Even in chromatography, appearances can be misleading. If you have ever performed a Liquid Chromatography (LC) or Gel Permeation Chromatography/Size Exclusion Chromatography (GPC/SEC) analysis and noticed unexpected signals appearing in your chromatogram, you may have encountered so-called chromatographic “ghosts.” These unanticipated peaks can complicate data interpretation and challenge even experienced analysts. Fortunately, these ghost peaks are not supernatural phenomena. In this Halloween-themed overview, we explore their origin, discuss strategies to minimize their occurrence, and describe practical approaches to identify them.<h4>What are ghost peaks?</h4>When a chromatogram displays additional peaks that cannot be directly attributed to analytes of interest, these signals are commonly referred to as ghost peaks or system peaks [1]. They arise when the equilibrium between the mobile phase and stationary phase is temporarily disturbed [1]. Such disturbances often occur during sample injection, when the system experiences a transient compositional or physical imbalance [1][2].Ghost peaks are particularly evident when using refractive index (RI) detection, where even injections of pure mobile phase can generate detectable signals (see Fig. 1). In GPC/SEC applications, these peaks frequently appear toward the end of the chromatogram, corresponding to the transition between size-based separation and interaction-driven effects [2]. Their appearance can closely resemble genuine analyte peaks and, in some cases, they may partially overlap with them, further complicating interpretation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_1_Chromatogram_of_a_20_l_blank_injection_mobile_phase_with_RI_detection_Graphic_by_KNAUER_72b81051f9.jpg" alt="KNAUER: Fig. 1 Chromatogram of a 20 µl blank injection (= mobile phase) with RI detection. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_1_Chromatogram_of_a_20_l_blank_injection_mobile_phase_with_RI_detection_Graphic_by_KNAUER_72b81051f9.jpg 245w," sizes="100vw" width="1024" height="611"><h4>How to minimize ghost peaks?</h4>Ghost peaks may manifest as either positive or negative signals. While their retention time typically remains consistent, their intensity, shape, and area can vary depending on injection conditions, sample composition, and injection volume (see Fig. 2). Due to this variability, they are not suitable as reference markers, for example in GPC/SEC analyses.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_2_Overlay_chromatograms_measured_with_RID_and_20_l_injection_volume_c5b4b8f3f6.jpg" alt="KNAUER: Fig. 2 Overlay chromatograms measured with RID and 20 µl injection volume. Dotted black: citric acid; solid black: ethylene glycol. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_2_Overlay_chromatograms_measured_with_RID_and_20_l_injection_volume_c5b4b8f3f6.jpg 245w," sizes="100vw" width="1024" height="611">To reduce their impact, several practical measures can be implemented:<ul><li>Ensure that samples are dissolved in the same mobile phase used in the chromatographic system</li><li>Use smaller injection volumes to limit system disturbance</li><li>Allow sufficient equilibration time for the system before analysis</li></ul>Despite these precautions, ghost peaks cannot be completely eliminated. Therefore, recognizing and correctly assigning them is essential for reliable data interpretation. Failure to do so may lead to misinterpretation of chromatographic results.<h3>How to unmask ghost peaks?</h3>A systematic approach can help identify the origin of ghost peaks. One effective strategy involves preparing each component of the mobile phase individually, spiking them into the mobile phase, and injecting them separately.For example, in an aqueous SEC method using phosphate-buffered saline (PBS), individual injections of buffer components such as NaCl, KCl, KH₂PO₄, and Na₂HPO₄ can be compared with a blank injection (see Fig. 3). By overlaying these chromatograms, it becomes possible to assign specific ghost peaks to individual buffer constituents. This not only clarifies their origin but also provides an opportunity to optimize the method by adjusting buffer composition or concentration.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_3_Overlay_chromatograms_measured_with_RID_and_20_l_injection_volume_c5f57e44f6.jpg" alt="KNAUER: Fig. 3 Overlay chromatograms measured with RID and 20 µl injection volume. Black: blank (mobile phase = PBS); light green: sodium chloride; dark green: potassium chloride; light blue: potassium dihydrogen phosphate; dark blue: disodium hydrogen phosphate. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_3_Overlay_chromatograms_measured_with_RID_and_20_l_injection_volume_c5f57e44f6.jpg 245w," sizes="100vw" width="1024" height="611">Another practical approach involves comparing a blank chromatogram (mobile phase only) with that of the sample. Peaks that appear at identical retention times in both chromatograms can be confidently identified as ghost peaks.This concept is illustrated in Fig. 4 for an aqueous SEC system using PBS. Blank injections reveal two small peaks detected by the RI detector. In the case of citric acid, only two distinct peaks are observed instead of three, as one ghost peak coelutes with the analyte, forming a subtle shoulder (Fig. 4A). This demonstrates how ghost peaks can distort peak shapes and affect interpretation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_4_Left_4a_Overlay_of_chromatograms_recorded_with_the_RID_using_a_20_L_injection_volume_1732ef3a18.jpg" alt="KNAUER: Fig. 4 Left (4a): Overlay of chromatograms recorded with the RID using a 20 µL injection volume. Orange: citric acid; black: blank (mobile phase = PBS)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_4_Left_4a_Overlay_of_chromatograms_recorded_with_the_RID_using_a_20_L_injection_volume_1732ef3a18.jpg 245w," sizes="100vw" width="1024" height="611">In contrast, ethylene glycol produces three clearly resolved peaks (Fig. 4B), where ghost peaks do not interfere directly with the analyte signal. Nevertheless, their identification remains crucial to ensure accurate evaluation of the chromatographic data.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_4_Left_4b_Overlay_of_chromatograms_recorded_with_the_RID_using_a_20_L_injection_volume_149ed84d87.jpg" alt="KNAUER: Fig. 4b - Overlay of chromatograms recorded with the RID using a 20 µL injection volume. Orange: ethylene glycol; black: blank (mobile phase = PBS). Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_4_Left_4b_Overlay_of_chromatograms_recorded_with_the_RID_using_a_20_L_injection_volume_149ed84d87.jpg 245w," sizes="100vw" width="1024" height="611"><h4>Conclusion</h4>Ghost peaks are a common yet often misunderstood phenomenon in LC and GPC/SEC analyses. Although they may initially appear mysterious, they are the result of predictable physicochemical processes within the chromatographic system. By understanding their origin, applying appropriate experimental strategies, and using systematic identification approaches, analysts can effectively distinguish them from true analyte signals. Recognizing these “ghosts” ensures more accurate data interpretation and helps prevent misleading conclusions in chromatographic analysis.<blockquote><ul><li>If you’d like to learn more about detecting and minimizing system or ghost peaks in practice, take a look at our <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0046_knauer_understanding_system_peaks_in_gpc_sec_for_accurate_and_reliable_analysis_f134154935.pdf">Technical Note VTN0046</a> on identifying and reducing system peaks in GPC/SEC.</li><li>If you're planning to set up or optimize your GPC/SEC workflow, feel free to contact us at sales@knauer.net.&nbsp;</li><li>For further information on this topic, please contact our author: wesolowski@knauer.net</li></ul></blockquote>

GPC/SEC Made Easy – Unmask Ghost Peaks

News from LabRulezLCMS Library - Week 15, 2026

Traditional protein purification workflows in many laboratories still rely on ultracentrifugation and long incubation steps. These approaches often involve multi-step protocols, extended processing times, and the use of specialized equipment. As a result, workflows can become complex, time-consuming, and prone to variability.Magnetic bead-based protein purification offers a faster, simpler, and more efficient alternative by enabling selective capture and rapid magnetic separation.<h3>Limitations of Conventional Protein Cleanup Methods</h3>In standard laboratory workflows, protein cleanup typically involves:<ul><li>Multiple processing steps</li><li>Centrifugation and column equilibration</li><li>Dependence on specialized instrumentation</li><li>Long processing times</li></ul>These factors introduce several challenges:<ul><li>Increased complexity of workflows</li><li>Variability between runs, reducing reproducibility</li><li>Risk of sample loss during transfers, especially for small sample volumes or scarce targets</li><li>Limited throughput due to manual handling</li></ul><h3>Principle of Magnetic Bead-Based Purification</h3>Magnetic beads enable selective protein capture through surface functionalization.Each bead is coated with streptavidin, allowing the attachment of a biotinylated ligand. This ligand can be selected based on the target analyte and may include:<ul><li>Antibodies</li><li>Aptamers</li><li>Antigen fragments</li></ul>This functionalization step provides the beads with the specificity required to bind the target analyte.<h3>Materials and Key Components</h3><h4>Magnetic Beads</h4><ul><li>Streptavidin-coated surface</li><li>Functionalized with biotinylated ligands</li></ul><h3>Ligands</h3><ul><li>Selected for target specificity</li><li>Attached to beads prior to purification</li></ul><h3>Step-by-Step Procedure</h3><h4>1. Bead Activation</h4>Load the biotinylated ligand onto the streptavidin-coated magnetic beads to confer specificity.<h4>2. Washing</h4>Remove impurities after ligand loading to prepare the beads for interaction with the sample.<h4>3. Incubation with Sample</h4>Incubate the functionalized beads with the sample to allow binding between the ligand and the target analyte.<h4>4. Magnetic Separation</h4>Place the sample on a magnet:<ul><li>Magnetic beads (with bound analyte) are attracted to the vessel wall</li><li>The liquid phase can be separated easily</li></ul><h4>5. Washing</h4>Wash the beads while they remain immobilized by the magnetic field to remove:<ul><li>Weak interactions</li><li>Non-specific binding</li></ul><h4>6. Elution</h4>Add an elution solvent to release the purified analyte from the beads.<h3>Downstream Applications</h3>After elution, the purified sample is ready for further analysis, including:<ul><li>LC-MS</li><li>Western blot</li><li>ELISA</li><li>Activity assays</li></ul><h4>Advantages of Magnetic Bead-Based Workflows</h4>Magnetic bead-based purification offers several key benefits:<h4>Speed and Simplicity</h4><ul><li>No centrifugation required</li><li>No complex instrumentation needed</li></ul><h4>Improved Reproducibility</h4><ul><li>Consistent magnetic separation</li><li>Reduced variability between runs</li></ul><h4>Reduced Sample Loss</h4><ul><li>Fewer transfer steps</li><li>Suitable for small or scarce samples</li></ul><h4>Scalability and Throughput</h4><ul><li>Compatible with automation</li><li>Supports microplate formats:<ul><li>96-well plates</li><li>384-well plates</li></ul></li></ul><h3>Biozen™ Magnetic Beads Features</h3>Phenomenex Biozen™ magnetic beads incorporate several design features:<ul><li>Hydrophilic co-polymer surface<ul><li>Reduces non-specific hydrophobic interactions</li></ul></li><li>Biotinylated BSA spacer<ul><li>Acts as a linker to streptavidin</li><li>Helps orient capture ligands</li></ul></li><li>Monodispersed capture ligands<ul><li>Optimized binding capacity</li></ul></li></ul>These features contribute to:<ul><li>Reduced non-specific binding</li><li>Improved capture consistency</li><li>Cleaner eluates</li></ul><h3>When to Use Magnetic Beads</h3>Magnetic bead-based purification is particularly suitable when:<ul><li>Sample volumes are small</li><li>Fast turnaround is required</li><li>Minimal equipment is preferred</li><li>High-throughput workflows are needed</li></ul><h3>Conclusion</h3>Magnetic bead-based protein purification simplifies traditional workflows by eliminating centrifugation and reducing complexity. Through selective capture and efficient magnetic separation, it enables faster processing, improved reproducibility, and compatibility with high-throughput and automated systems.

Magnetic Bead-Based Protein Purification: A Faster and More Efficient Workflow

Article

Application

Academy

Video

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Separation Science

Agilent Technologies

There is currently a great deal of regulatory focus on data integrity, especially in the QC Laboratory.
This is causing pharmaceutical (and other) companies to re-examine their practices and processes and their use of analytical instruments and data management systems in order to not only assure data integrity, but to do so in a way that is capable of demonstrating compliance with international regulatory requirements.
This webinar will present on the topic of laboratory data integrity, examining this from the perspective of Laboratory Management, Supervisors and Technicians.
Topics covered will include:
<ul>
<li>What are the risks associated with a lack of data integrity and a background and overview of current and future regulatory trends
</li>
<li>Current regulatory guidance
</li>
<li>How risks to data integrity arise and what to do to address the cultural and technical risks
</li>
<li>How modern laboratory instruments and data management systems can assure technical compliance with data integrity requirements
</li>
<li>Intended Audience: Laboratory Data Owners (Managers) and Data Stewards (Laboratory Supervisors and Technicians).
</li>
</ul>
By viewing this webinar you will learn:
<ul>
<li>Why data integrity is important in assuring product quality and patient safety
</li>
<li>Why there is a significant focus on data integrity amongst international regulators
</li>
<li>Key steps to take to assure data integrity in the laboratory
</li>
</ul>
<blockquote>
Presenter: David Stokes (Principal Consultant and Director, Convalido Consulting Ltd, Newcastle-Upon-Tyne, UK)
</blockquote>

Shimadzu: Assuring Laboratory Data Integrity in a Time of Enhanced Regulatory Oversight

Assuring Laboratory Data Integrity in a Time of Enhanced Regulatory Oversight

Laboratory data integrity, examining this from the perspective of Laboratory Management, Supervisors and Technicians.

Assuring Laboratory Data Integrity

By automating the links between CDS and LIMS, a lab cam increase productivity and reduce errors.
Agilent has multiple ways to help labs do this for Agilent CDS systems as well as non-CDS data sources. This talk will give an overview of tools that help labs achieve bi-directional direct integration of sample information and results from Agilent CDS plus other sources and LIMS.
<blockquote>
Presenter: Dr. Ute Bober (Informatics Division, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Ute Bober is a Product Owner for the Software and Informatics Division at Agilent Technologies, where she focuses on delivering, maintaining and marketing software for effective laboratory workflow management. Ute has more than 20 years of professional experience in informatics. She started her career as an analytical research chemist and soon took the opportunity to develop towards software and informatics by working in field support, product management and product marketing at Hewlett Packard and Agilent Technologies. Since 2013 she is bringing here knowledge into the field of technical marketing where she currently owns the Sample Scheduler for OpenLab.
</blockquote>

Agilent Technologies: Increase Throughput and Reduce Errors by Automating LIMS Connectivity

Increase Throughput and Reduce Errors by Automating LIMS Connectivity

Overview of tools that help labs achieve bi-directional direct integration of sample information and results from Agilent CDS plus other sources and LIMS.

Increase Throughput and Reduce Errors by Automating LIMS

Your quest for a lab system to increase the automation and achieve a higher throughput is now compromised by the requirements for remote working and social distancing.
At the same time, a lab cannot operate without people actually being physically in the lab. So how can we minimize staff presence on premises while maintaining operations? In this session, we will discuss how to balance these two objectives and how to optimize working remotely including discussions around data security and operative aspects. Key Learning Objectives. Learn what it takes to successfully implement an end-to-end solution to manage all content, context, and results in the lab. Discuss aspects to consider to evaluate, implement and operate a remote Lab Workflow solution. Tips for how to make physical and digital processes come together.
<blockquote>
Presenter: Nicolas Louvet (Software Product Manager, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Nicolas Louvet joined Agilent Technologies in 2005 as an ELN Services Consultant in the Software &amp; Informatics Division, became the Product Manager for OpenLab ELN, and currently serves as the Software Product Manager for SLIMS. He has more than 15 years of experience working directly with clients needing to digitalize their laboratory processes. His area of expertise is focused on workflow and content management in the laboratory.
</blockquote>

Agilent Technologies: Laboratory Workflow Solutions: What to expect when you’re away from the lab?

Laboratory Workflow Solutions: What to expect when you’re away from the lab?

How to balance these two objectives and how to optimize working remotely including discussions around data security and operative aspects.

What to expect when you’re away from the lab?

The modern laboratory has data collected and reported from a variety of different instruments and locations.
Agilent’s Enterprise Content Manager can store that data and make metadata available for searching through a variety of data specific filters or customized PDF templates. The Metadata ReUse program can then help query and combine that data into an XML/Excel format for further reporting.
Key Learning Objectives
<ul>
<li>Introduction to the ECM-XT Web Client Interface
</li>
<li>MetaData Reuse tool features overview
</li>
<li>XML/Excel Export function of the the MetaData Reuse Tool
</li>
</ul>
<blockquote>
Presenter: Richard Mutkoski (Laboratory Informatics Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Richard Mutkoski began with Agilent (HP) in 1995. He has been an Application Engineer in Agilent’s Laboratory Informatics for the past 18 years. Prior to his career at Agilent Rich spent 12 years in a variety of small pharmaceutical firms in both R&amp;D and QA/QC capacities affording him first-hand knowledge of those workflows, bottlenecks and challenges.
</blockquote>

Agilent Technologies: The Power of Using Existing Meta-Data for Lab Wide Reporting

The Power of Using Existing Meta-Data for Lab Wide Reporting

Introduction to the ECM-XT Web Client Interface.

The promises of the digital lab are enticing but getting there sometimes seems daunting.
This presentation will discuss how to digitize lab operations from start to finish, which is especially relevant in a world where working remotely is becoming increasingly common. In the course of this journey, how can you realize your aspirations and create sustainable outcomes, all while dealing with varied lab environments? The good news is that you can start today. This talk will show how you can prepare the ground for a digital lab, how to improve efficiency of your lab operations, and what it takes to implement an end-to-end solution to manage all content, context, and results in the lab.
<blockquote>
Presenter: Nicolas Louvet (Software Product Manager, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Nicolas Louvet joined Agilent Technologies in 2005 as an ELN Services Consultant in the Software &amp; Informatics Division, became the Product Manager for OpenLab ELN, and currently serves as the Software Product Manager for SLIMS. He has more than 15 years of experience working directly with clients needing to digitalize their laboratory processes. His area of expertise is focused on workflow and content management in the laboratory.
</blockquote>

Agilent Technologies: How to Achieve a Fully Digital, End-to-End Analytical Workflow with CDS, ELN and LIMS

How to Achieve a Fully Digital, End-to-End Analytical Workflow with CDS, ELN and LIMS

How to digitize lab operations from start to finish, which is especially relevant in a world where working remotely is becoming increasingly common.

How to Achieve a Fully Digital, End-to-End Workflow

In this seminar we will build a qualitative method for peak purity.
We will look at an LC/MS walk up method work flow within the OpenLab CDS environment. We will look at LC/MS purity functions, the summing of SIM ions and spectral tips and tricks. 
<blockquote>
Presenter: Sue D&#39;Antonio (Applications Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Sue D’Antonio joined Agilent (HP at that time) more than 30 years ago. For the last 15 years Sue has been an Applications Scientist in the Liquid Phase Separations Group. Sue’s application support extends to HPLC, UHPLC, CE and LCMS across a wide range of markets and customers.
</blockquote>

Agilent Technologies: Tips and Tricks for LC/MS Purity workflow in OpenLab CDS softwar

Tips and Tricks for LC/MS Purity workflow in OpenLab CDS softwar

LC/MS walk up method work flow within the OpenLab CDS environment.

Tips and Tricks for LC/MS Purity workflow in OpenLab CDS

This webinar will give an overview of Agilent’s Walkup add-on software which can increase the efficiency of LC and LC/MS systems with multiple users.
The Walkup software allows for an administrator to oversee all aspects of the chromatography system and defines specified user access. A general overview of the Walkup software platform will be given with examples utilized to show the convenience for a multiple user chromatography system.

Agilent Technologies: Inc the Spd, Effic, Robustness, and Ease of Operation of Your Multi-use LC and LC/MS Sys by Incorporating Agilent

Inc the Spd, Effic, Robustness, and Ease of Operation of Your Multi-use LC and LC/MS Sys by Incorporating Agilent

Overview of Agilent’s Walkup add-on software which can increase the efficiency of LC and LC/MS systems with multiple users.

Inc the Spd, Effic, Robustness, and Ease of Operation

HPLC Autosampler injector programming has been a feature of Agilent autosamplers since the early days of autosampler innovations.
The injector program contains a series of simple commands that maybe performed during an injection cycle which enables a sample pretreatment. During this session we will discuss how these commands can be employed to make dilutions to reduce sample preparation time. The versatility of the program allows multi-level dilutions with minimum carryover.
<blockquote>
Presenter: Greg Hunlen (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Gregory Hunlen has served as an Application Scientist with Agilent Technologies since 2012. He applies his expertise to the Agilent Infinity II series of LC instruments, providing knowledge of LC solutions such as two dimensional LC and LCMSD workflows. He also delivers product demonstrations and lunch &amp; learns. As a key value, Greg likes to talk about maximizing the use of all features our technologies provide. Often, instruments are under-utilized perhaps as a lack of understanding of all that they can offer. When we explore these features, we discover that we can truly optimize the efficiencies of our instruments. Prior to joining Agilent, Greg spent 20 + years in the pharmaceutical research industry on the liquid phase chromatography side of drug development.
</blockquote>

Agilent Technologies: Strategies for HPLC Analysis using Autosampler Injector Programming

Strategies for HPLC Analysis using Autosampler Injector Programming

How these commands can be employed to make dilutions to reduce sample preparation time.

Strategies for HPLC Analysis using Autosampler Injector

Find out about the next generation of software from Agilent.
OpenLab CDS can streamline your lab operations with a single, secure data system that combines chromatography and mass spectrometry into one software platform. OpenLab CDS can control your Agilent GC, LC, single quadrupole GC/MS, LC/MS instruments as well as other vendors’ instruments in the lab, and save substantial time managing software and training staff.
<blockquote>
Presenter: Shawn Anderson (Director of Marketing, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Shawn Anderson is the Associate Vice President of Marketing for the Software and Informatics Division at Agilent Technologies. He has over 28 years of experience in the chromatography and purification/separations market space. His career has developed from bench work in biotech to field support, applications, sales, product management, and product marketing. Shawn is proud to have “learned and led” at some of the most well-respected vendors in the industry such as GE Healthcare, Dionex, Bio-Rad, and now Agilent, designing, launching, and marketing next generation hardware, software and consumable products. He is passionate about end-user and customer voice and believes that the lost sale is often the best learning opportunity. Finally, he is an optimistic futurist, and looks forward to the continuing democratization of science and technology.
</blockquote>

Agilent Technologies: OpenLab CDS-The future of Agilent Chromatography Data System for HPLC, GC, GCMS and LCMS

OpenLab CDS-The future of Agilent Chromatography Data System for HPLC, GC, GCMS and LCMS

Find out about the next generation of software from Agilent.

OpenLab CDS The future of Agilent Chromatography Data System

Two Dimensional HPLC (2D-LC) is a technique where two orthogonal techniques are brought together in one system for the purposes of increasing peak capacity as well as providing additional information from complex mixtures and analytes.
In this presentation we will show the analysis of a mAB with a HIC first dimension followed by a RP/desalting column in the second dimension. The second dimension column will removes salts from the mobile phase which will then allow the eluent to be analyzed by an mass spectrometer without the salts that could cause issues with the MS source.
<blockquote>
Presenter: Bob Giuffre (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Bob Giuffre has been working with HP/Agilent for almost thirty years as an Application Scientist for the HPLC product line. During that time Bob has worked on applications ranging from small molecule pharmaceutics, to large molecule polymers, to monoclonal antibodies. Currently, Bob is working in the area of ultra-high pressure two-dimensional liquid chromatography as a means to increase peak capacity for complex formulations. Bob has authored a chapter on the use of Diode-Array Detection in Pharmaceuticals. He is currently writing a chapter on Troubleshooting Peptide and Proteins Analysis using LC-MS.
</blockquote>
<blockquote>
 Presenter: Sandeep Kondaveeti (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Sandeep is a Philadelphia native and currently works as an applications chemist responsible for developing Bio-pharma workflow applications with Agilent Technologies supporting bio-columns portfolio. Prior to joining Agilent, Sandeep undertook graduate studies at Temple University in Bioinorganic chemistry focused in developing Inorganic metal clusters based on Photosynthetic metalloenzymes and worked as Process Analytical Scientist for Janssen Pharmaceuticals through Eurofins.
</blockquote>

Agilent Technologies: 2D HPLC of Monoclonal Antibody with Novel Hydrophobic Interaction coupled to Reverse Phase Desalting with MS de

2D HPLC of Monoclonal Antibody with Novel Hydrophobic Interaction coupled to Reverse Phase Desalting with MS de

Analysis of a mAB with a HIC first dimension followed by a RP/desalting column in the second dimension.