Modernizing Routine HPLC Methods: Aligning Productivity, Compliance, and Sustainability

<h3>How to Easily Adapt Your Existing HPLC Methods</h3>Developing and optimizing an HPLC method is essential for achieving reliable separations, accurate quantification, and reproducible analytical results. As chromatography technology advances, one of the most common optimization strategies is transferring established methods to modern columns with smaller dimensions.Although the benefits are clear, many analysts hesitate when it comes to modifying a validated method.<blockquote>Questions quickly arise: How should the flow rate be adjusted? What injection volume is appropriate? Does the gradient need to be recalculated? Will the retention times change?</blockquote>Even with the necessary equations available, performing all these calculations manually can be time-consuming and prone to errors. Instead of simplifying the workflow, method transfer often becomes an unnecessary obstacle.Fortunately, there is an easier solution.<h4>Simplify Your HPLC Method Transfer</h4>Moving an existing method to a different column does not have to be complicated.The KNAUER HPLC Method Converter is designed to automate the calculations required for method transfer. Simply enter the parameters of your current method, including the original column dimensions, and specify the dimensions of the new column—length, internal diameter, and particle size. The software instantly calculates the recommended flow rate and injection volume for the new conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_7f7c19f7a3.webp" alt="KNAUER: HPLC Method Converter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_7f7c19f7a3.webp 210w," sizes="100vw" width="1022" height="759">The next step is equally straightforward. Copy the original gradient profile into the converter, and the software automatically generates the corresponding gradient program for the new column.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_2_8ba46e78bc.webp" alt="KNAUER: HPLC Method Converter - Gradients" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_2_8ba46e78bc.webp 195w," sizes="100vw" width="1440" height="1150">For even greater confidence, the converter also allows you to enter chromatographic peak information. Based on these data, it predicts the expected retention times under the new conditions. While experimental verification on the HPLC system is still recommended, these predictions provide an excellent starting point for method optimization.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_3_34f16bb3bf.webp" alt="KNAUER: HPLC Method Converter - Chromatograms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_3_34f16bb3bf.webp 196w," sizes="100vw" width="1024" height="814">The converter was developed with one primary objective: making HPLC method adaptation faster, easier, and more reliable while reducing the effort required from the user. Its intuitive interface, modern design, and automatic calculations help save valuable laboratory time—and it is available free of charge.<h4>Modernize Your Methods and Improve Laboratory Efficiency</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_4_31e7188db4.webp" alt="KNAUER: Modernize Your Methods and Improve Laboratory Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_4_31e7188db4.webp 245w," sizes="100vw" width="720" height="449"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_5_815a7bf889.webp" alt="KNAUER: HPLC Method Converter - Results" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_5_815a7bf889.webp 194w," sizes="100vw" width="1440" height="1157">One of the major advantages of the KNAUER HPLC Method Converter is its compliance with USP General Chapter &lt;621&gt;.For pharmaceutical laboratories, chromatographic methods must comply with pharmacopeial requirements that ensure product quality and patient safety. Historically, many USP methods were developed using larger HPLC columns that no longer reflect the capabilities of modern chromatography systems.Current USP, European Pharmacopoeia (Ph. Eur.), and Japanese Pharmacopoeia (JP) guidelines now permit specific adjustments to column dimensions and particle size for both isocratic and gradient methods. These changes are allowed provided that defined relationships between chromatographic parameters are maintained.The KNAUER Method Converter automatically checks whether the selected column dimensions remain within the permitted USP limits. If the proposed changes comply with the guidelines, the software confirms that the method can be transferred while remaining pharmacopeia compliant. This removes uncertainty from the method conversion process and helps laboratories modernize their analytical workflows with confidence.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_6_c9d1e28c54.webp" alt="Unsplash: Illustrative picture" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_6_c9d1e28c54.webp 245w," sizes="100vw" width="720" height="449"><h4>Increase Productivity While Reducing Solvent Consumption</h4>Updating HPLC methods is not only about shortening analysis times—it also supports more sustainable laboratory practices.Using shorter columns together with optimized flow rates significantly reduces solvent consumption, lowering operating costs and decreasing the amount of hazardous chemical waste generated during routine analyses. Faster separations also improve instrument throughput, enabling more samples to be processed within the same amount of time.Modernizing chromatographic methods therefore delivers a dual benefit: greater laboratory productivity and a smaller environmental footprint. With only a few simple adjustments, laboratories can improve efficiency while supporting more sustainable analytical workflows.

HPLC method conversion free and easy

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 6th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by Agilent Technologies and Metrohm and posters by Shimadzu/ASMS, Thermo Fisher Scientific/ASMS and Waters Corporation/ASMS!<h3>1. Agilent Technologies: Determination of 41 Per- and Polyfluoroalkyl Substances (PFAS) in Cosmetics</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_captiva_emr_pfas_food_ii_cartridge_5994_9111en_agilent_be91861646.pdf">Full PDF for download</a></li></ul>Per- and polyfluoroalkyl substances (PFAS) are synthetic compounds composed of highly stable carbon–fluorine (C–F) bonds, giving them exceptional thermal and chemical stability, leading to poor environmental degradability. PFAS have been used in cosmetics to provide water resistance, oil repellency, or application smoothness. The unintentionally presented PFAS residues in raw materials may remain in finished cosmetic products, and can be absorbed through the skin. Lip products and spray type products can also lead to inhalation or oral exposure to residues.1,2&nbsp;Agilent Captiva EMR PFAS Food cartridges are designed to provide streamlined and comprehensive matrix removal after QuEChERS extraction for complex sample matrices, demonstrating effective matrix cleanup efficiency and excellent performance for quantitative PFAS analysis.3,4 In this study, we performed QuEChERS extraction followed by cleanup with Captiva EMR PFAS Food II cartridges for PFAS analysis in five types of cosmetics.<h4>Experimental</h4><h5>Equipment and materials&nbsp;</h5>The study was performed using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III">Agilent 1290 Infinity III LC</a> system consisting of an Agilent 1290 Infinity III high-speed pump (G7120A), an Agilent 1290 Infinity III multisampler (G7167B), and an Agilent 1290 Infinity III multicolumn thermostat (G7116B). The LC system was coupled to an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6495D">Agilent 6495D triple quadrupole LC/MS system</a> (G6495D) equipped with an Agilent Jet Stream Electrospray ion source. Agilent MassHunter Workstation software was used for data acquisition and analysis.&nbsp;The 1290 Infinity III LC system was modified using an Agilent InfinityLab PFAS Analysis HPLC conversion kit (part number 5004-0006), including an Agilent InfinityLab PFC delay column, 4.6 × 30 mm (part number 5062-8100). Chromatographic separation was performed using an Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=ZORBAX+RRHD+Eclipse+Plus">ZORBAX RRHD Eclipse Plus</a> C18, 95 Å, 2.1 × 100 mm, 1.8 µm (part number 959758-902).<h4>Results and discussion&nbsp;</h4><h5>Chromatography and calibration curve linearity&nbsp;</h5>Figure 2 shows the overlaid chromatograms for 41 PFAS calibration standards, including 0.05, 0.1, 0.2, 0.5, 1.0, 2.0, 5.0, and 10.0 ng/mL in ACN. The table on the right shows the peaks identification and corresponding retention time. Across 41 PFAS compounds, R² values ranged from 0.9940 to 0.9998, indicating excellent linearity for all analytes' calibration curves across the calibration range of 0.05 to 10.0 ng/mL.<h5>PFAS detection in real cosmetic samples&nbsp;</h5>Of the 15 cosmetic products assessed, one eyeshadow sample was detected with a trace level of positive PFAS analytes, including PFHeA, PFHpA, PFNA, and PFOA, at about 0.1 ng/g in the sample. Figure 5 shows the method blanks and positive detection of PFHpA and PFNA in this eyeshadow&nbsp;product.<h4>Conclusion&nbsp;</h4>A simplified, rapid, and reliable method using QuEChERS extraction followed by EMR mixed-mode passthrough cleanup using the Agilent Captiva EMR PFAS Food II cartridge and LC/MS/MS detection was developed for 41 PFAS analysis in cosmetics.<h3>2. Metrohm: brightRC – Advanced brightener analysis with a response curve</h3>Next-level brightener determination in nonlinear systems<ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_v_242_download_83376b2923.pdf">Full PDF for download</a></li></ul><h4>WHAT IS BRIGHTRC?&nbsp;</h4>brightRC is a response curve calibration approach for the determination of brightener-type additives by CVS or CPVS (Cyclic Pulse Voltammetric Stripping), hence the name «brightRC». This technique is suitable to measure brightener content in copper baths for plating. The method is based on signal normalization using the electrolyte value, which is the response for the virgin makeup solution (VMS) saturated with suppressor, and brightener defined as Q0 in the viva software. All recorded signals are divided by Q0 and the normalized values are plotted against concentration to generate an external calibration curve. This calibration can then be used for routine sample determination without repeated standard additions.&nbsp;The sample is simply measured using the same method as the calibration, signals are normalized and evaluated using the mathematically fitted calibration curve. By decoupling calibration from sample measurement, brightRC removes the need for repeated standard additions with each sample measurement, enabling faster and more efficient routine analysis.&nbsp;The brightRC calibration approach supports flexible regression models including quadratic and nonlinear regression. This allows reliable quantification of additive systems that do not exhibit a wide linear working range or exhibit nonlinear response characteristics, thereby expanding the range of applicable brightener chemistries.&nbsp;One important detail that further differentiates brightRC from the MLAT approach relates to normalization of the signal for calibration and sample analysis. This normalization approach improves measurement robustness as variations in absolute signal intensity naturally occurring over the working day are mathematically compensated.<h4>HOW DOES BRIGHTRC WORK IN PRACTICE?&nbsp;</h4>The brightRC workflow separates calibration and sample analysis. It can be implemented in different setups (from manual to fully automated) depending on the additive system and concentration range. After recording the electrolyte value, calibration standards are measured to establish the calibration curve, otherwise known as the response curve. Once the calibration is stored, samples are determined with the same CVS or CPVS method, without further standard additions. The sample concentration is automatically calculated from the normalized signal using the calibration curve.&nbsp;For sample measurement, viva 4.0 provides two practical approaches:&nbsp;<ol><li>«brightRC with solution exchange»&nbsp;</li><li>«brightRC with sample dilution» Both variants follow the same calibration principle and differ only in the sample handling strategy.</li></ol><h4>WHEN TO USE BRIGHTRC?&nbsp;</h4>brightRC is ideal for routine brightener control in stable plating baths where high sample throughput is required. It is particularly suitable when matrix conditions are consistent and electrode fouling is limited. The approach is especially advantageous for additive systems exhibiting nonlinear signal behavior. If strong matrix variations, significant breakdown products, or severe electrode fouling are expected and a linear correlation between signal and analyte concentration is guaranteed, standard addition techniques such as MLAT are preferred because they may provide additional robustness.<h3>3. Shimadzu / ASMS: PFAS Analysis of Leachate: Evaluating Key Sample Preparation and LC–MS/MS Parameters for Improved Performance</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/WP_255_PFAS_Analysis_of_Leachate_Evaluating_Key_Sample_Preparation_and_LC_MSMS_Parameters_for_Improved_Performance_1114ee264c.pdf">Full PDF for download</a></li></ul>Landfill leachate is a highly complex and variable matrix that poses significant challenges for the analysis of per- and polyfluoroalkyl substances (PFAS). Although U.S. Environmental Protection Agency Method 1633A provides a standardized framework for PFAS quantitation,1 analytical performance remains strongly influenced by sample preparation, chromatographic separation, and mass spectrometric conditions. Landfill leachate samples were extracted using WAX SPE following EPA 1633A and analyzed by LC–MS/MS using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8060">Shimadzu LCMS-8060</a> under negative ESI conditions. This study evaluates the key factors governing LC–MS/MS sensitivity, signal response, and reproducibility in landfill leachate, including a systematic assessment of SPE, chromatographic behavior, and ionsource conditions across PFAS classes (Figure 1).<h4>Results&nbsp;</h4>Chromatographic performance was evaluated across four C18 columns (A–D) using peak area, height, asymmetry, and HETP. Two columns produced a higher signal and improved peak shape, while the remaining columns showed greater efficiency. Under repeated batches followed by storage in acetonitrile, the Shimadzu GIST column produced a consistent signal (≤15% change), whereas another column showed 20–50% signal loss, particularly for long-chain PFAS and precursors, indicating greater susceptibility to matrix accumulation (Figure 2).<h4>Conclusion&nbsp;</h4><ul><li>PFAS analytical performance was dependent on compoundclass-dependent extraction, chromatography, and ionization behavior.&nbsp;</li><li>GIST demonstrated lower signal variability under repeated matrix exposure, with lower signal degradation than alternative C18 phases.</li><li>Neutral PFAS showed poor retention on WAX sorbents, requiring optimization beyond standard EPA 1633A extraction conditions.&nbsp;</li><li>PFCA response was more influenced by gas flow conditions, whereas PFSA response was more sensitive to source temperature conditions.</li></ul><h3>4. Thermo Fisher Scientific / ASMS: Ultra-high throughput proteomics at &gt;500 samples/day using advanced <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Vanquish Neo UHPLC</a> methods with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959">Orbitrap Astral Zoom MS</a></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004708_lsms_ultra_high_throughput_vanquish_astral_proteomics_po004708_asms2026_na_en_f6499c3eec.pdf">Full PDF for download</a></li></ul>High-throughput mass spectrometry is increasingly critical for large-cohort proteomics, population-scale studies, and screening applications. While established LC–MS platforms routinely support several hundred samples per day, further increases in throughput are often limited by chromatographic stability, robustness, and reproducibility under ultra-short gradient conditions, as well as the acquisition speed of the mass spectrometer. Here, we describe an advanced ultra-high-throughput LC–MS methodology developed on the Thermo Scientific Vanquish Neo UHPLC system and Thermo Scientific Orbitrap Astral Zoom mass spectrometer. The goal of this work was to extend the practical throughput limits of LC–MS while achieving deep proteome coverage and maintaining run-to-run reproducibility.<h4>Materials and methods</h4><h5>LC-MS analysis&nbsp;</h5>Samples were separated on a Thermo Scientific 150 µm × 70 mm C18 column (prototype, not commercially available yet) operated at 4 µL/min using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Vanquish Neo UHPLC system</a> in NanoCap Trap&amp;Elute configuration at room temperature. Prototype methods allowing for reduced LC duty cycles (approx. 1 min of sample pickup/loading and integrated trap-column offline wash) were applied, the standard gradients were adjusted to 1 – 3 min. The Thermo Scientific PepMap Neo 5 µm C18 300 µm × 5 mm cartridge was used as trap column and the separation column connected with a 15 µm ID capillary Thermo Scientific EASY-Spray emitter (ES994) to an EASY-Spray source. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959">Orbitrap Astral Zoom MS</a> (ICSW AST 2.2) was operated in a dataindependent acquisition (DIA) mode, MS method parameters are given in Table 1. Comparative data for the chemoproteomics samples at lower throughputs (60-300 SPD) were acquired using an EASY-Spray 150 µm × 150 mm C18 column (ES906), as described before.&nbsp;<h5>Data analysis&nbsp;</h5>The raw data files were processed with Spectronaut® 20.5 software (Biognosys AG), <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782">Thermo Scientific Proteome Discoverer </a>3.3 SP1 with CHIMERYS 5.0 or DIA-NN Enterprise 2.3.2 (Aptila Biotech GmbH) nodes using default settings as specified in the individual figures. The data was searched against the Homo sapiens SwissProt database (TaxID=9606: 42,252 sequences) and contaminants FASTA file. Peptide numbers correspond to unique stripped peptides<h4>Conclusions&nbsp;</h4><ul><li>The advanced Vanquish Neo UHPLC methodology in combination with the Orbitrap Astral Zoom MS delivered highly reproducible, deep and precise proteome profiling across ultra-high-throughput regimes (600 SPD) exceeding current field benchmarks.&nbsp;</li><li>The workflow enables rapid chemoproteomic screening, as demonstrated with activity-based protein profiling (ABPP) samples, reliably identifying enriched putative target proteins of an antibiotic small molecule probe.</li></ul><h3>5. Waters Corporation / ASMS: Assessing Relative Response of Four European-Regulated PFAS in Human Serum Using Cyclic Ion Mobility MS</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009410_c98e7c87ec.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster presents an LC-Cyclic-IMS-MS approach for assessing four European-regulated PFAS—PFOS, PFOA, PFNA, and PFHxS—in human serum. The study focuses on environmental exposure monitoring in Ghanaian firefighters and e-waste handlers, using ion mobility mass spectrometry to add separation power and improve identification confidence in complex biological matrices. The work is motivated by increasing regulatory concern around PFAS toxicity, bioaccumulation, and the need for reliable exposure assessment in human biofluids.The experimental workflow combined reversed-phase LC with a quadrupole-cyclic ion mobility-time-of-flight MS system (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/202">SELECT SERIES Cyclic IMS</a>). Human serum samples were prepared using SPE µElution plates with a polymeric reversed-phase/weak anion exchange sorbent. The LC system was modified with PFAS-free components and an isolator column to reduce background contamination, which is especially important for trace-level PFAS analysis. Identification was supported by retention time, accurate mass, isotopically labelled internal standards, and collision cross section values.The results showed detectable levels of the four regulated PFAS in both occupational groups, with a combined detection rate of 83% across human serum samples and NIST reference standards. Firefighter samples generally showed higher concentration ranges than e-waste handler samples, especially for PFOS, PFOA, PFNA, and PFHxS. For example, PFNA reached up to 5.713 ng/mL in firefighter serum, compared with up to 1.513 ng/mL in e-waste handler serum.Overall, the poster concludes that LC-Cyclic-IMS-MS provides a highly specific strategy for PFAS analysis in complex biological samples. Ion mobility and CCS values improve confidence in trace-level identification and help distinguish target PFAS from co-eluting matrix background. The authors suggest that this approach can support both targeted PFAS monitoring and broader non-targeted screening of known and emerging PFAS, while also helping future studies connect exposure data with environmental fate and potential toxicological effects.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 28, 2026

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<h4>European Free Educational Webinar Series</h4>
Let&#39;s go back to the basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.
<h4>EPISODE 6</h4>
The importance of Compliance
What will you learn?
<ul>
<li>Data Integrity Matters - A short Overview</li>
<li>What is important in your Lab Environment</li>
</ul>
<blockquote>
Prezenter: Arjan Timmerman (Pharma Market Development Manager EMEA, Waters Corporation)
</blockquote>
<blockquote>
Arjan Timmerman is a market development manager at Waters working in EMEA. Prior to joining Waters in 2014 he worked for 17 years as an Analytical Scientist in the Small Molecule Pharmaceutical industry in various Chemical and Pharmaceutical late-stage development labs.
</blockquote>
<blockquote>
Since 2023 he became QA/QC market development manager for EMEA working in a dedicated team and focuses on Pharmaceutical workflows, AQbD, Impurities, LC Chromatography and Compliance.
</blockquote>
<h4>What&#39;s next?</h4>
Full Webinar Series Episodes List is available <a href="https://event.on24.com/wcc/r/4551012/F69479929A905500D8B21508527807E5/5385652">here</a>
Back to Basics - Sample Preparation: Theory and Overview
<ul>
<li>Tuesday, May 07, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Sample Preparation: Tips &amp; Tricks to make it simple
<ul>
<li>Wednesday, June 05, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Theory of Liquid Chromatography
<ul>
<li>Wednesday, July 03, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Basics of Liquid Chromatography
<ul>
<li>Tuesday, September 10, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Analytical Method Development
<ul>
<li>Wednesday, October 02, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - The importance of Compliance
<ul>
<li>Tuesday, October 22, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Introduction to Mass Spectrometry
<ul>
<li>Wednesday, November 13, 2024, 11:00 AM CET</li>
</ul>
Back to Basics - Troubleshooting your LC and MS Systems
<ul>
<li>Tuesday, December 10, 2024, 11:00 AM CET</li>
</ul>
Not sure to be able to attend live sessions?
Register today and watch the webinars on-demand

Waters Corporation: Back to Bacisc Free Educational Webinar Serries

The importance of Compliance

Why data integrity matters and what is important in your lab environment.

<h4>Virtual event: Pharma conclave series 2024</h4>SCIEX offers the platform for the experts to take the stage and drive technological progressWelcome to Pharma conclave series 2024 – where our customers’ expertise meets innovation, transforming your knowledge and empowering you to revolutionize your work processes. Join us in discovering techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.From the research of new markers to the drug development moving into non and GLP bioanalysis. Confidently plan your next lab expansion with the knowledge gained from industry leaders and pioneers.<ul><li>May 16 Event 1: Pharma omics</li><li>September 17 Event 2: Impurities</li><li>October 23-24 Event 3: Pharma</li></ul>10:00 a.m.–12:00 p.m. CET<h3>Event 3: Pharma</h3>The pharma connectionFrom discovery to QA/QC. All the process to bring a new treatment to the patients is following a path to connect the different stages until the magic happens.Join to see how the scientists are generating the needed tricks to connect the initial though with the final formulation.<h3>Day 1</h3><h5>10:00 - 10:30 - Metabolomics screening kits for use in clinical trials</h5><ul><li>Heike Wiese, Nuvisan</li></ul><h5>10:30 – 11:00&nbsp;- Sphingolipid biomarkers in neurodegenerative drug development</h5><ul><li>Jose Carlos Gonzalez, AMSbiopharma</li></ul><h5>11:00 – 11:30 - Parallel setup of a series of micro LC methods, coupled with the low-resolution mass spec (API7500) for &nbsp;TG2 inhibitors</h5><ul><li>Davide Massari and Chiara Olmi, ERBC Group</li></ul><h5>11:30 – 12:00&nbsp;- PFAS analysis and mass spectrometry: Beyond environmental, food and packaging. What about cosmetic and pharmaceutical products?</h5><ul><li>Emiliano De Dominicis, Merieux</li></ul><blockquote>Presenter: Bruno Manadas, PhD</blockquote><blockquote>Dr Manadas is a prominent Cell Biology researcher at CNC-UC since 2017. With expertise in biochemistry, psychology, and biotechnology, he's authored 130+ publications and led multiple projects. As head of CNC's Mass Spectrometry node and Associate Editor for Frontiers, he's contributed significantly to neuroscience and psychiatry. Bruno's research focuses on cell biology mechanisms, translational biomarker research, and MS-based approaches for studying protein oxidation. He played a pivotal role in establishing the hospital biobank and teaches at the Portuguese Catholic University.</blockquote><blockquote>Presenter: Dorte Bekker-Jensen</blockquote><blockquote>Dorte is heading the Applications Development team in Proteomics Research at Evosep. She has long-term experience within proteomics and has focused on technology development by developing simple and automated workflows for high-throughput applications.</blockquote><blockquote>Presenter: Stefan Ledinger</blockquote><blockquote>Stefan has 20 years of experience in the life sciences and diagnostic industries. For the last dozen years, he has been serving various functions within biocrates life sciences ag. Most recently, he has been responsible for the market development for metabolomics solutions in the pharmaceutical and biotech industries. He currently serves as Global Head of Sales. Stefan holds degrees in business administration and bioengineering, combining commercial know-how with a passion for science.</blockquote><blockquote>Presenter: Markus Langsdorf</blockquote><blockquote>Markus has 14 years of experience in life sciences industries. As he joined biocrates in 2010, he worked for 11 years as application support specialist for biocrates’ metabolomics profiling solutions. 3 years ago he changed his position withing the company to his new function as product manager. Markus studied chemistry and graduated with a PhD in Analytical Chemistry.</blockquote>

Pharma conclave series 2024: Pharma (Day1)

From discovery to QA/QC. The process to bring a new treatment to the patients.

This webinar is about single quad mass detectors. We will look at the development of single quads together and highlight the possibilities offered by today's single quads and how they support the user.The aim of the webinar is to show users how they can easily add mass-selective confirmation to their HPLC-UV methods. In other words: achieve greater security without more complexity.<blockquote>Presenter: Shaun Pritchard (Product Specialist Liquid Phase Separations, Agilent Technologies)Shaun Pritchard is a Product Specialist for separation products at Agilent Technologies covering the UK and Ireland. He started his career in 1989 working at a large Pharmaceutical company holding a number of roles, this is where he became passionate about HPLC finally in 1998 he took the leap to leave the lab and move to the commercial side of things and has had a number of roles within the industry until 2017 he joined Agilent.</blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Single Quad mass detection II

We will look at the development of single quads together and highlight the possibilities offered by today's single quads and how they support the user.

This webinar will guide you through optimizing your Q-TOF for superior data quality by selecting the most suitable tune mode for your samples.You will learn about the different optimisation workflows available within MassHunter Acquisition, including how to optimise Multiple Reaction Monitoring (MRM) methods for new compounds. We will also review key tools for creating and editing worklists, and discuss the benefits of Intelligent Reflex tools, demonstrating how they can be effectively implemented in your workflows.This webinar is designed to enhance your understanding and application of MassHunter Acquisition, ensuring you can achieve optimal performance in your analytical processes.<blockquote>Presenter: Day Powell (Field Application Specialist, Agilent Technologies)Day has worked for Agilent as an Environmental Application Specialist for over a year, before this he worked for United Utilities (UU) as a Technical Specialist responsible for developing new methodologies for both LC/MS and GC/MS applications. Day has worked with PFAS for several years and helped UU to UKAS accredit a novel method for 47 PFAS in drinking water using direct injection to address the latest DWI regulations. Presenter: Jonathan Spencer (Application Specialist, Agilent Technologies)Jonathan studied BSc Natural Sciences at the University of Nottingham and later went on to Sheffield Hallam University to study a MSc in Forensic Science.Following graduation, he spent 2 years as a Confirmatory Analyst in an anti-doping and forensic toxicology lab, before joining Agilent as an LC/MS Applications Scientist. At Agilent, he focuses on small molecule applications using LC/TQ and LC/Q-TOF in fields of like food, forensic and environmental analysis.</blockquote>

Agilent Technologies: MassHunter Masterclass Months

MassHunter Acquisition for LC/TQ and MRM Method Optimisation

This session will provide a comprehensive overview of the various aspects of MassHunter Acquisition for Liquid Chromatography/Triple Quadrupole (LC/TQ) systems.

Automated lab workflows often require many interactions with pre-existing in-house and commercial software systems. This webinar uses the real example of building a purification factory, to give some insights on how this can be done with Mestrelab tools in conjunction with in-house and commercial software.<div data-aos-delay="0" data-aos-duration="1000"><div>Join us for an insightful webinar where we’ll demonstrate how to integrate Mestrelab’s tools into a complex IT ecosystem to automate lab workflows. Using a real-world example, we'll show you how to build a purification factory that seamlessly connects in-house and commercial software systems.<p data-ccp-props="{&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559683&quot;:0,&quot;335559685&quot;:0,&quot;335559731&quot;:0,&quot;335559737&quot;:0,&quot;335562764&quot;:2,&quot;335562765&quot;:1,&quot;335562766&quot;:4,&quot;335562767&quot;:10,&quot;335562768&quot;:4,&quot;335562769&quot;:0}">Who Should Attend?​<ul style="font-weight:400;"><li data-charcodes="8226" data-font="Arial,Sans-Serif" data-buautonum="8" data-aria-posinset="1" data-aria-level="1">Lab Managers and Scientists looking to automate and enhance lab workflows​</li><li data-charcodes="8226" data-font="Arial,Sans-Serif" data-buautonum="8" data-aria-posinset="2" data-aria-level="1">IT and Automation Specialists in life sciences or chemical industries seeking integration solutions​</li><li data-charcodes="8226" data-font="Arial,Sans-Serif" data-buautonum="8" data-aria-posinset="3" data-aria-level="1">Anyone interested in embedding Mestrelab tools into a powerful, scalable IT infrastructure</li></ul><blockquote>Presenter: John Hollerton (Senior Scientific Director, Mestrelab Research)John has over 40 years of experience in the scientific and informatics fields at GSK and its predecessor companies. He has managed various analytical functions, including NMR, X-ray crystallography, mass spectrometry, physical chemistry, and chromatography, and has expertise in chemical structure representation and registration. In informatics, he led projects to create a global scientific data management system and wrote software for instrument control, data transformation, and analysis. His team developed software to automate LCMS purification factories in the US and UK.&nbsp;</blockquote></div></div>

Mestrelab: Building a Purification Factory: Embedding Mestrelab Tools into a Rich IT Infrastructure

Building a Purification Factory: Embedding Mestrelab Tools into a Rich IT Infrastructure

We’ll demonstrate how to integrate Mestrelab’s tools into a complex IT ecosystem to automate lab workflows.

Embedding Mestrelab Tools into a Rich IT Infrastructure

<div data-lineheight="1.5">Food analysis is crucial for consumer safety, authenticity, and pollutant detection. Benchmark analytical methods use complex protocols and organic solvents, and more sustainable alternatives are to be sought out. A recent trend in analytical chemistry leverages raw data from experiments (e.g., chromatographic profiles, spectra) as sample fingerprints, analyzed with multivariate tools to predict characteristics (e.g., origin, composition) and identify adulterations. These approaches reduce analyses, lab work, and resource use, offering greener, more cost-effective alternatives.The webinar will show how to combine the two aims, sustainability and data maximization, using an aqueous colloidal system, developing AF4-DAD-MALS-dRI-FLD platforms to separate and characterize components from food matrices:</div><ul><li data-lineheight="1.5">Red wine profiling and in-depth analysis of output data can highlight particulate complexity, explain sensory feedback, and lead to cultivar grouping.</li><li data-lineheight="1.5">Milk analysis and multivariate elaboration of fractograms enabled single-run discrimination of factors like thermal treatment, fat content, and even the manufacturing plant’s impact on colloidal content.</li></ul>These AF4 method are fast, uses minimal sample preparation, avoid chemical treatments, offering a greener, faster and possibly cheaper alternative. The FFF approach can enhance food quality control and fraud detection processes and is adaptable to other colloidal food samples. Additionally, it underscores the need to increase multivariate analysis in data from FFF platforms.<h4>Key Learning Objectives:</h4><ul><li data-lineheight="1.5">Tackle native sample handling</li><li data-lineheight="1.5">Understanding data extraction from multidetection profiling</li><li data-lineheight="1.5">Rationalizing multivariate analysis of FFF data</li></ul><h4>Who Should Attend:</h4><ul><li data-lineheight="1.5">Laboratory managers</li><li data-lineheight="1.5">Chromatographers</li><li data-lineheight="1.5">Scientist in the Food &amp; beverage industry</li></ul><blockquote>Presenter: Valentina Marassi, Ph.D. (University of Bologna, byFlow srl)Dr. Marassi is Tenure-Track Researcher and Assistant Professor in Analytical Chemistry at the University of Bologna. She graduated with a B.S. in chemistry, a Master’s in Organic Chemistry and completed a PhD in Analytical Chemistry in the same Institution. Her core research involves separation science and profiling-multidetection applied to nano-bio samples, and developing FFF-chemometrics strategies to model and understand colloidal profiles.Presenter: Prof. Andrea Zattoni (University of Bologna, byFlow srl)Andrea Zattoni joined the University of Bologna in 2006 and is currently an Associate Professor. He holds an M.S. in Chemistry and a Ph.D. in Chemical Sciences from the University of Bologna with a research focus on separation science. His main research interests include the fundamental and instrumental development of field-flow fractionation and its combination with highly sensitive and selective detection techniques.Presenter: Udayabagya (Bud) Halim (Waters | Wyatt Technology)Bud Halim is a senior marketing analyst at Wyatt Technology. He holds a B.S. and Ph.D. in physical chemistry from the UCLA. Bud started his career as an adjunct professor where he taught courses such as Biochem, Analytical, and Physical Chemistr. Prior to joining Wyatt Technology, Bud worked in the scientific instrumentation field as an applications scientist for three years. His experience spans the characterization of batteries, semiconductors, and the stability of biologics, LNPs, and viral vectors.</blockquote>

Waters Corporation: Colloidal Fingerprints of Food and Beverages with multidetector AF4-MALS

Colloidal Fingerprints of Food and Beverages with multidetector AF4-MALS

How to combine sustainability and data maximization, using an aqueous colloidal system, developing AF4-DAD-MALS-dRI-FLD platforms.

Fingerprints of Food and Beverages with AF4-MALS

<div><div>Therapeutic synthetic oligonucleotides represent unique analytical challenges in late-stage development. To meet the stringent regulatory requirements, highly specific and sensitive methods such as liquid chromatography-mass spectrometry (LC/MS) must be used. However, neither chromatography nor mass spectrometry data systems have the capabilities, both in function and in data integrity, for the typical QC analyst end user to run a highly complex method such as purity, assay, and impurity profiling for therapeutic oligos such as anti-sense oligonucleotides.In this presentation, we present a paradigm shift in software with Oligo Analysis Accelerator for OpenLab CDS. This application streamlines the analytical workflow by using a fit-for-purpose user interface while leveraging existing data integrity and compliance architecture within OpenLab CDS.<h4>By attending this webinar, you will:</h4><ul><li>Understand the fundamentals of ion-pair reversed-phase LC/MS for oligonucleotides</li><li>Discover how a single quadrupole detector is used for impurity profiling of anti-sense oligonucleotides</li><li>Learn how fit-for-purpose software for LC/MS analysis in QC lot release can save time and enhance quality</li></ul><h5>Who should attend</h5><ul><li>Analytical chemists interested in cutting-edge software for large molecule late-stage development</li><li>Analytical research and development labs interested in developing robust methods for single-stranded oligo therapeutics</li><li>Process scientists who rely on in-process control purity results to optimize synthesis and purification for oligos</li><li>Quality assurance and quality control personnel supporting oligo testing labs</li></ul><blockquote>Presenter: Brian Rivera (Software Product Manager, Agilent Technologies)Brian Rivera is a software product manager at Agilent Technologies with 20 years of experience in HPLC analysis of large molecules.</blockquote></div></div>

Separation Sciences: Boost Efficiency in Oligonucleotide QC with Software Designed for the Job

Boost Efficiency in Oligonucleotide QC with Software Designed for the Job

In this presentation, we present a paradigm shift in software with Oligo Analysis Accelerator for OpenLab CDS.

Oligonucleotide QC with Software Designed for the Job

In this webinar, Dr Stuart Best, Deputy Director, Drug Metabolism and Pharmacokinetics (DMPK), Sygnature Ltd, will provide an overview of analytical approaches in Discovery Bioanalysis CNS measurement, to deliver more information from lower sample volume and align with the 3R principles.Drug discovery programs that target the Central Nervous System require bioanalytical measurement of test compounds and neurotransmitter concentrations to establish a pharmacokinetic-pharmacodynamic (PK-PD) relationship. Bioanalytical assessment of unbound compounds in blood, brain tissue, and cerebrospinal fluid describes its distribution for the systemic circulation into the CNS. The inherently high sensitivity and specificity of LC-Triple Quadrupole Mass Spectrometry enables low level detection in small sample volumes, allowing serial sampling from single animals, reducing animal use and inter-animal variability, supporting the 3Rs principles (replacement, reduction, refinement)The bioanalysis of neurotransmitters such acetylcholine and monoamines, is challenging due to low molecular weight, high polarity, instability, and low endogenous concentrations. At Sygnature, the in vivo Pharmacology team employ microdialysis to permit assessment of neurotransmitter changes in specific brain regions. The low endogenous concentrations in brain regions and collection through dialysis probe into artificial CSF lowers further quantitation limits for neurotransmitter detection and lead to the requirements for highly sensitive and specific analytical approaches.This presentation will describe the above approached to CNS measurement and how LC-Triple Quadrupole Mass Spectrometry enables Sygnature scientists to align to the 3R principles and maximize data output from small samples and relate test compound concentrations to neurotransmitter changes. Truly, achieving on the concept of ‘More from Less’ Stuart will also present further refinement models for assessing test compound impact on acetylcholine and enabling more physiologically relevant measurements.<h4>Key Learning Objectives:</h4><ul><li>Understand the challenges as CNS measurements of test compounds and neurotransmitter concentrations to establish PK-PD relationships</li><li>Explore the complexities of neurotransmitter bioanalysis in discovery DMPK due to low concentrations and instability</li><li>Learn the roles of LC-Triple Quadrupole Mass Spectrometry enabling sensitive detection in small samples and alignment to the 3Rs (replacement, reduction, refinement) principles</li></ul><div><blockquote>Presenter: Dr Stuart Best (Deputy Director, DMPK, Sygnature Ltd)Dr Best's expertise spans regulated and discovery bioanalysis and discovery DMPK, from supporting DMPK screening (solubility, log D, metabolic stability, permeability, plasma protein binding, etc) through in vivo assessment of compounds to more detailed profiling to support candidate nomination and human dose prediction, including the assessment of potential risk from drug-drug interactions.During his time in the industry Dr Best has expanded the utilisation of mass spectrometry (specifically triple quadrupole LC-MS/MS) to help solve drug discovery project challenges with a strong focus on maximizing data capture from in vivo samples, refining experimental designs to permit better PD endpoints and expanding LC-MS/MS support for biological screening assays, where a mass spectrometry end-point is required, for the measurement of more challenging endogenous analytes.</blockquote></div>

More from less: LC-MS/MS an Enabling Technology to Maximize Bioanalytical Data Collection from Small Sample Volumes

An overview of analytical approaches in Discovery Bioanalysis CNS measurement, to deliver more information from lower sample volume.

LC-MS/MS to Maximize Bioanalytical Data Collection

Biotherapeutics offer targeted therapies for treating a wide range of diseases with greater specificity and efficacy. However, unlike traditional small-molecule drugs, biotherapeutics are typically larger and more complex, requiring advanced techniques for their production, purification, and characterization.Therapeutic proteins, first introduced many years ago, have continually advanced in complexity. Since the introduction of monoclonal antibodies, we now have antibody drug conjugates, bispecific antibodies, fragments and fusion proteins, all of which are in various stages of development.&nbsp;To keep pace with biotherapeutics advances, analytical techniques must also advance.&nbsp;<h5>In this webinar, we will present chromatography findings on:</h5><ul><li>New workflows for intact and peptide mapping characterization of proteins</li><li>Advanced column chemistries for charge variant analysis with pH and salt gradients&nbsp;</li><li>Rapid desalting SEC column for high throughput analysis, ADC analysis with HIC and reversed phase chemistries</li><li>New digestion workflows coupled to a new peptide mapping column for sequence verification and post translation modification analysis</li></ul>This live webinar will be an excellent opportunity to boost your R&amp;D capabilities and strengthen your QC analytical methods for the characterization of biotherapeutics and gene therapies. Dr. Ken Cook will be presenting and answering your questions live.With over 20 years of innovation, Thermo Fisher Scientific is a leader in biomolecule separation and characterization, supported by a wealth of published research.Who should attend?&nbsp;<ul><li>Pharmaceutical and Biotech Researchers: Researchers involved in the development and characterization of biotherapeutics and gene therapies, including those working with therapeutic proteins.</li><li>Quality Control (QC) Analysts: Analysts responsible for ensuring the quality and consistency of biotherapeutic products, who will benefit from advanced methods in characterization and validation.</li><li>&nbsp;R&amp;D Managers: Managers who oversee research and development teams working with biotherapeutics, looking to enhance their workflows and methodologies.</li></ul><blockquote>Presenter: Dr. Ken Cook (Application Scientists Manager, Thermo Fisher Scientific)Dr. Ken Cook began his career in academia as a Biochemistry lecturer focused on protein chemistry and metabolism. With over 30 years of industry experience at Dionex, and later Thermo Fisher Scientific, his vast knowledge supporting and developing solutions within chromatography has established him as a bio-separations expert.Currently, Dr. Cook focuses on supporting and developing solutions for bio-pharmaceutical applications, particularly in the characterization of protein and oligonucleotide-based biotherapeutics. His work involves ongoing collaborations with both the biopharmaceutical industry and academic institutions.His expertise includes peptide mapping using LC/HRMS, charged variant analysis with novel MS coupling, glycan analysis, aggregate analysis, and on-line process monitoring. He also has a growing interest and notable success in gene therapy characterization.Given his immense experience, it is an honour to have Dr Cook share his knowledge.</blockquote>

Thermo Fisher Scientific: Innovations in Protein Characterization

Innovations in Protein Characterization

Boost your R&D capabilities and strengthen your QC analytical methods for the characterization of biotherapeutics and gene therapies.

Christian Rohrer, Director Sales and Support at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.&nbsp;Analytical scientists are faced with the task of finding the right column from an almost unmanageable range of products. On Tuesday, October 22, Andrea Krumm, Product Manager Analytical Columns at Tosoh Bioscience, will focus on columns that enable protein analysis under native conditions through size exclusion, hydrophobic interaction and ion exchange chromatography. We will highlight the different column characteristics (pore size, particle size, base matrices, column dimensions, ligands) and which questions will help you decide which columns to use.After the webinar, ask your questions during the live Q&amp;A session.<blockquote>Presenter: Andrea Krumm (Product Manager Analytical Columns at Tosoh Bioscience)</blockquote>

Tosoh Bioscience: How to select (U)HPLC columns for protein analysis?

How to select (U)HPLC columns for protein analysis?

Columns that enable protein analysis under native conditions through size exclusion, hydrophobic interaction and ion exchange chromatography