<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

<h3>We're excited to announce that <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity Chromatography Software</a> version 10.1.2 is now available!</h3>This minor update expands Clarity’s portfolio with new control modules.<h4>Newly added Instrument control modules</h4><ul><li>Ecom auxiliary modules</li><li>HTA ICF control modules</li><li>Spark pump</li></ul><h4>Updated Instrument control modules</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/168">Ecom</a> HPLC</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/328">Knauer</a> HPLC</li><li>Spark ICF control modules</li><li>YoungIn Chromass HPLC</li></ul><blockquote>For a complete list of module changes, see the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">Changelog</a>. For more info about exciting new features directly in Clarity, explore what was introduced in the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5622">Clarity 10.1 release highlights</a>.Clarity can control 1000+ instruments; the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/controls">list can be previewed here</a>.</blockquote><h4>Supported Instruments in Clarity (DataApex) — Quick Guide</h4>Clarity Chromatography Software supports a wide range of chromatographs and peripherals via digital control (DD) and analog acquisition (A/D) using the Colibrick module.<h5>Gas Chromatographs (GC)</h5>Digital integration over LAN/RS232 (many via ICF):<ul><li>Agilent: 5890, 6890, 7890, 8860, 8890, Intuvo 9000</li><li>Shimadzu: GC-2010/2014, Nexis GC-2030</li><li>Thermo Fisher: TRACE 1300/1310</li><li>PerkinElmer: Clarus 400–690</li><li>Also supported: Dani, Ellutia, Young In Chromass, Apix, Fuli, GOW-MAC (selected models)</li></ul><blockquote>Note: Most Agilent 6890–8890 and Intuvo use LAN – ICF.</blockquote><h5>HPLC Systems</h5>Full system/module control:<ul><li>Agilent 1100–1290 Infinity II (ICF)</li><li>Shimadzu LC-10/20 and Nexera LC-40</li><li>PerkinElmer Flexar / NexSAR</li><li>Knauer Azura / BlueShadow / Smartline</li><li>Hitachi Chromaster / Primaide</li><li>Young In Chromass ChroZen series</li><li>Sykam S500–S600, Watrex Streamline, Hanon LC7000–7300</li></ul><h5>HPLC Modules (Pumps, Detectors, Ovens, Fraction Collectors)</h5><ul><li>Pumps: Agilent 1100–1290 families, Knauer, Shimadzu, PerkinElmer, Sykam, SSI, Ecom</li><li>Detectors: VWD/DAD(PDA)/RID/FLD/ELSD across Agilent, Shimadzu, Knauer, PerkinElmer, Young In Chromass, Ecom, etc.</li><li>Column Ovens: Agilent G1316x / G7116x, Shimadzu CTO-20/30/40, Knauer CT2.1, PerkinElmer Flexar</li><li>Fraction Collectors: Knauer FC 6.1, Teledyne FOXY series, Ecom ECF 2096, Waters Fraction Collector II</li></ul><h5>Autosamplers</h5><ul><li>Agilent GC ALS (7673/7683/7693/8697), HPLC ALS modules (G1313A–G7167C)</li><li>Shimadzu AOC-20 / AOC-5000+, SIL-10 / SIL-20 / SIL-40 series</li><li>CTC Analytics PAL, HTA autosamplers, Spark Alias/UHPLC, Knauer AS 6.1L</li><li>PerkinElmer Flexar / NexSAR AS</li></ul><h5>Other Devices</h5><ul><li>GC×GC modulators (e.g., Charles University GC×GC Modulator)</li><li>Valves &amp; switch modules (Agilent, Knauer, Shimadzu, VICI-Valco)</li><li>Degassers, flow monitors, managers, interfaces</li><li>LC-MS / GC-MS: Advion Expression CMS, Microsaic 4500 MiD, Markes BenchTOF (and others)</li><li>Balances (EA extension): Mettler Toledo, Sartorius</li></ul><h5>Analog Acquisition (Colibrick)</h5>A/D support for legacy or analog-output devices:<ul><li>GC: Agilent 5880/4890A, Varian 3xxx/3800/3900, PerkinElmer Clarus series, Thermo TRACE, Shimadzu GC-14/9A, Scion 43x/45x/83xx/85xx, etc.</li><li>HPLC Detectors: Waters 2487/2489, ESA Corona, Hitachi L-series, JASCO FP/UV, Ecom LCD series, many others</li><li>Elemental &amp; Amino Acid Analyzers: Carlo Erba/Costech ECS, Biochrom 20/30+</li></ul>If you are interested in a DEMO VERSION, contact us!

DataApex Clarity Chromatography software 10.1.2 just released!

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Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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SCIEX

Evosep Biosystems

Waters Corporation

Join this webinar to hear from Dr. Karl Oetjen and Dr. Craig Butt, who will share their recent research on nontargeted analysis of food products.
Discover how advanced analytical methods can detect unlisted, unexpected, and unwanted ingredients, leading to increased confidence throughout the supply chain, ultimately benefiting consumers.
This webinar looks at several sample types in the application of diagnostic ion screening to prioritise nontarget features for food safety analysis. Data from this study includes the analysis of supplements products from North American Gas Stations to determine what are the actual ingredients that “boost metabolism”, “elevates mood” and supply “good luck energy”. This study also looks at saffron and its origins along with an update on nontargeted screening of pesticides in food samples.
Discover the ingredients behind marketing slogans.
<ul>
<li>Understand why ZenoTOF technology is at the forefront of detecting the unknown, unexpected and unwanted at trace levels.
</li>
<li>Discover how mass spectrometry can be used to determine authenticity of expensive ingredients.
</li>
<li>Learn how nontargeted workflows can test the regulated and the non-regulated in pesticide assays.
</li>
<li>A chance to ask our expert speakers questions and these exciting workflows.
</li>
</ul>
<blockquote>
Presenter: Karl Oetjen (Senior Scientist, Technical Marketing, SCIEX)
</blockquote>
<blockquote>
Karl Oetjen is a Senior Scientist at SCIEX in the technical marketing group and has over 10 years of mass spectrometry experience. Before joining SCIEX, he completed his PhD at Colorado School of Mines (Golden, Colorado) in Hydrologic Science and Engineering under Dr. Chris Higgins. Karl’s dissertation research focused on nontarget characterisation of complex surfactant mixtures, including aqueous film forming foams. This work led to the discovery several novel per- and polyfluorinated alkyl substances (PFAS) that since have been found in variety of environmental samples and industrial chemicals. Since joining SCIEX, Karl has worked with numerous labs creating and implementing both regulated and unregulated quantitative and qualitative screening methods. 
</blockquote>
<blockquote>
Presenter: Craig Butt (Manager, Applied Markets, SCIEX)
</blockquote>
<blockquote>
Craig M. Butt is Manager of the Applied Markets group in the Global Technical Marketing division of SCIEX with over 20 years of mass spectrometry experience. Previously at SCIEX he was an applications scientist where he developed methods for targeted and non-targeted methods in the cannabis, environmental, food and beverage group. He obtained his Ph.D. in environmental chemistry at the University of Toronto, his thesis research investigated the fate of poly- and perfluorinated organic compounds (PFAS) in biological systems. He was a NSERC post-doctoral research fellow in the Nicholas School of the Environment at Duke University, later becoming a research scientist in the department.
</blockquote>

New Food:Enhancing food safety analysis with diagnostic Ion screening

Enhancing food safety analysis with diagnostic Ion screening

Several sample types in the application of diagnostic ion screening to prioritise nontarget features for food safety analysis.

Exposomics research studies the impact of environmental factors, including exposure to contaminants, on health.
Untargeted workflows for metabolomics and exposomics typically report hundreds of features belonging to diverse compound classes, identified with varying levels of confidence.
In this webinar, our expert speaker will discuss how to optimize your untargeted approaches on Zeno SWATH DIA using a modular workflow. With this approach, each subset of the full mass range can be individually adjusted to meet project-specific and user-defined goals, enhancing the performance and adaptability of untargeted MS tools for exposomics research.
Attend this webinar to: 
<ul>
<li>Discover exposomics, the insights it provides into human health and the role of mass spectrometry in this field
</li>
<li>Learn a rational approach to optimize chromatography and mass spectrometry parameters
</li>
<li>Make your method development efforts modular so they can be reused to meet different project-specific needs
</li>
<li>Find out how using Zeno SWATH DIA can benefit your metabolomics or exposomics research.
</li>
</ul>
<blockquote>
Presenter: Dr. Cristina Balcells Nadal (Research Associate, Imperial College London)
</blockquote>

Technology Networks: Optimizing SWATH-DIA for Exposomics and Metabolomics

Optimizing SWATH-DIA for Exposomics and Metabolomics

How to optimize your untargeted approaches on Zeno SWATH DIA using a modular workflow.

The 1st key-note presentation will explore the pursuit of enhanced chromatographic performance in low-flow applications.
Microfluidic pillar array columns offer a compelling alternative to traditional packed beds and monolithic columns, resulting in microfluidic separation beds with nanometer-scale control over geometry, enhancing quantitation accuracy and precision. The presentation focuses on a 5.5 cm separation channel housing radially elongated rectangular pillar shapes, ideal for high-throughput chromatographic separations. Integrated with advanced LC-MS instruments, the Vanquish Neo UHPLC system, and the latest Orbitrap mass spectrometers, this configuration achieves unparalleled quantitation accuracy and precision, handling up to 300 samples daily. 
The second part will take us to detection technologies. HPLC has seen the advent and often also decline of various techniques which were claimed to enable universal detection. The emphasis will be on the use of an instrumental technique that was published specifically for charged aerosol detection (CAD) and is referred to as inverse gradient with the goal to widely eliminate the influence of the mobile phase composition on the analyte response. Practical examples will address extractables &amp; leachables, but also very common excipients in biopharmaceutical formulations. The method uses multiple orthogonal detectors (UV, CAD, MS) combined with the inverse gradient technique to facilitate analysis of a chemically diverse range of analytes. 
Learning points:
<ul>
<li>How to achieve unparalleled quantitation accuracy and precision in high-throughput chromatographic separations 
</li>
<li>Detection technologies
</li>
</ul>
Who should attend:
<ul>
<li>Lab managers and directors 
</li>
<li>Principal scientists 
</li>
<li>Laboratory analysts
</li>
</ul>

Thermo Scientific: Leveraging innovative stationary phase architectures and multi-detection strategies in biopharma and proteomics research to enhance precision, efficiency, and results

Leveraging innovative stationary phase architectures and multi-detection strategies in biopharma and proteomics research to enhance precision, efficiency, and results

We will explore the pursuit of enhanced chromatographic performance in low-flow applications. The second part will take us to detection technologies.

Stationary phase & multi-detection in biopharma & proteomics

This webinar is your guide to get started with a fast and efficient, automated sample preparation protocol on the Opentrons OT-2.
Utilize protein aggregation capture (PAC) on magnetic microparticles in an end-to-end standardized and hands-off workflow, starting from protein lysate or plasma to peptide loaded on Evotips.
<h4>Why choose an automated workflow?</h4>
Automated sample preparation workflows have become a popular tool in proteomics laboratories for a number of reasons. With increased sample throughput on the Evosep One, you can prioritize numerous technical and biological replicates, adding robustness to downstream bioinformatic analyses.
By adopting full automation, you can gain precise control over protocols, ensuring reproducibility across samples and experiments. This minimizes batch effects, resulting in more reliable data for analysis and interpretation.
Furthermore, enhanced automation significantly reduces manual labor, enhancing reproducibility and providing researchers with more time for critical decision-making. This workflow also proves cost-efficient for large-scale applications, optimizing protein input for digestion.
<h4>AUTOMATED WORKFLOW FOR INTEGRATED SAMPLE DIGESTION AND EVOTIP LOADING FOR HIGH-THROUGHPUT CLINICAL PROTEOMICS</h4>
<blockquote>
Presenter: Anders H.Kverneland (MD &amp; Postdoctoral researcher at Center for Cancer Immune Therapy, Herlev hospital)
</blockquote>
Protein identification and quantification is an important tool for biomarker discovery. With the increased sensitivity and shortened the analysis tim​e of modern mass spectrometers, sample-preparation remains a bottleneck and source of variability when studying large cohorts. To address this issue, we evaluated the use of a simple integrated workflow on the Opentrons OT-2 robot enabling both sample digestion and Evotip loading. Our results demonstrate high sensitivity combined with both reproducibility and stability even with very low sample inputs. The integration of the digestion and Evotip loading also strongly reduced the amount of reagents needed for the sample preparation. Using the integrated workflow, we evaluate the effects of cancer immune therapy on the plasma proteome in metastatic melanoma patients.
<h4>STANDARDIZED AND FULLY AUTOMATED DEEP PROTEOME PROFILING OF PLASMA INTEGRATED WITH EVOSEP ONE ENABLES LARGE-SCALE CLINICAL COHORT ANALYSIS</h4>
<blockquote>
Presenter: Dorte Becker-Jensen (Application Manager at Evosep)
</blockquote>
This streamlined end-to-end workflow enables deep plasma profiling through the automated enrichment of membrane-bound vesicles and depletion of abundant proteins from a mere 4 μl of plasma. Integrated with the SCIEX ZenoTOF, it consistently identifies more than 3,500 protein groups, all with the ease of processing 30 samples per day at the push of a button.

Evosep: Elevate efficiency: The power of automation

Elevate efficiency: The power of automation

Get started with a fast and efficient, automated sample preparation protocol on the Opentrons OT-2.

PFAS, or per- and polyfluoroalkyl substances, have primarily been studied in the context of environmental contamination, but recent developments have led to exploring their impact in diverse areas such as food, bioclinical settings, personal care products, and pharmaceuticals.
This expert panel discussion will explore the lessons learned from environmental applications and how these insights can be transitioned to these other arenas. The conversation will navigate through the current challenges faced, examine technologies at our disposal, pinpoint unmet analytical needs, and map out next steps. In addition, an overview of the ever-evolving regulatory landscape will provide an important backdrop for the discussion.
By attending this presentation, you will:
<ul>
<li>Gain an understanding of the challenges involved when transitioning from environmental matrices.
</li>
<li>Recognize the current analytical solutions available and identify gaps that present opportunities for innovation.
</li>
<li>Understand the complexities and challenges in the regulatory landscape concerning PFAS analysis across diverse sectors.
</li>
<li>Be better equipped to develop an action plan for next steps in the journey of expanding PFAS analysis beyond its traditional environmental context.
</li>
</ul>
Who should attend
<ul>
<li>Anyone involved in PFAS analysis, method development, or research should consider joining this webinar. Questions outside the scope of the description are welcome and will be addressed if time allows.</li>
</ul>
<blockquote>
Presenter: Rock J. Vitale (Technical Director of Chemistry, Senior Principal at Environmental Standards, Inc.)
</blockquote>
<blockquote>
Mr. Vitale has over 40 years of analytical and QA experience. Specifically, he has six years of analytical experience performing analyses for organic and inorganic contaminants in a variety of media by instrumental and classical methods, including research and development of analytical methodologies. As a Principal of Environmental Standards, Mr. Vitale oversees a staff of approximately 40 QA Chemists - one of the nation’s largest. He is a recognized expert in the following fields: organic and inorganic analysis and data quality assessment (including high-resolution PCBs, dioxins/furans, and PFAS); laboratory auditing; preparation or third-party review of QAPPS for complex analytical programs; methods development to accommodate project-specific data quality objectives (DQOs); design of QA programs; and agency negotiations.
</blockquote>
<blockquote>
Presenter: Olga Shimelis (Analytical Chemistry R&amp;D Manager at the life science business of Merck KGaA, Darmstadt, Germany)
</blockquote>
<blockquote>
Dr. Olga Shimelis worked for MilliporeSigma in multiple R&amp;D roles at the Bellefonte, PA, USA site in the area of development of consumables for chromatographic separations. Olga holds a Ph.D. in Analytical Chemistry from the University of Oklahoma, USA.
</blockquote>
<blockquote>
Presenter: Emiliano De Dominicis (Chemistry Research Director at Mérieux NutriSciences Italia)
</blockquote>
<blockquote>
Emiliano is a researcher with over twenty years of experience in food and pharmaceutical safety, following both private and academic sectors, and he is now Director of Chemical Research - Project Manager at Mérieux NutriSciences. With a team dedicated to Research and Development in Analytical Chemistry, Emiliano is responsible for Mérieux NutriSciences&#39; R&amp;D lab, taking part in global research projects. Moreover, he actively participates in funded scientific projects (regional, national, and European) and scientific congresses as a speaker, and writes articles published in specialized scientific journals.
</blockquote>
<blockquote>
Presenter: Ruth Marfil-Vega (Senior Market Manager, Environmental at Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Dr. Ruth Marfil-Vega has 20 years of expertise in environmental chemistry and engineering in the US and Europe. She has worked with the EPA and the utilities industry to design and execute comprehensive solutions for addressing new environmental and water quality issues, including PFAS, regulated and unregulated DBPs, and other emerging contaminants. Ruth has continued working with environmental stakeholders since she joined Shimadzu five years ago to make sure that analytical instrumentation and workflows enable their success. She graduated from the University of Valladolid (Spain) with a BSc in Chemistry, and from the University of Cincinnati with a PhD in Environmental Science and Engineering.
</blockquote>
<blockquote>
Presenter: Erin Baker (Associate Professor at the University of North Carolina at Chapel Hill)
</blockquote>
<blockquote>
Erin S. Baker is an Associate Professor at the University of North Carolina at Chapel Hill. To date, she has published over 160 peer-reviewed papers utilizing different analytical chemistry techniques to study both environmental and biological systems. Over the last four years, Erin also helped grow the Females in Mass Spectrometry group, where she served as the Events Committee Chair from 2019-2022. She is currently serving as the Vice President of Education for the International Lipidomics Society, a mentor for Females in Mass Spectrometry, and an Associate Editor for the Journal of the American Society for Mass Spectrometry.
</blockquote>

Separation Science: Expanding PFAS Analysis Beyond Environmental Matrices

Expanding PFAS Analysis Beyond Environmental Matrices

Explore the lessons learned from environmental applications and how these insights can be transitioned to these other arenas.

Are you looking to transform your organization’s digital landscape? Does digital transformation seem like something that you aren’t able to attain just yet?
Join us for an engaging webinar where we delve into the Thermo Scientific Ardia Platform, a game-changing solution for scientists seeking to unlock the full potential of their data. This session will provide a comprehensive overview of the Ardia Platform, showcasing its advanced data analysis and connectivity capabilities. Explore how the Ardia Platform can accelerate your scientific discoveries, streamline your workflows, and enable you to make data-driven decisions with confidence. Don&#39;t miss this opportunity to revolutionize your research with the Ardia Platform.
Who should attend?
<ul>
<li>Researchers looking to better analyze their chromatography and mass spectrometry data </li>
<li>Anyone involved with driving digital transformation in their organization </li>
<li>Lab managers, IT professionals, laboratory system administrators, and business leaders</li>
</ul>
 What you will learn?
<ul>
<li>How the Ardia Platform offers connectivity, scalability, and security
</li>
<li>Core applications of the Ardia Platform
</li>
</ul>
<blockquote>
Presenter: Larry West (Senior Enterprise Software Solution Sales Specialist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Larry has worked with chromatography data systems for over 40 years. Today he is part of the Thermo Enterprise CDS Team and supports Chromeleon. He holds a bachelor’s degree in chemistry and began his work in chromatography at Whatman Inc in 1981. He enjoys the whole process of streamlining the needs of chromatographers to make their user experience the best it can be.
</blockquote>

Thermo Scientific: Simplifying digital transformation with the Thermo Scientific Ardia Platform

Simplifying digital transformation with the Thermo Scientific Ardia Platform

Thermo ScientificTM ArdiaTM Platform, a game-changing solution for scientists seeking to unlock the full potential of their data.

Digital transformation - Thermo Scientific Ardia Platform

Discover the ground-breaking technology that promises a whole new level of chromatographic performance in low-flow applications, making you rethink what&#39;s possible.
First, we&#39;ll show you how the latest advancements in column technology seamlessly integrate with the Vanquish Neo UHPLC system and cutting-edge Orbitrap mass spectrometers. Imagine achieving unparalleled accuracy and precision while handling up to 300 samples daily – this is the future of high-throughput chromatographic separations.
The 2nd Keynote part explores the ever-evolving landscape of HPLC detection techniques – specifically around universal detection! We’ll discuss what universal detection really means, delve into the power of Charged Aerosol Detection (CAD) and what advantages inverse gradient can bring to your analysis. This will all be demonstrated with practical examples addressing extractables &amp; leachables along with common biopharmaceutical excipients.
Learning points:
<ul>
<li>How to achieve unparalleled quantitation accuracy and precision in high-throughput chromatographic separations 
</li>
<li>Detection technologies
</li>
</ul>
Who should attend:
<ul>
<li>Lab managers and directors 
</li>
<li>Principal scientists 
</li>
<li>Laboratory analysts
</li>
</ul>
<blockquote>
Presenter: Katherine Lovejoy (Product Applications Specialist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Katherine Lovejoy, Ph.D. has been an application chemist focusing on charged aerosol detection at the Thermo Fisher Scientific HPLC factory site since 2016 and has a Ph.D. in inorganic chemistry from the Massachusetts Institute of Technology. She has experience as a chemist reviewer at the FDA and as a scientist at Los Alamos National Laboratory.
</blockquote>
<blockquote>
Presenter: Mauro De Pra (Product Application Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Mauro De Pra is Product Application Manager at Thermo Fisher Scientific. Since he joined the company in 2010, he focused on product development, application development and support, and product marketing. Before joining Thermo Fisher Scientific, he worked as Analytical Development Scientist at MSD. Mauro holds a PhD in analytical chemistry from the University of Amsterdam.
</blockquote>
<blockquote>
Presenter: Dr. habil. Frank Steiner (Senior Manager Product Applications &amp;Scientific Advisor, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Dr. Frank Steiner is currently managing an applications laboratory and coordinating scientific collaborations in the HPLC organization of Thermo Fisher Scientific. He received his PhD degree in Chemistry in 1995 from the Saarland University in Saarbrücken, Germany working on development of stationary phases for IC. He then became a postdoctoral research fellow at the CEA, Saclay in France focusing on elementary and isotopic analysis by IC and IC-ICP/MS in 1996. Frank returned to Saarland University in 1997 to conduct research on electro-driven separation, LC purification and MS coupling technologies and became an assistant professor in 2003, after receiving the university teaching credentials (habilitation).
</blockquote>
<blockquote>
In 2005 Frank joined Dionex GmbH in Germering, Germany now a part of Thermo Fisher Scientific, as the manager for LC systems. Frank played a significant role in developing and launching the Vanquish UHPLC systems. He published over 30 publications in refereed journals and more than 10 text book chapters on HPLC method development and optimization.
</blockquote>
<blockquote>
Presenter: Dr. Jeff Op de Beeck (Staff Scientist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Dr. Jeff Op de Beeck is currently Staff Scientist at Thermo Fisher Scientific’s R&amp;D facility in Ghent where micro pillar array technology is developed and produced. His work under the supervision of Prof. Gert De Smet and Prof. Wim De Malsche formed the basis for the current micro Pillar Array Column, which is branded by Thermo Fisher Scientific as the µPAC™. He holds a master’s degree in Biomedical Sciences from the University of Antwerp and a Ph.D. in Chemical Engineering from the University of Brussels.
</blockquote>
<blockquote>
Presenter: Natalie Van Landuyt (Thermo Fisher Scientific)
</blockquote>
<blockquote>
Natalie Van Landuyt is currently Scientist at Thermo Fisher Scientific’s R&amp;D facility in Ghent where micropillar array technology is developed and produced. With expertise in biotechnology, she made contributions as an R&amp;D Developer at PharmaFluidics and Senior Developer Immuno Assays at MyCartis. Earlier, she demonstrated leadership as a lab manager and became experienced in nanoLC as senior research associate during her tenure at Pronota, a Protein Biomarker discovery company. Natalie has a degree in Industrial Engineering Biochemistry and Medical Laboratory Technology from KHBO – Oostende/Brugge, Belgium.
</blockquote>

Thermo Scientific: HPLC Reloaded Day 1 – Quantitative Excellence in Pharma & Biopharma

Quantitative Excellence in Pharma & Biopharma

Discover the ground-breaking technology that promises a whole new level of chromatographic performance in low-flow applications.

We will examine how small changes to your vials &amp; closures can lead to a big impact to your recovery rates and reproducibility in your workflow.
Let´s be realistic: you will never get 100% of your analyte out of your sample handling container but we can help to secure your valid sample and improve the accuracy of your results.
During this webinar, we will examine developments and tips to:
<ul>
<li>eliminate or at least reduce adsorption in an autosampler vial</li>
<li>reduce the risk of moving your analyte out of the target pks/pka value for reliable LC analysis</li>
<li>eliminate sample loss due to evaporation by poor seals</li>
</ul>
We hope you will leave this webinar with an understanding on how to identify small fixes to reduce or eliminate sample loss for significant improvements. The session will finish with a short Q&amp;A and include a live chat with an expert.
<blockquote>
Presenter: Dr. Detlev Lennartz
</blockquote>
<blockquote>
Dr. Detlev Lennartz brings almost 30 years of experience in chromatography consumables (HPLC columns, GC columns, SPE cartridges, TLC products, and vials and closures). Twenty-four of those years he has been within product management developing and improving consumables for today’s researcher. His customer sales and technical support experience allows him to understand what challenges researchers face. The last twelve years Detlev has been the Thermo Scientific sample handling (autosampler vials, closures and well plates) worldwide product manager. Together, with a team of specialists, focusing on developing and launching new and innovative sample handling products, he hopes to share this knowledge to drive better efficiencies and improve results for researchers.
</blockquote>

VWR: How to reduce your sample loss to significantly improve your results

How to reduce your sample loss to significantly improve your results

We will examine how small changes to your vials & closures can lead to a big impact to your recovery rates and reproducibility in your workflow.

How to reduce your sample loss to improve your results

In this webinar, we will walk you through the use of Zeno SWATH DIA on the ZenoTOF 7600 system for high-performance proteomics analysis, highlighting the following:
<ul>
<li>Maximizing nanoflow chromatographic separation performance using IonOpticks Aurora SX series columns
</li>
<li>Achieving high sensitivity for protein detection and quantitation on low column loads of a complex digest standard, approximating single-cell protein levels
</li>
<li>Demonstrating depth of proteome coverage and quantitative fidelity with mixtures of digests from 3 different organisms, with protein ratios between mixtures accurately quantified using a label-free quantitation (LFQ) strategy
</li>
</ul>
<blockquote>
Presenter: Patrick Pribil (SCIEX)
</blockquote>

SCIEX: Pushing the boundaries of depth-of-coverage for nanoflow proteomics using Zeno SWATH DIA and IonOpticks Aurora SX series columns

Pushing the boundaries of depth-of-coverage for nanoflow proteomics using Zeno SWATH DIA and IonOpticks Aurora SX series columns

Using Zeno SWATH DIA and IonOpticks Aurora SX columns

Discover a new tool for multi projects search in Empower. This session shows you how you can use a new tool for answering questions that took a lot of effort in the past.

Waters Corporation: Waters Empower search tool

Empower search tool

This session shows you how you can use a new tool for answering questions that took a lot of effort in the past. 