Modernizing Routine HPLC Methods: Aligning Productivity, Compliance, and Sustainability

<h3><strong>How to Easily Adapt Your Existing HPLC Methods</strong></h3><p>Developing and optimizing an HPLC method is essential for achieving reliable separations, accurate quantification, and reproducible analytical results. As chromatography technology advances, one of the most common optimization strategies is transferring established methods to modern columns with smaller dimensions.</p><p>Although the benefits are clear, many analysts hesitate when it comes to modifying a validated method.</p><blockquote><p>Questions quickly arise: <i><strong>How should the flow rate be adjusted? What injection volume is appropriate? Does the gradient need to be recalculated? Will the retention times change?</strong></i></p></blockquote><p>Even with the necessary equations available, performing all these calculations manually can be time-consuming and prone to errors. Instead of simplifying the workflow, method transfer often becomes an unnecessary obstacle.</p><p>Fortunately, there is an easier solution.</p><h4><strong>Simplify Your HPLC Method Transfer</strong></h4><p>Moving an existing method to a different column does not have to be complicated.</p><p>The <strong>KNAUER HPLC Method Converter</strong> is designed to automate the calculations required for method transfer. Simply enter the parameters of your current method, including the original column dimensions, and specify the dimensions of the new column—length, internal diameter, and particle size. The software instantly calculates the recommended flow rate and injection volume for the new conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_7f7c19f7a3.webp" alt="KNAUER: HPLC Method Converter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_7f7c19f7a3.webp 210w," sizes="100vw" width="1022" height="759"></p><p>The next step is equally straightforward. Copy the original <strong>gradient profile</strong> into the converter, and the <strong>software automatically generates the corresponding gradient program for the new column</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_2_8ba46e78bc.webp" alt="KNAUER: HPLC Method Converter - Gradients" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_2_8ba46e78bc.webp 195w," sizes="100vw" width="1440" height="1150"></p><p>For even greater confidence, the converter also allows you to <strong>enter chromatographic peak information</strong>. Based on these data, it <strong>predicts the expected retention times</strong> under the new conditions. While experimental verification on the HPLC system is still recommended, these predictions provide an excellent starting point for method optimization.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_3_34f16bb3bf.webp" alt="KNAUER: HPLC Method Converter - Chromatograms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_3_34f16bb3bf.webp 196w," sizes="100vw" width="1024" height="814"></p><p>The converter was developed with <strong>one primary objective:</strong> making <strong>HPLC method adaptation faster, easier, and more reliable </strong>while reducing the effort required from the user. Its intuitive interface, modern design, and automatic calculations help save valuable laboratory time—and it is available free of charge.</p><h4><strong>Modernize Your Methods and Improve Laboratory Efficiency</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_4_31e7188db4.webp" alt="KNAUER: Modernize Your Methods and Improve Laboratory Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_4_31e7188db4.webp 245w," sizes="100vw" width="720" height="449"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_5_815a7bf889.webp" alt="KNAUER: HPLC Method Converter - Results" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_5_815a7bf889.webp 194w," sizes="100vw" width="1440" height="1157"></p><p>One of the major advantages of the <strong>KNAUER HPLC Method Converter</strong> is its compliance with <strong>USP General Chapter &lt;621&gt;</strong>.</p><p>For <strong>pharmaceutical laboratories</strong>, chromatographic methods must comply with pharmacopeial requirements that ensure product quality and patient safety. Historically, many USP methods were developed using larger HPLC columns that no longer reflect the capabilities of modern chromatography systems.</p><p>Current USP, <strong>European Pharmacopoeia (Ph. Eur.)</strong>, and <strong>Japanese Pharmacopoeia (JP)</strong> guidelines now permit specific adjustments to column dimensions and particle size for both isocratic and gradient methods. These changes are allowed provided that defined relationships between chromatographic parameters are maintained.</p><p>The KNAUER Method Converter automatically checks whether the selected column dimensions remain within the permitted USP limits. If the proposed changes comply with the guidelines, the software confirms that the method can be transferred while remaining pharmacopeia compliant. This removes uncertainty from the method conversion process and helps laboratories modernize their analytical workflows with confidence.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_6_c9d1e28c54.webp" alt="Unsplash: Illustrative picture" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_6_c9d1e28c54.webp 245w," sizes="100vw" width="720" height="449"></p><h4><strong>Increase Productivity While Reducing Solvent Consumption</strong></h4><p>Updating HPLC methods is not only about shortening analysis times—it also supports more sustainable laboratory practices.</p><p>Using shorter columns together with optimized flow rates significantly reduces solvent consumption, lowering operating costs and decreasing the amount of hazardous chemical waste generated during routine analyses. Faster separations also improve instrument throughput, enabling more samples to be processed within the same amount of time.</p><p>Modernizing chromatographic methods therefore delivers a dual benefit: greater laboratory productivity and a smaller environmental footprint. With only a few simple adjustments, laboratories can improve efficiency while supporting more sustainable analytical workflows.</p>

HPLC method conversion free and easy

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 6th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you application notes by <strong>Agilent Technologies </strong>and<strong> Metrohm </strong>and posters by<strong> Shimadzu/ASMS, Thermo Fisher Scientific/ASMS </strong>and <strong>Waters Corporation/ASMS!</strong></p><h3><strong>1. Agilent Technologies: Determination of 41 Per- and Polyfluoroalkyl Substances (PFAS) in Cosmetics</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_captiva_emr_pfas_food_ii_cartridge_5994_9111en_agilent_be91861646.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Per- and polyfluoroalkyl substances (PFAS) are synthetic compounds composed of highly stable carbon–fluorine (C–F) bonds, giving them exceptional thermal and chemical stability, leading to poor environmental degradability. PFAS have been used in cosmetics to provide water resistance, oil repellency, or application smoothness. The unintentionally presented PFAS residues in raw materials may remain in finished cosmetic products, and can be absorbed through the skin. Lip products and spray type products can also lead to inhalation or oral exposure to residues.<sup>1,2</sup>&nbsp;</p><p>Agilent Captiva EMR PFAS Food cartridges are designed to provide streamlined and comprehensive matrix removal after QuEChERS extraction for complex sample matrices, demonstrating effective matrix cleanup efficiency and excellent performance for quantitative PFAS analysis.<sup>3,4</sup> In this study, we performed QuEChERS extraction followed by cleanup with Captiva EMR PFAS Food II cartridges for PFAS analysis in five types of cosmetics.</p><h4><strong>Experimental</strong></h4><h5><strong>Equipment and materials&nbsp;</strong></h5><p>The study was performed using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III"><strong>Agilent 1290 Infinity III LC</strong></a> system consisting of an Agilent 1290 Infinity III high-speed pump (G7120A), an Agilent 1290 Infinity III multisampler (G7167B), and an Agilent 1290 Infinity III multicolumn thermostat (G7116B). The LC system was coupled to an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6495D"><strong>Agilent 6495D triple quadrupole LC/MS system</strong></a> (G6495D) equipped with an Agilent Jet Stream Electrospray ion source. Agilent MassHunter Workstation software was used for data acquisition and analysis.&nbsp;</p><p>The 1290 Infinity III LC system was modified using an Agilent InfinityLab PFAS Analysis HPLC conversion kit (part number 5004-0006), including an Agilent InfinityLab PFC delay column, 4.6 × 30 mm (part number 5062-8100). Chromatographic separation was performed using an Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=ZORBAX+RRHD+Eclipse+Plus"><strong>ZORBAX RRHD Eclipse Plus</strong></a> C18, 95 Å, 2.1 × 100 mm, 1.8 µm (part number 959758-902).</p><h4><strong>Results and discussion&nbsp;</strong></h4><h5><strong>Chromatography and calibration curve linearity&nbsp;</strong></h5><p>Figure 2 shows the overlaid chromatograms for 41 PFAS calibration standards, including 0.05, 0.1, 0.2, 0.5, 1.0, 2.0, 5.0, and 10.0 ng/mL in ACN. The table on the right shows the peaks identification and corresponding retention time. Across 41 PFAS compounds, R² values ranged from 0.9940 to 0.9998, indicating excellent linearity for all analytes' calibration curves across the calibration range of 0.05 to 10.0 ng/mL.</p><h5><strong>PFAS detection in real cosmetic samples&nbsp;</strong></h5><p>Of the 15 cosmetic products assessed, one eyeshadow sample was detected with a trace level of positive PFAS analytes, including PFHeA, PFHpA, PFNA, and PFOA, at about 0.1 ng/g in the sample. Figure 5 shows the method blanks and positive detection of PFHpA and PFNA in this eyeshadow&nbsp;product.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>A simplified, rapid, and reliable method using QuEChERS extraction followed by EMR mixed-mode passthrough cleanup using the Agilent Captiva EMR PFAS Food II cartridge and LC/MS/MS detection was developed for 41 PFAS analysis in cosmetics.</p><h3><strong>2. Metrohm: brightRC – Advanced brightener analysis with a response curve</strong></h3><p><strong>Next-level brightener determination in nonlinear systems</strong></p><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_v_242_download_83376b2923.pdf"><strong>Full PDF for download</strong></a></li></ul><h4><strong>WHAT IS BRIGHTRC?&nbsp;</strong></h4><p>brightRC is a response curve calibration approach for the determination of brightener-type additives by CVS or CPVS (Cyclic Pulse Voltammetric Stripping), hence the name «brightRC». This technique is suitable to measure brightener content in copper baths for plating. The method is based on signal normalization using the electrolyte value, which is the response for the virgin makeup solution (VMS) saturated with suppressor, and brightener defined as Q0 in the viva software. All recorded signals are divided by Q0 and the normalized values are plotted against concentration to generate an external calibration curve. This calibration can then be used for routine sample determination without repeated standard additions.&nbsp;</p><p>The sample is simply measured using the same method as the calibration, signals are normalized and evaluated using the mathematically fitted calibration curve. By decoupling calibration from sample measurement, brightRC removes the need for repeated standard additions with each sample measurement, enabling faster and more efficient routine analysis.&nbsp;</p><p>The brightRC calibration approach supports flexible regression models including quadratic and nonlinear regression. This allows reliable quantification of additive systems that do not exhibit a wide linear working range or exhibit nonlinear response characteristics, thereby expanding the range of applicable brightener chemistries.&nbsp;</p><p>One important detail that further differentiates brightRC from the MLAT approach relates to normalization of the signal for calibration and sample analysis. This normalization approach improves measurement robustness as variations in absolute signal intensity naturally occurring over the working day are mathematically compensated.</p><h4><strong>HOW DOES BRIGHTRC WORK IN PRACTICE?&nbsp;</strong></h4><p>The brightRC workflow separates calibration and sample analysis. It can be implemented in different setups (from manual to fully automated) depending on the additive system and concentration range. After recording the electrolyte value, calibration standards are measured to establish the calibration curve, otherwise known as the response curve. Once the calibration is stored, samples are determined with the same CVS or CPVS method, without further standard additions. The sample concentration is automatically calculated from the normalized signal using the calibration curve.&nbsp;</p><p>For sample measurement, viva 4.0 provides two practical approaches:&nbsp;</p><ol><li>«brightRC with solution exchange»&nbsp;</li><li>«brightRC with sample dilution» Both variants follow the same calibration principle and differ only in the sample handling strategy.</li></ol><h4><strong>WHEN TO USE BRIGHTRC?&nbsp;</strong></h4><p>brightRC is ideal for routine brightener control in stable plating baths where high sample throughput is required. It is particularly suitable when matrix conditions are consistent and electrode fouling is limited. The approach is especially advantageous for additive systems exhibiting nonlinear signal behavior. If strong matrix variations, significant breakdown products, or severe electrode fouling are expected and a linear correlation between signal and analyte concentration is guaranteed, standard addition techniques such as MLAT are preferred because they may provide additional robustness.</p><h3><strong>3. Shimadzu / ASMS: PFAS Analysis of Leachate: Evaluating Key Sample Preparation and LC–MS/MS Parameters for Improved Performance</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/WP_255_PFAS_Analysis_of_Leachate_Evaluating_Key_Sample_Preparation_and_LC_MSMS_Parameters_for_Improved_Performance_1114ee264c.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Landfill leachate is a highly complex and variable matrix that poses significant challenges for the analysis of per- and polyfluoroalkyl substances (PFAS). Although U.S. Environmental Protection Agency Method 1633A provides a standardized framework for PFAS quantitation,1 analytical performance remains strongly influenced by sample preparation, chromatographic separation, and mass spectrometric conditions. Landfill leachate samples were extracted using WAX SPE following EPA 1633A and analyzed by LC–MS/MS using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8060"><strong>Shimadzu LCMS-8060</strong></a><strong> </strong>under negative ESI conditions. This study evaluates the key factors governing LC–MS/MS sensitivity, signal response, and reproducibility in landfill leachate, including a systematic assessment of SPE, chromatographic behavior, and ionsource conditions across PFAS classes (Figure 1).</p><h4><strong>Results&nbsp;</strong></h4><p>Chromatographic performance was evaluated across four C18 columns (A–D) using peak area, height, asymmetry, and HETP. Two columns produced a higher signal and improved peak shape, while the remaining columns showed greater efficiency. Under repeated batches followed by storage in acetonitrile, the Shimadzu GIST column produced a consistent signal (≤15% change), whereas another column showed 20–50% signal loss, particularly for long-chain PFAS and precursors, indicating greater susceptibility to matrix accumulation (Figure 2).</p><h4><strong>Conclusion&nbsp;</strong></h4><ul><li>PFAS analytical performance was dependent on compoundclass-dependent extraction, chromatography, and ionization behavior.&nbsp;</li><li>GIST demonstrated lower signal variability under repeated matrix exposure, with lower signal degradation than alternative C18 phases.</li><li>Neutral PFAS showed poor retention on WAX sorbents, requiring optimization beyond standard EPA 1633A extraction conditions.&nbsp;</li><li>PFCA response was more influenced by gas flow conditions, whereas PFSA response was more sensitive to source temperature conditions.</li></ul><h3><strong>4. Thermo Fisher Scientific / ASMS: Ultra-high throughput proteomics at &gt;500 samples/day using advanced </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398"><strong>Vanquish Neo UHPLC</strong></a><strong> methods with </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959"><strong>Orbitrap Astral Zoom MS</strong></a></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004708_lsms_ultra_high_throughput_vanquish_astral_proteomics_po004708_asms2026_na_en_f6499c3eec.pdf"><strong>Full PDF for download</strong></a></li></ul><p>High-throughput mass spectrometry is increasingly critical for large-cohort proteomics, population-scale studies, and screening applications. While established LC–MS platforms routinely support several hundred samples per day, further increases in throughput are often limited by chromatographic stability, robustness, and reproducibility under ultra-short gradient conditions, as well as the acquisition speed of the mass spectrometer. Here, we describe an advanced ultra-high-throughput LC–MS methodology developed on the Thermo Scientific Vanquish Neo UHPLC system and Thermo Scientific Orbitrap Astral Zoom mass spectrometer. The goal of this work was to extend the practical throughput limits of LC–MS while achieving deep proteome coverage and maintaining run-to-run reproducibility.</p><h4><strong>Materials and methods</strong></h4><h5><strong>LC-MS analysis&nbsp;</strong></h5><p>Samples were separated on a Thermo Scientific 150 µm × 70 mm C18 column (prototype, not commercially available yet) operated at 4 µL/min using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398"><strong>Vanquish Neo UHPLC system</strong></a> in NanoCap Trap&amp;Elute configuration at room temperature. Prototype methods allowing for reduced LC duty cycles (approx. 1 min of sample pickup/loading and integrated trap-column offline wash) were applied, the standard gradients were adjusted to 1 – 3 min. The Thermo Scientific PepMap Neo 5 µm C18 300 µm × 5 mm cartridge was used as trap column and the separation column connected with a 15 µm ID capillary Thermo Scientific EASY-Spray emitter (ES994) to an EASY-Spray source. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959"><strong>Orbitrap Astral Zoom MS</strong></a> (ICSW AST 2.2) was operated in a dataindependent acquisition (DIA) mode, MS method parameters are given in Table 1. Comparative data for the chemoproteomics samples at lower throughputs (60-300 SPD) were acquired using an EASY-Spray 150 µm × 150 mm C18 column (ES906), as described before.&nbsp;</p><h5><strong>Data analysis&nbsp;</strong></h5><p>The raw data files were processed with Spectronaut® 20.5 software (Biognosys AG), <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782"><strong>Thermo Scientific Proteome Discoverer </strong></a>3.3 SP1 with <strong>CHIMERYS 5.0 </strong>or DIA-NN Enterprise 2.3.2 (Aptila Biotech GmbH) nodes using default settings as specified in the individual figures. The data was searched against the Homo sapiens SwissProt database (TaxID=9606: 42,252 sequences) and contaminants FASTA file. Peptide numbers correspond to unique stripped peptides</p><h4><strong>Conclusions&nbsp;</strong></h4><ul><li>The advanced Vanquish Neo UHPLC methodology in combination with the Orbitrap Astral Zoom MS delivered highly reproducible, deep and precise proteome profiling across ultra-high-throughput regimes (600 SPD) exceeding current field benchmarks.&nbsp;</li><li>The workflow enables rapid chemoproteomic screening, as demonstrated with activity-based protein profiling (ABPP) samples, reliably identifying enriched putative target proteins of an antibiotic small molecule probe.</li></ul><h3><strong>5. Waters Corporation / ASMS: Assessing Relative Response of Four European-Regulated PFAS in Human Serum Using Cyclic Ion Mobility MS</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009410_c98e7c87ec.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>The poster presents an LC-Cyclic-IMS-MS approach for assessing four European-regulated PFAS—PFOS, PFOA, PFNA, and PFHxS—in human serum. The study focuses on environmental exposure monitoring in Ghanaian firefighters and e-waste handlers, using ion mobility mass spectrometry to add separation power and improve identification confidence in complex biological matrices. The work is motivated by increasing regulatory concern around PFAS toxicity, bioaccumulation, and the need for reliable exposure assessment in human biofluids.</p><p>The experimental workflow combined reversed-phase LC with a quadrupole-cyclic ion mobility-time-of-flight MS system (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/202"><strong>SELECT SERIES</strong> <strong>Cyclic IMS</strong></a>). Human serum samples were prepared using SPE µElution plates with a polymeric reversed-phase/weak anion exchange sorbent. The LC system was modified with PFAS-free components and an isolator column to reduce background contamination, which is especially important for trace-level PFAS analysis. Identification was supported by retention time, accurate mass, isotopically labelled internal standards, and collision cross section values.</p><p>The results showed detectable levels of the four regulated PFAS in both occupational groups, with a combined detection rate of 83% across human serum samples and NIST reference standards. Firefighter samples generally showed higher concentration ranges than e-waste handler samples, especially for PFOS, PFOA, PFNA, and PFHxS. For example, PFNA reached up to 5.713 ng/mL in firefighter serum, compared with up to 1.513 ng/mL in e-waste handler serum.</p><p>Overall, the poster concludes that LC-Cyclic-IMS-MS provides a highly specific strategy for PFAS analysis in complex biological samples. Ion mobility and CCS values improve confidence in trace-level identification and help distinguish target PFAS from co-eluting matrix background. The authors suggest that this approach can support both targeted PFAS monitoring and broader non-targeted screening of known and emerging PFAS, while also helping future studies connect exposure data with environmental fate and potential toxicological effects.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 28, 2026

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A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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<p><strong>We invite you to join a hybrid event on October 15<sup>th</sup> to explore the latest solutions and workflows for overcoming environmental and food contaminant testing challenges.</strong></p><p>This event will be broadcast live from the laboratory at Thermo Fisher Scientific Customer Solutions Center (CSC) in Paris. You can join in person at one of our many 'satellite' venues worldwide, where we will offer an extended agenda, or participate remotely through the live broadcast.</p><p>During the live stream broadcast, you will meet our Customer Solution Centre specialist team and leading industry expert, John Quick, as they discuss innovative solutions to meet the ever-evolving testing demands. There will be plenty of time to ask these experts your questions.</p><p>The following sessions will be streamed all over Europe and beyond:</p><ul><li><strong>Detecting PFAS in drinking water to meet regulations across Europe</strong> – New automated sample preparation high-resolution MS solution to achieve parts per trillion detection limits.<br><i>Aristide Ganci,&nbsp;Senior Product Application Specialist, Thermo Fisher Scientific; Valérie Thibert, Senior Product Application Specialist, Thermo Fisher Scientific; Bénédicte Gauriat, Senior Product Application Specialist, Thermo Fisher Scientific</i></li><li><strong>New ready-to-go pesticide residue solution for faster, more confident results</strong><br><i>Zoe Julian, Product Marketing Specialist, Manager; Bénédicte Gauriat, Senior Product Application Specialist, Thermo Fisher Scientific; Julie Moriceau, Senior Product Application Specialist, Thermo Fisher Scientific</i></li><li><strong>Low-level contaminant testing in environmental water matrices</strong>&nbsp;– High-Resolution Accurate Mass GC-MS approach to meet future regulations<br><i>John Quick, Principal Scientist, ALS Environmental Ltd</i></li></ul><p>After the live stream concludes, 'satellite' locations offer a unique program onsite. Take this unique opportunity to connect with industry professionals, or join us to explore, network, and collaborate on your future projects. Scroll below to the venue of your choice to discover the individual agenda.</p><p>Participation is free of charge, registration is required.</p><p>For any questions about this event or regarding your registration, please email <a href="mailto:CMDEvents@thermofisher.com">CMDEvents@thermofisher.com</a>.</p><h3><strong>Preliminary agenda</strong></h3><h5><strong>10:15 Live streaming of this event to ‘satellite’ locations and online audience</strong></h5><h5><strong>10:30 Introduction</strong></h5><ul><li>Jeff Stubbs (Business Development Manager, Environment and Food Safety, Thermo Fisher Scientific)</li></ul><h5><strong>10:55 Detecting PFAS in drinking water to meet regulations across Europe - New automated sample preparation high-resolution MS solution to achieve ppt detection limits</strong></h5><ul><li>A Ganci, V Thibert and B Gauriat (Senior Product Application Specialists, Thermo Fisher Scientific)</li></ul><h5><strong>11:35 Break</strong></h5><h5><strong>12:05 New ready-to-go pesticide residue solution for faster, more confident results</strong></h5><ul><li>Z Julian, Product Marketing, B Gauriat, and J Moriceau (Senior Product Application Specialists Thermo Fisher Scientific)</li></ul><h5><strong>12:45 Low-level contaminant testing in environmental water matrices – high-resolution GCMS approach to meet future regulations</strong></h5><ul><li>John Quick (Principal Scientist, ALS Environmental Ltd)</li></ul><h5><strong>13:15 Closing comments</strong></h5><h5><strong>13:30 End of live streaming</strong></h5><div><div container-id="cq-colctrl-lt7" comp-id="B1"><div><div><div><div><div><div><blockquote><h3>How to attend?</h3><p>3 ways of attending:</p><p><strong>Customer Solution Centre (in-person event):&nbsp;</strong><br>Join the heart of the event at our Customer Solution Center in Paris, where the main agenda will be hosted and live-streamed worldwide to our ‘Satellite’ locations and our audience.</p><p><strong>Join a ‘Satellite’ location (in-person event):&nbsp;</strong><br>Our in-person ‘Satellite’ events will broadcast the main agenda live-streamed from our Customer Solution Center in Paris and supplement the day with local presentations, lab tours, or workshops. Take this opportunity to meet your Thermo Fisher representatives and industry experts from the region. Discover the individual agenda for your ‘Satellite’ location of interest.</p><p><strong>Online attendees (remote):&nbsp;</strong><br>Unable to travel to a ‘Satellite’ event? Not a problem! You can register to attend conveniently online from your desk or anywhere else through the live stream from our Customer Solutions Center in Paris.</p></blockquote></div></div></div></div></div></div></div></div>

Thermo Scientific: Solutions for environmental and food contaminant testing

Solutions for environmental and food contaminant testing

New and advancing workflows to meet and exceed European regulations.

<p><strong>This webinar is about single quad mass detectors. We will look at the development of single quads together and highlight the possibilities offered by today's single quads and how they support the user.</strong></p><p>The aim of the webinar is to show users how they can easily add mass-selective confirmation to their HPLC-UV methods. In other words: achieve greater security without more complexity.</p><blockquote><p><strong>Presenter:</strong> Shaun Pritchard (Product Specialist Liquid Phase Separations, Agilent Technologies)</p><p>Shaun Pritchard is a Product Specialist for separation products at Agilent Technologies covering the UK and Ireland. He started his career in 1989 working at a large Pharmaceutical company holding a number of roles, this is where he became passionate about HPLC finally in 1998 he took the leap to leave the lab and move to the commercial side of things and has had a number of roles within the industry until 2017 he joined Agilent.</p></blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Single Quad mass detection for Chromatographers

We will look at the development of single quads together and highlight the possibilities offered by today's single quads and how they support the user.

<p><strong>Researchers in early antibody and protein drug development are now harnessing the power of automation to transform traditional purification techniques into high-throughput, automated processes.</strong></p><p>This game-changing approach lets them explore protein expression, stability, and functionality before scaling up to larger volumes. While protein scientists excel in engineering and purification, the complexities of automation bring new challenges that can significantly impact workflow and performance.</p><p>Join us for an exciting webinar by Biotage, where we’ll dive into the evolution of major automated, small-scale protein purification platforms over the past 20 years. By focusing on screening and process development applications, you’ll gain invaluable insights that will help you choose the perfect automation platform for your needs. We’ll cover everything from entry barriers (such as time and cost) to automation efficiency, downstream compatibility, and overall performance. Plus, we’ll present comparative performance data across platforms, highlighting key metrics for optimizing protein purification such as yield, concentration, consistency, and purity.</p><h4><strong>What you will learn:</strong></h4><ul><li><strong>Valuable insights into how automation platforms are used in screening and process development applications</strong><br>&nbsp;</li><li><strong>Tips on selecting the appropriate automation platform for your specific needs</strong><br>&nbsp;</li><li><strong>Understanding the time and cost barriers to adopting automation</strong><br>&nbsp;</li><li><strong>Evaluating the efficiency of different automation platforms and assessing how well they integrate with downstream processes</strong><br>&nbsp;</li><li><strong>Comparative performance data across the platforms – protein yield, concentration, consistency, and purity</strong></li></ul><h4><strong>Who should attend:</strong></h4><ul><li>Protein Scientists: Those involved in protein engineering and purification</li><li>Biotech Researchers: Professionals working on antibody and protein drug development</li><li>Research Scientists: Individuals focusing on high-throughput screening and process development</li><li>Lab Managers: Those overseeing laboratory workflows and looking to integrate automation</li></ul><blockquote><p><strong>Presenter: </strong>Gabrielle Kerkow (Application Scientist, Biotage)</p><p>Gabrielle Kerkow earned her Bachelor of Science in Biological Engineering and Psychology from Louisiana State University and is currently pursuing a Master of Biotechnology at San Jose State University. Having experience with chromatography, she joined Biotage as an Application Scientist, focusing on automating protein and plasmid purification on liquid handlers. Her role involves method development, programming for high-throughput purification, and evaluating the performance of protein purification.</p></blockquote>

Separation Science: Elevating Protein Purification Efficiency: Transitioning from Manual Techniques to Automated Platforms

Elevating Protein Purification Efficiency: Transitioning from Manual Techniques to Automated Platforms

The complexities of automation bring new challenges that can significantly impact workflow and performance.

Elevating Protein Purification Efficiency

<p>Key takeaways from this webinar include:</p><ul><li><strong>Understanding surfactants and their significance</strong></li><li><strong>How to accurately measure cationic, anionic, and non-ionic surfactants using potentiometric titration</strong></li><li><strong>The distinction between aqueous surfactant titration and two-phase surfactant titration, and when to use each method</strong></li><li><strong>How two-phase surfactant titration serves as a viable alternative to the widely used Epton Titration</strong></li><li><strong>Choosing the right potentiometric sensor for specific titrations</strong></li></ul><blockquote><p><strong>Presenter: </strong>Mona Wagner (Application Specialist Titration, to learn how to analyze surfactants by automatic potentiometric titration)</p></blockquote>

Metrohm: Best practice for surfactant analysis by potentiometric titration

Best practice for surfactant analysis by potentiometric titration

How to accurately measure cationic, anionic, and non-ionic surfactants using potentiometric titration.

Best practice for surfactant analysis

<p><strong>Join us as we introduce our path to visualize molecules from tissue at single cell resolution using DESI MS imaging, with the commercial DESI XS ionization source coupled to Xevo G3 QToF Mass Spectrometer.</strong></p><p>This webinar will explain how to easily transition to high resolution MS Imaging with a traditional entry level commercial DESI MS imaging toolkit.</p><h4>Learn how to:</h4><ul><li><strong>Understand High spatial resolution MS Imaging is now accessible to all labs and applications</strong></li><li><strong>Access single cell DESI Imaging with the combination of DESI XS and Xevo G3 QToF</strong></li><li><strong>Leverage both high sensitivity and high speed of acquisition with the Xevo G3 QToF without compromising MS Imaging data quality</strong></li><li><strong>Utilize HDI 1.8 Imaging software for microscope mode analysis for effective use of time in MS Imaging experiments</strong></li><li><strong>Obtain comprehensive and detailed spatial information with limited sample preparationAdd MSI analysis into a multi-modal imaging workflow with ease</strong></li></ul><blockquote><p><strong>Presenter:&nbsp;</strong>Dr Jamie Reilly (Postdoctoral Researcher, University of Galway)</p><p>Dr. Jamie Reilly is a postdoctoral researcher in NUIG Galway. Prior to joining the CELLIMAGE project, Jamie undertook his PhD in Regenerative Medicine in REMEDI, where he used bioinformatics to analyze molecular pathways in cellular and animal models of autism spectrum disorder (ASD). Jamie attended Trinity College Dublin (TCD) for his undergraduate degree in Genetics, and has a Masters in Biotechnology from Queens University Belfast.</p><p><strong>Presenter:&nbsp;</strong>Dr. Mark Towers (Principal Scientist - MS Technology, Waters)</p><p>Mark Towers gained his PhD from the University of Reading in Bioanalytical chemistry on the Development of applications and techniques for UV-Liquid-MALDI-MS. Mark joined Waters over 10 years ago into the Scientific operations team as a senior scientist, working on high resolution mass spectrometry and imaging.</p><p>Mark’s primary area of focus has been surface analysis by desorption electrospray ionisation (DESI) with over 7 years experience and matrix assisted laser desorption ionisation for over 14 years, principally but not limited to tissue sections for the detection and distribution mapping of a wide range of molecules.</p><p><strong>Presenter: </strong>Dr. Joanne Ballantyne (Director, MS Imaging program, Waters Corporation)</p><p>Joanne completed a PhD in Mass Spectrometry at UMIST in Manchester 2002, in collaboration with UK Ministry of Defence's Science and Technology Lab (Dstl). This was followed by a post-doc at NIH in Bethesda, USA, working in OMICS for the NIMH. After returning to the UK, and time as a MALDI applications chemist at Shimadzu Biotech, Joanne joined Waters Corporation in 2007 as a member of the OMICS Application team in Manchester and progressed to managing the team. Following this Joanne spent a number of years in Business Development and Field Marketing for discovery applications in the Northern European region. At the end of 2018 Joanne joined the Product Management group with responsibility for the SYNAPT and MS Imaging technologies. After gaining ILM7 accreditation in strategic leadership, Joanne is now Director of the MS Imaging program.</p></blockquote>

Waters Corporation: Towards Single Cell Imaging Session 1

Towards Single Cell Imaging Session 1

How to easily transition to high resolution MS Imaging with a traditional entry level commercial DESI MS imaging toolkit.

<p><strong>Dive into the future of Karl Fischer titration in this engaging webinar, where we will unveil the cutting-edge advancements and emerging trends shaping this critical analytical field.</strong></p><p>This session is specifically designed to highlight the innovative features of the EVA KF Titrators, which represent the forefront of titration technology. We will explore the integration of advanced technologies such as the Fast-Forecasting Amperometric (FFA™) Control Algorithm, which enhances the speed and reliability of water content determination, drastically reducing titration times without compromising result quality. Learn about the advanced drift management techniques that ensure consistent and reliable measurements, critical for industries where accuracy is paramount.</p><p>Participants will also gain insight into how these technologies are being applied across various sectors, driving improvements in everything from pharmaceuticals to food and chemical manufacturing. The session will cover how the flexibility of the EVA KF titrators enables laboratories to adapt quickly to new regulations and testing requirements, supporting sustained compliance and operational excellence.</p><h4>Program Overview</h4><ul><li><strong>Technological Innovations: Uncover the cutting-edge advancements in Karl Fischer titration, including the integration of the Fast-Forecasting Amperometric (FFA™) Control Algorithm.</strong></li><li><strong>Precision and Efficiency: Learn about new drift management techniques that enhance the accuracy and reliability of measurements, crucial for industries where precision is paramount.</strong></li><li><strong>Adaptability to Regulations: Explore how EVA KF Titrators' flexibility aids laboratories in swiftly adapting to new regulations and maintaining operational excellence.</strong></li></ul><blockquote><p><strong>Presenter: </strong>Erik Gubler</p><p>A Global Product Manager at METTER TOLEDO in Switzerland, specializing in analytical instruments. He holds a master's degree in chemistry from the Swiss Federal Institute of Technology (ETH Zurich). He has been an integral part of developing the next generation of automated titrators and software. His chemistry expertise and product insight ensure the continuous advancement and reliability of METTLER TOLEDO’s titrators across various industries.</p><p><strong>Presenter:&nbsp;</strong>Lukas Fritsche</p><p>An Application Specialist at METTLER TOLEDO in Switzerland, specializing in titration technology. With a Ph.D. in chemistry from ETH Zurich, Lukas plays a pivotal role at METTLER TOLEDO in advancing application development and enhancing instrument capabilities. His work supports the continuous innovation and reliability of the company’s analytical instruments.</p></blockquote>

Mettler Toledo: The Future of Karl Fischer Titration

The Future of Karl Fischer Titration

The innovative features of the EVA KF Titrators, which represent the forefront of titration technology.

<p><strong>In this webinar, we will present several case studies of how different light scattering techniques can be used for advanced characterization of biopharmaceutical samples.</strong></p><p>In particular, we will focus on dynamic light scattering (DLS), size-exclusion chromatography coupled to multi-angle light scattering (SEC-MALS) and real-time multi-angle light scattering (RT-MALS).</p><h4>Key Takeaways:</h4><ul><li><strong>Learn basics of light scattering techniques</strong><br>&nbsp;</li><li><strong>Distinguish between different light scattering methods – DLS, SLS, SEC-MALS, RT-MALS</strong><br>&nbsp;</li><li><strong>Understand how different light scattering can be used to characterize therapeutic proteins</strong></li></ul><h4>Who should attend:</h4><ul><li>Scientists interested in analytical characterization of biopharmaceuticals using light scattering techniques.</li></ul><blockquote><p><strong>Presenter: </strong>Hristo L. Svilenov, Ph.D. (Ghent University)</p><p>Dr. Svilenov is an Associate Professor in Biopharmaceutical Technology. His research interests are in the development, formulation and manufacturing aspects of biotherapeutics. Previously, Dr. Svilenov was a postdoctoral researcher funded by a Peter and Traudl Engelhorn Fellowship at the TU Munich. From 2016 to 2019, Dr. Svilenov did his PhD supported by a Marie Sklodowska-Curie Fellowship at the Ludwig Maximilian University of Munich. He defended his doctoral thesis with the highest distinctions in Germany (summa cum laude) and received the AbbVie prize and the Carl-Wilhelm-Scheele of the German Pharmaceutical Society for his work.</p><p><strong>Presenter:&nbsp;</strong>Lorenzo Gentiluomo, Ph.D. (Waters | Wyatt Technology)</p><p>Lorenzo Gentiluomo, Ph.D. in Pharmaceutical Technology and currently concluding an Executive MBA, has several years of experience in drug product development. He has successfully managed the development of 26 biological drug candidates, including 3 BLA projects, during his tenure at Coriolis and Lonza. Lorenzo now serves as Senior Account Sales Manager for Wyatt Technology, overseeing the portfolio in Switzerland, where he applies his deep technical expertise to support and expand the use of advanced analytical solutions in the biopharmaceutical industry.</p></blockquote>

Waters Corporation: Leveraging light scattering techniques for enhanced biotherapeutic characterization and development

Leveraging light scattering techniques for enhanced biotherapeutic characterization and development

How different light scattering techniques can be used for advanced characterization of biopharmaceutical samples.

Leveraging light scattering techniques

<p><strong>Whether you are developing new materials for batteries electrodes, testing full or half cells or working on new electrolytes, electrochemical methods are at the core of your daily activities.&nbsp;</strong></p><p>In this webinar we will cover theoretical and practical aspects of various electrochemical techniques for battery research and testing. From the most fundamental CC cycling, to more advanced &nbsp;techniques such as galvanostatic intermittent titration technique (GITT), this webinar is meant to help you navigate this field and chose with confidence with techniques are most fit for your research.</p><p>This webinar is accessible to anyone working in the field of battery, materials research, with or without a background in electrochemistry.</p><blockquote><p><strong>Presenter: </strong>Thomas Touzalin, PhD.&nbsp;</p><p>Product Manager at Metrohm Autolab, helping the electrochemistry researchers achieve their goals by providing the right solution for any research problems as well as offering technical support for the Autolab product range.</p></blockquote>

Metrohm: Electrochemical techniques for battery research and testing

Electrochemical techniques for battery research and testing

Theoretical and practical aspects of various electrochemical techniques for battery research and testing.

<p><strong>This webinar will guide you through optimizing your Q-TOF for superior data quality by selecting the most suitable tune mode for your samples.</strong></p><p>We will delve into various MS acquisition modes, including MS only, All Ions (QRAI), Auto MS/MS (directed MS/MS; targeted MS/MS), and PRM mode.</p><p>Learn how to fine-tune method settings to enhance your data quality and achieve the best analytical results. Whether you’re a seasoned user or new to QTOF, this session will provide valuable insights to elevate your mass spectrometry.</p><blockquote><p><strong>Presenter:</strong> &nbsp;Stefan Schaebler (Application Specialist LC/MS, Agilent Technologies)</p><p>Stefan completed his PhD in Biology, specializing in LC-MS-based lipidomics and metabolomics. Following his doctoral studies, he joined Agilent Technologies as an LC/MS Application Specialist. In this role, Stefan focuses on small molecule applications using LC-QTOF, with a strong emphasis on software tools and data interpretation.</p></blockquote>

Agilent Technologies: MassHunter Masterclass Months

MassHunter Acquisition Q-TOF Part II – Advance Settings

Join us for an in-depth exploration of advanced settings in MassHunter Acquisition for Q-TOF systems.

<p><strong>Novel biotherapeutics, including bispecific and multispecific antibodies (bsAb, msAb), antibody-drug conjugates (ADCs), and antibody-oligonucleotide conjugates (AOCs), are revolutionizing biomedical therapies yet pose analytical challenges due to their sequence diversity, structural complexity, and susceptibility to various degradation pathways.</strong></p><p>In this presentation, Ben Niu will showcase how his team has integrated advanced techniques such as automation and machine learning to achieve unprecedented mass spectrometry-focused sample handling capabilities and rapid turnover rates. These approaches deliver unmatched depth and breadth in understanding diverse molecular modalities within a condensed timeline.</p><p><strong>Who should attend?</strong></p><p>Scientists who develop novel biotherapeutics</p><p><strong>What You Will Learn?</strong></p><p>Overcoming analytical challenges relating to the development of novel biotherapeutics, via utilization of automation and machine learning to achieve unprecedented mass spectrometry-focused sample handling capabilities and rapid turnover rates.</p><blockquote><p><strong>Presenter: </strong>Ben Niu, PhD (Principal Scientist, Discovery Biotherapeutics, Bristol Myers Squibb)</p><p>Ben Niu is a Principal Scientist at Bristol Myers Squibb (BMS), leading a pioneering mass spectrometry team within the Discovery Biotherapeutics department. With over seven years of experience in the biopharmaceutical industry, Ben specializes in analytical characterization, particularly in mass spectrometry techniques, driving critical insights for drug development. He champions the integration of automation and machine learning to revolutionize analytical workflows.</p><p><strong>Presenter:</strong> Dr. Kelly Broster (Sr Manager, Market Development &amp; Collaborations, Pharma Biopharma, Thermo Fisher Scientific)</p></blockquote>

Thermo Fisher Scientific: High-throughput and automation in novel modalities (msAbs, ADCs, AOCs) screening, characterization, and beyond

High-throughput and automation in novel modalities (msAbs, ADCs, AOCs) screening, characterization, and beyond

Integrated advanced techniques to achieve unprecedented mass spectrometry-focused sample handling capabilities and rapid turnover rates