<p><strong>Professor Michaela Rumlová, head of a research group of the National Institute of Virology and Bacteriology at UCT Prague (and Vice-Rector for doctoral studies), holder of a licensed patent &nbsp;for antiretroviral testing assays, and recipient of the Czech Science Foundation’s President’s Award for HIV research, has also become the principal investigator of the bilateral INTER-EXCELLENCE II project “</strong><i><strong>Cellular factors regulating the export and packaging of unspliced ​​retroviral RNA</strong></i><strong>”, starting this year. In this interview, she explains how retroviruses such as HIV “hack” the cell in order to export their own genome from the nucleus into the cytoplasm, and then to the site where it is specifically packaged into nascent viral particles, a key step in viral replication. Her team is collaborating with the NIH (National Institutes of Health) and Rockefeller University to elucidate mechanisms that may help reveal new vulnerable points in the retrovirus replication cycle while simultaneously clarifying related cellular processes.</strong></p><h5><strong>Could you describe your project and its main objectives?</strong></h5><p>Retroviruses, such as HIV, are viruses that carry genetic information in the form of RNA; however, after entering a cell, they are able to reverse transcribe it into DNA and integrate it into the host genome. In order to replicate, they must transport their genetic information—in the form of unspliced RNA—from the nucleus into the cytosol. There, it serves both as a template for the production of viral proteins and as the genome of new viral particles. This is highly unusual, as the cell very carefully controls which RNA molecules are allowed to leave the nucleus. Normal protein-coding cellular RNAs, known as messenger RNAs (mRNAs), must undergo splicing—a processing step in which certain segments are removed—before being exported. When this process fails, mRNA that retains these segments is held back in the nucleus as part of quality control and subsequently degraded. Retroviruses have managed to outwit this cellular control machinery, allowing their unspliced RNA to pass into the cytoplasm.</p><p>Our project focuses on identifying the cellular proteins that assist retroviruses in this step and on the mechanism by which protein complexes bound to viral RNA are remodelled during RNA transport. The goal is to understand how the virus uses cellular mechanisms to its advantage and how this pathway is linked to the subsequent fate of the RNA, i.e. to its translation and packaging into new viral particles.</p><h5><strong>Why is this export of unspliced ​​RNA so important for retroviral replication?</strong></h5><p>Because this RNA has a dual role for the virus. On the one hand, it serves as a template for synthesizing viral proteins in the cytoplasm; on the other, it represents the genetic material itself, which must be packaged into new viral particles. The virus must package its RNA genome in its unspliced form, as only this RNA contains essential sequences without which further viral replication would be impossible.</p><p><strong>Simply put: </strong>the virus needs to get a complete copy of its genetic instructions out of the nucleus. Once the viral RNA is in the cytoplasm, it can be used as a template for synthesizing viral proteins; and a portion of these RNA molecules is then selected and packaged into a new particle as a genome. We are interested in how the virus forces the cell to allow viral RNA that is, from the cell’s perspective, “unfinished,” that is unspliced, to leave the nucleus. We are also investigating how the cell and the virus together - albeit unintentionally from the cell’s perspective - determine which RNA molecule will be translated into proteins and which will be packaged as the genome. This is one of the key, yet still not fully understood, steps of retroviral replication.</p><h5><strong>What do you think it was about your project that impressed the Ministry of Education, Youth and Sports’ jury the most?</strong></h5><p>I believe the decisive factor was a combination of several elements. First, the reviewers were primarily interested in the scientific problem itself. We are investigating a mechanism that is essential for retroviruses, but still not sufficiently understood, which may also have broader implications for the regulation of cellular processes. In addition, the quality of the team, our strong collaboration with the<strong> NIH </strong>and <strong>Rockefeller University</strong>, and the use of modern methods that will allow us to track viral RNA in great detail also played a role. It was also important that the project has clear objectives and a realistic plan.</p><h5><strong>How will this research contribute to the development of new treatments for retroviral infections?</strong></h5><p>It is important to say that this is primarily basic research. Rather than developing a new drug directly, we are trying to understand, at the molecular level, the step that the virus cannot do without. Only when we fully understand the mechanism of these cellular processes and identify the essential factors for the export of unspliced ​​RNA and how they function, will we be able to better determine which interactions or mechanisms represent potentially vulnerable points. Similar mechanisms associated with the export of unspliced ​​mRNA also appear in some diseases, such as cancer, so I &nbsp;see the benefit of our research mainly in that it will refine our understanding of these pathological processes in the cell, and retroviruses help us do so. Therefore, our work on retroviruses not only shows us how the virus works, but also how the cell itself works.</p><h5><strong>What responsibilities does your UCT Prague team have? How does the collaboration with your American partners work?</strong></h5><p>At UCT Prague, we are conducting the project’s primary experimental work. Our research involves cell cultures, viral models, and various biochemical techniques ranging from immunochemistry to microscopy that allow us to track viral RNA and identify its cellular partners. In collaboration with colleagues from <strong>NIH, Frederick</strong>, we are developing single-particle tracking methods to observe viral RNA movement in living cells across both space and time. Furthermore, we are working with colleagues from <strong>Rockefeller University to employ iCLIP </strong>methods for the precise mapping of protein-binding sites on RNA. Both collaborations are valuable to us, because they are not just about data sharing, but also about the transfer of expertise and detailed know-how.</p><h5><strong>Who else is collaborating on the project?</strong></h5><p>At UCT Prague, we collaborate closely with the Department of Biotechnology and the Department of Biochemistry and Microbiology. Externally, we work with colleagues from the Institute of<strong> Organic Chemistry and Biochemistry of the CAS (IOCB Prague)</strong> and the <strong>Central European Institute of Technology in Brno (CEITEC)</strong>. Our research naturally necessitates the integration of diverse fields of expertise; it requires combining virology, cell biology, and biochemistry with advanced imaging and modern approaches to study RNA-protein interactions. This is exactly what makes our project capable of addressing complex questions that a single laboratory cannot tackle on its own.</p><h5><strong>Are there plans to continue the project? Have you brought on new collaborators, and are you looking to welcome new colleagues to the team?</strong></h5><p>Yes, definitely. In basic research, it often happens that one answered question opens up several more. High-quality, properly interpreted data provide a foundation you can build on — and that is what moves scientists forward. It is already clear that the topic of retroviral RNA export and packaging has broader implications and may reveal more general principles of how RNA functions within the cell. We also hope the project will be attractive to students, as it connects classical molecular biology, biochemistry, and virology with cutting-edge biotechnological methods. Students are already involved in the project as part of their bachelor’s and master’s theses.</p><h5><strong>What was the personal turning point that motivated you to pursue a long-term investigation into retroviral particles?</strong></h5><p>I first encountered retroviruses in the early 1990s during my diploma thesis, when I was working at IOCB Prague on HIV-1 protease inhibitors. At that time, AIDS could not yet be effectively treated, and retrovirus research had a completely different dimension. From the very beginning, I was fascinated by how such a relatively small and simple virus can outsmart evolutionarily highly optimized cellular processes and turn them to its own advantage. And that has never stopped fascinating me. From a scientific point of view, retroviruses are remarkable; just consider one of their enzymes, reverse transcriptase, without which no diagnostic or research laboratory in the world could function today. Without it, it would be impossible to diagnose RNA viruses like SARS-CoV-2, influenza, or HIV using RT-PCR tests, or, for example to produce recombinant human proteins in bacterial cells.</p>

Tracking unspliced RNA: Professor Rumlová studies how retroviruses “hack” cells

News from LabRulezLCMS Library - Week 20, 2026

<p><strong>🎤Lindsay Repka&nbsp;</strong></p><p><strong>Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.</strong></p><p>In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.</p><p>What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.</p><p>This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.</p><p><strong>If you work in:</strong></p><ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul><p>…this conversation will resonate.</p><h4><strong>Key Topics Covered</strong></h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3><strong>Video Transcription</strong></h3><p>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.</p><h4><strong>What is photoredox chemistry?</strong></h4><p>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.</p><p>Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.</p><h4><strong>Photocrosslinking and drug-target interactions</strong></h4><p>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.</p><p>Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.</p><h4><strong>From photocrosslinking to solvent activation</strong></h4><p>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.</p><p>The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.</p><h4><strong>Why LC-MS was critical</strong></h4><p>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.</p><p>Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.</p><h4><strong>Photocatalyst stability workflow</strong></h4><p>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.</p><p>Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.</p><h4><strong>Quantifying catalyst remaining by LC-MS</strong></h4><p>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.</p><p>Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.</p><p>Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.</p><h4><strong>Why GC-MS was used later</strong></h4><p>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.</p><p>Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.</p><h4><strong>Practical lessons from the project</strong></h4><p>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.</p><p>She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.</p><h4><strong>Final perspective</strong></h4><p>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

Article

Application

Video

Scientists

Interview

Science and research

The University of Chemistry and Technology, Prague is a natural centre of first-rate study and research in the area of chemistry in Czechia and is one of the country's largest educational and research institutions focused on technical chemistry, chemical and biochemical technologies, material and chemical engineering, food chemistry, and environmental studies.

UCT Prague

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LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Agilent Technologies

Phenomenex

Thermo Fisher Scientific

SCIEX

Waters Corporation

<h5><strong>Key Learning Points:</strong></h5><ul><li>Understand best practices upon opening a new LC Column</li><li>Learn how to clean and regenerate an LC Column</li><li>Find out about additional products and practices that can be used to minimize contamination getting onto LC columns</li></ul><h5><strong>Who Should Attend:</strong></h5><ul><li>Any LC user who would like to ensure that their LC columns are properly cared for to make sure to get most life out of it that is possible.</li></ul><blockquote><p><strong>Presenter:</strong> Genevieve Hodson (Senior Manager, Technical Support &amp; Applications, Phenomenex)</p><p>Genevieve graduated from the University of Texas with a BS in Chemistry located in her home town of Austin Texas. She spent 2 years of her undergraduate performing research in an Organic Chemistry Lab synthesizing intermediates. Out of school she spent 4 years as an Analytical Chemist in the QC and R&amp;D Department of Cerilliant (a Sigma Millipore Company). There she worked with many analytical instruments but became an expert at LC by performing purity analysis, customer specific methods, validations, qc testing and stability studies on a wide range of small molecules including pharmaceuticals, pesticides, explosives and illegal substances. From there she moved to Los Angeles California where she worked at Johnson and Johnson and then a small dietary supplement company. After Genevieve spent a year living abroad in Israel raising her first son, she moved back to LA where she got hired as a Technical Specialist at Phenomenex. At Phenomenex she enjoys continuing to learn about chromatography every day through helping customer with all their chromatography needs!</p></blockquote>

Phenomenex: How to Take Care of Your LC Column

How to Take Care of Your LC Column

Learn about the best practices upon opening your new LC Column, how to clean and regenerate, and additional products and practices...

<p><strong>Measuring scattering samples with UV-VIS-NIR can be challenging for basic instruments. These samples will have diffuse reflectance or transmission and less light hitting your detector.</strong></p><p>In this presentation, we will discuss diffuse reflectance accessories (DRAs) for collecting all light in a measurement as well as Agilent's universal measurement accessory (UMA) for measuring the angular distribution of the reflected light.</p><blockquote><p><strong>Presenter:</strong> Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)</p><p>Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.</p></blockquote>

Agilent Technologies: Measuring Diffuse Powders and Films With UV-VIS-NIR

Measuring Diffuse Powders and Films With UV-VIS-NIR

We will discuss diffuse reflectance accessories (DRAs) as well as Agilent's universal measurement accessory (UMA).

<div><h4><strong>2026 AQbD Symposium Day 1 - Pharma Small Molecules</strong></h4><p>Staying ahead of regulatory change is a challenge. Evolving guidelines like ICH Q14, Q2(R2), USP &lt;1220&gt;, and the upcoming ICH M4Q(R2) and USP &lt;1225&gt; are reshaping expectations for analytical methods. Risk management and fit-for-purpose principles are now critical for compliance. Join us to explore how Analytical Quality by Design (AQbD) can help you design robust methods, streamline transfers, and meet today’s stringent small molecule and biopharmaceutical requirements.&nbsp;</p><h5><strong>Why Attend:</strong></h5><ul><li>Learn how to apply AQbD principles to the development of robust, risk-based analytical procedures.</li><li>Use DoE tools to identify risks during method transfer and migration.</li><li>Gain practical insights from industry experts on case studies to improve analytical testing reliability.</li></ul><h4><strong>Session 1:</strong></h4><h5><strong>Pharma Small Molecules Presentations Practical elements from ICHQ14, Q2R2 and USP Enhanced Approach » and Practical Tools to Reduce Analytical Risks&nbsp;</strong></h5><ul><li>Gérald de Fontenay, Scientific Director, CEBIPHAR</li></ul><h5><strong>AQbD and DOE Across Pharma Analytical Development</strong></h5><ul><li>Davide Messini, Analytical Development Senior/Scientist, F.I.S. - Fabbrica Italiana Sintetici S.p.A.</li></ul><h5><strong>AQbD Approaches for GLP-1 RA Impurity Profiling</strong></h5><ul><li>Pawel Bigos, Senior Scientist, Waters Corporation</li></ul><blockquote><p><strong>Presenter:</strong> Gérald de Fontenay (Directeur Scientifique et Technique, CEBIPHAR)</p><p>For more than 25 years, Gérald has held key positions within service companies (analytical CROs and CDMOs). He is currently Scientific and Technical Director at CEBIPHAR, where he ensures the highest possible reliability of the results generated both in CEBIPHAR’s laboratories and in those of its clients.</p><p>He provides his expertise in the validation, verification, and transfer of analytical methods to support CEBIPHAR’s project managers and clients, helping to accelerate the implementation of new projects at production sites and to ensure the compliance of reports.</p><p><strong>Presenter:</strong> Davide Messini (Analytical Development Senior/Scientist, F.I.S. - Fabbrica Italiana Sintetici S.p.A.)</p><p>Degree in Chemistry (Padova). He has more than 20 years of experience in analytical chemistry in active pharmaceutical ingredients (API) manufacturing environment. He started in cGMP quality control as a QC analyst and then as R&amp;D analyst in analytical development department. Since 2010 he is responsible for the analytical development related to the manufacturing of API (small molecules) from lab to commercial scale developing and testing all analytical methods suitable for the quality assessment of the substance. Expertise in all common instrumentation used in pharma environment, he ensures the quality of the analytical methods, analytical development strategy, robustness tests and all related cGMP aspects.</p><p><strong>Presenter:</strong> Pawel Bigos (Senior Scientist, Waters Corporation)</p><p>Pawel Bigos is a Senior Scientist at Waters Corporation in the QA/QC Biologics group in Milford, Massachusetts. He holds a Bachelor of Science degree in Chemistry from the University of South Florida. Prior to Waters, Pawel gained valuable experience at Frequency Therapeutics, Novartis, and Symbiotic Research. Since joining Waters in 2022, he has concentrated on biomolecule separations and plays a critical role in providing analytical support in the development, commercialization, and application of Waters innovative products.</p></blockquote></div>

Waters Corporation: 2026 AQbD Symposium - Day 1 - Pharma Small Molecules

2026 AQbD Symposium - Day 1 - Pharma Small Molecules

Learn how AQbD and new ICH/USP guidelines enhance small-molecule method development, risk control, and lifecycle management at the 2026 AQbD Symposium.

2026 AQbD Symposium - Pharma Small Molecules

<div><h4><strong>Unlock the Secrets of Lipid Nanoparticle Characterization</strong></h4><p><strong>Join our exclusive webinar and discover how to overcome the toughest challenges in LNP analysis.</strong></p><p>Are you working with lipid nanoparticles, liposomes, or other lipid-based drug carriers? Whether you’re in discovery, development, QC, or analytical services, or supporting these efforts in a CRO or biotech institute, this session is for you!</p><h4><strong>What you’ll gain:</strong></h4><ul><li><strong>Boost Lab Efficiency: </strong>Learn how automated zeta potential and size measurements for up to 96 samples in a single run free your team to focus on science, not repetitive tasks.</li><li><strong>Master Challenging Samples: </strong>See how Flow Mode preserves sample integrity and delivers precision, even at physiological ionic strength.</li><li><strong>Simplify Your Workflow:</strong> Experience intuitive guided workflows and DYNAMICS Software, fully 21 CFR Part 11–compliant, to ensure data integrity and smooth transition from research to QC.</li></ul><h4><strong>Why attend?</strong></h4><p>Traditional methods struggle with high-salt media, low-charge samples, and regulatory scrutiny. We’ll show you how the DynaPro ZetaStar solves these pain points, delivering accurate, reproducible results with less than 10% RSD, full compliance, and automation that scales with your needs. Plus, discover how DYNAMICS Software and DYNAMICS Touch make complex analyses simple, reliable, and fast, even from microliter volumes.</p><p><strong>Don’t miss this chance to transform your LNP characterization process.</strong></p><p><i>Reserve your spot today and take the first step toward faster, more reliable insights.</i></p><blockquote><p><strong>Presenter:</strong> Richard Chung (Lab Analytics Product Manager, Waters | Wyatt Technology)</p><p>Since joining Wyatt in 2016, he has held key roles, including Application Scientist and Customer Support Manager, where he worked closely with researchers to solve complex analytical challenges. Now, as a Product Manager, Richard bridges the gap between scientists and better instruments - gathering insights from the field and collaborating with internal research teams to develop cutting-edge solutions that empower laboratories worldwide.</p></blockquote></div>

Waters Corporation: Automation, Speed, and Simplicity for Total Lipid Nanoparticle Characterization

Automation, Speed, and Simplicity for Total Lipid Nanoparticle Characterization

Join our exclusive webinar and discover how to overcome the toughest challenges in LNP analysis.

Total Lipid Nanoparticle Characterization

<h4><strong>How about a 30 minute coffee break along with a taste of Empower?</strong></h4><h4><strong>Here you go!</strong></h4><h5><strong>Short session, big results</strong></h5><p>Learn quick, impactful tricks to make your Empower Software reports smarter and more insightful. Whether you’re aiming for compliance or clarity, these practical hacks will help you save time and elevate your reporting. A short session – for big results.</p><h5><strong>What will you learn?</strong></h5><ul><li>Save time instantly – Discover techniques that dramatically reduce reporting effort</li><li>Boost report quality – Create clear reports that are more professional and easier to understand</li><li>Stay compliant with ease – Integrate regulatory requirements without stress</li></ul><p><i>Reserve your spot today and make your Empower Software reports shine!</i></p><blockquote><p><strong>Presenter: </strong>Ralf Schröder (Informatics Market Development Manager)</p><p><strong>Presenter:&nbsp;</strong>Carsten Fischer (Informatics Product Marketing, EMEA)</p><p><strong>Presenter:&nbsp;</strong>Ilse Hoes (Solutions Manager, Informatics at Waters Europe)</p></blockquote>

Waters Corporation: Informatics series #30 Empower Reports - Simple & Smart

Informatics series #30 Empower Reports - Simple & Smart

Learn quick, impactful tricks to make your Empower Software reports smarter and more insightful. 

Informatics series #30 Empower Reports 

<p><strong>Accurate mass fundamentals breaks down the barriers of complexity, demystifies misconceptions, and provides helpful tips on how to improve your data quality.</strong></p><p>Whether you already use accurate mass spectrometry and are looking to grow your knowledge or are currently working with nominal mass spectrometry and are thinking about making the switch, this webinar series will show you how accurate mass can simplify your workflow, clarify your data, and give you greater confidence in every result.</p><p>Sign up today to discover the practical advantages of accurate mass spectrometry.</p><h4><strong>Session 2: From theory to practical workflows</strong></h4><ul><li>See how accurate mass integrates into everyday workflows</li><li>Confidently interpret HRMS data</li><li>Build robust methods with automation to simplify complex analyses</li></ul>

Accurate mass fundamentals - From theory to practical workflows

How accurate mass spectrometry works in real workflows. Session 2 focuses on HRMS data interpretation, method robustness, and automation for complex analyses.

Accurate mass fundamentals - From theory to practice

<div><h4><strong>2026 AQbD Symposium Day 2 - Biopharmaceutical</strong></h4><p>Staying ahead of regulatory change is a challenge. Evolving guidelines like ICH Q14, Q2(R2), USP &lt;1220&gt;, and the upcoming ICH M4Q(R2) and USP &lt;1225&gt; are reshaping expectations for analytical methods. Risk management and fit-for-purpose principles are now critical for compliance. Join us to explore how Analytical Quality by Design (AQbD) can help you design robust methods, streamline transfers, and meet today’s stringent small molecule and biopharmaceutical requirements.&nbsp;</p><h5><strong>Why Attend:</strong></h5><ul><li>Learn how to apply AQbD principles to the development of robust, risk-based analytical procedures.</li><li>Use DoE tools to identify risks during method transfer and migration.</li><li>Gain practical insights from industry experts on case studies to improve analytical testing reliability.</li></ul><h4>Agenda</h4><h5><strong>Incorporating Analytical Quality by Design in Early Phase Development of mAbs and ADCs&nbsp;</strong></h5><ul><li>Mark Eggink, Principal Scientist, Analytical Development and Quality Control, Byondis</li></ul><h5><strong>Application of Analytical Quality by Design Principles to the Analysis of Nucleic Acids using an Innovative Dual Gradient Approach</strong></h5><ul><li>Ricardo Gonçalves, Senior Analytical Scientist, Hovione</li></ul><h5><strong>Leveraging AQbD Principles for Efficient Development of a Targeted Assay Method for Routine Monitoring of Mannose-5 Glycans Using Fluorescence Detection</strong></h5><ul><li>Pawel Bigos, Senior Scientist, Waters Corporation</li></ul><blockquote><p><strong>Presenter:</strong> Mark Eggink, Ph.D. (Principal Scientist, Analytical Development and Quality Control, Byondis)</p><p>Mark Eggink, PhD, is Principal Scientist at Byondis, where he is responsible for analytical support for process development, method development and validation, batch release and stability testing of monoclonal antibodies and antibody drug conjugates.</p><p><strong>Presenter: </strong>Ricardo Gonçalves (Senior Analytical Scientist, Hovione)</p><p><strong>Presenter: </strong>Udayabagya (Bud) Halim (Sr. Marketing Analyst, Waters Corporation)</p><p>Bud Halimis a senior marketing analyst at Waters | Wyatt Technology with a PhD in physical chemistry from UCLA. He began his career teaching biochemistry and chemistry and contributing to laboratory manuals before transitioning into the scientific instrumentation industry as an applications scientist. At Waters, he supports researchers in the characterization of peptides, biologics, lipid nanoparticles (LNPs), and viral vectors.</p></blockquote></div>

Waters Corporation: 2026 AQbD Symposium - Day 2 - Biopharmaceutical

2026 AQbD Symposium - Day 2 - Biopharmaceutical

Join Day 2 of the 2026 AQbD Symposium to learn how AQbD enables compliant, risk-based biopharmaceutical analytics under evolving ICH and USP guidelines.

2026 AQbD Symposium - Biopharmaceutical

<div><h4><strong>Transform Lab Automation Connectivity</strong></h4><p>Join our exclusive webinar to discover how the <strong>Waters OneLab™ Secured Deployment Solution</strong> takes you from box to bench.</p><p>Your new <strong>Waters™ Andrew+ Robot</strong> has arrived, and you’re ready to automate right away with no IT support required. With intuitive technology and clear guidance, you’ll simplify setup and be on your path to impact in no time.</p><p>When you’re ready to connect OneLab™ Software with <strong>Empower™, MassLynx™, or waters_connect™</strong>, simply include our IT installation documentation with your request. Your IT partner will be ready to make the connection.</p><p>It’s simple, secure, Windows®-based, and designed to isolate the network connection from Andrew+ Robot, now you can automate with confidence.</p><h5><strong>Learn how OneLab Secured Deployment Solution architecture:</strong></h5><ul><li>Enables cybersecurity and delivers enterprise-ready performance</li><li>Accelerates IT implementation with ready-to-use deployment.</li><li>Supports 21 CFR Part 11 and GxP compliance for regulated workflows.</li><li>Integrates seamlessly with waters_connect, Empower, and MassLynx to optimize productivity.</li></ul><h5><strong>Who should attend?</strong></h5><p>If you work in Pharma or Biopharma as an IT Manager, R&amp;D Scientist, or QA/QC Scientist, this exclusive webinar is designed for you.</p><p><i><strong>Don't miss it.</strong></i></p><blockquote><p><strong>Presenter:</strong> Guillaume Mignard (OneLab Product Marketing Manager, Waters Corporation)</p><p>Guillaume Mignard is Product Designer and Product Owner of OneLab™ Software at Waters Corporation.</p><p>Since joining Waters in 2017, he has led the strategic direction, design, and implementation of OneLab™ Software, a cloud-based platform making lab automation accessible to scientists worldwide.</p><p>Guillaume began his career as a UX/UI designer for business software, pioneering user-centric design when it was still emerging beyond web marketing.</p></blockquote></div>

Waters Corporation: Introducing the Waters OneLab Secure Deployment Solution

Introducing the Waters OneLab Secure Deployment Solution

Join our exclusive webinar to discover how the Waters OneLab™ Secured Deployment Solution takes you from box to bench.

Introducing Waters OneLab Secure Deployment Solution

<h3><strong>Achieve reliable, high-performance single-cell proteomics—every day.</strong></h3><p><strong>Join Jeff Op de Beeck (Thermo Fisher Scientific) for a deep dive into a practical single-cell proteomics workflow designed to deliver exceptional sensitivity, precision, and robustness. This session showcases how the Thermo Scientific™ μPAC™ Neo Plus trap-and-elute setup empowers researchers to perform daily single-cell analysis with unmatched depth and reproducibility.</strong></p><h4><strong>What You Will Learn</strong></h4><ul><li>How to achieve reliable low-flow LC-MS performance suited for single-cell studies</li><li>Advantages of μPAC™ micro Pillar Array columns (50 cm) in high-sensitivity workflows</li><li>Implementing trap-and-elute approaches with the Vanquish™ Neo UHPLC</li><li>Maximizing identification depth and quantitative precision using Orbitrap™ Astral­­™ / Exploris™ systems with FAIMS™ interface</li><li>Practical tips for building a robust, daily-use workflow for ultra-low-sample proteomics</li></ul><h4><strong>Topics Covered</strong></h4><ul><li>Low-flow LC-MS</li><li>Single-cell proteomics</li><li>μPAC Neo Plus trap-and-elute workflow</li><li>Variable-flow nano/capillary LC methods</li><li>High-performance MS with Orbitrap Astral/Exploris + FAIMS</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li><strong>Proteomics scientists and mass spectrometrists</strong> seeking higher sensitivity and throughput in single-cell LC-ESI-MS analysis</li><li><strong>Method developers and LC-MS operators</strong> interested in nano/capillary workflows, trap-and-elute strategies, and precise low-nanoliter injections on the Vanquish Neo UHPLC</li><li><strong>Core facility managers and lab leaders </strong>looking to implement robust, reproducible workflows for daily high-productivity operation</li><li><strong>Single-cell biology researchers</strong> working with limited sample material who need consistent, end-to-end workflows from isolation to data interpretation</li></ul><p>We’ll conclude with a live Q&amp;A session—bring your questions!</p><blockquote><p><strong>Presenter: </strong>Jeff Op de Beeck (Staff Scientist R&amp;D, Thermo Fisher Scientific)</p></blockquote>

Thermo Fisher Scientific: Mastering Single Cell Proteomics: Daily Excellence with μPAC Neo Plus Trap-and-Elute Workflow

Mastering Single Cell Proteomics: Daily Excellence with μPAC Neo Plus Trap-and-Elute Workflow

Learn how to achieve robust, high-sensitivity single-cell proteomics using μPAC Neo Plus trap-and-elute LC-MS with Vanquish Neo and Orbitrap systems.

Mastering Single Cell Proteomics: μPAC Neo Plus Trap

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Take your Chromeleon Instrument Methods to the next level with this focused and practical session. This webinar is specifically designed for Thermo Scientific Chromeleon CDS users, who want to enhance their current skills and gain a better understanding of how to control instrument actions and apply dynamic sequence logic.</strong></p><h4><strong>Topics covered include:</strong></h4><ul><li><strong>Script editor overview:</strong> Understand the basics of writing and editing custom scripts</li><li><strong>Conditional statements:</strong> Learn how to use logic to make conditional decisions during runs and sequences</li><li><strong>Information logging:</strong> Record key instrument and method events in real time for extended audit trail coverage</li><li><strong>Custom sequence variables: </strong>Control sequence and instrument behavior and with user-defined fields to enrich meta data capture</li><li><strong>User-defined programs:</strong> Customize injection actions to support complex workflows</li><li><strong>Automated startup and shutdown: </strong>Create routines to prepare instruments for operation or safely power them down</li></ul><h4><strong>Why Attend:</strong></h4><p>Join this webinar to gain practical skills and expert tips for using Chromeleon CDS more effectively. Whether you’re new or experienced, you’ll learn how to streamline workflows and enhance your analytical instrument methods.</p><p><strong>Cannot attend the live broadcast?&nbsp;</strong></p><p><i>Please register anyway, and we will automatically send you the link to the recording.</i></p><blockquote><p><strong>Presenter: </strong>Peter Zipfell (Product Marketing Manager, Thermo Fisher Scientific)</p></blockquote></div></div></div>

Thermo Fisher Scientific: Get more from your Chromeleon Instrument Methods

Get more from your Chromeleon Instrument Methods

Join this webinar to gain practical skills and expert tips for using Chromeleon CDS more effectively.