Per- and polyfluoroalkyl substances (PFAS) have become a major global concern due to their persistence, bioaccumulation, and potential health impacts. With thousands of known PFAS compounds, and new variants continuing to emerge, identifying and quantifying them across complex environmental and biological samples is an ongoing analytical challenge.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg" alt="Thermo Fisher Scientific: From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg 245w," sizes="100vw" width="1024" height="574"><h3>Why PFAS analysis matters</h3>PFAS are used in everything from firefighting foams to non-stick coatings, yet their chemical stability means they persist in the environment and accumulate in living organisms. Regulators and scientists around the world are racing to better understand their distribution, fate, and toxicity.But with tens of thousands of PFAS, many without available reference standards, traditional targeted analysis alone isn’t enough. Analysts increasingly rely on non-targeted analysis (NTA) workflows powered by high-resolution accurate-mass (HRAM) mass spectrometry and advanced software to uncover the full picture.<h3>Addressing the challenges of PFAS identification</h3>Successful non-targeted PFAS analysis requires overcoming several analytical hurdles:<ol><li>Acquiring high-quality MS¹ and MS² data with ultra-high mass resolution and accuracy.</li><li>Detecting low-abundance PFAS hidden within thousands of background compounds.</li><li>Leveraging comprehensive spectral libraries and databases for confident identification.</li><li>Applying data filtering and scoring tools to assign confidence levels to annotations.</li><li>Using visualization and statistical tools to interpret and compare complex datasets.</li></ol><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Thermo Scientific Compound Discoverer 3.5 software </a>brings together all the components needed for robust PFAS identification—integrating <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=22&amp;manufacturerItems=3">HRAM Orbitrap MS</a> data with an expanded suite of PFAS-specific libraries and databases.In a single workflow, users can:<ul><li>Detect and annotate compounds automatically from LC-MS data.</li><li>Determine possible elemental formulas using isotope pattern and accurate mass information.</li><li>Compare MS² spectra against <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1764">mzCloud</a>, NIST 2023, and in silico PFAS libraries.</li><li>Cross-reference annotations with the EPA PFAS Structure List and the NIST Suspect List of PFAS.</li><li>Use PFAS-specific fragment and neutral loss databases to strengthen identification confidence.</li></ul>With these resources combined, the software enables confident annotation of more than 40,000 PFAS compounds!<h3>New tools for confident, streamlined PFAS identification</h3>Compound Discoverer software introduces several new features designed to simplify and accelerate PFAS NTA workflows:<ul><li>Apply PFAS Scoring Node: Automatically calculates PFAS-specific parameters and a new PFAS Score—no R scripting required.</li><li>PFAS Plot: A visual tool that uses mass-defect metrics to highlight probable PFAS in complex datasets, following methods from Kaufman et al. (2022).</li><li>Expanded suite of libraries and databases: Combines mzCloud, NIST, in silico, PFAS fragment databases, and a new neutral loss database for greater confidence.</li><li>Advanced data visualization: Tools like Kendrick Mass Defect and Molecular Network plots reveal homologous PFAS series and related compound families.</li><li>Built-in statistical analysis tools: Compare PFAS composition between samples using Principal Component Analysis, Differential analysis and Hierarchical Cluster Analysis.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg" alt="Thermo Fisher Scientific From Complexity to Clarity Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg 245w," sizes="100vw" width="800" height="395"><h3>From complexity to clarity</h3>By uniting Orbitrap HRAM data with the most comprehensive PFAS libraries available, Compound Discoverer 3.5 software streamlines non-targeted PFAS analysis—helping laboratories move from data complexity to confident chemical clarity.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5612">Watch the on-demand webinar</a> to see the full workflow in action and learn how Compound Discoverer software can enhance your PFAS analysis.<ul><li>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#PFAS #CompoundDiscoverer #SpectralLibraries</li></ul><blockquote><h5>Zoe Julian</h5>Zoe Julian is a Product Marketing Manager for Chromatography and Mass Spectrometry software at Thermo Fisher Scientific. With over 12 years' experience of working with chromatography and mass spectrometry techniques with a background in GC and GC-MS(/MS) organic environmental analysis, she has helped many laboratories and industries improve their workflows through a unique understanding of their challenges. Transitioning to a focus on Chromeleon CDS she has shown customers how software solutions can improve their productivity and support their day-to-day operations.</blockquote>

From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software

<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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UV-Vis measurements you want to make do not always fall into the realm of a liquid in a standard cuvette.
When this is the case, the ability to add an accessory to the sample compartment or in the case of the Cary 3500, swap out a sampling Module, is key to increasing your measurement capabilities. This presentation will explore various options for expanding measurement capabilities, especially the Cary 3500 with the recently introduced Flexible Module.
<blockquote>
Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 35 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
</blockquote>

agilent Technologies: Expanding Your UV-Vis Measurement Capabilities through the use of Accessories

Expanding Your UV-Vis Measurement Capabilities through the use of Accessories

Explore various options for expanding measurement capabilities, especially the Cary 3500 with the recently introduced Flexible Module.

Expanding Your UV-Vis Measurement Capabilities

Ion Mobility (IM) advancements in Software, Data Bases, AI and Machine learning are quickly improving capabilities to make unknown identifications, separate and ID isomers, as well as improve workflows associated with metabolomics, lipidomics, PFAS, and qual-quant screening.
In addition, IM measurements are significantly improved with HRdm High Resolution Demultiplexing. This is the first commercial IM instrument to enable the simultaneous combination of high drift resolution, while at the same time achieving all four of the following measurement capabilities: broad mass range in a single injection, multiplexing to increase sensitivity, compatibility with fast chromatographic acquisition rates , and highest precision in CCS measurements. For more accurate CCS predictions, the better precision in the CCS training set improves AI machine learning SW. This can significantly reduce the number of potential candidate structures to select from when making unknown identifications.
<blockquote>
Presenter: Brock Juliano (Ph.D. student, Brandon Ruotolo lab, Dept of Chemistry, Univ of Michigan)
</blockquote>

Agilent Technologies: Development of RapidFire Native Mass Spectrometry and Collision Induced Unfolding Methodologies for High Throughput Screening of Protein and Oligonucleotide Biotherapeutics.

Development of RapidFire Native Mass Spectrometry and Collision Induced Unfolding Methodologies for High Throughput Screening of Protein and Oligonucleotide Biotherapeutics.

Development of RapidFire Native Mass Spectrometry and Collision Induced Unfolding Methodologies for High Throughput Screening.

RapidFire Native MS of Proteins and Oligonucleotides

In modern pharmaceutical analysis, the utilization of Pharmacopeia regulated U/HPLC (ultra/high-performance liquid chromatography) methods has become a fundamental aspect of laboratory operations.
This webinar aims to explore the diverse strategies and techniques that can be employed to fully leverage the potential of U/HPLC method adaptation, thereby enhancing the effectiveness and efficiency of laboratory processes. The presentation delves into the principles of U/HPLC and highlights the significance of adhering to Pharmacopeia regulations for ensuring reliable and accurate analytical results. Furthermore, various approaches for method adaptation, such as modification of mobile phase composition, adjustment of column parameters, and optimization of detection conditions will be discussed, including the benefits and challenges associated with each adaptation strategy and insights into the factors influencing method transfer and development.
Additionally, the potential applications of U/HPLC method adaptation in pharmaceutical quality control, impurity profiling, and stability testing will be explored. By understanding the nuances and intricacies of U/HPLC method adaptation, laboratories can unlock new avenues for improving productivity, reducing costs, and enhancing the overall quality of pharmaceutical analysis.
By attending this presentation you will:
<ul>
<li>Learn about the principles and importance of U/HPLC method adaptation in pharmaceutical analysis and how improved methods can help to boost laboratory effectiveness and efficiency
</li>
<li>Gain insights into the significance of adhering to Pharmacopeia regulations for ensuring reliable and accurate analytical results
</li>
<li>Understand various strategies and techniques for adapting U/HPLC methods, such as modifying mobile phase composition, and adjusting column parameters
</li>
<li>Leverage the full potential of U/HPLC method adaptation leading to improved productivity and cost reduction in the laboratory
</li>
</ul>
Who should attend
<ul>
<li>Lab managers and technicians working with HPLC/UHPLC methods (R&amp;D, QC and method transfer).</li>
</ul>
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Presenter: 
</blockquote>
<blockquote>
Presenter: Petra Lewits (Global Product Manager for Analytical Chromatography, HPLC columns, Merck KGaA, Darmstadt, Germany)
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Petra Lewits has worked with chromatography for 40 years. In 1986 she joined the pharmaceutical R&amp;D and QC department (Merck KGaA, Darmstadt, Germany) where she developed HPLC methods for R&amp;D and QC of pharmaceutical APIs and formulations as well as for diet convenience foods. Since 2002 she has been a Global Product Manager for Analytical Chromatography (HPLC). In 2014 she began focusing on thin-layer chromatography (TLC). Since April 2018 Petra has had global responsibility for analytical HPLC columns.
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<blockquote>
Presenter: Egidijus Machtejevas (Senior Technical Advisor, Analytical Chromatography Workflows, Merck KGaA, Darmstadt, Germany)
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Egidijus Machtejevas joined the R&amp;D Department at Merck KGaA, Darmstadt, Germany in 2008. A year later he became marketing manager for North America, and in 2010–2018 Product Manager for chromatography. Currently he is a Senior Technical Advisor in the Analytical Chromatography Workflows group. Egidijus has twenty-five scientific papers and thirteen book chapters to his name, and his major focus areas include chromatography, multidimensional liquid chromatography, proteomics and mass spectrometry.
</blockquote>

Pixabay/Ri Butov: Leverage the full potential of Pharmacopeia regulated U/HPLC method adaptation to increase lab effectiveness

Leverage the full potential of Pharmacopeia regulated U/HPLC method adaptation to increase lab effectiveness

Explore the diverse strategies and techniques that can be employed to fully leverage the potential of U/HPLC method adaptation.

Leverage the full potential of Pharmacopeia

Key Learning Points:
<ul>
<li>Receive an overview of theory of HPLC mobile phase/solid phase and their interactions 
</li>
<li>Learn the differences between mobile phase options, and what to consider when picking a mobile phase 
</li>
<li>Review the of panel results and how mobile phases affected ionization and separation
</li>
</ul>
Who Should Attend:
<ul>
<li>Any HPLC and LC-MS/MS scientists in clinical and toxicology looking to learn more about the theory of HPLC mobile phase</li>
</ul>
<blockquote>
Presenter: Mackenzie Freige (Application Scientist - Clinical, Phenomenex)
</blockquote>
<blockquote>
Mackenzie is an Application Scientist at PhenoLogix, and is based in Torrance, CA. She has undergraduate degrees in Chemistry and in Life Sciences. She joined Phenomenex in 2021, and brings extensive experience in liquid chromatography-mass spectrometry. In her free time she practices yoga and enjoys reading. 
</blockquote>

Phenomenex: HPLC Method Development – Choosing a Mobile Phase for Your Assay

HPLC Method Development – Choosing a Mobile Phase for Your Assay

Receive an overview of theory of HPLC mobile phase/solid phase and their interactions.

HPLC Method Development – Choosing a Mobile Phase

This popular online event will once again bring together leading scientists in the field as they highlight the latest advancements in translational proteomics and metabolomics. Presentations will cover areas ranging from uncovering the molecular mechanisms of disease pathology, to the latest technological developments in diagnostics and treatment.
This online symposium will explore:
<ul>
<li>Cutting-edge proteomics for brain aging and dementia biomarkers</li>
<li>Innovations in single-cell proteomics technology</li>
<li>Next-gen protein sequencing and PTM detection</li>
<li>Healthcare transformation through data analytics and remote monitoring</li>
</ul>
<h3>Agenda</h3>
<h5>16:00 – 16:40 Deciphering Microbial Omics Signatures in Multidimensional Mass Spectrometry</h5>
<ul>
<li>Speaker: Aivett Bilbao, PhD</li>
</ul>
<h5>16:40 – 16:50 Networking Break</h5>
<h5>16:50 – 17:10 Precision Proteomics Accelerates the Early Detection of Dementia</h5>
<ul>
<li>Speaker: Mariana Fontes, PhD</li>
</ul>
<h5>17:10 – 17:20 Networking Break</h5>
<h5>17:20 – 18:10 Towards the Making of Molecular Movies Directed by the Circulating Proteome</h5>
<ul>
<li>Speaker: Prof. Jochen Schwenk</li>
</ul>
<h5>18:10 – 18:20 Networking Break</h5>
<h5>18:20 – 18:40 Lipidomic Variabilities of Human Blood Plasma</h5>
<ul>
<li>Speaker: Prof. Markus Wenk</li>
</ul>
<h5>18:40 – 18:50 Networking Break</h5>
<h5>18:50 – 19:30 The SEISMIC Facility for Spatially Resolved Single and Sub-Cellular Omics</h5>
<ul>
<li>Speaker: Prof. Melanie Bailey</li>
</ul>

Technology Networks: Advances in Proteomics & Metabolomics 2023

Advances in Proteomics & Metabolomics 2023 - Day 2

Areas ranging from uncovering the molecular mechanisms of disease pathology, to the latest technological developments in diagnostics and treatment.

This presentation will provide an overview of analytical quality by design (AQbD) principles and the evolution of QbD in the pharmaceutical development environment.
A brief introduction to the new USP chapter &lt;1220&gt; Analytical Procedures Life Cycle will also be given, including a short case study on stage 1 — procedure design. Additionally, key aspects of the new ICH Q14/Q2(R2) draft guidelines will be discussed along with their implications.
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Presenter: Amanda Guiraldelli, PhD (Scientific Affairs Manager, U.S. Pharmacopeia)
</blockquote>
<blockquote>
Amanda Guiraldelli has been with USP since 2012 and holds the position of scientific affairs manager and principal scientist in the compendial science group-general chapters. She also serves as scientific liaison for the USP Measurement and Data Quality Expert Committee, where she works to develop and revise USP standards related to analytical procedure lifecycle/validation.
</blockquote>
<blockquote>
Previously, Amanda worked as senior scientist at the USP reference standard laboratory for 8 years with characterization of compendial standards. She is visiting professor at the Institute of Chemistry at the University of Campinas (UNICAMP) in Brazil and is a frequent speaker and instructor on topics related to analytical procedure life cycle, analytical quality by design (AQbD) and chromatography. Amanda is a specialist in mass spectrometry, chromatography, omics, and chemometrics and has more than 15 years of experience in pharmaceutical R&amp;D areas.
</blockquote>
<blockquote>
Presenter: Manu Grover (M.S. (Pharm), Business Development Manager, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Manu has more than 21 years of pharmaceutical industry and chromatography experience. He has worked in the fields of manufacturing and analytical research to support IND, NDA, and ANDA filings in pharmaceutical companies like Dr. Reddy&#39;s, Panacea Biotec, and Ozone Pharmaceuticals. In the course of his professional journey, Manu has developed and validated many analytical methods on HPLC, GC, particle sizing, DSC, pXRD, LC/MS, and more. He has successfully filed multiple analytical dossiers in the US and EU markets, filed analytical methods to USP, and handled multiple US FDA audits.
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Agilent Technologies: Introduction to USP 1220 Analytical Procedures Life Cycle and Implications of ICH Q14 and Q2(R2) Guidelines

Introduction to USP 1220 Analytical Procedures Life Cycle and Implications of ICH Q14 and Q2(R2) Guidelines

Analytical quality by design (AQbD) principles and the evolution of QbD in the pharmaceutical development environment.

Introduction to USP 1220 Analytical Procedures Life Cycle

In this on-demand webinar, Wai-Chi Man, Product Marketing Manager at Thermo Fisher Scientific, discussed the basics of why Ion Chromatography coupled with Mass Spectrometry (IC-MS) can be an added benefit to any High-Performance Liquid Chromatography with Mass Spectrometry (LC-MS), and complimentary to any Hydrophilic Interaction Liquid Chromatography (HILIC) analysis.
You can also expect to:
<ul>
<li>Enhance your understanding of the instrument requirements for IC and MS: which one is best for your application of interest.
</li>
<li>Learn how to set up the connectivity or simply switch from IC-MS to LC-MS.
</li>
<li>Explore the switch over from applications such as Polar Pesticides from Anionic to Cationic with one instrument set-up, and how it’s applicable to other applications such as Metabolomics.
</li>
</ul>
<blockquote>
Presenter: Wai-chi Man (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Wai-Chi Man, MRSC has been with Thermo Fisher Scientific since 2011. She started on the application sales support team but is now a product marketing manager. She has specialized in ion chromatography (IC) for more than 33 years, with over 36 years of experience in analytical chemistry in a wide variety of techniques. Before Thermo Fisher Scientific, she spent seven years at Wellcome Research supporting structural elucidation for medicinal chemists using a wide range of analytical instrumentation. Wai-Chi spent a total of 20 years in the pharma industry as part of GlaxoSmith Kline, from research to transferring methods to manufacturing. She worked on and was responsible for many drugs from anti-viral to respiratory in the market today.
</blockquote>
<blockquote>
Wai-Chi’s work at Thermo Fisher Scientific has supported a wide range of customers from various industries, providing IC support in method development, troubleshooting, sample preparation, trainings, seminars, events, and workshops. This experience has helped her build greater knowledge, skill and understanding within areas such as IC-MS, IC-ICP-MS, HPAE-PAD analysis, metabolomics, and polar pesticides.
</blockquote>

Thermo Scientific: Back to Basics – IC-MS 

Back to Basics – IC-MS 

The basics of why Ion Chromatography coupled with Mass Spectrometry (IC-MS) can be an added benefit to any High-Performance Liquid Chromatography.

There are GxP regulations specifically relating to the controls required for the use of electronic records, electronic signatures, and computerized systems. These regulations require a combination of procedural, technical, and behavioral controls to achieve full and ongoing compliance.
It is important for Quality Units and regulators to understand the capabilities available for processing data and, the need for varied levels of flexibility within the Empower Software Solutions based on the chromatography and types of molecules being analyzed. There can be a perception that analysts may use Processing Method parameters or manual integration to introduce human bias, or even “polish results” in a way that would cause otherwise out-of-specification (OOS) samples to pass laboratory test requirements such as assays and impurity methods. To fully ascertain what is happening in the laboratory, both the Quality Unit and regulators should be fully aware of how an organization utilizes the Review window to optimize Processing Method parameters.
In this webinar we will examine the current system policies and privileges in Empower Software that affect what analysts can and cannot do when processing data, particularly in Review.
Please join us for a live discussion. As always, don’t forget to bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.
<blockquote>
Presenter: Neil Lander (Principal Product Manager - Informatics Group, Waters Corporation)
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<blockquote>
Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager in Waters in the Informatics group. Neil provides internal support for Empower CDS software and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters Corporation, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.
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<blockquote>
Presenter: Nisar Ahmed (Empower Applications Consultant, Waters Corporation)
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<blockquote>
Nisar joined Waters Corporation in 1998 and has held a variety of technical positions including Technical Support Specialist, Customer Education Manager and currently Empower Applications Consultant. Nisar works with Waters as an Informatics Consultant. His current role is to act as a resource across Europe providing support, consultancy and training to laboratories implementing and/or using Empower Software as their CDS in a compliant and regulated environment. Nisar joined Waters after a career in the pharmaceutical industry as an analytical chemist. He also holds a Master of Science degree from the University of London.
</blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Configuring Empower Software to Maximize Your Compliance

Empower Tips: Ask Me Anything About Configuring Empower Software to Maximize Your Compliance

Current system policies and privileges in Empower Software that affect what analysts can and cannot do when processing data, particularly in Review.

Ask Me Anything About Configuring Empower Software

Christian Rohrer, Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.
Join Marissa Heino, Product Specialist MCC, and Sebastian Thürmann, Product Manager MCC, as they dive into the process of transitioning from an established batch capture step to a multi-column chromatography (MCC) method with ProComposer MethodWizard software and MCC specific pre-packed columns to be run on Octave BIO. They will discuss scale-up using the ProComposer software and explore its integration with the newly launched GMP-ready skid, the Octave PRO, with GMP ready pre-packed columns. Finally, they will provide insight into the potential this technology has to enhancing workflow.
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Presenter: Christian Rohrer (Director of Sales &amp; Support – EMEA)
</blockquote>
<blockquote>
Presenter: Marissa Heino (Product Specialist MCC)
</blockquote>
<blockquote>
Presenter: Sebastian Thürmann (Product Manager MCC)
</blockquote>

Tosoh Bioscience: Multi-Column Chromatography Scale-Up with the Octave Platform

Multi-Column Chromatography Scale-Up with the Octave Platform

Process of transitioning from an established batch capture step to a multi-column chromatography (MCC) method.

Multi-Column Chromatography Scale-Up with Octave Platform

Mass Spectrometry Imaging (MSI) is a technique that allows chemical information to be obtained directly or via the use of matrices from a wide range of surfaces. MS Imaging provides information on the spatial distribution of molecules at or near the surface. Imaging provides beautiful and information-rich images that allow scientists to answer the fundamental question: “Where is this component in my sample?”
In this webinar series, we will examine the basics of MS imaging, compare the techniques of untargeted and targeted MS Imaging and the information that can be gained from each, and end with an interview from Dr. Ryan Leib (Stanford University) and Dr. Roy Martin (Waters Corporation) on the practicalities of setting up a MS Imaging Core lab.
Who Should Attend: Any scientist, lab managers or lab directors looking to expand (or refresh) their knowledge of MS Imaging.
<h3>Part Two – October 17th, 2023: Untargeted versus Targeted Imaging</h3>
In the second session, we will look at the differences between untargeted and targeted imaging: the systems on which the data can be acquired, what data is collected using each technique, how data can be analyzed and how they can work together to provide a complete picture.
<blockquote>
Presenter: Anthony Midley (Principal Applications Support Scientist, Waters Corporation)
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<blockquote>
Anthony Midey is a Principal Applications Support Scientist in Waters Biomedical Research group at Americas Application Laboratory, providing pre- and post-sales support in DESI, MALDI, and imaging mass spectrometry. He is also the BMR team lead for targeted metabolomics and lipidomics LC-MSMS applications. Anthony received his Ph.D. in spectroscopy and mass spectrometry from the University of Rochester and joined Waters in 2015. He has over 55 publications in peer-reviewed journals, with over 10 years working in the analytical instruments sector.
</blockquote>
<blockquote>
HOST: Kieron Faherty, Ph.D. (Separations and Mass Spectrometry Field Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Kieron Faherty is the Separations and Mass Spectrometry Field Marketing Manager for the Americas at Waters Corporation. He received his B.Sc. (Hons) in Chemistry from the University of Reading (Reading, Berkshire, UK) and his doctorate in Chemistry from the University of Massachusetts (Amherst, MA). He has held several field-based positions at Waters, most recently as a Field Applications Specialist focused on biopharmaceutical applications using Time-of-Flight mass spectrometry instrumentation.
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