<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

<h3>We're excited to announce that <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity Chromatography Software</a> version 10.1.2 is now available!</h3>This minor update expands Clarity’s portfolio with new control modules.<h4>Newly added Instrument control modules</h4><ul><li>Ecom auxiliary modules</li><li>HTA ICF control modules</li><li>Spark pump</li></ul><h4>Updated Instrument control modules</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/168">Ecom</a> HPLC</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/328">Knauer</a> HPLC</li><li>Spark ICF control modules</li><li>YoungIn Chromass HPLC</li></ul><blockquote>For a complete list of module changes, see the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/version_history?product=1">Changelog</a>. For more info about exciting new features directly in Clarity, explore what was introduced in the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5622">Clarity 10.1 release highlights</a>.Clarity can control 1000+ instruments; the <a target="_blank" rel="noopener noreferrer" href="https://www.dataapex.com/controls">list can be previewed here</a>.</blockquote><h4>Supported Instruments in Clarity (DataApex) — Quick Guide</h4>Clarity Chromatography Software supports a wide range of chromatographs and peripherals via digital control (DD) and analog acquisition (A/D) using the Colibrick module.<h5>Gas Chromatographs (GC)</h5>Digital integration over LAN/RS232 (many via ICF):<ul><li>Agilent: 5890, 6890, 7890, 8860, 8890, Intuvo 9000</li><li>Shimadzu: GC-2010/2014, Nexis GC-2030</li><li>Thermo Fisher: TRACE 1300/1310</li><li>PerkinElmer: Clarus 400–690</li><li>Also supported: Dani, Ellutia, Young In Chromass, Apix, Fuli, GOW-MAC (selected models)</li></ul><blockquote>Note: Most Agilent 6890–8890 and Intuvo use LAN – ICF.</blockquote><h5>HPLC Systems</h5>Full system/module control:<ul><li>Agilent 1100–1290 Infinity II (ICF)</li><li>Shimadzu LC-10/20 and Nexera LC-40</li><li>PerkinElmer Flexar / NexSAR</li><li>Knauer Azura / BlueShadow / Smartline</li><li>Hitachi Chromaster / Primaide</li><li>Young In Chromass ChroZen series</li><li>Sykam S500–S600, Watrex Streamline, Hanon LC7000–7300</li></ul><h5>HPLC Modules (Pumps, Detectors, Ovens, Fraction Collectors)</h5><ul><li>Pumps: Agilent 1100–1290 families, Knauer, Shimadzu, PerkinElmer, Sykam, SSI, Ecom</li><li>Detectors: VWD/DAD(PDA)/RID/FLD/ELSD across Agilent, Shimadzu, Knauer, PerkinElmer, Young In Chromass, Ecom, etc.</li><li>Column Ovens: Agilent G1316x / G7116x, Shimadzu CTO-20/30/40, Knauer CT2.1, PerkinElmer Flexar</li><li>Fraction Collectors: Knauer FC 6.1, Teledyne FOXY series, Ecom ECF 2096, Waters Fraction Collector II</li></ul><h5>Autosamplers</h5><ul><li>Agilent GC ALS (7673/7683/7693/8697), HPLC ALS modules (G1313A–G7167C)</li><li>Shimadzu AOC-20 / AOC-5000+, SIL-10 / SIL-20 / SIL-40 series</li><li>CTC Analytics PAL, HTA autosamplers, Spark Alias/UHPLC, Knauer AS 6.1L</li><li>PerkinElmer Flexar / NexSAR AS</li></ul><h5>Other Devices</h5><ul><li>GC×GC modulators (e.g., Charles University GC×GC Modulator)</li><li>Valves &amp; switch modules (Agilent, Knauer, Shimadzu, VICI-Valco)</li><li>Degassers, flow monitors, managers, interfaces</li><li>LC-MS / GC-MS: Advion Expression CMS, Microsaic 4500 MiD, Markes BenchTOF (and others)</li><li>Balances (EA extension): Mettler Toledo, Sartorius</li></ul><h5>Analog Acquisition (Colibrick)</h5>A/D support for legacy or analog-output devices:<ul><li>GC: Agilent 5880/4890A, Varian 3xxx/3800/3900, PerkinElmer Clarus series, Thermo TRACE, Shimadzu GC-14/9A, Scion 43x/45x/83xx/85xx, etc.</li><li>HPLC Detectors: Waters 2487/2489, ESA Corona, Hitachi L-series, JASCO FP/UV, Ecom LCD series, many others</li><li>Elemental &amp; Amino Acid Analyzers: Carlo Erba/Costech ECS, Biochrom 20/30+</li></ul>If you are interested in a DEMO VERSION, contact us!

DataApex Clarity Chromatography software 10.1.2 just released!

Article

Product

Application

Academy

Software

Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

DataApex

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Bruker

Phenomenex

SelectScience

C&EN

Waters Corporation

Key Learning Points:
<ul>
<li>Learn about types of tubing and fittings and making the best connections.
</li>
<li>Make sense of how to select the correct tube, properly cutting it and choosing the right fitting. 
</li>
<li>Understanding extra-column volume and what are Zero-Dead Volume connections.
</li>
<li>Tips and tricks to diagnose and troubleshoot poor tubing and/or fitting choices.
</li>
</ul>
Who Should Attend:
<ul>
<li>Any LC users wanting to deepen their knowledge of LC tubing and connections, including troubleshooting, to smoothly run their LC experiments</li>
</ul>
<blockquote>
Presenter: Lucia Geis Asteggiante (Senior Technical Specialist, Phenomenex)
</blockquote>
<blockquote>
Lucia is a Senior Technical Specialist that has recently joined Phenomenex’s Global Technical Team. She has a broad experience in the field of Analytical Chemistry with a particular focus on LC chromatography coupled with mass spectrometry. Lucia comes from a strong academic and regulatory background with experiences ranging from method development and analysis of small molecules to large protein complexes. She gained her Ph.D. at the University of Maryland - College Park, USA and did her post-doctoral work at the University of Oxford, UK. During her time as a researcher, she has authored/co-authored 23 scientific publications and 2 book chapters.
</blockquote>

Phenomenex: Spooktacular Solutions for LC Tubing and Connections

Spooktacular Solutions for LC Tubing and Connections

Learn about types of tubing and fittings and making the best connections.

Would you like to know what the new trends in LC are that could help you to achieve better and greener science at the same time?
In this webinar, Dr. Andreas Otto, Agilent Technologies, will discuss how innovation and sustainability go together, and what can be done to reduce the environmental impact of your lab with significant enhancements on scientific outcomes. Topics covered will include columns and supplies selection to reduce run times and its impact on solvent and energy consumption, the different LC techniques from supercritical fluid chromatography, online LC, and a new feed injection sampler that can improve standard LC analysis.
Information about free tools that can make the maximum impact for the minimal change will also be shared.
Key learning objectives
<ul>
<li>Learn how to achieve greener and better LC analysis at the same time
</li>
<li>Discover new LC trends that help you to achieve your goals
</li>
<li>Find out how feed injection mode can help you to perform better chromatography
</li>
<li>Explore the tools that are available so you can start to make a difference immediately
</li>
</ul>
Who should attend?
<ul>
<li>Lab Analysts who have sustainability goals for their lab
</li>
<li>LC users willing to improve their analysis
</li>
<li>Chromatographers who would like to access useful tools
</li>
</ul>
<blockquote>
Presenter: Dr. Andreas Otto (Product Marketing Manager, Liquid Phase Separation Division, Agilent Technologies)
</blockquote>
<blockquote>
Dr. Andreas Otto has worked at Agilent Technologies since May 2018 serving in different roles. In his current position, he leads a technical product marketing team where he coordinates activities for sustainability topics in communication and product development, including third party accreditation of the InfinityLab LC portfolio for the My Green Lab® ACT labels. He is a biochemist by training and has a PhD in Microbiology. Before joining Agilent, he worked with LC and LC/MS for more than 15 years.
</blockquote>
<blockquote>
Presenter: Carrie Haslam (Associate Editor, SelectScience)
</blockquote>
<blockquote>
Dr. Carrie Haslam is an Associate Editor at SelectScience, playing a key role in content production and specializing in Materials Science, Alzheimer’s disease and Clinical Diagnostics.
</blockquote>
<blockquote>
Carrie completed a Ph.D. from The University of Plymouth, where she developed graphene-based biosensors for the early diagnosis of Alzheimer’s disease.
</blockquote>

SelectScience: Do your objectives to go greener in LC seem difficult to achieve?

Do your objectives to go greener in LC seem difficult to achieve?

How innovation and sustainability go together, and what can be done to reduce the environmental impact of your lab.

Do your objectives to go greener in LC seem difficult?

As studies on PFAS continue to advance, the analytical technology used to support research and testing continues to advance.
Detection requirements for PFAS have been getting more challenging as advisory and regulatory limits are constantly being updated. Not only a highly sensitive LC-MS/MS platform essential, but background contamination must be controlled. We will discuss advances in LC-MS/MS technology with enhancements in negative ion sensitivity from the Xevo TQ Absolute Mass Spectrometer, as well as tools for data management with waters_connect software, and provide insights on controlling laboratory background. Waters comprehensive PFAS analysis support allows for detection of target compounds at ultra-trace concentrations along with easier, more robust, and accurate options as PFAS analysis continues into the future.
Key Learning Topics
<ul>
<li>Improvements in sensitivity using negative ion detection
</li>
<li>Developing and successfully implementing an LC-MS/MS method
</li>
<li>Tools for managing data (isomers, isotopes and more)
</li>
</ul>
Who should attend
<ul>
<li>PFAS Program Managers
</li>
<li>Laboratory Managers
</li>
<li>Analytical Scientists
</li>
<li>Quality Assurance Managers
</li>
</ul>
<blockquote>
Presenter: Gordon Fujimoto (PhD Group Leader, Chemical Analysis Applications, Waters Corporation)
</blockquote>
<blockquote>
Gordon Fujimoto is in Waters&#39; Americas mass spectrometry demonstration lab. He has worked on customer applications for twenty years for Waters. Currently, he is working with customers in the food, environment and materials areas on mass spectrometry applications.
</blockquote>

Waters Corporation: Trace-level Targeted Analysis for PFAS: The Challenges, and Best Practices for Success

Trace-level Targeted Analysis for PFAS: The Challenges, and Best Practices for Success

Advances in LC-MS/MS technology with enhancements in negative ion sensitivity from the Xevo TQ Absolute Mass Spectrometer.

Trace-level Targeted Analysis for PFAS - Best Practices

Mass Spectrometry Imaging (MSI) is a technique that allows chemical information to be obtained directly or via the use of matrices from a wide range of surfaces.
MS Imaging provides information on the spatial distribution of molecules at or near the surface. Imaging provides beautiful and information-rich images that allow scientists to answer the fundamental question: “Where is this component in my sample?”
In this webinar series, we will examine the basics of MS imaging, compare the techniques of untargeted and targeted MS Imaging and the information that can be gained from each, and end with an interview from Dr. Ryan Leib (Stanford University) and Dr. Roy Martin (Waters Corporation) on the practicalities of setting up a MS Imaging Core lab.
Who Should Attend: 
<ul>
<li>Any scientist, lab managers or lab directors looking to expand (or refresh) their knowledge of MS Imaging.</li>
</ul>
<h4>Part Three – October 31st, 2023: Building an MS Imaging Facility</h4>
In the final session of the series, we will have a roundtable with Dr. Ryan Leib (Stanford University) and Dr. Roy Martin (Waters Corporation) where we will discuss the steps necessary to set up an MS imaging core lab and answer questions such as: “who runs this type of lab?” and “who are the main customers?”.
<blockquote>
Presenter: Roy Martin (Principal Field Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Dr. LeRoy B. Martin, III is the Principal Field Marketing Manager for Biological Mass Spectrometry at Waters Corporation. His primary role is developing and promoting advanced mass spectrometry solutions in the Americas. He is also charged with evaluating and integrating new technologies and methods in collaborations and product development. Most recently, his focus has been on molecular imaging mass spectrometry and implementation of advances in ion mobility technology. Other research interests include complex mixture quantitative proteomics and structural biology using mass spectrometry. Roy received a BS in Chemistry from Davidson College and a Ph.D. in Analytical Chemistry from North Carolina State University.
</blockquote>
<blockquote>
Presenter: Ryan Leib (Director of Research, Stanford University Mass Spectrometry Laboratory)
</blockquote>
<blockquote>
Ryan Leib is Director of Research for the Stanford University Mass Spectrometry Laboratory. Ryan received his B.S. in Biochemistry from the University of Tulsa (Tulsa, OK), with a focus on LC/MS methods development for natural products discovery. In 2010, he received his Ph.D. in Chemistry from the University of California at Berkeley investigating ionic cluster formation and energetics in Professor Evan Williams’ mass spectrometry laboratory. Prior to joining SUMS in 2014, Ryan developed mass spectrometric analyses for complex mixtures as a Project Leader at SRI International, a non-profit contract R&amp;D firm based in Silicon Valley.
</blockquote>
<blockquote>
Presenter: Kieron Faherty, Ph.D. (Separations and Mass Spectrometry Field Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Kieron Faherty is the Separations and Mass Spectrometry Field Marketing Manager for the Americas at Waters Corporation. He received his B.Sc. (Hons) in Chemistry from the University of Reading (Reading, Berkshire, UK) and his doctorate in Chemistry from the University of Massachusetts (Amherst, MA). He has held several field-based positions at Waters, most recently as a Field Applications Specialist focused on biopharmaceutical applications using Time-of-Flight mass spectrometry instrumentation.
</blockquote>

Waters Corporation: Building an MS Imaging Facility

Building an MS Imaging Facility

The steps necessary to set up an MS imaging core lab and answer questions such as: “who runs this type of lab?” and “who are the main customers?”.

Per- and polyfluoroalkyl substances (PFAS) have been a hot topic for the past few years, and substances of interest for decades.
Recently, the European Food Safety Authority (EFSA) established tolerable weekly intakes for four PFAS substances, leading to maximum limits within the European Union for these compounds in fish, meat, and eggs. This development means that robust and sensitive analytical methods for PFASs are in high demand.
Wageningen Food Safety Research (WFSR) has developed several methods to serve the different purposes of PFAS analysis: official food control, monitoring at low levels for risk assessments, and research into additional substances and matrices. In this webinar, Dr. Kerstin Krätschmer and Dr. Richard Jack will give an overview of the methods, common challenges, the validation scheme described in the guidance document published by the European Union Reference Laboratory (EURL) for POPs in feed and food, and some analysis results in food and produce from the Netherlands.
Key learning objectives
<ul>
<li>Learn about PFAS sample preparation and analysis challenges from food matrices
</li>
<li>Understand how to ensure performance requirements for European Reference Laboratory requirements
</li>
</ul>
Who should attend?
<ul>
<li>Scientists and lab technicians involved in PFAS analysis
</li>
<li>Food safety analysts
</li>
<li>Food manufacturers that wish to learn more about PFAS analysis
</li>
</ul>
<blockquote>
Presenter: Kerstin Krätschmer, PhD (Wageningen Food Safety Research)
</blockquote>
<blockquote>
Dr. Kerstin Krätschmer is a state examined food chemist who studied food chemistry (MSc) at Bergische Universität Wuppertal, Germany and completed legal training at the State Institute for Chemical and Veterinary Analysis Krefeld (CVUA-RRW) and the Consumer Protection Authority Düsseldorf. After finishing her PhD at the European Union Reference Laboratory for halogenated Persistent Organic Pollutants (POPs) in Freiburg and University of Hohenheim in Stuttgart, Germany, she became project leader of the National Reference Laboratory for halogenated POPs of the Netherlands at Wageningen Food Safety Research. Her main focus lies on POPs in food and feed, but also includes potential food safety risks within the circular economy.
</blockquote>
<blockquote>
Presenter: Dr. Richard Jack (Phenomenex, Inc.)
</blockquote>
<blockquote>
Dr. Richard Jack is the Global Market Development manager for the environmental and food markets at Phenomenex corporation. He has over 18 years’ experience with chromatography and mass spectrometry for the environmental, semiconductor, chemical, and pharmaceutical industries. He is a former EPA Scientific Advisor for the EPA’s panel on Hydraulic Fracturing, a coauthor for EPA 557 and 557.1 along with ASTM D8001 and updates to D4327 and D6919 methods. He is currently the Second Vice Chairman for the ASTM D19 subcommittee on water analysis. He received his PhD in biochemistry and anaerobic microbiology from Virginia Tech University, USA and he has a Master’s in ecology from the University of Tennessee.
</blockquote>
<blockquote>
Presenter: Ellen Simms (Assistant Editor, SelectScienc)
</blockquote>
<blockquote>
Ellen studied biological sciences at the University of the West of England, UK, before completing a master&#39;s in epidemiology from the University of Bristol. As a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

SelectScience: Strategies to address monitoring and compliance testing of PFAS in food

Strategies to address monitoring and compliance testing of PFAS in food

Overview of the methods, common challenges, the validation scheme described in the guidance document published by the European Union Reference Laboratory.

Strategies to address monitoring and compliance testing

Ultrapure water (UPW) is crucial for the production of superior-quality semiconductors, but ensuring final product quality can be a challenge without the right tools.
UPW undergoes extensive and costly purification, utilizing various physical and chemical treatments to meet increasingly rigorous purity standards. In the face of global water shortages, efficient UPW production is essential. Water recycling facilities at semiconductor plants are tasked with addressing this concern, regenerating wastewater back into UPW. However, the integrity of regenerated UPW must be verified to ensure final product quality. This is where online ion chromatography provides a novel advantage: detecting trace anions, cations, and silica contaminants in real-time down to parts-per-trillion levels. 
Join us for a conversation with two industry experts: Ben Greenwood, Senior Manager at Micron Technology&#39;s Chemistry Lab, and Dariana Martinez, Product Application Specialist at Metrohm USA. Together, they will delve into the pressing water challenges currently faced by the semiconductor industry. Additionally, they will shed light on the pivotal role of ultrapure water and the significance of real-time ion chromatography to ensure quality control. 
Key Learning Objectives:
<ul>
<li>Understand the pivotal role of ultrapure water (UPW) in semiconductor manufacturing and its direct impact on product quality and performance. 
</li>
<li>Gain insights into the scale of water consumption in the semiconductor industry and the pressing challenges faced in meeting increasingly rigorous purity standards. 
</li>
<li>Discuss the advanced analytical capabilities of online ion chromatography, and learn how it accurately measures anions, cations, and silica contaminants to verify UPW purity.
</li>
</ul>
Who Should Attend:
<ul>
<li>Anyone interested in leveraging automation for process optimization and near-real-time data analytics
</li>
<li>Process engineers and plant operators responsible for analytical testing and QA/QC Managers responsible for ensuring maximum product quality
</li>
<li>Those involved in adopting or implementing Industry 4.0 initiatives and/or focused on developing newer, more efficient manufacturing processes
</li>
</ul>
<blockquote>
Presenter: Ben Greenwood (Senior Manager, Micron Technology&#39;s Chemistry Lab)
</blockquote>
<blockquote>
Presenter: Dariana Martinez (Product Application Specialist, Metrohm USA)
</blockquote>
<blockquote>
Presenter: Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)
</blockquote>

C&EN: Mastering UPW Quality with Real-Time Trace Ion Detection

Mastering UPW Quality with Real-Time Trace Ion Detection

The pressing water challenges currently faced by the semiconductor industry.

In less than twenty minutes we are going to show you how you can speed up method development by 30 % AND increase your assay precision 10-fold.
If you have ever:
<ul>
<li>had to troubleshoot poor peak shape</li>
<li>suffered from irreproducible results</li>
<li>lacked the resolution you need</li>
</ul>
Then this webinar is for you.
Our latest family of columns have been designed for out-of-the-box performance, maximizing your chance of getting it right first time. We have done this by packing our most reproducible and stable particles into new, high-performance hardware, which mitigates many of the challenges you currently face, whilst still providing the selectivity you are used to. These columns are available for both UHPLC and HPLC systems. A major pharmaceutical company recently described these columns as a &quot;non-negotiable&quot; part of their workflow.
<blockquote>
Presenter: Hazel Dickson (Market Development Manager Europe, Waters Corporation)
</blockquote>

Waters Corporation: Speed up HPLC method development by 30 %

Speed up HPLC method development by 30 %

How you can speed up method development by 30 % AND increase your assay precision 10-fold.

Metabolomics refers to the comprehensive measurement of small molecules in biofluids by either mass spectrometry (MS) or nuclear magnetic resonance (NMR) with the aim of covering multiple KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, exposome products, and chemical reactions to provide new insights into disease etiologies.
Lipidomics is a subset of metabolomics focused specifically on the analysis of lipid species. MS-based metabolomics and lipidomics generally require the use of liquid chromatography to separate metabolites based on polarity and high-resolution MS to accurately measure the mass-to-charge (m/z). The combination of retention time and m/z accuracy provides a reliable method to identify metabolites, which is critical for making disease marker discoveries.
In this webinar, Dr. Timothy Garrett, associate professor at the University of Florida, will discuss the clinical translation of metabolomics using examples of different diseases including rare diseases, cancer, and infectious diseases. He will explain how critical chromatography is to reproducibility and metabolite identification, and how new monodisperse columns can aid in separation and identification.
Key learning objectives
<ul>
<li>Learn the basics of applied metabolomics using UHPLC-HRMS/MS
</li>
<li>Understand how metabolomics can be used for developing new diagnostic tests
</li>
<li>Understand the potential of metabolomics and the current limitations
</li>
<li>Explore the role of chromatography in metabolomics
</li>
<li>Learn how utilizing monodisperse fully porous particles can improve your current metabolomics method
</li>
</ul>
Who should attend?
<ul>
<li>LC scientists looking for more efficiency for their metabolomic separations
</li>
<li>Scientists looking to improve their current HPLC/UHPLC separations for metabolomics
</li>
<li>Chromatographers looking to start developing new metabolomics based HPLC/UHPLC based methods
</li>
</ul>
<blockquote>
Presenter: Timothy J. Garrett (Associate Professor, University of Florida)
</blockquote>
<blockquote>
Dr. Timothy Garrett is an Associate Professor and Chief of Experimental Pathology in the Department of Pathology, Immunology and Laboratory Medicine at the College of Medicine at The University of Florida. He received his undergraduate degree from the University of Georgia in Chemistry and his PHD in Chemistry from the University of Florida. He joined the University of Florida in 2006, where he has developed a research program in metabolomics, lipidomics, and small molecule quantitation for clinical and biological research using mass spectrometry approaches. He is also currently Co-Editor-in-Chief for the Journal of Mass Spectrometry and Advances in the Clinical lab.
</blockquote>
<blockquote>
Presenter: Carrie Haslam (SelectScience)
</blockquote>
<blockquote>
Dr. Carrie Haslam is an Associate Editor at SelectScience, playing a key role in content production and specializing in Materials Science, Alzheimer’s disease and Clinical Diagnostics. Carrie completed a Ph.D. from The University of Plymouth, where she developed graphene-based biosensors for the early diagnosis of Alzheimer’s disease.
</blockquote>

SelectScience: The role of chromatography and monodisperse particles in mass spectrometry-based metabolomics for disease detection

The role of chromatography and monodisperse particles in mass spectrometry-based metabolomics for disease detection

The clinical translation of metabolomics using examples of different diseases including rare diseases, cancer, and infectious diseases.

Chromatography and monodisperse particles in MS metabolomics

Over the last few years, spatial biology has refined the understanding of coordinated biological processes that govern how systems both thrive and become vulnerable to disease.
The latest tools offer advanced images that track the location and types of proteins and RNA across tissue microdomains and cellular phenotypes using a variety of detection techniques but lack the ability to see other post-transcriptional processes defined by metabolites and glycans. MALDI Imaging is a true multiomics technique that allows for spatial determination of metabolites and lipids in tissue, as well as expanding targeted analysis to small molecules, glycans, and proteins. The addition of MALDI-2 and microGRID technology enables imaging for maximum biological information at previously unreachable sensitivity and robust (sub)cellular resolution.
This webinar will introduce how Bruker’s new MALDI HiPLEX-IHC Imaging workflow delivers greater speed and field-of-view for multiplexed protein imaging in tissue, while providing thousands of untargeted molecular images that enable deeper tissue subtyping. The combination of protein spatial localization combined with metabolites and glycans offers a new pathway for coordinated biomarker discovery to complement existing spatial techniques.
What to expect?
<ul>
<li>Live presentation from Bruker’s experts to see ease of use of the timsTOF fleX platform, new MALDI HiPLEX-IHC workflows and highlighting user stories, including focus on improved spatial resolution with microGRID, improved sensitivity with MALDI-2, improved separation selectivity with trapped ion mobility and comprehensive software solutions</li>
</ul>
Who should attend?
<ul>
<li>Anyone using or interested in using tools for spatial biology discovery or translation
</li>
<li>Scientists &amp; researchers interested in expanding their workflows to the spatial dimension, scientists working with spatial distribution of small molecule biologics
</li>
<li>MALDI Imaging users wanting to expand their workflows
</li>
<li>Anyone interested in learning more about MALDI Imaging capabilities
</li>
</ul>
<blockquote>
Presenter: Kate Stumpo, Ph.D. (Senior Market Manager Imaging, Bruker, Billerica, MA, USA)
<ul>
<li>Introduction and welcome to MALDI Imaging </li>
<li>Spatial biology meets Mass Spectrometry in cancer research </li>
<li>MALDI Imaging utility in neurological research</li>
</ul>
</blockquote>
<blockquote>
Presenter: Corinna Henkel, Ph.D. (Application Scientist Imaging, Bruker, Bremen, Germany)
<ul>
<li>MALDI HiPLEX-IHC workflow technical introduction</li>
<li>Software solutions for multiomics integration</li>
</ul>
</blockquote>
<blockquote>
Presenter: Bjoern Wendik, Ph.D. (Business Development Manager, Bruker, Bremen, Germany)
<ul>
<li>Bridging non-MS imaging to the MALDI world</li>
<li>Automation, workflow and relevance for future clinical applications</li>
</ul>
</blockquote>

Bruker: Redefining Hiplexed Spatial Multiomics

Redefining Hiplexed Spatial Multiomics

How Bruker’s new MALDI HiPLEX-IHC Imaging workflow delivers greater speed and field-of-view for multiplexed protein imaging in tissue.

Oligonucleotides continue to gain in popularity for a wide range of applications, from gene-based therapeutics to targeted crop protection.
Robust analytical tools and solutions are required to speed up development to meet compliance and regulatory requirements.
Sometimes traditional chromatographic LC-UV generated data is simply not enough, and orthogonal detection techniques like LC-MS provide scientists with greater confidence in getting the right result first time. 
Join us for this demonstration of a typical oligonucleotide impurities workflow using the ACQUITY Premier system with the ACQUITY QDa Mass Detector. 
<h5>Key learning objectives:</h5>
<ul>
<li>LCMS System overview and key hardware features</li>
<li>Practical tips and maintenance of the ACQUITY QDa detector</li>
<li>Impurities workflow overview, from sample setup, processing and reporting data in compliant Empower CDS software</li>
<li>Benefits of mass data for peak tracking and identification</li>
</ul>
Who should attend? 
<ul>
<li>Scientists in pharmaceutical, chemical, clinical, contract research organizations (CRO), cosmetics and agrochemical industries, universities, and governmental laboratories</li>
<li>Chromatographers wanting to transition to LC-MS from traditional LC-UV workflows</li>
<li>Anyone with an interest in mass spectrometry who wants to learn more</li>
<li>If you have any questions or would like to learn more please get in touch.</li>
</ul>
<blockquote>
Presenter: Victoria van Kuijk (Principal Applications Chemist, Waters Corporation)
</blockquote>
<blockquote>
Victoria van Kuijk works for the Waters UK and Ireland Demo lab, based in the South of the UK. Victoria is an experienced scientist specializing in LC instrumentation and separations method development, providing application consultation and support (Pharma/BioPharma).
</blockquote>
<blockquote>
Through previous roles within Waters, Victoria has acquired extensive experience and knowledge of Waters Empower CDS. She holds a Bachelor of Science (Hons) degree in Applied Chemistry from Nottingham Trent University.
</blockquote>

Waters Corporation: Benefits of Adopting Routine Mass detection in Traditional LC-UV Workflows for the Analysis of Synthetic Oligonucleotides

Benefits of Adopting Routine Mass detection in Traditional LC-UV Workflows for the Analysis of Synthetic Oligonucleotides

Join us for this demonstration of a typical oligonucleotide impurities workflow using the ACQUITY Premier system with the ACQUITY QDa Mass Detector. 