<p><strong>Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “</strong><i><strong>Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA</strong></i><strong>”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.</strong></p><h5><strong>Could you describe your project and its main objectives?</strong></h5><p>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.</p><p>Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.</p><h5><strong>Why is this export of unspliced ​​RNA so important for retroviral replication?</strong></h5><p>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.</p><p><strong>Simply put: </strong>the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.</p><h5><strong>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</strong></h5><p>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the<strong> NIH </strong>and <strong>Rockefeller University</strong>, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.</p><h5><strong>How will this research contribute to the development of new treatments for retroviral infections?</strong></h5><p>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.</p><h5><strong>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</strong></h5><p>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from <strong>NIH, Frederick</strong>, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from <strong>Rockefeller University to employ iCLIP </strong>methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.</p><h5><strong>Who else is collaborating on the project?</strong></h5><p>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of<strong> Organic Chemistry and Biochemistry of the CAS (IOCB Prague)</strong> and the <strong>Central European Institute of Technology in Brno (CEITEC)</strong>. Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.</p><h5><strong>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</strong></h5><p>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.</p><h5><strong>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</strong></h5><p>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.</p>

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

News from LabRulezLCMS Library - Week 20, 2026

<p><strong>🎤Lindsay Repka&nbsp;</strong></p><p><strong>Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.</strong></p><p>In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.</p><p>What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.</p><p>This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.</p><p><strong>If you work in:</strong></p><ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul><p>…this conversation will resonate.</p><h4><strong>Key Topics Covered</strong></h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3><strong>Video Transcription</strong></h3><p>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.</p><h4><strong>What is photoredox chemistry?</strong></h4><p>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.</p><p>Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.</p><h4><strong>Photocrosslinking and drug-target interactions</strong></h4><p>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.</p><p>Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.</p><h4><strong>From photocrosslinking to solvent activation</strong></h4><p>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.</p><p>The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.</p><h4><strong>Why LC-MS was critical</strong></h4><p>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.</p><p>Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.</p><h4><strong>Photocatalyst stability workflow</strong></h4><p>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.</p><p>Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.</p><h4><strong>Quantifying catalyst remaining by LC-MS</strong></h4><p>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.</p><p>Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.</p><p>Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.</p><h4><strong>Why GC-MS was used later</strong></h4><p>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.</p><p>Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.</p><h4><strong>Practical lessons from the project</strong></h4><p>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.</p><p>She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.</p><h4><strong>Final perspective</strong></h4><p>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

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Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Tosoh Bioscience

Metrohm

Mettler-Toledo

Bruker

Phenomenex

Thermo Fisher Scientific

Shimadzu Corporation

Evosep Biosystems

Waters Corporation

<div><p><strong>As demand for glucagon-like peptide-1 (GLP-1) drugs continues to grow, ensuring these products meet regulatory standards while delivering fast, reliable results is critical. Traditional amino acid analysis (AAA) workflows offer valuable quantitative insights into bioprocess conditions during production but are often time-consuming and complex.</strong></p><p>In this webinar we will introduce a streamlined, microwave-assisted UPLC approach designed to rapidly hydrolyze, label, and identify GLP-1 analogs using the Waters AccQ·Tag Ultra Chemistry Kit. Discover how this turnkey solution expedites amino acid analysis while maintaining accuracy and reliability in conjunction with Empower™ Chromatography Data System (CDS) workflows.</p><h4><strong>Why this webinar matters</strong></h4><ul><li>Learn how to accurately assess GLP- 1 analog composition and quantification using AAA workflows.</li><li>Understand the benefits of rapid derivatization and the simplicity of AccQ·Tag Ultra Chemistry Kits.</li><li>Discover how Empower™ Chromatography Data System (CDS) minimizes errors through automated workflows and custom fields</li></ul><p>This webinar is designed for scientists and managers in Quality Control who work with the analysis or testing of critical quality attributes (CQAs) of peptides, proteins, and especially GLP‑1 analogs.</p><p><i>Don't miss it.</i></p><blockquote><p><strong>Presenter:</strong> Duanduan Han, Ph.D. (Senior Scientist, Waters Corporation)</p><p>Duanduan Han, Ph.D., is a Senior Scientist at Waters Corporation in Milford, MA. Her work has focused on LC/MS method development and troubleshooting, impurity profiling, raw material screening of GLP-1s, lipid nanoparticles, surfactants, oligonucleotides, mAbs, and other biologic drug products.</p><p>She received her Ph.D. in chemical engineering from Texas A&amp;M University.</p><p><strong>Presenter:</strong> Tim Anderson, Ph.D. (Senior Product Manager, Waters Corporation)</p><p>Tim Anderson, Ph.D., has received advanced degrees in Analytical Chemistry and Food Science related to extraction and MS analysis of proteins at Iowa State University. He has industry experience with large manufacturing product analysis and fit-for-use testing.</p><p>Tim has been at Waters working with consumables for 3 years and is Senior Product Manager for AccQ·Tag Ultra solutions.</p></blockquote></div>

Rapid Analysis of GLP-1 Analogs Using Pre-Column Derivatization

Discover microwave-assisted UPLC amino acid analysis workflow for accurate GLP-1 analog characterization using AccQ·Tag Ultra chemistry and Empower CDS.

GLP-1 Analogs analyis by Pre-Column Derivatization

<div><h4><strong>Navigating Challenges and Opportunities for Environmental Testing Labs Under the Revised EU Water Framework Directive</strong></h4><p><strong>The European Commission’s proposed updates to the Water Framework, Groundwater, and Environmental Quality Standards Directives are bringing new responsibilities and opportunities for environmental testing labs. With expanded pollutant lists - including PFAS, pharmaceuticals, and pesticide metabolites - and stricter monitoring and reporting requirements, the landscape of water analysis is rapidly evolving.</strong></p><p>Join the 4th Waters Environmental Webinar Series to explore how your lab can navigate these challenges and turn regulatory change into opportunity.</p><p>Register for free and partner with us to shape the future of European water quality.</p><h5><strong>Why Attend</strong></h5><p>Stay ahead of new EU regulatory demands with insights from leading experts. Gain practical guidance, share experiences with peers, and prepare your lab for the next generation of water quality standards.</p><h5><strong>Highlights</strong></h5><ul><li>New pollutant categories and analytical challenges</li><li>Updates to monitoring protocols and reporting</li><li>Innovative methods to meet revised Environmental Quality Standards</li><li>Strategic guidance for compliance and adaptation</li></ul><p><strong>Lead the change in water analysis—stay ahead, stay compliant, and make a real impact.</strong></p><h4><strong>EPISODE 3: Master the challenging EQS values for endocrine active compounds</strong></h4><h4><strong>What you Will Learn</strong></h4><ul><li>How a sensitive LC-MS/MS supports the detection of estrogens in different water types</li><li>How to develop a method for BPA and further bisphenols in environmental samples</li></ul></div>

Master the challenging EQS values for endocrine active compounds

How a sensitive LC-MS/MS supports the detection of estrogens in different water types

Challenging EQS values for endocrine active compounds

<div><p><strong>Join us for an exclusive webinar featuring two global pioneers in spatial chemical analysis: Jonathan Sweedler from the University of Illinois Urbana-Champaign and Carsten Hopf from the Center for Mass Spectrometry and Optical Spectroscopy (CeMOS) at Mannheim University.</strong></p><h4><strong>What you will learn:</strong></h4><ul><li>How advanced multimodal imaging workflows enable highly specific ROI-focused molecular imaging in complex tissues.</li><li>How combining QCL-based IR microscopy, fluorescence microscopy and MALDI Imaging allows rapid feature detection, and deeper molecular analysis.</li></ul><h4><strong>Who should join:</strong></h4><ul><li>Experienced MALDI Imaging users aiming to improve spatial specificity, molecular identification confidence, and sample throughput.</li><li>Scientists and researchers interested in multimodal imaging workflows that integrate IR and fluorescence microscopy to guide targeted MALDI Imaging.</li></ul><h4><strong>Topics covered:</strong></h4><ul><li>New MALDI Imaging strategies for ROI-specific molecular analysis.</li><li>Multimodal integration for rapid tissue screening and biological feature labeling.</li></ul><blockquote><p><strong>Presenter:</strong> Carsten Hopf, Ph.D.</p><p>Carsten Hopf is a Professor of Bioanalytics and Drug Discovery at Mannheim University of Applied Sciences and the Director of the Center for Mass Spectrometry and Optical Spectroscopy (CeMOS). A leader in MALDI Imaging, his work focuses on advancing various imaging modalities to map the spatial distribution of drugs, lipids, and proteins in tissues and cells for complex diseases, such as cancer and Alzheimer's.&nbsp;</p><p><strong>Presenter:</strong> Jonathan Sweedler, Ph.D.</p><p>Jonathan Sweedler holds the Chair in Chemistry at the University of Illinois Urbana-Champaign and is the Editor-in-Chief of Analytical Chemistry. He's a pioneer in analytical neurochemistry, focusing his research on developing ultrasensitive mass spectrometry and imaging tools to uncover the chemical secrets of individual cells and complex tissues.&nbsp;</p></blockquote></div>

Bruker: MALDI Imaging Becomes IR-resistible: Going Multimodal for Advanced Biomedical Insights

MALDI Imaging Becomes IR-resistible: Going Multimodal for Advanced Biomedical Insights

Webinar on multimodal imaging combining MALDI, IR, and fluorescence microscopy for precise ROI-based molecular analysis in tissues.

MALDI Imaging Becomes IR-resistible

<h4><strong>Scalable workflows are no longer a nice‑to‑have in proteomics, they’re essential.</strong></h4><p>In this upcoming webinar series, we explore how standardized, automated proteomics workflows are enabling reproducible results at scale—from discovery to translational and applied research. Hear directly from experts and users as they share practical insights on building robust, high‑throughput workflows that keep pace with growing sample volumes, increasing complexity, and real‑world laboratory demands.</p><p>Join us to learn how scalable proteomics workflows are moving from promise to practice.</p><h5><strong>Automated Mag-Net Enrichment Unlocks Deep and Cost-Effective LC-MS Plasma Proteomics</strong></h5><ul><li>Salla Keskitalo, PhD, LSRI Director Viikki Proteomics Unit, University of Helsinki</li></ul><p>Plasma is an ideal material for proteomics due to its diverse protein content reflecting physiological and pathological states, and its compatibility with minimally invasive sampling. Deep proteomic profiling of plasma is limited by high-abundant proteins that mask the detection of low-abundant proteins. To overcome this, we compared five plasma protein enrichment methods, Mag-Net, ENRICHplus, ENRICHiST, EasySep, and EXONET, against neat plasma using LC-MS proteomics. All five methods substantially increased protein identifications, with Mag-Net, ENRICHplus, EasySep, and EXONET yielding up to 4200 proteins per sample, over 7-fold more than neat plasma, using a 44-minute gradient on the Evosep One and data-independent acquisition on the timsTOF Pro 2. These methods enriched extracellular vesicle-associated proteins while effectively depleting high-abundant proteins. To further enhance performance and scalability, we optimized the Mag-Net protocol by increasing the plasma-to-bead ratio and automated the workflow, including Evotip loading, on the Biomek i5 liquid handler. The automated Mag-Net, combined with the Orbitrap Astral mass spectrometer, yielded up to 4500 proteins per sample with a throughput of 100 samples per day. The automated Mag-Net enrichment strategy enables affordable, scalable, high-throughput LC-MS plasma proteomics, supporting biomarker discovery across large cohorts.</p><h5><strong>Topic to be announced</strong></h5><ul><li>Joachim Smollich, PhD Student, Faculty of Biology, Department of Molecular Cell Biology, Weizmann institute of science</li></ul>

Evosep: Scalable Workflows for standardized proteomics

Scalable Workflows for standardized proteomics

Join us to learn how scalable proteomics workflows are moving from promise to practice.

<h3><strong>Demonstration of DirectInject-LC and iC LC</strong></h3><ul><li><strong>DirectInject-LC technology overview</strong></li><li><strong>How to run an experiment with DirectInject-LC</strong></li><li><strong>Transforming HPLC into online PAT</strong></li></ul><p>Calum Welsh of METTLER TOLEDO provides an overview of DirectInject-LC™ - how it works and how to run an experiment. DirectInject-LC™ transforms HPLC into an online technique for reaction analysis that enables scientists to gain the data required to understand complex processes in near real-time.</p><blockquote><p><strong>Presenter:</strong> Calum Welsh (Reaction Analysis Group, METTLER TOLEDO)</p><p>Calum Welsh is an accomplished professional with extensive experience in the field of chemistry and product management. Currently serving as a Product Manager for Reaction Analysis at Mettler-Toledo International, Inc. since July 2019, Calum has also held the role of Technology and Application Consultant for AutoChem. Calum completed his Ph.D. and postdoctoral fellowship at The University of Nottingham.</p></blockquote>

Mettler Toledo: Online HPLC Analysis with DirectInject-LC

Online HPLC Analysis with DirectInject-LC

DirectInject-LC turns HPLC into online PAT for real-time reaction monitoring and process understanding.

<p><strong>When titration results fall out of specification, resolving the chemistry is only part of the solution. In regulated pharmaceutical laboratories, it is equally critical to demonstrate control over the workflow that produced the result.</strong></p><p>Unexpected OOS (out of spec) often stem from subtle but significant factors like electrode performance issues, matrix interferences, or poorly optimized Karl Fischer parameters. In this case study focused mini course, Eduardo Simões, Titration Product Specialist, walks through the most frequent pitfalls encountered in potentiometric and Karl Fischer analyses and demonstrates practical, standards aligned strategies to correct them at the bench.</p><p>Building on these corrective actions, Martin Kobler, Senior Product Specialist for Software, demonstrates how compliant digital controls ensure those improvements are consistently applied and defensibly documented. Through structured user roles, controlled method versioning, secure review workflows, and a comprehensive audit trail, participants will see how software transforms troubleshooting into a repeatable, inspection ready process.</p><p>Participants will gain a clear framework for preventing recurring deviations, strengthening data integrity, and maintaining inspection-ready titration workflows. Stick around for a live Q&amp;A to explore how these strategies apply to your own titration challenges.</p><h4><strong>Please plan to attend if you want to: </strong></h4><ul><li>Identify KF and potentiometric failure modes and apply compendial‑aligned corrective actions</li><li>Connect analytical troubleshooting steps to compliant software controls and audit trail documentation Implement best practices that improve method robustness and consistency in a GxP environment</li></ul><h4><strong>Key Learning Points</strong></h4><ul><li>Identify the primary causes of out-of-specification titration results.</li><li>Apply practical, standard-aligned corrective actions for potentiometric and KF analyses.</li><li>Understand how digital controls strengthen data integrity through role management, version control, and secure review workflows Learn how software tools create defensible, audit‑ready documentation.&nbsp;</li></ul><blockquote><p><strong>Presenter: </strong>Martin Kobler (Sr. Product Specialist, Software, Metrohm USA)</p><p>Martin Kobler is the Senior Product Specialist for Software at Metrohm USA where he supports the OMNIS platform and its integration across laboratory environments. Based in Riverview, Florida, he works within the Marketing – Product Management department and collaborates closely with product teams, technical specialists and customer-facing groups to guide software adoption and ensure users can fully leverage OMNIS capabilities.</p><p>With extensive experience supporting Metrohm’s digital solutions, Martin provides expert instruction on software functions, compliant workflows and method execution. His background includes deep familiarity with customer needs and hands-on guidance that helps laboratories transition smoothly to modernized, secure and audit-ready software ecosystems. He is frequently engaged in internal training, live demos and cross-functional initiatives to strengthen software proficiency across the organization.</p><p>He is based in Riverview and can be reached through his Metrohm USA contact channels.</p><p><strong>Presenter: </strong>Eduardo Simões (Titration Product Specialist, Metrohm USA)</p><p>As the Titration Product Specialist at Metrohm USA in Riverview, Florida, Eduardo Simoes, MSc, leverages 22 years of practical experience in potentiometry and electroanalysis. His expertise spans instrument installations, technical support, and training, gained through extensive field work. He holds bachelor's and master's degrees in analytical chemistry from the University of Campinas, Brazil, providing a strong theoretical foundation for his hands-on approach.</p></blockquote>

Metrohm: From Out-of-Spec Results to Audit-Ready: Controlling Titration in GxP Labs

From Out-of-Spec Results to Audit-Ready: Controlling Titration in GxP Labs

Master KF and potentiometric titration troubleshooting and discover how software workflows improve data integrity, method robustness, and audit readiness.

Controlling Titration in GxP Labs

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Struggling to keep up with tightening PFAS regulations and shrinking reporting limits? Laboratories are under increasing pressure to deliver faster, more accurate results while managing complex environmental samples. In this Digital Classrooms session, explore a comprehensive approach to PFAS analysis that combines Shimadzu's total solution with an innovative strategy to tackle these challenges effectively.</strong></p><p>This session covers Shimadzu's PFAS capabilities across its analytical platforms — delivering precise quantification and broad-spectrum characterization across different PFAS classes, while MALDI-TOF imaging accelerates rapid screening, allowing labs to triage high-risk samples in minutes while protecting critical LC-MS/MS systems from contamination.</p><p>Whether you work in environmental testing, regulatory compliance, water quality, or research, this session provides actionable insights to improve your PFAS testing capabilities. Join us to stay ahead in the ever-evolving world of PFAS analysis. Register Now!</p><h4><strong>Learning Objectives</strong></h4><ul><li>Understand how advanced LC-MS/MS workflows enable sensitive and selective quantification of regulated PFAS compounds in environmental matrices, including strategies to meet tightening reporting limits and evolving regulatory requirements.</li><li>Recognize the value of rapid MALDI-TOF imaging as a high-throughput screening tool for identifying elevated PFAS levels in water samples, enabling efficient sample triage and reducing contamination risk to LC-MS/MS systems.</li><li>Evaluate how integrating screening and confirmatory mass spectrometry approaches improves laboratory efficiency, data confidence, and regulatory readiness in modern PFAS monitoring programs.</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Ruth Marfil-Vega (Manager, Sales Headquarters, Global Co-Creation Sales Strategy Office, Shimadzu Corporation)</p><p><strong>Presenter: </strong>Caroline Jones (Global MALDI Applications Specialist, Kratos Analytical, Shimadzu Corporation)</p></blockquote></div></div></div>

Shimadzu: Environmental PFAS Testing: Introducing Next-Generation Mass Spectrometry Solutions

Environmental PFAS Testing: Introducing Next-Generation Mass Spectrometry Solutions

Explore advanced PFAS analysis strategies combining LC-MS/MS and MALDI-TOF screening.

PFAS Testing: Next-Generation Mass Spectrometry Solutions

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Looking to further elevate your Thermo Scientific Chromeleon CDS skills? Want to learn more about the administration tools for your Chromeleon software setup? Already attended </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5981"><strong>Administration Console I webinar (tips, shortcuts, and user/access management)</strong></a><strong>? Part II is the next step – focused on system-level management, structured resources, and automation across your Chromeleon software environment.</strong></p><p>In this practical session, we’ll dive into more Administration Console capabilities that help you manage and standardize your setup at scale – from Data Vault configuration and Chromeleon Resources to eWorkflows and automated rollout of updates. You’ll leave with proven approaches for keeping multi-station environments consistent, controlled, and easier to support.</p><h4><strong>What’s different in Part II (vs. </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5981"><strong>Part I</strong></a><strong>):</strong></h4><ul><li><strong>Beyond users &amp; permissions:</strong> Shift from access management to managing Chromeleon Resources and system components.</li><li><strong>System structure &amp; governance: </strong>Learn how Data Vaults support secure, controlled data handling and standardized operations.</li><li><strong>Remote administration: </strong>Explore methods for remotely updating Chromeleon Stations and reducing manual touchpoints.</li><li><strong>Real-world best practices: </strong>Practical guidance to help maintain stability, security, and compliance as environments grow.</li></ul><h4><strong>Who should attend:</strong></h4><p>This session is designed for anyone wanting to learn more about administration of Chromeleon CDS, administrators, lab IT/support teams, and power users who want to take on administration responsibilities – especially those supporting multiple stations or managing standardized deployments.</p><p>Get practical, immediately usable techniques to keep your Chromeleon software setup <strong>scalable, consistent, and supportable</strong> – with less manual effort and fewer surprises.</p><p><i>Cannot attend the broadcast on March 26? Please register anyway to automatically receive the link to the recording.</i></p><blockquote><p><strong>Presenter: </strong>Pascal Bächli (Sr. Engineer, Field Applications • Analytical Instrruments Group, Thermo Fisher Scientific)</p><p>Pascal Bächli is a Sr. Field Application Engineer at Thermo Fisher Scientific with over 12 years of laboratory experience in both GMP and non-GMP environments. He specializes in the administration, implementation, and support of laboratory data systems, including Thermo Scientific Chromeleon CDS and Waters Empower. He supports networked Chromeleon software installations, delivering consulting and user training, and troubleshooting for complex technical and workflow issues.</p><p>Pascal also holds a degree in Business IT, complementing his strong lab background and scientific expertise with deep knowledge of digital systems and business processes. This combination allows him to bridge the gap between the practical challenges scientists face and available technology to deliver efficient and compliant laboratory data solutions.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Chromeleon Admin Console II: System management and automation

Chromeleon Admin Console II: System management and automation

In this practical session, we’ll dive into more Administration Console capabilities that help you manage and standardize your setup at scale

Chromeleon Admin Console II: System management & automation

<p><strong>On March 31, Jennifer Knister, Commercial Product Manager - Process Solutions at Tosoh Bioscience, will discuss the importance of seamlessly scaling up downstream bioprocessing purification efforts from PD to GMP production using pre-packed chromatography columns. Participants will learn about the impact of multi-column chromatography (MCC) on column selection, along with the economics of pre-packed versus self-packed columns in MCC processes at production scale.</strong></p><p>This webinar will introduce SkillPak™ MAX pre-packed columns and present performance data using TOYOPEARL® Super A, a novel Protein A resin.</p><p><strong>After the webinar, ask your questions during the live Q&amp;A session.&nbsp;</strong></p><blockquote><p><strong>Presenter: </strong>Jennifer Knister (Commercial Product Manager - Process Solutions at Tosoh Bioscience)</p></blockquote>

Tosoh Bioscience: Tosoh Tuesday - Scaling Up from Process Development to GMP Production with Pre-packed Columns

Tosoh Tuesday - Scaling Up from Process Development to GMP Production with Pre-packed Columns

This webinar will introduce SkillPak™ MAX pre-packed columns and present performance data using TOYOPEARL® Super A, a novel Protein A resin.

From Process Development to GMP Production

<h5><strong>Key Learning Points</strong></h5><ul><li>Discover how to properly use and maintain your column to maximize its performance and lifespan.</li><li>Understand the physicochemical interactions that take place in chromatography.&nbsp;</li><li>Learn how to select columns and method conditions step-by-step.</li></ul><h5><strong>Who Should Attend</strong></h5><ul><li>Any liquid chromatography users who are looking for tips and best practices on developing proper methods and preventing troubleshooting.<strong>&nbsp;</strong></li></ul><blockquote><p><strong>Presenter: </strong>Koen Askamp (Senior Technical Specialist, Phenomenex)</p><p>Koen Askamp is a Senior Technical Specialist in Phenomenex. He was awarded his BSc and MSc degrees in Molecular Life Sciences at Wageningen University in the Netherlands and joined Phenomenex in 2020 as Technical Sales Consultant. Koen became part of Phenomenex’s global technical support team in 2022 and assists laboratories in training chromatographers and developing analytical methods.</p></blockquote>

Phenomenex: Recipe for Separation: Crafting Your Perfect HPLC Method

Recipe for Separation: Crafting Your Perfect HPLC Method

Discover how to properly use and maintain your column to maximize its performance and lifespan.