<div><p><strong>Protein purification is a critical step in biopharmaceutical research, proteomics, and industrial biotechnology. It involves isolating a specific protein from complex biological mixtures while preserving its native structure and activity. Traditional methods such as precipitation or dialysis lack the precision required for high-purity protein isolation, especially when dealing with therapeutic targets or complex protein assemblies.</strong></p><p>High-performance liquid chromatography (HPLC) protein purification has transformed this field by providing high resolution, reproducibility, and speed, making it indispensable for both analytical and preparative workflows.</p></div><div><div><div><div><h3><strong>What is HPLC Protein Purification?</strong></h3><p>HPLC purification is based on the principle of selective interactions between proteins and the stationary phase under high-pressure liquid flow. The separation relies on differences in size, charge, hydrophobicity, or affinity of proteins toward the stationary phase material.</p><p>Unlike conventional low-pressure methods such as gel filtration or affinity batch purification, HPLC purification operates under high pressure (up to 6000 psi), enabling faster separation with enhanced resolution and quantitative recovery. It also offers precise control over parameters like gradient composition, pH, and temperature, minimizing variability between runs.</p><p>In contrast to traditional column chromatography, HPLC uses smaller stationary phase particles (3–10 µm), resulting in increased surface area and sharper <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/how-to-reduce-peak-tailing-in-hplc"><strong>peak shapes</strong></a>, which are vital for protein purity assessment and scale-up purification.</p></div></div></div></div><div><div><div><div><h3><strong>Types of HPLC Used for Protein Purification</strong></h3></div></div></div></div><div><p>Different HPLC modes can be employed depending on the physicochemical properties of the target protein. Each method offers unique advantages in terms of selectivity and recovery.</p><h4><strong>Reversed-Phase HPLC (RP-HPLC)</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase"><strong>Reversed-Phase HPLC</strong></a> separates proteins based on hydrophobic interactions between the analyte and the stationary phase, typically C4, C8, or C18 silica-based columns. Proteins are eluted using gradients of organic solvents (usually acetonitrile or methanol) in the presence of volatile acids like formic or trifluoroacetic acid (TFA).</p><p>This method provides exceptional resolution and is commonly used for protein fragment purification, and analysis of recombinant proteins. For example, reversed-phase HPLC is routinely employed in peptide therapeutic workflows, such as the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an59280424_w_fc6c3ac6f0.pdf"><strong> Tirzepatide Preparation and Purification </strong></a>process, where purity and recovery are critical to product efficacy. However, due to exposure to organic solvents, RP-HPLC may cause partial denaturation, limiting its use for native proteins.</p><h4><strong>Ion Exchange HPLC (IEX-HPLC)</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec"><strong>IEX-HPLC</strong></a> separates proteins based on net surface charge. Cation exchange (with negatively charged stationary phases) binds positively charged proteins, while anion exchange does the reverse.</p><p>By gradually increasing salt concentration or changing the mobile phase pH, proteins are eluted according to their isoelectric points (pI).By gradually increasing salt concentration or changing the mobile phase pH, proteins are eluted according to their isoelectric points (pI).</p><p>This approach is highly suitable for native purification, protein isoform analysis, and charge variant characterization essential steps in therapeutic protein production.</p><h4><strong>Size Exclusion Chromatography (SEC-HPLC)</strong></h4><p>SEC separates molecules based on their hydrodynamic radius. Larger proteins elute first, as they are excluded from the pores of the stationary phase, while smaller molecules penetrate and elute later.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec"><strong>SEC-HPLC</strong></a> is non-denaturing and ideal for determining protein molecular weight, oligomeric state, and aggregate content. It is frequently used as a final polishing step following ion exchange or affinity chromatography.</p><h4><strong>Affinity HPLC</strong></h4><p>Affinity-based HPLC leverages specific biological interactions between the target protein and a ligand immobilized on the stationary phase (e.g., antibodies, metal ions, or tags such as His-tag or GST).</p><p>When integrated into an HPLC system, affinity chromatography provides high selectivity and purity (&gt;95%) in a single step, often used in recombinant protein purification and biomarker capture applications.</p></div><div><div><div><div><h3><strong>Benefits of Using HPLC for Protein Purification</strong></h3><p>HPLC-based protein purification combines the analytical precision of chromatography with the preparative capability needed for research and biomanufacturing. The main advantages include:</p><h5><strong>Benefit &nbsp;/ &nbsp;Description</strong></h5><p><strong>High Resolution and Selectivity:</strong></p><ul><li>Smaller stationary phase particles and optimized flow dynamics allow HPLC to resolve proteins differing by minimal physicochemical properties. This precision is vital for distinguishing isoforms, mutants, and post-translationally modified variants.</li></ul><p><strong>Accuracy and Reproducibility</strong></p><ul><li>Automated systems maintain stable flow rates, gradient control, and detection sensitivity, ensuring reproducible results essential for regulated biopharmaceutical workflows.</li></ul><p><strong>Speed and Efficiency</strong></p><ul><li>Modern UHPLC systems equipped with fully porous or core–shell particles achieve faster separations without sacrificing resolution, reducing run times by up to 50% compared to classical methods.</li></ul><p><strong>Compatibility and Scalability</strong></p><ul><li>HPLC systems are highly adaptable, supporting both analytical-scale characterization and preparative-scale purification. Methods developed at microgram scale can be seamlessly transferred to milligram or gram scales used in process development.</li></ul><div><div><div><div><h3><strong>Factors Affecting the Efficacy of Protein Purification by HPLC</strong></h3></div></div></div></div><div><p>Optimizing HPLC for protein purification requires balancing purity, recovery, and throughput. The following parameters significantly influence results:</p><ul><li><strong>Purification goal:</strong> Define whether the priority is yield, purity, or throughput. Analytical separations prioritize resolution, while preparative runs emphasize recovery and process speed.</li><li><strong>Protein solubility and stability:</strong> Unstable or aggregation-prone proteins may require low temperatures or additives (e.g., glycerol, mild detergents).</li><li><strong>Column chemistry and particle size:</strong> Select appropriate stationary phase chemistry (silica, polymeric, or mixed-mode) and particle size to suit molecular weight and hydrophobicity.</li><li><strong>Mobile phase pH and ionic strength:</strong> pH should be optimized near but not at the protein’s pI; salt gradients influence both resolution and elution strength.</li><li><strong>Flow rate and gradient profile:</strong> Steeper gradients shorten run time but may compromise separation quality.</li><li><strong>Purified protein final form:</strong> Consider buffer composition and concentration for storage or downstream assays to maintain bioactivity.</li></ul></div><div><div><div><div><h3><strong>Best Practices for Protein Purification by HPLC</strong></h3></div></div></div></div><div><p>Following good chromatographic practices ensures consistent, high-quality results:</p><ul><li><strong>Column conditioning and storage:</strong> Always equilibrate new <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=48&amp;manufacturerItems=50"><strong>HPLC columns</strong></a> with 10 column volumes of buffer; store in appropriate solvent containing preservatives.</li><li><strong>Avoid protein precipitation:</strong> Maintain appropriate ionic strength and, when native state is desired, avoid exposure to denaturing solvents or high organic content.</li><li><strong>Prevent sample loss:</strong> Use low-protein-binding tubing and vials; minimize dead volume in the system.</li><li><strong>Troubleshooting:</strong><ul><li>Peak fronting - may indicate column overloading.</li><li>Peak broadening – may indicate column channeling.</li><li>Carryover – reduce by extending wash cycles or using stronger regeneration solvents.</li><li>Clogging – prefilter samples (0.22 µm) and periodically flush the system.</li></ul></li></ul></div></div></div></div></div>

Protein Purification by HPLC: Methods, Benefits, and Best Practices

<p><strong>Scientists from CEITEC Brno University of Technology&nbsp;have succeeded in a highly competitive international selection process and secured a prestigious Human Frontier Science Program (HFSP) grant, which supports groundbreaking fundamental research. The Brno-based team led by Eric Daniel Glowacki will contribute a unique technology to an international project that enables precise control of oxygen levels in tissues. Changes in oxygen availability may play a crucial role in determining how cells specialize during early embryonic development.</strong></p><p>The project, entitled “Beyond Theory: Regulation of Mammalian Embryo Patterning by Electrochemical Gradients,” is being carried out by CEITEC BUT in collaboration with biologists from the <strong>Max Planck Institute of Molecular Cell Biology and Genetics</strong> and <strong>Yale University</strong>. The Brno team’s role is central to the project: providing technology that enables the deliberate creation of regions with different oxygen concentrations in tissues and the observation of how cells respond to these changes.</p><p><i>“Our research group specializes in electrochemistry, particularly in studying how electric current affects tissue oxygenation and the resulting chemical changes. To perform certain types of experiments, we developed our own integrated system of needle probes, electrodes, and software, which allows us to influence and control the amount of oxygen present in tissues through which current is passed,” </i>explains team leader <strong>Eric Daniel Glowacki</strong>. Using these tools, researchers can create regions within samples where oxygen is scarce or even completely absent. Changes in oxygen levels are fundamental to biology because they affect cellular metabolism and, consequently, cell behaviour. In some types of research, such precise environmental control is essential.</p><p><i>“Our system is not universal; we tailor it to each application. In the future, however, we aim to expand its variants so that it can be used across the widest possible range of research fields,”</i> adds Eric.</p><p>Scientists at the Max Planck Institute have already used CEITEC’s technology in previous studies of early embryonic development. They investigated how the metabolic activity of stem cells – undifferentiated cells capable of self-renewal and transformation into specialized cells such as muscle, nerve, or blood cells – affects both their own development and that of neighbouring cells.</p><p>The newly funded HFSP project builds on this work. Its goal is to revisit the nearly century-old theory&nbsp;proposed by the American zoologist Charles Manning Child, which explains how stem cells in multicellular organisms gradually develop into mature cells with specific functions and structures. The three-year project, supported by a grant of<strong> CZK 7.5 million </strong>for each partner, will test a new hypothesis in mouse embryos directly in the uterus: that the specific transformation of cells depends not only on oxygen depletion itself but also on the cell’s location within the tissue and the timing of the oxygen decrease.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Technologie_vedcu_z_CEITEC_pomuze_odhalit_jak_kyslik_ridi_vyvoj_embryi_2_1ea86be296.jpeg" alt="CEITEC/ Lukáš Havlák: CEITEC Technology Will Help Reveal How Oxygen Controls Embryo Development: HFSP technological setup at CEITEC BUT." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Technologie_vedcu_z_CEITEC_pomuze_odhalit_jak_kyslik_ridi_vyvoj_embryi_2_1ea86be296.jpeg 235w," sizes="100vw" width="1600" height="1067"></p><p>A better understanding of the mechanisms that govern early embryonic development could, in the future, contribute to advances in regenerative medicine, research into developmental disorders, and the more effective use of stem cells in biomedicine.</p><p><i>“The success of our project is both a great honour and a significant responsibility. HFSP is a unique programme dedicated to fundamental research, where investigators seek answers to the most basic and still unresolved questions in biology,”</i> says Eric. The programme is extremely competitive, with only two to three percent of the thousands of submitted projects receiving funding. Requirements include international collaboration across continents and a genuinely innovative approach.</p><p><i>“Through this, we have become part of a community of world-class scientists from around the globe. Although the results of this kind of research are not immediately applicable, their long-term benefit to society is immense,” </i>Eric concludes.</p><blockquote><h4><strong>About HFSP</strong></h4><p>The Human Frontier Science Program Organization (HFSPO) was established in France in 1989, although the idea of creating such an international community dedicated to fundamental research originated several years earlier in Japan. The programme funds cutting-edge research in life sciences, particularly in biology, physics, chemistry, mathematics, and computer science, and supports innovative interdisciplinary projects that would otherwise be difficult to finance.</p><p>HFSPO receives funding from governments and institutions, including those of France, Japan, Switzerland, South Korea, the United States, the European Union, Singapore, New Zealand, and the United Kingdom. Since the programme’s launch in 1989, 31 HFSP-supported scientists have gone on to receive Nobel Prizes. The most recent awardees, in 2024, were David Baker, who received the Nobel Prize in Chemistry for computational protein design, and Gary Ruvkun, who received the Nobel Prize in Physiology or Medicine for the discovery of microRNA.</p></blockquote>

CEITEC Technology Will Help Reveal How Oxygen Controls Embryo Development

<p><strong>🎤Rajas Poorna and Charles Anderson</strong></p><h4><strong>What if you could dry biofluid samples in the field—without a vacuum system, freeze dryer, or full lab setup?</strong></h4><p>In this episode of Concentrating on Chromatography, I sit down with Rajas Poorna and Charles Anderson to explore their work on a frugal, field-deployable alternative to traditional lyophilization through the Evapinator project.</p><p>Originally inspired by the principles of frugal science, their approach rethinks how we stabilize and prepare biofluid samples like blood and plasma—especially in environments where traditional lab infrastructure simply isn’t available.</p><h4><strong>What This Episode Covers:</strong></h4><h5><strong>A Frugal Take on Lyophilization</strong></h5><p>Can you replicate the outcome of a lyophilizer without the complexity?<br>Why traditional freeze drying is often too expensive and impractical for field use<br>How simplifying evaporation could unlock entirely new workflows</p><h5><strong>Drying Biofluids in the Field</strong></h5><p>The real-world challenge of handling blood and other biofluids outside the lab<br>Why sample stability during transport is such a critical bottleneck<br>How a portable system could enable on-site sample prep in remote or resource-limited settings</p><h5><strong>Rethinking Evaporation (No Vacuum Required)</strong></h5><p>The core idea behind the Evapinator—and how it challenges conventional assumptions<br>The role of airflow, heat, and design in drying sensitive samples<br>Where this approach fits relative to traditional evaporation and lyophilization techniques</p><h5><strong>Early Results &amp; Practical Insights</strong></h5><p>What’s working so far—and where limitations still exist<br>The types of applications where this approach shows the most promise<br>What still needs to be validated in upcoming peer-reviewed work</p><h4><strong>Why This Matters</strong></h4><p>From clinical research in remote regions to decentralized diagnostics, the ability to dry and preserve biofluid samples in the field could fundamentally change how and where analysis happens.</p><p>This conversation challenges a core assumption in analytical science:</p><p>Do you really need a full lyophilizer—or even a vacuum—to stabilize biofluid samples?</p><h3><strong>Video Transcription</strong></h3><p>The interview opens with a welcome to an episode of <i>Concentrating on Chromatography</i>. The host introduces guests <strong>Rajas Poorna</strong> and <strong>Charles Anderson</strong> and asks about their scientific backgrounds and how they became involved in solving evaporation challenges.</p><h4><strong>Scientific background and project origin</strong></h4><p>Rajas explains that he is trained as a physicist and is now pursuing a PhD in bioengineering. His lab focuses on building low-cost scientific equipment to make science more accessible, especially for schools and low-resource settings. This work is part of a broader “frugal science” approach, where students and researchers develop affordable versions of instruments that are normally expensive.</p><p>The original challenge came from synthetic biology education. High school teams working on synthetic biology projects needed access to equipment such as lyophilizers, but conventional systems can cost tens of thousands of dollars. The team therefore began exploring whether a more affordable lyophilizer-like system could be built for education and field use.</p><p>Charles describes joining the project early in his undergraduate studies. He was drawn to it because it had a clear practical purpose and seemed like something that could become useful outside the laboratory rather than remaining only a research prototype.</p><h4><strong>Why evaporation became the key challenge</strong></h4><p>The discussion turns to the practical limitations of traditional evaporation and drying systems. Rajas explains that during a collaboration in Thailand, researchers working on Raman spectroscopy for tuberculosis diagnostics needed to dry blood-derived samples before transporting them to a central laboratory. They used a centrifugal vacuum evaporator, but the system was slow, expensive, and difficult to service when it broke down.</p><p>This experience led to a central question: could sample drying be achieved without a vacuum? Rajas notes that everyday drying processes, such as drying clothes, do not require vacuum. This insight opened the path toward a simpler, smaller, lower-cost and potentially battery-powered drying system.</p><h4><strong>How Rajas and Charles began collaborating</strong></h4><p>Charles explains that he met the lab’s principal investigator while discussing a different project. He was then invited to work on what would later become the Evaponator. At the beginning, the project was still small and exploratory.</p><p>Rajas adds that Charles joined at a stage when the team was trying to understand the physics and engineering behind vacuum-free drying. Within a few months, they developed a prototype costing under 100 USD that could dry samples much faster than the conventional vacuum evaporator used by collaborators.</p><h4><strong>Main limitations of conventional drying systems</strong></h4><p>The interview identifies several major limitations of existing evaporation technologies:</p><ul><li>High cost</li><li>Limited reliability in remote or low-resource settings</li><li>Long drying times</li><li>Poor field portability</li><li>Challenges with sterility and cross-contamination</li></ul><p>Charles emphasizes that freeze drying can take 12–24 hours, which is impractical for field use. Rajas adds that centrifugal vacuum evaporators are not necessarily sterile and may pose a risk of cross-sample contamination. Together, these challenges shaped the requirements for the Evaponator.</p><h4><strong>What the Evaponator is</strong></h4><p>Charles introduces the Evaponator as a low-cost, portable drying system designed to dry small numbers of samples quickly without a vacuum chamber. The name was inspired by an episode of <i>Phineas and Ferb</i>.</p><p>The system is based on the same principles as drying clothes: low humidity, gentle heating and airflow. Instead of using vacuum, the Evaponator generates dry air, directs it over the sample, removes moisture through a cold trap and repeats the process in a closed or semi-closed loop.</p><p>The current design is aimed at drying approximately four 1 ml samples, which fits the intended use case of field diagnostics or low-throughput sample collection in remote settings.</p><h4><strong>How the system works</strong></h4><p>Rajas explains the operating principle in more detail. Moist air is passed through a cooled metal block using a thermoelectric cooler. As the air cools, moisture condenses inside the cold trap, producing dry air. This dry air is then directed over warmed samples, helping water evaporate while keeping the sample temperature moderate.</p><p>The system can operate in a loop to improve efficiency. HEPA filtration and aerosol filtration help prevent contamination. The team also developed an ozone-based sterilization approach, allowing the device to sterilize itself using ozone generated from air rather than relying on consumables such as ethanol or peroxide.</p><p>To prevent sample-to-sample contamination, the design uses separate airflow paths for individual samples, which merge only later in the system.</p><h4><strong>Design assumptions and engineering constraints</strong></h4><p>A key design assumption is that not every drying process needs sublimation or deep vacuum. For the biofluid applications targeted by the Evaponator, the team works around 40 °C, a temperature chosen because it is close to fever temperature and should be mild enough for many biological samples.</p><p>The engineering constraints included:</p><ul><li>Use of widely available components</li><li>No specialized machining</li><li>Bill of materials under 100 USD</li><li>Low power consumption</li><li>Portability</li><li>Simple assembly</li><li>Robust airflow without splashing or sample loss</li></ul><p>Charles notes that the team intentionally selected accessible parts, including components similar to those used in computer cooling systems, vacuum-cleaner-style HEPA filters and disposable pipette tips for directing airflow.</p><h4><strong>Sample types and solvents</strong></h4><p>The prototype is currently focused mainly on aqueous biological samples. Rajas explains that using organic solvents such as ethanol would introduce ignition risks with the current architecture, so the early work is focused on water-based samples and biofluids.</p><p>Charles adds that the system is intended primarily for blood-derived fluids, urine, saliva and similar samples where the target analytes can remain stable after drying and rehydration.</p><h4><strong>Potential analytical applications</strong></h4><p>The Evaponator is being developed for field sample preparation rather than as a replacement for every laboratory evaporator. The main idea is to dry samples near the point of collection, transport them more easily and then rehydrate them before analysis.</p><p>Potential use cases include:</p><ul><li>Field diagnostics</li><li>Biofluid sample stabilization</li><li>Remote sample collection</li><li>Clinical or public-health testing in low-resource settings</li><li>Applications where dried plasma, serum, urine or saliva can later be analyzed</li><li>Situations where conventional dried blood spot methods lack enough sample volume</li></ul><p>Rajas notes that red blood cells may not be suitable for drying directly because they can rupture, so serum or plasma is expected to be more appropriate. He also points out that oxygen exposure during forced-air drying still needs further testing for oxygen-sensitive analytes.</p><h4><strong>Current development status</strong></h4><p>Charles explains that the project is approaching important validation milestones, especially around batch-to-batch and sample-to-sample sterility. The ozone cleaning system appears promising because it may reduce the need for cleaning consumables, aside from inexpensive items such as pipette tips and occasional filter replacement.</p><p>He also mentions tests with trehalose-based solutions and collaboration with a Georgia Tech startup working on room-temperature-stable mRNA vaccines. In that context, the Evaponator was able to dry samples in about 30 minutes, compared with 12–24 hours using previous drying approaches.</p><h4><strong>Commercialization and future direction</strong></h4><p>Rajas explains that the team is exploring commercialization and has participated in a startup accelerator. They are investigating where the technology could have the strongest product-market fit, with particular interest from cost-sensitive and geographically dispersed healthcare settings, including India.</p><p>The goal is to move beyond a lab prototype toward a manufacturable, user-friendly device with a straightforward interface and reliable sealing, sterilization and operation.</p><h4><strong>Closing remarks</strong></h4><p>The interview concludes with the host thanking Rajas Poorna and Charles Anderson for sharing the technical and practical challenges behind the Evaponator. Both guests emphasize that the conversation was a valuable opportunity to explain the project in depth and reflect on its progress.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Frugal Science Meets Sample Prep: A Field-Ready Lyophilizer for Biofluids

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Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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<p><strong>PhenoAcademy Webinars</strong></p>
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<h4><strong>HPLC Troubleshooting</strong></h4>
<ul>
<li>Learn how to prevent and minimize your LC problems before they occur</li>
<li>Understand how to approach problem solving and troubleshooting in a logical way, including: diagnose cause and possible solutions</li>
<li>Review peak shape, and column performance problems</li>
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<blockquote>
<p><strong>Presenter</strong>: Kevin Zhao (Senior Technical Specialist)</p>
</blockquote>
<blockquote>
<p>Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.</p>
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<p><strong>Presenter</strong>: Koen Askamp (Technical Specialist)</p>
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<p>Koen is from the Netherlands, studied Molecular Life Sciences at Wageningen University (Netherlands), majoring in immunology. Analytical Chemistry was one of my favorite courses – who doesn’t love solving puzzles?</p>
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<p>I like drumming, scuba diving, motorcycle-riding, and this past winter I learned how to ski (a little)!</p>
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<p><strong>Presenter</strong>: Namrata Saxena (Technical Manager)</p>
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<p>Namrata Saxena is a Technical Manager - Asia/Pacific” with Phenomenex and is based in Sydney, Australia. She has a post graduate degree with a specialization in Analytical Chemistry, with extensive experience in the Liquid chromatography domain, and her current focus is in Omics and Biologics field. </p>
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<p>Before joining Phenomenex she had worked as “Product Specialist” and spent several years in column chemistry, column manufacturing and method development in the application and manufacturing labs of Bischoff Chromatography, Germany. </p>
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<p>In her current role, she provides product selection, method development, training and troubleshooting support to customers as well as Phenomenex Sales Consultants globally.  </p>
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Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

HPLC Troubleshooting

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<h4><strong>EU Live Interactive Web Series</strong></h4>
<p><strong>Gather on Thursday or Friday at 11 AM or 12 PM CET for our live interactive web series.</strong></p>
<p>Join us for an engaging journey into the world of advanced molecular analysis with our EU Live Interactive Web Series, hosted by Waters | Wyatt Technology! Dive deep into the intricacies of SEC-MALS and FFF-MALS systems, enhance your skills in ASTRA and DYNAMICS data processing, and master the measurement of dn/dc values—crucial for your research.</p>
<p>We dedicate 60 minutes to explore a specific topic, accompanied by a live discussion where all your questions are welcome. Whether you&#39;re dealing with polydisperse samples, eager to optimize your error analysis techniques, or curious about batch MALS work, our sessions are designed to elevate your expertise.</p>
<p>What&#39;s more, you’ll learn how to achieve stable RI baselines and utilize ASTRA&#39;s robust distribution analysis and results fitting features. Our web series is an invaluable, free resource exclusively for Wyatt customers, promising a 100% live, interactive learning experience.</p>
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<p>The live interactive web series are 100% live sessions, with anonymous Q&amp;A welcome during and after the discussion. Free for all Wyatt customers.</p>
<p>The EU live interactive web series are held on select Thursday or Friday afternoons at 11 AM or 12 PM CET.</p>
<p>May 3rd at 11:00 AM : MALS</p>
<ul>
<li>How MALS can characterize molar mass, radius and A2?</li>
<li>How does a DAWN detector work?</li>
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<p>May 17th at 11:00 AM : SEC-MALS</p>
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<li>Applications and data interpretation</li>
<li>Data analysis in ASTRA</li>
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<p><strong>June 7th at 11:00 AM : RI &amp; dn/dc</strong></p>
<ul>
<li><strong>A perfect match: MALS and RI</strong></li>
<li><strong>The importance of dn/dc and how to determine it</strong></li>
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<p>June 21st at 11:00 AM : DLS </p>
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<li>Benefits of measuring the Hydrodynamic Radius (Rh)</li>
<li>Dynamic Light Scattering in cuvette, high throughput and online</li>
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<p>July 5th at 11:00 AM : Dynamics data processing tips &amp; tricks</p>
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<li>Data interpretation and data filter</li>
<li>Use your Dyna Pro instrument to get: colloidal stability (kD/A2), thermal stability, viscosity or particle concentration</li>
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<p><strong>Presenter:</strong> Dr. Christian Ackerschott</p>
</blockquote>
<blockquote>
<p>Dr. Christian Ackerschott studied Chemistry at the University of Cologne and finished with his diploma thesis in Biochemistry. He completed his studies with the PhD thesis “Characterization of Recombinant Flagelliform Spider Silk Proteins” at the Technische Universität München, where AF4-MALS was used as an essential characterization method.</p>
</blockquote>
<blockquote>
<p>Since 2007, Christian works at Wyatt Technology as Application Scientist and is now Head of Customer Service. His tasks also include customer trainings in Dernbach and onsite as well as education and training of the local Field Application Scientists.</p>
</blockquote>


Waters Corporation: EU Live Interactive Web Series

RI & dn/dc

A perfect match: MALS and RI. The importance of dn/dc and how to determine it.

<p><strong>AAVs are attractive vehicles for targeted drug delivery. There are numerous Critical Quality Attributes (CQAs) evaluated to ensure safety and efficacy of AAVs. Aggregation is a CQA and should be controlled as AAVs are prone to aggregation from freeze thaw cycles during storage.</strong></p>
<ul>
<li>In this webinar, we will explore CQAs using a novel size exclusion column optimal for AAV analysis, which enables the separation of AAV monomer from aggregate species with excellent peak shape, resolution and peak profile to provide better data quality. Due to both the inert surface chemistry and flow-path in the BiozenTM dSEC-7 column, the analysis conditions were independent of the serotype showcasing the utilization of platform methodology.</li>
<li>In addition to calculating the aggregation level, aggregate molar mass, molar masses of protein and nucleic acid, and extinction coefficients of capsid and DNA were also determined. The novel particle chemistry of Biozen dSEC-7 provides instant stabilization of sensitive detectors like MALS to provide quality data.</li>
<li>Additionally, Full-to-Empty capsid ratio was determined to accurately predict capsid load demonstrating Biozen dSEC-7’s utility as a holistic solution for the determination of CQAs of AAVs.</li>
</ul>
<p><strong>Key Learning Points:</strong></p>
<ul>
<li>Learn how to optimize AAV aggregate analysis.</li>
<li>Understand how size exclusion can be effectively utilized for capsid load quantification.</li>
<li>Find out how optimized column specifications can improve data quality to determine critical quality attributes</li>
</ul>
<p><strong>Who should attend?</strong></p>
<p>Any scientist, associate or manager that optimizes AAV aggregate analysis methods.</p>
<blockquote>
<p><strong>Presenter:</strong> Claire Butré, PhD, (R&amp;D Technical Leader, Quality Assistance &amp; Ramkumar Dhandapani, PhD, Senior Product Management, Phenomenex)</p>
</blockquote>


Phenomenex: Evaluation of AAV Aggregation & Capsid Load (Empty / Full) CQAs

Evaluation of AAV Aggregation & Capsid Load (Empty / Full) CQAs

Explore CQAs using a novel SEC column for AAV analysis, which enables the separation of AAV monomer from aggregate species.

Evaluation of AAV Aggregation & Capsid Load CQAs

<p><strong>Limited understanding due to its complex pathophysiology and lack of definitive biomarkers currently constrains the diagnosis and treatment of Alzheimer’s disease (AD). But new research is uncovering dynamic brain changes during Alzheimer’s progression, offering potential therapeutic targets. This webinar explores how proteomics and systems biology can be integrated to elucidate AD pathology.</strong></p>
<p>You’ll learn how mass spectrometry-based proteomics provides insights into brain changes and assists biomarker discovery in cerebrospinal fluid and plasma, and discover the potential of affinity-based proteomics for reliable biomarker identification. Our expert speaker will delve into intricate networks like proteostasis and the matrisome, highlighting their implications for therapeutic development.</p>
<p><strong>Attend this webinar to:</strong></p>
<ul>
<li>Gain insights into the landscape of AD brain changes using unbiased mass spectrometry-based proteomics</li>
<li>Learn about biomarker discovery in AD cerebrospinal fluid and plasma through multi-platform proteomic analysis</li>
<li>Understand the potential of affinity-based proteomics, including modified aptamer technology, in identifying reliable biomarkers for AD</li>
<li>Explore the intricate networks associated with AD pathology and their implications for therapeutic development</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Erik Johnson (Assistant Professor, Department of Neurology, Emory University)</p>
</blockquote>


Technology Networks: Using Proteomics To Advance Understanding of Alzheimer’s Disease

Using Proteomics To Advance Understanding of Alzheimer’s Disease

You’ll learn how mass spectrometry-based proteomics provides insights into brain changes and assists biomarker discovery in cerebrospinal fluid and plasma.

Using Proteomics To Understand Alzheimer’s Disease

<p><strong>Next-generation sequencing (NGS) technologies enable deeper insights into different areas of research. In this new webinar series, researchers from Psomagen, Decode Health, and Agilent Technologies discuss how quality control steps in PacBio Hi Fi long-read sequencing and RNA sequencing support the discovery of new biomarker candidates for diagnostic and therapeutic applications.</strong></p>
<p>Assessing the quality of genomic DNA (gDNA) is of utmost importance in next-generation sequencing (NGS) workflows. Recently, there has been a growing interest in long-read sequencing, as it offers researchers the capability to analyze genomes more comprehensively and accurately. Traditionally, agarose gel electrophoresis/pulsed field gel electrophoresis (PFGE) has been the preferred quality assessment method for long-read sequencing. However, these techniques have limitations regarding sample usage and longer run times. Conversely, recent technological advancements have introduced instruments like the Agilent Femto Pulse system. These instruments can accurately characterize the size distribution of high molecular weight (HMW) DNA and SMRTbell libraries with faster run times and minimal DNA requirements, typically only picogram amounts. This webinar aims to provide an overview of the applications and key benefits of the Femto Pulse system, particularly its use in PacBio long-read sequencing workflows.</p>
<p><strong>Key learning objectives</strong></p>
<ul>
<li>Explore how to effectively assess the quality of genomic DNA (gDNA) in NGS workflows</li>
<li>Learn how the Agilent Femto Pulse system is used in PacBio long-read sequencing workflows</li>
<li>Find out how to determine the number of moles of a sample to better predict long-read sequencing read length</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Abhilasha Cheruku, MS (Product Manager-Genomic Services, Psomagen, Inc., United States)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Leslie Friedmann (Product Manager for Parallel CE instruments, Agilent Technologies, Germany)</p>
</blockquote>


Agilent: Analyzing High Molecular Weight (HMW) DNA Size Distribution for PacBio HiFi Long Read Sequencing Services using Agilent Femto Pulse System

Analyzing High Molecular Weight (HMW) DNA Size Distribution for PacBio HiFi Long Read Sequencing Services using Agilent Femto Pulse System

Applications and key benefits of the Femto Pulse system, particularly its use in PacBio long-read sequencing workflows.

Analyzing High Molecular Weight (HMW) DNA Size Distribution

<p><strong>MALDI Imaging allows for interrogation of the spatial distribution of metabolites and lipids in tissue, as well as expanding targeted analysis to small molecules, glycans, and targeted proteins. Recent technical developments enable imaging for maximum biological information at robust (sub)cellular resolution. However, imaging entire tissue sections with high spatial resolution entails large number of pixels and requires extensive acquisition times. Thus, researchers are seeking for alternative high-speed chemical imaging techniques, which are conducted prior to MALDI Imaging to narrow down the regions of interest (ROIs) on large sample areas. Infrared Laser Imaging (ILIM) allows non-destructive, label free biochemical imaging of complete tissue sections within minutes – thus represents the ideal complementary analytical tool to make MALDI Imaging faster and smarter.</strong></p>
<p><strong>Webinar Overview</strong></p>
<p>This webinar will introduce how breakthrough IR Laser Imaging performance is combined with best-in-class MALDI Imaging. Bruker’s new end-to-end workflow for IR Guided MALDI Imaging enhances sample throughput and provides increased analytical depth by offering maximized spatial resolution and collision cross section (CCS)-enabled label analysis for large-scale MALDI Imaging studies in biological and pharmaceutical research.</p>
<p><strong>What to Expect:</strong></p>
<p>Live presentation from Bruker’s experts to see the how tissue imaging has become faster than ever, the seamless integration of Infrared Laser Imaging (ILIM) into existing MALDI Imaging workflows and highlighting user stories, how IR Guided MALDI Imaging paves the way for deeper spatial multiomics of complex biological systems.</p>
<p><strong>Who Should Attend:</strong></p>
<ul>
<li>MALDI Imaging users wanting to expand their workflows for increased sample throughput and analytical depth.</li>
<li>Scientists &amp; researchers interested in conducting multimodal imaging approaches for increased biological insight.</li>
<li>Anyone interested in learning more about Infrared Laser Imaging and MALDI Imaging capabilities.</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Wojciech Kazmierski ( Country Manager, Bruker Optics, Poznan, Poland)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Arne Behrens, Ph.D. (Application Scientist Imaging, Bruker Daltonics, Bremen, Germany)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Domenic Dreisbach (Business Development Multimodal Imaging, Bruker Optics, Ettlingen, Germany)</p>
</blockquote>


Bruker: Make MALDI Imaging faster and smarter with Infrared Laser Imaging

Make MALDI Imaging faster and smarter with Infrared Laser Imaging

IR Laser Imaging combined with best-in-class MALDI Imaging.

MALDI Imaging with Infrared Laser Imaging

<p><strong>This webinar unveils the development of a high-throughput multidimensional liquid chromatography approach in combination with charged aerosol detection (CAD), leveraging a combination of ion exchange and reversed-phase columns, ensuring accurate and precise analysis within minutes.</strong></p>
<p>Discover how this method advances formulation development, stability testing, and batch release, providing researchers with valuable insights into surfactant degradation dynamics. </p>
<p>Learning Points: </p>
<ul>
<li><strong>Discover cutting-edge techniques for rapid analysis of surfactants in antibody formulations</strong></li>
<li><strong>Unveil high-throughput liquid chromatography with charged aerosol detection</strong></li>
<li><strong>Gain valuable insights for optimizing formulation development and stability testing</strong></li>
</ul>
<p>Who should attend?</p>
<ul>
<li><strong>Biopharmaceutical scientists and researchers specializing in monoclonal antibody development</strong></li>
<li><strong>Analytical chemists and technicians involved in pharmaceutical formulation analysis</strong></li>
<li><strong>Quality control and regulatory affairs professionals in the pharmaceutical industry</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Maksymilian Zegota, PhD (Senior Scientist, Coriolis Pharma)</p>
</blockquote>
<blockquote>
<p>Dr. Zegota earned his PH.D. in chemistry from the Max Planck Institute for polymer research in Mainz, Germany, concentrating on site-selective protein modification.</p>
</blockquote>
<blockquote>
<p>After completing his doctorate, he joined Coriolis Pharma in Martinsried, Germany.</p>
</blockquote>
<blockquote>
<p>Currently, Dr. Zegota focuses on developing Novel methods for surfactant analysis in biologics to assist in the root-cause analysis of polysorbate and poloxamer degradation, contributing to formulation development in the pharmaceutical field.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Marcus Klee (Marcus Klee, Regional Marketing Manager HPLC, Thermo Fisher Scientific)</p>
</blockquote>
<blockquote>
<p>Graduated in Chemical Engineering from Hochschule Fresenius in 2000, specialized in Marketing &amp; Sales. Since then, held various marketing roles since 2000, currently serving as the Regional Marketing Manager EMEA for HPLC</p>
</blockquote>


Thermo Scientific: Advancing analytical techniques in monoclonal antibody formulations

Advancing analytical techniques in monoclonal antibody formulations

Explore cutting-edge methodology for online protein removal and characterization of polysorbates and poloxamer in monoclonal antibody formulations.

Analytical techniques in monoclonal antibody formulations

<p><strong>Advances in monoclonal antibody (mAb) engineering have led to additional challenges in the purification of these modalities, including high levels of aggregates. Ion exchange chromatography is often not sufficient to achieve purity targets meaning hydrophobic interaction chromatography (HIC) and mixed-mode resins are needed as orthogonal tools. These technologies can be implemented in flow-through mode allowing for high loadings and fast processing times, making them well-suited for large-scale implementation.</strong></p>
<p>This webinar highlights three case studies that illustrate how novel chromatographic tools can improve the purification process for complex therapeutics by reducing the levels of aggregates and host cell proteins (HCPs) and effectively clearing retroviruses.</p>
<p><strong>Attend this webinar to explore case studies on:</strong></p>
<ul>
<li>Effective aggregate removal and viral clearance via HIC flow-through unit operation</li>
<li>High molecular weight reduction and viral clearance for a novel, pre-conjugated mAb for ADC production</li>
<li>Simultaneous HCP reduction and aggregate removal with mixed-mode chromatography for a high aggregate mAb feedstream</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Robert Stairs (Field Applications Staff Scientist, Thermo Fisher Scientific)</p>
</blockquote>


Technology Networks: Advances in Flow-Through Technology To Enhance mAb Polishing

Advances in Flow-Through Technology To Enhance mAb Polishing

Case studies that illustrate how novel chromatographic tools can improve the purification process for complex therapeutics.

Flow-Through Technology To Enhance mAb Polishing

<p><strong>Overview:</strong></p>
<p><strong>Nitrosamines – the current state of play</strong></p>
<ul>
<li>Background.</li>
<li>Potential Sources of Nitrosamines.</li>
<li>Practical Challenges for N-Nitrosamine Analysis.</li>
<li>Sample Preparation Approach for Analysis of N-Nitrosamines in Drug Products.</li>
</ul>
<p><strong>Key Learning:</strong></p>
<ul>
<li>A New SPE Method for for Simultaneous Quantification of 13 N-Nitrosamines in Various Drug Products</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Heiko Behr (Senior Business Development Manager, Phenomenex)</p>
</blockquote>


Phenomenex: New SPE Method for Simultaneous Quantification of 13 N-Nitrosamines in Various Drug Products

New SPE Method for Simultaneous Quantification of 13 N-Nitrosamines in Various Drug Products

Sample Preparation Approach for Analysis of N-Nitrosamines in Drug Products.

New SPE Method for 13 N-Nitrosamines in Drug Products

<h4><strong>Automating Laboratory Workflows with Andrew+ and OneLab Software: A Virtual Demonstration</strong></h4>
<p>We are excited to invite you to our upcoming webinar series on how to easily bring automation to your lab and transform your current workflows.</p>
<h4><strong>EPISODE 1 - Biomolecules Workflows</strong></h4>
<p>In this webinar, we’re excited to showcase Andrew+, an advanced pipetting robot, and OneLab, a workflow design execution software.</p>
<p>Both are game-changers in pharmaceutical workflows that utilize automation to streamline everything from drug discovery to quality control, including complex workflows such as released glycan analysis, peptide mapping, and amino acid analysis.</p>
<p><strong>In this episode, you will:</strong></p>
<ul>
<li>Hear directly from one of our Andrew+ users in the biotech industry, who kindly agreed to make a short video interview about their experience with Andrew+ and OneLab for oligonucleotide analysis.</li>
<li>Take a tour of the user-friendly OneLab Software, showing how Andrew+ can be intuitively guided to carry out complex tasks, reducing manual labour and minimizing errors.</li>
</ul>
<p>For example, Andrew+ and OneLab can automate sample preparation protocols for therapeutic oligonucleotide (ONT) bioanalysis that can be challenging, requiring extensive method development. Some workflows, like liquid-liquid extraction (LLE) can be difficult to automate, while others may use detergent-based reagents, requiring extensive washing and sample preparation to make it LC-MS compatible.</p>
<p>We’ll also talk about data management capabilities, highlighting how OneLab ensures data integrity and helps meet regulatory standards.</p>
<p><strong>So, come join us for this demonstration!</strong></p>
<p><em><strong>Not sure to be able to attend live sessions?</strong></em></p>
<p><em>Register today and watch the webinars on-demand</em></p>
<h4><strong>Other episodes rfom the series:</strong></h4>
<p><strong>Transform Your Daily Lab Operations with Automation - Episode 2: Pharma workflows</strong></p>
<ul>
<li>Tuesday, July 09, 2024, 11:00 AM CEST</li>
</ul>
<p><strong>Transform Your Daily Lab Operations with Automation - Episode 3: Food and Environmental Workflows</strong></p>
<ul>
<li>Thursday, September 19, 2024, 11:00 AM CEST</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Loïc Roux (Founder/CTO, OligoTune Ltd.)</p>
</blockquote>
<blockquote>
<p>Loïc Roux, PhD, Founder/CTO of OligoTune Ltd, is an accomplished large molecule scientist with a focus on nucleotide therapeutic approaches. He has worked across the globe in both healthcare and private medicine, where most recently he has partnered in the UK with Waters to set up private and public laboratories using our equipment and automation.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Philip Quinn (Senior Applications Specialist Chemistry Europe, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Phil started his career at Waters in 2018 as an Application Support Specialist in the Chemistry Consumables group for the UK. In his current role he is responsible for commercial pre and post-sale support for Chemistry Consumables and Automation across Europe. Prior to joining Waters, Phil worked at large Pharma organisations over 10+ years and gained extensive experience in Analytical Development and QC environments, including the Development, Optimisation, Validation and Transfer of LC methodologies.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Jasper Eaton (Senior Product Marketing Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Jasper has a Bachelor’s degree in General Biology and obtained a Masters Degree in Molecular and Cellular Biology from the University of Grenoble-Alpes. He was previously the EU Sales Manager at Andrew Alliance since 2017, and is now a Senior Product Marketing Manager at Waters Corporation. His experience has made him well versed in the challenges faced by customers looking to automate their lab workflows.</p>
</blockquote>


Waters Corporation: Transform Your Daily Lab Operations with Automation - Episode 1: Biomolecules workflows

Transform Your Daily Lab Operations with Automation - Episode 1: Biomolecules workflows

In this webinar, we’re excited to showcase Andrew+, an advanced pipetting robot, and OneLab, a workflow design execution software.