Per- and polyfluoroalkyl substances (PFAS) have become a major global concern due to their persistence, bioaccumulation, and potential health impacts. With thousands of known PFAS compounds, and new variants continuing to emerge, identifying and quantifying them across complex environmental and biological samples is an ongoing analytical challenge.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg" alt="Thermo Fisher Scientific: From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_2_8e83167891.jpg 245w," sizes="100vw" width="1024" height="574"><h3>Why PFAS analysis matters</h3>PFAS are used in everything from firefighting foams to non-stick coatings, yet their chemical stability means they persist in the environment and accumulate in living organisms. Regulators and scientists around the world are racing to better understand their distribution, fate, and toxicity.But with tens of thousands of PFAS, many without available reference standards, traditional targeted analysis alone isn’t enough. Analysts increasingly rely on non-targeted analysis (NTA) workflows powered by high-resolution accurate-mass (HRAM) mass spectrometry and advanced software to uncover the full picture.<h3>Addressing the challenges of PFAS identification</h3>Successful non-targeted PFAS analysis requires overcoming several analytical hurdles:<ol><li>Acquiring high-quality MS¹ and MS² data with ultra-high mass resolution and accuracy.</li><li>Detecting low-abundance PFAS hidden within thousands of background compounds.</li><li>Leveraging comprehensive spectral libraries and databases for confident identification.</li><li>Applying data filtering and scoring tools to assign confidence levels to annotations.</li><li>Using visualization and statistical tools to interpret and compare complex datasets.</li></ol><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Thermo Scientific Compound Discoverer 3.5 software </a>brings together all the components needed for robust PFAS identification—integrating <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=22&amp;manufacturerItems=3">HRAM Orbitrap MS</a> data with an expanded suite of PFAS-specific libraries and databases.In a single workflow, users can:<ul><li>Detect and annotate compounds automatically from LC-MS data.</li><li>Determine possible elemental formulas using isotope pattern and accurate mass information.</li><li>Compare MS² spectra against <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1764">mzCloud</a>, NIST 2023, and in silico PFAS libraries.</li><li>Cross-reference annotations with the EPA PFAS Structure List and the NIST Suspect List of PFAS.</li><li>Use PFAS-specific fragment and neutral loss databases to strengthen identification confidence.</li></ul>With these resources combined, the software enables confident annotation of more than 40,000 PFAS compounds!<h3>New tools for confident, streamlined PFAS identification</h3>Compound Discoverer software introduces several new features designed to simplify and accelerate PFAS NTA workflows:<ul><li>Apply PFAS Scoring Node: Automatically calculates PFAS-specific parameters and a new PFAS Score—no R scripting required.</li><li>PFAS Plot: A visual tool that uses mass-defect metrics to highlight probable PFAS in complex datasets, following methods from Kaufman et al. (2022).</li><li>Expanded suite of libraries and databases: Combines mzCloud, NIST, in silico, PFAS fragment databases, and a new neutral loss database for greater confidence.</li><li>Advanced data visualization: Tools like Kendrick Mass Defect and Molecular Network plots reveal homologous PFAS series and related compound families.</li><li>Built-in statistical analysis tools: Compare PFAS composition between samples using Principal Component Analysis, Differential analysis and Hierarchical Cluster Analysis.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg" alt="Thermo Fisher Scientific From Complexity to Clarity Smarter PFAS Identification Workflows in Compound Discoverer Software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_From_Complexity_to_Clarity_Smarter_PFAS_Identification_Workflows_in_Compound_Discoverer_Software_ed32c7f7c1.jpg 245w," sizes="100vw" width="800" height="395"><h3>From complexity to clarity</h3>By uniting Orbitrap HRAM data with the most comprehensive PFAS libraries available, Compound Discoverer 3.5 software streamlines non-targeted PFAS analysis—helping laboratories move from data complexity to confident chemical clarity.<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5612">Watch the on-demand webinar</a> to see the full workflow in action and learn how Compound Discoverer software can enhance your PFAS analysis.<ul><li>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#PFAS #CompoundDiscoverer #SpectralLibraries</li></ul><blockquote><h5>Zoe Julian</h5>Zoe Julian is a Product Marketing Manager for Chromatography and Mass Spectrometry software at Thermo Fisher Scientific. With over 12 years' experience of working with chromatography and mass spectrometry techniques with a background in GC and GC-MS(/MS) organic environmental analysis, she has helped many laboratories and industries improve their workflows through a unique understanding of their challenges. Transitioning to a focus on Chromeleon CDS she has shown customers how software solutions can improve their productivity and support their day-to-day operations.</blockquote>

From Complexity to Clarity: Smarter PFAS Identification Workflows in Compound Discoverer Software

<h3>Two Dimensions, One Solution: Advancing Oligonucleotide Analysis with 2D-LC</h3><h4>Why Consider 2D-LC for Oligonucleotide Analysis?</h4>In oligonucleotide analytics, achieving reliable and precise results is not optional—it is fundamental. These biomolecules are inherently complex, often containing truncated sequences, structural variants, and a wide range of impurities. Such complexity frequently exceeds the separation capabilities of conventional one-dimensional liquid chromatography (1D-LC). As a result, critical components may coelute or remain unresolved, limiting analytical confidence.Two-dimensional liquid chromatography (2D-LC) offers a powerful alternative by significantly enhancing separation performance. By combining two complementary separation mechanisms, 2D-LC improves resolution, sensitivity, and overall analytical reliability. This enables a much deeper understanding of oligonucleotide samples and their compositional intricacies.<blockquote><h4>Previous parts from the Oligos Made Easy series</h4><ul><li>Part 1: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6808">Overview</a></li><li>Part 2: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6846">AEX</a></li><li>Part 3: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6880">IP-RP</a></li><li>Part 4: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6912">HILIC</a></li><li>Part 5: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6944">SEC</a></li><li>Part 6: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6982">Column Navigator</a></li><li>Part 7: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7012">Large Scale AEX</a></li><li>Part 8: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7045">Sepapure oliGO</a></li></ul></blockquote><h4>Moving Beyond the Limits of 1D-LC</h4>For many years, 1D-LC has served as a standard tool for biopharmaceutical characterization. However, its inherent limitations become apparent when dealing with highly complex mixtures. The selectivity of a single chromatographic dimension is restricted, and the lack of orthogonality in retention mechanisms often results in insufficient separation. As sample complexity increases, the resolving power of 1D-LC becomes inadequate.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg" alt="KNAUER: Figure 1 - 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_2_D_LC_Principle_Graphic_by_KNAUER_43e04d32a2.jpg 245w," sizes="100vw" width="1291" height="391">Two-dimensional LC addresses these limitations by coupling two chromatographic separations, which can be either similar or fundamentally different. Each dimension targets different physicochemical properties of analytes, enabling the resolution of compounds that would otherwise overlap. In essence, 2D-LC introduces an additional level of separation capacity, allowing analysts to disentangle coeluting species that remain unresolved in a single dimension.An important practical consideration is that the overall analysis time is governed by the second dimension. Because fractions from the first dimension must be processed sequentially, the second dimension must operate rapidly and efficiently. High-speed separations in the second dimension are therefore essential to maintain system performance and data quality.<h4>Architecture of a 2D-LC System</h4>At its core, a 2D-LC system relies on precise coordination between its components. The setup typically consists of two chromatographic columns, two pumping systems, and a switching valve that enables fraction transfer between dimensions. The sample is introduced through an autosampler and first separated on the primary column. Fractions of interest are then transferred into a loop via a switching valve and subsequently introduced into the second column for further separation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg" alt="KNAUER: Figure 2 - 2D LC System Configuration Example (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_2_D_LC_System_Configuration_Example_Graphic_by_KNAUER_f5747adf3a.jpg 245w," sizes="100vw" width="859" height="517">The valve plays a crucial role in this process, acting as the interface between both chromatographic dimensions. Its function is to alternately collect fractions from the first dimension and inject them into the second dimension under high-pressure conditions. Various valve configurations can be employed, including ten-port or eight-port valves with dual-loop arrangements, or synchronized six-port valves. Regardless of configuration, the underlying principle remains the same: while one loop is being filled, the other is being flushed into the second dimension.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg" alt="KNAUER: Figure 3 - Schematic figure of ten-port (left) and eight-port valve (right) 2-position setup for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Schematic_figure_of_ten_port_left_and_eight_port_valve_right_2_position_setup_for_2_D_LC_Graphic_by_KNAUER_9209661ea8.jpg 245w," sizes="100vw" width="1287" height="406">This alternating process requires precise timing. The second-dimension separation must be completed before the next fraction is ready for transfer. Consequently, synchronization between flow rates, loop volumes, and switching intervals is essential for maintaining chromatographic integrity.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg" alt="KNAUER: Figure 4 - Schematic figure KNAUER eight-port 2 position valve for 2D-LC (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_Schematic_figure_KNAUER_eight_port_2_position_valve_for_2_D_LC_Graphic_by_KNAUER_f9a5f06f5f.jpg 245w," sizes="100vw" width="756" height="391"><h4>Applying 2D-LC to Oligonucleotide Analysis</h4>In oligonucleotide workflows, 2D-LC combines two complementary separation strategies to address sample complexity. The first dimension is typically used for bulk separation based on properties such as size, charge, or polarity. Techniques such as size-exclusion chromatography (SEC), anion-exchange chromatography (AEX), ion-pair reversed-phase chromatography (IP-RP), hydrophilic interaction chromatography (HILIC), or mixed-mode chromatography (MMC) are commonly applied. This step reduces sample complexity and can also serve as an online desalting stage, removing salts and buffer components that could interfere with downstream detection, particularly when mass spectrometry is used.Following this initial separation, selected fractions are transferred to the second dimension. This transfer can be performed either selectively or comprehensively, depending on the analytical goal. The second dimension is typically optimized for high-resolution separation, allowing detailed characterization of closely related species such as truncated oligonucleotides, sequence variants, or chemically modified impurities. When coupled with mass spectrometry, this setup enables accurate identification and sequencing.A key advantage of 2D-LC lies in the flexibility of combining different stationary phases and mobile phase conditions.&nbsp;Numerous separation combinations are possible, including:<ul><li>SEC×AEX</li><li>HILIC×IP-RP</li><li>IEC×IP-RP</li><li>IP-RP×IP-RP</li><li>AEX×HIC</li><li>MMC×SEC,</li><li>and even chiral×RP systems.&nbsp;</li></ul>This adaptability allows tailored workflows for specific analytical challenges.<h4>Heart-Cutting vs. Comprehensive 2D-LC</h4>When implementing 2D-LC, two primary transfer strategies are commonly used: heart-cutting and comprehensive transfer. Each approach offers distinct advantages depending on the analytical objective.Heart-cutting 2D-LC focuses on selected regions of interest from the first dimension. Only specific peaks or time windows are transferred to the second dimension for further analysis. This approach allows extended separation time and higher resolution in the second dimension, making it particularly suitable for targeted investigations of known critical compounds. However, because only selected fractions are analyzed, unexpected impurities outside the selected region may remain undetected.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg" alt="KNAUER: Figure 5 - 2D-LC Single Heart-Cut Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_2_D_LC_Single_Heart_Cut_Principle_Graphic_by_KNAUER_b1b400784b.jpg 245w," sizes="100vw" width="1276" height="319">In contrast, comprehensive 2D-LC transfers the entire eluate from the first dimension into the second dimension in a continuous manner. This approach provides a complete and detailed representation of the sample, making it ideal for research and development, impurity profiling, and regulatory applications. While more demanding in terms of speed and system performance, comprehensive 2D-LC ensures full sample coverage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg" alt="KNAUER: Figure 6 - Full Comprehensive 2D-LC Principle (Graphic by KNAUER)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_Full_Comprehensive_2_D_LC_Principle_Graphic_by_KNAUER_e13da63ef1.jpg 245w," sizes="100vw" width="1327" height="292">In practice, hybrid approaches may also be employed, combining targeted heart-cutting with broader comprehensive analysis to balance resolution and coverage.<h4>Practical Considerations for Method Development</h4>Successful implementation of 2D-LC for oligonucleotide analysis requires careful method design.&nbsp;<h5>1. Column selection&nbsp;</h5>It is a critical starting point, as the combination of stationary phases must provide sufficient orthogonality while maintaining compatibility between mobile phases. Typically, the first dimension is optimized for general separation of oligonucleotide size or class, while the second dimension focuses on sequence-specific resolution and compatibility with mass spectrometry.<h5>2. Mobile phase selection&nbsp;</h5>Differences in pH and organic modifiers can significantly influence separation behavior, but incompatibility between dimensions can compromise performance. Therefore, mobile phases must be designed as a coordinated system. The use of volatile buffers, such as ammonium acetate, is often preferred for MS-based detection.<h5>3. Instrumentation&nbsp;</h5>The switching valve must operate reliably under high pressure, with precise timing to ensure accurate fraction transfer. Loop volumes must be carefully selected to avoid overfilling, as excessive loading can lead to sample loss and distortion due to flow profile effects. As a general guideline, loops should not be filled beyond approximately 70% of their capacity.<h5>4. Method development&nbsp;</h5>Typically begins with optimization of the second dimension, as it dictates overall system timing. Fast separations using short columns and steep gradients are often required to maintain sufficient sampling frequency. Once the second dimension is established, the first dimension can be adjusted to achieve optimal resolution and compatibility.<h4>Final Remarks</h4>Two-dimensional liquid chromatography represents a powerful advancement in oligonucleotide analysis, enabling the resolution of complex mixtures that exceed the capabilities of conventional 1D approaches. By combining orthogonal separation mechanisms, 2D-LC reveals structural details and impurity profiles that would otherwise remain hidden.However, achieving optimal performance requires careful balancing of multiple factors, including column chemistry, mobile phase compatibility, system timing, and data acquisition. When properly implemented, 2D-LC provides a robust and highly informative platform for modern oligonucleotide characterization.<blockquote><ul><li>If you're planning to set up or optimize your oligonucleotide workflow, feel free to contact us at <a href="sales@knauer.net">sales@knauer.net</a>. Stay tuned for more exciting insights into the Oligonucleotide world in our “Oligos Made Easy” series.</li><li>For more in-depth discussion or questions, reach out to the author at <a href="kindler@knauer.net">kindler@knauer.net</a></li></ul></blockquote>

Oligos Made Easy - Part 9: 2D-LC

News from LabRulezLCMS Library - Week 14, 2026

Software

Article

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Application

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Video

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

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LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

Pittcon

SelectScience

Shimadzu Corporation

Evosep Biosystems

Waters Corporation

Supramolecular structures formed from bioactive and responsive polymers can be used as smart carrier systems for enzymes or drugs. Given the inherent heterogeneity of these complex nanostructures, their characterization is a significant challenge.
In this exclusive webinar, join experts Dr. Susanne Boye and Dr. Robert Mildner, to learn more about asymmetric-flow field-flow fractionation (AF4), a versatile, state-of-the-art separation technique for simplifying sample matrices, enabling subsequent microstructural analysis. Coupled AF4 with multiple online detectors (multi-angle light scattering, dynamic light scattering, differential refractometer, UV/Vis and differential viscometer) provides rich data for multidimensional characterization and correlation.
The MD-AF4 analytical platform supports in-depth physical and chemical understanding of natural and synthetic macromolecules, their self-assemblies and bioconjugates. Dr. Boye and Dr. Mildner will discuss MD-AF4 and its applications to polymer-protein hybrids, exosome-polymer-hybrids, stimuli-responsive polymeric vesicles and single chain nanoparticles.
Key learning objectives
<ul>
<li>Learn more about the background and instrumentation for separation and analysis by MD-AF4
</li>
<li>Find out how MD-AF4 determines physical and chemical parameters of natural and synthetic macromolecules
</li>
<li>Discover how MD-AF4 is used to analyze self-assemblies and bioconjugates
</li>
</ul>
Who should attend?
<ul>
<li>Chemists and nanoengineers requiring in-depth characterization of complex polymeric and nanoparticle systems
</li>
<li>Drug-development scientists exploring analytical instrumentation for nano-drug delivery vehicles
</li>
</ul>
<blockquote>
Presenter: Susanne Boye, PhD (Center for Macromolecular Structure Analysis)
</blockquote>
<blockquote>
Dr. Susanne Boye is the leader of the Field-flow Fractionation team of the Polymer Separation Group, the leader of the Thermal Analysis Lab and deputy head of the Center for Macromolecular Structure Analysis of the Leibniz Institute of Polymer Research Dresden (IPF), Germany. She graduated in biochemical engineering and obtained a PhD in the polymer separation field at the Technical University Dresden and IPF. Dr. Boye is particularly interested in the conformation analysis of biomacromolecules and the physico-chemical characterization of polymer-protein conjugates, complex synthetic and natural (bio-) nanostructures and molecular interactions, with more than 60 publications in these fields.
</blockquote>
<blockquote>
Presenter: Robert Mildner, PhD (Waters | Wyatt Technology)
</blockquote>
<blockquote>
Dr. Robert Mildner is an Application Scientist at Wyatt Technology in Europe, where he is responsible for performing research projects and feasibility studies utilizing Wyatt´s unique instrumentation. His work includes testing of novel applications as well as the implementation of established methods for users of Wyatt instruments. He focuses on field-flow fractionation (FFF) and size-exclusion chromatography (SEC) coupled to multi-angle light scattering (MALS). Robert obtained his PhD from the University of Technology Braunschweig, Germany, in the field of macromolecular chemistry.
</blockquote>
<blockquote>
Presenter. Carrie Haslam (SelectScience)
</blockquote>
<blockquote>
Dr. Carrie Haslam is an Associate Editor at SelectScience, playing a key role in content production and specializing in Materials Science, Alzheimer’s disease and Clinical Diagnostics. Carrie completed a Ph.D. from The University of Plymouth, where she developed graphene-based biosensors for the early diagnosis of Alzheimer’s disease.
</blockquote>

Waters Corporation: Unraveling the complexity: Advanced analysis of polymeric nanostructures

Unraveling the complexity: Advanced analysis of polymeric nanostructures

Learn more about asymmetric-flow field-flow fractionation (AF4).

Advanced analysis of polymeric nanostructures

The identification and quantification of compounds in biological samples are commonly performed using a technique called reversed phase-HPLC- hyphenated to mass spectrometric detection (RP-HPLC-MS).
However, when authentic standards are not available, quantification becomes difficult due to the analyte-specific response of the detector, as the relative signal intensities in electrospray ionization-MS are dependent on ionization efficiency.
The choice of an appropriate standard is further limited by various parameters such as the chemical and physical properties of the analyte or the retention behavior.
Additionally, in lipid research, the analytes often lack chromophoric groups, which means that UV/vis detection is not feasible, or additional derivatization procedures become inevitable.
To overcome these challenges, a complementary quantification strategy called charged aerosol detection (CAD) is employed. CAD offers a consistent and uniform response for nearly all non-volatile substances, whilst ensuring both mass sensitivity and robustness.
In this webinar, quantification strategies without authentic standards based on HPLC-CAD will be showcased for determination of several lipid classes in complex sample matrices. Examples of these lipid classes include biosurfactants like rhamnolipids and liamocins, as well as membrane lipids such as phospholipids.
Learning points:
<ul>
<li>What makes charged aerosol detection so powerful
</li>
<li>What benefits it offers
</li>
<li>What applications are ideal for charged aerosol detection?
</li>
</ul>
Who should attend:
<ul>
<li>Laboratory managers
</li>
<li>Technology leaders
</li>
<li>Principal scientists
</li>
<li>Laboratory analyst
</li>
</ul>
<blockquote>
Presenter: Prof. Dr. Heiko Hayen (Professor, Institute of Inorganic and Analytical Chemistry, University of Münster)
</blockquote>
<blockquote>
Heiko Hayen is professor for Analytical Chemistry at the University of Münster since 2014. He studied Food Chemistry and obtained his PhD in Analytical Chemistry at the University of Twente (Enschede, The Netherlands) in 2003.
</blockquote>
<blockquote>
After a postdoctoral research stay at the Institute for Marine Biosciences (National Research Council of Canada, Halifax, Nova Scotia, Canada), he moved to the Department of Bioanalytics, Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V., Dortmund.
</blockquote>
<blockquote>
From 2010 to 2014 he held a professorship in Food Chemistry at the Faculty of Mathematics and Natural Sciences, University of Wuppertal.
</blockquote>
<blockquote>
His current research interests include the development of hyphenated mass spectrometric methods for the investigation of metabolites and lipids, like phospholipids and glycolipids, using HPLC-MS, SFC-MS and capillary IC-MS.
</blockquote>
<blockquote>
Presenter: Chris Tuczemskyi (Moderator, Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Chris Tuczemskyi is a chromatography specialist at Thermo Fisher Scientific, based in Cambridge, UK. After spending a number of years building up his experience with chromatography instruments in a water testing laboratory, he joined Thermo Fisher Scientific in 2014 as a chromatography service engineer for Unity Lab Services. Here he developed his passion for supporting customers using HPLC and in 2018 moved into the application specialist team. More recently, in 2022 he made the move to Product Marketing to help ensure we meet and exceed our customers expectation. In 2009, Chris graduated from the University of Essex in Biochemistry (Bsc Hons).
</blockquote>

Thermo Scientific: Absolute quantification of lipids in complex mixtures by HPLC without authentic standards

Absolute quantification of lipids in complex mixtures by HPLC without authentic standards

Quantification strategies without authentic standards based on HPLC-CAD will be showcased for determination of several lipid classes in complex sample matrices.

Absolute quantification of lipids

Immunopeptidomics is an emerging field that combines proteomics and immunology to study the complex repertoire of peptides presented by major histocompatibility complex (MHC) molecules on the cell surface.
These MHC-bound peptides, known as immunopeptidomes, play crucial roles in immune recognition and activation, influencing various physiological and pathological processes such as immune surveillance, infectious diseases, autoimmunity, and cancer.
Join our webinar to explore recent advancements, challenges, and applications in immunopeptidomics. Discover how Evosep One accelerates discoveries, and support the understanding of immune responses and diseases.
<blockquote>
Presenter: Anthony Purcell (Professor, Purcell Lab, Monsah University)
</blockquote>
<blockquote>
Presenter: Nicolai Bache (Head of Applications at Evosep)
</blockquote>

Advancing Immunopeptidomics

Recent advancements, challenges, and applications in immunopeptidomics.

<h5>Solving Problems that Matter: Risk Mitigation in the Pharma QC Laboratory</h5>
Expert Insights 3-Part Webinar Series: Risk Assessment, Control Strategies, and Intuitive Innovation to Improve Data Quality, Data Integrity and Lab Productivity. 
Pharma QC labs are facing increasing pressure on the cost of quality while maintaining compliance and assuring patient safety. From the harmonization of USP &lt;621&gt; to Empower™ workflows supporting data integrity to improving method transfer outcomes, you will learn from subject matter experts how to reduce errors and mitigate risk for reproducible data and enhanced productivity. 
Who should attend
<ul>
<li>Pharma QC Lab directors, managers and senior scientists with a drive to reduce errors, improve lab productivity and enhance data quality &amp; integrity.</li>
</ul>
<h3>PART 3 l Creating Efficiency in Your Routine Workflow with Simple Automation Steps in Empower</h3>
<ul>
<li>Wednesday, August 30th, 11:30-12:30 ET</li>
</ul>
According to the PIC/S guidance, complex or open-ended processes that lead to subjective interpretation can result in an increase in data failures. One way to reduce this risk is with a simplified, well-defined process. Empower software enables you with tools to create structure in your chromatographic processes and workflow.
In this presentation we will define a chromatographic workflow with Empower software to support data integrity goals - starting with method development, method validation, and routine usage to data acquisition, data processing, reporting results, and using control charts to track and trend data. This presentation will also introduce new features coming to Empower 3.8.0 such as safeguards to prevent common errors, enhanced column tracking &amp; history and more detailed audit trails.
<blockquote>
Presenter: Neil Lander (Principal Product Manager - Informatics Group, Waters Corporation)
</blockquote>
<blockquote>
Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager in Waters in the Informatics group. Neil provides internal support for Empower CDS software and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters Corporation, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.
</blockquote>

Waters Corporation: Creating Efficiency in Your Routine Workflow with Simple Automation Steps in Empower

Creating Efficiency in Your Routine Workflow with Simple Automation Steps in Empower

Chromatographic workflow with Empower software to support data integrity goals.

Creating Efficiency in Your Routine Workflow

Experience faster, more efficient, and higher quality purification processes with Shimadzu&#39;s Preparative LC and SFC Solutions.
It’s time to streamline your workflow with Shimadzu&#39;s leading-edge Preparative LC and SFC solutions. Our Nexera Prep products offer complete solutions that produce clean and reproducible isolates, with a user-friendly workflow that accommodates all skill levels. Here&#39;s how Shimadzu&#39;s Nexera Prep can help you save time and boost productivity:
<ul>
<li>Nexera Prep Standard – Fully equipped with advanced functions that ensure reliable purification of target components.
</li>
<li>Nexera MDAP – Capable of high purity preparative work with no target components left behind.
</li>
<li>Nexera UFPLC – Equipped with trap technology for concentration, purification, and collection in 100% organic solvent, reducing overall processing time.
</li>
<li>ASAPrep – Automatic scale-up from analytical to preparative scale, saving time and resources needed for optimization.
</li>
</ul>
Join us in the live webinar to learn how Shimadzu&#39;s Nexera Prep can streamline your workflow and produce outstanding results with minimal effort and time. Don&#39;t miss out on this opportunity – save your seat now!
Learning Objectives:
<ul>
<li>Understand how purification workflow can be streamlined for higher efficiency
</li>
<li>Explore Shimadzu’s leading-edge Preparative LC and SFC solutions
</li>
<li>Learn how automation plays a role in eliminating tedious and manual work
</li>
</ul>
<blockquote>
Presenter: Kosuke Nakajima (Innovation Centre Manager, Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Kosuke Nakajima has strong technical knowledge in separation science, particularly LC, LC/MS, and Preparative LC system. Before joining Shimadzu Asia Pacific as an Innovation Centre Manager, he was an application chemist at Shimadzu Corporation Japan, and contributed to developing some novel analytical methods. His recent research has mainly focused on Automated Method Development, Workflow enhancement for Preparative LC, and Supercritical Fluid Chromatography. He has earned his Master&#39;s in Chemistry back in 2016.
</blockquote>
<blockquote>
Presenter: Liw Wan Tung (Assistant Product Manager (LC/Pharma), Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Wan Tung, is the LC Product Manager and Pharmaceutical market in-charge at Shimadzu Asia Pacific Pte Ltd. She is the LC expert with more than 14 years of working experience in analytical science, specializing in method development of various LC and LCMS applications. In 2012, she joined Shimadzu to develop end-to-end workflow solutions for the customers. She has supported a multitude of applications from impurity analysis to drug discovery and biopharmaceutical applications. Prior to joining Shimadzu, Wan Tung started her professional career as a QAQC chemist in Pharmaceutical multinational company. Wan Tung earned her bachelor’s honour&#39;s degree in chemistry back in 2009.
</blockquote>
<blockquote>
Presenter: Navin Devadiga, Ph.D (Assistant Manager (Drug Discovery), Shimadzu Analytical India)
</blockquote>
<blockquote>
Navin Devadiga currently works as an Assistant Manager at Shimadzu Analytical India, where he focuses on method development for the analytical division in the Drug Discovery segment. With over 14 years of experience in the analytical industry, he has gained specialized knowledge in advanced analytical instrumentation, particularly in LC, Preparative LC, LC-MS, and SFC technology. His responsibilities also include providing innovative solutions to drug discovery laboratories and pharmaceutical companies through the use of advanced analytical technologies. Prior to joining Shimadzu, Navin worked as an R&amp;D scientist at a multinational pharmaceutical company. During this time, he actively contributed to the development of analytical methods for identifying process-related impurities, degradation impurities, and genotoxic impurities in drug substances and drug products.
</blockquote>

Shimadzu: Streamlining Your Purification Workflow With Automation

Streamlining Your Purification Workflow With Automation

How Shimadzu's Nexera Prep can streamline your workflow and produce outstanding results with minimal effort and time.

Immunopeptidomics is a field that combines proteomics and immunology to study the complex repertoire of peptides presented by major histocompatibility complex (MHC) molecules on the cell surface.
These MHC-bound peptides, known as immunopeptidomes, play crucial roles in immune recognition and activation, influencing various physiological and pathological processes such as immune surveillance, infectious diseases, autoimmunity, and cancer.
Join our webinar to explore recent advancements, challenges, and applications in immunopeptidomics. Discover how EvosepOne accelerates discoveries, and support the understanding of immune responses and diseases.
<h4>IMMUNOPEPTIDOMICS ON THE EVOSEP ONE – NEW LEVELS OF SAMPLE THROUGHPUT AND PRODUCTIVITY</h4>
Immunopeptidomics is the study of peptide antigens that are bound to and presented on the cell surface by molecules encoded by the Major Histocompatibility, a highly polymorphic region of the genome that encode molecules involved in immune responses. These peptides are derived from both intracellular and extracellular degradation of the parental proteins, generating a highly complex repertoire of peptide ligands, including peptides derived from pathogens, tissue specific and cancer specific antigens when relevant. In humans, human leukocyte antigen (HLA) class I and class II molecules are of particular interest for immunopeptidomic analysis. These molecules are encoded by several loci and constitute thousands different alleles that vary between individuals. Thus, immunopeptidomes are highly individualized and almost impossible to accurately predict. This predicates the empirical measurement of the complexity and diversity of immunopeptidomes using mass spectrometry. Due to the relatively small amounts of material available for analysis the use of nanoflow LC-MS for immunopeptidome analysis is critical. Here I explore the implementation of the high throughput EvoSEP one platform couples to a Bruker timsTOF Pro 2 for robust analysis of immunopeptidomes across a variety of tissues and cell lines. Using the Whisper 20SPD method data quality and depth is maintained whilst sample carryover is negligible allowing more productive use of instrument time; I highlight significant improvements in throughput with over 1500 immunopeptidomes characterised in just 3 months.
<blockquote>
Presenter: Anthony Purcell (Professor, Purcel Lab, Monsah University)
</blockquote>
<blockquote>
Presenter: Nicolai Bache (Head of Applications at Evosep)
</blockquote>
<h4>MAPPS: High throughput immunopeptidomics for the assessment of the immunogenic potential of protein biotherapeutics</h4>
Biologics, mostly monoclonal antibodies and recombinant proteins, are a class of drugs used to treat some particularly difficult conditions. These proteins, when recognized as non-self by the immune system, can elicit unwanted immunogenicity. One of the first steps in this cascade is the presentation of fragments of this protein by MHC complexes on antigen presenting cells, which might ultimately lead to generation of anti-drug antibodies (ADAs). These ADAs can lower the therapeutic efficiency and can lead to severe immunogenic effects. To bring safe biotherapeutics to the market it is critical to thoroughly assess the immunogenic profile of a biotherapeutic. MAPPS (MHC associated peptides proteomics) assays are the method of choice to define all potential immunogenic regions of a protein. ImmuneSpec, an advanced immunopeptidomics platform, offers MAPPS as a service. Here we show how the combination of high-throughput MHC peptide purification, fast LC using EvoSep, and high sensitive MS addresses these needs.
<blockquote>
Presenter: Kurt Boonen (Assistant Professor, Deparment of biomedical sciences, University of Antwerp)
</blockquote>
<blockquote>
Presenter: Nicolai Bache (Head of Applications at Evosep)
</blockquote>

Evosep: ADVANCING IMMUNOPEPTIDOMICS: LEVERAGING EVOSEP ONE FOR HIGH-THROUGHPUT ANALYSIS AND DISCOVERY

ADVANCING IMMUNOPEPTIDOMICS: LEVERAGING EVOSEP ONE FOR HIGH-THROUGHPUT ANALYSIS AND DISCOVERY

Immunopeptidomics: HIGH-THROUGHPUT ANALYSIS AND DISCOVERY

<h3>A New Era of Intuitive Simplicity</h3>
In March 2023 at Pittcon, Waters launched the Alliance iS HPLC System, designed specifically to help QC labs address their biggest challenges around error reduction, workflow efficiency, and compliance.
In this webinar, Debbie Francis, Principal Consulting Product Marketing Manager, QA/QC at Waters, will present some highlights from the product launch at this year’s Pittcon, and demonstrate a new, free tool that will help you to calculate approximately how much time and money the Alliance iS HPLC System could save your lab.
Agenda:
<ul>
<li>Overview of the new product from Fraser McLeod, Vice President of QA/QC at Waters, recorded live at Pittcon 2023
</li>
<li>Fireside chat with Fraser and Dr. Tina S. Morris, Executive Director of the American Association of Pharmaceutical Scientists (AAPS), recorded live at Pittcon 2023
</li>
<li>Demo of the ROI Calculator, a new tool that helps you see just how much time and money the Alliance iS HPLC System can save your lab.
</li>
</ul>
<blockquote>
Presenter: Debbie Francis (Principal Consulting Product Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Debbie has worked for Waters since 2018 and has held multiple product marketing positions for Mass Spectrometry systems and is currently Principal Consulting Product Marketing Manager in QA/QC, focusing on next generation systems, where she has been leading the commercialization of the new era of simplicity in HPLC. Debbie began her career as a scientist in clinical laboratories, before moving into marketing, and prior to joining Waters held various regional and global marketing roles within the life science industry.
</blockquote>

Waters Corporation: Introducing the Alliance iS HPLC System

Introducing the Alliance iS HPLC System

How much time and money the Alliance iS HPLC System could save your lab.

UV-Vis spectrophotometric analysis is vital for Pharma and Biopharma labs, advancing drug development, quality control, and research.
Explore how the Agilent Cary 3500, in synergy with OpenLab Software, conquers challenges, ensures data integrity, and maximizes productivity. Its modular design tailored to your application needs streamlines complex measurements with minimal sample preparation. With an advanced xenon flash lamp offering a 10-year replacement warranty and no warm-up time, rest assured your sensitive samples are safeguarded for reliable and cost-effective analysis.
Learning Objectives:
<ol>
<li>Overcoming Challenges with Long Pathlength Analysis
</li>
<li>Latest Technology for Solid Sample Analysis
</li>
<li>Simultaneous Measurements with Temperature Control using Multicell
</li>
<li>Simplifying Complex Measurements for Protein and Biological Samples
</li>
<li>Enhancing Laboratory Productivity, Cost Saving, and Ease of Use
</li>
<li>Achieving Data Integrity and Compliance with Global Regulations
</li>
</ol>
Who should attend?
<ul>
<li>Pharma &amp; Biopharma professionals
</li>
<li>QA/QC laboratory analysts and managers
</li>
<li>Researchers, academicians and students in Life Sciences
</li>
</ul>
<blockquote>
Presenter: Shubham Sengupta (Product Specialist – Spectroscopy, Agilent Technologies)
</blockquote>
<blockquote>
Shubham Sengupta is a Spectroscopy Product Specialist at Agilent Technologies, with 14 years of diverse experience ranging from CRO to CTL. He holds a postgraduate degree in Chemistry from Indian School of Mines, Dhanbad (IIT-Dhanbad).
</blockquote>

Agilent Technologies: Powering UV-Vis Analysis in Pharma & Biopharma: Agilent Cary 3500

Powering UV-Vis Analysis in Pharma & Biopharma: Agilent Cary 3500

Overcoming Challenges with Long Pathlength Analysis.

Powering UV-Vis Analysis in Pharma & Biopharma

In this session, explore the operation and latest developments of automated SPME for VOCs and SVOCs by headspace, direct immersion, and derivatization techniques.
Furthermore, discover how SPME has key applications for food, water, environmental and forensic samples for use in laboratories for GC-MS and LC-MS and direct MS analysis.
<blockquote>
Presenter: Hans-Joachim Huebschmann (Consultant, VP Sample Preparation, CTC Analytics)
</blockquote>

SelectScience: Solid phase microextraction – How does it work for my samples?

Solid phase microextraction – How does it work for my samples?

Explore the operation and latest developments of automated SPME for VOCs and SVOCs.

Solid phase microextraction–How does it work for my samples?

Were you unable to attend your local pharma day? If so not to worry!
We are delighted to host our popular pharma seminar day online. Following a series of events across Europe we are now bringing the talks to you virtually this September.
<h3>Overview</h3>
The analytical challenges within pharma are constantly changing and evolving. In many laboratories the requirements to deliver fast, accurate and robust results is always present and often the workload is growing too. The webinar will focus on analytical challenges and workflows in pharma analysis to help pharma labs succeed in these challenges.
On the day, you will be able to:
<ul>
<li>Hear about Waters’ latest innovation bringing ultimate simplicity to routine HPLC separations
</li>
<li>Learn about the benefits and tools for risk assessment and mitigation in your QC lab
</li>
<li>Understand the latest in AQbD requirements and how to apply them in your lab
</li>
<li>See the latest trace impurity quantification workflows, including nitrosamines analysis
</li>
</ul>
<h3>Agenda</h3>
<h5>Risk Assessment and Mitigation for Analytical Procedures</h5>
<ul>
<li>Andrea Gheduzzi (Principal Pharma Market Development Manager-EMEA, Waters)</li>
</ul>
<h5>Introducing a New Era on Intuitive Simplicity for Routine HPLC Alliance iS and System monitoring</h5>
<ul>
<li>Arjan Timmerman (Principal Pharma Market Development Manager – EMEA, Waters)</li>
</ul>
<h5>Nitrosamines and Beyond. Technologies for Optimizing Performance and Minimizing Risk in Trace Impurity Quantification</h5>
<ul>
<li>Antonia Wierzbicki (Principal Pharma Market Development Manager- EMEA, Waters)</li>
</ul>
<h5>Live Q&amp;A session</h5>
<blockquote>
Presenter: Andrea Gheduzzi (Southern Europe Pharma Market Dev. Manager, Waters Corporation
</blockquote>
<blockquote>
Andrea has spent more than 30 years in the chromatography field. Across his career he had various roles, service engineer, chromatography lab position, and technical application support manager. He has mainly been dealing with pharma accounts, also supporting LC method development activities using DoE/QbD approaches. In his role as Pharma Business Development Manager at Waters he has further expanded his expertise in AQbD and related applications.
</blockquote>
<blockquote>
Presenter: Arjan Timmerman (Pharma Market Development Manager EMEA, Waters Corporation)
</blockquote>
<blockquote>
Arjan is an experienced business development manager with a demonstrated history of working in regulated pharmaceutical industry. He holds a BSc in analytical chemistry and is skilled in life sciences, method development &amp; validation, laboratory informatics, compliance and data integrity.
</blockquote>
Within Waters Europe, he is a business development manager in core markets focusing on compliance and regulatory challenges (e.g. data integrity for laboratories), impurity analyses and recently on extractables &amp; leachables.
<blockquote>
Presenter: Antonia Wierzbicki (Pharma Market Devlopment Manager EMEA, Waters Corporation)
</blockquote>
<blockquote>
Antonia Wierzbicki is a marketing manager at Waters working in EMEA. Antonia has BSc in Pharmaceutical Science and a MSc in Cancer Pharmacology. Following several years working as an analytical chemist in drug development roles within both academic labs and contract research organizations, Antonia has more recently spent time working with analytical chemists to provide solutions using cutting edge technologies to meet their needs and requirements, with a specific interest in ultra-sensitive quantitation of small molecules. Antonia is based in Cambridge UK and keen to connect with like-minded scientists in the field.
</blockquote>

Waters Corporation: Waters Virtual Pharma Day - Analytical Challenges and Workflows in Pharma Analysis

Waters Virtual Pharma Day - Analytical Challenges and Workflows in Pharma Analysis

We are delighted to host our popular pharma seminar day online. Following a series of events across Europe we are now bringing the talks to you virtually.