<div><p><strong>Protein purification is a critical step in biopharmaceutical research, proteomics, and industrial biotechnology. It involves isolating a specific protein from complex biological mixtures while preserving its native structure and activity. Traditional methods such as precipitation or dialysis lack the precision required for high-purity protein isolation, especially when dealing with therapeutic targets or complex protein assemblies.</strong></p><p>High-performance liquid chromatography (HPLC) protein purification has transformed this field by providing high resolution, reproducibility, and speed, making it indispensable for both analytical and preparative workflows.</p></div><div><div><div><div><h3><strong>What is HPLC Protein Purification?</strong></h3><p>HPLC purification is based on the principle of selective interactions between proteins and the stationary phase under high-pressure liquid flow. The separation relies on differences in size, charge, hydrophobicity, or affinity of proteins toward the stationary phase material.</p><p>Unlike conventional low-pressure methods such as gel filtration or affinity batch purification, HPLC purification operates under high pressure (up to 6000 psi), enabling faster separation with enhanced resolution and quantitative recovery. It also offers precise control over parameters like gradient composition, pH, and temperature, minimizing variability between runs.</p><p>In contrast to traditional column chromatography, HPLC uses smaller stationary phase particles (3–10 µm), resulting in increased surface area and sharper <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/how-to-reduce-peak-tailing-in-hplc"><strong>peak shapes</strong></a>, which are vital for protein purity assessment and scale-up purification.</p></div></div></div></div><div><div><div><div><h3><strong>Types of HPLC Used for Protein Purification</strong></h3></div></div></div></div><div><p>Different HPLC modes can be employed depending on the physicochemical properties of the target protein. Each method offers unique advantages in terms of selectivity and recovery.</p><h4><strong>Reversed-Phase HPLC (RP-HPLC)</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase"><strong>Reversed-Phase HPLC</strong></a> separates proteins based on hydrophobic interactions between the analyte and the stationary phase, typically C4, C8, or C18 silica-based columns. Proteins are eluted using gradients of organic solvents (usually acetonitrile or methanol) in the presence of volatile acids like formic or trifluoroacetic acid (TFA).</p><p>This method provides exceptional resolution and is commonly used for protein fragment purification, and analysis of recombinant proteins. For example, reversed-phase HPLC is routinely employed in peptide therapeutic workflows, such as the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an59280424_w_fc6c3ac6f0.pdf"><strong> Tirzepatide Preparation and Purification </strong></a>process, where purity and recovery are critical to product efficacy. However, due to exposure to organic solvents, RP-HPLC may cause partial denaturation, limiting its use for native proteins.</p><h4><strong>Ion Exchange HPLC (IEX-HPLC)</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec"><strong>IEX-HPLC</strong></a> separates proteins based on net surface charge. Cation exchange (with negatively charged stationary phases) binds positively charged proteins, while anion exchange does the reverse.</p><p>By gradually increasing salt concentration or changing the mobile phase pH, proteins are eluted according to their isoelectric points (pI).By gradually increasing salt concentration or changing the mobile phase pH, proteins are eluted according to their isoelectric points (pI).</p><p>This approach is highly suitable for native purification, protein isoform analysis, and charge variant characterization essential steps in therapeutic protein production.</p><h4><strong>Size Exclusion Chromatography (SEC-HPLC)</strong></h4><p>SEC separates molecules based on their hydrodynamic radius. Larger proteins elute first, as they are excluded from the pores of the stationary phase, while smaller molecules penetrate and elute later.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec"><strong>SEC-HPLC</strong></a> is non-denaturing and ideal for determining protein molecular weight, oligomeric state, and aggregate content. It is frequently used as a final polishing step following ion exchange or affinity chromatography.</p><h4><strong>Affinity HPLC</strong></h4><p>Affinity-based HPLC leverages specific biological interactions between the target protein and a ligand immobilized on the stationary phase (e.g., antibodies, metal ions, or tags such as His-tag or GST).</p><p>When integrated into an HPLC system, affinity chromatography provides high selectivity and purity (&gt;95%) in a single step, often used in recombinant protein purification and biomarker capture applications.</p></div><div><div><div><div><h3><strong>Benefits of Using HPLC for Protein Purification</strong></h3><p>HPLC-based protein purification combines the analytical precision of chromatography with the preparative capability needed for research and biomanufacturing. The main advantages include:</p><h5><strong>Benefit &nbsp;/ &nbsp;Description</strong></h5><p><strong>High Resolution and Selectivity:</strong></p><ul><li>Smaller stationary phase particles and optimized flow dynamics allow HPLC to resolve proteins differing by minimal physicochemical properties. This precision is vital for distinguishing isoforms, mutants, and post-translationally modified variants.</li></ul><p><strong>Accuracy and Reproducibility</strong></p><ul><li>Automated systems maintain stable flow rates, gradient control, and detection sensitivity, ensuring reproducible results essential for regulated biopharmaceutical workflows.</li></ul><p><strong>Speed and Efficiency</strong></p><ul><li>Modern UHPLC systems equipped with fully porous or core–shell particles achieve faster separations without sacrificing resolution, reducing run times by up to 50% compared to classical methods.</li></ul><p><strong>Compatibility and Scalability</strong></p><ul><li>HPLC systems are highly adaptable, supporting both analytical-scale characterization and preparative-scale purification. Methods developed at microgram scale can be seamlessly transferred to milligram or gram scales used in process development.</li></ul><div><div><div><div><h3><strong>Factors Affecting the Efficacy of Protein Purification by HPLC</strong></h3></div></div></div></div><div><p>Optimizing HPLC for protein purification requires balancing purity, recovery, and throughput. The following parameters significantly influence results:</p><ul><li><strong>Purification goal:</strong> Define whether the priority is yield, purity, or throughput. Analytical separations prioritize resolution, while preparative runs emphasize recovery and process speed.</li><li><strong>Protein solubility and stability:</strong> Unstable or aggregation-prone proteins may require low temperatures or additives (e.g., glycerol, mild detergents).</li><li><strong>Column chemistry and particle size:</strong> Select appropriate stationary phase chemistry (silica, polymeric, or mixed-mode) and particle size to suit molecular weight and hydrophobicity.</li><li><strong>Mobile phase pH and ionic strength:</strong> pH should be optimized near but not at the protein’s pI; salt gradients influence both resolution and elution strength.</li><li><strong>Flow rate and gradient profile:</strong> Steeper gradients shorten run time but may compromise separation quality.</li><li><strong>Purified protein final form:</strong> Consider buffer composition and concentration for storage or downstream assays to maintain bioactivity.</li></ul></div><div><div><div><div><h3><strong>Best Practices for Protein Purification by HPLC</strong></h3></div></div></div></div><div><p>Following good chromatographic practices ensures consistent, high-quality results:</p><ul><li><strong>Column conditioning and storage:</strong> Always equilibrate new <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=48&amp;manufacturerItems=50"><strong>HPLC columns</strong></a> with 10 column volumes of buffer; store in appropriate solvent containing preservatives.</li><li><strong>Avoid protein precipitation:</strong> Maintain appropriate ionic strength and, when native state is desired, avoid exposure to denaturing solvents or high organic content.</li><li><strong>Prevent sample loss:</strong> Use low-protein-binding tubing and vials; minimize dead volume in the system.</li><li><strong>Troubleshooting:</strong><ul><li>Peak fronting - may indicate column overloading.</li><li>Peak broadening – may indicate column channeling.</li><li>Carryover – reduce by extending wash cycles or using stronger regeneration solvents.</li><li>Clogging – prefilter samples (0.22 µm) and periodically flush the system.</li></ul></li></ul></div></div></div></div></div>

Protein Purification by HPLC: Methods, Benefits, and Best Practices

<p><strong>Scientists from CEITEC Brno University of Technology&nbsp;have succeeded in a highly competitive international selection process and secured a prestigious Human Frontier Science Program (HFSP) grant, which supports groundbreaking fundamental research. The Brno-based team led by Eric Daniel Glowacki will contribute a unique technology to an international project that enables precise control of oxygen levels in tissues. Changes in oxygen availability may play a crucial role in determining how cells specialize during early embryonic development.</strong></p><p>The project, entitled “Beyond Theory: Regulation of Mammalian Embryo Patterning by Electrochemical Gradients,” is being carried out by CEITEC BUT in collaboration with biologists from the <strong>Max Planck Institute of Molecular Cell Biology and Genetics</strong> and <strong>Yale University</strong>. The Brno team’s role is central to the project: providing technology that enables the deliberate creation of regions with different oxygen concentrations in tissues and the observation of how cells respond to these changes.</p><p><i>“Our research group specializes in electrochemistry, particularly in studying how electric current affects tissue oxygenation and the resulting chemical changes. To perform certain types of experiments, we developed our own integrated system of needle probes, electrodes, and software, which allows us to influence and control the amount of oxygen present in tissues through which current is passed,” </i>explains team leader <strong>Eric Daniel Glowacki</strong>. Using these tools, researchers can create regions within samples where oxygen is scarce or even completely absent. Changes in oxygen levels are fundamental to biology because they affect cellular metabolism and, consequently, cell behaviour. In some types of research, such precise environmental control is essential.</p><p><i>“Our system is not universal; we tailor it to each application. In the future, however, we aim to expand its variants so that it can be used across the widest possible range of research fields,”</i> adds Eric.</p><p>Scientists at the Max Planck Institute have already used CEITEC’s technology in previous studies of early embryonic development. They investigated how the metabolic activity of stem cells – undifferentiated cells capable of self-renewal and transformation into specialized cells such as muscle, nerve, or blood cells – affects both their own development and that of neighbouring cells.</p><p>The newly funded HFSP project builds on this work. Its goal is to revisit the nearly century-old theory&nbsp;proposed by the American zoologist Charles Manning Child, which explains how stem cells in multicellular organisms gradually develop into mature cells with specific functions and structures. The three-year project, supported by a grant of<strong> CZK 7.5 million </strong>for each partner, will test a new hypothesis in mouse embryos directly in the uterus: that the specific transformation of cells depends not only on oxygen depletion itself but also on the cell’s location within the tissue and the timing of the oxygen decrease.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Technologie_vedcu_z_CEITEC_pomuze_odhalit_jak_kyslik_ridi_vyvoj_embryi_2_1ea86be296.jpeg" alt="CEITEC/ Lukáš Havlák: CEITEC Technology Will Help Reveal How Oxygen Controls Embryo Development: HFSP technological setup at CEITEC BUT." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Technologie_vedcu_z_CEITEC_pomuze_odhalit_jak_kyslik_ridi_vyvoj_embryi_2_1ea86be296.jpeg 235w," sizes="100vw" width="1600" height="1067"></p><p>A better understanding of the mechanisms that govern early embryonic development could, in the future, contribute to advances in regenerative medicine, research into developmental disorders, and the more effective use of stem cells in biomedicine.</p><p><i>“The success of our project is both a great honour and a significant responsibility. HFSP is a unique programme dedicated to fundamental research, where investigators seek answers to the most basic and still unresolved questions in biology,”</i> says Eric. The programme is extremely competitive, with only two to three percent of the thousands of submitted projects receiving funding. Requirements include international collaboration across continents and a genuinely innovative approach.</p><p><i>“Through this, we have become part of a community of world-class scientists from around the globe. Although the results of this kind of research are not immediately applicable, their long-term benefit to society is immense,” </i>Eric concludes.</p><blockquote><h4><strong>About HFSP</strong></h4><p>The Human Frontier Science Program Organization (HFSPO) was established in France in 1989, although the idea of creating such an international community dedicated to fundamental research originated several years earlier in Japan. The programme funds cutting-edge research in life sciences, particularly in biology, physics, chemistry, mathematics, and computer science, and supports innovative interdisciplinary projects that would otherwise be difficult to finance.</p><p>HFSPO receives funding from governments and institutions, including those of France, Japan, Switzerland, South Korea, the United States, the European Union, Singapore, New Zealand, and the United Kingdom. Since the programme’s launch in 1989, 31 HFSP-supported scientists have gone on to receive Nobel Prizes. The most recent awardees, in 2024, were David Baker, who received the Nobel Prize in Chemistry for computational protein design, and Gary Ruvkun, who received the Nobel Prize in Physiology or Medicine for the discovery of microRNA.</p></blockquote>

CEITEC Technology Will Help Reveal How Oxygen Controls Embryo Development

<p><strong>🎤Rajas Poorna and Charles Anderson</strong></p><h4><strong>What if you could dry biofluid samples in the field—without a vacuum system, freeze dryer, or full lab setup?</strong></h4><p>In this episode of Concentrating on Chromatography, I sit down with Rajas Poorna and Charles Anderson to explore their work on a frugal, field-deployable alternative to traditional lyophilization through the Evapinator project.</p><p>Originally inspired by the principles of frugal science, their approach rethinks how we stabilize and prepare biofluid samples like blood and plasma—especially in environments where traditional lab infrastructure simply isn’t available.</p><h4><strong>What This Episode Covers:</strong></h4><h5><strong>A Frugal Take on Lyophilization</strong></h5><p>Can you replicate the outcome of a lyophilizer without the complexity?<br>Why traditional freeze drying is often too expensive and impractical for field use<br>How simplifying evaporation could unlock entirely new workflows</p><h5><strong>Drying Biofluids in the Field</strong></h5><p>The real-world challenge of handling blood and other biofluids outside the lab<br>Why sample stability during transport is such a critical bottleneck<br>How a portable system could enable on-site sample prep in remote or resource-limited settings</p><h5><strong>Rethinking Evaporation (No Vacuum Required)</strong></h5><p>The core idea behind the Evapinator—and how it challenges conventional assumptions<br>The role of airflow, heat, and design in drying sensitive samples<br>Where this approach fits relative to traditional evaporation and lyophilization techniques</p><h5><strong>Early Results &amp; Practical Insights</strong></h5><p>What’s working so far—and where limitations still exist<br>The types of applications where this approach shows the most promise<br>What still needs to be validated in upcoming peer-reviewed work</p><h4><strong>Why This Matters</strong></h4><p>From clinical research in remote regions to decentralized diagnostics, the ability to dry and preserve biofluid samples in the field could fundamentally change how and where analysis happens.</p><p>This conversation challenges a core assumption in analytical science:</p><p>Do you really need a full lyophilizer—or even a vacuum—to stabilize biofluid samples?</p><h3><strong>Video Transcription</strong></h3><p>The interview opens with a welcome to an episode of <i>Concentrating on Chromatography</i>. The host introduces guests <strong>Rajas Poorna</strong> and <strong>Charles Anderson</strong> and asks about their scientific backgrounds and how they became involved in solving evaporation challenges.</p><h4><strong>Scientific background and project origin</strong></h4><p>Rajas explains that he is trained as a physicist and is now pursuing a PhD in bioengineering. His lab focuses on building low-cost scientific equipment to make science more accessible, especially for schools and low-resource settings. This work is part of a broader “frugal science” approach, where students and researchers develop affordable versions of instruments that are normally expensive.</p><p>The original challenge came from synthetic biology education. High school teams working on synthetic biology projects needed access to equipment such as lyophilizers, but conventional systems can cost tens of thousands of dollars. The team therefore began exploring whether a more affordable lyophilizer-like system could be built for education and field use.</p><p>Charles describes joining the project early in his undergraduate studies. He was drawn to it because it had a clear practical purpose and seemed like something that could become useful outside the laboratory rather than remaining only a research prototype.</p><h4><strong>Why evaporation became the key challenge</strong></h4><p>The discussion turns to the practical limitations of traditional evaporation and drying systems. Rajas explains that during a collaboration in Thailand, researchers working on Raman spectroscopy for tuberculosis diagnostics needed to dry blood-derived samples before transporting them to a central laboratory. They used a centrifugal vacuum evaporator, but the system was slow, expensive, and difficult to service when it broke down.</p><p>This experience led to a central question: could sample drying be achieved without a vacuum? Rajas notes that everyday drying processes, such as drying clothes, do not require vacuum. This insight opened the path toward a simpler, smaller, lower-cost and potentially battery-powered drying system.</p><h4><strong>How Rajas and Charles began collaborating</strong></h4><p>Charles explains that he met the lab’s principal investigator while discussing a different project. He was then invited to work on what would later become the Evaponator. At the beginning, the project was still small and exploratory.</p><p>Rajas adds that Charles joined at a stage when the team was trying to understand the physics and engineering behind vacuum-free drying. Within a few months, they developed a prototype costing under 100 USD that could dry samples much faster than the conventional vacuum evaporator used by collaborators.</p><h4><strong>Main limitations of conventional drying systems</strong></h4><p>The interview identifies several major limitations of existing evaporation technologies:</p><ul><li>High cost</li><li>Limited reliability in remote or low-resource settings</li><li>Long drying times</li><li>Poor field portability</li><li>Challenges with sterility and cross-contamination</li></ul><p>Charles emphasizes that freeze drying can take 12–24 hours, which is impractical for field use. Rajas adds that centrifugal vacuum evaporators are not necessarily sterile and may pose a risk of cross-sample contamination. Together, these challenges shaped the requirements for the Evaponator.</p><h4><strong>What the Evaponator is</strong></h4><p>Charles introduces the Evaponator as a low-cost, portable drying system designed to dry small numbers of samples quickly without a vacuum chamber. The name was inspired by an episode of <i>Phineas and Ferb</i>.</p><p>The system is based on the same principles as drying clothes: low humidity, gentle heating and airflow. Instead of using vacuum, the Evaponator generates dry air, directs it over the sample, removes moisture through a cold trap and repeats the process in a closed or semi-closed loop.</p><p>The current design is aimed at drying approximately four 1 ml samples, which fits the intended use case of field diagnostics or low-throughput sample collection in remote settings.</p><h4><strong>How the system works</strong></h4><p>Rajas explains the operating principle in more detail. Moist air is passed through a cooled metal block using a thermoelectric cooler. As the air cools, moisture condenses inside the cold trap, producing dry air. This dry air is then directed over warmed samples, helping water evaporate while keeping the sample temperature moderate.</p><p>The system can operate in a loop to improve efficiency. HEPA filtration and aerosol filtration help prevent contamination. The team also developed an ozone-based sterilization approach, allowing the device to sterilize itself using ozone generated from air rather than relying on consumables such as ethanol or peroxide.</p><p>To prevent sample-to-sample contamination, the design uses separate airflow paths for individual samples, which merge only later in the system.</p><h4><strong>Design assumptions and engineering constraints</strong></h4><p>A key design assumption is that not every drying process needs sublimation or deep vacuum. For the biofluid applications targeted by the Evaponator, the team works around 40 °C, a temperature chosen because it is close to fever temperature and should be mild enough for many biological samples.</p><p>The engineering constraints included:</p><ul><li>Use of widely available components</li><li>No specialized machining</li><li>Bill of materials under 100 USD</li><li>Low power consumption</li><li>Portability</li><li>Simple assembly</li><li>Robust airflow without splashing or sample loss</li></ul><p>Charles notes that the team intentionally selected accessible parts, including components similar to those used in computer cooling systems, vacuum-cleaner-style HEPA filters and disposable pipette tips for directing airflow.</p><h4><strong>Sample types and solvents</strong></h4><p>The prototype is currently focused mainly on aqueous biological samples. Rajas explains that using organic solvents such as ethanol would introduce ignition risks with the current architecture, so the early work is focused on water-based samples and biofluids.</p><p>Charles adds that the system is intended primarily for blood-derived fluids, urine, saliva and similar samples where the target analytes can remain stable after drying and rehydration.</p><h4><strong>Potential analytical applications</strong></h4><p>The Evaponator is being developed for field sample preparation rather than as a replacement for every laboratory evaporator. The main idea is to dry samples near the point of collection, transport them more easily and then rehydrate them before analysis.</p><p>Potential use cases include:</p><ul><li>Field diagnostics</li><li>Biofluid sample stabilization</li><li>Remote sample collection</li><li>Clinical or public-health testing in low-resource settings</li><li>Applications where dried plasma, serum, urine or saliva can later be analyzed</li><li>Situations where conventional dried blood spot methods lack enough sample volume</li></ul><p>Rajas notes that red blood cells may not be suitable for drying directly because they can rupture, so serum or plasma is expected to be more appropriate. He also points out that oxygen exposure during forced-air drying still needs further testing for oxygen-sensitive analytes.</p><h4><strong>Current development status</strong></h4><p>Charles explains that the project is approaching important validation milestones, especially around batch-to-batch and sample-to-sample sterility. The ozone cleaning system appears promising because it may reduce the need for cleaning consumables, aside from inexpensive items such as pipette tips and occasional filter replacement.</p><p>He also mentions tests with trehalose-based solutions and collaboration with a Georgia Tech startup working on room-temperature-stable mRNA vaccines. In that context, the Evaponator was able to dry samples in about 30 minutes, compared with 12–24 hours using previous drying approaches.</p><h4><strong>Commercialization and future direction</strong></h4><p>Rajas explains that the team is exploring commercialization and has participated in a startup accelerator. They are investigating where the technology could have the strongest product-market fit, with particular interest from cost-sensitive and geographically dispersed healthcare settings, including India.</p><p>The goal is to move beyond a lab prototype toward a manufacturable, user-friendly device with a straightforward interface and reliable sealing, sterilization and operation.</p><h4><strong>Closing remarks</strong></h4><p>The interview concludes with the host thanking Rajas Poorna and Charles Anderson for sharing the technical and practical challenges behind the Evaponator. Both guests emphasize that the conversation was a valuable opportunity to explain the project in depth and reflect on its progress.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Frugal Science Meets Sample Prep: A Field-Ready Lyophilizer for Biofluids

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Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

ALS Czech Republic

Agilent Technologies

SelectScience

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Waters Corporation

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<p><strong>Sample Preparation - Tips &amp; Tricks to make it simple</strong></p>
<p><strong>What will you learn?</strong></p>
<ul>
<li>Advantage of water-wettable Sorbents</li>
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<p>Back to Basics - Sample Preparation: Theory and Overview</p>
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<p>Back to Basics - Sample Preparation: Tips &amp; Tricks to make it simple</p>
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Sample Preparation: Tips & Tricks to make it simple

The first episode will explain the advantage of water-wettable sorbents and path to successful SPE.

Sample Preparation: Theory and Overview

<h4><strong>LC-MS/MS fundamentals webinar series 2.0</strong></h4>
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<p>July 16, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3856"><strong>Automating data processing</strong></a></p>
<p>Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.</p>
<p>July 30, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3857"><strong>Excel strategies for mass spectrometry data analysis</strong></a><br/>Take an in-depth look at when and how to use Excel to increase your productivity.</p>


SCIEX: LC-MS/MS fundamentals webinar series 2.0

Mastering chromatography

Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series.

<p><strong>This seminar addresses cutting-edge strategies targeting multiple stages of the Plasmodium parasite lifecycle in mosquitoes and how conjugating poorly immunogenic molecules with potent carriers can revolutionize malaria vaccine development.</strong></p>
<p>Malaria eradication efforts rely on innovative approaches like transmission-blocking vaccines (TBVs)</p>
<p>This study from <strong>Dr. David Narum</strong> at the NIH, examines human antibodies produced in response to vaccine candidates and how tailored development of vaccines are critical for effectively combating diseases like malaria.</p>


Waters Corporation: Application of SEC-MALS-QELS in the Development of Recombinant Protein-Based Malaria Vaccinesased Malaria Vaccines

Application of SEC-MALS-QELS in the Development of Recombinant Protein-Based Malaria Vaccines

This seminar addresses cutting-edge strategies targeting multiple stages of the Plasmodium parasite lifecycle in mosquitoes.

SEC-MALS-QELS for Recombinant Protein-Based Malaria Vaccines

<p><strong>Semiconducting materials are widely studied for use in solar energy and photocatalytic applications, among other areas of interest. For applications which involve the use of light, it is important to understand the energy needed to promote electrons from the valence band to the conduction band. This band gap energy can often be on par with the energy of photons in the UV-Visible region.</strong></p>
<p>As such, UV-Visible spectroscopic techniques can be of use when assessing the band structure of semiconducting materials. Tauc plots, which are derived from the UV-Visible spectrum, provides the needed information to estimate the bandgap energy. In conjunction with computation and experimental results, the information gathered from the Tauc plot can aid in assessing the relative position of the valence and conduction bands.</p>
<p>In this webinar, the use of UV-Visible reflectance and transmittance measurements for band gap analysis will be discussed in detail. Additionally, considerations needed when analyzing semiconducting samples with these methods with be outlined.</p>
<h5>Key Learning Objectives:</h5>
<ul>
<li><p><strong>Gain knowledge about the theory and application of Tauc plot analyses for both reflectance and transmittance measurements.</strong></p>
</li>
<li><p><strong>Learn what sample properties and environmental factors can influence the measured spectra of a semiconducting sample.</strong></p>
</li>
<li><p><strong>Understand the considerations needed to perform accurate UV-Visible measurements of films or colloidal solutions.</strong></p>
</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Research Scientist</li>
<li>Materials Scientist/Engineer</li>
<li>Semiconductor Engineer</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Jennifer Empey-Kohl, PhD (Applications Scientist, Thermo Fisher Scientific)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)</p>
</blockquote>


C&EN: Analysis of Semiconductors through UV-Visible Techniques: Band Gap Studies

Analysis of Semiconductors through UV-Visible Techniques: Band Gap Studies

In this webinar, the use of UV-Visible reflectance and transmittance measurements for band gap analysis will be discussed in detail

Semiconductors: UV-Visible Techniques: Band Gap Studies

<p><strong>Development and production of IVT mRNA vaccines requires measurements of Critical Quality Attributes (CQAs), such as identity, purity, quality, and quantity. Orthogonal methods developed for such purposes are outlined in the USP guidelines for mRNA vaccines.</strong></p>
<p>Agilent Technologies provides many analytical solutions that are used by leading pharmaceutical companies in early-stage drug development through production quality control (QC) and final product release. These systems help users save time and money with optimized workflows, streamlined processes, and fast time to results.</p>
<p>Whitney Pike, M.Sc, Application Scientist and Dr. Sonja Schneider, Manager Application Development, Agilent Technologies, will discuss how the automated electrophoresis and LC/MS systems solutions from Agilent provide trusted answers for IVT mRNA workflows at many steps, from QC of the initial plasmid DNA, throughout the IVT process, and the finished mRNA drug product.</p>
<h5>Key learning objectives</h5>
<ul>
<li><p><strong>Explore QC throughout the entire IVT mRNA workflow, including data from various nucleic acids and LNP analyses</strong></p>
</li>
<li><p><strong>Discover how Agilent instruments/technologies, such as the Fragment Analyzer and LC/MS systems, can be used for different CQAs</strong></p>
</li>
<li><p><strong>Learn how Agilent is working together with its partners to provide trusted answers for all aspects of the IVT mRNA workflow</strong></p>
</li>
</ul>
<h5>Who should attend?</h5>
<ul>
<li>Researchers, lab managers, lab directors, QA/QC managers performing IVT mRNA analysis in biotherapeutics research and production</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Whitney Pike, MA (Application Scientist, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Whitney Pike joined Agilent in 2016 and is an application scientist with a focus on Agilent’s automated electrophoresis instruments. She played a pivotal role in the release of the Femto Pulse system as a scientist on the product development team. She completed her master&#39;s degree in Biology, with an emphasis on cellular, molecular, and developmental biology, before working at a core facility at Iowa State University, responsible for performing next-generation sequencing studies.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Sonja Schneider (Manager Application Development, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Dr. Sonja Schneider joined Agilent in 2011 as an Application Scientist for biopharma applications. She has been leading the Application Development Team since 2016 and still works in the lab on up-to-date biopharma applications. She completed her diploma degree in Biology and her phD in Biochemistry before joining Agilent.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Will Thompson (Editorial Team, SelectScience)</p>
</blockquote>
<blockquote>
<p>Will studied Biology at the University of Bristol, UK, and as a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.</p>
</blockquote>


SelectScience: From start to finish: How Agilent Technologies can provide trusted answers for producing IVT mRNA-based vaccines

From start to finish: How Agilent Technologies can provide trusted answers for producing IVT mRNA-based vaccines

How the automated electrophoresis and LC/MS systems solutions from Agilent provide trusted answers for IVT mRNA workflows at many steps

Agilent Technologies in producing IVT mRNA-based vaccines

<p><strong>This comprehensive seminar explores the use of High-Performance Thin-Layer Chromatography (HPTLC) in forensic science. Participants will gain in-depth insights into cutting-edge techniques for the analysis and identification of explosives, chemical warfare agents, drugs of abuse, and cannabis. Through practical examples and case studies, the seminar will demonstrate how HPTLC is employed for precise control and limit testing. This seminar is designed for forensic analysis professionals looking to enhance their expertise and improve the effectiveness of their investigations.</strong></p>
<p><strong>Key learning objectives</strong></p>
<ul>
<li>Find out how HPTLC is important in forensics.</li>
<li>Learn how HPTLC methods were developed for chemical warfare and explosives.</li>
<li>Discover pragmatic approaches using HPTLC</li>
</ul>
<p><strong>Who should attend?</strong></p>
<p>This webinar would be beneficial for professionals and researchers working in forensic science, including forensic analysts, laboratory technicians, law enforcement personnel, forensic scientists, chemists, and anyone involved in the analysis of forensic evidence. Additionally, individuals interested in the application of chromatography techniques in forensic investigations would find this webinar valuable.</p>
<p><strong>Certificate of attendance</strong></p>
<p>All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</p>
<blockquote>
<p><strong>Presenter:</strong> Dr. Tiên Do (Chief Scientific Officer, CAMAG)</p>
</blockquote>
<blockquote>
<p>Tiên Do has been with CAMAG since 2014, rising to the role of Chief Scientific Officer in January 2024. In this capacity, she crafts and implements the organization&#39;s scientific vision, spearheading research and development efforts to propel scientific objectives forward. Additionally, she cultivates partnerships with academic and industry leaders to drive innovative collaborations. Prior to her current role, Tiên served as Head of Laboratory and Deputy Head of Laboratory.</p>
</blockquote>
<blockquote>
<p>Tiên earned her PhD from the University of Nice, France, for her dissertation titled &quot;Evaluation of HPTLC in the analysis of natural extracts.&quot; She shares her expertise as a lecturer at the Leiden University metabolomics workshop and has contributed 14 articles on HPTLC to esteemed peer-reviewed journals.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Raphael Vizzini (Area Sales Director EMEA &amp; CIS, CAMAG)</p>
</blockquote>
<blockquote>
<p>Raphael Vizzini is an experienced Area Sales Director for EMEA and CIS at CAMAG, a role he has held since 2008. With a solid background in organic chemistry, specializing in small molecules, Raphael transitioned from science to sales, bringing a deep understanding of technical processes to his strategic sales initiatives. At CAMAG, he has successfully led and initiated numerous projects, particularly in the areas of explosives and chemical warfare. His expertise in navigating complex market dynamics and developing strategic partnerships has significantly contributed to the expansion and success of CAMAG’s operations in these regions.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Carrie Haslam (Associate Editor, SelectScience)</p>
</blockquote>
<blockquote>
<p>Dr. Carrie Haslam is an Associate Editor at SelectScience, playing a key role in content production and specializing in Materials Science, Alzheimer’s disease and Clinical Diagnostics.</p>
</blockquote>
<blockquote>
<p>Carrie completed a Ph.D. from The University of Plymouth, where she developed graphene-based biosensors for the early diagnosis of Alzheimer’s disease.</p>
</blockquote>


SelectScience: Unlocking Forensic Mysteries: Advanced HPTLC Techniques for Explosives and Chemical Warfare

Unlocking Forensic Mysteries: Advanced HPTLC Techniques for Explosives and Chemical Warfare

This comprehensive seminar explores the use of High-Performance Thin-Layer Chromatography (HPTLC) in forensic.

HPTLC Techniques for Explosives and Chemical Warfare

<p><strong>Join us for an engaging webinar on high throughput analytical data management featuring Holly Douglas, Senior Research Scientist from AstraZeneca.Discover how integrating the Reaction Optimization software solution is revolutionizing drug discovery by streamlining workflows and enhancing data quality. Register now for valuable insights into the future of high throughput experimentation.</strong></p>
<p>We are excited to invite you to an insightful webinar titled “Accelerating High Throughput Experimentation Workflows using Automated Data Processing”, featuring Holly Douglas, Senior Research Scientist at AstraZeneca.</p>
<p>In this webinar, Holly will showcase how the integration of the Reaction Optimization software solution is revolutionizing synthetic workflow in drug discovery.</p>
<p>Discover how this innovative approach eliminates data processing bottlenecks, enhances data quality, and sets new standards for efficiency and reliability in high throughput experimentation.</p>
<p><strong>Who Should Attend:</strong></p>
<ul>
<li>Organic, medicinal, or process chemists seeking to streamline their analytical workflows and enhance efficiency.</li>
<li>Lab or project managers interested in optimizing laboratory productivity by implementing innovative software solutions.</li>
<li>Scientists dealing with large volumes of analytical data, eager to explore automation possibilities.</li>
</ul>
<p><strong>SESSION 1 Wednesday, June 5th, 2024</strong></p>
<p>4:00 pm CEST/10:00 am EDT/7:00 am PDT - <a href="https://zoom.us/webinar/register/3317162892383/WN_IplNoiMBTIixdpjcnIYcUA"><strong>Register Here</strong></a></p>
<p><strong>SESSION 2 Thursday, June 6th, 2024</strong></p>
<p>10:00 am CEST/2:30 pm IST/6:30 pm ACST - <a href="https://zoom.us/webinar/register/7617162915680/WN_RmP5j38rSfKSIX1FD2haZg"><strong>Register Here</strong></a></p>
<blockquote>
<p><strong>Presenter:</strong> Holly Douglas (Senior Research Scientist, AstraZeneca)</p>
</blockquote>
<blockquote>
<p>Holly has a broad range of experience applying analytical chemistry techniques to a variety of research projects. The majority of Holly’s experience has been in the pharmaceutical industry having worked as an analytical chemist at GSK, Novartis and the MHRA, with roles based in drug discovery, process chemistry and final product testing. More recently Holly has worked at Owlstone Medical where she developed separation and detection methods for identifying volatile biomarkers in breath samples and the Francis Crick Institute where she was involved in the LC-MS investigation of the metabolomics and drug resistance of Mycobacterium tuberculosis.  </p>
</blockquote>


Mestrelab Research: Accelerating High Throughput Experimentation Workflows using Automated Data Processing

Accelerating High Throughput Experimentation Workflows using Automated Data Processing

Integrating the Reaction Optimization software solution is revolutionizing drug discovery by streamlining workflows and enhancing data quality.

High Throughput Workflows with Automated Data Processing

<p><strong>In this webinar you will discover how extractables and leachables (E&amp;L) testing can be streamlined using acquisition modes enhanced by QTRAP technology. If you are new to E&amp;L testing or want to learn how your own workflows can be improved when using nominal mass technology then this webinar is for you.</strong></p>
<p><strong>Key topics that will be covered in this webinar include:</strong></p>
<ul>
<li>Introduction to E&amp;L analysis using nominal mass instrumentation</li>
<li>How the QTRAP can enhance E&amp;L analysis</li>
<li>The importance of libraries and how they can be implemented to improve compound identification</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Ferran Sanchez (Market Development Manager, Pharma/CRO (MS &amp; CE), EMEAI, SCIEX)</p>
</blockquote>
<blockquote>
<p>Ferran obtained his PhD in Pharmaceutical Sciences at the University of Barcelona in 2005 and has been involved with mass spectrometry ever since. He has worked for SCIEX for 18 years holding different positions in sales, marketing and business development covering the EMEAI region. During this time, Ferran has been working closely with customers in the pharmaceutical industry to deepen his understanding of the market and user needs for delivering the right solutions at the right time.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Jack Steed, Sr. (Technical Specialist, SCIEX)</p>
</blockquote>
<blockquote>
<p>Jack joined SCIEX in 2019 as a technical specialist, his main responsibilities include the creation and proliferation of technical marketing content such as application notes and presentations as well as having a strong focus on the pharma market and small molecule analysis. Prior to joining SCIEX, Jack was employed by a CRO working mainly in small molecule method development and validation, with an emphasis on LC and LC-MS analysis.</p>
</blockquote>


SCIEX: Extractables and leachables: the complete workflow using QTRAP LC-MS/MS technology

Extractables and leachables: the complete workflow using QTRAP LC-MS/MS technology

Discover how extractables and leachables (E&L) testing can be streamlined using acquisition modes enhanced by QTRAP technology.

Extractables and leachables: QTRAP LC-MS/MS workflow

<p><strong>Together with ALS Czech Republic, we bring you an exclusive webinar series offering insights into the world of per- and polyfluoroalkyl substances (PFAS). The three-part series will bring together regulatory experts from REGARTIS and environmental laboratories professionals of ALS Czech Republic to address PFAS from environmental risks to the evolving regulatory landscape and analytical challenges.</strong></p>
<p>First, we will explore their uses, environmental hazards, and restriction efforts. The second part will be focused on analytics, helping attendees understand PFAS testing in various matrices and the implications of their widespread environmental presence. Finally, the series will offer a discussion on regulatory processes, compliance strategies, and the technical aspects of PFAS analysis, such as sampling rules and possible matrices types. The core aim of the series is to provide chemical industry stakeholders with the knowledge to effectively manage PFAS compliance and analysis both.</p>
<h3><strong>Changes to regulations and restriction</strong></h3>
<ul>
<li>Mgr. Yana Tributsyna</li>
</ul>
<p>The first session by REGARTIS will look into restrictions of PFAS under consideration due to their adverse effects and persistence in the environment. We will also discuss the global emissions and presence of PFAS and outline the upcoming regulatory changes. These include a detailed look at already restricted PFAS, the REACH restriction process, and new proposals that could impact a broad array of PFAS compounds.</p>
<blockquote>
<p><strong>Presenter:</strong> Mgr. Yana Tributsyna (Regulatory Queen, REACH manager – REGARTIS)</p>
</blockquote>
<blockquote>
<p>Yana graduated from Charles University in Prague, where she studied molecular biology and genetics. She is skilled in REACH registration dossier compilation and SDS/eSDS creation, she also has experience with chemical auditing and biocidal regulations. She speaks fluently Russian, Czech, and English. She is a certified REACH manager.</p>
</blockquote>


ALS Czech Republic: PFAS: Strategic Approaches for Chemical Industry Compliance

PFAS: Strategic Approaches for Chemical Industry Compliance - Changes to regulations and restriction

Look into restrictions of PFAS under consideration due to their adverse effects and persistence in the environment.

PFAS: Changes to regulations and restriction

<p><strong>Mass spectrometry imaging (MSI) relies on the analysis of complex mixtures of analytes extracted from each location on the sample without any prior separation. Advancements in high resolution mass spectrometers have facilitated the separation of isobars, making these systems popular platforms in MSI studies. However, even with the highest achievable mass resolving power, these instruments cannot separate isomers and closely-spaced isobaric species.</strong></p><p>In contrast, multiple reaction monitoring (MRM) mode on the Ultivo triple quadropole mass spectrometer (QqQ) enables the separation of both isobaric and isomeric species. We have implemented nanospray desorption electrospray ionization (nano-DESI) MSI on a QqQ and demonstrated the spatial mapping of isobaric lipids that require a mass-resolving power of &gt;3,850,000 for separation in conventional MS1 mode.</p><p>In addition, using unique MRM transitions for each isomer, we have also successfully separated eicosanoid isomers, specifically hydroxyeicosatetraenoic acids (HETEs), in mouse kidney tissues. These byproducts of arachidonic acid oxidation play important roles in inflammation, stroke, and cancer. Nano-DESI MSI in the MRM mode is also ideally suited for isomer differentiation using online derivatization techniques.</p><p>We have demonstrated the unique capabilities of this approach for imaging of isomeric species in biological tissues that cannot be separated using other approaches. Collectively, these studies have established QqQ as a powerful and cost-effective platform for molecular imaging of biological systems with unprecedented selectivity.</p><blockquote><p><strong>Presenter:</strong> Sara Amer (University Level Teaching Assistant, Purdue University)</p></blockquote>

Agilent Technologies: Agilent at ASMS 2024 (on demand webinars)

Mass Spectrometry Imaging of Lipid Isobars and Isomers with Unit Mass Resolution

QqQ as a powerful and cost-effective platform for molecular imaging of biological systems with unprecedented selectivity.