<div><p><strong>Air bubbles in an HPLC system can disrupt pumping reliability, cause baseline noise, and result in poor result reproducibility and valve function. To prevent these, proper mobile phase degassing is essential.&nbsp;</strong></p><p>Degassing in HPLC not only ensures stable pumping but also improves sensitivity and accuracy by preventing dissolved oxygen from forming bubbles that interfere with detectors such as UV-VIS, fluorescence, refractive index, or electrochemical. Effective HPLC solvent degassing is therefore a critical step for stable, accurate, and reproducible chromatography.</p></div><div><div><div><div><h3><strong>What is HPLC Solvent Degassing and Why is it Necessary?</strong></h3></div></div></div></div><div><p>When solvents such as water, buffers, acetonitrile, and methanol contact the atmosphere, nitrogen and oxygen dissolve into them. When mixed, their combined air content can exceed the solubility of the mixture, creating a supersaturated condition that leads to outgassing and bubble formation. These bubbles disrupt <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc">HPLC</a> performance by causing unstable baselines, bad peak shape, pump cavitation, retention time shifting, and loss of sensitivity.</p><p>In pumps, air can stop flowing or cause erratic retention times, while in optical detectors, bubbles scatter light and generate noise or false signals. Although it is not necessary to remove all dissolved air, degassing in HPLC lowers gas levels below supersaturation and prevents these issues. Low-pressure mixing systems are especially prone to bubble formation, whereas high-pressure mixing reduces outgassing because solvents blend under elevated pressure.</p></div><div><div><div><div><h3><strong>Types of Degassing Techniques in HPLC</strong></h3></div></div></div></div><div><p>Degassing the mobile phase was once a major concern in liquid chromatography with outgassing problems being among the most common issues during the first decades of its use. The introduction of inline degassers in HPLC in the past 15–20 years has significantly reduced the occurrence of these problems, to the point that many users today have little experience with them. However, while inline degassing prevents most solvent outgassing issues, it does not eliminate all problems caused by dissolved air.</p></div><div><div><div><div><h4><strong>Inline Degassing</strong></h4></div></div></div></div><div><p>Inline degassing works by passing the mobile phase through a gas-permeable membrane within a vacuum chamber, allowing dissolved gases to escape while retaining the liquid. Different techniques include:</p><ul><li><strong>Vacuum degasser:</strong> Inline vacuum degassers, now standard in most modern HPLC systems, have greatly reduced bubble-related pump issues by efficiently removing dissolved gases. Solvents pass through porous tubing within a vacuum chamber, where gases diffuse out through micropores to waste, while the degassed liquid continues flowing onto the pump.</li><li><strong>Membrane degasser:</strong> Over the years, various polymer materials have been tried with varying success for degassers. Modern degassers use a thin, highly gas-permeable membrane, making the degassing process very efficient.</li></ul></div><div><div><div><div><h5><strong>Advantages</strong></h5></div></div></div></div><div><p>Inline degassers are highly reliable and effective at reducing dissolved gases in the mobile phase to acceptable levels and are now standard in most HPLC systems. It is usually automatic and trouble-free. They require little to no maintenance, making them convenient and reliable for routine use.</p></div><div><div><div><div><h5><strong>Limitations</strong></h5></div></div></div></div><div><p>Despite their effectiveness, inline degassers do not remove all dissolved air from the mobile phase. The residual air can still cause problems in certain sensitive applications. Membrane replacement, vacuum pump failure, or damaged tubing can also cause issues, so proper maintenance is essential to avoid costly repairs. Additionally, microbial growth in the buffer can block membrane pores and contaminate the entire HPLC system. To prevent microbial growth, contamination, or blockages, it is best not to store the degasser with water or buffer in the tubing and to flush with 30% phosphoric acid followed by water if contamination occurs.</p></div><div><div><div><div><h4><strong>Offline Degassing</strong></h4></div></div></div></div><div><p>Offline degassing involves removing dissolved air from the mobile phase before it enters the HPLC system. The techniques include:</p><ul><li><strong>Helium sparging:</strong> This method requires a solvent inlet filter to prevent particulates and improve gas dispersion, but the degassed solvent can reabsorb air once in the system. To reduce bubble formation, slight back pressure should be maintained, often using a backpressure regulator after the detector. Helium purging can remove <strong>up to 80% of dissolved air</strong>, requiring equal helium volume for organic–aqueous phases. However, the flow rate should be reduced over time to avoid losing volatile components.</li><li><strong>Vacuum filtration:</strong> Vacuum degassing removes <strong>over 60%</strong> of dissolved air from the mobile phase and is performed through batch-wise treatment. It can also be done during filtration, before the chromatographic process with a 0.45 or 0.22 μm membrane filter, though this method is less effective.</li><li><strong>Sonication:</strong> Ultrasonic baths alone are not effective for degassing, as they remove<strong> only about 20-30% of dissolved gases</strong>, while at least 50% removal is needed. However, when combined with helium sparging or vacuum degassing, sonication becomes a <strong>useful complementary method</strong>. Since ultrasonic baths are common in laboratories, they are best applied alongside other degassing techniques.</li></ul></div><div><div><div><div><h5><strong>Advantages</strong></h5></div></div></div></div><div><p>An offline degasser offers the advantage of using a 2 µm solvent inlet filter to prevent particulates from entering the mobile phase while ensuring efficient gas dispersion. It is also cost-effective and flexible, as it relies on standard laboratory equipment and can handle large solvent volumes in a batch.</p></div><div><div><div><div><h5><strong>Limitations</strong></h5></div></div></div></div><div><p>A major drawback of offline helium sparging is that the mobile phase can gradually reabsorb air once placed in the HPLC system, leading to signal loss and changing sensitivity. Additionally, helium sparging can be expensive as it requires specialized gas cylinders.</p><h3><strong>Inline vs Offline Degassing: Side-by-Side Comparison</strong></h3><h5><strong>Timing:</strong></h5><ul><li><strong>Offline Degassing: </strong>It is usually done before the mobile phase enters the HPLC run.</li><li><strong>Inline Degassing: </strong>It is a continuous process that occurs during an HPLC run.</li></ul><h5><strong>Degassing Efficiency:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>The efficiency can be variable. For instance, vacuum/sonication removes 60–70% of dissolved air, sonication alone removes <strong>20–30%</strong> of air and helium sparging removes 80% of air.</li><li><strong>Inline Degassing:&nbsp;</strong>It does not remove 100% of dissolved gas, but it removes most of it. However, it prevents bubble formation.</li></ul><h5><strong>Risk of Re-dissolving:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>The risk is high as solvents can reabsorb air when exposed to the atmosphere.</li><li><strong>Inline Degassing:&nbsp;</strong>The risk is low as the continuous vacuum keeps new gas from dissolving.</li></ul><h5><strong>Cost:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Low initial investment and requires only basic lab equipment. <strong>Helium</strong> sparging can be expensive as it requires specialized gas cylinders.</li><li><strong>Inline Degassing:&nbsp;</strong>Higher initial cost (dedicated degasser unit and vacuum pump).</li></ul><h5><strong>Ease of Use:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Manual setup each time which is time-consuming.</li><li><strong>Inline Degassing:&nbsp;</strong>Automated after installation.</li></ul><h5><strong>Impact on Solvent Composition:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Some loss of volatiles is possible such as evaporation during sparging.</li><li><strong>Inline Degassing:&nbsp;</strong>Solvent composition remains unchanged.</li></ul><h5><strong>Effect on Chromatographic Stability:</strong></h5><ul><li><strong>Offline Degassing:&nbsp;</strong>Improves initial stability but may allow bubble re-formation later.</li><li><strong>Inline Degassing:&nbsp;</strong>Very stable baselines and retention. Bubble-related problems are virtually eliminated.</li></ul><div><div><div><div><h3><strong>When to Choose Offline vs Inline Degassing</strong></h3></div></div></div></div><div><p>The choice between an offline and inline HPLC degasser depends on the situation, including factors like routine use, troubleshooting needs, and method sensitivity.</p><ol><li>Offline degassing is suitable for occasional use, limited equipment setups, or when routine inline degassing is not available. While inline degassing is preferred for routine analyses requiring consistent performance and high sensitivity.</li><li>Inline degassers have a higher initial cost but save labor. An offline degasser uses low-cost labware and is cheap to set up, though helium or other gases add to the consumable cost.</li><li>If mobile phases contain volatile organics or labile salts, avoid aggressive offline sparging/vacuum (which can evaporate components). Inline vacuum degassing is gentler on the solvent composition.</li><li>Oxygen in the mobile phase is particularly problematic for sensitive methods, such as low-wavelength UV or reductive electrochemical detection. While techniques like helium sparging and inline degassing effectively remove oxygen, remember that offline-sparged solvents are susceptible to reabsorbing air over time, which can lead to signal loss and subtle changes in sensitivity.</li></ol></div><div><div><div><div><h3><strong>Troubleshooting Degassing Issues in HPLC</strong></h3></div></div></div></div><div><h4><strong>1) Cycling of output (pressure or baseline)</strong></h4><p>Cycling patterns in pressure or the chromatogram baseline often indicate poor compressibility at the pump heads. This may result from insufficient degassing efficiency, where adding a second degassing chamber can help. It can also cause air to enter the system due to improperly connected tubing to or from the HPLC solvent degasser. Also, improper connections of the tubing on the HPLC solvent degasser can also allow air to enter the system, causing fluctuations in pressure or baseline.</p><h4><strong>2) Baseline noise</strong></h4><p>Noise on the baseline of a chromatogram may indicate problems with the gas flow system. High gas flow rates can cause short-term noise, while baseline drift (long-term fluctuations) may be due to contaminants being flushed out from a partially clogged gas line. Additionally, an unstable gas flow output can also contribute to baseline noise.</p><h4><strong>3) Leakages</strong></h4><p>Leaks often occur due to incompatibility between solvents and degasser materials. For instance, tetrahydrofuran (THF) can dry and crack degasser membranes. Additionally, improperly seated tubing connections in or out of the degasser can allow leaks to develop.</p><h4><strong>4) Blockages</strong></h4><p>Blockages create pressure ripples as the pump struggles to draw solvent, sometimes causing cavitation. They may result from algae growth, crystallization of buffer salts, or clogged inlet filters. Troubleshooting involves disconnecting outlet tubing to check for continuous solvent flow, while prevention requires filtering solvents, using clean solutions, and flushing lines regularly, especially after system shutdowns.</p><h4><strong>5) General considerations</strong></h4><p>Degasser issues are often linked with the mobile phase quality. Using poorly prepared or contaminated solvents can lead to persistent problems. Maintaining clean, filtered, and fresh solvents helps ensure smooth operation and minimizes downtime.</p></div></div>

Degassing Techniques in HPLC: Inline vs Offline

Everything to know about osmolality

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 13th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you posters by <strong>Agilent Technologies / ASMS, Shimadzu / ASMS, Thermo Fisher Scientific / HPLC Symposium </strong>and<strong> Waters Corporation / HPLC Symposium </strong>and technical note by<strong> KNAUER!</strong></p><h3><strong>1. Agilent Technologies / ASMS: Intact Protein Analysis in Plasma Using Automated 2D-LC/TOF with Multiple Heart-Cutting</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_intact_protein_mhc_2d_lc_tof_wp_679_asms_2026_en_agilent_edb54f4854.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Direct intact and top-down protein analysis enables comprehensive characterization of proteoforms, post-translational modifications, and fragments that are often lost in bottom-up workflows.1 However, intact mass analysis in complex biological matrices such as whole plasma is challenged by extreme dynamic range and ion suppression, commonly necessitating affinity-based enrichment.&nbsp;</p><p>Here, we demonstrate a non-affinity, orthogonal separation strategy using a 2D-LC/TOF workflow with multi-heart-cutting for native intact protein analysis directly in plasma. Therapeutic monoclonal antibodies (mAbs) trastuzumab and antibody-drug conjugate T-DM1, were used as model systems to evaluate performance.&nbsp;</p><p>The workflow employs an Agilent 1290 Infinity III 2D-LC system for orthogonal separations and precise multiple heart-cutting, coupled to a high-resolution, high-sensitivity Agilent 6230C Accurate Mass TOF LC/MS System.</p><h4><strong>Experimental</strong></h4><h5><strong>Instrumentation&nbsp;</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III+2D-LC"><strong>Agilent 1290 Infinity III 2D-LC system </strong></a>comprised the following modules:&nbsp;<ul><li>1290 Infinity III Bio Multisampler with Thermostat (G7137A)&nbsp;</li><li>1290 Infinity III Multicolumn Thermostat (G7116B)&nbsp;</li><li>1290 Infinity III Diode Array Detector (G7117B) with Max-Light cartridge cell (G7117-60020)&nbsp;</li><li>1260 Infinity III Bio Flexible Pump (G7131C)&nbsp;</li><li>1290 Infinity III Bio High-speed Pump (G7132A)&nbsp;</li><li>1290 Infinity Valve Drive (G1170A) with 2D-LC ASM Valve (G5643B)&nbsp;</li><li>Two 1290 Infinity III Valve Drives (G1170A) with multiple heart-cutting valves (G5642-64000) equipped with 40 µL Bio loops&nbsp;</li><li>1290 Infinity III Valve Drive (G1170A) with a 2- position/6-port valve (G4231A) (used as a diverter valve)&nbsp;</li></ul></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6230C"><strong>Agilent 6230C Accurate Mass TOF LC/MS System</strong></a></li></ul><h5><strong>LC/MS Analysis&nbsp;</strong></h5><p>Data acquisition and analysis were carried out using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=MassHunter+acquisition"><strong>MassHunter acquisition</strong></a> (12.2), <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=BioConfirm"><strong>BioConfirm</strong></a> (12.1) and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=MassHunter+Qualitative+Analysis"><strong>MassHunter Qualitative Analysis </strong></a>(13.0) software.</p><h4><strong>Conclusions</strong></h4><ul><li>Agilent 2D-LC enables direct native MS analysis of intact proteins from plasma without affinity-based enrichment.&nbsp;</li><li>UV-guided and multiple heart-cutting simplify 2D-LC method development and provide high-resolution isolation of native proteins from complex biological matrices such as plasma.&nbsp;</li><li>The Agilent 6230C Accurate Mass TOF delivers high sensitivity and excellent mass accuracy, enabling confident intact protein characterization under native conditions.</li></ul><h3><strong>2. KNAUER: Mind the pressure in preparative up-scaling – effects of particle size and solvent composition on back pressure</strong></h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0051_knauer_mind_the_pressure_in_preparative_up_scaling_effects_of_particle_size_and_solvent_comp_12e49884d3.pdf"><strong>Full PDF for download</strong></a></li></ul><p>The back pressure of a chromatographic system is generated by the system components, the capillaries, the column, the solvent, and the applied flow rate. Each system has a maximum pressure which it can sustain, and therefore the pressure must be monitored as it is a limiting factor during method development. The back pressure generated by a column is dependent on the particle size and its length. The development of a preparative method is ideally performed on an analytical scale and after optimization the method is then scaled up to preparative scale.</p><p>As preparative HPLC systems normally have lower maximum pressures, it is important to keep the generated pressure in the range of the later preparative system.&nbsp;</p><p>The larger the particles of the column material are, the lower will be the generated back pressure. With increasing particle size, the resolution is decreasing at the same time. Depending on the separation task, the resolution can be a critical point, and it is important to find the right compromise between resolution and backpressure. Additionally, larger particles are significantly less price intensive, which is another factor to be considered especially for large scale purifications. Often solvent mixtures of water with acetonitrile, methanol or ethanol are used for reverse phased methods. Depending on the organic solvent composition, the generated back pressure differs significantly which could be reduced by changing the solvent composition slightly or the organic solvent if possible. The back pressure generated by the solvent will not or only slightly change during the up-scaling process.&nbsp;</p><p>Here, the influence of particle size, flow rate and solvent composition on the back pressure was evaluated. Further, a practical scale-up was performed to evaluate the values obtained on analytical scale to those in larger scale.</p><h4><strong>User Tips:&nbsp;</strong></h4><ul><li>What is the finale scale you want to operate later?&nbsp;</li><li>What are the pressure limitations of the preparative system?&nbsp;</li><li>Be aware of the solvent back pressure&nbsp;</li><li>Consider particle size not only for best separation&nbsp;</li><li>Consider the flow rate</li></ul><h4><strong>MATERIAL AND METHODS&nbsp;</strong></h4><ul><li><strong>Pump: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2372"><strong>AZURA® P 6.1LHPG</strong></a>, 10 ml, sst&nbsp;</li><li><strong>Autosampler: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2375"><strong>AZURA AS 6.1L</strong></a></li><li><strong>Detector:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2423"><strong>UV AZURA DAD 2.1L&nbsp;</strong></a></li><li><strong>Flow cell: </strong>Pressure proof 10 mm, 10 µl, 300 bar&nbsp;</li><li><strong>Thermostat:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2376"><strong>CT2.1&nbsp;</strong></a></li><li><strong>Columns:&nbsp;</strong><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1633"><strong>Eurospher II 100-5 C18, 150 x 4.6 mm&nbsp;</strong></a></li><li>Eurospher II 100-10 C18, 150 x 4.6 mm&nbsp;</li><li>Eurospher II 100-15 C18, 150 x 4.6 mm&nbsp;</li><li>Eurospher II 100-20/45 C18, 150 x 4.6 mm &nbsp;</li></ul></li><li><strong>Software&nbsp;</strong><ul><li>ClarityChrom 10.1&nbsp;</li><li>ClarityChrom 10.1 PDA extension</li></ul></li></ul><h4><strong>CONCLUSION&nbsp;</strong></h4><p>The back pressures of four C18 150 x 4.6 mm columns, which differed only in particle size were measured and compared at different flow rates and solvent compositions. As expected, the 5 µm column showed the highest and the 20/45 µm column the lowest back pressure at all tested conditions. The pressure curves of ethanol and methanol gradients were similar, with a maximum pressure at approximately 50% solvent. The pressure curve of the acetonitrile gradient differed from the other two, showing the highest pressure at a lower organic solvent content of approximately 35%, after which the pressure dropped. Ethanol showed the highest pressure, followed by methanol and then acetonitrile. Determining the column back pressure at an analytical scale is an important tool during preparative method development. Ideally, if a linear scale up from the analytical to the preparative scale is conducted, the column back pressure should stay the same. This helps to predict if the method can be scaled up to the desired size in terms of back pressure.</p><h3><strong>3. Shimadzu / ASMS: Enhanced Quantification of Emerging UV Filter Contaminants in Drinking Water Using Optimized LC-MS/MS Technique</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/TP_121_Enhanced_Quantification_of_Emerging_UV_Filter_Contaminants_in_Drinking_Water_Using_Optimized_LC_MSMS_Technique_e733c64828.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Ultraviolet (UV) filter chemicals from sunscreens and personal care products are increasingly recognized as emerging contaminants of concern in drinking water1 . Their persistence and reported biological effects highlight the need for sensitive and reliable monitoring approaches2 . The objectives were to optimize quantitation to achieve ppt level detection without offline extraction and assess the occurrence of these compounds in multiple drinking water matrices. In this study, 20 UV filters were analyzed using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8060RX"><strong>Shimadzu LCMS8060RX triple quadrupole mass spectrometer.</strong></a></p><h4><strong>Conclusion</strong></h4><p>A highly sensitive, precise, and robust direct injection LC–MS/MS method enables sub-ppt to high-ppb quantification of 20 UV filters in drinking water. Overall, the highly sensitive and robust Shimadzu LCMS-8060RX Triple Quadrupole Mass Spectrometer combined with this extraction-free workflow provides a simple, reliable, and high-throughput approach for quantifying emerging UV filters at ng/L concentrations in drinking water.</p><h3><strong>4. Thermo Fisher Scientific / HPLC Symposium: Comparison of biocompatible and inert UHPLC systems for LC-UV quantitation of large RNA</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004752_hplc_vanquish_amplify_metal_sensitive_analyte_analysis_po004752_na_en_ff99cfde49.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Accurate LC-UV analysis of large RNA and oligonucleotide therapeutics requires highly inert chromatographic flow paths because interactions between the negatively charged phosphodiester backbone and metallic surfaces can cause analyte loss, peak broadening, and reduced quantitative sensitivity, particularly at low concentration.&nbsp;</p><p>Here, an inert UHPLC system – Vanquish Amplify system, incorporating inert-coated flow path components was evaluated against a biocompatible UHPLC configuration using MP35N alloy capillaries and additionally against a commercially available inert UHPLC platform. Large RNA fragments of 500 and 5000 nucleotides were analyzed by IP-RPLC to assess the difference between these systems. To further investigate flow path interactions, adenosine 5′- (α,β-methylene) diphosphate (AMPcP), a metal-sensitive analyte, was analyzed alongside adenosine, a noninteracting reference compound.</p><h5><strong>Material and Methods</strong></h5><ul><li><strong>Instrumentation: </strong>Thermo Scientific Vanquish Amplify UHPLC system</li><li><strong>Column:</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=3&amp;search=SurePac+Oligo"><strong>Thermo Scientific SurePac Oligo RP MDi HPLC column</strong></a>,<br>2.5 µm 2.1 X 50 mm (P/N: 43712-052132</li><li><strong>Data Analysis: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>The Thermo Scientific Chromeleon Software 7.3.2 MUc Chromatography Data System (CDS)</strong></a> was used for all data acquisition and analysis.</li></ul><h4><strong>Conclusions&nbsp;</strong></h4><p>Using AMPcP with the Vanquish Amplify system demonstrated up to a twofold increase in sensitivity compared to systems with MP35N fluidics under conditions where metal interactions are most pronounced&nbsp;</p><p>For large RNA, the Vanquish Amplify UHPLC system delivered 1.25–1.35× higher signal intensity compared to systems with MP35N fluidics, enabling reduced sample consumption and improved quantitative reliability.&nbsp;</p><p>The Vanquish Amplify UHPLC system demonstrated comparable system inertness under the tested conditions for larger RNA fragments (5,000 nt) and slightly better system inertness for smaller fragments (500 nt) in this study than the MaxPeak HPS containing system, based on peak height and area comparisons using an external UV detector</p><h3><strong>5. Waters Corporation / HPLC Symposium: Development and Transfer of Quantitative Analytical Methods using Universal Detectors</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009493_8cbe4c3cc2.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>Universal detectors such as <strong>Charged Aerosol Detection (CAD)</strong> and <strong>Evaporative Light Scattering Detection (ELSD)</strong> provide valuable alternatives to traditional LC detectors including UV, fluorescence, and mass spectrometry, particularly for analytes lacking chromophores. Although both detectors are suitable for quantitative analysis, they rely on different detection principles—CAD measures the electrical charge transferred to aerosol particles, whereas ELSD detects light scattered by dried analyte particles. Because both detectors exhibit non-linear responses, quantitative methods typically require calibration models such as log-log linear or quadratic regression rather than conventional linear calibration. The poster discusses key considerations for developing robust quantitative methods and highlights the challenges associated with transferring methods between different universal detection platforms.</p><p>To demonstrate detector transferability, the authors migrated a <strong>HILIC method for sugar analysis in fruit juice</strong> from ELSD to CAD. The separation was performed on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/306"><strong>Waters Arc HPLC System</strong></a> equipped with an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=XBridge+BEH+Amide"><strong>XBridge BEH Amide</strong></a><strong> (4.6 × 150 mm, 2.5 μm)</strong> column using a water/acetonitrile gradient containing triethylamine. While the chromatographic conditions remained unchanged, CAD detector parameters were optimized. Both detectors produced highly comparable quantitative performance across a calibration range of <strong>50–1000 μg/mL</strong>, achieving calibration coefficients (R²) above <strong>0.995</strong>, area RSD values below <strong>1.5%</strong>, and nearly identical concentrations of fructose, sorbitol, glucose, and sucrose in an apple juice sample. These results demonstrate that quantitative HILIC methods can be successfully transferred between ELSD and CAD with only detector-specific optimization.</p><p>The poster also evaluates method transfer between different CAD platforms using the <strong>USP Deoxycholic Acid assay</strong>. The method was implemented on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/198"><strong>Waters ACQUITY UPLC H-Class PLUS System</strong></a> with an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1823"><strong>XBridge BEH C18</strong></a><strong> (4.6 × 150 mm, 3.5 μm)</strong> column and compared against a competing LC/CAD system. After scaling the method according to <strong>USP Chapter &lt;621&gt;</strong>, both systems met all USP system suitability requirements, including repeatability and signal-to-noise criteria. Quantitative results for deoxycholic acid and related impurities were virtually identical, confirming that validated CAD methods can be reliably transferred between different vendors' LC/CAD platforms without compromising analytical performance.</p><p>Overall, the study demonstrates that both ELSD and CAD are robust universal detection techniques for quantitative liquid chromatography. While method transfer between identical detector types is generally straightforward, successful migration between ELSD and CAD—or between different CAD systems—requires careful optimization of detector parameters while preserving chromatographic conditions. The presented workflows illustrate that with appropriate calibration and detector tuning, laboratories can achieve equivalent sensitivity, precision, and quantitative accuracy, facilitating method standardization and transfer across analytical platforms.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 29, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Waters Corporation

<p><strong>ADCs represent complex biologics combing a targeting antibody molecule with a cytotoxic warhead via a linker system. The design of the linker systems is crucial to ensure the warhead is only released at the target site, whilst enabling conjugation to an active site on the protein.</strong></p>
<p>Mass spectrometry can be utilized in various modalities to monitor loss of payloads when incubated in plasma providing a rapid measurement of relative stability.</p>
<p>This webinar will detail how mass spectrometry is used in ADC development and how the X500B QTOF system represents a versatile platform for measurement of intact, reduced, and digested proteins.</p>
<p>Attend this webinar to:</p>
<ul>
<li><strong>Understand how LC-MS can be used to study the stability of an ADC in plasma</strong></li>
<li><strong>Find out how LC-MS can be used to calculate the antibody drug ratio of an intact ADC</strong></li>
<li><strong>Discover how LC-MS has been used to study the payload release of an ADC</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Chris Nortcliffe (Mass Spectrometry Manager | Sterling Pharma Solutions | UK)</p>
</blockquote>
<blockquote>
<p>Dr Christopher Nortcliffe is the mass spectrometry manager in the UK at Sterling Pharma Solutions, a CDMO pharmaceutical partner company. He has a background in protein and DNA analysis, obtaining his PhD from the University of Manchester on studying noncovalent interactions by mass spectrometry in 2016. After Manchester, Christopher worked for SCIEX as a specialist in LC-MS analysis of biologics before moving to Sterling Pharma Solutions, where he manages the LC-MS service and is an expert in the LC-MS analysis of biotherapeutics and ADCs.</p>
</blockquote>


SCIEX: Use of mass spectrometry to assess plasma stability of Antibody Drug Conjugates

Use of mass spectrometry to assess plasma stability of Antibody Drug Conjugates

How mass spectrometry is used in ADC development and how the X500B QTOF system represents a versatile platform.

Use of mass spectrometry to assess plasma stability of ADCs

<p><strong>Tangential flow filtration (TFF), also known as cross-flow filtration, is a rapid and efficient method for the separation and purification of biomolecules.</strong></p>
<p>TFF can be used to concentrate sample solutions ranging in volumes from 10 mL to thousands of liters. It can also be used to fractionate large from small biomolecules, harvest cell suspensions and clarify fermentation broths and cell lysates.</p>
<p>Another major usage of TFF is to perform a process called diafiltration, a technique that uses ultrafiltration membranes to completely remove, replace or lower the concentration of salts or solvents from solutions containing proteins, peptides, nucleic acids and other biomolecules.</p>
<p>This webinar will explore the applications of diafiltration across immunology, protein chemistry, molecular biology, biochemistry and biotechnology.</p>
<p>Attend this webinar to learn:</p>
<ul>
<li>How to optimize laboratory-scale protein purifications</li>
<li>About tangential flow filtration techniques and ultrafiltration membrane selection</li>
<li>How to operate both continuous and discontinuous diafiltration methods</li>
</ul>
<p><strong>Presenter:</strong> Richard Pearce (Independent Consultant)</p>


Technology Networks: Simplified Diafiltration for Protein Purification

Simplified Diafiltration for Protein Purification

This webinar will explore the applications of diafiltration across immunology, protein chemistry, molecular biology, biochemistry and biotechnology.

<p><strong>LC-analogue detection has become widely accepted as a core analytical tool in many laboratories.  This method of detection affords itself to a diverse range of applications supporting routine analysis. Sometimes LC-UV generated data is simply not enough, and orthogonal detection techniques like LC-MS will add more information for complete sample characterisation enabling more confident and faster results.</strong></p>
<p>This webinar focuses on:</p>
<ul>
<li><strong>Why is LC-MS a complementary technique to optical detection?</strong></li>
<li><strong>Single quadrupole mass spectrometry is a well-respected, robust and reliable technique</strong></li>
<li><strong>Easy adoption into undergraduate and graduate teaching and research laboratories</strong></li>
</ul>
<p>Key learning objectives:</p>
<ul>
<li><strong>What is single quadrupole mass spectrometry?</strong></li>
<li><strong>Benefits to laboratories of adopting this technique and perceived barriers</strong></li>
<li><strong>Practical tips for coupling single quadrupole MS to liquid chromatography</strong></li>
<li><strong>Examples of how mass spectral data has helped to solve real analytical problems</strong></li>
</ul>
<p>Who should attend?</p>
<ul>
<li>Scientists in pharmaceutical, chemical, clinical, contract research organizations (CRO), cosmetics and agrochemical industries, universities, and governmental laboratories</li>
<li>Chromatographers wanting to transition to LC-MS from traditional LC-UV workflows</li>
<li>Anyone with an interest in mass spectrometry who wants to learn more</li>
</ul>
<p> The session will conclude with a live Q&amp;A where the team will be on hand to answer all of your questions.</p>
<blockquote>
<p><strong>Presenter:</strong> Professor Graham John Langley PhD BSc CChem CSci FRSC (Director of SCAS and Head of the Mass Spectrometry Facility, University of Southhampton)</p>
</blockquote>
<blockquote>
<p>Professor John Langley is an internationally recognized leader in chromatography - mass spectrometry (MS) with &gt;35 years’ experience and has led and managed the MS/chromatography-MS facility at Southampton Chemistry since 1988.</p>
</blockquote>
<blockquote>
<p>John has &gt;120 publications and has delivered innovative approaches to MS and chromatography-MS, e.g. the first academic open access MS instrumentation in 1995, open access LC-MS, GC-MS and then SFC-MS in 2014.</p>
</blockquote>
<blockquote>
<p>He is a Fellow of the Royal Society of Chemistry (RSC), Chartered Chemist, Chartered Scientist, Member of RSC Analytical Division Council (2014-20), Chair of the RSC Separation Science Group (2009-) and Past President of the International Mass Spectrometry Foundation (2018-22). He is the only person to have been Chair of the BMSS and the RSC Separation Science Group, and was presented with the British Mass Spectrometry Society medal in 2021.</p>
</blockquote>
<blockquote>
<p>He is director of the SW Regional GCxGC-HRMS facility, and the equivalent SW Regional LCxLC-IMS-HRMS facility.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Cristian Cojocariu (Program Lead – Routine Mass Detection, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Cristian Cojocariu (Program lead – Routine Mass Detection) is an experienced scientist with a demonstrated history of working with leading analytical instruments vendors. At Waters, Cristian is responsible for leading the routine mass detection program which provides globally recognized scientific leadership in the area of mass detection measurement in the life, food, and materials sciences.</p>
</blockquote>
<blockquote>
<p>In both the academic and industrial settings, Cristian has acquired extensive experience with GC-MS and LC-MS systems used to examine organic chemicals in biological, food, materials, and environmental samples. He holds a Bachelor of Science degree in Biology and a PhD in Biosphere/Atmosphere interactions from Albert Ludwigs University of Freiburg, Germany.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Vicky Starkie (Principal Marketing Manager – Routine Mass Detection, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Vicky Starkie - Principal Marketing Manager – Routine Mass Detection graduated from Manchester Metropolitan University (UK) with a BSc (Hons) degree in Applied Chemistry. Vicky studied part-time to gain her qualifications whilst working in industry resulting in over 25 years’ experience in a range of analytical techniques including LC, LC-MS, and LC-MS/MS.</p>
</blockquote>
<blockquote>
<p>For the last 8 years Vicky has been working at Waters in a variety of field-based roles supporting customers, providing pre and post-sales application support. In January 2021 Vicky joined the product management and marketing team and is passionate about delivering mass detection solutions to support customers in life, food, and materials sciences.</p>
</blockquote>


Waters Corporation: Routine LC-MS Mass Detection Made Easy

Routine LC-MS Mass Detection Made Easy

Benefits of adopting routine mass detection for traditional LC-UV workflows

<p><strong>As an analytical scientist, you need robust, flexible, and easy solutions to help you characterize and understand your oligonucleotides faster.</strong></p>
<p>Have a closer look at the Waters’ solution:</p>
<p>Exceptional LC-MS performance to maximize sample analysis and comprehensive informatics capabilities, specifically designed for biopharmaceutical analytical laboratories that need to be able to make more confident and more efficient analytical decisions.</p>
<p>Join our 30 Min live session, directly from the Waters Wilmslow Demo Lab:</p>
<ul>
<li><p><strong>Find out how Waters solutions can help you to know more about your oligonucleotides – quicker and easier than ever!</strong></p>
</li>
<li><p><strong>Learn about the benefits of a flexible system and gain insight into this exciting solution and how it can help your lab overcome analytical challenges.</strong></p>
</li>
<li><p><strong>Discover the Waters solution for extended Oligonucleotide analyses, including a preview of the INTACT Mass app and the CONFIRM Sequence app in waters_connect</strong></p>
</li>
</ul>
<p>Waters MS Systems - including ACQUITY Premier LC-System, MaxPeak Premier Columns, and waters_connect Software - are a workflow-based solution: It is easy to implement and operate and will improve decision-making and time to results in your laboratory.</p>
<p><strong>Join Dr. Jo-Anne Riley for this live demo. She will show you how easy it is to set up and operate the Xevo G3 QTof system and introduce you to our INTACT Mass and CONFIRM Sequence workflows from samples to results.</strong></p>


Waters Corporation: Oligonucleotide Analyses using LC-MS: A Live Demo

Oligonucleotide Analyses using LC-MS: A Live Demo

Find out how Waters solutions can help you to know more about your oligonucleotides – quicker and easier than ever!

<p><strong>Don’t miss this exclusive look into how Trilogy Analytical Laboratory, a high-throughput food and feed safety laboratory, relies on robust LC-MS/MS solutions to consistently test for mycotoxins across a multitude of commodities with speed and selectivity.</strong></p>
<p>We’ll look at:</p>
<ul>
<li>How molds and the mycotoxins end up in grain</li>
<li>Differences between testing grain and its byproducts</li>
<li>Improving predictions of mycotoxin occurrence</li>
<li>Delivering trustworthy results to your clients quickly</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Julie Brunkhorst</p>
</blockquote>
<blockquote>
<p>Julie Brunkhorst has been with Trilogy since it opened in 1999 and is currently the Vice-President of the Technical Division. As an essential part of Trilogy’s growth and success, Julie oversees the daily operations of the laboratory and directs all R&amp;D activities. Her expertise in designing procedures customized for high throughput laboratories has enabled Trilogy to reduce run times, and in many cases, offering same-day turnaround. Julie holds a BAS in Chemistry from Maryville University.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Carrie Maune</p>
</blockquote>
<blockquote>
<p>Carrie Maune is President and CEO of Trilogy Analytical Laboratory and is one of the original founders of the organization. Carrie has dedicated 23 years of her professional life to helping build Trilogy into the industry-trusted laboratory that it is today. Carrie has 35+ years of mycotoxin experience and has a passion for sharing her knowledge with others. Carrie is a graduate of Central Missouri University.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Jordon Bierbaum</p>
</blockquote>
<blockquote>
<p>Jordon Bierbaum is the Vice-President of Operations at Trilogy Analytical Laboratory. Jordon started with the organization in 2009 as a chemist and quickly advanced within the laboratory. Jordon has held management positions within Trilogy’s laboratory and production department and this well-rounded experience give him a unique knowledge of quality processes and quality materials. Jordon has his BS of Science in Medicinal Chemistry from the University of Missouri-Columbia and an MBA from Missouri Baptist University.</p>
</blockquote>


SCIEX: The truths, myths, and intricacies of testing mycotoxins

The truths, myths, and intricacies of testing mycotoxins

How Trilogy Analytical Laboratory relies on LC-MS/MS solutions to consistently test for mycotoxins across a multitude of commodities with speed and selectivity.

<p><strong>During this second session of a four-part ‘Fundamentals of Spectroscopy’ course, we will break down some common spectroscopic techniques (UV/VIS absorption spectroscopy, photoluminescence, and Raman scattering amongst others), examine how they work at a relatively low level, and discuss what they can be used to learn in different situations. We will pay special attention to the historical developments of these techniques, particularly the scientific and economic motivations that drove the people who created and refined them.</strong></p>
<p>Key learning objectives</p>
<ul>
<li><p><strong>Understand the concept of absorption spectroscopy, the design of an absorption spectrometer, and see how UV-VIS absorption spectroscopy can be used in quantifying protein concentration.</strong></p>
</li>
<li><p><strong>Differentiate between the different types of luminescence, specifically photoluminescence vs. fluorescence and understand the basics of fluorescence and phosphorescence spectrometers. Become familiar with applications using time-resolved fluorescence, solvatochromic shifts and Förster resonance energy transfer.</strong></p>
</li>
<li><p><strong>Learn the basics of Raman spectroscopy, and the design of a Raman spectrometer. Understand the units used in Raman spectroscopy and learn how Raman specificity can be used to measure various physical and chemical properties in materials.</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter</strong> Shayne Harrel (Andor Technology)</p>
</blockquote>
<blockquote>
<p>Shayne Harrel is a spectroscopy specialist for the Americas at Andor. He holds a PhD in physical chemistry from Yale University where he studied dynamical processes in charge transfer systems, magnetic materials and semiconductors using time-resolved terahertz spectroscopy. Prior to joining Andor, he was a senior applications engineer at MKS Instruments and has held previous positions as Chief Scientist-Americas for Izon Science, Senior Applications Scientist for Olympus Corporation, and as a chemist for the US Food and Drug Administration.</p>
</blockquote>
<blockquote>
<p><strong>Presenter</strong> Georgina Wynne Hughes (Editorial Team)</p>
</blockquote>
<blockquote>
<p>Georgina studied at Birmingham University where she gained a BSc in Biomedical Science, and an MSc by Research in Cancer and Genomic Sciences, which focused on defective autophagy in rare neurodegenerative diseases. As a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.</p>
</blockquote>


SelectScience: Spectroscopy techniques in practice: Methods and motivations

Spectroscopy techniques in practice: Methods and motivations

We will break down UV/VIS absorption spectroscopy, photoluminescence, and Raman scattering amongst others and examine how they work.

<p><strong>Cell line development scientists and upstream bioprocess engineers aim at optimizing biotherapeutics production processes to maximize drug product quality, yield, and manufacturing efficiency. Currently, information on critical process and product attributes required for informed decision making is often insufficient and not readily available. This has a negative impact on the process robustness, throughput, and outcome.</strong></p>
<p>Direct measurement of cell culture media and product quality attributes (PQAs) has recently been offered thanks to the Waters BioAccord™ LC-MS system with dedicated workflows for process analytics of biopharmaceuticals. The BioAccord™ LC-MS can be directly coupled to multi parallel bioreactor systems, like the Sartorius Ambr® 15 or 250 HT platforms, for accelerated process development. </p>
<p>With this integrated solution, process engineers have direct access to the information they need to be in control: drug substance quality and yield can be maximized, process and product related impurities minimized, and more robust processes can be developed, thereby de-risking batch failure. This ultimately results in accelerated drug product development and manufacturing release from months or weeks to days, saving time and resources but more importantly enable faster delivery of safer treatments to patients.  </p>
<p>Please join us as Magnus Wetterhall will present how Waters BioAccord™ LC-MS system offer enhanced capabilities for direct measurements of critical process and product quality attributes in cell line and process development and thereby enabling you to make more informed decisions with your own high-quality data. </p>
<blockquote>
<p><strong>Presenter:</strong> Magnus Wetterhall, Ph.D. (Global Marketing Manager – Bioprocessing, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Magnus Wetterhall has more than 25 years’ experience in upstream &amp; downstream bioprocessing, advanced mass spectrometry, biomarker discovery, protein characterization, working at start-ups, large biotech companies as well as academics. Magnus earned his M.Sc. and Ph.D. degree in analytical chemistry at Uppsala University, Sweden. He has published over 40 scientific papers and 50 posters in the areas of mass spectrometry, biomolecule characterization and bioseparations, and has been an invited presenter at national and international conferences on these topics. He is currently the Global Marketing Manager at Waters Corporation – Bioprocessing, where he is dedicated to deliver analytical solutions to advance upstream and downstream bioprocess.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Terence Teo (BioPharma/BMR Market Development Manager, APAC, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Terence Teo has more than 15 years of experience in bioprocessing, specializing in downstream processing R&amp;D during the early phase of his career before moving to business development roles in GE Life Sciences (now Cytiva), Pall Biotech and most recently, Waters Corporation. Terence is motivated in supporting regional customers in APAC with technologies enabling continuous bioprocessing. He is currently working actively in Waters to drive the use of LC-MS analytical solutions to support near real-time CQA measurement in bioprocess applications, as well as use of LC-MS in multi-attribute method (MAM) and emerging drug modalities such as mRNA and viral vectors. Terence earned his M.Eng degree in Chemical and Biomolecular Engineering at National University of Singapore (NUS).</p>
</blockquote>


Waters Corporation: Accelerating Upstream Bioprocess Development Through Data Driven Decisions

Accelerating Upstream Bioprocess Development Through Data Driven Decisions

We will present how Waters BioAccord™ LC-MS system offer enhanced capabilities for direct measurements of critical process and product quality attributes.

Accelerating Upstream Bioprocess Development

<p>Key Learning Points:</p>
<ul>
<li><strong>Learn about Robust Polar Pesticides analysis</strong></li>
<li><strong>Examine how to optimize a robust method for cationic and anionic pesticides</strong></li>
<li><strong>Review advances in column selectivity and MS for optimized separation of polar pesticides</strong></li>
</ul>
<p>Who Should Attend:</p>
<p>Analytical scientists interested in a new column chemistry for analysis of challenging compounds, or lab managers/ lab directors using LC-MS/MS for food and environmental analysis</p>
<blockquote>
<p><strong>Presenter:</strong> Ramkumar Dhandapani (Global Product Manager, Phenomenex)</p>
</blockquote>


Phenomenex: An Educator’s Guide to Fast and Robust Polar Pesticides Analysis

An Educator’s Guide to Fast and Robust Polar Pesticides Analysis

Learn about Robust Polar Pesticides analysis. Examine how to optimize a robust method for cationic and anionic pesticides....

Guide to Fast and Robust Polar Pesticides Analysis

<p><strong>Validate your method with a single mouse click! No need to export data into statistic programs or Excel. All steps from analysing the sample, calculating the statistical results and the reporting, everything is done in your validated Empower CDS.</strong></p>


Waters Corporation: Empower The Method Validation Manager (MVM)

Empower The Method Validation Manager (MVM)

Validate your method with a single mouse click! No need to export data into statistic programs or Excel.

<p><strong>Would you drive a car without a dashboard? And your Empower CDS Network? How much time and resources do you loose because you are not informed on time and only find out when you look under the hood?</strong></p>


Waters Corporation: waters_connect System Monitoring - the latest addition to the Empower family