Per- and polyfluoroalkyl substances (PFAS) have become a major global concern due to their persistence, bioaccumulation, and potential health impacts. With thousands of known PFAS compounds, and new variants continuing to emerge, identifying and quantifying them across complex environmental and biological samples is an ongoing analytical challenge.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg" alt="Thermo Fisher Scientific: From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg 245w," sizes="100vw" width="1024" height="574"><h3>Why PFAS analysis matters</h3>PFAS are used in everything from firefighting foams to non-stick coatings, yet their chemical stability means they persist in the environment and accumulate in living organisms. Regulators and scientists around the world are racing to better understand their distribution, fate, and toxicity.But with tens of thousands of PFAS, many without available reference standards, traditional targeted analysis alone isn’t enough. Analysts increasingly rely on non-targeted analysis (NTA) workflows powered by high-resolution accurate-mass (HRAM) mass spectrometry and advanced software to uncover the full picture.<h3>Addressing the challenges of PFAS identification</h3>Successful non-targeted PFAS analysis requires overcoming several analytical hurdles:<ol><li>Acquiring high-quality MS¹ and MS² data with ultra-high mass resolution and accuracy.</li><li>Detecting low-abundance PFAS hidden within thousands of background compounds.</li><li>Leveraging comprehensive spectral libraries and databases for confident identification.</li><li>Applying data filtering and scoring tools to assign confidence levels to annotations.</li><li>Using visualization and statistical tools to interpret and compare complex datasets.</li></ol><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Thermo Scientific Compound Discoverer 3.5 software </a>brings together all the components needed for robust PFAS identification—integrating <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=22&amp;manufacturerItems=3">HRAM Orbitrap MS</a> data with an expanded suite of PFAS-specific libraries and databases.In a single workflow, users can:<ul><li>Detect and annotate compounds automatically from LC-MS data.</li><li>Determine possible elemental formulas using isotope pattern and accurate mass information.</li><li>Compare MS² spectra against <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1764">mzCloud</a>, NIST 2023, and in silico PFAS libraries.</li><li>Cross-reference annotations with the EPA PFAS Structure List and the NIST Suspect List of PFAS.</li><li>Use PFAS-specific fragment and neutral loss databases to strengthen identification confidence.</li></ul>With these resources combined, the software enables confident annotation of more than 40,000 PFAS compounds!<h3>New tools for confident, streamlined PFAS identification</h3>Compound Discoverer software introduces several new features designed to simplify and accelerate PFAS NTA workflows:<ul><li>Apply PFAS Scoring Node: Automatically calculates PFAS-specific parameters and a new PFAS Score—no R scripting required.</li><li>PFAS Plot: A visual tool that uses mass-defect metrics to highlight probable PFAS in complex datasets, following methods from Kaufman et al. (2022).</li><li>Expanded suite of libraries and databases: Combines mzCloud, NIST, in silico, PFAS fragment databases, and a new neutral loss database for greater confidence.</li><li>Advanced data visualization: Tools like Kendrick Mass Defect and Molecular Network plots reveal homologous PFAS series and related compound families.</li><li>Built-in statistical analysis tools: Compare PFAS composition between samples using Principal Component Analysis, Differential analysis and Hierarchical Cluster Analysis.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg" alt="Thermo Fisher Scientific From Complexity to Clarity Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg 245w," sizes="100vw" width="800" height="395"><h3>From complexity to clarity</h3>By uniting Orbitrap HRAM data with the most comprehensive PFAS libraries available, Compound Discoverer 3.5 software streamlines non-targeted PFAS analysis—helping laboratories move from data complexity to confident chemical clarity.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5612">Watch the on-demand webinar</a> to see the full workflow in action and learn how Compound Discoverer software can enhance your PFAS analysis.<ul><li>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#PFAS #CompoundDiscoverer #SpectralLibraries</li></ul><blockquote><h5>Zoe Julian</h5>Zoe Julian is a Product Marketing Manager for Chromatography and Mass Spectrometry software at Thermo Fisher Scientific. With over 12 years' experience of working with chromatography and mass spectrometry techniques with a background in GC and GC-MS(/MS) organic environmental analysis, she has helped many laboratories and industries improve their workflows through a unique understanding of their challenges. Transitioning to a focus on Chromeleon CDS she has shown customers how software solutions can improve their productivity and support their day-to-day operations.</blockquote>

From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software

<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

Software

Article

Product

Application

Academy

Video

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

New Food magazine

Separation Science

Metrohm

EMN Metabolomics Society

Agilent Technologies

Phenomenex

Wiley

SCIEX

Waters Corporation

*Restek has added a new tool to its suite of virtual chromatography software. Learn how the Pro EZLC Chromatogram Modeler can make column selection and optimization for LC-MS/MS methods for therapeutic and abuse drugs, nitrosamines, and PFAS compounds easier and faster.* 
Method developers and analysts will appreciate access to a virtual selection of columns to aid in choosing and optimizing methods. We will even cover adding novel psychoactive substances to an existing method without the need for an instrument.
By attending this presentation, you will:
<ul>
<li>*Learn about the Pro EZLC Chromatogram Modelling tool and how to access it.*
</li>
<li>Learn how to perform LC-MS/MS method development and optimization easier, faster, and without an instrument.
</li>
</ul>
<blockquote>
Presenter: Rock J. Vitale (Technical Director of Chemistry, Senior Principal at Environmental Standards, Inc.)
</blockquote>
<blockquote>
Paul brought 24 years of analytical and chromatography expertise to Restek, including 16 years performing LC-MS/MS. He earned his BS in biology and a minor in chemistry from the University of Pittsburgh, after which he was an environmental GC, GC-MS, and HPLC chemist for nearly a decade. Paul has also worked as a scientist and was principal investigator, group leader, technical leader, and method developer for a contract research organization providing LC-MS/MS support to the food safety, environmental, animal health, and crop protection industries. Paul was also a bioanalytical laboratory director, providing analytical laboratory services for drug development. As our liquid chromatography product manager, Paul&#39;s main focus is to grow and develop Restek&#39;s LC product lines and to provide our customers with high-performing solutions to their LC challenges.
</blockquote>

Separation Science: LC-MS/MS Method Development and Optimization with Restek’s Pro EZLC Chromatogram Modeler

LC-MS/MS Method Development and Optimization with Restek’s Pro EZLC Chromatogram Modeler

LC-MS/MS Method Development with Restek’s Pro EZLC

Increasing concern about PFAS compounds in food and other complex matrices has led to a greater need for reliable and accurate quantitation—something that has already been addressed in many publications.
Scientists are still investigating the full extent of the problem, however, and are discovering challenges that require a much broader scope of analysis. Full-spectral, highly sensitive, accurate mass spectrometry with the SCIEX ZenoTOF 7600 system provides additional information that can be used to identify new analytes and better define the sample matrices, in addition to a complete quantitative, confirmatory workflow. Using novel SWATH DIA, EAD and the Zeno trap for quan and qual workflows, researchers are now able to get a complete and detailed analysis of their samples. The comprehensive data aids in understanding the extent of the PFAS problem and defining how it can be alleviated for future generations.
In this webinar, you will hear about:
<ul>
<li>High-sensitivity workflows for screening and quantitation with precise confirmation, using the ZenoTOF 7600 system
</li>
<li>SWATH DIA for complete classification of complex samples
</li>
<li>The use of EAD to identify PFAS compounds in food matrices
</li>
</ul>
<blockquote>
Presenter: Dr. Jianru Stahl-Zeng (Senior Technical Marketing Manager EMEAI, SCIEX)
</blockquote>
<blockquote>
Jianru has been part of SCIEX since 2001. She was initially part of the application support team and covered both proteomics and small molecule applications. Jianru moved into technical marketing and focused on food and environmental applications. Since 2018, she has been managing the EMEA technical marketing team and projects. Jianru is also the Global Technical Leader for food and environmental applications.
</blockquote>

SCIEX: Accurate mass workflows for PFAS in food using the ZenoTOF 7600 system

Accurate mass workflows for PFAS in food using the ZenoTOF 7600 system

High-sensitivity workflows for screening and quantitation with precise confirmation, using the ZenoTOF 7600 system.

Accurate mass workflows for PFAS in food

Key Learning Points:
<ul>
<li>Explore modern chromatographic techniques for AAV quantification and characterization.
</li>
<li>Gain a comprehensive understanding of Critical Quality Attributes (CQAs) and their significance in drug development.
</li>
<li>Learn to navigate common CQA challenges at different development stages for meeting industry standards.
</li>
<li>Discover the importance of selecting tailored chemistries and method conditions to ensure robust, efficient, and precise analytical methods.
</li>
</ul>
Who Should Attend:
<ul>
<li>Any Analytical Development Scientists, QC Analysts / Managers, or R&amp;D Scientists who are looking to learn more about the role of chromatography in ensuring AAV quality and safety in drug development.</li>
</ul>
<blockquote>
Presenter: Shilin Lok Yee Cheung (Sr. Research Scientist, Phenomenex)
</blockquote>
<blockquote>
Dr. Cheung received her BS (2006) and Ph.D. (2012) in Bioanalytical Chemistry from the University of Toronto. She has developed, qualified, and validated innovative analytical methods in CE based microfluidics, automated low volume UV-vis spec., and UHPLC assays. Her methods have been applied to manufacturing process developments, formulation and stability and DS/DP characterization and specification testing. At the Phenomenex Innovation Campus she is focused on developing fit for purpose analytical methods to help advance developments in gene therapeutics. 
</blockquote>

Phenomenex: Critical Quality Attributes for Adeno-Associated Virus Analysis

Critical Quality Attributes for Adeno-Associated Virus Analysis

Quality Attributes for Adeno-Associated Virus Analysis

<h4>Event Overview</h4>
The shift towards biopharmaceuticals during the last decade posed some challenges in the construction of modern liquid chromatography systems, particularly addressing the problem of non-specific interactions, making the development of reproducible analytical methods a challenge.
This presentation covers and discusses the latest advances in liquid chromatography technology to minimize the effect of non-specific interactions. The discussion ranges from the basics of &#39;metal-sensitive&#39; molecules to the method development strategies for the analysis of biomolecules. We provide a comprehensive overview of the major analytical challenges that could be faced when an accurate analysis is required for &#39;metal-sensitive&#39; compounds, and we discuss the best countermeasures that can be adopted to ensure &#39;method robustness&#39;. Moreover, we describe efficient data processing solutions for the characterization of oligonucleotide products and impurities for LC-MS datasets.
By attending this presentation, you will learn key strategies for:
<ul>
<li>Advances in biomolecule analysis
</li>
<li>How you can improve data quality, sensitivity and resolution for metal-sensitive molecules (for small molecule and large biotherapeutic analysis)
</li>
<li>Advanced data processing technology for characterizing oligonucleotide products and impurities for LC-MS datasets
</li>
</ul>
<blockquote>
Presenter: Raymond Wong, MChem, Ph.D., MRSC (Senior Product Manager and National Sales Manager, Shimadzu UK Limited)
</blockquote>
<blockquote>
Raymond Wong has over 20 years’ experience working within the analytical, purification and pharmaceutical industry. Dr Wong started as a synthetic organic chemist where early projects included investigating new fluorinated compounds with Ineos Fluor for early OLED technology. He was later involved with synthesising modified oligonucleotides for antisense therapeutics, before transitioning to producing dynamic combinatorial libraries to investigate Sequencing by Synthesis (SbS). Following several diverse pharma campaigns within a contract laboratory, Raymond moved to Phenomenex to support method development across different sectors before joining Shimadzu over 8 years ago allowing him to collaborate with people looking to progress their research.
</blockquote>

Shimadzu UK Limited: Need more clarity with your chromatography? This will PEEK your interest.

Need more clarity with your chromatography? This will PEEK your interest

Need more clarity with your chromatography?

This webinar will explore the shift towards sustainable, animal-free protein ingredients, emphasizing their similarity to traditional sources in composition and functionality. We’ll showcase the transformative impact of the Thermo Scientific™ Prima PRO Process Mass Spectrometer in biofoods manufacturing, and how it has become a cornerstone technology in enhancing control, efficiency, and yields in fermentation processes.
Expect a session rich in expert perspectives, with Dan and Andrew sharing their thoughts on current trends, challenges, and future directions in fermentation monitoring and analysis. This webinar aims to address the broader issues in the biofoods industry, exemplified by real-life applications and case studies from Geltor’s journey.
Whether you’re a professional in the biofoods industry, a bioprocess engineer, or simply intrigued by the future of food technology, this webinar will provide you with valuable insights and a glimpse into the evolving landscape of biofood production.
What attendees will learn:
<ul>
<li>The advanced role of fermentation in modern biofood production.
</li>
<li>The impact of real-time MS monitoring in fermentation processes.
</li>
<li>Techniques to optimize manufacturing efficiency and maximize yields.
</li>
<li>Future trends and insights from a leading expert in the field.
</li>
</ul>
Whether you’re a professional in the biofoods industry, a bioprocess engineer, or simply intrigued by the future of food technology, this webinar will provide you with valuable insights and a glimpse into the evolving landscape of biofood production.
<blockquote>
Presenter: Dan Merriman (Senior Advisor, Marketing Process Monitoring, Thermo Fisher Scientific)
</blockquote>
<blockquote>
with his 35-year expertise in mass spectrometry and process analytics, will delve into the nuances of collagen fermentation, showcasing how Thermo Fisher’s MS technology propels the industry forward. His insights will unravel the complexities of this process and its pivotal role in sustainable food production.
</blockquote>
<blockquote>
Presenter: Andrew Saarni (Director of Fermentation at Geltor)
</blockquote>
<blockquote>
brings a practical perspective from the forefront of bio-designed protein production. His decade-long experience in bioprocess engineering and his instrumental role in advancing bacterial fermentation techniques are key to understanding Geltor’s success story. Andrew’s expertise in scaling sophisticated fermentation processes will offer a comprehensive view into the operational aspects of producing high-quality, ethical biofoods.
</blockquote>

New Food magazine: Innovations in collagen fermentation using MS technology

Innovations in collagen fermentation using MS technology

Explore the future of biofoods with experts Dan Merriman and Andrew Saarni in our latest webinar on collagen fermentation.

How about a 20 minute coffee break along with a bite of <a href="https://lcms.cz/products/627">Empower</a>? Here you go!
In this session we demonstrate how to create and use Customer Fields to generate workflows in Empower
A Custom Field is a user defined field (or column) of information in a table. Creating Custom Fields gives you the ability to create tailored sample identifiers and calculations beyond what Empower provides by default. During this series, we will explore the various types of Custom Fields that can be created and provide some practical examples.
Learn more and register!
<blockquote>
Presenter: Carsten Fischer (Informatics Product Marketing, EMEA, Waters Corporation)
</blockquote>

Waters - Informatics series #12 Adjust Empower very flexible to your specific needs

Adjust Empower very flexible to your specific needs

In this session we demonstrate how to create and use Customer Fields to generate workflows in Empower.

When it comes to chemical quantitative analysis, researchers often find themselves navigating multiple software packages. Mastering these tools to achieve the same level of expertise and reproducibility is a major pain point.
Further disruption to the workflow can occur when one needs to switch tools depending on the analytical technique or data format or even resort to using spreadsheets or in-house tools that require manual processes and are prone to error. 
To overcome these challenges, Wiley’s new KnowItAll Quantitation tool provides a single, easy-to-use tool to perform these tasks consistently in a vendor-neutral environment. 
<h4>In this session we will:</h4>
<ul>
<li>Introduce KnowItAll’s simple and intuitive quantitation process, its unique features, and how it can streamline and enhance your analytical workflow.
</li>
<li>Demonstrate through live examples the various methods supported by KnowItAll: external calibration, internal calibration, and standard addition analysis, and how to successfully quantify spectra of unknown concentration.
</li>
</ul>
<h4>This session is recommended for:</h4>
<ul>
<li>Scientists and analytical chemists who use AT-IR, IR, GC-MS, Raman, and UV-Vis techniques for spectral quantitative analyses.
</li>
<li>Professors and students engaged in analytical chemistry and quantitative analysis courses or lab work.
</li>
</ul>
<h4>About KnowItAll Solutions:</h4>
KnowItAll offers solutions to analyze and manage multiple types of spectral and chemical data in multiple file and instrument formats. KnowItAll&#39;s integrated toolsets eliminate the need for multiple software packages and increase overall lab efficiency. Combined with the comprehensive spectral reference database, KnowItAll provides chemists the most advanced technology available for spectral analysis.
<blockquote>
Presenter: Dr. Leanna Ergin (Assistant Editor, Wiley Science Solutions)
</blockquote>
<blockquote>
 Q&amp;A with:
Nadia Laschuk (Product Manager - Software and Databases - New Techniques)
Michelle D’Souza (Product Manager, Software Products)
</blockquote>

Wiley: Introducing KnowItAll Quantitation: Complete & Simplify Your Analytical Workflow

Introducing KnowItAll Quantitation: Complete & Simplify Your Analytical Workflow

We will Introduce KnowItAll’s simple and intuitive quantitation process and demonstrate through live examples the various methods supported by KnowItAll.

Introducing KnowItAll Quantitation

<h4>Translation of clinical metabolomics into routine applications</h4>
<blockquote>
Presenter: Prof. David Friedecký (Head of the Department of Clinical Biochemistry, University Hospital Olomouc, Czech Republic)
David Friedecký is an analytical chemist and professor of clinical biochemistry with over 20 years of experience in routine laboratory diagnostics. Currently, he is the Head of the Department of Clinical Biochemistry at the University Hospital Olomouc, Czech Republic. His expertise includes the clinical applications of liquid chromatography and mass spectrometry in the analysis of small molecules. He has developed or co-developed many FIA-MS and LC-MS methods that have found applications in national newborn screening, second-tier diagnostics and therapeutic drug monitoring. Scientifically, as the leader of the Clinical Mass Spectrometry Group at the Faculty of Medicine of Palacký University Olomouc, Czech Republic, he uses clinical metabolomics and lipidomics to understand the pathobiochemistry of diseases (mainly cardiovascular and neurodegenerative diseases, cancer or hyperuricaemia and gout), or in the development of advanced multicomponent methods that find application in clinical practice.
</blockquote>
<blockquote>
Presenter: Barbora Piskláková, MSc. (PhD candidate, Faculty of Medicine, Palacký University Olomouc, Czech Republic)
Barbora Piskláková is a Ph.D. candidate in Clinical Biochemistry and Medicinal Chemistry at the Faculty of Medicine, Palacký University Olomouc, Czech Republic and a researcher in the Clinical Mass Spectrometry Group. Her main research achievement was the development of a fast and easy-to-use multicomponent LC-MS method for the analysis of organic acids, acylglycines and acylcarnitines in urine as a panel capable of diagnosing more than 80 inherited metabolic disorders. Her work shows how challenging it is to bring metabolomics into clinical practice where the full analytical and clinical validation of this novel method took over 5 years and measurement of 800+ patient samples. Now, this method is considered the method of first choice in our diagnostic lab and has replaced the conventionally used GC-MS approach. Additionally, Barbora is using metabolomics in the study of cytidine metabolism in relation to cytarabine treatment for leukemia. She has also completed a 3-month internship at Oslo University Hospital, Norway at Katja Benedikte Prestø Elgstøen Metabolomics group.
</blockquote>

EMN Metabolomics Society: Translation of clinical metabolomics into routine applications

EMN December Webinar Series: "Translation of clinical metabolomics into routine applications"

Translation of clinical metabolomics into routine applications

From clinical metabolomics to routine applications

Get ready to dive into the thrilling world of process analytics - game show style!
Join us for an engaging and educational event as you showcase your knowledge by answering questions to discover how process analyzers can be equipped with titration, ion chromatography or spectroscopy to solve even the toughest challenges.
Our hosts, Elena Hagemann, Product Manager for Process Spectroscopy, and Kraig Kmiotek, Product Manager for Process Wet Chemistry, will test your knowledge while introducing the FUNdamentals of process analytics and unraveling practical insights that can be applied in real-world situations.
Whether you&#39;re a seasoned expert or just curious about the basics, this interactive event promises to educate and entertain, combining the fun of a game show with the science and engineering of process analytics.
Register now, don’t miss out on the end-of-the-year fun!
<blockquote>
Presenter: Kraig Kmiotek (Product Manager for Process Wet Chemistry, Metrohm USA)
</blockquote>
<blockquote>
Kraig Kmiotek is the Product Manager for Process Wet Chemistry at Metrohm USA and has a Bachelor of Science in Chemistry from Purdue University. Since joining the Process Analytics team, his focus has been on application work, analyzer design and programming and customer and internal training of process analyzers. He is currently responsible for understanding customer requirements, driving product and service development, and strategic product marketing and demand generation.
</blockquote>
<blockquote>
Presenter: Elena Hagemann (Product Manager for Process Spectroscopy, Metrohm USA)
</blockquote>
<blockquote>
Elena Hagemann is the Product Manager for Process Spectroscopy at Metrohm USA in Riverview, Florida. She holds a master’s degree in Analytical and Bioanalytical Chemistry from the Applied University of Aalen, Germany, where her master thesis focused on Karl Fischer Titration, NIR Spectroscopy, and Multivariate Data Analysis. Elena joined the Metrohm Group in August 2013 and started with Metrohm USA in January 2018, focusing on Raman and NIR Spectroscopy.
</blockquote>

Metrohm: Process Analytics Showdown: Putting the Fun in Fundamentals

Process Analytics Showdown: Putting the Fun in Fundamentals

The holiday season is approaching, and you are planning to switch off your lab? We have you covered!
Join our webinar and learn some easy protocols to prepare your analytical instruments to be switched off and some best practices around HPLC columns conditioning, so that you can start up again with maximum productivity.
<h5>Live from our demo lab in Milano, we will show you how to manage the following instruments:</h5>
<ul>
<li>1260/1290 Infinity II LC</li>
<li>LCMS Single Quad, Triple Quad and QTOF</li>
</ul>
Not all laboratories and not all instruments need a complete switch off, we will provide you a compass to find the right directions for your lab.
As a second part we will provide a comprehensive summary on how to protect your HPLC columns from early failure, clogging and performance loss and we will cover key tips and tricks to maximize your columns lifetime.
<blockquote>
Presenter: Scott Bollen (Business Development Manager, Agilent Technologies)
</blockquote>
<blockquote>
Scott specializes in small molecule LC columns, ranging from HPLC to UHPLC. Scott has worked at Agilent for 17 years. He started as a technical support manager, and now supports new LC products in R&amp;D as well as the entirety of the existing small molecule LC column portfolio in business development.
</blockquote>
<blockquote>
Presenter: Lorenzo Zingaro (LC/MS Product Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Lorenzo Zingaro studied at the University of Milan, obtaining a degree in Pharmaceutical Chemistry and Technology in 2004
</blockquote>
<blockquote>
He worked for six years in an analysis laboratory dealing with the development, validation and analysis of LC-MS methods, applied to chemical and biological markets. In 2013 he began his experience in Agilent Technologies Italia, working as an on-site service engineering for 5 years. Since 2018 he has held the role of Liquid Chromatography and Mass Spectrometry Product Specialist.
</blockquote>
<blockquote>
In recent years he has gained technical and applicative experience on the main LC and LCMS hardware and software platforms, participating in the introduction of new products and technologies on the market.
</blockquote>
<blockquote>
Presenter: Sophie Tirat (Application Engineer, Agilent Technologies)
</blockquote>
<blockquote>
Sophie Tirat obtained her PhD in Chemistry in 2015. She worked for 3 years as a LC-MS specialist in the field of drug discovery at the University of Lille, then for 4 years as analytical development specialist for a pharmaceutical company, dealing with the development and validation of LC-MS and GC-MS methods for food supplements and medical devices.
</blockquote>
<blockquote>
Sophie started her carrier in Agilent in October 2022 as an Application Engineer, focusing on performing demonstrations and developing new applications for the Applied Markets, using Agilent’s LC-MS and GC-MS instruments.
</blockquote>

Agilent Technologies: Preparing your Lab for the holiday season

Preparing your Lab for the holiday season

Learn some easy protocols to prepare your analytical instruments to be switched off and some best practices around HPLC columns conditioning.