<div><p><strong>Air bubbles in an HPLC system can disrupt pumping reliability, cause baseline noise, and result in poor result reproducibility and valve function. To prevent these, proper mobile phase degassing is essential.&nbsp;</strong></p><p>Degassing in HPLC not only ensures stable pumping but also improves sensitivity and accuracy by preventing dissolved oxygen from forming bubbles that interfere with detectors such as UV-VIS, fluorescence, refractive index, or electrochemical. Effective HPLC solvent degassing is therefore a critical step for stable, accurate, and reproducible chromatography.</p></div><div><div><div><div><h3><strong>What is HPLC Solvent Degassing and Why is it Necessary?</strong></h3></div></div></div></div><div><p>When solvents such as water, buffers, acetonitrile, and methanol contact the atmosphere, nitrogen and oxygen dissolve into them. When mixed, their combined air content can exceed the solubility of the mixture, creating a supersaturated condition that leads to outgassing and bubble formation. These bubbles disrupt <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc">HPLC</a> performance by causing unstable baselines, bad peak shape, pump cavitation, retention time shifting, and loss of sensitivity.</p><p>In pumps, air can stop flowing or cause erratic retention times, while in optical detectors, bubbles scatter light and generate noise or false signals. Although it is not necessary to remove all dissolved air, degassing in HPLC lowers gas levels below supersaturation and prevents these issues. Low-pressure mixing systems are especially prone to bubble formation, whereas high-pressure mixing reduces outgassing because solvents blend under elevated pressure.</p></div><div><div><div><div><h3><strong>Types of Degassing Techniques in HPLC</strong></h3></div></div></div></div><div><p>Degassing the mobile phase was once a major concern in liquid chromatography with outgassing problems being among the most common issues during the first decades of its use. The introduction of inline degassers in HPLC in the past 15–20 years has significantly reduced the occurrence of these problems, to the point that many users today have little experience with them. However, while inline degassing prevents most solvent outgassing issues, it does not eliminate all problems caused by dissolved air.</p></div><div><div><div><div><h4><strong>Inline Degassing</strong></h4></div></div></div></div><div><p>Inline degassing works by passing the mobile phase through a gas-permeable membrane within a vacuum chamber, allowing dissolved gases to escape while retaining the liquid. Different techniques include:</p><ul><li><strong>Vacuum degasser:</strong> Inline vacuum degassers, now standard in most modern HPLC systems, have greatly reduced bubble-related pump issues by efficiently removing dissolved gases. Solvents pass through porous tubing within a vacuum chamber, where gases diffuse out through micropores to waste, while the degassed liquid continues flowing onto the pump.</li><li><strong>Membrane degasser:</strong> Over the years, various polymer materials have been tried with varying success for degassers. Modern degassers use a thin, highly gas-permeable membrane, making the degassing process very efficient.</li></ul></div><div><div><div><div><h5><strong>Advantages</strong></h5></div></div></div></div><div><p>Inline degassers are highly reliable and effective at reducing dissolved gases in the mobile phase to acceptable levels and are now standard in most HPLC systems. It is usually automatic and trouble-free. They require little to no maintenance, making them convenient and reliable for routine use.</p></div><div><div><div><div><h5><strong>Limitations</strong></h5></div></div></div></div><div><p>Despite their effectiveness, inline degassers do not remove all dissolved air from the mobile phase. The residual air can still cause problems in certain sensitive applications. Membrane replacement, vacuum pump failure, or damaged tubing can also cause issues, so proper maintenance is essential to avoid costly repairs. Additionally, microbial growth in the buffer can block membrane pores and contaminate the entire HPLC system. To prevent microbial growth, contamination, or blockages, it is best not to store the degasser with water or buffer in the tubing and to flush with 30% phosphoric acid followed by water if contamination occurs.</p></div><div><div><div><div><h4><strong>Offline Degassing</strong></h4></div></div></div></div><div><p>Offline degassing involves removing dissolved air from the mobile phase before it enters the HPLC system. The techniques include:</p><ul><li><strong>Helium sparging:</strong> This method requires a solvent inlet filter to prevent particulates and improve gas dispersion, but the degassed solvent can reabsorb air once in the system. To reduce bubble formation, slight back pressure should be maintained, often using a backpressure regulator after the detector. Helium purging can remove <strong>up to 80% of dissolved air</strong>, requiring equal helium volume for organic–aqueous phases. However, the flow rate should be reduced over time to avoid losing volatile components.</li><li><strong>Vacuum filtration:</strong> Vacuum degassing removes <strong>over 60%</strong> of dissolved air from the mobile phase and is performed through batch-wise treatment. It can also be done during filtration, before the chromatographic process with a 0.45 or 0.22 μm membrane filter, though this method is less effective.</li><li><strong>Sonication:</strong> Ultrasonic baths alone are not effective for degassing, as they remove<strong> only about 20-30% of dissolved gases</strong>, while at least 50% removal is needed. However, when combined with helium sparging or vacuum degassing, sonication becomes a <strong>useful complementary method</strong>. Since ultrasonic baths are common in laboratories, they are best applied alongside other degassing techniques.</li></ul></div><div><div><div><div><h5><strong>Advantages</strong></h5></div></div></div></div><div><p>An offline degasser offers the advantage of using a 2 µm solvent inlet filter to prevent particulates from entering the mobile phase while ensuring efficient gas dispersion. It is also cost-effective and flexible, as it relies on standard laboratory equipment and can handle large solvent volumes in a batch.</p></div><div><div><div><div><h5><strong>Limitations</strong></h5></div></div></div></div><div><p>A major drawback of offline helium sparging is that the mobile phase can gradually reabsorb air once placed in the HPLC system, leading to signal loss and changing sensitivity. Additionally, helium sparging can be expensive as it requires specialized gas cylinders.</p><h3><strong>Inline vs Offline Degassing: Side-by-Side Comparison</strong></h3><h5><strong>Timing:</strong></h5><ul><li><strong>Offline Degassing: </strong>It is usually done before the mobile phase enters the HPLC run.</li><li><strong>Inline Degassing: </strong>It is a continuous process that occurs during an HPLC run.</li></ul><h5><strong>Degassing Efficiency:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>The efficiency can be variable. For instance, vacuum/sonication removes 60–70% of dissolved air, sonication alone removes <strong>20–30%</strong> of air and helium sparging removes 80% of air.</li><li><strong>Inline Degassing:&nbsp;</strong>It does not remove 100% of dissolved gas, but it removes most of it. However, it prevents bubble formation.</li></ul><h5><strong>Risk of Re-dissolving:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>The risk is high as solvents can reabsorb air when exposed to the atmosphere.</li><li><strong>Inline Degassing:&nbsp;</strong>The risk is low as the continuous vacuum keeps new gas from dissolving.</li></ul><h5><strong>Cost:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Low initial investment and requires only basic lab equipment. <strong>Helium</strong> sparging can be expensive as it requires specialized gas cylinders.</li><li><strong>Inline Degassing:&nbsp;</strong>Higher initial cost (dedicated degasser unit and vacuum pump).</li></ul><h5><strong>Ease of Use:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Manual setup each time which is time-consuming.</li><li><strong>Inline Degassing:&nbsp;</strong>Automated after installation.</li></ul><h5><strong>Impact on Solvent Composition:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Some loss of volatiles is possible such as evaporation during sparging.</li><li><strong>Inline Degassing:&nbsp;</strong>Solvent composition remains unchanged.</li></ul><h5><strong>Effect on Chromatographic Stability:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Improves initial stability but may allow bubble re-formation later.</li><li><strong>Inline Degassing:&nbsp;</strong>Very stable baselines and retention. Bubble-related problems are virtually eliminated.</li></ul><div><div><div><div><h3><strong>When to Choose Offline vs Inline Degassing</strong></h3></div></div></div></div><div><p>The choice between an offline and inline HPLC degasser depends on the situation, including factors like routine use, troubleshooting needs, and method sensitivity.</p><ol><li>Offline degassing is suitable for occasional use, limited equipment setups, or when routine inline degassing is not available. While inline degassing is preferred for routine analyses requiring consistent performance and high sensitivity.</li><li>Inline degassers have a higher initial cost but save labor. An offline degasser uses low-cost labware and is cheap to set up, though helium or other gases add to the consumable cost.</li><li>If mobile phases contain volatile organics or labile salts, avoid aggressive offline sparging/vacuum (which can evaporate components). Inline vacuum degassing is gentler on the solvent composition.</li><li>Oxygen in the mobile phase is particularly problematic for sensitive methods, such as low-wavelength UV or reductive electrochemical detection. While techniques like helium sparging and inline degassing effectively remove oxygen, remember that offline-sparged solvents are susceptible to reabsorbing air over time, which can lead to signal loss and subtle changes in sensitivity.</li></ol></div><div><div><div><div><h3><strong>Troubleshooting Degassing Issues in HPLC</strong></h3></div></div></div></div><div><h4><strong>1) Cycling of output (pressure or baseline)</strong></h4><p>Cycling patterns in pressure or the chromatogram baseline often indicate poor compressibility at the pump heads. This may result from insufficient degassing efficiency, where adding a second degassing chamber can help. It can also cause air to enter the system due to improperly connected tubing to or from the HPLC solvent degasser. Also, improper connections of the tubing on the HPLC solvent degasser can also allow air to enter the system, causing fluctuations in pressure or baseline.</p><h4><strong>2) Baseline noise</strong></h4><p>Noise on the baseline of a chromatogram may indicate problems with the gas flow system. High gas flow rates can cause short-term noise, while baseline drift (long-term fluctuations) may be due to contaminants being flushed out from a partially clogged gas line. Additionally, an unstable gas flow output can also contribute to baseline noise.</p><h4><strong>3) Leakages</strong></h4><p>Leaks often occur due to incompatibility between solvents and degasser materials. For instance, tetrahydrofuran (THF) can dry and crack degasser membranes. Additionally, improperly seated tubing connections in or out of the degasser can allow leaks to develop.</p><h4><strong>4) Blockages</strong></h4><p>Blockages create pressure ripples as the pump struggles to draw solvent, sometimes causing cavitation. They may result from algae growth, crystallization of buffer salts, or clogged inlet filters. Troubleshooting involves disconnecting outlet tubing to check for continuous solvent flow, while prevention requires filtering solvents, using clean solutions, and flushing lines regularly, especially after system shutdowns.</p><h4><strong>5) General considerations</strong></h4><p>Degasser issues are often linked with the mobile phase quality. Using poorly prepared or contaminated solvents can lead to persistent problems. Maintaining clean, filtered, and fresh solvents helps ensure smooth operation and minimizes downtime.</p></div></div>

Degassing Techniques in HPLC: Inline vs Offline

Everything to know about osmolality

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 13th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you posters by <strong>Agilent Technologies / ASMS, Shimadzu / ASMS, Thermo Fisher Scientific / HPLC Symposium </strong>and<strong> Waters Corporation / HPLC Symposium </strong>and technical note by<strong> KNAUER!</strong></p><h3><strong>1. Agilent Technologies / ASMS: Intact Protein Analysis in Plasma Using Automated 2D-LC/TOF with Multiple Heart-Cutting</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_intact_protein_mhc_2d_lc_tof_wp_679_asms_2026_en_agilent_edb54f4854.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Direct intact and top-down protein analysis enables comprehensive characterization of proteoforms, post-translational modifications, and fragments that are often lost in bottom-up workflows.1 However, intact mass analysis in complex biological matrices such as whole plasma is challenged by extreme dynamic range and ion suppression, commonly necessitating affinity-based enrichment.&nbsp;</p><p>Here, we demonstrate a non-affinity, orthogonal separation strategy using a 2D-LC/TOF workflow with multi-heart-cutting for native intact protein analysis directly in plasma. Therapeutic monoclonal antibodies (mAbs) trastuzumab and antibody-drug conjugate T-DM1, were used as model systems to evaluate performance.&nbsp;</p><p>The workflow employs an Agilent 1290 Infinity III 2D-LC system for orthogonal separations and precise multiple heart-cutting, coupled to a high-resolution, high-sensitivity Agilent 6230C Accurate Mass TOF LC/MS System.</p><h4><strong>Experimental</strong></h4><h5><strong>Instrumentation&nbsp;</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III+2D-LC"><strong>Agilent 1290 Infinity III 2D-LC system </strong></a>comprised the following modules:&nbsp;<ul><li>1290 Infinity III Bio Multisampler with Thermostat (G7137A)&nbsp;</li><li>1290 Infinity III Multicolumn Thermostat (G7116B)&nbsp;</li><li>1290 Infinity III Diode Array Detector (G7117B) with Max-Light cartridge cell (G7117-60020)&nbsp;</li><li>1260 Infinity III Bio Flexible Pump (G7131C)&nbsp;</li><li>1290 Infinity III Bio High-speed Pump (G7132A)&nbsp;</li><li>1290 Infinity Valve Drive (G1170A) with 2D-LC ASM Valve (G5643B)&nbsp;</li><li>Two 1290 Infinity III Valve Drives (G1170A) with multiple heart-cutting valves (G5642-64000) equipped with 40 µL Bio loops&nbsp;</li><li>1290 Infinity III Valve Drive (G1170A) with a 2- position/6-port valve (G4231A) (used as a diverter valve)&nbsp;</li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6230C"><strong>Agilent 6230C Accurate Mass TOF LC/MS System</strong></a></li></ul><h5><strong>LC/MS Analysis&nbsp;</strong></h5><p>Data acquisition and analysis were carried out using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=MassHunter+acquisition"><strong>MassHunter acquisition</strong></a> (12.2), <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=BioConfirm"><strong>BioConfirm</strong></a> (12.1) and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=MassHunter+Qualitative+Analysis"><strong>MassHunter Qualitative Analysis </strong></a>(13.0) software.</p><h4><strong>Conclusions</strong></h4><ul><li>Agilent 2D-LC enables direct native MS analysis of intact proteins from plasma without affinity-based enrichment.&nbsp;</li><li>UV-guided and multiple heart-cutting simplify 2D-LC method development and provide high-resolution isolation of native proteins from complex biological matrices such as plasma.&nbsp;</li><li>The Agilent 6230C Accurate Mass TOF delivers high sensitivity and excellent mass accuracy, enabling confident intact protein characterization under native conditions.</li></ul><h3><strong>2. KNAUER: Mind the pressure in preparative up-scaling – effects of particle size and solvent composition on back pressure</strong></h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0051_knauer_mind_the_pressure_in_preparative_up_scaling_effects_of_particle_size_and_solvent_comp_12e49884d3.pdf"><strong>Full PDF for download</strong></a></li></ul><p>The back pressure of a chromatographic system is generated by the system components, the capillaries, the column, the solvent, and the applied flow rate. Each system has a maximum pressure which it can sustain, and therefore the pressure must be monitored as it is a limiting factor during method development. The back pressure generated by a column is dependent on the particle size and its length. The development of a preparative method is ideally performed on an analytical scale and after optimization the method is then scaled up to preparative scale.</p><p>As preparative HPLC systems normally have lower maximum pressures, it is important to keep the generated pressure in the range of the later preparative system.&nbsp;</p><p>The larger the particles of the column material are, the lower will be the generated back pressure. With increasing particle size, the resolution is decreasing at the same time. Depending on the separation task, the resolution can be a critical point, and it is important to find the right compromise between resolution and backpressure. Additionally, larger particles are significantly less price intensive, which is another factor to be considered especially for large scale purifications. Often solvent mixtures of water with acetonitrile, methanol or ethanol are used for reverse phased methods. Depending on the organic solvent composition, the generated back pressure differs significantly which could be reduced by changing the solvent composition slightly or the organic solvent if possible. The back pressure generated by the solvent will not or only slightly change during the up-scaling process.&nbsp;</p><p>Here, the influence of particle size, flow rate and solvent composition on the back pressure was evaluated. Further, a practical scale-up was performed to evaluate the values obtained on analytical scale to those in larger scale.</p><h4><strong>User Tips:&nbsp;</strong></h4><ul><li>What is the finale scale you want to operate later?&nbsp;</li><li>What are the pressure limitations of the preparative system?&nbsp;</li><li>Be aware of the solvent back pressure&nbsp;</li><li>Consider particle size not only for best separation&nbsp;</li><li>Consider the flow rate</li></ul><h4><strong>MATERIAL AND METHODS&nbsp;</strong></h4><ul><li><strong>Pump: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2372"><strong>AZURA® P 6.1LHPG</strong></a>, 10 ml, sst&nbsp;</li><li><strong>Autosampler: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2375"><strong>AZURA AS 6.1L</strong></a></li><li><strong>Detector:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2423"><strong>UV AZURA DAD 2.1L&nbsp;</strong></a></li><li><strong>Flow cell: </strong>Pressure proof 10 mm, 10 µl, 300 bar&nbsp;</li><li><strong>Thermostat:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2376"><strong>CT2.1&nbsp;</strong></a></li><li><strong>Columns:&nbsp;</strong><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1633"><strong>Eurospher II 100-5 C18, 150 x 4.6 mm&nbsp;</strong></a></li><li>Eurospher II 100-10 C18, 150 x 4.6 mm&nbsp;</li><li>Eurospher II 100-15 C18, 150 x 4.6 mm&nbsp;</li><li>Eurospher II 100-20/45 C18, 150 x 4.6 mm &nbsp;</li></ul></li><li><strong>Software&nbsp;</strong><ul><li>ClarityChrom 10.1&nbsp;</li><li>ClarityChrom 10.1 PDA extension</li></ul></li></ul><h4><strong>CONCLUSION&nbsp;</strong></h4><p>The back pressures of four C18 150 x 4.6 mm columns, which differed only in particle size were measured and compared at different flow rates and solvent compositions. As expected, the 5 µm column showed the highest and the 20/45 µm column the lowest back pressure at all tested conditions. The pressure curves of ethanol and methanol gradients were similar, with a maximum pressure at approximately 50% solvent. The pressure curve of the acetonitrile gradient differed from the other two, showing the highest pressure at a lower organic solvent content of approximately 35%, after which the pressure dropped. Ethanol showed the highest pressure, followed by methanol and then acetonitrile. Determining the column back pressure at an analytical scale is an important tool during preparative method development. Ideally, if a linear scale up from the analytical to the preparative scale is conducted, the column back pressure should stay the same. This helps to predict if the method can be scaled up to the desired size in terms of back pressure.</p><h3><strong>3. Shimadzu / ASMS: Enhanced Quantification of Emerging UV Filter Contaminants in Drinking Water Using Optimized LC-MS/MS Technique</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/TP_121_Enhanced_Quantification_of_Emerging_UV_Filter_Contaminants_in_Drinking_Water_Using_Optimized_LC_MSMS_Technique_e733c64828.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Ultraviolet (UV) filter chemicals from sunscreens and personal care products are increasingly recognized as emerging contaminants of concern in drinking water1 . Their persistence and reported biological effects highlight the need for sensitive and reliable monitoring approaches2 . The objectives were to optimize quantitation to achieve ppt level detection without offline extraction and assess the occurrence of these compounds in multiple drinking water matrices. In this study, 20 UV filters were analyzed using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8060RX"><strong>Shimadzu LCMS8060RX triple quadrupole mass spectrometer.</strong></a></p><h4><strong>Conclusion</strong></h4><p>A highly sensitive, precise, and robust direct injection LC–MS/MS method enables sub-ppt to high-ppb quantification of 20 UV filters in drinking water. Overall, the highly sensitive and robust Shimadzu LCMS-8060RX Triple Quadrupole Mass Spectrometer combined with this extraction-free workflow provides a simple, reliable, and high-throughput approach for quantifying emerging UV filters at ng/L concentrations in drinking water.</p><h3><strong>4. Thermo Fisher Scientific / HPLC Symposium: Comparison of biocompatible and inert UHPLC systems for LC-UV quantitation of large RNA</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004752_hplc_vanquish_amplify_metal_sensitive_analyte_analysis_po004752_na_en_ff99cfde49.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Accurate LC-UV analysis of large RNA and oligonucleotide therapeutics requires highly inert chromatographic flow paths because interactions between the negatively charged phosphodiester backbone and metallic surfaces can cause analyte loss, peak broadening, and reduced quantitative sensitivity, particularly at low concentration.&nbsp;</p><p>Here, an inert UHPLC system – Vanquish Amplify system, incorporating inert-coated flow path components was evaluated against a biocompatible UHPLC configuration using MP35N alloy capillaries and additionally against a commercially available inert UHPLC platform. Large RNA fragments of 500 and 5000 nucleotides were analyzed by IP-RPLC to assess the difference between these systems. To further investigate flow path interactions, adenosine 5′- (α,β-methylene) diphosphate (AMPcP), a metal-sensitive analyte, was analyzed alongside adenosine, a noninteracting reference compound.</p><h5><strong>Material and Methods</strong></h5><ul><li><strong>Instrumentation: </strong>Thermo Scientific Vanquish Amplify UHPLC system</li><li><strong>Column:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=SurePac+Oligo"><strong>Thermo Scientific SurePac Oligo RP MDi HPLC column</strong></a>,<br>2.5 µm 2.1 X 50 mm (P/N: 43712-052132</li><li><strong>Data Analysis: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>The Thermo Scientific Chromeleon Software 7.3.2 MUc Chromatography Data System (CDS)</strong></a> was used for all data acquisition and analysis.</li></ul><h4><strong>Conclusions&nbsp;</strong></h4><p>Using AMPcP with the Vanquish Amplify system demonstrated up to a twofold increase in sensitivity compared to systems with MP35N fluidics under conditions where metal interactions are most pronounced&nbsp;</p><p>For large RNA, the Vanquish Amplify UHPLC system delivered 1.25–1.35× higher signal intensity compared to systems with MP35N fluidics, enabling reduced sample consumption and improved quantitative reliability.&nbsp;</p><p>The Vanquish Amplify UHPLC system demonstrated comparable system inertness under the tested conditions for larger RNA fragments (5,000 nt) and slightly better system inertness for smaller fragments (500 nt) in this study than the MaxPeak HPS containing system, based on peak height and area comparisons using an external UV detector</p><h3><strong>5. Waters Corporation / HPLC Symposium: Development and Transfer of Quantitative Analytical Methods using Universal Detectors</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009493_8cbe4c3cc2.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>Universal detectors such as <strong>Charged Aerosol Detection (CAD)</strong> and <strong>Evaporative Light Scattering Detection (ELSD)</strong> provide valuable alternatives to traditional LC detectors including UV, fluorescence, and mass spectrometry, particularly for analytes lacking chromophores. Although both detectors are suitable for quantitative analysis, they rely on different detection principles—CAD measures the electrical charge transferred to aerosol particles, whereas ELSD detects light scattered by dried analyte particles. Because both detectors exhibit non-linear responses, quantitative methods typically require calibration models such as log-log linear or quadratic regression rather than conventional linear calibration. The poster discusses key considerations for developing robust quantitative methods and highlights the challenges associated with transferring methods between different universal detection platforms.</p><p>To demonstrate detector transferability, the authors migrated a <strong>HILIC method for sugar analysis in fruit juice</strong> from ELSD to CAD. The separation was performed on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/306"><strong>Waters Arc HPLC System</strong></a> equipped with an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=XBridge+BEH+Amide"><strong>XBridge BEH Amide</strong></a><strong> (4.6 × 150 mm, 2.5 μm)</strong> column using a water/acetonitrile gradient containing triethylamine. While the chromatographic conditions remained unchanged, CAD detector parameters were optimized. Both detectors produced highly comparable quantitative performance across a calibration range of <strong>50–1000 μg/mL</strong>, achieving calibration coefficients (R²) above <strong>0.995</strong>, area RSD values below <strong>1.5%</strong>, and nearly identical concentrations of fructose, sorbitol, glucose, and sucrose in an apple juice sample. These results demonstrate that quantitative HILIC methods can be successfully transferred between ELSD and CAD with only detector-specific optimization.</p><p>The poster also evaluates method transfer between different CAD platforms using the <strong>USP Deoxycholic Acid assay</strong>. The method was implemented on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/198"><strong>Waters ACQUITY UPLC H-Class PLUS System</strong></a> with an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1823"><strong>XBridge BEH C18</strong></a><strong> (4.6 × 150 mm, 3.5 μm)</strong> column and compared against a competing LC/CAD system. After scaling the method according to <strong>USP Chapter &lt;621&gt;</strong>, both systems met all USP system suitability requirements, including repeatability and signal-to-noise criteria. Quantitative results for deoxycholic acid and related impurities were virtually identical, confirming that validated CAD methods can be reliably transferred between different vendors' LC/CAD platforms without compromising analytical performance.</p><p>Overall, the study demonstrates that both ELSD and CAD are robust universal detection techniques for quantitative liquid chromatography. While method transfer between identical detector types is generally straightforward, successful migration between ELSD and CAD—or between different CAD systems—requires careful optimization of detector parameters while preserving chromatographic conditions. The presented workflows illustrate that with appropriate calibration and detector tuning, laboratories can achieve equivalent sensitivity, precision, and quantitative accuracy, facilitating method standardization and transfer across analytical platforms.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 29, 2026

Article

Academy

Video

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

The Analytical Scientist

Agilent Technologies

Wiley

Shimadzu Corporation

SCIEX

Waters Corporation

<p><strong>Size exclusion chromatography (SEC) is a noninteractive technique that separates solutes according to their molecular size in non-denaturing conditions. It is often used as the first step in isolation of a protein from a crude sample.</strong></p>
<p>We will discuss the theory behind SEC, overview of mAbs, and characterization requirements; with a focus specifically on the effect of solute size and molecular weight, general rules for using SEC, column selection choices, important mobile phase considerations, and column care. We will explore method development pointers in the troubleshooting section.</p>
<blockquote>
<p><strong>Presenter:</strong> Mohit Patel (Application Engineer, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Mohit Patel is part of the CSD Online AE Team. His primary focus is to support SMLC, Bio-Columns, and consumables.</p>
</blockquote>
<blockquote>
<p>Before joining Agilent, Mohit was an Assistant Professor of Chemistry at Temple University. Mohit developed his passion for science through his experiences as a faculty researcher and a mentor. Mohit actively taught, developed curriculum, and served as senior personnel, with a leadership position, on funded grants, mentored research projects, and co-coordinator of STEAM related outreach services.</p>
</blockquote>
<blockquote>
<p>Mohit has earned a Doctorate degree in Chemistry from Temple University in 2016.</p>
</blockquote>


Agilent Technologies: SEC Overview and Method Development for Biomolecules

SEC Overview and Method Development for Biomolecules

We will discuss the theory behind SEC, overview of mAbs, and characterization requirements.

<p><strong>CHROMATOGRAPHY OF BIOSEPARATIONS 101 WEBINAR SERIES</strong></p>
<p><strong>This webinar series will cover the foundations of chromatography for the separation and analysis of peptides and proteins.  Understanding the fundamentals of bioseparations will enable you to develop methods more efficiently.  At the end of this series, you will be up to date on cutting edge principles and innovations surrounding bioseparations.</strong></p>
<p>Who should attend? </p>
<ul>
<li>Those interested in a refresher on foundational principles and tips for bioseparations  </li>
<li>Those revisiting which columns and mobile phases are optimal for which workflows </li>
<li>Those looking for an overview of industry advancements and new offerings</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Bill Warren (Principal Product Manager – Chemistry Technology Center, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Bill received his Masters in Microbiology and Immunology from University of Louisville School of Medicine. In 1986, he joined Waters Corporation as a senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids. Following four years as a field based technical sales representative for Waters, Bill enjoyed a tenure as Manager, Core Product Evaluation Laboratory within the Waters Worldwide Marketing Organization. Bill currently is a Principal Bioseparation Columns Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of proteins and peptides.</p>
</blockquote>
<blockquote>
<p><strong>HOST:</strong> Zerene Manaloto (Chemistry Products Marketing Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Zerene is a Chemistry Product Marketing Manager within the Americas Field Marketing team. She holds a Chemical Engineering Bachelor’s degree from Stevens Institute of Technology. Early in her career, Zerene was a research scientist at Bristol-Myers Squibb in Process Research &amp; Development involved in preparative scale purification and glass plant operations to support IND and clinical supplies of drug candidates. Prior to joining Waters, Zerene was in consumable field sales, supporting key accounts and customers within the pharmaceutical and clinical industries.</p>
</blockquote>


Waters Corporation: CHROMATOGRAPHY OF BIOSEPARATIONS 101 WEBINAR SERIES

FOUNDATIONS OF REVERSE PHASE SEPARATIONS FOR PEPTIDES AND PROTEINS

This webinar series will cover the foundations of chromatography for the separation and analysis of peptides and proteins.

REVERSE PHASE SEPARATIONS FOR PEPTIDES AND PROTEINS

<p>Key learning objectives</p>
<ul>
<li><p><strong>Solutions through</strong></p>
</li>
<li><p><strong>Liquid Chromatography (LC) Column Technology</strong></p>
</li>
<li><p><strong>Gas Chromatography (GC) Column Technology</strong></p>
</li>
<li><p><strong>Pesticides</strong></p>
</li>
<li><p><strong>Nutraceuticals</strong></p>
</li>
<li><p><strong>Vitamins</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Darshan Engineer (Senior Business Development Manager)</p>
</blockquote>


Phenomenex: Chromatographic Solution for Food, Nutraceuticals & Vitamin Analysis

Chromatographic Solution for Food, Nutraceuticals & Vitamin Analysis

Liquid Chromatography (LC) Column Technology & Gas Chromatography (GC) Column Technology...

Chromatographic Solution for Food, Nutraceuticals & Vitamins

<p>Working with spectral and chromatographic data often results in noisy data. The good news is, there is a simple and efficient way to extract the signals from the noise, enabling a deeper and better understanding of processes and products.</p>
<p>If you are working on product or process development problems, you might need to utilise spectral and chromatographic data to understand and confirm that the process is performing optimally and generates the desired product. Frequently, we may summarise these data by using estimates based on peak height, position, and shape. But using these summaries inevitably causes us to lose some information about our process. This can reduce the insights we gain from our data analysis process and can negatively impact the quality of our decisions.</p>
<p>Spectral and related data are inherently noisy. This webinar will present a simple and efficient way of extracting the signals from the noise for a deeper, better understanding of your processes and products. If you are a scientist or engineer collecting spectral, chromatographic, or similar data and want to gain deeper insight into the trends and outcomes, this webinar is for you.</p>
<blockquote>
<p><strong>Presenter:</strong> Florian Vogt (Systems Engineer JMP, JMP Statistical Discovery)</p>
</blockquote>
<blockquote>
<p>Before joining SAS Institute, Florian worked as a process engineer in research and development at Südzucker AG, where he was involved in process optimization, product development and process evaluation. Florian Vogt holds a Master of Science in Biotechnology.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Emmanuel Romeu (Systems Engineer JMP, JMP Statistical Discovery)</p>
</blockquote>
<blockquote>
<p>Emmanuel is a pre-sales consultant for the chemical industry. Prior to joining JMP, Emmanuel worked for 25 years at Beckman-Coulter &gt; Diagnostic where he successively worked in industrialization, R&amp;D in medical diagnostics and finally as a statistician. He graduated in biological engineering from the University of Clermont-Ferrand and in statistics from the University of Strasbourg.</p>
</blockquote>


Unsplash/Mika Baumeister: Making better use of Spectral and Chromatographic Data in product and process development

Making better use of Spectral and Chromatographic Data in product and process development

This webinar will present a simple and efficient way of extracting the signals from the noise for a deeper, better understanding of your processes and products.

Making better use of Spectral and Chromatographic Data

<p>While the world of R&amp;D is moving towards digitalization, the unique challenges of analytical data management continue to cause difficulties.</p>
<p>In our 2022 Analytical data management survey we learned and confirmed that…</p>
<ul>
<li><p>Analytical data is diverse with multiple techniques, instrument vendors, and software</p>
</li>
<li><p>It is stored and managed in many applications</p>
</li>
<li><p>The majority still find it difficult to access and share</p>
</li>
</ul>
<p>Analytical data is ingrained in numerous research, development, and QA/QC workflows. While a small minority are starting to apply it for data science applications, most struggle to provide easy digital access for its most common uses.</p>
<p>Join our panel of experts as they discuss the results of the 2022 survey; the challenges related to analytical data that cross organizations and industries; and lessons learned through their own experience.</p>
<p>Webinar Learning Objectives:</p>
<ul>
<li><strong>How analytical data heterogeneity can be addressed</strong></li>
<li><strong>Attributes of effectively managed analytical data</strong></li>
<li><strong>Considerations for using analytical data for data science projects</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Nichola Davies (Director Structural Chemistry UK, Oncology R&amp;D, Astra Zeneca)</p>
</blockquote>
<blockquote>
<p>Nichola Davies completed her PhD studies in physical organic chemistry at the University of Cambridge, utilising NMR spectroscopy to investigate positively cooperative binding and the tightening of non-covalent bonds. Nichola then joined Pfizer (Sandwich, UK) as an NMR spectroscopist supporting the medicinal chemistry department, and implemented new techniques for structure elucidation and the application of novel NMR methodologies to support medicinal chemistry design strategies.</p>
</blockquote>
<blockquote>
<p>Having gained further experience in a wider range of analytical techniques, in 2014 she joined AstraZeneca (Cambridge, UK), as an NMR expert within the Oncology Chemistry R&amp;D Unit. Currently, as Director of the Structural Chemistry team, Nichola is delivering analytical techniques to support small molecule structural characterisation, purity and stability assessment, in addition to NMR free ligand conformational analysis and in-cell MS concentration assays.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Mark Kwasnik (Global Product Manager, Analytical Labs, Solvay)</p>
</blockquote>
<blockquote>
<p>Mark Kwasnik is the Global Product Manager for the Analytical Labs at Solvay. In his role, Mark leverages digital tools in R&amp;I to reduce inefficiencies, increase responsiveness for customers. He works to accelerate the growth innovation pipeline by allowing the company’s scientists to access and use the thousands of data sets that are generated in Solvay’s R&amp;I labs.</p>
</blockquote>
<blockquote>
<p>Prior to his transfer to Solvay&#39;s digital department, he worked as an Analytical Lab Manager for the North American R&amp;D Center and as the Global Digital Transformation Manager for the Specialty Polymer business unit.</p>
</blockquote>
<blockquote>
<p>Mark received his B.S. in chemistry and forensic science from the University of Central Florida and a PhD in Analytical Chemistry, with a focus in BioAnalytical Mass Spectrometry, instrumentation development and informatics at the Georgia Institute of Technology.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Graham A. McGibbon (Director, Strategic Partnerships, ACD/Labs)</p>
</blockquote>
<blockquote>
<p>Graham A. McGibbon is currently Manager of Scientific Solutions and Partnerships at Advanced Chemistry Development (ACD/Labs), a role which involves shaping innovative solutions with people in various industries, government organizations, academic institutions and enhancing relationships with organizations that supply analytical instrumentation and/or software.</p>
</blockquote>
<blockquote>
<p>Since obtaining a Ph.D. in Chemistry from McMaster University (Canada) Graham worked in drug discovery research at Boehringer Ingelheim conducting MS analyses of both small and large molecules; and researched modifications and interactions of bioactive small molecules and biopolymers as a faculty member at McMaster. Graham initially joined ACD/labs in 2007 as Product Manager leading the development of mass spectrometry software.</p>
</blockquote>


The Analytical Scientist: How to Overcome the Challenges of Analytical Data Management

How to Overcome the Challenges of Analytical Data Management

The challenges related to analytical data that cross organizations and industries; and lessons learned through their own experience.

<p><strong>The meeting will dive into the topic of Dissolution Testing of semisolid formulations and spotlight on the most relevant instruments/accessories available. We will also discuss some tips and tricks for the method development of semisolids.</strong></p>
<blockquote>
<p><strong>Presenter:</strong> Karen Krauel-Göllner, PhD (Product Manager Dissolution, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Karen Krauel-Göllner is a registered pharmacist and holds a PhD in Pharmacy from the University of Otago, New Zealand. Karen has worked in various roles in the field of pharmaceutical development. For the last 5 years she has been working with Agilent Technologies and currently holds a position as product manager for dissolution systems.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Ken Boda (Dissolution Product Specialist, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Ken joined Varian (now Agilent) in 2005 as an Applications Engineer and is now a Dissolution Product Specialist. Prior to working at Agilent, he worked in pharma specializing in dissolution testing and method development. As a Dissolution Product Specialist, Ken works with customers in determining the best systems for their needs and optimizing their work flows. He has also helped educate customers on dissolution science through webinars, the Dissolution Discussion Group, LinkedIn, and other channels.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Bryan Crist (President, DissoAssist Consulting)</p>
</blockquote>
<blockquote>
<p>Bryan is currently the president of DissoAssist consulting based in Wilmington, NC since 2021. He provides consulting, coaching, and training services for dissolution and drug release in the pharmaceutical industry, specifically, the areas of product and method development and validation, technology transfer, regulatory and compendial compliance, automation, troubleshooting, aberrant data investigation, apparatus and instrument qualification, SOP development, and laboratory audits.</p>
</blockquote>
<blockquote>
<p>Previously, Bryan was a Scientific Affairs Manager with Agilent Technologies located in Cary, North Carolina, USA. His activities from 1997 until 2021 had focused on providing method and technical application support for dissolution and drug release testing in the pharmaceutical industry as well as working with VanKel, Varian and eventually Agilent R&amp;D, Marketing and Sales teams. Prior to 1997, Bryan performed analytical testing, supervised, and managed pharmaceutical laboratories with Bristol-Myers Squibb, Merck, AAI and Abbott Laboratories until he joined the dissolution equipment manufacturer VanKel.</p>
</blockquote>
<blockquote>
<p>Bryan has developed and routinely conducts numerous seminars related to dissolution and drug release testing. He’s provided numerous talks at exhibitions and conferences and has published numerous articles in journals and book chapters on dissolution related techniques and topics. He received his B.S. degree in Chemistry from Atlantic Christian College in Wilson, North Carolina. Bryan has been a former member of the USP Biopharmaceutics Expert Committee and is presently a member of several USP Expert Panels on Performance Verification Testing, Capsules, Performance Tests for Semisolid Dosage Forms and Solubility Criteria for Veterinary Drugs. He’s a Steering Committee member of the AAPS In-Vitro Release Dissolution Testing (IVRDT) Community Leadership Team, co-founder and co-moderator of the Dissolution Discussion Group (DDG) and is a current member of the American Chemical Society.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Vivian Gray (Founder VA Gray Consulting; Managing Director of Dissolution Technologies)</p>
</blockquote>
<blockquote>
<p>Vivian has spent the last 40+ years involved in all aspects of dissolution testing and evaluating new dissolution technology. At the United States Pharmacopeia, she enjoyed a long career serving first as a bench chemist, supervisor and lastly as a liaison to various expert USP committees, including the Biopharmaceutics and Dissolution Expert Committee. In 1997, Vivian joined the DuPont-Merck Pharmaceuticals Company Analytical Research and Development Section as the Head of the Dissolution Group.</p>
</blockquote>
<blockquote>
<p>Vivian has been an invited speaker for conferences on 70 occasions (28 of these were international invitations), lecturing especially in the areas of dissolution method development and validation, FDA requirements in dissolution testing, troubleshooting dissolution method problems, and new dissolution technology and dosage forms. She is a proactive participant in the field as demonstrated by 54 publications, including 6 book chapters. Vivian has co-authored a book on dissolution testing called “Handbook of Dissolution Testing”, Third Edition, published in 2004.</p>
</blockquote>


Agilent Technologies: Revisions to USP chapter 1724 - Dissolution Testing of Semisolids

Revisions to USP chapter 1724 - Dissolution Testing of Semisolids

Join us to get an update on the latest changes and additions to USP chapter <1724>.

Rev. to USP chapter 1724 - Dissolution Testing of Semisolids

<p><strong>The identification of extractables, leachables and chemical migration is a challenge for manufacturers and suppliers of packaging materials, medical devices and food contact materials. E&amp;L analytics is already complex, but it is compounded by legislative and regulatory efforts to recycle plastics. Analytical methods need to screen for not only the known components but also identify degradants and other unexpected compounds. Manufacturers, producers and retailers face the challenge of keeping up with evolving requirements and developing and adapting strategies to reduce plastic waste.</strong></p>
<p>Thus, ways are being explored to incorporate more recycled plastics into products and packaging while maintaining excellent safety, quality and performance characteristics. As they innovate and combine new materials, companies must demonstrate safety and confirm compliance before bringing their products to market. This again increases the demand and importance of highly effective analytics.</p>
<p>Participation is free of charge. Presentations delivered in English.</p>
<p>Register now for this virtual event and share knowledge and challenges with peers and E&amp;L experts.</p>
<p>Who Should Attend?</p>
<p>The webinars are focused on the needs of scientists working in the area of Extractables &amp; Leachables and Food Contact Materials. During these webinars, attendees will learn:</p>
<ul>
<li><strong>Upcoming and new regulations in the field of food, pharma, biopharma and medical devices</strong></li>
<li><strong>Targeted and non-targeted screening workflows by using Waters_Connect</strong></li>
<li><strong>Characterization and structural elucidation of unknowns</strong></li>
<li><strong>Choice of chromatographic techniques, extraction methods and analytical standards &amp; reagents</strong></li>
</ul>
<h3><strong>EPISODE 1 - Virtual European Workshop on Extractables &amp; Leachables for Pharma &amp; Biopharma</strong></h3>
<h5><strong>Presentation: A risk-based assessment approach to extractable/leachable studies of plastic components and systems used in (bio)pharmaceutical manufacturing</strong></h5>
<h5><strong>Presentation: The Pattern Targeting Application</strong></h5>
<p>Register now &amp; join the discussion!</p>
<blockquote>
<p><strong>Presenter:</strong> Lijun Song (Bioprocess Validations Specialist, 3M)</p>
</blockquote>
<blockquote>
<p>Lijun Song is a Senior Validation Engineer at the 3M filtration validation laboratory located in Belgium. After her PhD in Medicinal Chemistry at Ghent University, she joined the 3M validation team in 2019. She focuses on filter compatibility validation and extractable/leachable studies.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Mike Ludlow (Science Lead working in the CMCAnalytical and Materials Science Team, Drug Development Solutions (part of Alliance Pharma Inc))</p>
</blockquote>
<blockquote>
<p>Mike Ludlow is the Science Lead working in the CMCAnalytical and Materials Science Team within Drug Development solutions based in Cambridgeshire, UK (Formerly known as LGC). The team supports an extensive external client base providing a variety of chromatographic and spectroscopic techniques covering both organic and inorganic characterisation. He has over 20 year’s experience in pharmaceutical analysis primarily in the areas of extractables and leachables testing and the identification of impurities such as Nitrosamines in a GxP environment. Before working for DDS, he worked in the Corporate Analytical Function of ICI’s Chemicals and Polymers Division providing support to R&amp;D scientists developing speciality polymer products.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Jeff Goshawk (Applied Informatics, Research &amp; Development, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Jeff holds a BSc degree in Mathematics from the University of Portsmouth and a PhD in Applied Mathematics from the University of Liverpool. He joined Micromass as a software developer in July 2000 and worked on the development of MassLynx and the application managers ChromaLynx, MarkerLynx and MetaboLynx. In 2013 Jeff changed role joining the Waters Scientific Operations organisation as a member of the toxicology group and was exposed to the disciplines of sample preparation and sample acquisition in addition to developing data processing workflows using UNIFI. In 2017 Jeff transitioned into the Applied Informatics group in Scientific Operations to help to solve problems not addressed by mainstream software solutions. It was in this role that development on the Pattern Targeting Application began.</p>
</blockquote>


Waters Corporation: Virtual European Workshops on Extractables & Leachables for Pharma & Biopharma

Virtual European Workshops on Extractables & Leachables for Pharma & Biopharma

Register now for this virtual event and share knowledge and challenges with peers and E&L experts.

Extractables & Leachables for Pharma & Biopharma

<p><strong>The next evolution in separation science is here as you go beyond the proper engineering of an LC system, the primary concern becomes the interaction of your analytes with elements in the instrument flow path. The Waters ACQUITY Premier UPLC System features MaxPeak High Performance Surfaces (HPS) Technology, which offers improvements in separation and detection of chemically complex analytes, enabling you to minimize the risk of undetected analytes – for increased efficiency and confidence in your results.</strong></p>
<p>The ACQUITY Premier system minimizes the analyte loss, improving recovery, and limits of detection. Pairing with any Waters Mass Spectrometer, the ACQUITY Premier system enables you to confidently identify and quantify unknown compounds in complex samples and confirm trace components at the lowest possible levels.</p>
<p>Takeaways from this webinar will be:</p>
<ul>
<li><strong>HPS Technology: Using Surface Science Innovations to Reduce Bias in Chromatographic separations.</strong></li>
<li><strong>Automate workflows from development to manufacturing.</strong></li>
<li><strong>Observing the Impacts of MaxPeak Premier technology in Bioseperations in Peptide analysis, Glycans, and Oligos.</strong></li>
</ul>
<p>Who Should Attend?</p>
<ul>
<li>Lab managers</li>
<li>General manager</li>
<li>Manager</li>
<li>Team lead</li>
<li>Scientist</li>
<li>Analyst</li>
<li>Chemist</li>
<li>Group Leaders</li>
<li>Research associate/ assistant</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Mr. Manimaran U (Asst. Product Manager- Biopharma, Waters India Pvt Ltd - Bengaluru)</p>
</blockquote>
<blockquote>
<p><strong>Moderator:</strong> Ms. Daya (Executive - Marketing Support, Demand Generation, Waters India Pvt Ltd - Bengaluru)</p>
</blockquote>


Waters Corporation: Improving Chromatographic performance to next level with Waters Premier technology

Improving Chromatographic performance to next level with Waters Premier technology

The ACQUITY Premier system minimizes the analyte loss, improving recovery, and limits of detection.

Waters Premier technology

<p><strong>This webinar will demonstrate an LC-MS system that can perform both LC-MS analysis and LC-UV analysis. This single quad has a small footprint that allows it to adapt to different needs.  With the following features, it is hoped that LC-MS will be more commonly used to analyze API, impurities, and perform quality control.</strong></p>
<ul>
<li>A novel deconvolution algorithm named &quot;Mass-it&quot; to identify the peaks</li>
<li>Dual Ion Source (DUIS) for simultaneous ESI/APCI ionization</li>
<li>Artificial intelligence for peak integration of mix samples</li>
<li>Contamination proof robustness</li>
<li>Reliability through automation, starting, cleaning, shutting down, etc.</li>
<li>Six minutes vacuum startup</li>
<li>Less than 3 milliseconds polarity switching</li>
</ul>
<p>We will host a Q&amp;A session after the presentation to address any questions or comments.</p>
<blockquote>
<p><strong>Presenter:</strong> Mridul Mandal, Ph.D. (Market Manager, Shimadzu Scientific Instruments)</p>
</blockquote>
<blockquote>
<p>Mridul Mandal, Ph.D. is currently working as a market manager -pharma/biopharma at Shimadzu Scientific Instruments. Before entering this position, he held numerous roles in academia and pharma CRO for over 12 years. He has published over 30 peer-reviewed publications and book chapters on mass spectrometry, chromatography, and other instrumentation in biomedical science</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Brian Domanski (Strategic Collaboration Scientist, Shimadzu Scientific Instruments)</p>
</blockquote>
<blockquote>
<p>Brian has over 10 years working to provide LC and SFC solutions for Pharma, as well as experience with a wide range of chromatography techniques including RP, NP, HILIC, IEX, SEC/GPC, and IC. His focus also includes scalable Informatics and Data Integrity solutions.</p>
</blockquote>


Shimadzu: Revolutionary Single Quad LC-MS for Drug Development and Quality Control

Revolutionary Single Quad LC-MS for Drug Development and Quality Control

This webinar will demonstrate an LC-MS system that can perform both LC-MS analysis and LC-UV analysis.

Single Quad LC-MS for Drug Development and Quality Control

<p><strong>Unlock new perspectives with our accurate mass 101 webinar series.</strong></p>
<p>Accelerate your workflows and expand your knowledge around accurate mass with these informative webinars.</p>
<p>In this webinar series, SCIEX applications scientists will explain the benefits of using accurate mass, sample analysis, instrument operation and data processing in real-time.</p>
<p>Get the inside scoop and register today!</p>
<h3><strong>Accurate mass 101: fundamentals</strong></h3>
<p>Accurate mass analysis coupled with liquid chromatography is a powerful qualitative and quantitative tool that offers a high degree of specificity, versatility and sensitivity. Make your experience using accurate mass more powerful by attending this webinar to gain a solid understanding of the fundamentals around this technology. We’ll start with an in-depth look at what it is, how it works, acquisition and workflow capabilities, and commonly used terminology. </p>


Accurate mass 101: fundamentals

SCIEX applications scientists will explain the benefits of using accurate mass, sample analysis, instrument operation and data processing in real-time.