect Analytics)
</blockquote>
<blockquote>
Maria Mantas obtained her PhD in Computational Comparative Genomics at the University of Edinburgh, specialising in molecular biology, genetics, phylogenetics and pathway analysis using Big Data. Since joining Aspect Analytics as a Bioinformatician in 2022, Dr. Mantas has been involved in various spatial multi-omics integration studies, involving proteomics, lipidomics and metabolomics data, as well as spatial transcriptomics and imaging mass cytometry. Many of her current projects also include integrations with the Allen Mouse Brain Atlas.
</blockquote>

New Frontiers in Spatial Omics

Join us for the next webinar in the series, hosted by the US HUPO MS Imaging SIG - New Frontiers in Spatial Omics

In this webinar, our expert speakers will provide an overview of the fundamentals of light scattering for macromolecular characterization by size exclusion chromatography (SEC), including good practice for sample preparation and experimental methods.
The use of multi-angle light scattering (MALS) as a tool to determine effective SEC conditions for the macromolecule of interest will be discussed. A description of the measurable quantities SEC-MALS offers and the careful considerations that are required in fitting light scattering data to ensure accurate macromolecular molecular weight and size measurements will be provided.
Key learning objectives
<ul>
<li>Explore the fundamentals of light scattering and absolute molecular weight detection for macromolecules</li>
<li>Learn about sample preparation, instrumentation setup, dn/dc requirements, and good practices</li>
<li>Understand what is needed for fitting light scattering data to ensure quality data for molecular weight and size</li>
<li>Explore SEC-MALS applications</li>
</ul>
Who should attend?
<ul>
<li>Principal investigators</li>
<li>Laboratory technicians/technologists</li>
<li>Synthetic polymer scientists</li>
<li>Biopolymer scientists</li>
<li>Analytical scientists</li>
</ul>
<h4>View other webinars in this series here:</h4>
Part 1: <a href="https://lcms.labrulez.com/webinar/3690">Unraveling the Intricacies of Size Exclusion Chromatography – Strength and Limitations</a>
Part 2: <a href="https://lcms.labrulez.com/webinar/3691">Detection in Gel Permeation Chromatography /Size Exclusion Chromatography</a>
Part 4: <a href="https://lcms.labrulez.com/webinar/3693">Concepts, Challenges and Applications for 2D-LC of Polymers</a>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Dr. Paul Balding (Associate Research Scientist, Dow Inc.)
</blockquote>
<blockquote>
Dr. Paul Balding received his PhD in Chemistry from The Georgia Institute of Technology for work on the synthesis and characterization of model polyelectrolytes and dynamics behavior in low salt solutions. He received a BSc Honours in Chemistry from the University of Wollongong, Australia, working on the rheology and mechanical performance of 3D-printed hydrogel materials. He is currently a subject matter expert working in the separations science team at Dow Chemical in the polyolefin space with a focus on high-temperature SEC-coupled MALS.
</blockquote>
<blockquote>
Presenter: Dr. Daniela Held (R&amp;D Director, GPC/SEC Center of Excellence, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Dr. Daniela Held received her PhD in Chemistry from the University of Mainz, Germany, for work on the synthesis and characterization of star-branched polyelectolytes. In 2000, she joined the software team of PSS Polymer Standards Service GmbH, developing workflow solutions for advanced detection techniques and developing customer trainings. Through the acquisition of PSS by Agilent Technologies, she became R&amp;D director at the GPC/SEC Center of Excellence in Mainz, Germany.
</blockquote>
<blockquote>
Presenter: Jemima Arnold (Editorial Assistant, SelectScience)
</blockquote>
<blockquote>
Jemima studied Cellular and Molecular Medicine at the University of Bristol, UK, and as a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

SelectScience: Characterization of Macromolecules by SEC-Light Scattering

Characterization of Macromolecules by SEC-Light Scattering

An overview of the fundamentals of light scattering for macromolecular characterization by size exclusion chromatography (SEC).

<a href="https://gcms.labrulez.com/products/1016">Wiley’s KnowItAll software</a> offers integrated solutions to identify, analyze, and manage analytical and chemical data. Combined with comprehensive high-quality spectral reference databases, it provides one of the most advanced technologies available for fast, reliable spectral analysis.
<a href="https://gcms.labrulez.com/products/1016">KnowItAll</a> supports multiple techniques and instrument vendor formats. So whether you use one or more techniques, it can work across a single lab using one instrument or multiple labs with multiple instrument types. Using a vendor neutral solution like KnowItAll is important further in that it future proofs your lab, so even if you add instruments or change vendors your analytical workflow stays intact. 
In this session, we will demonstrate through a series of live examples some of the powerful spectral search tools available within the KnowItAll for unknown spectral analysis in a multi-technique laboratory setting. 
<h5>You will discover:</h5>
<ul>
<li>How KnowItAll offers a consolidated workflow for one or more analytical techniques by combining all the tools needed for spectral analysis and data management into a single, easy-to-use interface. </li>
<li>How to perform an adaptive search to find similar compounds where a fragment group can be present or absent compared to the unknown. </li>
<li>How to perform fast orthogonal searches for unknown spectra within the KnowItAll software and interpret those results. </li>
<li>In addition to our empirical libraries, you will discover how to use a new predicted library to expand the addressable chemical space for spectral identification.</li>
</ul>
This session is recommended for:
<ul>
<li>Scientists and chemists who use multiple laboratory techniques or are interested in learning more about our capabilities. </li>
<li>Professors and students engaged in chemistry and multiple laboratory technique courses or lab work.</li>
</ul>
<blockquote>
Presenter: Dr. Leanna Ergin (Assistant Editor, Wiley Science Solutions)
</blockquote>

Wiley: Introduction to KnowItAll for Multi-Spectral Techniques

Introduction to KnowItAll for Multi-Spectral Techniques

We will demonstrate some of the powerful spectral search tools available within the KnowItAll for unknown spectral analysis in a multi-technique laboratory

Join us for this exclusive webinar as we introduce the latest version of our OpenLab software.
Prepare to revolutionize your laboratory experience with unparalleled precision, fortified security, and tailored solutions for the dynamic laboratory world. Our software ensures seamless migration from legacy systems, empowering you to embrace the future with confidence. Experience enhanced performance and unwavering resilience in every operation.
Highlights:
<ul>
<li>Live Demo to show the latest advancements in OpenLab 2.8</li>
<li>Why OpenLab 2.8 matters for your biopharma workflows</li>
<li>Why OpenLab 2.8 matters for your data integrity</li>
<li>Why OpenLab 2.8 matters for your energy and chemicals analysis</li>
<li>Q&amp;A session – Because your questions matter</li>
</ul>
Register now and join us on this exciting journey!
<blockquote>
Presenter: Kevin Kölliker (Lab Informatics Application Engineer, Agilent Technologies)
</blockquote>
<blockquote>
Kevin Kölliker is a Lab Informatics Application Engineer for Agilent Technologies.
</blockquote>
<blockquote>
He studied Chemistry and Business informatics, and he worked as a Lab Technician, and as a Field Service Engineer before joining Agilent.
</blockquote>
<blockquote>
Combined he has more than 10 Years of Experience in the analytical laboratory and therefore met already multiple different workflows. He works together with the Lab Informatics Product Specialists to demonstrate the Power of OpenLab, and to customize it to meet the needs of our Customers.
</blockquote>
<blockquote>
Presenter: Frederick Thibaut (Lab Informatics Application Engineer, Agilent Technologies)
</blockquote>
<blockquote>
Frederick Thibaut is a Lab Informatics Application Engineer for Agilent Technologies.
</blockquote>
<blockquote>
He holds a master in engineering from KU Leuven and informatics degree from the university of Michigan.
</blockquote>
<blockquote>
Before joining Agilent as an Enterprise Project Manager and Application Engineer he was working as an Informatics consultant.
</blockquote>

Agilent: Unveiling a Connection That Matters - OpenLab 2.8

Unveiling a Connection That Matters - OpenLab 2.8

Join us for this exclusive webinar as we introduce the latest version of our OpenLab software.

Reference standards must perform at a consistent level each and every time you use them. Variations in analyte concentration can disrupt analyses, impede your lab’s workflow, and lead to wasted time and money.
In order to create trust in the use of reference standards worldwide quality standards have been established through which the production of reference standards is regulated by numerous guidelines. The webinar gives insights how Restek as a manufacturer of certified reference materials meets these requirements. In total, there are 10 decisive steps that determine the quality of Restek&#39;s reference materials.
<ul>
<li>Learn what certified reference materials (CRMs) are, and why you need them.</li>
<li>Explore the quality controls needed to consistently ensure accurate reference standards.</li>
<li>Review what information is available in certificates and data packs</li>
</ul>

Restek Corporation: Build a Rock-Solid Analytical Foundation with Restek Certified Reference Standards

Build a Rock-Solid Analytical Foundation with Restek Certified Reference Standards

The webinar gives insights how Restek as a manufacturer of certified reference materials meets numerous guidelines and requirements.

Restek Certified Reference Standards

The recent pandemic has forced the field of medicine to witness groundbreaking advancements with the development and widespread adoption of novel therapeutic modalities, particularly concerning nucleic acid-based medicines. Among these, messenger RNA (mRNA), lipid nanoparticle (LNP) delivery systems, and viral vector-based medicines have emerged as transformative technologies, unlocking new possibilities for treating a myriad of genetic diseases and cancers. As such, ensuring the quality and integrity of these therapeutics becomes paramount.
Impurities and structural integrity issues can impact the safety and efficacy of these medicines. Herein lies the emerging significance of size exclusion chromatography (SEC) combined with MALS for impurity and integrity analysis. In this webinar, we will highlight the critical quality attributes using new 1000 Å ultrawide pore columns with MALS detection to resolve and analyze long-chain nucleic acids (RNA/DNA), lipid nanoparticles (LNPs) and viral vector formulations.
<ul>
<li>Development of high-resolution and robust multiattribute methods for mRNA integrity and aggregate analysis</li>
<li>Method conditions for Intact Lipid Nanoparticle (LNP) heterogeneity analysis</li>
<li>Aggregate analysis and elucidating degradation pathways with high efficiency and low adsorption hardware using the new 1000 Å ultrawide pore columns</li>
</ul>
<blockquote>
Presenter: Dr. Adam Crowe (Sr. Manager Analytical Development, Precision NanoSystems ULC - part of Cytiva)
</blockquote>
<blockquote>
Adam Crowe manages a mult-discipline team at Precision NanoSystems ULC (part of Cytiva) tasked with developing novel analytical assays related to lipid nanoparticles (LNP) and nanomaterials for drug delivery. Notably, Adam and his team have increasingly leveraged cutting-edge technologies, such as LC-MS approaches, to tackle complexities of LNP characterization. Latest studies were focused on the detail characterization of impurities found in ionizable liquids.
</blockquote>
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Presenter: Lavelay Kizekai (Principal Scientist, Waters Corporation, Milford, Massachusetts)
</blockquote>
<blockquote>
Lavelay Kizekai is a Principal Scientist at Waters Corporation. He joined Waters in 2009 and has since applied himself to developing new LC methods, leading column technology product development work, and establishing insight-based QC procedures for Waters products. His primary area of focus is biomolecule separations using Size-Exclusion Chromatography (SEC). He was a core member of the team that recently bought to market a column suited to platform methods for monoclonal antibodies and antibody drug conjugates. He is currently leveraging these SEC technologies and even newer breakthroughs for the characterization of gene-based therapeutics by multi-angle light scattering (SEC-MALS).
</blockquote>
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Presenter: Mandana Fasth (Principal Product Manager, Waters Corporation)
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<blockquote>
Mandana Fasth is the Global Principal Product Manager at Waters Corporation. She received her Master degree in Biochemistry from Uppsala university in Sweden. With more than 20 years&#39; experience working with biologics workflows such as Sized-Exclusion Chromatography and column development projects, she is a well versed at training and supporting scientists with method development for characterization and purification of biologics.
</blockquote>

Waters Corporation: Unveiling CQA Insights for mRNA, LNPs and Viral Vectors by SEC and MALS with Novel 1000 Å Ultrawide Pore Particle Columns

Unveiling CQA Insights for mRNA, LNPs and Viral Vectors by SEC and MALS with Novel 1000 Å Ultrawide Pore Particle Columns

This webinar will highlight the critical quality attributes using new 1000 Å ultrawide pore columns with MALS detection.

CQA Insights for mRNA, LNPs and Viral Vectors by SEC/MALS

Join us LIVE for the Waters Journal Club.
This interactive session will bring together the MS community to discuss some of the most popular and exciting peer-reviewed publications in a friendly and open forum. 
Ahead of each meeting, we will send out a link to the discussion article, then during the session an expert will summarise the main findings and answer your questions. 
Be prepared make sure you <a href="https://pubs.acs.org/doi/10.1021/acs.analchem.2c02406">read Advancing Cyclic Ion Mobility Mass Spectrometry Methods for Studying Biomolecules: Toward the Conformational Dynamics of Mega Dalton Protein Aggregates</a>
We&#39;re going to be joined by ETH Zurich, one of the world&#39;s leading universities in science and technology, known for its cutting-edge research and innovation, pioneering effective solutions to the global challenges of today and tomorrow.
This paper describes how Waters&#39; SELECT SERIES Cyclic IMS can be utilized for the analysis of large biomolecular systems, including temperature-induced protein aggregates of masses greater than 1.5 MDa, as well as a 63 kDa oligonucleotide complex.
Reasons to Attend:
<ul>
<li>Debate some of the most topical papers from all-time favourites to some of the latest releases</li>
<li>Focus on understanding the benefit of Cyclic IMS and how to get the most out of the system for native mass spectrometry and biomolecule analysis</li>
<li>Gain a greater understanding of applications and technologies by asking questions of the authors and/or expert panel</li>
<li>No pre-recorded content, authors will be LIVE and on camera to answer questions</li>
</ul>
Abstract: Native mass spectrometry is a powerful tool for the analysis of noncovalent complexes. When coupled with high-resolution ion mobility, this technique can be used to investigate the conformational changes induced in said complexes by different solution or gas-phase conditions. In this study, we describe how a new-generation high-resolution ion mobility instrument equipped with a cyclic ion mobility cell can be utilized for the analysis of large biomolecular systems, including temperature-induced protein aggregates of masses greater than 1.5 MDa, as well as a 63 kDa oligonucleotide complex. The effects of and the interplay between the voltages applied to the different components of the cyclic ion mobility spectrometry system on ion transmission and arrival time distribution were demonstrated using biomolecules covering the m/z range 2000–10,000. These data were used to establish a theoretical framework for achieving the best separation in the cyclic ion mobility system. Finally, the cyclic ion mobility mass spectrometer was coupled with a temperature-controlled electrospray ionization source to investigate high-mass protein aggregation. This analysis showed that it was possible to continuously monitor the change in abundance for several conformations of MDa aggregates with increasing temperature. This work significantly increases the range of biomolecules that can be analyzed by both cyclic ion mobility and temperature-controlled electrospray ionization mass spectrometry, providing new possibilities for high-resolution ion mobility analysis.
<blockquote>
Presenter: Dr. Julian Harrison (Staff of Professorship for Analytical Chemistry, ETH Zurich)
</blockquote>
<blockquote>
Julian Harrison received his Ph.D. in Chemistry from the University of Wollongong in 2020, working under the supervision of Prof. Jennifer L. Beck and Dr. Celine Kelso. In 2021, he was employed as a postdoctoral researcher at ETH Zürich under the direction of Prof. Renato Zenobi. ]
</blockquote>
<blockquote>
He works in multiple fields, primarily studying protein complexes, enzymes, snake venoms, and new therapeutics using native, temperature-controlled, and ion mobility mass spectrometry. In collaboration with Adam Pruška, he is also helping design a new temperature-controlled source, which will be the basis for their new company.
</blockquote>
<blockquote>
Presenter: Dr . Adam Pruška (Staff of Professorship for Analytical Chemistry, ETH Zurich)
</blockquote>
<blockquote>
Adam Pruška studied analytical chemistry at Masaryk University in the Czech Republic and Justus-Liebig University in Germany under the supervision of Prof. Jan Preisler and Prof. Bernhard Spengler.
</blockquote>
<blockquote>
He finished his M.Sc. in 2017, specializing in matrix-assisted laser desorption/ionization (MALDI) and the multimodal bioimaging of tumor spheroids. Continuing his academic career, Adam obtained his Ph.D. at ETH Zurich in 2023, within the group of Prof. Renato Zenobi.
</blockquote>
<blockquote>
His doctoral research focused on exploring the thermodynamics of nucleic acids, proteins, and their non-covalent complexes. He is embarking on an entrepreneurial path as a postdoctoral researcher, developing temperature-controlled electrospray ionization technology for commercial use.
</blockquote>
<blockquote>
Presenter: Philipp Bittner (Student / Programme Doctorate at D-​CHAB, ETH Zurich)
</blockquote>
<blockquote>
Philipp Bittner graduated from the University of Regensburg, Germany, with a B.Sc. in Biology (2015) and an M.Sc. in Biochemistry (2018).
</blockquote>
<blockquote>
After finishing his first research project about photoresponsive enzyme complexes in Prof. Reinhard Sterner&#39;s group, he joined Prof. Renato Zenobi&#39;s native MS team for his Ph.D. thesis (2020).
</blockquote>
<blockquote>
His research now focuses on mass spectrometric investigations of modified collagen peptides, DNA-encoded chemical libraries, and antibody glycosylation patterns.
</blockquote>
<blockquote>
Presenters: Despoina Svingou (Student / Programme Doctorate at D-CHAB, ETH Zurich)
</blockquote>
<blockquote>
Despoina Svingou obtained her B.Sc. in Chemistry and M.Sc. in Natural Products Chemistry &amp; Pharmacognosy from the National and Kapodistrian University of Athens. Her research focused on developing LC-MS methods for the analytical determination of bioactive compounds in various complex matrices. Currently, she is pursuing a Ph.D. in Analytical Chemistry at ETH Zurich under the supervision of Prof. Renato Zenobi.
</blockquote>
<blockquote>
She utilizes cutting-edge techniques such as native mass spectrometry and gas-phase fluorescence spectroscopy to unravel the molecular mechanisms underlying neurodegenerative diseases and develop novel methodologies to characterize amyloidogenic proteins and their complexes.
</blockquote>
<blockquote>
Presenter: Dale Cooper-Shepherd (Principal Scientist, Waters Corporation, UK)
</blockquote>
<blockquote>
Dale Cooper-Shepherd undertook PhD studies at the University of Leeds and conducted postdoctoral research at the University of Oxford, UK. Throughout his training, Cooper-Shepherd specialized in applying MS techniques in the field of structural biology. He joined Waters in 2015 and since 2019 has been working on the SELECT SERIES of instruments.
</blockquote>

Waters: Waters Journal Club: Advancing Cyclic Ion Mobility Mass Spectrometry Methods for Studying Biomolecules

Waters Journal Club: Advancing Cyclic Ion Mobility Mass Spectrometry Methods for Studying Biomolecules

Waters' SELECT SERIES Cyclic IMS can be utilized for the analysis of large biomolecular systems.

Advancing Cyclic Ion Mobility Mass Spectrometry

Event Program
<ul>
<li>Loss of water/steam chemistry knowledge and expertise</li>
<li>Most common water/steam quality issues in cogen plants that lead to equipment damage</li>
<li>The ongoing transition from lab to on-line measurements and testing</li>
<li>The commonly overlooked risks of organic contamination to boiler health</li>
</ul>
Practical Tips, Solutions, and Resources to Help Your Cogeneration.
This educational webinar will review emerging trends and challenges in successfully managing water and steam quality that are facing cogeneration power and steam generation (CHP) units. 
Staff responsible for power and steam generation units located within today’s Chemical, Refining, Pulp &amp; Paper, Sugar, Fertilizer, Waste to Energy and other similar type of plants, are looking for ways to learn from others, evolve and improve operational control of their cogeneration processes. 
Valuable take-aways will include tips, solutions and additional resources to the challenges reviewed. Attendees will have an opportunity to ask the presenter questions about the topics covered or anything else related to cogeneration.
Individuals involved in water treatment, boiler and steam quality management, maintenance of water/steam analytics and ensuring operation uptime will find this training helpful.
<blockquote>
Presenter: Kirk Buecher (Director of Power Solutions, METTLER TOLEDO)
</blockquote>
<blockquote>
Kirk Buecher Director of Power Solutions, METTLER TOLEDO Thornton Kirk Buecher has more than 30 years of experience in precision measurement and analysis technologies. For the past 12 years, he has focused on analyzing the chemistry of water used in all types of power and steam generation, including nuclear, biofuel, geothermal, and cogeneration plants. More recently, he has expanded his experience into the requirements for successful green hydrogen generation.
</blockquote>

Mettler-Toledo: Cogen Water Steam Quality Management - Emerging Trends and Challenges

Cogen Water Steam Quality Management - Emerging Trends and Challenges

Emerging trends and challenges in successfully managing water and steam quality that are facing cogeneration power and steam generation (CHP) units.

Cogen Water Steam Quality Management

Based on The New ISO 8655 p. 10 for 2024
Event Program
<ul>
<li>Introduction and overview of what&#39;s new in the 2024 ISO 8655 part 10</li>
<li>Requirements and best practices</li>
<li>User qualification and re-qualification</li>
<li>POVA qualifitcation and requalification (routine testing, pass/fail rules, etc)</li>
<li>Solutions to fulfill requirements</li>
</ul>
In this live webinar, we will give you an overview of the ISO 8655 part 10, which was released in March 2024.
You will learn about updated requirements and how the new chapter can affect your lab. Ensure you are up to date with this new regulation while at the same time improving the quality of your results. 
We will also give you an overview of Rainin&#39;s solutions that help you comply with this new chapter of ISO 8655. As this is a webinar you will also have the chance to ask questions to our experts and discuss what aspects need to be considered in comp;iance with the new chapter. 
<blockquote>
Presenter: Irene Ni
</blockquote>
<blockquote>
Presenter: Martin Huber
</blockquote>
<blockquote>
Presenter: Fabian Troll
</blockquote>

Mettler-Toledo: User Guidance and Qualification in Pipetting

User Guidance and Qualification in Pipetting

In this live webinar, we will give you an overview of the ISO 8655 part 10, which was released in March 2024.