Ultrashort chain per- and polyfluoroalkyl substances (USC-PFAS),defined as those with three or fewer carbon atoms, are characterisedby their significant mobility within the environment, high water solubility,and low affinity for organic matter. These properties contributeto their widespread presence in environmental waters and wastewaters,as well as drinking waters. The environmental concerns associatedwith USC-PFAS include their persistence, potential for groundwatercontamination, and challenges in remediation due to their resistance to conventional treatment methods.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_2_093d51b263.jpg" alt="EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources: Figure 1: Illustrative picture" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_2_093d51b263.jpg 245w," sizes="100vw" width="602" height="247"><h4>Occurence, uses and sources</h4>Ultrashort chain PFAS have been poorly characterised compared with their longer chain counterparts, and understanding of their intended and unintended transformation processes is limited. Sources of USCPFAS include degradation of precursor compounds, atmospheric degradation of hydrofluorocarbons and hydrochlorofluorocarbons used as refrigerants, and intentional direct use in products like batteries. Crucially, TFA, a major ultrashort-chain PFAS, is also formed through the environmental breakdown of fluorinated pesticides and pharmaceuticals. They are also byproducts from historical electrochemical fluorination (ECF) manufacture of longer chain PFAS. USC-PFAS are also found in urban and industrial waste, as well as aqueous film-forming foams (AFFFs) used in firefighting.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_5698c634d6.jpg" alt="EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources: Figure 2: PFSA and PFCA categorization based on carbon chain length – ultrashort-chain, short-chain and long-chain." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_5698c634d6.jpg 245w," sizes="100vw" width="1496" height="629"><h4>Fate and transport</h4>Carbon chain length plays a key role in determining the physicochemical characteristics of different PFAS chemicals. Long chain PFAS for instance are more hydrophobic, a property that has been leveraged by removal techniques using carbonaceous materials like granular activated carbon (GAC). By contrast, short and ultrashort chain PFAS are hydrophilic, with characteristically low pKa values (i.e. more acidic), high water solubilities and decreased log Koc values (i.e. less adsorptive to organic carbon). These attributes mean that USC-PFAS dissociate freely in aqueous environments and are less prone to sorption onto natural solids. The outcome is that USCPFAS are highly mobile in the aquatic environment and can travel far from contamination source areas.<h4>The global regulatory landscape</h4>Awareness about the persistence and bioaccumulative nature of C8-C14 PFAS began to attract widespread concern near the turn of the millennium, and inclusion of PFOS, PFOA and PFHxS on the persistent organic pollutants (POPs) list of the Stockholm Convention soon ensued. Consequentially, manufacturers began adopting short and ultrashort chain alternatives.However, with the change from C8 to C4 and shorter fluorinated carbon chains came the sacrifice to lower technical performance, and greater quantities were needed for equivalent performance. Hence, while it is generally accepted that short and ultrashort chain PFAS have lower toxicological profiles, environmental risks are not necessarily obviated since USC-PFAS presence, and continuous, cumulative, release suggests that adverse risks may lie ahead.Global regulations of USC-PFAS vary by region and are often included under broader PFAS regulations. For example, the European Union has specific guidelines and limits for the total concentration of PFAS compounds in drinking water (as set out in EU Directive 2020/2184). The EU's Drinking Water Directive established a limit of 0.5 μg/L for 'PFAS Total,' although ongoing discussions concern the specific methodology for incorporating trifluoroacetic acid (TFA). Furthermore, TFA was recently added to the priority list for the initial proposed sum of 24 PFAS in surface water. <a target="_blank" rel="noopener noreferrer" href="https://www.consilium.europa.eu/en/press/press-releases/2025/09/23/water-pollution-council-and-parliament-reach-provisional-deal-to-update-priority-substances-in-surface-and-ground-waters/">LINK</a><h4>PFAS sampling containers</h4>Only tested and verified sampling containers are recommended to ensure reliable results in PFAS analysis; these containers are available upon request from ALS laboratories.<h4>Targeted analytical services are becoming more accessible</h4>Testing for USC-PFAS is challenging due to several analytical factors. Background contamination and matrix noise can interfere with the detection of these compounds, particularly trifluoroacetic acid (TFA). Furthermore, sample preparation presents a significant challenge because of the constant risk of cross-contamination. To mitigate this risk, the sample preparation procedure was specifically designed to be as simple and straightforward as possible.Other USC-PFAS compounds generally have lower ambient background concentrations, which makes achieving lower Limits of Reporting (LOR) more readily achievable. ALS has successfully developed a testing method that includes USC-PFAS compounds in environmental water matrices, achieving a total LOR of 50 ng/L for TFA and even lower LORs for other USC compounds. The full list of analyzed USC-PFAS is summarized in table 1:<h5>USC-PFAS Compounds / CAS No.</h5><ul><li>Trifluoroacetic acid (TFA), Perfluoroethanoic acid (PFEtA): 76-05-1</li><li>Trifluoromethane sulfonic acid (TFMS), Perfluoromethane sulfonic acid (PFMeS): 1493-13-6</li><li>Perfluoroethane sulfonic acid (PFEtS): 354-88-1</li><li>Perfluoropropanoic acid (PFPrA): 422-64-0</li><li>Perfluoropropane sulfonic acid (PFPrS): 423-41-6</li><li>Trifluoromethane sulfinic acid: 34642-42-7</li><li>Lithium bis(trifluoromethanesulfonyl)imide (LiTFSI): 90076-65-6</li></ul>&nbsp;

EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources

<h4>Why Consider HILIC in Modern Oligonucleotide Analysis?</h4>Oligonucleotides play a central role in gene therapies, molecular diagnostics, and cutting-edge biomedical research. Before these molecules reach application, however, they must be carefully characterized to confirm purity, identity, and performance.Analyzing therapeutic oligonucleotides is far from straightforward. Their polarity, structural diversity, and modifications demand highly selective and MS-compatible separation methods. Although ion-pair reversed-phase LC (IP-RP-LC) remains the established standard for LC-MS workflows, hydrophilic interaction liquid chromatography (HILIC) is rapidly emerging as a powerful complementary technique.When IP-RP approaches reach practical or performance limits, HILIC offers an attractive alternative, delivering strong retention and efficient separation of highly polar oligonucleotides — without relying on ion-pairing reagents.<h4>How Does HILIC Separate Oligonucleotides?</h4>HILIC essentially reverses conventional reversed-phase logic. It combines:<ul><li>A polar stationary phase</li><li>An organic-rich mobile phase</li></ul>In this configuration, the stationary phase retains a thin, water-enriched layer on its surface. Polar analytes — such as oligonucleotides — partition into this aqueous layer while the acetonitrile-rich mobile phase passes through.This mechanism allows:<ul><li>Retention of short or highly polar oligonucleotides</li><li>Separation of modified sequences</li><li>LC-MS compatibility without ion-pairing reagents</li></ul>The key to performance lies in carefully tuning the parameters that govern HILIC selectivity and retention.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Key_Parameter_of_HILIC_Mode_fb364c9a8b.webp" alt="KNAUER: Figure 1 - Key Parameter of HILIC Mode." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Key_Parameter_of_HILIC_Mode_fb364c9a8b.webp 245w," sizes="100vw" width="945" height="433"><h4>What Controls Retention in HILIC?</h4>Several interaction mechanisms contribute to analyte behavior in HILIC:<h5>Hydrophilic Partitioning – The Primary Mechanism</h5>Polar molecules distribute into the thin water layer formed on the stationary phase surface. The greater the polarity of the analyte, the stronger its affinity for this layer, leading to increased retention.<h5>Hydrogen Bonding – Fine-Tuning Effects</h5>Functional groups such as –OH, –NH₂, and –COOH can form hydrogen bonds with the stationary phase, subtly influencing retention time and selectivity.<h5>Electrostatic Contributions – The Role of Charge</h5>Some HILIC materials (e.g., bare silica) exhibit surface charges. Depending on mobile phase pH and buffer composition, electrostatic interactions can either enhance or suppress analyte retention.<h5>Dipole and Ion–Dipole Interactions – Added Selectivity</h5>Highly polar or zwitterionic compounds — including phosphorylated species and sugars — may engage in dipole–dipole or ion–dipole interactions, further refining separation behavior.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hydrophilic_Partitioning_on_Water_enriched_Layer_41ab9ac902.webp" alt="KNAUER: Figure 2 - Hydrophilic Partitioning on Water-enriched Layer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hydrophilic_Partitioning_on_Water_enriched_Layer_41ab9ac902.webp 245w," sizes="100vw" width="945" height="409"><h4>How to Develop a HILIC Method for Oligonucleotides</h4>Like any chromatographic method, successful HILIC development follows a structured approach:<h5>Step 1: Define the Analytes</h5>Understand oligonucleotide length, polarity, charge, and modifications.<h5>Step 2: Select a Suitable Column</h5>Choose a polar stationary phase (e.g., amide or zwitterionic) appropriate for the oligonucleotide’s chemical properties.<h5>Step 3: Optimize the Mobile Phase</h5><ul><li>Start with 70–95% acetonitrile</li><li>Use a volatile buffer (e.g., ammonium formate or ammonium acetate, typically 5–20 mM)</li><li>Adjust pH (commonly between 6–8, with testing up to pH 9 if column stability allows)</li></ul>Small pH shifts can significantly alter selectivity and MS response.<h5>Step 4: Design the Gradient</h5>Employ a gradient that increases water content to modulate retention and separate oligonucleotides by size or chemical modification.<h5>Step 5: Match Injection Conditions</h5>Samples should be injected in a solvent similar to the starting mobile phase (high organic content) to prevent peak distortion.<h5>Step 6: Evaluate Performance</h5>Assess:<ul><li>Resolution</li><li>Retention time stability</li><li>Peak shape</li><li>Reproducibility</li></ul>Balancing separation performance with MS ionization efficiency is essential.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_HILIC_Method_Step_Procedure_for_Oligos_f3b9ea6b08.webp" alt="KNAUER: Table 1 - HILIC Method Step Procedure for Oligos." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_HILIC_Method_Step_Procedure_for_Oligos_f3b9ea6b08.webp 245w," sizes="100vw" width="945" height="357"><h4>Practical Tips for High-Performance HILIC</h4><h5>Maintain a Stable Water Layer</h5>A minimum of ~5% water is necessary at the start of the run. Too little water can eliminate retention entirely. Adjust water content carefully to fine-tune retention.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Water_Adjustment_Principle_for_HILIC_c0d8616f20.webp" alt="KNAUER: Figure 3 - Water Adjustment Principle for HILIC." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Water_Adjustment_Principle_for_HILIC_c0d8616f20.webp 245w," sizes="100vw" width="951" height="374"><h5>Use High Organic Content</h5>HILIC typically operates best with 70–95% acetonitrile. Polar analytes are retained more effectively under these conditions.<h5>Include a Volatile Buffer</h5>Use low concentrations (5–20 mM) of ammonium formate or acetate to:<ul><li>Stabilize pH</li><li>Reduce peak tailing</li><li>Maintain MS compatibility</li></ul><h5>Control Injection Solvent</h5>Avoid high-water sample solvents. They disrupt the partitioning mechanism and distort peaks.<h5>Allow Proper Column Equilibration</h5>HILIC columns require longer equilibration times than reversed-phase columns — typically 10–20 column volumes after major gradient or solvent changes.<h5>Consider Temperature</h5>Operating at 30–50 °C can:<ul><li>Improve peak shape</li><li>Reduce mobile phase viscosity</li><li>Stabilize retention behavior</li></ul><h4>From Alternative to Essential? Final Perspective</h4>HILIC-MS is steadily advancing as a strong, ion-pair-free option for oligonucleotide analysis. While IP-RP-LC still dominates in sensitivity for many applications, HILIC is closing the gap and offering valuable flexibility — particularly for highly polar or challenging sequences.Although HILIC follows different chromatographic principles than reversed-phase separations, once optimized, it can deliver robust and highly selective results.Rather than being a niche method, HILIC is becoming a strategic tool in modern oligonucleotide workflows — especially when MS compatibility and method versatility are priorities.If you are developing or refining an oligonucleotide LC-MS method, HILIC may be worth integrating into your analytical toolbox.<blockquote>For additional information or discussion, feel free to contact: <a>sales@knauer.net<svg><use></use></svg></a>For direct scientific inquiries, reach out to the author at: <a>kindler@knauer.net<svg><use></use></svg></a></blockquote>Stay tuned for more insights in the “Oligos Made Easy” series.

Oligos Made Easy - Part 4: HILIC

News from LabRulezLCMS Library - Week 09, 2026

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For more than 40 years, ALS has provided testing and technical solutions to clients all over the world in a wide range of industries. We have built our global reputation by embracing the latest technologies and innovative methodologies to deliver high quality testing services to clients. ALS is able to leverage a multifaceted international team while remaining agile enough to provide local expertise and personalized solutions to every one of our clients.

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LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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<h4>Live Interactive Web Series</h4>
We’ll work through a topic of the week (roughly 60 minutes) and discuss any questions that come up along the way. During these sessions, we’ll talk about how to care for your SEC-MALS or FFF-MALS system, help you brush up on your ASTRA and DYNAMICS data processing, discuss measuring dn/dc values and why dn/dc is important, and see how stable RI baselines are achievable.
Working with polydisperse samples? We’ll see how to leverage ASTRA’s distribution analysis and results fitting features. Special topics on error analysis, batch MALS work, and more.
The live interactive web series are 100% live sessions, with anonymous Q&amp;A welcome during and after the discussion. Free for all to attend.
<ul>
<li>Part 1 of 6: Why we love Multi-Angle Light Scattering! How MALS works and what it measures. Modes of operation and when to use them*
<ul>
<li>MALS theory and operating principles</li>
<li>Batch MALS vs. flow mode MALS: when and why</li>
<li>What MALS can and cannot measure directly</li>
</ul>
</li>
<li>Part 2 of 6: Getting to know your MALS hardware, including ‘proper care &amp; feeding’ of the system, practical guides for optimizing data quality, and how to know when your DAWN hardware needs special attention*
<ul>
<li>Understanding the MALS hardware internal and external components</li>
<li>Diagnosing hardware issues</li>
<li>How to help your MALS operate at its best</li>
</ul>
</li>
<li>Part 3 of 6: Let it flow - Leveraging MALS with Size Exclusion Chromatography SEC / Gel Permeation Chromatography GPC. Considerations for successful SEC-MALS / GPC-MALS operation. How to process ASTRA data on and after day 1*
<ul>
<li>Practical considerations for MALS in SEC/GPC mode</li>
<li>Understanding system settings &amp; coefficients such as inter-detector band broadening and normalization</li>
<li>Squeezing the most information from your SEC-MALS data</li>
</ul>
</li>
<li>Part 4 of 6: Advanced topics in ASTRA data analysis - uncertainty calculations, MW averages, mass recovery, distribution analysis, results fitting, and data exporting*
<ul>
<li>Understanding the ways ASTRA tracks and calculates system errors</li>
<li>Leveraging peak integration to understand what’s in your sample</li>
<li>When and why to use the various MW averages: Mn, Mw, Mz, Mp, Mavg, Mv, etc.</li>
<li>Efficient and easy ways to export exactly the data and results you want, right out of ASTRA</li>
</ul>
</li>
<li>Part 5 of 6: All about dn/dc values and concentration detectors (UV and RI) for MALS*
<ul>
<li>Understanding dn/dc, what it is, and how it is used with MALS</li>
<li>Measuring / determining dn/dc for your samples</li>
<li>UV detectors and their role in MALS</li>
<li>Mass recovery in ASTRA</li>
</ul>
</li>
<li>Part 6 of 6: MALS flow cell care: diagnosing and cleaning your flow cell*
<ul>
<li>How to avoid the need to clean your MALS flow cell in the first place</li>
<li>How to diagnose a dirty MALS flow cell</li>
<li>How to take apart and clean your MALS flow cell</li>
</ul>
</li>
</ul>
*It is recommended but not required that you attend all the sessions in this series.
<blockquote>
Presenter: Dr. Andy Meyer
</blockquote>
<blockquote>
Dr. Andy Meyer joined Wyatt Technology in 2001, serving for several years as an Applications Scientist, then as Dean of Wyatt&#39;s &quot;Light Scattering University&quot; and as Director of Customer Service &amp; Support. After managing multiple regions in North America, Dr. Meyer is now a Senior Application Scientist for Wyatt. As a Wyatt customer, he completed his Ph.D. in Applied Science – Polymer Chemistry from the College of William and Mary in Virginia.
</blockquote>

Waters Corporation: All about dn/dc values and concentration detectors (UV and RI) for MALS

All about dn/dc values and concentration detectors (UV and RI) for MALS

All about dn/dc values and concentration detectors for MALS

Gel permeation chromatography (GPC), also known as size exclusion chromatography (SEC), is a powerful analytical technique for macromolecule-related research and applications. GPC/SEC plays a pivotal role in the chemical industry, offering insights into the molecular weight distribution of polymers.
In this webinar, our expert speakers will outline basic theory and the principle of method development, and will provide a practical guide on column selection. Furthermore, the presentation will also discuss the limitations of GPC/SEC and the factors contributing to abnormal size exclusion behaviors using real industrial application examples.
Join us as we navigate the world of GPC/SEC, shedding light on its principles, practices, and its indispensable role in polymer research in industry.
Key learning objectives
<ul>
<li>Learn about basic theory of GPC/SEC</li>
<li>Explore the principle of GPC method development</li>
<li>Discover GPC/SEC column selection</li>
<li>Consider the limitations of GPC/SEC</li>
</ul>
Who should attend?
<ul>
<li>Polymer scientists</li>
<li>Food scientists</li>
<li>Environmental scientists</li>
<li>Pharma/biopharma researchers</li>
<li>Principle investigators</li>
<li>Laboratory managers and technicians/technologists</li>
</ul>
<h4>View other webinars in this series here:</h4>
<ul>
<li>Part 2: <a href="https://lcms.labrulez.com/webinar/3691">Detection in Gel Permeation Chromatography /Size Exclusion Chromatography</a>
</li>
<li>Part 3: <a href="https://lcms.labrulez.com/webinar/3692">Characterization of Macromolecules by SEC-Light Scattering</a>
</li>
<li>Part 4: <a href="https://lcms.labrulez.com/webinar/3693">Concepts, Challenges and Applications for 2D-LC of Polymers</a>
</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Adrian Williams (Product Marketing, GPC/SEC Consumables, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Adrian Williams holds a BSc (Hons) degree in Applied Chemistry from Wolverhampton University, UK. He currently works in Product Marketing for GPC/SEC consumables at Agilent Technologies. Prior to this, he worked at Polymer Standards Service (PSS) GmbH in Mainz, Germany (now a part of Agilent Technologies) for 12 years. He also worked at Polymer Laboratories Ltd. (later Varian and Agilent Technologies) for 28 years, specializing in polymer characterization using GPC/SEC.
</blockquote>
<blockquote>
Presenter: Dr. Wei Gao (R&amp;D/TS&amp;D Fellow, Dow Inc.)
</blockquote>
<blockquote>
Dr. Wei Gao is an R&amp;D/TS&amp;D Fellow at the Dow Chemical Company. She received her PhD in Polymer Chemistry and Physics from Peking University. Her research interests are polymer and particle characterization, the synthesis-structure-property relationship of polymeric and colloidal systems, and sustainable polymers. She has organized multiple symposia at ACS national meetings and co-edited an ACS Symposium series book. Wei has 50+ peer review publications and 13 granted patents with Dow.
</blockquote>
<blockquote>
Presenter: Jemima Arnold (Editorial Assistant, SelectScience)
</blockquote>
<blockquote>
Jemima studied Cellular and Molecular Medicine at the University of Bristol, UK, and as a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

SelectScience: Unraveling the Intricacies of Size Exclusion Chromatography – Strength and Limitations

Unraveling the Intricacies of Size Exclusion Chromatography – Strength and Limitations

Join us as we navigate the world of GPC/SEC, shedding light on its principles, practices, and its indispensable role in polymer research in industry.

Unraveling the Intricacies of Size Exclusion Chromatography

In this live webinar, we will introduce the brand new Thermo Scientific™ Hypersil GOLD™ Peptide LC Column, designed for all peptide separation, including excellent retention of hydrophilic peptides and separation of deamidated species in a single run. This column is ideal for the development and manufacturing of biopharmaceuticals, achieving minimal RSD% and impeccable lot-to-lot consistency.
Following a product presentation of this advanced technology, you will get a chance to hear testimonies and results from our customers and early-adopters in the field. Finally, our experts will proceed to dive deeper into the related applications, such as peptide mapping and monitoring as part of the Multi-Attribute Method (MAM), to provide you with the solutions to resolve all your peptide challenges. We hope to see you there! 
Learning Points:
<ul>
<li>Introduction to the new Hypersil GOLD Peptide LC Column</li>
<li>Hear from early adopters about their use of this advanced technology</li>
<li>Dive deeper into the related applications to resolve all peptide challenges</li>
</ul>
Who should attend?
<ul>
<li>Biopharmaceutical research scientists</li>
<li>Analytical scientists from biopharma companies working on or interested in peptide mapping and monitoring</li>
<li>PhD students and scientists looking to advance their knowledge of peptide mapping and monitoring</li>
</ul>
Short notice:
Can&#39;t attend the live broadcast? No problem! Register anyway and you will automatically receive the link to the webinar recording. 
<blockquote>
Presenter: Serdar Bilgesoy (Product Manager Premier LC columns, Thermo Fisher Scientific)
</blockquote>
<blockquote>
As the Global Product Manager for LC Columns at Thermo Fisher Scientific, Serdar brings over 15 years of experience in analytical instruments across various roles in product management, sales, marketing, application, and service responsibilities. He has specialized in segments such as Life Sciences, Clinical, Pharma, Biopharma, and Forensics. He is an experienced Application, Sales, and Marketing Executive in HPLC, LC-MS/MS, LC-MS/TOF, and MALDI-TOF instruments, possessing sophisticated marketing knowledge, a vast sales network, technical background, and innovative solutions expertise.
</blockquote>

ThermoFisher Scientific: Resolve all your peptide challenges: deep dive into new separation technology and applications

Resolve all your peptide challenges: deep dive into new separation technology and applications

We will introduce the brand new Thermo Scientific™ Hypersil GOLD™ Peptide LC Column, designed for all peptide separation.

Peptide challenges: new separation technology

Microbes, the oldest and most abundant life forms on Earth, reside within us in staggering numbers, outweighing our own cells. These microscopic marvels not only play a critical role in our health but also hold the key to groundbreaking discoveries, from antibiotics to gene editing.
Here&#39;s an astonishing piece of information: over 90% of Earth&#39;s microbes remain uncharacterized, forming this immense hidden realm called the Dark Microbiome. This unexplored territory brims with immense potential for future advancements in medicine, biotechnology, and drug discovery.
In this insightful webinar, you will discover:
<ul>
<li>The unique role of Mass Spectrometry-based proteomics in unlocking the functional capabilities of microbiomes (aka. Metaproteomics) and its profound impact on understanding health and disease.</li>
<li>How we use the groundbreaking capabilities of the timsTOF Ultra mass spectrometer to reach unprecedented depth, sensitivity, and quantitative analysis of complex microbiome samples.</li>
<li>How we push the boundaries of sensitivity to achieve sub-pg peptide detection, paving the way to discover new biology.</li>
</ul>
<blockquote>
Presenter: David Gómez-Varela, Ph.D. (Systems Biology of Pain, Division of Pharmacology and Toxicology, University of Vienna, Vienna, Austria)
</blockquote>

Bruker: Clinical Proteomics: Ultra-sensitive mass spectrometry sheds light on the microbiome

Ultra-sensitive mass spectrometry sheds light on the microbiome

You will discover the role of Mass Spectrometry-based proteomics and the capabilities of the timsTOF Ultra mass spectrometer.

Ultra-sensitive mass spectrometry and the microbiome

Focus LC: A New Paradigm with Proven Results
Lab sustainability while reducing the time, cost and complexity of HPLC testing. 
Join CEO Greg Ward to learn first-hand how Axcend customers are reducing the time, cost and complexity of their analytical research.
<blockquote>
Presenter: Greg Ward (CEO Axcend)
</blockquote>

Axcend: A New HPLC Paradigm with Proven Results

A New HPLC Paradigm with Proven Results

Lab sustainability while reducing the time, cost and complexity of HPLC testing. 

By attending this eSeminar, you will:
<ul>
<li>Gain practical insights into optimizing sample preparation in alignment with EPA Method 1633’s regulatory requirements by the use of dual-phase SPE cartridges, covering background analysis, MDL studies, and recoveries</li>
<li>Benefit from real-world experiences from Complete Environmental Testing, offering valuable insights on navigating EPA 1633 in an end-to-end workflow, while utilizing dual-phase SPE cartridges to improve laboratory efficiency</li>
<li>Understand key details for successful PFAS analysis</li>
<li>Learn how to optimize analysis for ultra-short chain PFAS compounds</li>
<li>See how to expand the range of PFAS compounds to analyze in a single run</li>
</ul>
<h4>Event Overview</h4>
Separation Science, in collaboration with its sponsors Waters and Restek, is pleased to bring you an upcoming online eSeminar focusing on advances in PFAS analysis. This unique event will bring together key experts in the field and provide an interactive learning environment for scientists working in this industry.
<h4>Presentations</h4>
Efficiency in EPA Method 1633: Validation and Implementation Strategies
by Jeffrey Smith, Director of Operations, Complete Environmental Testing (CET)
Join us for a presentation focused on optimizing EPA Method 1633 in your laboratory highlighting the validation and implementation of dual-phase WAX and GCB cartridges. EPA 1633 establishes a method for the determination of 40 PFAS from challenging matrices such as non-potable waters, soils, biosolids, and tissue. For these matrix types, sample preparation has moved beyond the use of a WAX cartridge alone. GCB has been added to the sample preparation workflow to provide additional sample cleanup. We will discuss the full EPA 1633 workflow, including topics such as background analysis for soils and water, MDLs achieved, observed recovery in authentic samples, and the practical advantages of dual-phase cartridges in reducing manual labor steps compared to loose GCB in EPA 1633.
This presentation is designed for scientists, laboratory managers, and others looking to implement and validate EPA Method 1633 or optimize their sample preparation process in their current workflow. Join us as Complete Environmental Testing shares experiences on working with the complex matrices in EPA 1633 and breaks down advantages in their method workflow with the use of dual-phase SPE cartridges.
Untapped Ultrashort-chain PFAS Analysis Combined with Routine PFAS Analysis in a Single LC-MS/MS Run
by Mike Chang, Market Development Manager, Restek Corporation
Starting from measuring C8 many decades ago, today’s PFAS testing requires a lot more chromatography capabilities when an extended list of PFAS analysis is desired. Chain length ranging from C1 to C18 and a functional group changing from carboxylic acids to ethers, sulfonamides, and more, with multiple oxygens in the structure, can be challenging for a chromatograph all in one run. In this presentation, we will see examples of a combined PFAS panel analyzed in one run with chain length from C1 and above, in addition to AFFF and FTS compounds.
<h4>What you need to know:</h4>
Date: 16 April, 2024
Start times:
<ul>
<li>Broadcast 1: 9 am BST (London) / 10 am CEST (Paris/Berlin) / 1:30 pm IST (New Delhi) / 4 pm SGT (Singapore)
</li>
<li>Broadcast 2: 10 am PDT (Los Angeles) / 12 pm CDT (Chicago) / 1 pm EDT (New York)
</li>
</ul>
Duration: Approximately 90 minutes
<blockquote>
Presenter: Jeffrey Smith (Director of Operations, Complete Environmental Testing (CET))
</blockquote>
<blockquote>
Jeffrey Smith holds a BS in Chemistry from Quinnipiac University. He has been working in environmental analytical chemistry for 18 years, with experience in several disciplines, including GC/MS and LC-MS/MS. Jeffrey now serves as the director of operations at Complete Environmental Testing. For the past few years, his focus as director has been building the PFAS department at CET from the ground up, including method development and researching LC/MS/MS instrumentation for the laboratory. Jeffrey serves on several PFAS work groups and is an active member of the Reasonable Confidence QA/QC Protocols work group for the state of Connecticut.
</blockquote>
<blockquote>
Presenter: Mike Chang (Market Development Manager, Restek Corporation)
</blockquote>
<blockquote>
With an analytical chemistry background and 15+ years of experience in the chromatography industry, from instrumentation to applications, Mike is the key liaison to testing agencies and industries, bringing better ways of measuring contaminants in the environment and food. He has been chairing PFAS sessions and presenting PFAS topics at various conferences.
</blockquote>

Advances in PFAS Analysis

Understand essential details for successful PFAS analysis and optimize EPA Method 1633 in your lab.

Food quality control is a key step in the manufacturing process of a successful product. It aims at ensuring that the public consume safe food products free from risks associated with contamination or exposure to allergens. It also helps combat fraudulent activities which can undermine the authenticity and economic value of a food product. For all these reasons, developing fast and robust analytical methods for food quality control is important.
MALDI-TOF mass spectrometry is a well-established and widely used technique in various fields. Compared to other analytical techniques, it is particularly appreciated for its speed of analysis, simplicity and robustness. In this webinar, we will present some examples of MALDI-based applications for food quality control aiming at tackling issues of food adulteration and safety, using a small benchtop MALDI-TOF instrument from Shimadzu.
We will host a Q&amp;A session after the presentation to address any questions or comments.
<blockquote>
Presenter: Nazim Boutaghou, Ph.D. (Life Science Product Manager, Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Nazim Boutaghou received a Master’s degree with a dual major in Chemistry and Biology from the University of Bordeaux 1. He joined Dr. Jean Marie Schmitter’s research group at the European Institute of Chemistry and Biology and worked on establishing H/D exchange protocols to study molecular processes taking place in the early onset of the Alzheimer’s disease. After a short break from science to study business management, Nazim joined Dr. Richard Coles’ group at the Louisiana State University in New Orleans (UNO) and advanced his work in the field of Mass Spectrometry, specializing mostly on MALDI-based workflows. He authored and co-authored several publications related to development of novel MALDI matrices, and various proteomics topics. He joined Shimadzu Scientific Instruments (SSI, American Subsidiary of Shimadzu Corporation) in 2013 as an application scientist and became product manager for the life Science products in 2019. He leads a team of several scientist that support life science products and applications for SSI.
</blockquote>
<blockquote>
Presenter: Simona Salivo (Senior MALDI Applications Specialist, Kratos Analytical)
</blockquote>
<blockquote>
Simona Salivo was born in Reggio Calabria, Italy. She graduated in “Chemistry and Pharmaceutical Technologies” and earned her Ph.D. degree at the Department of Pharmaceutical Science and Health Products of the University of Messina, Italy. She studied under the guidance of the renowned Prof. Luigi Mondello and Prof. Peter Quinto Tranchida and focused during her graduate studies on the development of multidimensional gas chromatography techniques coupled with single quadrupole and high-resolution mass spectrometry for the characterization of lipids in food and biological matrices. Throughout her research, she developed a passion for structural characterisation of lipids which she brought into her current role at Shimadzu. During her Ph.D., she was awarded a 6-month work experience at Shimadzu Corporation in Kyoto, Japan, where she worked on MALDI-TOF MS and MALDI imaging of lipids. This started her journey into the world of MALDI mass spectrometry and the Shimadzu family where she is now a Senior MALDI Applications Specialist at Kratos Analytical in Manchester, UK. In her job, she works with her team to develop numerous MALDI-based applications to showcase the Shimadzu MALDI products, and she works closely with the R&amp;D Department on the development of the Shimadzu MALDI instruments and software, by providing technical feedback and evaluating the products at the different stages of development and manufacturing.
</blockquote>

Shimadzu: Shimadzu MALDI-Based Solutions for Food Quality Control

Shimadzu MALDI-Based Solutions for Food Quality Control

We will present some examples of MALDI-based applications for food quality control aiming at tackling issues of food adulteration and safety.

Audit Trails are a great tool for troubleshooting and understanding what has happened or changed in your chromatography data system (CDS). If you are in a regulated laboratory, you must also perform periodic review of Audit Trails to ensure data integrity and monitor for impact to the quality of your products, especially entries associated with the results generated for batches of product to be released.
In this webinar, you will gain an understanding of how to use the information captured in the various Audit Trails within Empower Software and in the Empower Message Center. While the Empower Message Center is technically not an Audit Trail and provides information about the Empower Software system, it is imperative that you perform a periodic review of this as well because it contains important information such as instrument and processing errors. We will also demonstrate the Empower Result Audit Viewer, a tool that simplifies review by bringing Audit Trail information for a Result Set into one convenient window.
Topics to be covered include:
<ul>
<li>System Audit Trail</li>
<li>Project Audit Trail</li>
<li>Method Audit Trail</li>
<li>Sample Audit Trail</li>
<li>Sample Set Audit Trail</li>
<li>Result Audit Viewer</li>
<li>Message Center</li>
</ul>
Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.
<blockquote>
Presenter: Neil Lander (Principal Product Manager, Informatics, Waters Corporation)
</blockquote>
<blockquote>
Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.
</blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Using and Reviewing Information in Empower Software Audit Trails

Empower Tips: Ask Me Anything About Using and Reviewing Information in Empower Software Audit Trails

Understand how to use the information captured in the various Audit Trails within Empower Software and in the Empower Message Center.

Reviewing Information in Empower Software Audit Trails

<h3>PFAS and us: The science</h3>
Driving change for a healthier world
Webinar series
<h4>Enrich your understanding of PFAS chemicals and their impact</h4>
Stay on the leading edge of all things PFAS with this enlightening monthly webinar series on testing workflows and cutting-edge technology that will demystify these “forever chemicals”.
Each month, in these 30-minute sessions, industry thought leaders unite to share the current state of PFAS testing and the far-reaching impact that PFAS have on human health and the environment. Build on your current knowledge of testing methodologies and research techniques that will help you develop your own innovative strategies for precise identification and quantitation. Make a lasting impact and join us for these conversations.
<h4>This Webinar:</h4>
April 10, 2024 10:00 a.m. PT/ 1:00 p.m. ET - Examining the exogenous: PFAS and beyond
<blockquote>
Presenter: Andrew Patterson (Vice President, Innovation and Development, Eurofins Environmental Testing America)
</blockquote>
<h4>Other Webinars from the Series:</h4>
May 8, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3866">Rapid PFAS testing with no compromises: direct-inject analysis</a>
<blockquote>
Presenter: Jamie Fox (PFAS Technical Program Manager, Enthalpy Analytical)
</blockquote>
June 12, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3867">Assessment of advanced analytical instruments to reach required limits of quantitation for PFAS in foods</a>
<blockquote>
Presenter: Susan Genualdi, PhD (Research Chemist, FDA, Center for Food Safety and Applied Nutrition, Office of Regulatory Science)
</blockquote>
July 17, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3868">Detecting and identifying known and novel per/polyfluoroalkyl substances (PFAS) in a marine apex predator</a>
<blockquote>
Presenter: Carrie McDonough (Assistant Professor, Chemistry, Carnegie Mellon University)
</blockquote>
August 14, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3869">Whole blood microsampling methods for PFAS exposure biomonitoring</a>
<blockquote>
Presenter: Andrew J. Morris, PhD (Professor, University of Arkansas for Medical Sciences, Research Career Scientist, Central Arkansas Veterans Affairs Healthcare System)
</blockquote>
September 11, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3870">Analysis of ultra short-chain PFCAs to better understand environmental sources and fate</a>
<blockquote>
Presenter: Cora J. Young (Associate Professor, Department of Chemistry, York University)
</blockquote>

Examining the exogenous: PFAS and beyond

Stay on the leading edge of all things PFAS with this enlightening monthly webinar series.

<h5>The Problem</h5>Lab automation saves time, reduces errors, and improves efficiency, but the biggest challenge is mastering complex software. Flexible hardware needs equally flexible software.<h5>The Solution</h5>Big Kahuna and Junior are automation platforms powered by LEA (Laboratory Execution &amp; Analysis) software, allowing users to design, run, and analyze workflows. With Python scripting and API access, users can customize and automate complex lab processes with ease.<h5>The Proof</h5>In this webinar, you'll see how LEA software simplifies automation with Big Kahuna and Junior. We'll show hardware setups, script-based customization, and how to build AI/ML-driven closed-loop automation for your chemistry workflows.<blockquote>Presenter: Del Ray Jackson (Market Manager, Buffer Exchange &amp; Automation, Unchained Labs)Presenter:&nbsp;Andrew Stephenson (Field Automation Scientist / Workflow Architect, Unchained Labs)</blockquote>

Unchained Labs: Score easy chemistry automation driven by streamlined software with Junior & Big Kahuna

Score easy chemistry automation driven by streamlined software with Junior & Big Kahuna

See how LEA software powers automation with Big Kahuna & Junior—making complex chemistry workflows easier, faster, and accessible to all lab users.