Per- and polyfluoroalkyl substances (PFAS) have become a major global concern due to their persistence, bioaccumulation, and potential health impacts. With thousands of known PFAS compounds, and new variants continuing to emerge, identifying and quantifying them across complex environmental and biological samples is an ongoing analytical challenge.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg" alt="Thermo Fisher Scientific: From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg 245w," sizes="100vw" width="1024" height="574"><h3>Why PFAS analysis matters</h3>PFAS are used in everything from firefighting foams to non-stick coatings, yet their chemical stability means they persist in the environment and accumulate in living organisms. Regulators and scientists around the world are racing to better understand their distribution, fate, and toxicity.But with tens of thousands of PFAS, many without available reference standards, traditional targeted analysis alone isn’t enough. Analysts increasingly rely on non-targeted analysis (NTA) workflows powered by high-resolution accurate-mass (HRAM) mass spectrometry and advanced software to uncover the full picture.<h3>Addressing the challenges of PFAS identification</h3>Successful non-targeted PFAS analysis requires overcoming several analytical hurdles:<ol><li>Acquiring high-quality MS¹ and MS² data with ultra-high mass resolution and accuracy.</li><li>Detecting low-abundance PFAS hidden within thousands of background compounds.</li><li>Leveraging comprehensive spectral libraries and databases for confident identification.</li><li>Applying data filtering and scoring tools to assign confidence levels to annotations.</li><li>Using visualization and statistical tools to interpret and compare complex datasets.</li></ol><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Thermo Scientific Compound Discoverer 3.5 software </a>brings together all the components needed for robust PFAS identification—integrating <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=22&amp;manufacturerItems=3">HRAM Orbitrap MS</a> data with an expanded suite of PFAS-specific libraries and databases.In a single workflow, users can:<ul><li>Detect and annotate compounds automatically from LC-MS data.</li><li>Determine possible elemental formulas using isotope pattern and accurate mass information.</li><li>Compare MS² spectra against <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1764">mzCloud</a>, NIST 2023, and in silico PFAS libraries.</li><li>Cross-reference annotations with the EPA PFAS Structure List and the NIST Suspect List of PFAS.</li><li>Use PFAS-specific fragment and neutral loss databases to strengthen identification confidence.</li></ul>With these resources combined, the software enables confident annotation of more than 40,000 PFAS compounds!<h3>New tools for confident, streamlined PFAS identification</h3>Compound Discoverer software introduces several new features designed to simplify and accelerate PFAS NTA workflows:<ul><li>Apply PFAS Scoring Node: Automatically calculates PFAS-specific parameters and a new PFAS Score—no R scripting required.</li><li>PFAS Plot: A visual tool that uses mass-defect metrics to highlight probable PFAS in complex datasets, following methods from Kaufman et al. (2022).</li><li>Expanded suite of libraries and databases: Combines mzCloud, NIST, in silico, PFAS fragment databases, and a new neutral loss database for greater confidence.</li><li>Advanced data visualization: Tools like Kendrick Mass Defect and Molecular Network plots reveal homologous PFAS series and related compound families.</li><li>Built-in statistical analysis tools: Compare PFAS composition between samples using Principal Component Analysis, Differential analysis and Hierarchical Cluster Analysis.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg" alt="Thermo Fisher Scientific From Complexity to Clarity Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg 245w," sizes="100vw" width="800" height="395"><h3>From complexity to clarity</h3>By uniting Orbitrap HRAM data with the most comprehensive PFAS libraries available, Compound Discoverer 3.5 software streamlines non-targeted PFAS analysis—helping laboratories move from data complexity to confident chemical clarity.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5612">Watch the on-demand webinar</a> to see the full workflow in action and learn how Compound Discoverer software can enhance your PFAS analysis.<ul><li>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#PFAS #CompoundDiscoverer #SpectralLibraries</li></ul><blockquote><h5>Zoe Julian</h5>Zoe Julian is a Product Marketing Manager for Chromatography and Mass Spectrometry software at Thermo Fisher Scientific. With over 12 years' experience of working with chromatography and mass spectrometry techniques with a background in GC and GC-MS(/MS) organic environmental analysis, she has helped many laboratories and industries improve their workflows through a unique understanding of their challenges. Transitioning to a focus on Chromeleon CDS she has shown customers how software solutions can improve their productivity and support their day-to-day operations.</blockquote>

From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software

<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

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Article

Product

Application

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Video

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

Shimadzu Corporation

Waters Corporation

Having good peak shape can improve resolution and produce accurate and reliable quantitation results. Problems with peak shape, like fronting, tailing, shoulders, and splits, are all indicative of chromatography issues.
We will discuss factors affecting peak shape, such as the column, sample, mobile phase, and more, and offer remedies for improving peak shape.
<blockquote>
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
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<blockquote>
Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.
</blockquote>
<blockquote>
Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.
</blockquote>
<blockquote>
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
</blockquote>

Peak Shape and Why It Matters: How to Get Good Peak Shape

We will discuss factors affecting peak shape, such as the column, sample, mobile phase, and more, and offer remedies for improving peak shape.

Do you wish to quickly uncover the best nanoparticle formulation, and achieve accurate and reliable results in any dissolution tests for tablets or liposome drug products?
The Agilent NanoDis system provides formulation scientists with accurate release profiles of APIs using conventional dissolution test apparatus in an automated, compliant manner.
Attend this The Lab Central premiere to know more about our advanced solution for Nanoformulation release testing.
<blockquote>
Presenter: Mr. Nitin G (Product Specialist Dissolution, Agilent Technologies)
</blockquote>

Agilent Technologies: Nanoformulation release testing: Explained

Nanoformulation release testing: Explained

Uncover the best nanoparticle formulation, and achieve accurate and reliable results in any dissolution tests for tablets or liposome drug products?

Per- and polyfluoroalkyl substances (PFAS) are synthetic compounds that have been the focus of research development for targeted quantitation.
PFAS are present in a variety of consumer products as well as firefighting foam that collectively can be introduced into the environment. Due to the chemical structure, these compounds are hard to break down and are therefore labeled as forever chemicals. As such, once PFAS compounds have been introduced into the environment, they can leach from their origin into the environment and have been detected in surface waters, soils, dust, plant tissue, and even in the air. Recently, concerns have escalated not only for understanding the wider environmental impact, but animal and human health have evolved as research has now focused on detecting and quantifying in human plasma and tissue.
The focus of this webinar is to explore the range of sample types examined for routine PFAS detection and quantification. Dr. John Bowden will provide an overview of the challenges non-water matrices presents for extracting and quantifying including finding blanks and validating the workflows. In addition, research will cover new methods to introduce a wide range of PFAS onto UHPLC columns and perform low-level detection and quantification of PFAS in human plasma by LC-MS/MS. The second part of the webinar will focus on targeted PFAS quantitation using the Thermo Scientific™ TSQ Quantis™ Plus mass spectrometers supporting the EPA draft method 1633 which includes sample preparation using solid phase extraction (SPE) prior to LC-MS/MS analysis. The presented workflow will demonstrate key experimental strategies to improve laboratory productivity demonstrating not only effective sample preparation options but the software solutions critical to streamline the data processing to create standard reports.
Key learning objectives: 
<ul>
<li>Evaluation of the PFAS kits on the Thermo Scientific™ Vanquish™ UHPLC systems to minimize background interference to achieve stated LOQs
</li>
<li>Review of new hardware and software components in the Thermo Scientific™ TSQ Plus mass spectrometers to boost PFAS analysis
</li>
<li>Demonstration of Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) software designed to manage the entire experiment and provide comprehensive reports to increase laboratory productivity
</li>
</ul>
Who should attend?
<ul>
<li>Laboratories that are contracted for PFAS testing
</li>
<li>Researchers looking for workflows to increase detection and quantitation limits
</li>
<li>Regulatory agencies that are focused on developing new testing methods and requirements for PFAS studies
</li>
<li>Existing Thermo Scientific™ TSQ and Thermo Scientific™ Orbitrap™ mass spectrometer operators who are interested in the next-level performance and productivity with the TSQ Plus mass spectrometer platforms
</li>
</ul>
<blockquote>
Presenter: Dr. John A. Bowden (Assistant Professor, University of Florida, (Gainesville))
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Dr. Bowden received his B.S. in Chemistry in 2003 and in 2008 received his Ph.D. in Analytical Chemistry both from the University of Florida. John was a Postdoctoral Fellow in the Dept. of Biochemistry and Molecular Biology at St. Louis University from 2009-2011, and then a Postdoctoral Fellow with the National Institute of Standards and Technology in Charleston, S.C. from 2011-2013 and then became a Research Chemist from 2013-2018. In 2018, John joined the University of Florida and has published over 60 journal articles and over 20 focusing on PFAS.
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Presenter: Kevin J. McHale, Ph.D. (Senior LC-MS Application Scientist, Thermo Fisher Scientific)
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Kevin is a Senior LC-MS Applications Scientist with Thermo Fisher Scientific. Starting his professional career in 1994 with DuPont Merck, Kevin helped implement “walk-up” LC-MS systems supporting synthetic medicinal chemists. In 1997, Kevin left to pursue his Ph.D. in Analytical Chemistry under the direction of Professor Rick Yost at the University of Florida. Kevin’s research focus was studying ion trap fundamentals and developing nano LC-MS/MS techniques. Upon completion in 2002, Kevin joined Thermo Fisher Scientific as an LC-MS Marketing Specialist supporting the Thermo Scientific™ TSQ Quantum™ triple quadrupole mass spectrometer. With over 21 years at Thermo Fisher, Kevin presently specializes in developing methodology for quantitative LC-MS analyses of small organic compounds on the Thermo Scientific™ TSQ and Thermo Scientific™ Orbitrap Exploris™ mass spectrometer product lines.
</blockquote>

Thermo Scientific: Expanding the scope of PFAS screening and quantitation through targeted acquisition – water analysis and beyond

Expanding the scope of PFAS screening and quantitation through targeted acquisition – water analysis and beyond

Explore the range of sample types examined for routine PFAS detection and quantification.

Expanding the scope of PFAS screening and quantitation

<h5>Solving Problems that Matter: Risk Mitigation in the Pharma QC Laboratory</h5>
Expert Insights 3-Part Webinar Series: Risk Assessment, Control Strategies, and Intuitive Innovation to Improve Data Quality, Data Integrity and Lab Productivity. 
Pharma QC labs are facing increasing pressure on the cost of quality while maintaining compliance and assuring patient safety. From the harmonization of USP &lt;621&gt; to Empower™ workflows supporting data integrity to improving method transfer outcomes, you will learn from subject matter experts how to reduce errors and mitigate risk for reproducible data and enhanced productivity. 
Who should attend
<ul>
<li>Pharma QC Lab directors, managers and senior scientists with a drive to reduce errors, improve lab productivity and enhance data quality &amp; integrity.</li>
</ul>
<h3>PART 2 l Improving Method Transfer Outcomes with Intuitive Innovation</h3>
<ul>
<li>Wednesday, August 16th, 11:30-12:30 ET</li>
</ul>
Your LC systems need to support both legacy and new methods that can be operated repeatably anywhere in the world while meeting regulatory requirements. These requirements can also place a large burden on your analysts, but failing to perform necessary tasks could put your organization at risk.
This webinar will introduce our latest HPLC innovation – the Alliance iS HPLC System – that features improved enhanced temperature control to match global needs. Additional features include our intelligent method translator app (iMTA), method matching app, system audit trail and automated column history tracking.
<blockquote>
Presenter: Lise Gauthier (Principal Scientist, Waters Corproation)
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<blockquote>
Lise Gauthier is Principal Scientist within the QA/QC Business Strategy group at Waters Corporation in Milford, MA. Prior to joining Waters in 2014, Lise worked as a scientist as Astra Zeneca for 20 years holding positions of increasing responsibility within Quality Assurance and Drug Discovery. Her current role at Waters includes system validation and evaluation of competitive systems.
</blockquote>

Waters Corporation:  Improving Method Transfer Outcomes with Intuitive Innovation

 Improving Method Transfer Outcomes with Intuitive Innovation

Latest HPLC innovation – the Alliance iS HPLC System – that features improved enhanced temperature control to match global needs.

Method Transfer Outcomes with Intuitive Innovation

Dive into the world of biopharma analysis with us as we cover the essential aspects of oligonucleotides, a group of rapidly advancing drugs that offers promising treatments and holds great potential for personalized medicines.
We will showcase state-of-the-art solutions that support the entire workflow employed by Contract Drug Manufacturing Organizations (CDMOs), from R&amp;D to validation and manufacturing. Get valuable insights into biopharmaceutical drug development and delve into the capabilities of our solutions, including our Nexera XS inert system, LCMS-9050 QTOF, and the latest LabSolutions Insight Biologics software.
Learning Objectives:
<ul>
<li>Introduction to the crucial aspects of oligonucleotides as therapeutics
</li>
<li>Understand the increasingly important role of LCMS in biopharma for manufacturing, QC, and confirmation
</li>
<li>Explore Shimadzu’s latest hardware and software solutions
</li>
</ul>
<blockquote>
Presenter: Neil Loftus (General Manager, Mass Spectrometry Business Unit (Overseas), Shimadzu Corporation)
</blockquote>
<blockquote>
Neil Loftus is an expert in developing new mass spectrometry technologies and innovative data processing workflows to solve complex analytical problems. As the General Manager of the Mass Spectrometry Business Unit (Overseas) at Shimadzu Corporation, he brings a wealth of experience to his current role. Neil&#39;s professional journey began at the London Hospital Medical College, where he focused on academic and pharmaceutical research applications in metabolism and pharmacokinetics. He then continued his work in clinical quantitative bioanalysis and metabolite identification in an industrial setting before transitioning into mass spectrometry. Throughout Neil’s career, he has remained deeply involved in mass spectrometry research and development. His contributions include publishing scientific papers on pharmaceutical metabolite identification and biomarker research, as well as generating new solutions to change the way we think about data and solve problems that matter.
</blockquote>
<blockquote>
Presenter: Max Kosok (Senior Product Specialist (LCMS), Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
With more than 14 years of experience, Dr. Kosok is a passionate expert in mass spectrometry. He has received is PhD from National University of Singapore in Biochemistry doing proteomics research on triple negative breast cancer by high resolution LCMS. In 2020, he joined Shimadzu to deliver end-to-end LCMS application solutions for customers. In his current role as a Product Specialist for LCMS and previously as an application chemist, Dr. Kosok has worked with various pharma and biopharma customers. He has supported a multitude of applications from impurity analysis to biomolecule characterization, particularly on Shimadzu’s LCMS QTOF technology.
</blockquote>

Shimadzu: Towards the Future of Biopharma: Insights into Oligonucleotide Therapeutics

Towards the Future of Biopharma: Insights into Oligonucleotide Therapeutics

State-of-the-art solutions that support the entire workflow employed by Contract Drug Manufacturing Organizations (CDMOs).

Insights into Oligonucleotide Therapeutics

The USP general chapter &lt;1220&gt; &quot;Analytical Procedure Life Cycle&quot; became official in May 2022. The adoption of this guideline makes your lab ready for the adoption of the ICH Q14 “Analytical Procedure Development” and Q2(R2) “Analytical Validation”, expected later in 2023.
These quality guidelines are relevant for both analytical development and QC testing at pharma companies. Empower and Waters solutions support all 3 steps of the guideline, namely: 
<ul>
<li>Development
</li>
<li>Validation
</li>
<li>On-going Verification
</li>
</ul>
In Part I we will describe how Empower supports Step 1 – Development through the integration with selected QbD software that enables the required DoE approach.
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Presnter: Andrea Gheduzzi (Principal Pharma Development Specialist)
</blockquote>

Waters Corporation: USP <1220> and ICH Q14/Q2(R2) using Empower / Part I - Development

USP <1220> and ICH Q14/Q2(R2) using Empower / Part I - Development

Development through the integration with selected QbD software that enables the required DoE approach.

USP <1220> and ICH Q14/Q2(R2) using Empower - Development

This series is comprised of four different, dissolution focused, lightning talks. Sessions have been optimized to give you the information you need in a flash - less than 30 minutes per session. Topics include tips for preventing failures, improving efficiency, and more!
This presentation covers the basics of adapting the dissolution environment for low-dose, extended-release dosage forms (e.g., medical devices).
<blockquote>
Presenter: Ken Boda (Dissolution Product Specialist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Ken joined Varian (now Agilent) in 2005 as an Applications Engineer, is now a Product Specialist. Prior to working with Agilent, he worked in pharma specialized in dissolution testing and method development. He works with customers in determining the best systems for their needs and optimizing their work flows. He has also helped educate customers on dissolution science through webinars, the Dissolution Discussion Group, LinkedIn, etc.
</blockquote>

Agilent Technologies: A How-To Guide for Release Rate Testing of Medical Devices

A How-To Guide for Release Rate Testing of Medical Devices

This presentation covers the basics of adapting the dissolution environment for low-dose, extended-release dosage forms (e.g., medical devices).

While making a UV-Vis measurement is simple, making an accurate measurement requires an understanding of instrument parameters and their effects on the measurement. Parameters include Baseline correction, spectral bandwidth, data interval, and many additional settings.
This presentation will provide an insight into these parameters and more.
<blockquote>
Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 35 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
</blockquote>
<blockquote>
Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
</blockquote>

Agilent Technologies: Understanding UV-Vis Instrumental Parameters

Understanding UV-Vis Instrumental Parameters

This presentation will provide an insight into baseline correction, spectral bandwidth, data interval, and many additional settings

In this work, we present novel, automated, and integrated software to support these workflows using LC HRAM MS instruments.
Purity and sequencing data are presented for a multitude of oligos including antisense, aptamer, and long synthetic samples.
<blockquote>
Presenter: Shashank Jain, PhD (Mass Spec product specialist, New England, Agilent Technologies, Inc.)
</blockquote>

Agilent Technologies: Relieve the Pressure of Oligo Characterization by Implementing Automated Workflows

Relieve the Pressure of Oligo Characterization by Implementing Automated Workflows

Novel, automated, and integrated software to support these workflows using LC HRAM MS instruments.

Oligo Characterization by Implementing Automated Workflows

Are you using Chromeleon CDS but don’t feel like an expert just yet? Are you interested in a CDS that is intuitive and user-friendly? Whether you’re a day-to-day user or just curious about a new CDS, this is the webinar for you!
What this session covers:
<ul>
<li>Fundamentals of Chromeleon CDS</li>
<li>How to create custom reports</li>
<li>New features in Chromeleon 7.3.2 CDS</li>
<li>Benefits of upgrading from workstation to enterprise with the Ardia Platform</li>
</ul>
<blockquote>
Presenter: Seth Sheridan (Product Specialist III, Enterprise CDS, Americas, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Seth is an Enterprise CDS technical specialist who has supported Thermo Fisher’s Chromeleon software for over 3 years. Prior to this, he used Chromeleon and other CDS systems in labs at DuPont, DOW, and Eurofins. He holds a B.S. in Chemistry and Biochemistry and enjoys working closely with chromatographers to solve their software issues and improve their processes.
</blockquote>

Thermo Scientific: Learn the secrets of Chromeleon CDS: Expert advice and tips

Learn the secrets of Chromeleon CDS: Expert advice and tips

Fundamentals of Chromeleon CDS. How to create custom reports. New features in Chromeleon 7.3.2 CDS. Benefits of upgrading from workstation to enterprise.