Even in chromatography, appearances can be misleading. If you have ever performed a Liquid Chromatography (LC) or Gel Permeation Chromatography/Size Exclusion Chromatography (GPC/SEC) analysis and noticed unexpected signals appearing in your chromatogram, you may have encountered so-called chromatographic “ghosts.” These unanticipated peaks can complicate data interpretation and challenge even experienced analysts. Fortunately, these ghost peaks are not supernatural phenomena. In this Halloween-themed overview, we explore their origin, discuss strategies to minimize their occurrence, and describe practical approaches to identify them.<h4>What are ghost peaks?</h4>When a chromatogram displays additional peaks that cannot be directly attributed to analytes of interest, these signals are commonly referred to as ghost peaks or system peaks [1]. They arise when the equilibrium between the mobile phase and stationary phase is temporarily disturbed [1]. Such disturbances often occur during sample injection, when the system experiences a transient compositional or physical imbalance [1][2].Ghost peaks are particularly evident when using refractive index (RI) detection, where even injections of pure mobile phase can generate detectable signals (see Fig. 1). In GPC/SEC applications, these peaks frequently appear toward the end of the chromatogram, corresponding to the transition between size-based separation and interaction-driven effects [2]. Their appearance can closely resemble genuine analyte peaks and, in some cases, they may partially overlap with them, further complicating interpretation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_1_Chromatogram_of_a_20_l_blank_injection_mobile_phase_with_RI_detection_Graphic_by_KNAUER_72b81051f9.jpg" alt="KNAUER: Fig. 1 Chromatogram of a 20 µl blank injection (= mobile phase) with RI detection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_1_Chromatogram_of_a_20_l_blank_injection_mobile_phase_with_RI_detection_Graphic_by_KNAUER_72b81051f9.jpg 245w," sizes="100vw" width="1024" height="611"><h4>How to minimize ghost peaks?</h4>Ghost peaks may manifest as either positive or negative signals. While their retention time typically remains consistent, their intensity, shape, and area can vary depending on injection conditions, sample composition, and injection volume (see Fig. 2). Due to this variability, they are not suitable as reference markers, for example in GPC/SEC analyses.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_2_Overlay_chromatograms_measured_with_RID_and_20_l_injection_volume_c5b4b8f3f6.jpg" alt="KNAUER: Fig. 2 Overlay chromatograms measured with RID and 20 µl injection volume. Dotted black: citric acid; solid black: ethylene glycol. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_2_Overlay_chromatograms_measured_with_RID_and_20_l_injection_volume_c5b4b8f3f6.jpg 245w," sizes="100vw" width="1024" height="611">To reduce their impact, several practical measures can be implemented:<ul><li>Ensure that samples are dissolved in the same mobile phase used in the chromatographic system</li><li>Use smaller injection volumes to limit system disturbance</li><li>Allow sufficient equilibration time for the system before analysis</li></ul>Despite these precautions, ghost peaks cannot be completely eliminated. Therefore, recognizing and correctly assigning them is essential for reliable data interpretation. Failure to do so may lead to misinterpretation of chromatographic results.<h3>How to unmask ghost peaks?</h3>A systematic approach can help identify the origin of ghost peaks. One effective strategy involves preparing each component of the mobile phase individually, spiking them into the mobile phase, and injecting them separately.For example, in an aqueous SEC method using phosphate-buffered saline (PBS), individual injections of buffer components such as NaCl, KCl, KH₂PO₄, and Na₂HPO₄ can be compared with a blank injection (see Fig. 3). By overlaying these chromatograms, it becomes possible to assign specific ghost peaks to individual buffer constituents. This not only clarifies their origin but also provides an opportunity to optimize the method by adjusting buffer composition or concentration.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_3_Overlay_chromatograms_measured_with_RID_and_20_l_injection_volume_c5f57e44f6.jpg" alt="KNAUER: Fig. 3 Overlay chromatograms measured with RID and 20 µl injection volume. Black: blank (mobile phase = PBS); light green: sodium chloride; dark green: potassium chloride; light blue: potassium dihydrogen phosphate; dark blue: disodium hydrogen phosphate. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_3_Overlay_chromatograms_measured_with_RID_and_20_l_injection_volume_c5f57e44f6.jpg 245w," sizes="100vw" width="1024" height="611">Another practical approach involves comparing a blank chromatogram (mobile phase only) with that of the sample. Peaks that appear at identical retention times in both chromatograms can be confidently identified as ghost peaks.This concept is illustrated in Fig. 4 for an aqueous SEC system using PBS. Blank injections reveal two small peaks detected by the RI detector. In the case of citric acid, only two distinct peaks are observed instead of three, as one ghost peak coelutes with the analyte, forming a subtle shoulder (Fig. 4A). This demonstrates how ghost peaks can distort peak shapes and affect interpretation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_4_Left_4a_Overlay_of_chromatograms_recorded_with_the_RID_using_a_20_L_injection_volume_1732ef3a18.jpg" alt="KNAUER: Fig. 4 Left (4a): Overlay of chromatograms recorded with the RID using a 20 µL injection volume. Orange: citric acid; black: blank (mobile phase = PBS)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_4_Left_4a_Overlay_of_chromatograms_recorded_with_the_RID_using_a_20_L_injection_volume_1732ef3a18.jpg 245w," sizes="100vw" width="1024" height="611">In contrast, ethylene glycol produces three clearly resolved peaks (Fig. 4B), where ghost peaks do not interfere directly with the analyte signal. Nevertheless, their identification remains crucial to ensure accurate evaluation of the chromatographic data.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_4_Left_4b_Overlay_of_chromatograms_recorded_with_the_RID_using_a_20_L_injection_volume_149ed84d87.jpg" alt="KNAUER: Fig. 4b - Overlay of chromatograms recorded with the RID using a 20 µL injection volume. Orange: ethylene glycol; black: blank (mobile phase = PBS). Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_4_Left_4b_Overlay_of_chromatograms_recorded_with_the_RID_using_a_20_L_injection_volume_149ed84d87.jpg 245w," sizes="100vw" width="1024" height="611"><h4>Conclusion</h4>Ghost peaks are a common yet often misunderstood phenomenon in LC and GPC/SEC analyses. Although they may initially appear mysterious, they are the result of predictable physicochemical processes within the chromatographic system. By understanding their origin, applying appropriate experimental strategies, and using systematic identification approaches, analysts can effectively distinguish them from true analyte signals. Recognizing these “ghosts” ensures more accurate data interpretation and helps prevent misleading conclusions in chromatographic analysis.<blockquote><ul><li>If you’d like to learn more about detecting and minimizing system or ghost peaks in practice, take a look at our <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0046_knauer_understanding_system_peaks_in_gpc_sec_for_accurate_and_reliable_analysis_f134154935.pdf">Technical Note VTN0046</a> on identifying and reducing system peaks in GPC/SEC.</li><li>If you're planning to set up or optimize your GPC/SEC workflow, feel free to contact us at sales@knauer.net.&nbsp;</li><li>For further information on this topic, please contact our author: wesolowski@knauer.net</li></ul></blockquote>

GPC/SEC Made Easy – Unmask Ghost Peaks

News from LabRulezLCMS Library - Week 15, 2026

Traditional protein purification workflows in many laboratories still rely on ultracentrifugation and long incubation steps. These approaches often involve multi-step protocols, extended processing times, and the use of specialized equipment. As a result, workflows can become complex, time-consuming, and prone to variability.Magnetic bead-based protein purification offers a faster, simpler, and more efficient alternative by enabling selective capture and rapid magnetic separation.<h3>Limitations of Conventional Protein Cleanup Methods</h3>In standard laboratory workflows, protein cleanup typically involves:<ul><li>Multiple processing steps</li><li>Centrifugation and column equilibration</li><li>Dependence on specialized instrumentation</li><li>Long processing times</li></ul>These factors introduce several challenges:<ul><li>Increased complexity of workflows</li><li>Variability between runs, reducing reproducibility</li><li>Risk of sample loss during transfers, especially for small sample volumes or scarce targets</li><li>Limited throughput due to manual handling</li></ul><h3>Principle of Magnetic Bead-Based Purification</h3>Magnetic beads enable selective protein capture through surface functionalization.Each bead is coated with streptavidin, allowing the attachment of a biotinylated ligand. This ligand can be selected based on the target analyte and may include:<ul><li>Antibodies</li><li>Aptamers</li><li>Antigen fragments</li></ul>This functionalization step provides the beads with the specificity required to bind the target analyte.<h3>Materials and Key Components</h3><h4>Magnetic Beads</h4><ul><li>Streptavidin-coated surface</li><li>Functionalized with biotinylated ligands</li></ul><h3>Ligands</h3><ul><li>Selected for target specificity</li><li>Attached to beads prior to purification</li></ul><h3>Step-by-Step Procedure</h3><h4>1. Bead Activation</h4>Load the biotinylated ligand onto the streptavidin-coated magnetic beads to confer specificity.<h4>2. Washing</h4>Remove impurities after ligand loading to prepare the beads for interaction with the sample.<h4>3. Incubation with Sample</h4>Incubate the functionalized beads with the sample to allow binding between the ligand and the target analyte.<h4>4. Magnetic Separation</h4>Place the sample on a magnet:<ul><li>Magnetic beads (with bound analyte) are attracted to the vessel wall</li><li>The liquid phase can be separated easily</li></ul><h4>5. Washing</h4>Wash the beads while they remain immobilized by the magnetic field to remove:<ul><li>Weak interactions</li><li>Non-specific binding</li></ul><h4>6. Elution</h4>Add an elution solvent to release the purified analyte from the beads.<h3>Downstream Applications</h3>After elution, the purified sample is ready for further analysis, including:<ul><li>LC-MS</li><li>Western blot</li><li>ELISA</li><li>Activity assays</li></ul><h4>Advantages of Magnetic Bead-Based Workflows</h4>Magnetic bead-based purification offers several key benefits:<h4>Speed and Simplicity</h4><ul><li>No centrifugation required</li><li>No complex instrumentation needed</li></ul><h4>Improved Reproducibility</h4><ul><li>Consistent magnetic separation</li><li>Reduced variability between runs</li></ul><h4>Reduced Sample Loss</h4><ul><li>Fewer transfer steps</li><li>Suitable for small or scarce samples</li></ul><h4>Scalability and Throughput</h4><ul><li>Compatible with automation</li><li>Supports microplate formats:<ul><li>96-well plates</li><li>384-well plates</li></ul></li></ul><h3>Biozen™ Magnetic Beads Features</h3>Phenomenex Biozen™ magnetic beads incorporate several design features:<ul><li>Hydrophilic co-polymer surface<ul><li>Reduces non-specific hydrophobic interactions</li></ul></li><li>Biotinylated BSA spacer<ul><li>Acts as a linker to streptavidin</li><li>Helps orient capture ligands</li></ul></li><li>Monodispersed capture ligands<ul><li>Optimized binding capacity</li></ul></li></ul>These features contribute to:<ul><li>Reduced non-specific binding</li><li>Improved capture consistency</li><li>Cleaner eluates</li></ul><h3>When to Use Magnetic Beads</h3>Magnetic bead-based purification is particularly suitable when:<ul><li>Sample volumes are small</li><li>Fast turnaround is required</li><li>Minimal equipment is preferred</li><li>High-throughput workflows are needed</li></ul><h3>Conclusion</h3>Magnetic bead-based protein purification simplifies traditional workflows by eliminating centrifugation and reducing complexity. Through selective capture and efficient magnetic separation, it enables faster processing, improved reproducibility, and compatibility with high-throughput and automated systems.

Magnetic Bead-Based Protein Purification: A Faster and More Efficient Workflow

Article

Application

Academy

Video

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Shimadzu Corporation

This session will walk you through the importance of instrument performance in a typically a production or continuous use and regulated environments.
Understand the ways and means to maximise the usage time and minimize the downtime for Prominence-i. Let’s discuss more on consumables, replacement frequency and how to keep up our integrated LC!
Learning Objectives:
<ul>
<li>Maximizing Prominence-i productivity
</li>
<li>Avoiding Common Mistakes and Failures
</li>
<li>Best Instrument Operation and Maintenance Practises
</li>
</ul>
<blockquote>
Presenter: Chia Chun Chew (Regional Service Engineer, Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Mr Chia Chun Chew holds Bachelor Degree in Mechanical Engineering at Nanyang Technological University (NTU) Singapore, he joined Shimadzu Asia Pacific since 2015. He is specialize in HPLC empower him in support Regional technical issue and key trainer of Prominence-i to field engineers, he is ready to deepen his learning on LC/LCMS. Prior joining Shimadzu he was a field engineer supporting semiconductor manufacturing environment to upkeep equipments productivity, which has put him in good position to think from customer point of interest.
</blockquote>
<blockquote>
Presenter: Dhananjay Rajabhoj (Assistant Manager, Shimadzu Analytical (India) Pvt Ltd)
</blockquote>
<blockquote>
Mr. Dhananjay Rajabhoj holds Bachelor’s Degree of Engineering with specializations in Instrumentation and Control. He joined Shimadzu Analytical India Pvt Ltd since 2018 as Assistant Manager. He technically supports for LC/LCMS queries in pan India and responsible to conduct trainings on LC for field service engineers. Also, he involves critical trouble shooting cases as well as supporting sales cases for non-routing configuration. Previously he has served Healthcare and Pharmaceutical industry for last more than 15 years.
</blockquote>

Shimadzu: Prominence-i | User Maintenance Demonstration and Discussion

Prominence-i | User Maintenance Demonstration and Discussion

The importance of instrument performance in a typically a production or continuous use and regulated environments.

This series is perfect for anyone currently using Preparative LC, who&#39;d like to understand more, as well as those new to the technique or eager to learn more.
Learning Objectives:
<ul>
<li>Differences between Semi-micro and Analytical Scale Prep
</li>
<li>Things to consider in semi-micro and analytical scale purification
</li>
<li>Instrumentation and software advantages
</li>
</ul>
<blockquote>
Presenter: Earl Mckoy (Senior LC/LCMS Technical Specialist, Shimadzu UK)
</blockquote>

Shimadzu: Semi-micro to Analytical Scale LC Purification

Semi-micro to Analytical Scale LC Purification

Differences between Semi-micro and Analytical Scale Prep.

This series is perfect for anyone currently using Preparative LC, who&#39;d like to understand more, as well as those new to the technique or eager to learn more.
Learning Objectives:
<ul>
<li>Principles, common issues and tips and tricks of Semi-Preparative Chromatography
</li>
<li>UFPLC - Ultra Fast Preparative and Purification LC
</li>
<li>Semi-preparative SFC Scale Purification
</li>
</ul>
<blockquote>
Presenter: Earl Mckoy (Senior LC/LCMS Technical Specialist, Shimadzu UK)
</blockquote>

Shimadzu: Semi-Preparative Scale LC Purification

Semi-Preparative Scale LC Purification

Principles, common issues and tips and tricks of Semi-Preparative Chromatography.

The objective of this webinar is to guide LC users on LC column care &amp; troubleshooting.
Audience will learn about ways to maintain LC column performance and prolong column lifetime. Guidance to troubleshoot issues caused by LC column will be share in this webinar.
Learning Objectives:
<ul>
<li>Learning on maintaining LC column performance and prolong column lifetime
</li>
<li>Troubleshooting issues caused by LC column
</li>
</ul>
<blockquote>
Presenter: Yap Siew Qi (Ph.D.) (Consumables Technical Specialist, Shimadzu (Asia Pacific) Pte. Ltd.)
</blockquote>
<blockquote>
Yap Siew Qi is Consumables Technical Specialist at Shimadzu Asia Pacific. She joined the company after obtaining her PhD from National University of Singapore. At Shimadzu, she is responsible for the technical support of consumables in chromatography and spectroscopy. The products include GC/LC columns, UV cuvettes, vials and accessories. Her responsibilities include technical support of sales/engineers, customer support and producing applications.
</blockquote>

Shimadzu: LC Column Care & Troubleshooting

LC Column Care & Troubleshooting

Learn about ways to maintain LC column performance and prolong column lifetime.

As one of the most prominent innovators in LC technology, Shimadzu has set the New Benchmark in the LC innovation by developing the very first AI-enabled LC system, which allows Chemists to spend less time operating, and handling the instrument, as the instrument with its in-built Analytical Intelligence algorithm enables the system of performing automation workflow.
The ultimate objective is to achieve minimized system downtime, optimize efficiency &amp; maximize productivity, through the New Benchmarks in Intelligence, Efficiency and Design.
Learning Objectives:
<ul>
<li>Explore the New Benchmark of Intelligence of New Nexera Series
</li>
<li>Understand the New Benchmark of Efficiency in delivering higher performance and throughput
</li>
<li>Be intrigued by the New Benchmark of Design which promises full system expansion and flexibility
</li>
</ul>
<blockquote>
Presenter: David Chan (Assistant Product Manager, Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Graduated from National University of Singapore, Chemistry major. David has acquired more than 10 years of experience in Medicinal Chemistry research for New Drug Discovery. Also, he has also accumulated 2 years of working experience as an Analytical Chemist. He has been with Shimadzu Asia Pacific for 7 years as Product Specialist for LC, providing technical advice to client as well as sales.
</blockquote>

Shimadzu: New Nexera Series, the First AI Enabled and IoT Driven LC

New Nexera Series, the First AI Enabled and IoT Driven LC

Explore the New Benchmark of Intelligence of New Nexera Series.

This series is perfect for anyone currently using Preparative LC, who&#39;d like to understand more, as well as those new to the technique or eager to learn more.
<ul>
<li>What is LC purification and how do you get the most out of the technique.
</li>
<li>How much sample can you expect to purify?
</li>
<li>Up-scaling basics
</li>
<li>Recycling and concentration
</li>
</ul>
<blockquote>
Presenter: Richard Hodgson (Pharmaceutical Market Specialist, Shimadzu UK Limited)
</blockquote>

Shimadzu: Fundamentals of LC Purification

Fundamentals of LC Purification

What is LC purification and how do you get the most out of the technique.

This webinar gives you an introduction on fully automated pre-column derivatization of the amino acids and microwave digestion method which were adopted to further speed up and increase the analysis efficiency.
A Shimadzu Nexera LC-40X3 with photodiode array (PDA) and fluorescence (RF) detectors were employed for the analysis. The Nexera LC-40X3 equipped with automated pre-treatment function allows several derivatization reagents to be aspirated sequentially from multiple vials and mixing within the needle and the sample loop. Microwave digestion procedure was employed to carry out both acid and alkaline hydrolysis in less than 30 minutes, which is much faster in comparison to the conventional hydrolysis up to 24 hours. Fluorescent (RF) detection was used for high sensitivity analysis (nM), while UV detection was compared, which shows its applicability for µM level quantitation of amino acids.
Learning Objectives:
<ul>
<li>Background of amino acid analysis in feed 
</li>
<li>Comparison between manual vs automated pre-column derivatization
</li>
<li>Sample preparation using conventional hydrolysis vs microwave digestion
</li>
</ul>
<blockquote>
Presenter: Liw Wan Tung (Senior Application Chemist, Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Wan Tung, a senior application chemist, has been with Shimadzu for 8 years. She has 11 years of working experience in analytical science, specializing in HPLC. Wan Tung’s recent research work at Shimadzu has mainly focused on fully automated pre-column derivatization of amino acids in a variety of food and biopharmaceutical sample matrices. Prior to joining Shimadzu, Wan Tung started her professional career as a QAQC chemist in Pharmaceutical multinational company. Wan Tung earned her bachelor’s honours degree in chemistry in 2009.
</blockquote>

Shimadzu: Online Pre-column Derivatization and UHPLC Analysis of Total Amino Acids in Animal Feed

Online Pre-column Derivatization and UHPLC Analysis of Total Amino Acids in Animal Feed

Introduction on fully automated pre-column derivatization of the amino acids and microwave digestion method.

Online Pre-column Derivatization and UHPLC Analysis

This webcast will consist of two parts. Part 1 will start off with a basic introduction to UV/Vis/NIR integrating spheres.
We will then consider samples, such as plastic films and colored Spectralon®, that illustrate how sphere diameter can impact spectral accuracy in both reflectance and scatter transmission measurements. This section will conclude with a look at how sample surface texture and sample placement against the sphere’s port, can result in unusual artifacts in the spectra.
Part 2 will discuss two novel integrating sphere applications within the rapidly growing technology field of nanomaterials.
Carbon nanotubes constitute a large arena of products where UV/Vis/NIR spectroscopy, employing an integrating sphere, is functional in both research and quality control. The webcast will conclude with a detailed look at the UV/Vis/NIR spectroscopy of butterfly wings. The colors on most butterfly wings do not arise from either dyes or pigments, but from nanostructures that physically diffract, rather than absorb light. As such, they represent an interesting platform to investigate the potential for spectroscopic materials characterization of nanostructures attached to surfaces.
<blockquote>
Presenter: Jeffrey L. Taylor, Ph.D. (Molecular Spectroscopy Guru, Industry Expert With Over 35 Years of Experience)
</blockquote>
<blockquote>
Dr. Jeffrey L. Taylor received his Bachelor of Science degree from Georgia College and State University with dual majors in Physiology and Organic Chemistry. He obtained a Master of Science degree from the University of Georgia in Toxicology and later a Doctor of Philosophy degree from the same school in Biochemistry and Spectroscopy.
</blockquote>
<blockquote>
He has spent his career of over 35 years with major spectroscopy instruments manufacturers, mainly working as Field Product Specialist and Senior Staff Scientist. Areas of expertise include technical support of sales, customer support/training, applications design and development, software specification/evaluation, and instrumental design and evaluation. He is the author of numerous application notes, conference presentations and webcasts, and holder of several patents.
</blockquote>
<blockquote>
Dr. Taylor, although retired, maintains his desire to contribute to the field. He is currently a consultant for Shimadzu Scientific Instruments to assist with sales support, application support and development of web-based educational resources.
</blockquote>
<blockquote>
Presenter: Sudhir Dahal, Ph.D. (Product Manager - Molecular Spectroscopy, Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Sudhir Dahal is Product Manager of Molecular Spectroscopy group at Shimadzu Scientific Instruments, USA. He joined the company after obtaining his PhD from University of Maryland Baltimore County (UMBC), where he researched and collaborated on developing novel spectroscopy-based technique for brain tumor cell detection. At Shimadzu Scientific Instruments, he and his group are responsible for overall sales support of molecular spectroscopy products in North America, Central America and parts of South America. The products include UV-Vis-NIR, FTIR, Fluorescence spectrophotometers and accessories.
</blockquote>
<blockquote>
His everyday responsibilities include, but are not limited to, teaching classes to sales/technical support engineers and customers, producing scientific literatures, researching new products and applications, representing Shimadzu at conferences and promoting the products through various marketing campaigns.
</blockquote>

Shimadzu: Plastic Films, Fabrics, Nano Particles, and Butterfly Wings: Novel, “Outside the Box”, Uses For UV/Vis/NIR Integrating Spheres

Plastic Films, Fabrics, Nano Particles, and Butterfly Wings: Novel, “Outside the Box”, Uses For UV/Vis/NIR Integrating Spheres

Basic introduction to UV/Vis/NIR integrating spheres. Two novel integrating sphere applications within the rapidly growing technology field of nanomaterials.

Plastic Films, Fabrics, Nano Particles, and Butterfly Wings

Most people think solutions in a cuvette when referring to spectrophotometers; however, in the world of materials characterization, rarely are solutions the primary sample.
Here the samples can be anything from solid, creams, gels, particle suspensions, films, and in some cases actual human beings (sunscreen characterization). These samples all have one thing in common, they are impossible to dilute. The materials characterization high performance spectrophotometer must have the ability to measure high absorbance values (8 to 10 absorbance units). Instrumental parameters that are crucial to measuring materials characterization samples are stray light, noise, resolution, and scan speed. In this presentation we will address how these inter-related parameters influence measurement success.
Most measurements for the above-mentioned samples are not of the simple transmission type. Reflection, both specular and diffuse, and scatter transmission measurements abound in the world of materials characterization. It is vital that the proper accessory be employed to match the measurement desired. This presentation will address the function of integrating spheres and how they can be used to measure both diffuse reflection and transmission. Also discussed will be the difference between a relative and absolute reflection measurement. Finally, an overview will be given of important accessories related to the materials characterization marketplace. Register now!
<blockquote>
Presenter: Jeffrey L. Taylor, Ph.D. (Molecular Spectroscopy Guru, Industry Expert With Over 35 Years of Experience)
</blockquote>
<blockquote>
Dr. Jeffrey L. Taylor received his Bachelor of Science degree from Georgia College and State University with dual majors in Physiology and Organic Chemistry. He obtained a Master of Science degree from the University of Georgia in Toxicology and later a Doctor of Philosophy degree from the same school in Biochemistry and Spectroscopy.
</blockquote>
<blockquote>
He has spent his career of over 35 years with major spectroscopy instruments manufacturers, mainly working as Field Product Specialist and Senior Staff Scientist. Areas of expertise include technical support of sales, customer support/training, applications design and development, software specification/evaluation, and instrumental design and evaluation. He is the author of numerous application notes, conference presentations and webcasts, and holder of several patents.
</blockquote>
<blockquote>
Dr. Taylor, although retired, maintains his desire to contribute to the field. He is currently a consultant for Shimadzu Scientific Instruments to assist with sales support, application support and development of web-based educational resources.
</blockquote>
<blockquote>
Presenter: Sudhir Dahal, Ph.D. (Product Manager - Molecular Spectroscopy, Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Sudhir Dahal is Product Manager of Molecular Spectroscopy group at Shimadzu Scientific Instruments, USA. He joined the company after obtaining his PhD from University of Maryland Baltimore County (UMBC), where he researched and collaborated on developing novel spectroscopy-based technique for brain tumor cell detection. At Shimadzu Scientific Instruments, he and his group are responsible for overall sales support of molecular spectroscopy products in North America, Central America and parts of South America. The products include UV-Vis-NIR, FTIR, Fluorescence spectrophotometers and accessories.
</blockquote>
<blockquote>
His everyday responsibilities include, but are not limited to, teaching classes to sales/technical support engineers and customers, producing scientific literatures, researching new products and applications, representing Shimadzu at conferences and promoting the products through various marketing campaigns.
</blockquote>

Shimadzu: UV/Vis/NIR Spectroscopy in the Arena of Materials Characterization Research and Quality Control: How Instrumental Performance and Accessories Define Success

UV/Vis/NIR Spectroscopy in the Arena of Materials Characterization Research and Quality Control: How Instrumental Performance and Accessories Define Success

The function of integrating spheres and how they can be used to measure both diffuse reflection and transmission.

UV/Vis/NIR Spectroscopy in the Arena of Materials

As the world grapples with COVID-19 pandemic and plans for the aftermath, digitalization of laboratory workspace has become more urgent than ever before.
In the future, most scientists will be telecommuting - away from the laboratory – for data analysis and reporting. Time spent in the laboratory itself will be solely for the purpose of analysing samples and pushing data into a secure database to be retrieved remotely. In this 45-min webinar, we will be discussing Shimadzu’s Informatics solutions – how different workflow solutions will facilitate the digitalization of your laboratory workspace by connectng your laboratory instruments, saving the data as well as allowing remote access and control for the users.
Learning Objectives
<ul>
<li>Understand how your team can start preparing your laboratory to the New Normal with Informatics Solutions
</li>
<li>Explore technologies that allows remote access to your laboratory from anywhere
</li>
<li>Implement effective data management and increasing productivity &amp; compliance in laboratories
</li>
</ul>
<blockquote>
Presenter: Sateesh Tummala (Assistant Manager, IT &amp; Informatics, Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Sateesh is associated with Shimadzu since beginning of his career. He started with technical support in India and expanded his scope for supporting ASEAN and South Asian countries. He has wide experience in Pharma domain and well versed with regulatory compliance guidelines. Sateesh is currently leading the informatics team in Shimadzu Asia Pacific, Singapore leading promotion and technical support of Shimadzu Informatics solutions and Caliber LIMS products in the region.
</blockquote>

Shimadzu: Digital Transformation of Laboratory Workspace – Enhancing Workflow with Analytical Intelligence

Digital Transformation of Laboratory Workspace – Enhancing Workflow with Analytical Intelligence

How different workflow solutions will facilitate the digitalization of your laboratory workspace by connectng your laboratory instruments.