Per- and polyfluoroalkyl substances (PFAS) have become a major global concern due to their persistence, bioaccumulation, and potential health impacts. With thousands of known PFAS compounds, and new variants continuing to emerge, identifying and quantifying them across complex environmental and biological samples is an ongoing analytical challenge.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg" alt="Thermo Fisher Scientific: From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg 245w," sizes="100vw" width="1024" height="574"><h3>Why PFAS analysis matters</h3>PFAS are used in everything from firefighting foams to non-stick coatings, yet their chemical stability means they persist in the environment and accumulate in living organisms. Regulators and scientists around the world are racing to better understand their distribution, fate, and toxicity.But with tens of thousands of PFAS, many without available reference standards, traditional targeted analysis alone isn’t enough. Analysts increasingly rely on non-targeted analysis (NTA) workflows powered by high-resolution accurate-mass (HRAM) mass spectrometry and advanced software to uncover the full picture.<h3>Addressing the challenges of PFAS identification</h3>Successful non-targeted PFAS analysis requires overcoming several analytical hurdles:<ol><li>Acquiring high-quality MS¹ and MS² data with ultra-high mass resolution and accuracy.</li><li>Detecting low-abundance PFAS hidden within thousands of background compounds.</li><li>Leveraging comprehensive spectral libraries and databases for confident identification.</li><li>Applying data filtering and scoring tools to assign confidence levels to annotations.</li><li>Using visualization and statistical tools to interpret and compare complex datasets.</li></ol><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Thermo Scientific Compound Discoverer 3.5 software </a>brings together all the components needed for robust PFAS identification—integrating <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=22&amp;manufacturerItems=3">HRAM Orbitrap MS</a> data with an expanded suite of PFAS-specific libraries and databases.In a single workflow, users can:<ul><li>Detect and annotate compounds automatically from LC-MS data.</li><li>Determine possible elemental formulas using isotope pattern and accurate mass information.</li><li>Compare MS² spectra against <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1764">mzCloud</a>, NIST 2023, and in silico PFAS libraries.</li><li>Cross-reference annotations with the EPA PFAS Structure List and the NIST Suspect List of PFAS.</li><li>Use PFAS-specific fragment and neutral loss databases to strengthen identification confidence.</li></ul>With these resources combined, the software enables confident annotation of more than 40,000 PFAS compounds!<h3>New tools for confident, streamlined PFAS identification</h3>Compound Discoverer software introduces several new features designed to simplify and accelerate PFAS NTA workflows:<ul><li>Apply PFAS Scoring Node: Automatically calculates PFAS-specific parameters and a new PFAS Score—no R scripting required.</li><li>PFAS Plot: A visual tool that uses mass-defect metrics to highlight probable PFAS in complex datasets, following methods from Kaufman et al. (2022).</li><li>Expanded suite of libraries and databases: Combines mzCloud, NIST, in silico, PFAS fragment databases, and a new neutral loss database for greater confidence.</li><li>Advanced data visualization: Tools like Kendrick Mass Defect and Molecular Network plots reveal homologous PFAS series and related compound families.</li><li>Built-in statistical analysis tools: Compare PFAS composition between samples using Principal Component Analysis, Differential analysis and Hierarchical Cluster Analysis.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg" alt="Thermo Fisher Scientific From Complexity to Clarity Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg 245w," sizes="100vw" width="800" height="395"><h3>From complexity to clarity</h3>By uniting Orbitrap HRAM data with the most comprehensive PFAS libraries available, Compound Discoverer 3.5 software streamlines non-targeted PFAS analysis—helping laboratories move from data complexity to confident chemical clarity.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5612">Watch the on-demand webinar</a> to see the full workflow in action and learn how Compound Discoverer software can enhance your PFAS analysis.<ul><li>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#PFAS #CompoundDiscoverer #SpectralLibraries</li></ul><blockquote><h5>Zoe Julian</h5>Zoe Julian is a Product Marketing Manager for Chromatography and Mass Spectrometry software at Thermo Fisher Scientific. With over 12 years' experience of working with chromatography and mass spectrometry techniques with a background in GC and GC-MS(/MS) organic environmental analysis, she has helped many laboratories and industries improve their workflows through a unique understanding of their challenges. Transitioning to a focus on Chromeleon CDS she has shown customers how software solutions can improve their productivity and support their day-to-day operations.</blockquote>

From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software

<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

Software

Article

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Application

Academy

Video

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

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LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

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Agilent Technologies

Technology Networks

SelectScience

Waters Corporation

In our third SELECT SERIES MRT in Action deminar join us to see how the exceptional resolving power of the MRT can help you make faster and better-informed decisions on Biopharmaceutical workflows.
During the demo, Dale will show how subtle modifications to primary structure such as differences to the isotope distributions of native and deamidated peptides can be unveiled with the MRT and how the high resolution of the MRT has benefits for protein analysis in that fully-resolved isotope distributions can be obtained, aiding in deconvolution to monoisotopic mass.
The session will conclude with a live Q&amp;A where Dale and other members of the team will be on hand to answer all of your questions. 
Why Should You Attend?
<ul>
<li>Discover how the characteristics of the MRT aid biologics characterization workflows.</li>
<li>Learn how the different acquisition modes work to enhance performance.</li>
<li>Understand how MRT data is processed for various biopharmaceutical workflows.</li>
</ul>
<blockquote>
Presenter: Dale Cooper-Shepherd (Principal Scientist, Advanced Mass Spectrometry, Waters Corporation)
</blockquote>
<blockquote>
Dale is a Senior Scientist in the Discovery MS group at Waters, Wilmslow, UK. He undertook PhD studies at the University of Leeds and conducted postdoctoral research at the University of Oxford, UK. Throughout his training Dale specialized in applying ion mobility MS in the field of structural biology.
</blockquote>
<blockquote>
Dale joined Waters in 2015 and since 2019 has been working as an applications scientist on the SELECT SERIES of instruments.
</blockquote>

Elevated Biologics Characterization

How subtle modifications to primary structure such as differences to the isotope distributions of native and deamidated peptides can be unveiled with the MRT.

Glycoproteins are gaining special recognition in proteomics thanks to their multiple roles in biological processes, be it as biopharmaceuticals or as disease biomarkers in pathological processes.
The bottom-up analysis of glycopeptides therefore offers important advantages for glycoprotein characterization, providing information about the structure and composition of the glycans and about the carrier protein.
In this webinar, our expert speakers will discuss how to optimize glycopeptide analysis using capillary liquid chromatography coupled to tandem mass spectrometry, showcasing how the ZenoTOF 7600 system enhances the analysis of proteins and their post-translational modifications.
Attend this webinar to: 
<ul>
<li>Learn how to design an LC-MS/MS experiment that optimizes acquisition parameters for glycan analysis
</li>
<li>Discover the importance of all steps in the workflow, including peak picking and search parameters during data processing
</li>
<li>Explore how improved parameters boost the annotation of glycopeptides
</li>
</ul>
<blockquote>
Presenter: Dr. Estela Giménez (Associate Professor, University of Barcelona)
</blockquote>
<blockquote>
Presenter: Dr. Maarten Dhaenens (Executive PI ProGenTomics, Ghent University)
</blockquote>

Technology Networks: Improving Peptide Annotation and Glycan Localization Using EAD 

Improving Peptide Annotation and Glycan Localization Using EAD 

How to optimize glycopeptide analysis using capillary liquid chromatography coupled to tandem mass spectrometry.

Improving Peptide Annotation and Glycan Localization

Host-associated microbial communities are unique sources for natural product drug discovery.
By studying these microbes, novel and targeted drug leads can be identified and critical questions answered within the broader field of chemical ecology.
Dr. Balunas’ research connects the dots between microbes, metals and metabolites to discover new medicines for a wide range of human diseases.
In this webinar, Dr. Balunas will showcase her latest research into deriving natural products from the marine invertebrate Botryllus schlosseri.
Attend this webinar to discover:
<ul>
<li>Marine natural product drug discovery
</li>
<li>Advanced informatics tools for metabolomics
</li>
<li>Untargeted metabolomics with timsTOF
</li>
</ul>
<blockquote>
Presenter: Dr. Marcy Balunas (Associate Professor, University of Michigan)
</blockquote>

Technology Networks: Dive Into Microbes, Metals and Metabolites for Drug Discovery

Dive Into Microbes, Metals and Metabolites for Drug Discovery

Latest research into deriving natural products from the marine invertebrate Botryllus schlosseri.

Microbes, Metals and Metabolites for Drug Discovery

Critical to the development and commercialization of a biosimilar is understanding the originator and how its CQAs compare against those of the biosimilar.
Learn more about our solutions that provide high quality peptide mapping, glycan and charge variant data.
<blockquote>
Presenter: Andrew Argo (Strategic Marketing Lead, Waters Corporation)
</blockquote>
<blockquote>
Andrew has held positions across industry with a focus on analytical method development for LC and LCMS applications for products ranging from peptides to gene therapy for almost 10 years. He is currently the strategic marketing lead for the biopharma and new modality portfolios within the chemistry organization, helping to understand these evolving therapeutic areas and customer needs.
</blockquote>

Waters Corporation: Chromatographic techniques for the characterization of CQAs in biosimilars 

Chromatographic techniques for the characterization of CQAs in biosimilars

Solutions that provide high quality peptide mapping, glycan and charge variant data.

Chromatographic techniques for the characterization of CQAs

Many labs are faced with an ever-increasing sample load and changing regulations, plus the need to analyze increasingly complex data, while instrumentation and resources remain at a steady level.
As a consequence, instrument throughput and uptime are critical factors to maintain competitiveness and productivity. You need instruments that have evolved into complete solutions for meeting your current and future challenges.
You can meet your challenges with the new Agilent Revident quadrupole time-of-flight LC/MS system (LC/Q-TOF) and Agilent 6495 triple quadrupole LC/MS (LC/TQ). Their easy-to-use onboard intelligence, harmonized operation, and complete workflow capabilities let you look beyond instrumentation and expand your lab’s potential. So, you can focus on what inspires you and meet every challenge that comes your way.
What&#39;s in it for you?
<ul>
<li>Discover Next-Gen Instruments: Witness the intelligent Agilent Revident quadrupole time-of-flight LC/MS system (LC/Q-TOF) and the Agilent 6495 triple quadrupole LC/MS (LC/TQ). These are more than instruments – they&#39;re your keys to unmatched data precision and analysis.
</li>
<li>Elevate Your Lab&#39;s Potential: Say goodbye to outdated methods and welcome complete intelligent workflows. Picture a lab where time-consuming tasks are streamlined, complex data analysis becomes seamless, and your team can focus on what truly matters.
</li>
<li>Ignite Your Curiosity: Curious minds create transformative science. Learn from industry experts as they dive deep into the realm of instrument intelligence, AI-based tuning, and instrument telemetry. Gain insights into how you can maximize efficiency and enhance the capabilities of your lab.
</li>
</ul>
Who Should Attend? 
<ul>
<li>Lab Managers: Lead your lab into a new era of efficiency and productivity.
</li>
<li>Principle Investigators: Fuel your research with cutting-edge technology and insights.
</li>
<li>Senior Scientists: Stay ahead of the curve by harnessing the power of intelligent instrumentation.
</li>
</ul>

Agilent Technologies: LCMS Intelligence Unveiled - Shaping Futuristic Labs

LCMS Intelligence Unveiled - Shaping Futuristic Labs

Agilent Revident quadrupole time-of-flight LC/MS system (LC/Q-TOF) and Agilent 6495 triple quadrupole LC/MS (LC/TQ).

Monitoring the disinfection byproducts (DBPs) provides essential data to optimize drinking water treatment processes and is critical for compliance with drinking water regulations.
In this webinar, hear Mark Collins, the research and development manager at Northern Ireland Water, present his water catchment profiling study using high-resolution accurate mass spectrometer (HRAM) to screen for unknown disinfection byproducts (DBPs).
Mark&#39;s study initially started with monitoring known contaminants (Acid herbicides). However, following a succession of weather incidents in the catchment area under investigation, this study evolved into investigating the formation of potentially harmful DBPs resulting from the interaction of dissolved organic carbon and water treatment. Mark&#39;s talk is a short look at the present with a solid view for the future.
Learning points:
<ul>
<li>Workflows for unknown contaminant screening
</li>
<li>Generate more data from one injection
</li>
<li>Using HRAM to exceed water regulation requirements
</li>
</ul>
Who should attend:
<ul>
<li>Environmental water testing laboratories
</li>
<li>Laboratory scientists and technicians using LC-MS
</li>
<li>Water and environmental analytical chemistry laboratory managers
</li>
</ul>
<blockquote>
Presenter: Mark Collins (R&amp;D Manager, Northern Ireland Water)
</blockquote>
<blockquote>
Mark Collins has worked in the water industry for over 15 years. He is currently the R&amp;D Manager for Northern Ireland Water where he is focusing on new emerging areas of concern and innovative technologies to support our analytical service provision at NI Water. Mark’s interests are primarily in trace analysis of both organic and inorganic pollutants as well as the study of the creation disinfection by-products, their proliferation and effects on the wider water networks.
</blockquote>

Thermo Scientific: Non-targeted screening to assess drinking water treatment

Non-targeted screening to assess drinking water treatment

Water catchment profiling study using high-resolution accurate mass spectrometer (HRAM) to screen for unknown disinfection byproducts (DBPs).

Join us for this interactive panel discussion where laboratory professionals from across multiple markets and industries share success stories of how they navigated the journey to lab digitalization, from strategy and planning to implementation and optimization.
They will share lessons learned and best practices to best harness the power of digital technologies to improve efficiency, quality, and innovation in the lab.
Plus, you&#39;ll have the opportunity to engage with top managers from Agilent&#39;s lab digitalization team, who will share their expertise and insights on the latest trends, technologies, and best practices. They will provide an overview of Agilent&#39;s digital lab solutions and answer your questions about how to get the most out of your lab&#39;s transformation.
Key learning objectives
<ul>
<li>The benefits and challenges of lab digitalization, and how to overcome common obstacles
</li>
<li>Strategies for selecting and integrating the right digital solutions for your lab&#39;s unique needs
</li>
<li>Best practices for implementing and utilizing your digital lab environment
</li>
</ul>
Who should attend?
<ul>
<li>Laboratory Managers, laboratory directors, IT Managers, business IT, scientists, operations manager</li>
</ul>
<blockquote>
Presenter: Dr. Eliot Randle (SVP Digital Transformation, Digital Lab Consulting)
</blockquote>
<blockquote>
Dr. Eliot Randle is Senior Vice President of Digital Transformation Digital Lab Consulting (DLC). DLC is part of the ProPharma Group and provides independent strategic and business consulting to help organisations to transform their business through better use of data and technology.
</blockquote>
<blockquote>
Presenter: Eric Kemp (IT Manager, Modern Canna)
</blockquote>
<blockquote>
Eric Kemp is a seasoned professional with 15+ years of experience in IT and Analytical Chemistry. He has held diverse roles such as Lab Tech, Analyst, Systems Administrator, Entrepreneur, QA Manager, and IT Manager since 2008. In 2012, Eric founded an IT consulting business specializing in Laboratory Information Management Systems (LIMS) re-implementation for Florida markets. Joining Modern Canna in 2018, Eric has contributed expertise to drive innovation and efficiency in both fields. With a unique perspective at the intersection of IT and Analytical Chemistry, Eric brings valuable insights to the cannabis testing industry.
</blockquote>
<blockquote>
Presenter: Tony McKeown (LIMS Administrator, doTERRA Global)
</blockquote>
<blockquote>
Tony McKeown, from Cobh, County Cork, Ireland, is a highly accomplished individual with a strong background in IT. He graduated from Munster Technological University with a degree in IT and then embarked on a successful 12-year self-employment journey. In September 2021, Tony joined doTERRA Global, based in Blarney, Co Cork, Ireland, where he played a pivotal role in the organization&#39;s digital transformation initiative.
</blockquote>
<blockquote>
Tony&#39;s primary responsibility at doTERRA Global was to identify and implement a Laboratory Information Management System (LIMS) for its onsite laboratories. Leveraging his expertise and knowledge, he successfully sourced and introduced Agilent SLIMS in May 2023, marking a significant milestone in the company&#39;s digitalization journey.
</blockquote>
<blockquote>
Beyond his profession, Tony is an avid sports fan, and has a keen interest in politics and current affairs.
</blockquote>
<blockquote>
Presenter: Jean-Guillaume Baumann (Senior Product Marketing Manager, Digital Lab, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Jean-Guillaume joined Agilent Technologies in 2018 through the acquisition of Genohm as a Business Development Manager. Holding a Master’ degree in Mathematics, he graduated in 2015 from the University of Neuchâtel with a Master’s degree with honors in Mathematics with a specialization in computer science. Passionate about the business side also, he studies for an EMBA at University of Lausanne.
</blockquote>
<blockquote>
Presenter: Dr. Thomas Schmidt (Marketing Director, Digital Solutions, Software &amp; Informatics Division)
</blockquote>
<blockquote>
Thomas Schmidt is Marketing Director for Digital Solutions in the Software &amp; Informatics Division at Agilent Technologies. Originally a chemist by training, he conceptualized and managed multiple Laboratory Informatics solutions during his career covering all aspects of the laboratory operation, including workflow management, data management, sample management, instrument control, and laboratory execution systems. Thomas is a passionate ambassador for the Digital Laboratory.
</blockquote>
<blockquote>
Presenter: Dora Wells (Editorial Team, SelectScience)
</blockquote>
<blockquote>
Dora studied biomedical science at the University of Manchester, UK, and as a member of the editorial team, plays an integral role in helping to shape the content on SelectScience.
</blockquote>

LabRulez/AI: From Paper to Pixels: Success stories of laboratories embracing digitalization

From Paper to Pixels: Success stories of laboratories embracing digitalization

Success stories of how they navigated the journey to lab digitalization, from strategy and planning to implementation and optimization.

Success stories of laboratories embracing digitalization

Multi-residue pesticide analysis presents plenty of challenges on its own, but the expanded matrix list and harmonization of a global company takes it to the next level. We will discuss some of the issues we have faced and the paths taken to address them — with mostly favorable outcomes and a few hard lessons learned.
By attending this webinar you will learn about:
<ul>
<li>NQAC Dublin’s approach to screen over 450 pesticides</li>
<li>Comparison of residues commonly observed versus FDA reporting</li>
<li>Options for problematic matrices</li>
<li>Reference material “Watch outs”</li>
<li>Method portfolio expansion to accommodate customer needs</li>
<li>Changing regulations - local versus global needs</li>
</ul>
<blockquote>
Presenter: Andrew Savage (Expert Chemist, Nestle Quality Assurance Center)
</blockquote>
<blockquote>
Andrew Savage is an Expert Chemist at Nestle Quality Assurance Center in Dublin, Ohio, where he leads projects for new analyses and technology scouting. His focus has been chemical contaminants in food and he was previously the Supervisor of the Contaminants Laboratory, where he managed the performance of 40 trace-level analytical methods. He has 25 years of experience in analytical laboratories within academia, contract research, and in the food industry, working primarily in mass spectrometry. He holds a Bachelor of Science from Mount St. Joseph University in Cincinnati Ohio, PCQI certification, and enjoys sailing in his free time.
</blockquote>
<blockquote>
Presenter: Angela Simon (Pesticides Chemist, Nestle Quality Assurance Center)
</blockquote>
<blockquote>
Angela Simon is a Chemist at Nestle Quality Assurance Center in Dublin, Ohio where she started in the vitamins department and now works in the contaminants department. She works on most, if not all of the pesticide methods at NQAC along with many other methods. She has approximately 18 years of experience in analytical laboratories within environmental, pharmaceutical and the food industry, primarily working with mass spectrometry. She holds a Bachelor of Science degree from West Liberty University in West Liberty, West Virginia and enjoys playing guitar, playing/watching sports, reading, and hiking with her three dogs in her spare time.
</blockquote>

SCIEX: Challenges and solutions while performing pesticide analysis for the world’s largest food and beverage company

Challenges and solutions while performing pesticide analysis for the world’s largest food and beverage company

We will discuss some of the issues we have faced and the paths taken to address them — with mostly favorable outcomes and a few hard lessons learned.

Pesticide analysis for the food and beverage company

<h4>Accelerate time to outcome and achieve efficiency in your laboratory</h4>
Contaminations can enter the food-chain at various stages. This has led to more rigorous regulations for the testing of contaminants in exported, imported, and domestic food and beverages.
In this webinar series, we will discuss how our latest solutions meet industry demands, such as high throughput, enhanced sensitivity requirements, increased accuracy, and compliance.
<h4>Episode 1: Sample preparation strategies and greener alternatives for multi-mycotoxins determination via LC-MS/MS</h4>
The 1st episode is dedicated to the multi-mycotoxins analysis. Here, the focus will be on the modern workflow, with particular focus on combining as many mycotoxins together to increase the efficiency of your laboratory.
During this session we will discuss:
<ul>
<li>Sample extraction options for multi-mycotoxins analysis
</li>
<li>Why efficient sample clean-up is needed?
</li>
<li>How can you minimize contamination and carryover?
</li>
</ul>
At the end of the episode, we will also ask the expert Laura Carbonell Rozas to share insights about her work in natural toxins testing.
Join us for the food safety webinar series, we are really looking forward to answering your questions in our live Q&amp;A session and discussing this topic further with you.
<blockquote>
Presenter: Nicola Dreolin (Senior Scientist and Product Manager, Waters Corporation)
</blockquote>
<blockquote>
Nicola Dreolin is a Senior Scientist and Product Manager at Waters. Nicola is specialised in method development for a variety of applications in food, environmental and life sciences. During the past five years, he was involved in different research projects regarding metabolomics, ion mobility and direct-mass spectrometry. Before joining Waters, Nicola worked as an Analytical Scientist at Elements, where he developed targeted and screening methods for the analysis of extractables and leachables, pharmaceuticals, tobacco and agrochemicals.
</blockquote>
<blockquote>
Presenter: Laura Carbonell Rozas (Postdoctoral Researcher, University of Almeria (Spain))
</blockquote>
<blockquote>
Laura Carbonell Rozas is a Postdoctoral researcher at the University of Almeria (Spain), within the research group “Analytical Chemistry of Contaminants”. Over the past seven years, Laura has been working on developing novel strategies for the determination of a wide range of food and environmental contaminants, including mycotoxins and alkaloids. In her research, Laura also pioneered the investigation of “greener” alternatives to conventional sample preparation methods. She obtained her PhD in Analytical Chemistry at the University of Granada (Spain) and she has worked with multiple international organisations, including LABERCA (France), CONICET (Argentina), and the University of Parma (Italy).
</blockquote>
<blockquote>
Presenter: Janitha De-Alwis (JD) (Principal F&amp;E Market Development Manager, Waters Corporation)
</blockquote>
<blockquote>
Janitha De-Alwis (known as JD) is an applications chemist in the European demo lab, based in Wilmslow, UK. His main role focuses on application development work for the food and environmental sector. JD takes great pride in speaking to, and advising, many food and environmental customers. His position requires him to be reactive and proactive to the changes in the food industry, such as changing regulations, topical issues and food safety risks.
</blockquote>

Waters Corporation: Sample preparation strategies and greener alternatives for multi-mycotoxins determination via LC-MS/MS

Sample preparation strategies and greener alternatives for multi-mycotoxins determination via LC-MS/MS

How our latest solutions meet industry demands, such as high throughput, enhanced sensitivity requirements, increased accuracy, and compliance.

Sample preparation strategies and greener alternatives

Join us for our 3-Part series to Meet our Experts and discuss topics including:
<ul>
<li>History of HPLC column technology over the last 50 years and how advancements have helped address current separation challenges.
</li>
<li>Fundamentals of small molecule separations to enable you to develop methods more efficiently. 
</li>
<li>Solvent and column choices with UPLC to develop more environmentally friendly chromatography methods
</li>
</ul>
<h4>PART 1: In Pursuit of the Ideal HPLC Column: Improving Reproducibility, Stability, Efficiency, and Inertness</h4>
The ideal HPLC column is highly reproducible, stable when exposed to a wide range of mobile phases and temperatures, and able to produce narrow and symmetrical peaks for all analytes of interest. Since the development of the first commercially available HPLC columns 50 years ago, the technologies used to synthesize stationary phases, to fabricate column hardware and to pack columns have steadily improved to allow us to come closer to reaching these ideals.
This presentation will highlight several of the key improvements made over the last 30 years, including high purity stationary phases, hybrid organic/inorganic particles, UHPLC columns and hybrid surface technology hardware. The importance of these advancements for addressing some current separation challenges will be demonstrated.
<blockquote>
Presenter: Tom Walter (Corporate Fellow, Consumables and Lab Automation, Waters Corporation)
</blockquote>
<blockquote>
Thomas Walter received a Ph. D. in Chemistry from the University of Illinois at Urbana-Champaign, working on multinuclear solid-state NMR spectroscopy of heterogeneous catalysts. Joining the Chemistry R&amp;D group of Waters in 1987, he held positions of increasing responsibility, including serving as the Director of Chemistry R&amp;D from 2000 – 2015. He is a coinventor of hybrid organic/inorganic particle technology with 15 issued US patents. He has authored 43 publications on chromatographic materials, columns, and NMR spectroscopy. He is currently a Corporate Fellow in the Consumables and Lab Automation Division. He is a member of the Jim Waters Society and the 2023 recipient of the Uwe Neue award.
</blockquote>

Waters Corporation: In Pursuit of the Ideal HPLC Column: Improving Reproducibility, Stability, Efficiency, and Inertness

In Pursuit of the Ideal HPLC Column: Improving Reproducibility, Stability, Efficiency, and Inertness

Key improvements made over the last 30 years, including high purity stationary phases, hybrid organic/inorganic particles, UHPLC columns and hybrid surface