Ultrashort chain per- and polyfluoroalkyl substances (USC-PFAS),defined as those with three or fewer carbon atoms, are characterisedby their significant mobility within the environment, high water solubility,and low affinity for organic matter. These properties contributeto their widespread presence in environmental waters and wastewaters,as well as drinking waters. The environmental concerns associatedwith USC-PFAS include their persistence, potential for groundwatercontamination, and challenges in remediation due to their resistance to conventional treatment methods.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_2_093d51b263.jpg" alt="EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources: Figure 1: Illustrative picture" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_2_093d51b263.jpg 245w," sizes="100vw" width="602" height="247"><h4>Occurence, uses and sources</h4>Ultrashort chain PFAS have been poorly characterised compared with their longer chain counterparts, and understanding of their intended and unintended transformation processes is limited. Sources of USCPFAS include degradation of precursor compounds, atmospheric degradation of hydrofluorocarbons and hydrochlorofluorocarbons used as refrigerants, and intentional direct use in products like batteries. Crucially, TFA, a major ultrashort-chain PFAS, is also formed through the environmental breakdown of fluorinated pesticides and pharmaceuticals. They are also byproducts from historical electrochemical fluorination (ECF) manufacture of longer chain PFAS. USC-PFAS are also found in urban and industrial waste, as well as aqueous film-forming foams (AFFFs) used in firefighting.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_5698c634d6.jpg" alt="EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources: Figure 2: PFSA and PFCA categorization based on carbon chain length – ultrashort-chain, short-chain and long-chain." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_5698c634d6.jpg 245w," sizes="100vw" width="1496" height="629"><h4>Fate and transport</h4>Carbon chain length plays a key role in determining the physicochemical characteristics of different PFAS chemicals. Long chain PFAS for instance are more hydrophobic, a property that has been leveraged by removal techniques using carbonaceous materials like granular activated carbon (GAC). By contrast, short and ultrashort chain PFAS are hydrophilic, with characteristically low pKa values (i.e. more acidic), high water solubilities and decreased log Koc values (i.e. less adsorptive to organic carbon). These attributes mean that USC-PFAS dissociate freely in aqueous environments and are less prone to sorption onto natural solids. The outcome is that USCPFAS are highly mobile in the aquatic environment and can travel far from contamination source areas.<h4>The global regulatory landscape</h4>Awareness about the persistence and bioaccumulative nature of C8-C14 PFAS began to attract widespread concern near the turn of the millennium, and inclusion of PFOS, PFOA and PFHxS on the persistent organic pollutants (POPs) list of the Stockholm Convention soon ensued. Consequentially, manufacturers began adopting short and ultrashort chain alternatives.However, with the change from C8 to C4 and shorter fluorinated carbon chains came the sacrifice to lower technical performance, and greater quantities were needed for equivalent performance. Hence, while it is generally accepted that short and ultrashort chain PFAS have lower toxicological profiles, environmental risks are not necessarily obviated since USC-PFAS presence, and continuous, cumulative, release suggests that adverse risks may lie ahead.Global regulations of USC-PFAS vary by region and are often included under broader PFAS regulations. For example, the European Union has specific guidelines and limits for the total concentration of PFAS compounds in drinking water (as set out in EU Directive 2020/2184). The EU's Drinking Water Directive established a limit of 0.5 μg/L for 'PFAS Total,' although ongoing discussions concern the specific methodology for incorporating trifluoroacetic acid (TFA). Furthermore, TFA was recently added to the priority list for the initial proposed sum of 24 PFAS in surface water. <a target="_blank" rel="noopener noreferrer" href="https://www.consilium.europa.eu/en/press/press-releases/2025/09/23/water-pollution-council-and-parliament-reach-provisional-deal-to-update-priority-substances-in-surface-and-ground-waters/">LINK</a><h4>PFAS sampling containers</h4>Only tested and verified sampling containers are recommended to ensure reliable results in PFAS analysis; these containers are available upon request from ALS laboratories.<h4>Targeted analytical services are becoming more accessible</h4>Testing for USC-PFAS is challenging due to several analytical factors. Background contamination and matrix noise can interfere with the detection of these compounds, particularly trifluoroacetic acid (TFA). Furthermore, sample preparation presents a significant challenge because of the constant risk of cross-contamination. To mitigate this risk, the sample preparation procedure was specifically designed to be as simple and straightforward as possible.Other USC-PFAS compounds generally have lower ambient background concentrations, which makes achieving lower Limits of Reporting (LOR) more readily achievable. ALS has successfully developed a testing method that includes USC-PFAS compounds in environmental water matrices, achieving a total LOR of 50 ng/L for TFA and even lower LORs for other USC compounds. The full list of analyzed USC-PFAS is summarized in table 1:<h5>USC-PFAS Compounds / CAS No.</h5><ul><li>Trifluoroacetic acid (TFA), Perfluoroethanoic acid (PFEtA): 76-05-1</li><li>Trifluoromethane sulfonic acid (TFMS), Perfluoromethane sulfonic acid (PFMeS): 1493-13-6</li><li>Perfluoroethane sulfonic acid (PFEtS): 354-88-1</li><li>Perfluoropropanoic acid (PFPrA): 422-64-0</li><li>Perfluoropropane sulfonic acid (PFPrS): 423-41-6</li><li>Trifluoromethane sulfinic acid: 34642-42-7</li><li>Lithium bis(trifluoromethanesulfonyl)imide (LiTFSI): 90076-65-6</li></ul>&nbsp;

EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources

<h4>Why Consider HILIC in Modern Oligonucleotide Analysis?</h4>Oligonucleotides play a central role in gene therapies, molecular diagnostics, and cutting-edge biomedical research. Before these molecules reach application, however, they must be carefully characterized to confirm purity, identity, and performance.Analyzing therapeutic oligonucleotides is far from straightforward. Their polarity, structural diversity, and modifications demand highly selective and MS-compatible separation methods. Although ion-pair reversed-phase LC (IP-RP-LC) remains the established standard for LC-MS workflows, hydrophilic interaction liquid chromatography (HILIC) is rapidly emerging as a powerful complementary technique.When IP-RP approaches reach practical or performance limits, HILIC offers an attractive alternative, delivering strong retention and efficient separation of highly polar oligonucleotides — without relying on ion-pairing reagents.<h4>How Does HILIC Separate Oligonucleotides?</h4>HILIC essentially reverses conventional reversed-phase logic. It combines:<ul><li>A polar stationary phase</li><li>An organic-rich mobile phase</li></ul>In this configuration, the stationary phase retains a thin, water-enriched layer on its surface. Polar analytes — such as oligonucleotides — partition into this aqueous layer while the acetonitrile-rich mobile phase passes through.This mechanism allows:<ul><li>Retention of short or highly polar oligonucleotides</li><li>Separation of modified sequences</li><li>LC-MS compatibility without ion-pairing reagents</li></ul>The key to performance lies in carefully tuning the parameters that govern HILIC selectivity and retention.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Key_Parameter_of_HILIC_Mode_fb364c9a8b.webp" alt="KNAUER: Figure 1 - Key Parameter of HILIC Mode." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Key_Parameter_of_HILIC_Mode_fb364c9a8b.webp 245w," sizes="100vw" width="945" height="433"><h4>What Controls Retention in HILIC?</h4>Several interaction mechanisms contribute to analyte behavior in HILIC:<h5>Hydrophilic Partitioning – The Primary Mechanism</h5>Polar molecules distribute into the thin water layer formed on the stationary phase surface. The greater the polarity of the analyte, the stronger its affinity for this layer, leading to increased retention.<h5>Hydrogen Bonding – Fine-Tuning Effects</h5>Functional groups such as –OH, –NH₂, and –COOH can form hydrogen bonds with the stationary phase, subtly influencing retention time and selectivity.<h5>Electrostatic Contributions – The Role of Charge</h5>Some HILIC materials (e.g., bare silica) exhibit surface charges. Depending on mobile phase pH and buffer composition, electrostatic interactions can either enhance or suppress analyte retention.<h5>Dipole and Ion–Dipole Interactions – Added Selectivity</h5>Highly polar or zwitterionic compounds — including phosphorylated species and sugars — may engage in dipole–dipole or ion–dipole interactions, further refining separation behavior.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hydrophilic_Partitioning_on_Water_enriched_Layer_41ab9ac902.webp" alt="KNAUER: Figure 2 - Hydrophilic Partitioning on Water-enriched Layer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hydrophilic_Partitioning_on_Water_enriched_Layer_41ab9ac902.webp 245w," sizes="100vw" width="945" height="409"><h4>How to Develop a HILIC Method for Oligonucleotides</h4>Like any chromatographic method, successful HILIC development follows a structured approach:<h5>Step 1: Define the Analytes</h5>Understand oligonucleotide length, polarity, charge, and modifications.<h5>Step 2: Select a Suitable Column</h5>Choose a polar stationary phase (e.g., amide or zwitterionic) appropriate for the oligonucleotide’s chemical properties.<h5>Step 3: Optimize the Mobile Phase</h5><ul><li>Start with 70–95% acetonitrile</li><li>Use a volatile buffer (e.g., ammonium formate or ammonium acetate, typically 5–20 mM)</li><li>Adjust pH (commonly between 6–8, with testing up to pH 9 if column stability allows)</li></ul>Small pH shifts can significantly alter selectivity and MS response.<h5>Step 4: Design the Gradient</h5>Employ a gradient that increases water content to modulate retention and separate oligonucleotides by size or chemical modification.<h5>Step 5: Match Injection Conditions</h5>Samples should be injected in a solvent similar to the starting mobile phase (high organic content) to prevent peak distortion.<h5>Step 6: Evaluate Performance</h5>Assess:<ul><li>Resolution</li><li>Retention time stability</li><li>Peak shape</li><li>Reproducibility</li></ul>Balancing separation performance with MS ionization efficiency is essential.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_HILIC_Method_Step_Procedure_for_Oligos_f3b9ea6b08.webp" alt="KNAUER: Table 1 - HILIC Method Step Procedure for Oligos." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_HILIC_Method_Step_Procedure_for_Oligos_f3b9ea6b08.webp 245w," sizes="100vw" width="945" height="357"><h4>Practical Tips for High-Performance HILIC</h4><h5>Maintain a Stable Water Layer</h5>A minimum of ~5% water is necessary at the start of the run. Too little water can eliminate retention entirely. Adjust water content carefully to fine-tune retention.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Water_Adjustment_Principle_for_HILIC_c0d8616f20.webp" alt="KNAUER: Figure 3 - Water Adjustment Principle for HILIC." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Water_Adjustment_Principle_for_HILIC_c0d8616f20.webp 245w," sizes="100vw" width="951" height="374"><h5>Use High Organic Content</h5>HILIC typically operates best with 70–95% acetonitrile. Polar analytes are retained more effectively under these conditions.<h5>Include a Volatile Buffer</h5>Use low concentrations (5–20 mM) of ammonium formate or acetate to:<ul><li>Stabilize pH</li><li>Reduce peak tailing</li><li>Maintain MS compatibility</li></ul><h5>Control Injection Solvent</h5>Avoid high-water sample solvents. They disrupt the partitioning mechanism and distort peaks.<h5>Allow Proper Column Equilibration</h5>HILIC columns require longer equilibration times than reversed-phase columns — typically 10–20 column volumes after major gradient or solvent changes.<h5>Consider Temperature</h5>Operating at 30–50 °C can:<ul><li>Improve peak shape</li><li>Reduce mobile phase viscosity</li><li>Stabilize retention behavior</li></ul><h4>From Alternative to Essential? Final Perspective</h4>HILIC-MS is steadily advancing as a strong, ion-pair-free option for oligonucleotide analysis. While IP-RP-LC still dominates in sensitivity for many applications, HILIC is closing the gap and offering valuable flexibility — particularly for highly polar or challenging sequences.Although HILIC follows different chromatographic principles than reversed-phase separations, once optimized, it can deliver robust and highly selective results.Rather than being a niche method, HILIC is becoming a strategic tool in modern oligonucleotide workflows — especially when MS compatibility and method versatility are priorities.If you are developing or refining an oligonucleotide LC-MS method, HILIC may be worth integrating into your analytical toolbox.<blockquote><ul><li>For additional information or discussion, feel free to contact: <a>sales@knauer.net<svg><use></use></svg></a></li><li>For direct scientific inquiries, reach out to the author at: <a>kindler@knauer.net<svg><use></use></svg></a></li></ul></blockquote>Stay tuned for more insights in the “Oligos Made Easy” series.

Oligos Made Easy - Part 4: HILIC

News from LabRulezLCMS Library - Week 09, 2026

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For more than 40 years, ALS has provided testing and technical solutions to clients all over the world in a wide range of industries. We have built our global reputation by embracing the latest technologies and innovative methodologies to deliver high quality testing services to clients. ALS is able to leverage a multifaceted international team while remaining agile enough to provide local expertise and personalized solutions to every one of our clients.

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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UV-Vis Thermal Melts can be a time consuming bottle-neck in the lab. We will discuss how melt measurements can instead be collected rapidly and accurately with the <a href="https://lcms.cz/products/628">Cary 3500 UV-VIS Spectrometer</a>.
<blockquote>
Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.
</blockquote>

Agilent: Save Time With Fast UV-Vis Thermal Melts

Save Time With Fast UV-Vis Thermal Melts

<h4>Overview</h4>
To remain competitive against other technologies, mass spectrometry (MS) needs to rise to the challenge of unprecedented throughput with very deep analyses of very complex samples. With analysis time reduced to several minutes by fast liquid separations, confident identification and quantitation over many orders of dynamic range still requires a generational leap in the sequencing performance of MS. The Thermo Scientific™ Astral™ analyzer is a novel class of high-resolution accurate-mass analyzer that compliments the Thermo Scientific™ Orbitrap™ analyzer to provide high speed and sensitivity measurements.
This webinar will explore the underlying technology of the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer and its’ application to biomedical research. Rethink what is possible with a mass spectrometer including the analysis of 300 proteomics samples per day, measuring 5,000 proteins from a true single-cell, and collecting fragmentation data on over 90% of compounds in metabolomics experiments.
Key Learning Objectives:
<ul>
<li>Learn about the novel technology of the Orbitrap Astral mass spectrometer </li>
<li>Understand the benefits of the Orbitrap Astral mass spectrometer </li>
<li>How to apply the Orbitrap Astral mass spectrometer to solve biological questions</li>
</ul>
Who Should Attend:
<ul>
<li>Academic Researchers </li>
<li>R&amp;D Scientists </li>
<li>Lab Technicians</li>
<li>Principal Investigators</li>
<li>Lab Managers</li>
</ul>
<blockquote>
Presenter: Dr. Alexander Makarov (Director, Global Research LSMS, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Dr. Hamish Stewart (Staff Scientist, Analytical Instruments Group, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Ann Thayer (Contributing Editor, C&amp;EN Media Group)
</blockquote>

C&EN: Pushing the Frontiers of Mass Spectrometry

Pushing the Frontiers of Mass Spectrometry

This webinar will explore the underlying technology of the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer and its’ application to biomedical research.

Join our webinar and learn more of how the Evosep technology, its workflows and applications in the Proteomics Core Facility.

Evosep Biosystems: Evosep One in the Proteomics Core Facility

Evosep One in the Proteomics Core Facility

Join our webinar and learn more of how the Evosep technology, its workflows and applications in the Proteomics Core Facility.

Ion chromatography (IC) has become the preferred method for ion analysis, replacing many time-consuming and laborious wet-chemical techniques such as gravimetry, nephelometry, turbidimetry, etc. IC is a multi-parameter method and can be used for the determination of a wide array of anions, cations, transition metals, amines, carbohydrates, and organic acids.
In fact, the United States Pharmacopeia has modernized a substantial number of monographs to stipulate IC methods for numerous pharmaceutical assays of APIs as well as analysis of impurities and excipients. In a joint webinar on March 6, Dr. Raman, Director, Analys Lab, India, and Niranjan Kothandaraman, Product Manager Ion Chromatography at Metrohm India, will introduce you to applied ion chromatography. They present a number of applications to determine typical anions and cations, among them nitrite and nitrate, the precursors of nitrosamines. 
After their presentation, they will address any questions you may have in a Q&amp;A session at the end of the webinar. Register for this event now and get to know the potential of IC for your daily routine analytics!
Key learnings of this webinar:
<ul>
<li>Learning what ion chromatography is and how it can be used for identification and quantification of assay, impurities, and excipients</li>
<li>Understanding validated IC methods for pharmaceutical applications</li>
<li>Getting to know an IC method to determine nitrite and nitrate, the precursors of nitrosamines</li>
</ul>
Who should attend?
R&amp;D managers, QC managers, lab technicians and chemists responsible for R&amp;D and QC in research, development, and manufacturing of pharmaceuticals.
<blockquote>
Presenter: Dr. Raman (Director Laboratory, Analys PLtd., India)
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<blockquote>
Dr. Raman is the Director of Analys laboratory private limited, Hyderabad. Dr. Raman has earned his doctorate in analytical chemistry from Acharya Nagarjuna University, Guntur, India. He has more than three decades of experience in analytical R&amp;D in pharmaceutical industry. Dr. Raman has been a member of the board of studies in educational institutions. He has more than 25 publications to his credit on the development and validation of analytical methods in HPLC, UPLC, IC and GC-MS techniques.
</blockquote>
<blockquote>
Presenter: N. Kothandaraman (PM Ion Chromatography, Metrohm India PLtd.)
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Mr. Niranjan Kothandaraman holds a M.Sc. degree in Applied Chemistry from Regional Engineering College, Trichy, India. Joined as an Application Chemist at Metrohm India and has good knowledge and working experience with all the analytical instruments from Metrohm AG. Having got an opportunity in marketing, currently he is working as a Product Manager at Metrohm India for Ion Chromatography, Voltammetry and Elga Water Purification Systems and is responsible for pan India business. He has been with Metrohm India since last 24 years.
</blockquote>

Metrohm: From APIs to Impurities and Excipients – Ion Chromatography for Pharmaceutical Analysis

From APIs to Impurities and Excipients – Ion Chromatography for Pharmaceutical Analysis

In a joint webinar we will introduce you to applied ion chromatography.

Ion Chromatography for Pharmaceutical Analysis

PhenoAcademy Webinars
 Liquid Chromatography Series
<h4>A Guide to Building Your LC Method Development Kit Plus Tips on Storage &amp; Care</h4>
<ul>
<li>Learn how to choose different column phases for developing a LC method</li>
<li>Understand what kind of LC columns can be a strong method development kit</li>
<li>Identify how to take care of your LC column to extend its lifetime</li>
</ul>
<blockquote>
Presenter: Kevin Zhao (Senior Technical Specialist)
</blockquote>
<blockquote>
Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.
</blockquote>
<blockquote>
Presenter: Koen Askamp (Technical Specialist)
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<blockquote>
Koen is from the Netherlands, studied Molecular Life Sciences at Wageningen University (Netherlands), majoring in immunology. Analytical Chemistry was one of my favorite courses – who doesn’t love solving puzzles?
</blockquote>
<blockquote>
I like drumming, scuba diving, motorcycle-riding, and this past winter I learned how to ski (a little)!
</blockquote>
<blockquote>
Presenter: Namrata Saxena (Technical Manager)
</blockquote>
<blockquote>
Namrata Saxena is a Technical Manager - Asia/Pacific” with Phenomenex and is based in Sydney, Australia. She has a post graduate degree with a specialization in Analytical Chemistry, with extensive experience in the Liquid chromatography domain, and her current focus is in Omics and Biologics field. 
</blockquote>
<blockquote>
Before joining Phenomenex she had worked as “Product Specialist” and spent several years in column chemistry, column manufacturing and method development in the application and manufacturing labs of Bischoff Chromatography, Germany. 
</blockquote>
<blockquote>
In her current role, she provides product selection, method development, training and troubleshooting support to customers as well as Phenomenex Sales Consultants globally. 
</blockquote>

Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

A Guide to Building Your LC Method Development Kit Plus Tips on Storage & Care

Learn how to choose different column phases for developing a LC method.

Building LC Method Development Plus Tips on Storage & Care

In today&#39;s fast-evolving laboratory landscape, lab safety is paramount. Insights from industry professionals emphasize the critical role of environmental, health, and safety (EHS) in shaping the priorities of today&#39;s labs. Labs today are facing challenges that were unimaginable a few years ago. To stay productive, they need every advantage they can get. That&#39;s why maintaining high EHS standards is a smart business decision. A Safe, Healthy Lab Is a Productive Lab.
In this webinar, we will delve into the essential elements of lab safety and explore the key priorities of today&#39;s labs. You will gain insights into the challenges labs encounter and discover proactive solutions to create the safest lab environment. Your lab&#39;s safety is our priority.
<h5>Webinar Focus Areas:</h5>
<ul>
<li>Importance of Safety</li>
<li>Chemical Safety</li>
<li>Ergonomics</li>
<li>Electrical Hazards</li>
<li>Environmental Stewardship</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Lab Managers</li>
<li>EHS Managers</li>
<li>Scientists</li>
<li>Anyone involved in lab safety decision-making</li>
</ul>
<blockquote>
Presenter: Mr. Sunil B Lakhmapure (Technical Support Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Sunil holds 19 year plus experience in the analytical field with major exposure to LC/GC with Single Quad, Triple Quad and high resolution (QToF) mass spectroscopy. Started career in Pharma R&amp;D as a scientist with hands on experience on high end instruments like LC/MS, XRD &amp; NMR. Developed &amp; published application notes using Chromatographic techniques on real world samples/issues at various levels.
</blockquote>

Agilent: Safe Lab: Lab Safety, Productivity, and Excellence at Your Fingertips

Safe Lab: Lab Safety, Productivity, and Excellence at Your Fingertips

In this webinar, we will delve into the essential elements of lab safety and explore the key priorities of today's labs.

Lab Safety, Productivity, and Excellence at Your Fingertips

<h4>Live Interactive Web Series</h4>
We’ll work through a topic of the week (roughly 60 minutes) and discuss any questions that come up along the way. During these sessions, we’ll talk about how to care for your SEC-MALS or FFF-MALS system, help you brush up on your ASTRA and DYNAMICS data processing, discuss measuring dn/dc values and why dn/dc is important, and see how stable RI baselines are achievable.
Working with polydisperse samples? We’ll see how to leverage ASTRA’s distribution analysis and results fitting features. Special topics on error analysis, batch MALS work, and more.
The live interactive web series are 100% live sessions, with anonymous Q&amp;A welcome during and after the discussion. Free for all to attend.
<ul>
<li>Part 1 of 6: Why we love Multi-Angle Light Scattering! How MALS works and what it measures. Modes of operation and when to use them*
<ul>
<li>MALS theory and operating principles</li>
<li>Batch MALS vs. flow mode MALS: when and why</li>
<li>What MALS can and cannot measure directly</li>
</ul>
</li>
<li>Part 2 of 6: Getting to know your MALS hardware, including ‘proper care &amp; feeding’ of the system, practical guides for optimizing data quality, and how to know when your DAWN hardware needs special attention*
<ul>
<li>Understanding the MALS hardware internal and external components</li>
<li>Diagnosing hardware issues</li>
<li>How to help your MALS operate at its best</li>
</ul>
</li>
<li>Part 3 of 6: Let it flow - Leveraging MALS with Size Exclusion Chromatography SEC / Gel Permeation Chromatography GPC. Considerations for successful SEC-MALS / GPC-MALS operation. How to process ASTRA data on and after day 1*
<ul>
<li>Practical considerations for MALS in SEC/GPC mode</li>
<li>Understanding system settings &amp; coefficients such as inter-detector band broadening and normalization</li>
<li>Squeezing the most information from your SEC-MALS data</li>
</ul>
</li>
<li>Part 4 of 6: Advanced topics in ASTRA data analysis - uncertainty calculations, MW averages, mass recovery, distribution analysis, results fitting, and data exporting*
<ul>
<li>Understanding the ways ASTRA tracks and calculates system errors</li>
<li>Leveraging peak integration to understand what’s in your sample</li>
<li>When and why to use the various MW averages: Mn, Mw, Mz, Mp, Mavg, Mv, etc.</li>
<li>Efficient and easy ways to export exactly the data and results you want, right out of ASTRA</li>
</ul>
</li>
<li>Part 5 of 6: All about dn/dc values and concentration detectors (UV and RI) for MALS*
<ul>
<li>Understanding dn/dc, what it is, and how it is used with MALS</li>
<li>Measuring / determining dn/dc for your samples</li>
<li>UV detectors and their role in MALS</li>
<li>Mass recovery in ASTRA</li>
</ul>
</li>
<li>Part 6 of 6: MALS flow cell care: diagnosing and cleaning your flow cell*
<ul>
<li>How to avoid the need to clean your MALS flow cell in the first place</li>
<li>How to diagnose a dirty MALS flow cell</li>
<li>How to take apart and clean your MALS flow cell</li>
</ul>
</li>
</ul>
*It is recommended but not required that you attend all the sessions in this series.
<blockquote>
Presenter: Dr. Andy Meyer
</blockquote>
<blockquote>
Dr. Andy Meyer joined Wyatt Technology in 2001, serving for several years as an Applications Scientist, then as Dean of Wyatt&#39;s &quot;Light Scattering University&quot; and as Director of Customer Service &amp; Support. After managing multiple regions in North America, Dr. Meyer is now a Senior Application Scientist for Wyatt. As a Wyatt customer, he completed his Ph.D. in Applied Science – Polymer Chemistry from the College of William and Mary in Virginia.
</blockquote>

Waters Corporation: Why we love Multi-Angle Light Scattering! How MALS works and what it measures. Modes of operation and when to use them

Why we love Multi-Angle Light Scattering! How MALS works and what it measures. Modes of operation and when to use them

Why we love Multi-Angle Light Scattering!

Meaningful biomarker studies that account for genetic, environmental and lifestyle variation, or comprise of complex signatures from multiple proteins, require large cohorts – too large for the current proteomics discovery platforms.
In this webinar, you’ll hear how Prof. Dr. Hanno Steen’s lab at Harvard Medical School has developed a novel high-throughput plasma proteomics pipeline designed for large-scale biomarker discovery studies.
Prof. Dr. Steen will highlight how the pipeline has been tested and validated on over 10,000 samples across diverse disease contexts, and outline how it could be used to better understand the impact of development, infection and vaccination on the immune system.
<h5>Attend this webinar to:</h5>
<ul>
<li>Discover how the novel pipeline uses chemical depletion to provide low-cost, high-throughput access to plasma proteomes in both model systems and humans</li>
<li>Hear how this method has identified unique immunophenotypes and prognostic biomarkers in diseases including COVID-19</li>
<li>Explore how the approach compares and contrasts with traditional antibody-based approaches to biomarker discovery</li>
</ul>
<blockquote>
Presenter: Prof. Dr. Hanno Steen (Associate Professor of Pathology Principal Investigator in the Department of Pathology and Director of Proteomics Harvard Medical School)
</blockquote>

Bruker: High-Throughput Plasma Profiling for Enhanced Biomarker Discovery

High-Throughput Plasma Profiling for Enhanced Biomarker Discovery

In this webinar, you’ll learn about novel high-throughput plasma proteomics pipeline designed for large-scale biomarker discovery studies.

High-Throughput Plasma Profiling for Biomarker Discovery

Design your new laboratory for success and progress your project quickly and efficiently. 
Join this webinar to learn how you can overcome common challenges when designing, developing and building a new laboratory for success in Biotechnology. 
You will hear real-world experiences and obtain key advice from people who have recently set up working laboratories for a range of innovative Biotechnology workflows. Additionally, Thermo Fisher Scientific will demonstrate the 3D Lab Design tool – a key asset to help you build your plan. 
<h5>Topics:</h5>
<ul>
<li>Round table discussion – key challenges when setting up a new lab</li>
<li>Optimising your lab around your specific needs – using the Thermo Scientific 3D Lab Design tool</li>
<li>Developing an effective laboratory and addressing unforeseen issues</li>
</ul>
<blockquote>
Presenter: Arfan Nazir (Lab Planner, Senior Account Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Fadi Adas (Business Development Manager, New Labs, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Stephan Jenn (President, NovAlix)
</blockquote>
<blockquote>
Presenter: Dr Kath Mackay (Director of Life Sciences, Bruntwood SciTech)
</blockquote>

ThermoFisher Scientific: Knowledge Culture Webinars: Setting up a new laboratory for Biotech success

Setting up a new laboratory for Biotech success

Join this webinar to learn how you can overcome common challenges when designing, developing and building a new laboratory for success in Biotechnology. 

Join us as we delve into the essential principles of Capillary Electrophoresis (CE) and explore its diverse applications across various scientific disciplines. Our industry expert Nandakumar Sundaramoorthy, Ph.D. will share valuable insights and practical knowledge to enhance your understanding of CE.
<h5>Discussion points:</h5>
<ul>
<li>CE and CE-MS: general theory, instrumentation and it&#39;s classification, applications and therapeutic areas</li>
<li>Significance of CE in the world of biotherapeutics and other bio-related products</li>
<li>Breakthrough of CE-based separation in novel biologics</li>
<li>Case study: CE-SDS and cIEF/CZE related problems, root cause and solutions</li>
<li>Quality control, regulatory expectations, and pharmacopeial evolution for CE-based methods</li>
</ul>
<blockquote>
Presenter: Dr. Nandakumar Sundaramoorthy
</blockquote>
<blockquote>
He is a scientific professional with nearly two decades of global experience, holding a Ph.D. in Applied Proteomics. He is currently the Director - Analytical Development and Characterization at Kemwell Biopharma Pvt Ltd. He has a proven track record of successfully leading end-to-end projects, specializing in protein characterization.
</blockquote>

SCIEX: Fundamentals of capillary electrophoresis (CE)

Fundamentals of capillary electrophoresis (CE)

Seznamte se s námi se základními principy kapilární elektroforézy (CE) a prozkoumejte její rozmanité aplikace v různých vědních oborech.