Modernizing Routine HPLC Methods: Aligning Productivity, Compliance, and Sustainability

<h3>How to Easily Adapt Your Existing HPLC Methods</h3>Developing and optimizing an HPLC method is essential for achieving reliable separations, accurate quantification, and reproducible analytical results. As chromatography technology advances, one of the most common optimization strategies is transferring established methods to modern columns with smaller dimensions.Although the benefits are clear, many analysts hesitate when it comes to modifying a validated method.<blockquote>Questions quickly arise: How should the flow rate be adjusted? What injection volume is appropriate? Does the gradient need to be recalculated? Will the retention times change?</blockquote>Even with the necessary equations available, performing all these calculations manually can be time-consuming and prone to errors. Instead of simplifying the workflow, method transfer often becomes an unnecessary obstacle.Fortunately, there is an easier solution.<h4>Simplify Your HPLC Method Transfer</h4>Moving an existing method to a different column does not have to be complicated.The KNAUER HPLC Method Converter is designed to automate the calculations required for method transfer. Simply enter the parameters of your current method, including the original column dimensions, and specify the dimensions of the new column—length, internal diameter, and particle size. The software instantly calculates the recommended flow rate and injection volume for the new conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_7f7c19f7a3.webp" alt="KNAUER: HPLC Method Converter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_7f7c19f7a3.webp 210w," sizes="100vw" width="1022" height="759">The next step is equally straightforward. Copy the original gradient profile into the converter, and the software automatically generates the corresponding gradient program for the new column.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_2_8ba46e78bc.webp" alt="KNAUER: HPLC Method Converter - Gradients" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_2_8ba46e78bc.webp 195w," sizes="100vw" width="1440" height="1150">For even greater confidence, the converter also allows you to enter chromatographic peak information. Based on these data, it predicts the expected retention times under the new conditions. While experimental verification on the HPLC system is still recommended, these predictions provide an excellent starting point for method optimization.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_3_34f16bb3bf.webp" alt="KNAUER: HPLC Method Converter - Chromatograms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_3_34f16bb3bf.webp 196w," sizes="100vw" width="1024" height="814">The converter was developed with one primary objective: making HPLC method adaptation faster, easier, and more reliable while reducing the effort required from the user. Its intuitive interface, modern design, and automatic calculations help save valuable laboratory time—and it is available free of charge.<h4>Modernize Your Methods and Improve Laboratory Efficiency</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_4_31e7188db4.webp" alt="KNAUER: Modernize Your Methods and Improve Laboratory Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_4_31e7188db4.webp 245w," sizes="100vw" width="720" height="449"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_5_815a7bf889.webp" alt="KNAUER: HPLC Method Converter - Results" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_5_815a7bf889.webp 194w," sizes="100vw" width="1440" height="1157">One of the major advantages of the KNAUER HPLC Method Converter is its compliance with USP General Chapter &lt;621&gt;.For pharmaceutical laboratories, chromatographic methods must comply with pharmacopeial requirements that ensure product quality and patient safety. Historically, many USP methods were developed using larger HPLC columns that no longer reflect the capabilities of modern chromatography systems.Current USP, European Pharmacopoeia (Ph. Eur.), and Japanese Pharmacopoeia (JP) guidelines now permit specific adjustments to column dimensions and particle size for both isocratic and gradient methods. These changes are allowed provided that defined relationships between chromatographic parameters are maintained.The KNAUER Method Converter automatically checks whether the selected column dimensions remain within the permitted USP limits. If the proposed changes comply with the guidelines, the software confirms that the method can be transferred while remaining pharmacopeia compliant. This removes uncertainty from the method conversion process and helps laboratories modernize their analytical workflows with confidence.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_6_c9d1e28c54.webp" alt="Unsplash: Illustrative picture" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_6_c9d1e28c54.webp 245w," sizes="100vw" width="720" height="449"><h4>Increase Productivity While Reducing Solvent Consumption</h4>Updating HPLC methods is not only about shortening analysis times—it also supports more sustainable laboratory practices.Using shorter columns together with optimized flow rates significantly reduces solvent consumption, lowering operating costs and decreasing the amount of hazardous chemical waste generated during routine analyses. Faster separations also improve instrument throughput, enabling more samples to be processed within the same amount of time.Modernizing chromatographic methods therefore delivers a dual benefit: greater laboratory productivity and a smaller environmental footprint. With only a few simple adjustments, laboratories can improve efficiency while supporting more sustainable analytical workflows.

HPLC method conversion free and easy

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 6th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by Agilent Technologies and Metrohm and posters by Shimadzu/ASMS, Thermo Fisher Scientific/ASMS and Waters Corporation/ASMS!<h3>1. Agilent Technologies: Determination of 41 Per- and Polyfluoroalkyl Substances (PFAS) in Cosmetics</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_captiva_emr_pfas_food_ii_cartridge_5994_9111en_agilent_be91861646.pdf">Full PDF for download</a></li></ul>Per- and polyfluoroalkyl substances (PFAS) are synthetic compounds composed of highly stable carbon–fluorine (C–F) bonds, giving them exceptional thermal and chemical stability, leading to poor environmental degradability. PFAS have been used in cosmetics to provide water resistance, oil repellency, or application smoothness. The unintentionally presented PFAS residues in raw materials may remain in finished cosmetic products, and can be absorbed through the skin. Lip products and spray type products can also lead to inhalation or oral exposure to residues.1,2&nbsp;Agilent Captiva EMR PFAS Food cartridges are designed to provide streamlined and comprehensive matrix removal after QuEChERS extraction for complex sample matrices, demonstrating effective matrix cleanup efficiency and excellent performance for quantitative PFAS analysis.3,4 In this study, we performed QuEChERS extraction followed by cleanup with Captiva EMR PFAS Food II cartridges for PFAS analysis in five types of cosmetics.<h4>Experimental</h4><h5>Equipment and materials&nbsp;</h5>The study was performed using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III">Agilent 1290 Infinity III LC</a> system consisting of an Agilent 1290 Infinity III high-speed pump (G7120A), an Agilent 1290 Infinity III multisampler (G7167B), and an Agilent 1290 Infinity III multicolumn thermostat (G7116B). The LC system was coupled to an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6495D">Agilent 6495D triple quadrupole LC/MS system</a> (G6495D) equipped with an Agilent Jet Stream Electrospray ion source. Agilent MassHunter Workstation software was used for data acquisition and analysis.&nbsp;The 1290 Infinity III LC system was modified using an Agilent InfinityLab PFAS Analysis HPLC conversion kit (part number 5004-0006), including an Agilent InfinityLab PFC delay column, 4.6 × 30 mm (part number 5062-8100). Chromatographic separation was performed using an Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=ZORBAX+RRHD+Eclipse+Plus">ZORBAX RRHD Eclipse Plus</a> C18, 95 Å, 2.1 × 100 mm, 1.8 µm (part number 959758-902).<h4>Results and discussion&nbsp;</h4><h5>Chromatography and calibration curve linearity&nbsp;</h5>Figure 2 shows the overlaid chromatograms for 41 PFAS calibration standards, including 0.05, 0.1, 0.2, 0.5, 1.0, 2.0, 5.0, and 10.0 ng/mL in ACN. The table on the right shows the peaks identification and corresponding retention time. Across 41 PFAS compounds, R² values ranged from 0.9940 to 0.9998, indicating excellent linearity for all analytes' calibration curves across the calibration range of 0.05 to 10.0 ng/mL.<h5>PFAS detection in real cosmetic samples&nbsp;</h5>Of the 15 cosmetic products assessed, one eyeshadow sample was detected with a trace level of positive PFAS analytes, including PFHeA, PFHpA, PFNA, and PFOA, at about 0.1 ng/g in the sample. Figure 5 shows the method blanks and positive detection of PFHpA and PFNA in this eyeshadow&nbsp;product.<h4>Conclusion&nbsp;</h4>A simplified, rapid, and reliable method using QuEChERS extraction followed by EMR mixed-mode passthrough cleanup using the Agilent Captiva EMR PFAS Food II cartridge and LC/MS/MS detection was developed for 41 PFAS analysis in cosmetics.<h3>2. Metrohm: brightRC – Advanced brightener analysis with a response curve</h3>Next-level brightener determination in nonlinear systems<ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_v_242_download_83376b2923.pdf">Full PDF for download</a></li></ul><h4>WHAT IS BRIGHTRC?&nbsp;</h4>brightRC is a response curve calibration approach for the determination of brightener-type additives by CVS or CPVS (Cyclic Pulse Voltammetric Stripping), hence the name «brightRC». This technique is suitable to measure brightener content in copper baths for plating. The method is based on signal normalization using the electrolyte value, which is the response for the virgin makeup solution (VMS) saturated with suppressor, and brightener defined as Q0 in the viva software. All recorded signals are divided by Q0 and the normalized values are plotted against concentration to generate an external calibration curve. This calibration can then be used for routine sample determination without repeated standard additions.&nbsp;The sample is simply measured using the same method as the calibration, signals are normalized and evaluated using the mathematically fitted calibration curve. By decoupling calibration from sample measurement, brightRC removes the need for repeated standard additions with each sample measurement, enabling faster and more efficient routine analysis.&nbsp;The brightRC calibration approach supports flexible regression models including quadratic and nonlinear regression. This allows reliable quantification of additive systems that do not exhibit a wide linear working range or exhibit nonlinear response characteristics, thereby expanding the range of applicable brightener chemistries.&nbsp;One important detail that further differentiates brightRC from the MLAT approach relates to normalization of the signal for calibration and sample analysis. This normalization approach improves measurement robustness as variations in absolute signal intensity naturally occurring over the working day are mathematically compensated.<h4>HOW DOES BRIGHTRC WORK IN PRACTICE?&nbsp;</h4>The brightRC workflow separates calibration and sample analysis. It can be implemented in different setups (from manual to fully automated) depending on the additive system and concentration range. After recording the electrolyte value, calibration standards are measured to establish the calibration curve, otherwise known as the response curve. Once the calibration is stored, samples are determined with the same CVS or CPVS method, without further standard additions. The sample concentration is automatically calculated from the normalized signal using the calibration curve.&nbsp;For sample measurement, viva 4.0 provides two practical approaches:&nbsp;<ol><li>«brightRC with solution exchange»&nbsp;</li><li>«brightRC with sample dilution» Both variants follow the same calibration principle and differ only in the sample handling strategy.</li></ol><h4>WHEN TO USE BRIGHTRC?&nbsp;</h4>brightRC is ideal for routine brightener control in stable plating baths where high sample throughput is required. It is particularly suitable when matrix conditions are consistent and electrode fouling is limited. The approach is especially advantageous for additive systems exhibiting nonlinear signal behavior. If strong matrix variations, significant breakdown products, or severe electrode fouling are expected and a linear correlation between signal and analyte concentration is guaranteed, standard addition techniques such as MLAT are preferred because they may provide additional robustness.<h3>3. Shimadzu / ASMS: PFAS Analysis of Leachate: Evaluating Key Sample Preparation and LC–MS/MS Parameters for Improved Performance</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/WP_255_PFAS_Analysis_of_Leachate_Evaluating_Key_Sample_Preparation_and_LC_MSMS_Parameters_for_Improved_Performance_1114ee264c.pdf">Full PDF for download</a></li></ul>Landfill leachate is a highly complex and variable matrix that poses significant challenges for the analysis of per- and polyfluoroalkyl substances (PFAS). Although U.S. Environmental Protection Agency Method 1633A provides a standardized framework for PFAS quantitation,1 analytical performance remains strongly influenced by sample preparation, chromatographic separation, and mass spectrometric conditions. Landfill leachate samples were extracted using WAX SPE following EPA 1633A and analyzed by LC–MS/MS using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8060">Shimadzu LCMS-8060</a> under negative ESI conditions. This study evaluates the key factors governing LC–MS/MS sensitivity, signal response, and reproducibility in landfill leachate, including a systematic assessment of SPE, chromatographic behavior, and ionsource conditions across PFAS classes (Figure 1).<h4>Results&nbsp;</h4>Chromatographic performance was evaluated across four C18 columns (A–D) using peak area, height, asymmetry, and HETP. Two columns produced a higher signal and improved peak shape, while the remaining columns showed greater efficiency. Under repeated batches followed by storage in acetonitrile, the Shimadzu GIST column produced a consistent signal (≤15% change), whereas another column showed 20–50% signal loss, particularly for long-chain PFAS and precursors, indicating greater susceptibility to matrix accumulation (Figure 2).<h4>Conclusion&nbsp;</h4><ul><li>PFAS analytical performance was dependent on compoundclass-dependent extraction, chromatography, and ionization behavior.&nbsp;</li><li>GIST demonstrated lower signal variability under repeated matrix exposure, with lower signal degradation than alternative C18 phases.</li><li>Neutral PFAS showed poor retention on WAX sorbents, requiring optimization beyond standard EPA 1633A extraction conditions.&nbsp;</li><li>PFCA response was more influenced by gas flow conditions, whereas PFSA response was more sensitive to source temperature conditions.</li></ul><h3>4. Thermo Fisher Scientific / ASMS: Ultra-high throughput proteomics at &gt;500 samples/day using advanced <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Vanquish Neo UHPLC</a> methods with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959">Orbitrap Astral Zoom MS</a></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004708_lsms_ultra_high_throughput_vanquish_astral_proteomics_po004708_asms2026_na_en_f6499c3eec.pdf">Full PDF for download</a></li></ul>High-throughput mass spectrometry is increasingly critical for large-cohort proteomics, population-scale studies, and screening applications. While established LC–MS platforms routinely support several hundred samples per day, further increases in throughput are often limited by chromatographic stability, robustness, and reproducibility under ultra-short gradient conditions, as well as the acquisition speed of the mass spectrometer. Here, we describe an advanced ultra-high-throughput LC–MS methodology developed on the Thermo Scientific Vanquish Neo UHPLC system and Thermo Scientific Orbitrap Astral Zoom mass spectrometer. The goal of this work was to extend the practical throughput limits of LC–MS while achieving deep proteome coverage and maintaining run-to-run reproducibility.<h4>Materials and methods</h4><h5>LC-MS analysis&nbsp;</h5>Samples were separated on a Thermo Scientific 150 µm × 70 mm C18 column (prototype, not commercially available yet) operated at 4 µL/min using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Vanquish Neo UHPLC system</a> in NanoCap Trap&amp;Elute configuration at room temperature. Prototype methods allowing for reduced LC duty cycles (approx. 1 min of sample pickup/loading and integrated trap-column offline wash) were applied, the standard gradients were adjusted to 1 – 3 min. The Thermo Scientific PepMap Neo 5 µm C18 300 µm × 5 mm cartridge was used as trap column and the separation column connected with a 15 µm ID capillary Thermo Scientific EASY-Spray emitter (ES994) to an EASY-Spray source. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959">Orbitrap Astral Zoom MS</a> (ICSW AST 2.2) was operated in a dataindependent acquisition (DIA) mode, MS method parameters are given in Table 1. Comparative data for the chemoproteomics samples at lower throughputs (60-300 SPD) were acquired using an EASY-Spray 150 µm × 150 mm C18 column (ES906), as described before.&nbsp;<h5>Data analysis&nbsp;</h5>The raw data files were processed with Spectronaut® 20.5 software (Biognosys AG), <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782">Thermo Scientific Proteome Discoverer </a>3.3 SP1 with CHIMERYS 5.0 or DIA-NN Enterprise 2.3.2 (Aptila Biotech GmbH) nodes using default settings as specified in the individual figures. The data was searched against the Homo sapiens SwissProt database (TaxID=9606: 42,252 sequences) and contaminants FASTA file. Peptide numbers correspond to unique stripped peptides<h4>Conclusions&nbsp;</h4><ul><li>The advanced Vanquish Neo UHPLC methodology in combination with the Orbitrap Astral Zoom MS delivered highly reproducible, deep and precise proteome profiling across ultra-high-throughput regimes (600 SPD) exceeding current field benchmarks.&nbsp;</li><li>The workflow enables rapid chemoproteomic screening, as demonstrated with activity-based protein profiling (ABPP) samples, reliably identifying enriched putative target proteins of an antibiotic small molecule probe.</li></ul><h3>5. Waters Corporation / ASMS: Assessing Relative Response of Four European-Regulated PFAS in Human Serum Using Cyclic Ion Mobility MS</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009410_c98e7c87ec.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster presents an LC-Cyclic-IMS-MS approach for assessing four European-regulated PFAS—PFOS, PFOA, PFNA, and PFHxS—in human serum. The study focuses on environmental exposure monitoring in Ghanaian firefighters and e-waste handlers, using ion mobility mass spectrometry to add separation power and improve identification confidence in complex biological matrices. The work is motivated by increasing regulatory concern around PFAS toxicity, bioaccumulation, and the need for reliable exposure assessment in human biofluids.The experimental workflow combined reversed-phase LC with a quadrupole-cyclic ion mobility-time-of-flight MS system (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/202">SELECT SERIES Cyclic IMS</a>). Human serum samples were prepared using SPE µElution plates with a polymeric reversed-phase/weak anion exchange sorbent. The LC system was modified with PFAS-free components and an isolator column to reduce background contamination, which is especially important for trace-level PFAS analysis. Identification was supported by retention time, accurate mass, isotopically labelled internal standards, and collision cross section values.The results showed detectable levels of the four regulated PFAS in both occupational groups, with a combined detection rate of 83% across human serum samples and NIST reference standards. Firefighter samples generally showed higher concentration ranges than e-waste handler samples, especially for PFOS, PFOA, PFNA, and PFHxS. For example, PFNA reached up to 5.713 ng/mL in firefighter serum, compared with up to 1.513 ng/mL in e-waste handler serum.Overall, the poster concludes that LC-Cyclic-IMS-MS provides a highly specific strategy for PFAS analysis in complex biological samples. Ion mobility and CCS values improve confidence in trace-level identification and help distinguish target PFAS from co-eluting matrix background. The authors suggest that this approach can support both targeted PFAS monitoring and broader non-targeted screening of known and emerging PFAS, while also helping future studies connect exposure data with environmental fate and potential toxicological effects.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 28, 2026

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A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Hydrophilic interaction liquid chromatography (HILIC) and hydrophobic interaction chromatography (HIC) separations are becoming more common for a variety of applications. We see HIC being a primary method being used for antibody-drug-conjugate (ADCs) analyses and equally suitable for the analysis of protein modifications. The ability to retain neutral and ionizable polar species makes HILIC particularly popular for drug development and metabolomics studies.
This webinar will help new HILIC and HIC chromatographers avoid the common mistakes of HILIC and HIC separations by identifying the important method parameters involved. We’ll discuss how the separation works, show detailed application examples, and provide tip and tricks for robust method development.
<blockquote>
Presenter: Mohit Patel (Application Engineer, Agilent Technologies, Inc.)
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<blockquote>
Mohit Patel is part of the CSD Online AE Team. His primary focus is to support SMLC, Bio-Columns, and consumables.
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<blockquote>
Before joining Agilent, Mohit was an Assistant Professor of Chemistry at Temple University. Mohit developed his passion for science through his experiences as a faculty researcher and a mentor. Mohit actively taught, developed curriculum, and served as senior personnel, with a leadership position, on funded grants, mentored research projects, and co-coordinator of STEAM related outreach services.
</blockquote>
<blockquote>
Mohit has earned a Doctorate degree in Chemistry from Temple University in 2016.
</blockquote>

Agilent Technologies: Why Use HILIC or HIC Chromatography: Theory and Method Development Strategies

Why Use HILIC or HIC Chromatography: Theory and Method Development Strategies

This webinar will help new HILIC and HIC chromatographers avoid the common mistakes of HILIC and HIC separations.

HILIC or HIC: Theory and Method Development Strategies

In liquid chromatography, various detectors can be used. In this seminar we will take a closer look at UV, fluorescence, refractive index and ELSD detection.&nbsp;<blockquote>Presenter: Ansuman Mahato (Product Specialist Liquid Phase Separations, Agilent Technologies)Ansuman has been a Product Specialist at Agilent Technologies for 14 years, working with the pharmaceuticals, biopharmaceuticals, food, and chemicals industries. Based in Waldbronn, Germany, the manufacturing site for Agilent’s chromatography solutions, he helps make labs more efficient and compliant and has rich experience with techniques like HPLC, UHPLC, GPC/SEC, Prep LC, and 2D-LC.Ansuman is also skilled in polymer analysis using advanced tools like viscometry and MALS. He has published many papers in international journals and is a popular speaker for his practical insights and innovative solutions.</blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

HPLC Detectors

In liquid chromatography, various detectors can be used. In this seminar we will take a closer look at UV, fluorescence, refractive index and ELSD detection.

Nitrosamine Drug Substance-Related Impurities (NDSRI's) are a class of impurities that can form in drug substances and products. These impurities are of significant concern due to their potential carcinogenicity. Regulatory bodies, including the FDA, have established guidelines and acceptable intake limits to ensure the safety and efficacy of medications.NDSRI webinar series is a comprehensive three-part webinar series designed to provide valuable insights into the evolving landscape of analytical methods and regulatory requirements. This series will cover critical topics that are essential for professionals in the field.Key learnings:<ul><li>Understanding Nitrosamine / NDSRI and their impact on drug safety.</li><li>Latest regulatory guidelines and requirements.</li><li>Navigating Nitrosamines with Agilent 21 CFR compliant mass Hunter Software.</li><li>A focus on components in chromatographic challenges, with examples of NDSRI methods.</li><li>Case studies and real-world applications.</li><li>Column and consumable selection and how it influences the analysis.</li><li>Best practice for method optimization, development, and automation.</li><li>and more</li></ul><blockquote>Presenter: Ms. Preeti Bharatiya (Application Engineer - LC-MS/MS, Agilent Technologies)As an Application Engineer for LC-MS/MS at Agilent India, she specializes in supporting Pharma workflows. With a background as a Pharmacist and an MS in Pharmaceutical Sciences from NIPER, she has significant experience in the pharmaceutical application area of LC-MS/MS. Her expertise lies in drug impurity analysis, specifically in Nitrosamines quantitation using UHPLC-Triple Quadrupole as a platform. She also has experience in the pre-clinical drug development phase, including pharmacokinetic and toxicokinetic studies, drug tissue distribution studies, and rapid-automated sample preparation for biological matrices.Presenter:&nbsp;Mr. Sunil B Lakhmapure (Technical Support Specialist, Agilent Technologies)Sunil holds 19 year plus experience in the analytical field with major exposure to LC/GC with Single Quad, Triple Quad and high resolution (QToF) mass spectroscopy. Started career in Pharma R&amp;D as a scientist with hands on experience on high end instruments like LC/MS, XRD &amp; NMR. Developed &amp; published application notes using Chromatographic techniques on real world samples/issues at various levels.Presenter:&nbsp;Mr. Ankush Sharma (Regional Marketing Program Manager- APAC (Pharma &amp; Biopharma), Agilent Technologies)Ankush Sharma is a seasoned professional with over 12 years of extensive experience in sales and marketing across the pharmaceutical and biopharmaceutical sectors. His professional journey commenced with a strong educational foundation, earning a Master's degree in Biotechnology. Building upon this knowledge, he furthered his academic pursuits by obtaining a Master's in Business Administration with a specialization in Sales and Marketing, demonstrating his commitment to blending scientific understanding with strategic business acumen. For the past five years, he has been an integral part of Agilent, serving as the Regional Marketing Program Manager for the India and now Asia-Pacific (APAC) region for Pharma and Biopharma Markets.</blockquote>

Agilent: Nitrosamines & NDSRI's: Be sure with Agilent workflows

Nitrosamines & NDSRI's: Workflow readiness for Nitrosamines and NDSRI’s.

NDSRI webinar series is designed to provide valuable insights into the evolving landscape of analytical methods and regulatory requirements.

Workflow readiness for Nitrosamines and NDSRI’s.

Missed Our Annual Informatics User Conference? We’ve got you covered!&nbsp;We’re hosting a live webinar to recap the hottest topics from the conference. Don’t miss this opportunity to stay informed and engaged with the latest in Waters informatics. The webinar will include a live Q&amp;A session with our panel of experts.<h5>AGENDA INCLUDES:</h5><h4>Reviewing Data and Audit Trails in Empower</h4>In this session, we will explore best practices and essential tools for reviewing data and audit trails in Empower. You’ll gain valuable insights to streamline your data review and ensure compliance.<h4>Elevate Empower with our latest Cloud Solutions</h4>In this session we’ll explore new cloud tools designed to elevate your Empower experience. Get a sneak peek at solutions that enhance data integrity, ensure compliance, and boost lab productivity. Whether you’re an administrator, end user, lab manager, or data scientist, you’ll learn about applications that can streamline your daily tasks and simplify your workflow.<h4>LIVE Q &amp; A</h4>Engage with our panel of experts and have your pressing questions addressed during the live Q &amp; A session.<blockquote>Presenter:&nbsp;Jason Loftus (Senior Informatics Sales Development Specialist, Waters Corporation)After getting his BS in Chemistry, Jason worked as a QC Chemist for a consumer products company in Massachusetts. In 1998, he joined Waters as a Field Service Engineer based in Boston, MA. In 2001, he left Waters to join a biopharma company in Colorado, but returned to Waters in 2004 as a Field Service Engineer covering the southwestern US. In 2010, he became the Service Manager for the Mountain Region covering most of the western US. In 2016, he joined the Informatics Sales Development team and currently covers the western US, western Canada, and the southeastern US.Presenter:&nbsp;Ron Eddy (Principal Informatics Sales Development Specialist, Waters Corporation)Ron has been with Waters Corporation since 2005 when he joined the company as an Empower Sales Specialist. He has since moved into his current role supporting the Informatics sales team for Empower and NuGenesis. Prior to joining Waters, Ron worked as an analytical chemist in the pharmaceutical industry supporting the registrational phase of drug development for seven years before transitioning to a laboratory informatics role where he managed and supported various laboratory computing systems including Millennium/Empower, NuGenesis, LIMS and ELNs.Presenter:&nbsp;Shelby Lorenz (Manager, Informatics Business Development, Waters Corporation)Shelby has more than 20 years of experience in the Life Science industry. She has worked for Waters for more than 20 years and has held a variety of positions all within the Informatics organization. In her current role, she manages the Informatics Business Development team in the Americas, which has technical presales responsibility for the enterprise informatics product line including Empower, NuGenesis LMS, and waters_connect network. Prior to Waters she was a Project Manager at Creon Lab Control supporting a host of informatics products including LIMS, ELN and SDMS. Shelby holds a Bachelor of Science degree in Management Information Systems.Presenter: Lissa Wang (Informatics Business Development Specialist, Waters Corporation)Lissa has been with Waters Corporation since 1998 when she started to be a Millennium/Empower Trainer in the Customer Education department. She has also been in different roles including Technical Service and Global Service and Support for Informatics. Currently, she is part of the Informatics Business Development team supporting the Informatics sales team for Empower and NuGenesis informatics portfolio. Prior to joining Waters, Lissa worked as an analytical chemist in the pharmaceutical, biotechnology and medical device industries for 8 years across the globe and the US.</blockquote>

Waters Corporation: ICYMI (In Case You Missed It) - Empower Hot Topics from [inform] 2024

ICYMI (In Case You Missed It) - Empower Hot Topics from [inform] 2024

Best practices and essential tools for reviewing data and audit trails in Empower.

ICYMI (In Case You Missed It) - Empower Hot Topics

In this session, we explore how cutting-edge technology can detect the early signs of infectious disease with precision and speed. Early identification of disease markers can significantly enhance patient care by allowing for faster, more effective treatments.Learn how Evosep One’s robustness, sensitivity, and efficiency are helping laboratories and clinicians stay ahead of disease progression, providing key insights that lead to better health outcomes.Accurate and early detection of mycobacterial pathogens is critical for improving patient outcomes. We developed and validated multiple novel diagnostic methods. First, an immuno-affinity liquid chromatography–tandem mass spectrometry (ILM) assay that quantifies Mycobacterium tuberculosis CFP10 peptides and HIV-1 p24 in serum, showing high sensitivity and specificity in both adults and children. This method allows early detection of HIV and TB and monitors treatment responses effectively. The second approach involves an automated peptidomics pipeline that rapidly identifies mycobacterial species and subspecies from early growth cultures, including drug-resistant strains, significantly reducing diagnosis time compared to current clinical practices. These methods present significant advancements for managing the mycobacterial diseases.<blockquote>Presenter: PhD. Tony Hu (Center for Cellular &amp; Molecular Diagnostics, Tulane Cancer Center Translational Oncology Research Program Leader)Presenter:&nbsp;Aaron Scott (Infection Medicine (BMC) – Faculty of Medicine, Lund University)</blockquote>

Evosep: Tracking early signs of infectious disease with Evosep One to improve patient outcomes

Tracking early signs of infectious disease with Evosep One to improve patient outcomes

Learn how Evosep One’s robustness, sensitivity, and efficiency are helping laboratories and clinicians stay ahead of disease progression.

Tracking early signs of infectious disease with Evosep One

Clinical blood analysis is crucial for monitoring health and diagnosing diseases, with blood-based biomarkers playing a key role.However, current detection methods often lack sufficient coverage. Mass spectrometry-based proteomics provides a robust solution for in-depth plasma proteome analysis, but precise sample preparation is essential.This webinar will guide you on maximizing plasma proteome coverage and optimizing sample preparation, whether using plasma or serum. Dr. König and Dr. Wendt will discuss developing an optimized, automated workflow for comprehensive plasma biomarker discovery, showcasing results from two complementary techniques: LC-MS and Olink.<h4>Attend this webinar to:</h4><ul><li>Discover how to efficiently prepare blood-derived samples, including plasma and serum</li><li>Explore how to implement a fully automatable, reproducible and standardized plasma workflow with scalable throughput</li><li>Achieve comprehensive plasma proteome characterization, addressing the dynamic range challenge for deeper insights</li><li>Learn to apply LC-MS-based proteomics combined with affinity-based methods to discover novel disease biomarkers</li></ul><blockquote>Presenter: Dr. Ann-Christine König (Proteomics Core Facility Manager, Helmholtz Zentrum Munich) Presenter:&nbsp;Dr. Fabian Wendt (Product and Application Manager, Mass Spectrometry, Tecan)</blockquote>

Technology Networks: Unlocking the Plasma Proteome: Advanced Profiling and Biomarker Discovery

Unlocking the Plasma Proteome: Advanced Profiling and Biomarker Discovery

Maximizing plasma proteome coverage and optimizing sample preparation, whether using plasma or serum.

Unlocking the Plasma Proteome: Advanced Profiling

USP &lt;621&gt; is a crucial chapter in the USP guidelines, affecting around 5000 monographs. It emphasizes the importance of establishing meaningful system suitability criteria for chromatography procedures to ensure valid, compendial, and regulatory compliance. The latest version of USP &lt;621&gt;, effective from December 2022, provides detailed guidance on method adjustments and transfers, facilitating the modernization of existing USP monograph methods. The revisions also expand the scope for modifying gradient method parameters and allow the transition from totally porous silica-based analytical columns to superficially porous particle-based columns to better meet industry needs.<h5>Key Learnings:</h5><ul><li>Strategies for optimizing chromatographic performance using right HPLC system</li><li>Understand the latest updates in USP &lt;621&gt; and how they impact chromatographic practices</li><li>Techniques for enhancing laboratory productivity and reducing operational costs</li><li>Strategies to modernize chromatographic conditions with case-studies</li><li>Revised System suitability requirements and allowable chromatographic system adjustments</li><li>and more</li></ul><blockquote>Presenter: Dr. Manoj Kumar Singh (Senior Vice President, Microlabs Analytical Research Lab)Dr. Manoj K Singh hold over 30 yrs of professional experience of working with global pharma companies leading their Analytical labs to support different pharma work-flows.He holds a Ph.D. degree (Chemistry).He started his professional career in 1993 with Wockhardt and spent significant tenure at major pharma R&amp;Ds like Lupin, Novartis, Orchid, Dabur &amp; Sentiss Pharma.Currently he is associated with MicroLabs Bangalore as Senior Vice President (ADL) leading team of Analytical Chemistry scientists to support formulation development, Validations and method transfer.Presenter: Dr. Kuldeep Sharma (Sales Development Manager - Pharma, Agilent Technologies)Kuldeep Sharma has over 19 years of experience in area/s of Bioanalytical research, Drug metabolism &amp; pharmacokinetics and Pharma regulatory compliance. He has accomplished PhD in Biotechnology. His professional journey covers over 12 years in Analytical research (associated with R&amp;D’s of Zydus Cadila, Lambda, Pliva and Jubilant Biosys) and last 7 years in analytical instruments sales, marketing &amp; business development. With over 21 research articles published in international Journals, He presents convention of continues learning.</blockquote>

Agilent: Enhance Lab Productivity While Meeting Regulatory Requirements: USP 621 Decoded

USP 621 Decoded

Enhance Lab Productivity While Meeting Regulatory Requirements

This dynamic symposium will include six 15-minute lightening talks featuring leading academics, industry professionals, and regulatory experts in the field of polymer research and characterization.During the event our speakers will provide cutting-edge insights into the latest developments in polymer characterization and the application of light scattering techniques. In addition to the lecture portion, we are delighted to include two live round table sessions as our speakers engage in lively discussions around topics raised by attendees and those top-of-mind in the industry. Don't miss this opportunity to connect and learn from these leading experts.<h4>Key Learnings:</h4><ul><li>Advances in light scattering and differential viscosity techniques for polymers and innovative applications</li><li>Polymers for industrial applications</li><li>Comparative analysis of complementary techniques</li><li>How to maintain quality data at high-use facilities</li><li>Regulatory considerations in the realm of polymers and light scattering</li></ul><h4>Agenda:</h4><ul><li>10:30 AM EDT - Welcome &amp; Meeting Overview, Introductions of Speakers</li><li>10:35 AM EDT - Prof. Stepan Podzimek, University of Pardubice and SYNPO "Wyatt Technology Advanced SEC/GPC Detectors Over the Past Four Decades"</li><li>10:50 AM EDT - Dr. Phil Griffin, University of Chicago (talk to be announced)</li><li>11:05 AM EDT - Prof. Timothy Lodge, University of Minnesota (talk to be announced)</li><li>11:30 AM EDT - Dr. Peng Zhang, USP "USP's Characterization of LG polymers Utilizing GPC-Multi-Angle Light Scattering and Refractive Index Techniques"</li><li>11:45 AM EDT - Roundtable 1 (all speakers)</li><li>12:00 PM EDT - Prof. Matthew Golder, University of Washington "Analyzing Cyclic Polymer Architectures Synthesized via Ruthenim Catalysis Using GPC-MALS-IV-RI"</li><li>12:15 PM EDT - Dr. Wei Gao, The Dow Chemical Company "Polymers for Industrial Applications"</li><li>12:30 PM EDT - Roundtable 2 (all speakers)</li><li>12:50 PM EDT - Wrap up</li></ul><blockquote>Presenter:&nbsp;Dr. Wei Gao (The Dow Chemical Company)&nbsp;Dr. Gao is a R&amp;D and TS&amp;D Fellow in Analytical Science, Core R&amp;D of Dow. She has made significant contributions on polymer and particle characterization using separation-based analytical techniques, sustainable polymeric product development, and has received numerous awards, including the US EPA Presidential Green Chemistry Challenge Awards and R&amp;D 100 awards. Gao is an active member of the American Chemical Society and leads advanced biodegradation platform development at Dow.Presenter:&nbsp;Prof. Stepan Podzimek (University of Pardubice and SYNPO)Prof. Podzimek holds a professorial position at the University of Pardubice and is a head of the Analytical Chemistry department at the Czech R&amp;D company SYNPO. He is also a scientific consultant for Wyatt technology focusing on applications of multi-angle light scattering for the characterization of all types of synthetic and natural polymers. He has authored over 70 scientific papers and the author of a book entitled “Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation”.Presenter:&nbsp;Prof. Timothy Lodge (University of Minnesota)Prof. Lodge is a Regents Professor and the Prager Chair in Macromolecular Science at the University of Minnesota. He is renowned for his research on polymer systems, particularly in the areas of polymer characterization and nanostructures. Lodge has received numerous prestigious awards, including the American Chemical Society's Award in Polymer Chemistry, and is a Fellow of the American Academy of Arts and Sciences and a member of the National Academy of Engineering.Presenter:&nbsp;Prof. Matthew Golder (University of Washington)Matt received his BS in Chemistry from the University of Rochester in 2010 working with Prof. Patrick Holland. Following graduation, he was a graduate student in the lab of Prof. Ramesh Jasti at Boston University/University of Oregon where he earned his PhD in 2015. Matt was then an NIH Postdoctoral Fellow in Prof. Jeremiah Johnson’s laboratory at MIT. He began his independent career in the Department of Chemistry at the University of Washington in 2019. His group works at the interface of physical organic and polymer chemistries to explore novel structural motifs for solving broad challenges across materials science.Presenter:&nbsp;Prof. Phil Griffin (University of Chicago)Dr .Griffin is an Engineering and Design, Sr. Specialist and Director of the Soft Matter Laboratory at the Pritzker School of Molecular Engineering at the University of Chicago. He supports multiple groups across the university as well as external universities and companies in their investigations into the design and development of advanced materials. He also instructs a graduate-level laboratory course focused on soft materials and regularly guest lectures in other Pritzker School of Molecular Engineering courses.&nbsp;Presenter: Dr. Peng Zhang (USP)Dr. Peng Zhang is a Principal Scientist at the United States Pharmacopeia (USP) in Rockville, MD. In his current role, he is primarily responsible for standard setting activities related to the development and update of high-priority excipient quality standards, including those for lactide (or lactic acid) and glycolide (or glycolic acid) (LG) polymers and phospholipids, under the Excipient Expert Committees. Before joined USP, he worked as an analytical scientist in the pharmaceutical industry for 10 years. He holds a Ph.D. degree in Analytical Chemistry from Vanderbilt University.</blockquote>

Waters Corporation: Light Scattering Symposium and Roundtable on Polymer Development and Analysis

Light Scattering Symposium and Roundtable on Polymer Development and Analysis

Cutting-edge insights into the latest developments in polymer characterization and the application of light scattering techniques.

Light Scattering Symposium and Roundtable on Polymer

This webinar is the first part in a three-part series called "A Systematic UHPLC / HPLC Method Development Strategy with Complementary Stationary Phases to Maximize Selectivity and Resolution." Each part is about 20min long.In this seminar, we review the importance of chromatographic selectivity in RPLC from a theoretical and practical perspective and how this relates to analyte resolution for method development. An overview of the method development workflow is discussed.<blockquote>Presenter: Gemma Lo (Portfolio Manager Avantor)</blockquote>

MAC-MOD ANALYTICAL: Understanding Chromatographic Selectivity

Understanding Chromatographic Selectivity

We review the importance of chromatographic selectivity in RPLC from a theoretical and practical perspective and how this relates to analyte resolution.

This session will provide a comprehensive introduction to the user interface, ensuring you can navigate with ease. Discover the various acquisition modes available and learn how to select the best one for your specific application.Additionally, we will explore the innovative Intelligent Reflex feature, highlighting its benefits in boosting your productivity.Whether you’re new to MassHunter or looking to refine your skills, this webinar is designed to enhance your understanding and efficiency.<blockquote>Presenter: Christian Hegmanns (Application Specialist LC/MS, Agilent Technologies, Waldbronn, Germany)Christian Hegmanns is a state-certified food chemist and works at Agilent as an LC/MS application specialist.With over 10 years of experience in the field of instrumental analysis, Christian develops customer-oriented solutions for a variety of applications with a focus on food analysis.</blockquote>

Agilent Technologies: MassHunter Masterclass Months

MassHunter Acquisition Q-TOF Part I – Basic Overview

Join us for an insightful webinar where we delve into the essentials of MassHunter Acquisition Q-TOF. 