<p><strong>Ask any analytical chemist which hyphenated technique has become indispensable over the past decade, and the answer will almost certainly be the same: tandem chromatography–mass spectrometry. Whether it is </strong><a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis"><strong>LC–MS/MS</strong></a><strong> quantifying a novel biologic’s metabolic fate, or </strong><a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-gc-ms/ms-analysis"><strong>GC–MS/MS</strong></a><strong> chasing per- and polyfluoroalkyl substances (PFAS) down to sub-part-per-trillion levels in municipal water, the combination of chromatographic separation with the specificity of tandem mass detection has become the analytical workhorse of choice across virtually every regulated sector.</strong></p><p>Below we examine the key forces propelling this expansion, from biopharma pipeline dynamics and PFAS regulatory mandates to AI-driven automation and reshoring of pharmaceutical manufacturing—and explore what each trend means for laboratories managing sample preparation and analytical workflows.</p><h3><strong>Biopharma: The Engine Room of LC–MS/MS Demand</strong></h3><h4><strong>GLP-1 Agonists and the Metabolomics Gold Rush</strong></h4><p>The explosive commercial and scientific interest in GLP-1 receptor agonists—<a target="_blank" rel="noopener noreferrer" href="https://www.sepscience.com/advancing-glp-1-peptide-analysis-chromatography-challenges-and-innovations-12163"><strong>semaglutide and related peptide therapeutics </strong></a>for diabetes and obesity—has dramatically expanded the analytical demands placed on tandem MS platforms. Discovery programs require precise quantification of peptide drug candidates and endogenous biomarkers across complex biological matrices. These workflows rely on high-sensitivity LC–MS/MS for pharmacokinetic profiling, receptor occupancy studies, and metabolomics characterization. Metabolomics is, in fact, projected to be the fastest-growing application segment in mass spectrometry, driven in part by its growing role in cancer screening, biomarker discovery, and metabolic disease research.</p><p>I have been spending more time learning about GLP-1s because this is the hot topic that people want to discuss on the Concentrating on <a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL?si=n9GLz9zjSCOrVfXPxFPhng&amp;nd=1&amp;dlsi=52868e027aad4d22"><strong>Chromatography podcast</strong></a>. With that said, I was astounded looking at CNBC.com during breakfast this morning to see that <a target="_blank" rel="noopener noreferrer" href="https://www.cnbc.com/2026/03/21/glp-1-diets-restaurants-protein-fiber-weight-loss-drugs.html"><strong>one in eight Americans are currently taking one of these drugs such as Ozempic</strong></a>.</p><h4><strong>Antibody–Drug Conjugates and Gene Therapy: Complexity at Every Step</strong></h4><p>The ascent of antibody–drug conjugates (ADCs) and gene therapies has introduced a new level of analytical complexity. ADCs blend the target specificity of monoclonal antibodies with the cytotoxic potency of small-molecule payloads—and characterizing the linker chemistry, drug-to-antibody ratio (DAR), and conjugate stability demands high-resolution LC–MS/MS at every stage from discovery through QC release testing. Gene therapy vectors require equally rigorous characterization of impurities, aggregates, and empty/full capsid ratios.</p><p>This translates into a direct need for low-evaporation, high-recovery concentration methods that preserve labile protein structures and maintain sample integrity prior to LC–MS/MS injection. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392"><strong>Nitrogen evaporation workflows</strong></a> that minimize oxidative stress and maintain sample temperature are increasingly critical in these pipelines.</p><h4><strong>Precision Medicine and Multi-Omics Integration</strong></h4><p>The shift toward precision medicine has accelerated adoption of high-throughput, multi-analyte MS platforms. Proteomics, metabolomics, and lipidomics workflows are converging on a single analytical platform—tandem MS—and generating datasets of a scale and complexity that were unimaginable ten years ago.</p><p>Make no mistake: the triple quadrupole serves an irreplaceable role in quantitative targeted analysis. At the same time, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=134&amp;manufacturerItems=3"><strong>Orbitrap </strong></a>and <strong>Q–TOF</strong> hybrid platforms are growing rapidly as researchers demand full-scan accurate mass for untargeted discovery.</p><h3><strong>Environmental Mandates: PFAS Testing Drives Adoption</strong></h3><p>Environmental testing is something that every person, chemist or not, can understand the importance of. Mounting mandates from the U.S. Environmental Protection Agency and European regulators regarding PFAS—the “forever chemicals” whose extraordinary environmental persistence is matched only by the analytical challenge of detecting them at trace levels—have created immediate, compliance-driven investment in LC–MS/MS infrastructure.</p><h4><strong>The Regulatory Framework Chemists Need to Know</strong></h4><p>The EPA has codified a suite of LC–MS/MS-based methods as the analytical standard for PFAS in water and solid matrices:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe"><strong>EPA Method 533</strong></a><strong>:</strong> Weak anion exchange SPE combined with LC–MS/MS; covers 25 PFAS including short-chain compounds in drinking water.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination"><strong>EPA Method 537.1</strong></a><strong>: </strong>Reversed-phase SPE followed by LC–MS/MS; covers 18 PFAS including the GenX compound, with method detection limits in the low ng/L range.</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/en/solutions/environmental/water-testing/pfas-in-water/us-epa-method-8327?srsltid=AfmBOooCY1pcavj7B1PH-75d3HO4BGDglNlhmyTyrpDY3zvUksMO1dlg"><strong>EPA Method 8327</strong></a><strong>: </strong>LC–MS/MS for selected PFAS in prepared extracts of aqueous and solid matrices.</li><li><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633"><strong>EPA Draft Method 1633</strong></a><strong>: </strong>The emerging benchmark for PFAS in non-aqueous matrices—aqueous, solid, biosolids, and tissue—quantifying up to 40 PFAS.</li></ul><p>All of these methods designate LC–MS/MS, specifically LC coupled to triple quadrupole or tandem-in-time platforms operating in negative electrospray ionization mode with multiple reaction monitoring (MRM), as the required or recommended analytical technique. PFAS analysis is, at its core, an LC–MS/MS problem.</p><h4><strong>The Sample Preparation Bottleneck</strong></h4><p>Chemists who have worked with PFAS methods understand the particular demands they place on sample preparation. The ubiquity of<a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/tackling-pfas-contamination-practical-insights-from-epa-537.1-method-development"><strong> PFAS in laboratory materials</strong></a>—PTFE tubing, some SPE sorbents, standard bench supplies—means that blank contamination control is as analytically demanding as the detection itself. EPA 537.1 requires that sample volumes of approximately 250 mL be extracted and reconstituted in as little as 1 mL, creating a 250:1 concentration factor that can expose even trace contamination in the sample processing chain. The installation of a delay column between the LC pump and autosampler to prevent instrument background interference is now standard practice.</p><p>This is precisely where robust, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5385"><strong>inert nitrogen evaporation </strong></a>systems earn their keep. PFAS workflows that use nitrogen-assisted evaporation to concentrate extracts prior to reconstitution benefit from materials that avoid PTFE and other fluoropolymers that can leach PFAS background—a consideration Organomation addresses in its concentration system designs.</p><p>The analytical community is also moving beyond targeted PFAS analysis. High-resolution accurate mass (HRAM) spectrometry via Orbitrap technology, paired with non-targeted screening workflows, is gaining traction for the identification of the more than 5,000 currently catalogued PFAS compounds and the novel structures that continue to emerge. LC–MS/MS remains the quantitative gold standard; HRAM provides the discovery layer.</p><h3><strong>Technological Trends Reshaping the LC–MS/MS Workflow</strong></h3><h4><strong>Artificial Intelligence and Automated Data Interpretation</strong></h4><p>The datasets generated by modern tandem MS platforms—particularly in omics applications—are too large and too complex for conventional manual review. AI-driven spectral deconvolution, automated peak integration, and AI-assisted hypothesis generation are now embedded in the software suites accompanying leading instruments. <a target="_blank" rel="noopener noreferrer" href="https://www.agilent.com/about/newsroom/presrel/2022/17feb-gp22004.html?srsltid=AfmBOop3warRIaEojXM2YyMML6RXfMIDzE-Wm0h-SiKkkocBeeET2m7u"><strong>Agilent’s acquisition of Virtual Control’s ACIES </strong></a>software specifically to automate LC–MS and GC–MS data analysis is emblematic of this direction.</p><p>For laboratory managers, AI integration means smaller specialized teams can operate high-throughput multi-analyte workflows—but it also means that the front-end sample preparation must be even more reproducible, since algorithmic interpretation amplifies inconsistencies introduced upstream.</p><h4><strong>Online SPE Coupling and “Chromatography Without Walls”</strong></h4><p>A significant trend is the integration of solid-phase extraction directly into the HPLC flow path—so-called online SPE–HPLC coupling. Systems that combine extraction and chromatographic separation in a single analytical instrument reduce manual transfer steps, minimize the risk of sample loss or contamination, and dramatically increase throughput. For analytes at very low concentrations, online SPE provides the on-column concentration capability that traditional offline preparation achieves with manual evaporation steps.</p><p>That said, offline sample preparation—including <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/5392"><strong>nitrogen-assisted evaporation</strong></a> under controlled conditions, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2407"><strong>liquid–liquid extraction</strong></a>, and protein precipitation—retains significant advantages in flexibility, matrix handling, and the ability to process sample volumes that exceed online system capacity. The two approaches are increasingly complementary rather than competitive in well-designed analytical laboratories.</p><h4><strong>High-Purity Solvents and Reference Materials</strong></h4><p>As detection limits push further into the femtogram range and regulatory methods demand demonstrable blank performance, the quality of analytical consumables—mobile phase solvents, derivatization reagents, reference standards—has moved from background consideration to front-of-mind. Method validation in regulated environments now routinely requires vendor certification of solvent purity at a level that directly interfaces with instrument performance.</p><h3><strong>The Ever-Present Need for Sample Evaporation</strong></h3><p>As LC–MS/MS methods push detection limits lower, the analytical burden shifts increasingly upstream. The quality of the concentration, cleanup, and reconstitution steps preceding injection determines whether the instrument’s theoretical sensitivity is achievable in practice.</p><p><strong>Several specific developments are particularly relevant:</strong></p><ul><li><strong>ADC and gene therapy characterization workflows</strong> require gentle, low-temperature evaporation that preserves protein structure and prevents aggregation prior to LC–MS/MS. Nitrogen evaporation under controlled conditions is the preferred approach.</li><li><strong>PFAS trace analysis</strong> demands evaporation systems constructed from PFAS-inert materials, with rigorous attention to avoiding fluoropolymer surfaces throughout the sample path.</li><li><strong>High-throughput pharmaceutical QC</strong> driven by reshoring demands parallel processing capability—multi-position evaporators that maintain consistent flow and temperature across all positions simultaneously.</li><li><strong>Multi-omics and metabolomics workflows</strong> generate large sample batches that benefit from automated, walk-away evaporation with programmable endpoints to ensure reproducible final volumes across a cohort.</li></ul><p>Tandem chromatography–mass spectrometry is not merely growing; it is becoming structurally embedded in the analytical workflows of every major regulated industry. The convergence of biopharma complexity, PFAS regulatory mandates, AI-enabled data analysis, and pharmaceutical reshoring is creating a multi-year demand cycle that will require laboratories to invest not only in instruments, but in the entire sample preparation ecosystem that makes those instruments perform.</p><p>For analytical chemists, the practical takeaway is this: as mass spectrometry sensitivity continues to advance, the sample preparation step becomes the binding constraint on method performance. Whether you are developing a new PFAS method to meet EPA 1633, characterizing an ADC’s drug-to-antibody ratio, or running a 500-sample metabolomics cohort overnight, the reliability and cleanliness of your concentration and cleanup workflow determines the quality of every data point that follows.&nbsp;</p>

The Continued Rise of Tandem Chromatography–Mass Spectrometry

<h3><span><strong>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</strong></span></h3><p><span><strong>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.</strong></span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"></p><h4><span><strong>1. Improper Filter Handling</strong></span></h4><p><span>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.</span></p><ul><li><span><strong>Solution:</strong> Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</span></li></ul><h4><span><strong>2. Selecting an Inappropriate Pore Size</strong></span></h4><p><span>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.</span></p><ul><li><span><strong>Solution:</strong> A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</span></li></ul><h4><span><strong>3. Choosing the Wrong Membrane Material</strong></span></h4><p><span>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.</span></p><ul><li><span><strong>Solution:</strong> Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</span></li><li><span><strong>Pro Tip:</strong> For additional guidance, see our blog article <strong>"How to Choose the Right Membrane for your Syringe Filter"</strong>, which provides a detailed comparison of membrane materials and their recommended applications.</span></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"></p><h4><span><strong>4. Ignoring Filter Capacity</strong></span></h4><p><span>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.</span></p><ul><li><span><strong>Solution:</strong> When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</span></li></ul><h4><span><strong>5. Overlooking Sample Adsorption</strong></span></h4><p><span>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.</span></p><ul><li><span><strong>Solution:</strong> For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</span></li></ul><h4><span><strong>6. Failing to Optimize Filtration Performance</strong></span></h4><p><span>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.</span></p><ul><li><span><strong>Solution:</strong> If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</span></li></ul><h4><span><strong>Final Thoughts</strong></span></h4><p><span>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.</span></p><p><span>With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627"></p><p><span><strong>Happy filtering!</strong></span></p>

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Metrohm

Agilent Technologies

Thermo Fisher Scientific

Wiley

SCIEX

Waters Corporation

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Virtual Thermo Scientific Proteome Discoverer Software Workshop designed to update users of this software on the newly added features in Proteome Discoverer (PD) 3.2 version.</strong></p><h4><strong>SESSION 1/3</strong></h4><p>Overview of PD 3.2 features, predominantly for novice users: This session should help predominantly novice users to get familiar with basic tasks, such as study creation, default workflows, processing simple data sets and reviewing the results with graphical outputs.</p><p>Participation with the most recent PD 3.2 version (demo license sufficient) is recommended. Demo access to<strong> CHIMERYS™ cloud service</strong> can also be obtained.</p><blockquote><p><strong>Presenter: </strong>Michaela Scigelova (Product Support, Thermo Fisher Scientific)</p><p>Michaela provides customer support to Thermo Scientific Proteome Discoverer software users.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Proteome Discoverer Software Online Workshops

Proteome Discoverer 3.2 training workshop - Session 1

Proteome Discoverer 3.2 workshop (Session 1): new features, study creation, workflows, and results review for novice users.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Virtual Thermo Scientific Proteome Discoverer Software Workshop designed to update users of this software on the newly added features in Proteome Discoverer (PD) 3.2 version.</strong></p><h4><strong>SESSION 2/3</strong></h4><p>In this session, we will explain how quantitation is performed in PD, demonstrate a study setup for different quantitation approaches and conclude by introducing Thermo Scientific TMTpro 32/35 plex lable reagent.</p><p>Participation with the most recent PD 3.2 version (demo license sufficient) is recommended. Demo access to CHIMERYS™ cloud service can also be obtained.&nbsp;</p><blockquote><p><strong>Presenter: </strong>Michaela Scigelova (Product Support, Thermo Fisher Scientific)</p><p>Michaela provides customer support to Thermo Scientific Proteome Discoverer software users.</p></blockquote></div></div></div>

Proteome Discoverer 3.2 training workshop - Session 2

Proteome Discoverer 3.2 workshop (Session 2): quantitation workflows, study setup, and TMTpro 32/35plex overview.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Virtual Thermo Scientific Proteome Discoverer Software Workshop designed to update users of this software on the newly added features in Proteome Discoverer (PD) 3.2 version.</strong></p><h4><strong>SESSION 3/3</strong></h4><p>Dedicated to CHIMERYS™ intelligent algorithm for identification and DIA-based quantitation. We will also discuss individual nodes and parameter settings, concluding with practical tips to help you become a proficient Proteome Discoverer software user.</p><p>Participation with the most recent PD 3.2 version (demo license sufficient) is recommended. Demo access to CHIMERYS™ cloud service can also be obtained.&nbsp;</p><blockquote><p><strong>Presenter: </strong>Michaela Scigelova (Product Support, Thermo Fisher Scientific)</p><p>Michaela provides customer support to Thermo Scientific Proteome Discoverer software users.</p><p><strong>Presenter: </strong>Daniel Zolg (Chief Operating Officer, MSAID)</p></blockquote></div></div></div>

Proteome Discoverer 3.2 training workshop - Session 3

Proteome Discoverer 3.2 workshop (Session 3): CHIMERYS™ algorithm, DIA quantitation, and workflow optimization tips.

<p><strong>Join us for the first in our Small Molecules, Big Impact series, where we introduce the MaxPeak Premier technology - a transformative liquid chromatography technology designed to overcome longstanding challenges in analytical separations. MaxPeak Premier integrates MaxPeak High Performance Surfaces (HPS) Technology, redefining chromatographic performance by minimizing non-specific adsorption, reducing variability, and dramatically improving analyte recovery and sensitivity.</strong></p><p>In this first webinar we will introduce MaxPeak Premier Columns and highlight at a high level what the technology is, how it works, and what products employ this technology. Specific examples will be shown of how the technology can improve separations without diving too deeply into any specific application area. People who routinely experience issues surrounding non-specific adsorption, particularly of acidic analytes would benefit from this webinar. This webinar is especially valuable for discovery scientists who routinely face challenges with non-specific adsorption, such as poor recovery or inconsistent chromatographic behavior of acidic or metal-sensitive analytes.</p><h4><strong>Who should attend</strong></h4><ul><li>Analytical chemists who work with acidic analytes, or have experienced issues with non-specific adsorption. Method development scientists support discovery and research will also get some benefit from this talk.</li><li>This webinar is especially valuable for discovery scientists in small molecule drug discovery and development who routinely face challenges with non-specific adsorption, such as poor recovery or inconsistent chromatographic behavior of acidic or metal-sensitive analytes. Whether you’re in method development, advanced separation science, or routine analysis, you’ll come away with a clear understanding of how MaxPeak Premier can enhance your LC results.</li></ul><h4><strong>Why you should attend</strong></h4><p>Attendees will learn about MaxPeak Premier Technology. We will first start with the issues that MaxPeak HPS addresses, and how they manifest in chromatographic results. Next we will discuss, briefly, the work-arounds that have been employed in the past. Lastly the MaxPeak Premier solution will be re-introduced with a focus on what the technology is, and how it can improve separations by eliminating the secondary interactions between analyte and column/system hardware.</p><h5><strong>In this session, we’ll cover:</strong></h5><ul><li><strong>What MaxPeak Premier technology is: t</strong>he foundational science behind MaxPeak HPS and how it alters surface interactions to reduce unpredictable analyte losses in chromatography.</li><li><strong>How it works: </strong>insights into surface-optimized hardware and column chemistry that mitigate metal-sensitive and other adsorptive interactions without extensive column or system passivation.</li><li><strong>The product landscape</strong>: an overview of MaxPeak Premier columns across chemistries and formats in discovery research, so you can begin to see where this technology fits within your own workflows.</li><li><strong>Practical benefits:</strong> real examples showing improved peak shape, reproducibility, and sensitivity, and how these advantages translate into greater confidence in your data without delving into narrow application niches.</li></ul><blockquote><p><strong>Speaker: </strong>Kenneth Berthelette (Principal Scientist, Waters Corporation)</p><p>Ken earned his Bachelor of Science in Professional Chemistry from Bridgewater State University in Bridgewater, Massachusetts in 2010.</p><p>He joined Waters Corporation in March 2012, where he contributed to multiple product launches as part of the Research and Development team. In 2021, Ken transitioned into a role supporting Chemistry Aftermarket initiatives.</p><p>With a strong focus on applications and customer education, he has delivered numerous seminars and webinars covering topics such as method development, method modernization and transfer, HILIC separation techniques, and LC system troubleshooting.</p></blockquote>

Inert Chromatographic Breakthroughs: Transforming Small Molecule HPLC

How MaxPeak Premier technology improves LC performance by minimizing adsorption, enhancing sensitivity, and ensuring reliable results for challenging analytes.

Transforming Small Molecule HPLC

<p><strong>AI Quantitation software transforms the way you approach compound optimization and HRMS data processing. Powered by advanced machine learning models, it automates previously time‑consuming steps—so you can focus on making decisions, not managing workflows.</strong></p><p><strong>Featured webinar: Introduction to AI Quantitation software for MRM prediction</strong></p><p>This webinar will show you how AI Quantitation software can easily predict MRM transitions for your compounds. You will learn how the software leverages machine learning techniques to create predictive models and how it can be easily trained with your own data to provide improved accuracy for your compound classes. Examine a case study derived from the application of the predictor to a real-world example.</p><blockquote><p><strong>Speaker:&nbsp;</strong>Ismael Zamora</p><p>Ismael Zamora is CEO of Mass Spec Analytica, SL, a company dedicated to the development of software in the field of metabolism and structure elucidation. He participated in the development of the software MetaSite, MassMetaSite, WebMetabase, Shop, MassChemSite and recently AI Quantitation. He is also an associate professor at the Pompeu Fabra University in Barcelona. Ismael Zamora has over 70 publications in journals and books in the field of ADME prediction, medicinal chemistry and drug discovery in general.</p></blockquote>

SCIEX: How AI Quantitation software elevates your workflows

How AI Quantitation software elevates your workflows

This webinar will show you how AI Quantitation software can easily predict MRM transitions for your compounds. 

<p><strong>EQCM-D can be a powerful tool for furthering electrochemistry research in fields diverse as batteries, corrosion and polymer science. &nbsp;In this webinar, co-organized by 3T-analytik, we’ll break down the theory. Then, through some real-world examples show just how researchers across electrochemistry can use EQCM‑D to bring advanced insight into their research by opening a window into real-time mass, viscoelastic and structural changes during electrochemical processes. This webinar is open to all.</strong></p>

Metrohm: EQCM-D: Advanced Insights into Electrochemistry Applications

EQCM-D: Advanced Insights into Electrochemistry Applications

EQCM-D can be a powerful tool for furthering electrochemistry research in fields diverse as batteries, corrosion and polymer science. 

EQCM-D: Insights into Electrochemistry Applications

<p><strong>The analytical separation of therapeutic peptides presents significant chromatographic challenges due to their structural complexity, limited solubility, and the presence of closely related impurities with similar physicochemical properties. Peptides frequently exhibit strong secondary interactions, susceptibility to adsorption on metal surfaces, and a tendency to aggregate or precipitate, all of which demand carefully optimized chromatographic conditions. This seminar outlines practical strategies for peptide analysis with a focus on reversed-phase liquid chromatography (RP-LC), the most widely applied separation mode for peptide characterization and purity assessment.</strong></p><p>Using tirzepatide – a structurally complex GLP-1 and GIP receptor agonist comprising 39 amino acids and a fatty acid side chain – as a case study, a systematic three-step analytical method development approach is demonstrated. Key parameters such as mobile phase composition, gradient slope, pH, temperature, pore size, and stationary phase chemistry are evaluated to achieve high-resolution separation of the target peptide from its impurities. Particular emphasis is placed on the influence of peptide solubility and temperature on chromatographic performance, as well as on the benefits of shallow gradients and optimized flow rates.</p><p>Furthermore, the role of bioinert column hardware is highlighted for sensitive peptides prone to metal-induced adsorption or oxidation, which can result in poor peak shapes, reduced recovery, and compromised quantitative reliability. Advanced hybrid stationary phases and bioinert coated hardware are shown to significantly improve peak symmetry, sensitivity, and robustness.</p><p>Overall, this seminar demonstrates that strategic method development combined with appropriate column chemistry and inert system components is essential for reliable, high-resolution peptide analysis in modern (U)HPLC workflows.</p><blockquote><p><strong>Speaker:</strong> Dr. Daniel Eßer (Product Manager Analytical Chromatography, YMC Europe GmbH)</p></blockquote>

Wiley: Analysing GLP-1 Peptides with Strategic Precision

Analysing GLP-1 Peptides with Strategic Precision

Explore RP-LC method development for therapeutic peptides, including tirzepatide analysis, bioinert hardware, and optimized chromatographic conditions.

<h4><strong>Tackle Real Workflows for Complex Bioanalysis Challenges</strong></h4><ul><li>Working in DMPK?</li><li>Curious how modern software is making workflows for new modalities easier?</li></ul><p>We’re excited to invite you to DMPK in Action, a new 3-part webinar series designed for &nbsp;bioanalysis scientists that brings real workflows to your screen through practical demonstrations of our system and software.</p><h5><strong>See real DMPK workflows in action - designed for the challenges of modern, complex therapeutics.</strong></h5><p>In short 30 min sessions, we’ll show real workflows, with a focus on practical applications, efficiency, and how DMPK scientists can move from sample to result with greater confidence.</p><h5><strong>Episodes</strong></h5><ul><li>Explore the DMPK workflow in action</li><li>From Sample to Result</li><li>Data handling and analysis with confidence</li></ul><h5><strong>Why you should attend</strong></h5><p>Each session shows how our system and software support practical DMPK tasks, improve visibility, and help teams work more efficiently.</p><h5><strong>You'll see:</strong></h5><ul><li>Real DMPK workflow demonstrations.</li><li>Practical use cases for bioanalysis scientists.</li><li>How our system and software support consistency, reliability, and efficiency.</li></ul><h5><strong>Who should attend</strong></h5><p>If you’re involved in DMPK, bioanalysis, or ADME workflows, this series is designed to give you a clear look at how the process works in practice, delivered straight to your office</p><p><i>Can't attend the webinars live? Register today and watch the episodes on-demand at your convenience.</i></p><blockquote><p><strong>Speaker:</strong> Antonia Wierzbicki (Principal MS Market Dev Manager, EMEA, Waters Corporation)</p><p>Antonia Wierzbicki is a MS market development manager at Waters working in EMEA. Antonia has BSc in Pharmaceutical Science and a MSc in Cancer Pharmacology.</p><p>Following several years working as an analytical chemist in drug development roles within both academic labs and contract research organizations, Antonia has more recently spent time working with analytical chemists to provide solutions using cutting edge technologies to meet their needs and requirements, with a specific interest in ultra-sensitive quantitation of small molecules.</p><p>Antonia is based in Cambridge UK and keen to connectwith like-minded scientists in the field.</p><p><strong>Speaker:</strong> Henry Foddy (Associate Scientist, Waters Corporation)</p><p>Henry is a Scientist in the Routine Mass Detection Team at Waters, working on applications to support Waters’ single quadrupole portfolio that span a variety of market segments. He has almost 3 years of experience with Waters, working previously in a food and environmental role support regulatory testing of Pesticides, PFAS, and Pyrrolizidine Alkaloids. Prior to joining Waters, Henry obtained an MSc in Neuroscience from King’s College London.</p></blockquote>

Explore the DMPK workflow in action

Explore real DMPK and bioanalysis workflows, from sample to result, with practical demonstrations of software, data analysis, and MS-based solutions.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Are you tired of dealing with inconsistent LC results? Are you looking to extend the life of your LC columns and improve lab efficiency? This webinar which was recently broadcasted in APAC, is designed to help you get the most out of your LC columns.</strong></p><h5><strong>Our expert will share invaluable insights on:</strong></h5><ul><li>Understanding LC column chemistry to optimize separations</li><li>Proper handling of new LC columns for maximum lifespan</li><li>Proven techniques to extend column life and reduce downtime</li><li>Effective column maintenance and troubleshooting strategies</li></ul><p>This webinar is an excellent opportunity to deepen your knowledge, improve your lab efficiency, and reduce operational costs. Whether you're a seasoned pro or just getting started, you'll walk away with practical knowledge to elevate your column performance and lifetime. We scheduled time at the end to answer your questions.</p><h5><strong>Who Should Attend?</strong></h5><ul><li>Laboratory Analysts and Scientists who are involved in small molecule pharmaceutical analysis</li><li>Chromatographers seeking to optimize LC column performance</li><li>Quality control professionals aiming to improve data reliability and reproducibility</li><li>Research and development scientists working on method development and validation</li><li>Lab Managers: Leaders looking to improve operational efficiency and extend the lifespan of lab equipment.</li><li>Anyone involved in LC column selection, maintenance, and troubleshooting</li></ul><blockquote><p><strong>Presenter: </strong>Adham Ahmed (Global Product Manager, Thermo Fisher Scientific)</p><p>Adham Ahmed has 15+ years of experience in particle development and functionality for chromatographic application. Presently, he is the Global Product Manager of the Hypersil BDS and ODS and other columns within the Thermo Fisher Scientific Portfolio. His experience includes working in R&amp;D, application specialist and Product management, with a focus on understanding customer’s challenges and laboratory needs.</p></blockquote></div></div></div>

Thermo Fisher Scientific: LC columns tips and tricks

LC columns tips and tricks

This webinar is designed to help you get the most out of your LC columns.

<p><strong>Furanocoumarins are natural compounds found in certain plants, including citrus fruits, parsley, celery, and carrots. Citrus oils in particular, are sometimes used in skincare products to effectively remove bacteria, dead skin cells, and irritants to improve overall skin health, address common issues like acne and provide a pleasant scent. *1 &nbsp;However, they can pose risks when exposed to ultraviolet (UV) light.</strong></p><p>Phytophotodermatitus (PPD) is a reaction that is entirely independent of the immune system. The development of PPD is dependent upon exposure of the skin to a photosensitizing substance (furanocoumarin) and UV radiation, especially in the UV range of 320-400 nm. When a photon of the appropriate wavelength strikes a furanocoumarin, the molecule is raised to a triply exited state from the ground state energy. Once the molecule returns to the ground state, energy is released in the form of heat, fluorescence or phosphorescence which may result in a photoproduct being formed. *2</p><p>The chemical structure of furanocoumarins consists of a furan ring fused with a coumarin. The furan ring, fused in various ways, produces several different isomers. The parent compounds of the most common isomers are psoralen and angelicin. Derivatives of these two compounds are referred to respectively as linear and angular furanocoumarins,*3 since &nbsp;they exhibit a linear or angular chemical structure.</p><p>The LC/MS method outlined here has been shown to be linear, accurate and precise for the characterization and quantitation of Thirteen common furanocoumarins in citrus oils.</p>

Agilent Technologies: Makeup of Your Make-up

Measure your Furanocoumarins like a Pro with the Agilent Pro iQ

Discover an LC/MS method for the characterization and quantification of 13 furanocoumarins in citrus oils used in cosmetics and personal care products.