The combined effect on signal response of all components of the sample, other than the analyte itself, is called the matrix effect. Sample matrix components that co-elute with the analytes of interest could cause the analytes signals to be enhanced or suppressed, compared with those measured with a standard solution of analytes (so, in the absence of matrix). This discrepancy could bring significant issues in accuracy, when not assessed and/or adjusted for.To determine matrix effect, one can do a simple experiment by comparing the extracted analyte response to the non-extracted analyte response.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Matrix_effect_formula_3b77ff748f.jpg" alt="Phenomenex: Matrix effect formula" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Matrix_effect_formula_3b77ff748f.jpg 245w," sizes="100vw" width="1175" height="134">The most frequent approach to omit undesirable matrix effects is removing the matrix through sample preparation techniques (see Figure 1). These approaches can be as simple as sample filtration, protein precipitation and liquid-liquid extraction, or more sophisticated such as solid phase extraction and immunoaffinity capture. Matrix effects can in some cases be reduced or eliminated through LC method development, by using gradients or alternative column selectivities. However, sample preparation is often preferred because it helps to extend the LC column's lifetime.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Figure_1_Common_sample_preparation_approaches_listed_in_order_of_lower_to_higher_selectivity_61e1f39c43.jpg" alt="Phenomenex: Figure 1 Common sample preparation approaches listed in order of lower to higher selectivity." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Figure_1_Common_sample_preparation_approaches_listed_in_order_of_lower_to_higher_selectivity_61e1f39c43.jpg 220w," sizes="100vw" width="428" height="304">A common example of the matrix effect is the signal suppression observed when analyzing clinical samples, such as plasma or serum, with mass spectrometry detectors. In general, these samples undergo protein precipitation as the simplest clean up approach prior to analysis, which helps reduce possible protein build up on the LC column but does not remove other contaminants. Matrix components, especially phospholipids, may have an overall negative impact on LC analyses, and could even degrade system components over time.In Figure 2: we compare the interfering signal coming from phospholipids in: (A) a serum sample that has undergone protein precipitation only and (B) a sample that has been further cleaned up using solid phase extraction with Strata™-X PRO.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Figure_2_Phospholipid_traces_obtained_from_human_serum_samples_after_protein_precipitation_7e049e93c4.jpg" alt="Phenomenex: Figure 2 Phospholipid traces obtained from human serum samples after protein precipitation." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Figure_2_Phospholipid_traces_obtained_from_human_serum_samples_after_protein_precipitation_7e049e93c4.jpg 245w," sizes="100vw" width="677" height="386">After Strata-X PRO clean up, we observed a ten-fold reduction of the interfering signal from phospholipids. This polymeric sorbent was specifically designed for enhanced matrix removal, and as demonstrated above, can remove phospholipids and harmful particulates from the sample, improving analyte recoveries as well as reproducibility.We hope this tip was useful! Check out our tool and products highlighted below.<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/strata-x-pro-solid-phase-extraction-products">Strata-X PRO SPE Products</a></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_X_PRO_SPE_Products_7d659c74c2.jpg" alt="Phenomenex: Strata-X PRO SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_X_PRO_SPE_Products_7d659c74c2.jpg 239w," sizes="100vw" width="387" height="253"><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/tools/spe-method-development">SPE Method Development Tool</a></li></ul>

Sample Preparation Tip - What is the Matrix Effect?

Laboratory and industrial users of on-site nitrogen generators often praise their convenience but also report recurring frustrations when the systems require servicing or fail unexpectedly. The most frequent complaints fall into four categories: unplanned downtime, routine maintenance burdens, costly replacement parts and service kits, and vendor discontinuations or lack of support.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Organomation_Online_Complaints_from_Nitrogen_Generator_Users_Service_Repairs_and_More_8fc094941a.jpg" alt="Organomation: Online Complaints from Nitrogen Generator Users - Service, Repairs, &amp; More" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Organomation_Online_Complaints_from_Nitrogen_Generator_Users_Service_Repairs_and_More_8fc094941a.jpg 237w," sizes="100vw" width="1080" height="712"><h4>Unplanned Downtime and Service Interruptions</h4>Several forums reveal that nitrogen generators can unexpectedly go offline, halting critical workflows until repairs are completed. In one discussion about Agilent LC–MS systems, a user explained that their nitrogen generator “would go down all the time for service,” forcing repeated mass spectrometer venting and reconditioning that consumed hours each time. Similarly, chromatographers report instrument errors—such as “drying gas flow timeout” and “nebulizer pressure timeout”—attributable to insufficient generator output, with repair suggestions ranging from tubing leaks to internal module failures, effectively causing unscheduled downtime for troubleshooting.<h4>Routine Maintenance and Part Replacement</h4>Even when generators remain online, many users find that scheduled maintenance intervals are onerous. Customers of membrane-type generators remark that service-indicator lights begin blinking after only a few thousand operating hours, prompting annual visits from field engineers. On ChromForum, one Peak Scientific owner noted that their service light blinked after each year of operation and required part replacement, whereas another operator reported that similar units ran reliably for over 2½ years without maintenance—highlighting inconsistent reliability perceptions and maintenance schedules.<h4>High Cost of Service Kits and Spare Parts</h4>In a typical analytical laboratory running an LC–MS system, the expense of maintaining an on-site nitrogen generator can quickly rival its purchase price. For instance, one ChromForum contributor noted that their annual service—including replacement of both compressor modules—was quoted at $7,500, while ordering the two compressors separately pushed the parts cost above $10,000. In another case, a mass-spectrometry lab reported receiving a third-party preventive-maintenance quote of approximately $2,400 for a single annual visit on a Peak Scientific XE35 generator, not including any replacement parts.Even routine consumables carry hefty price tags: filter kits for models such as the Peak Scientific Fusion 1010 have been listed at $1,110.09, and basic membrane filter assemblies for smaller generators often cost over $200 per kit. When these high service-kit and spare-part costs are combined with labor and travel fees, laboratories can face unexpected budget overruns—undermining the cost-saving promise of on-site nitrogen generation unless maintenance requirements are carefully planned and provisioned.<h4>Discontinued Models and Vendor Support Gaps</h4>A less frequent but acute source of frustration emerges when manufacturers discontinue models or parts. An analytical laboratory using a Parker Balston combination air/nitrogen generator discovered that the unit was no longer supported and replacement parts were unavailable, compelling the lab to revert to gas cylinders as a stopgap despite lacking a permanent cylinder hookup. Without access to spare modules or membrane replacements, users risk extended downtime or costly system overhauls.<h4>Impact on Laboratory and Industrial Operations</h4>These common complaints translate into real-world costs. Downtime for service interrupts critical analyses in pharmaceutical, food-safety and environmental labs, while on-site manufacturing processes can grind to a halt. The cumulative effect of frequent service visits, part replacements and emergency repairs drives up the total cost of ownership and, in some cases, leads users to reconsider whether on-site generation remains more economical than delivered cylinders.By recognizing these pain points—unscheduled failures, tight maintenance windows, high consumable costs and patchy vendor support—prospective buyers can better evaluate generator designs, prioritize robust after-sales service plans, and budget accurately for preventive maintenance to minimize these disruptions.<h4>Organomation as a Reliable Provider of Nitrogen Generators</h4>Based on extensive market research and customer feedback, Organomation stands out as an exemplary provider of <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=97&amp;manufacturerItems=540">nitrogen generators</a>, distinguished not only by the quality of their products but by their unwavering commitment to superior customer service. The company's remarkable 65-year legacy in the laboratory equipment industry has established us as a trusted partner for laboratories worldwide, with a proven track record that consistently delivers on both product reliability and customer satisfaction.Organomation's commitment to excellence is quantifiably demonstrated through their exceptional Net Promoter Score (NPS) of 66%, achieved in their <a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/organomation-achieves-customer-satisfaction-rating-of-excellent-in-2024-nps-survey">2024 customer satisfaction survey</a>. This score places them firmly in the "Excellent" category, significantly above the industry benchmark where scores over 50% are considered excellent.What distinguishes Organomation in today's increasingly automated business environment is their steadfast commitment to personal, human interaction. While many companies have shifted to frustrating automated systems, Organomation maintains a refreshingly direct approach: when customers call, a real person answers every time—no confusing menus, robotic voices, or endless hold times. This immediate access to knowledgeable human support allows for quick and efficient issue resolution, ensuring customers receive the assistance they need without unnecessary delays.Similarly, their live chat feature is managed by actual team members who understand their products thoroughly. This approach ensures customers receive real-time assistance from experienced professionals who can address specific technical questions and provide meaningful solutions.Organomation's nitrogen generators exemplify the company's commitment to quality and reliability. Their <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1901">NITRO-GEN+ generator</a>, featuring proprietary carbon molecular sieve technology, produces up to 99% purity nitrogen at flow rates up to 35 L/min, with integrated low-noise, oil-free compressors and on-demand 24/7 supply capability. The company's generators are designed with longevity in mind, featuring low-pressure operation that ensures longer compressor life and reduces maintenance requirements—directly addressing one of the primary concerns identified in industry forums regarding generator reliability.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Organomation_NITRO_GEN_Nitrogen_Generator_2_1438d4520c.jpg" alt="Organomation: NITRO-GEN+ generator" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Organomation_NITRO_GEN_Nitrogen_Generator_2_1438d4520c.jpg 156w," sizes="100vw" width="600" height="600"><blockquote>Looking for a more reliable partner in nitrogen generation? Organomation's team is waiting to assist. Please contact sales@organomation.com, call 978-838-7300, or start a live chat during normal business hours.</blockquote>

Online Complaints from Nitrogen Generator Users: Service, Repairs, & More

🎤 Schyler OdumIn this insightful interview, Medicinal Chemist Schyler Odum discusses his work in antibiotic drug discovery and the critical role of Ultra-High Performance Liquid Chromatography (UPLC) in his research. Schyler, a fifth-year graduate student at UTHSC, shares his expertise on:<ul><li>The importance of antibiotic drug discovery in academic institutions</li><li>How UPLC differs from HPLC and its benefits in chemical analysis</li><li>Challenges in sample preparation and potential improvements</li><li>The complexities of synthesizing large molecular weight antibiotics</li><li>The need for increased support and funding in antibiotic research</li></ul>Schyler also highlights the Gordon Research Conferences as a platform for presenting antibiotic drug discovery work. This video offers valuable insights for chemistry students, researchers, and anyone interested in the future of antibiotic development.<h3>Video Transcription</h3>Schyler Odum, a fifth-year graduate student in medicinal chemistry at UTSC, discussed his research in antibiotic discovery and his extensive hands-on experience with UPLC-MS instrumentation. With academic training in biology, biochemistry, organic chemistry, and a previous industry role at Frontier Specialty Chemicals, he now works on developing new drug candidates against Gram-positive bacterial infections and biofilms. Because large pharmaceutical companies rarely invest in antibiotic development due to low profitability, this crucial work largely depends on academic research groups like Dr. Richard Lee’s lab.A major part of Odum’s workflow relies on the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/191">Waters Acquity UPLC system</a>, which operates at ultra-high pressures to provide superior separation performance and requires only micro-scale sample quantities. As the person responsible for troubleshooting and maintaining the instrument, Odum emphasized how its sensitivity enables rapid analysis of complex synthetic intermediates. However, solvent compatibility issues between normal-phase chromatography fractions and the reverse-phase UPLC method create significant bottlenecks in sample preparation.To avoid sample precipitation and instrument damage, every fraction must be evaporated to dryness and reconstituted in appropriate solvents. This process becomes increasingly laborious when processing dozens of samples, and the lab’s outdated nitrogen blowdown equipment makes the task even more time-consuming. Despite these challenges, UPLC-MS remains essential for characterizing the unusually large antibiotic molecules Odum synthesizes—some reaching molecular weights of 1500 amu—and for validating each step of his multi-stage synthetic pathways.Odum also emphasized the urgent global need for renewed investment in antibiotic research. As resistance continues to rise, and last-resort drugs lose effectiveness, the lack of new antibiotics poses a growing threat to public health. He advocates for greater funding and awareness to ensure that critical discoveries continue to advance.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Antibiotic Drug Discovery: UPLC Analysis and Challenges in Medicinal Chemistry

Article

Product

Academy

Video

Scientists

Interview

Science and research

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

New Food magazine

Agilent Technologies

Thermo Fisher Scientific

Evosep Biosystems

Waters Corporation

<h4>HPLC Mobile Phases and Gradients</h4><ul><li>Explore the impact of mobile phase selectivity on chromatographic resolution.</li><li>Take a deep dive into typical mobile phase compositions; pH, buffers, organic solvents and other additives</li><li>Understand the significance of pH, and how to effectively design gradients</li></ul><h4>Who Should Attend</h4><ul><li>Anyone who wants to learn about basics of mobile phase selectivity in liquid chromatography (focusing on reverse phase LC) and it’s significance in method development.</li></ul><blockquote>Presenter: Namrata Saxena (Technical Manager, Phenomenex)Namrata is the Technical Manager for the Asia-Pacific region in Phenomenex and is based in Australia. As a member of the global technical team, she is responsible for assisting customers and the internal marketing and sales teams of Phenomenex for multifarious technical queries. She also support company’s bio-products portfolio through external and internal seminars/webinars, training, and technical content creation. Her competence and focus lie in providing chromatographic solutions for characterization workflows of biotherapeutics and new and emerging CGT modalities. Before joining Phenomenex she had worked as a Product Specialist and spent several years in stationary phase chemistry, column manufacturing and method development groups in a German company Bischoff Chromatography GmbH. Namrata has a post graduate degree with specialization in Analytical Chemistry.</blockquote>

Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

HPLC Mobile Phases and Gradients

Take a deep dive into typical mobile phase compositions; pH, buffers, organic solvents and other additives.

<div><h4>FFF Master Class: Master the Hardware Behind Field-Flow Fractionation</h4>Understand the inner workings and troubleshoot unexpected effects in FFF systems.A deep dive into FFF hardware, this master class is perfect for lab professionals facing challenges in field-flow fractionation.<h5>Friday, December 12: Understanding FFF hardware</h5><ul><li>Have you seen unexpected effects?</li><li>Where does the flow go?</li><li>Why do I need pressure?</li></ul><h4>Why Attend:</h4><ul><li>Deepen your understanding of FFF.</li><li>Gain actionable insights for your lab.</li><li>Interact with our expert, Dr. Andy Meyer.</li></ul><blockquote>Presenter: Dr. Andy MeyerDr. Andy Meyer joined Wyatt Technology in 2001, serving for several years as an Applications Scientist, then as Dean of Wyatt's "Light Scattering University" and as Director of Customer Service &amp; Support. After managing multiple regions in North America, Dr. Meyer is now a Senior Application Scientist for Wyatt. As a Wyatt customer, he completed his Ph.D. in Applied Science – Polymer Chemistry from the College of William and Mary in Virginia.</blockquote></div>

Waters Corporation: FFF Master Class: Master the Hardware Behind Field-Flow Fractionation

FFF Master Class: Master the Hardware Behind Field-Flow Fractionation

Join Dr. Andy Meyer on Dec 5 for a deep dive into FFF hardware. Learn to troubleshoot flow issues, pressure effects, and optimize system performance.

Master the Hardware Behind Field-Flow Fractionation

Join Waters experts as they explore EU regulations on PFAS in food, analytical challenges across complex matrices, and case studies with renowned food testing labs.baby chewing cucumber resizedPer- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants that accumulate in food and pose growing concerns for regulators, laboratories, and consumers. In response, the European Commission has introduced stringent recommendations and regulations, including (EU) 2022/1431 and (EU) 2023/915, which define limits and monitoring requirements for PFAS in food, particularly animal-origin products and infant foods.Analysing PFAS in food is scientifically demanding. Complex matrices, the diversity of PFAS structures, and strict LOQ requirements create significant analytical challenges. From fruits and vegetables to fish and baby food, laboratories face issues such as matrix effects, contamination risks, and separation of branched versus linear isomers. Eggs, in particular, represent a challenging case study due to their dense protein and fat content, yet are increasingly tested for PFAS exposure linked to environmental and dietary factors.This webinar will provide essential regulatory context, discuss critical analytical obstacles, and share practical insights into achieving accurate, robust results across diverse food types. Case studies will highlight sensitivity requirements, method performance, and analysis results, illustrating the complexities of PFAS testing and the innovations helping laboratories succeed.Attendees will gain both a broad perspective and actionable strategies to meet compliance requirements and advance food safety testing.<h4>Key learning points</h4><ul><li>Understand EU regulatory requirements and recommendations for PFAS monitoring in food.</li><li>Explore analytical challenges including matrix effects, LOQs, and isomer separation.</li><li>Learn from case studies on PFAS detection in fish, fruits, and baby food.</li><li>Gain insights into method performance, calibration, and quality assurance guidance.</li><li>Discover how environmental and dietary factors influence PFAS exposure in food.</li><li>Engage with experts and get practical answers to PFAS testing questions.</li></ul><blockquote>Presenter: Claudia Rathmann (Principal F&amp;E Market Development Manager – Waters Corporation)Experienced and passionate analytical science professional with over 20 years of technical and strategic expertise in food and environmental applications. Currently serving as Principal Market Development Manager at Waters Corporation, Claudia specialiszes in connecting customers with product management to deliver innovative solutions for complex analytical challenges. With a strong scientific foundation and a proven track record in both technical and marketing roles, Claudia bridges the gap between customer needs and product innovation across the EMEA region.</blockquote>

New Food Magazine: PFAS in Food: Regulations, Analytical Challenges, and Case Studies

PFAS in Food: Regulations, Analytical Challenges, and Case Studies

Join Waters experts as they explore EU regulations on PFAS in food, analytical challenges across complex matrices, and case studies with food testing labs.

PFAS in Food: Regulations, Analytical Challenges, Studies

Join us for an exciting seminar dedicated to the latest innovations and techniques in the biopharma industry. This event will showcase cutting-edge workflows in proteomics and structural biology, highlighting how these advancements are transforming biopharmaceutical research and development.<h4>Why Attend?</h4><ul><li>Explore customer workflows: Discover how leading companies are implementing innovative workflows to drive efficiency and effectiveness in their research and development processes.</li><li>Discover new product innovations: Learn about the latest products that are revolutionizing the biopharmaceutical industry.</li><li>Network with industry leaders: Meet with experts and peers to gain valuable insights and stay at the forefront of biopharmaceutical innovations.</li></ul><h4>Topics Covered:</h4><ul><li>Proteomics: Dive into the latest techniques and advancements in proteomics.</li><li>Structural Biology: Explore the cutting-edge developments in structural biology.</li><li>and more</li></ul><h4>Preliminary Agenda:</h4><h5>10:00 Welcome coffee registration</h5><h5>Single cell and immunopeptidomics explorations on Orbitrap-Astral&nbsp;</h5><ul><li>Farida Salimova and Jeewan Babu Rijal, LSMBO</li></ul><h5>Mastering Single Cell Proteomics: Daily Excellence with μPAC Neo Plus Trap-and-Elute Workflow&nbsp;</h5><ul><li>Jeff Op de Beeck, R&amp;D Staff Scientist, Thermo Fisher Scientific</li></ul><h5>Host Cell Protein on the Thermo Scientific Orbitrap Astral&nbsp;</h5><ul><li>Severine Clavier, Sanofi</li></ul><h5>12:00 Lunch break</h5><h5>13:30 Driving the process development of recombinant therapeutic products with automated mass spectrometry-based target peptide mapping&nbsp;</h5><ul><li>Diego Bertaccini, Merck</li></ul><h5>15:00 Coffee break &amp; networking opportunities (End of the event around 15:30)</h5><h4>Who Should Attend?</h4><ul><li>Biopharmaceutical researchers and scientists</li><li>Industry professionals and leaders</li><li>Anyone interested in the latest biopharma innovations</li></ul>Participation is free of charge. A registration is required.&nbsp; The event language is English.Don't miss out on this opportunity to enhance your knowledge and network with industry leaders. Register now to secure your space!For more information about the event or for questions regarding your registration, please contact analyze.eu@thermofisher.com.We look forward to seeing you at the seminar!<blockquote>Presenter: Information about speakers will be published soon</blockquote>

Thermo Fisher Scientific: Omics innovations for the biopharmaceutical industry

Omics innovations for the biopharmaceutical industry

Join us for an exciting seminar dedicated to the latest innovations and techniques in the biopharma industry.

Mass spectrometry (MS)-based proteomic biomarker discovery pipeline commonly begins with large-scale profiling techniques detecting thousands of candidate biomarkers. Efficiently triaging these biomarker candidates for advancement to the biomarker verification stage of the biomarker development pipeline is not trivial. Historically, immunoassays have been used as the method of choice for targeted protein quantification. However, these antibody-based methods include several limitations, including cross-reactivity due to poor specificity and selectivity, which limits the multiplexing abilities of these assays.Targeted MS methods such as parallel reaction monitoring (PRM) can overcome these limitations by identifying and quantifying peptides and proteins in complex biological samples with high accuracy, sensitivity, and reproducibility. A recent advancement in PRM is its application on a novel mass spectrometry platform, the Thermo Scientific™ Stellar™ mass spectrometer which features a dual-pressure linear ion trap mass analyzer. The Stellar MS has significantly enhanced PRM capabilities facilitating quantitative targeted MS analyses that require high measurement precision and accuracy, low limits of detection/quantification, ruggedness, and throughput. In this webinar, Dr. Thomas will provide a glimpse into how PRM on the Stellar MS can be used as a high-throughput targeted MS approach to identify and prioritize novel predictive proteomic biomarkers of drug resistance in ovarian cancer.<h5>Learning Objectives:</h5><ul><li>Learn how mass spectrometry can be applied during the verification stage of biomarker development</li><li>Understand the enhanced PRM capabilities of the Stellar MS in supporting targeted mass spectrometry applications</li><li>Learn key concepts from consensus guidelines regarding the use of targeted MS for peptide and protein quantification</li></ul><blockquote>Presenter: Stefani Thomas, PhD, University of Minnesota, Assistant ProfessorStefani earned her BA in Biological Sciences with a Minor in Music from Dartmouth College and her PhD in Pharmaceutical Sciences from the University of Southern California. After completing a research postdoctoral fellowship in the lab of Dr. Robert Cotter at Johns Hopkins, Stefani joined the Center for Biomarker Discovery and Translation at Johns Hopkins where she was a member of the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC). She then completed a Clinical Chemistry postdoctoral fellowship at Johns Hopkins and earned board certification from the National Registry of Certified Chemists and the American Board of Clinical Chemistry. Currently, Stefani is an Assistant Professor in the Department of Laboratory Medicine and Pathology at the University of Minnesota, she is the Associate Medical Director of the M Health Fairview West Bank Laboratory, and she has authored more than 70 publications. Her research laboratory applies mass spectrometry-based clinical proteomics to elucidate the biology of ovarian cancer towards improved patient stratification for therapeutic treatment.&nbsp;</blockquote>

Thermo Fisher Scientific: Breaking Barriers: Practical Approaches to Translating Proteins from Biomarker Candidates to Verified Biomarkers

Breaking Barriers: Practical Approaches to Translating Proteins from Biomarker Candidates to Verified Biomarkers

Learn how Stellar MS enhances PRM for targeted proteomics, enabling precise biomarker verification and helping identify predictive markers of drug resistance.

Approaches to Translating Proteins from Biomarker Candidates

Unlock the full potential of plasma proteomics with robust LC-MS workflows powered by Evosep. Discover how standardized, Evotip-based methods deliver exceptional depth, reproducibility, and scalability – ideal for large-cohort studies and population-level disease monitoring.<h5>BENCHMARK OF ENRICHMENT AND DEPLETION METHODS FOR QUANTITATIVE PLASMA PROTEOMICS AND THEIR CORRELATION TO CLINICAL ROUTINE MEASUREMENT.</h5><ul><li>Talk by Anders H. Kverneland, PhD., MD at Department of Oncology, Herlev and Gentofte Hospital, Copenhagen University Hospital</li></ul>In recent years several preparatory techniques for enrichment or depletion for MS proteomics have been developed to overcome the extreme dynamic range in plasma. In this study we test and benchmark several workflows with intra- and inter-sample comparisons. In addition we test the correlaton to clinical routine protein analyte measurements performed at the hospital laboratory.

Evosep Biosystems: Plasma Proteomics in translational medicine

Plasma Proteomics in translational medicine

Unlock the full potential of plasma proteomics with robust LC-MS workflows powered by Evosep. 

<div>Mycotoxins and plant toxins continue to pose a hazard in our food supply. &nbsp;With environmental factors and changing consumer preferences influencing the risk of exposure, the need for reliable, yet scalable analytical methods is paramount to enable continued monitoring.Join us for an interactive session, as we delve into LC-MS/MS based analytical methodologies developed for the determination of high impact ergot alkaloids in relevant foodstuffs to meet the needs of the food industry. Artificially contaminated samples of rye, oat, wheat, wheat gluten and baby food were extracted comparing three different solvents (acidic, neutral and alkaline) and extraction procedures with different amounts of d-SPE sorbent, to find the best combination of high extraction efficiency and effective clean-up results. Don’t miss your chance to ask our panelists your questions.<h4>Register today to explore:</h4><ul><li>Global factors and changing dynamics that may impact the risk and occurrence of contamination from mycotoxins and associated plant toxins.</li><li>Method development and analytical challenges, to meet Commission Regulations (EU) 2023/915 &nbsp;- applicable from 1st July 2024 - to ensure brand protection and consumer safety.</li><li>Considering the quantification of 8 out of 12 regulated compounds by EU (sum of –ine and –inine forms), this approach can be defined as a screening method for a reliable estimation of the risk.</li></ul><blockquote>Presenter: Eleonora ROLLO (PhD candidate – Food Safety Researcher, Barilla SpA | University of Parma)I’m a sporty girl with a deep love for good food and good wine. My curiosity has always pushed me to ask simple but essential questions: What am I eating? How is it made? Is it safe for me? These questions guided my academic path, from Food Science and Technology (Bachelor’s Degree) to Food Safety (Master’s Degree), and finally into a PhD in Barilla Group. Becoming a Food Safety Researcher was a natural choice: it allows me to combine my personal passion for food with the scientific mission of protecting consumers’ health.Scientific production documented by 1 special issues journals, 7 contributions at national and international conferences and 2 scientific papers.Presenter: Michele Suman (Food Safety &amp; Authenticity Senior Scientist Research Manager | Adjunct Professor of AgriFood Authenticity, Barilla SpA)Food Safety &amp; Authenticity Research specialist with over two decades of experience leading innovative research and development within the agri-food sector, with a track record of method development for studying contaminants, adulterants, authenticity markers and their transformation along food processing, pioneering new technologies as well. Expertise in coordinating complex, international funded projects, and a dedication to mentoring the next generation of professionals. Fostering collaboration and advancing the field through innovation with private/public stakeholders.<ul><li>Chair of the Italian National Normative Organization (UNI) Food Authenticity Working Group</li><li>Member of Joint FAO/WHO Expert Committee on Food Additives (JECFA)</li><li>Working Groups Toxins, Contaminants, Food Authenticity EU Committee for Standardization (CEN)</li><li>Member of ILSI Europe Scientific Advisory Committee &amp; Vice Chair of Food Contaminants Task Force</li><li>Coordinator of Quality-Food Safety Pillar - Italian National Cluster Agrifood Scientific Board</li><li>Co-Chair of Recent Advances in Food Analysis (RAFA) Conference series</li></ul>Scientific production documented by 6 book chapters, 3 special issues journals &amp; 3 books edited, 195 contributions at national and international conferences and 135 papers in international ISI journals.</blockquote></div>

Waters Corporation: Monitoring mycotoxins and detecting ergot alkaloids in cereals: Insights from food industry

Monitoring mycotoxins and detecting ergot alkaloids in cereals: Insights from food industry

Explore LC-MS/MS workflows for ergot alkaloid detection, extraction optimization, and meeting EU 2023/915 requirements to enhance food safety monitoring.

Monitoring mycotoxins & detecting ergot alkaloids in cereals

<div>The Waters Xevo CDMS delivers game-changing improvements in the measurement and characterization of high mass and highly heterogeneous compounds, paving the way for breakthroughs in fields such as gene therapy and structural biophysics.Join us for this webinar to explore how the Waters Xevo CDMS is revolutionizing the analysis of complex biomolecules.Learn how cutting-edge innovations are transforming research-grade technology into accessible, high-performance tools - enabling simplified, compliant workflows and deeper insights into rAAVs, VLPs, mRNA/LNPs, and more.<ul><li>Gain insights on Waters Xevo CDMS product, from the nanoESI emitter, to ELIT. Learn the development journey of the research grade CDMS technology from Indiana University into a commercial product that enables the democratization of the novel technology.</li><li>Learn application space of the Waters Xevo CDMS, hearing application examples based on collaboration with Key Opinion Leaders from the Pharma and Biotech industry and from various research institutes.</li><li>Hear from Pfizer scientist to examine how ELIT CDMS has evolved through commercialization, address key considerations for introducing Xevo CDMS into an industrial setting and highlight user experiences across multiple modalities with Xevo CDMS.</li></ul><blockquote>Presenter: Lauren Barnes, Ph.D. (Senior Scientist, Pfizer)Dr. Lauren Barnes is a Senior Scientist at Pfizer within the Biotherapeutics and Pharmaceutical Sciences division at Pfizer. She joined Pfizer September 2022 as a mass spectrometrist within the Analytical Research &amp; Development (ARD) organization performing heightened characterization on biotherapeutic molecules, including proteins and gene therapies. In this role, she has expanded her expertise to include mass photometry, leveraging her background in the analysis of large, intact biomolecular species to develop methods for species characterization with this emerging technique. Lauren has contributed to collaborative efforts involving CDMS, both during her graduate research and in her professional role at Pfizer.Lauren received her Ph.D. in Analytical Chemistry from Indiana University Bloomington in 2022. Her graduate research focused on the analysis of intact viral gene therapy systems using charge detection mass spectrometry (CDMS) in the lab of Dr. Martin Jarrold.Presenter: Jakub Ujma, Ph.D. (Principal Consulting Technical Product Lead, Waters Corporation)Jakub works at Waters Corporation as a Principal Consulting Technical Product Lead in mass spectrometry R&amp;D. His work has focused on Charge Detection Mass Spectrometry (CDMS), with applications in the analysis of large biomolecules such as protein complexes and virus particles. Jakub leads the development of the Waters Xevo™ CDMS system, which utilizes an electrostatic linear ion trap to enable unambiguous mass measurement of megadalton-sized molecules. In addition to CDMS, Jakub also contributed to a number of projects involving ion mobility and time-of-flight (ToF) mass spectrometry, such as hardware design and performance characterization. He has co-authored several research papers and regularly participates in scientific meetings and discussions within the mass spectrometry community.Jakub received his PhD in Chemistry from Manchester University with a focus on ion mobility techniques for studying protein structure.Presenter: Anisha Haris, Ph.D. (Senior Applications Scientist, Waters Corporation)Anisha is a Senior Applications Scientist in the Charge Detection Mass Spectrometry (CDMS) Program at Waters Corporation. Her work focuses on supporting the evaluation, development, and commercialization of charge detection mass spectrometry (CDMS) for the precise characterization of large, complex, and heterogeneous biomolecules - including protein complexes, virus-like particles, and viral vectors. By bridging cutting-edge instrumentation with real-world applications, Anisha supports the expansion of CDMS through close collaboration with researchers and industry partners in biopharmaceutical development, structural biology, and virology.Anisha received her Ph.D. from the University of Warwick. Her dissertation was focused on “Advanced Applications of Mass Spectrometry for Isomer Differentiation and Analysis of Biomolecules”.</blockquote></div>

Waters Corporation: Unveiling the Waters Xevo CDMS: Biomolecule Characterization Redefined

Unveiling the Waters Xevo CDMS: Biomolecule Characterization Redefined

Join us for this webinar to explore how the Waters Xevo CDMS is revolutionizing the analysis of complex biomolecules.

Waters Xevo CDMS Biomolecule Characterization

As demand for high lab productivity continues to increase, it is essential to ensure your LCMS system is ready to perform every day. In this webinar, we will discuss best practices and preventative maintenance tips to help maximize your instrument’s uptime. We will also take a quick look at column choices to help you get the most from your Agilent LCMS system.<blockquote>Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.</blockquote>

Agilent Technologies: Getting the Most from Your Agilent LCMS System

Getting the Most from Your Agilent LCMS System

In this webinar, we will discuss best practices and preventative maintenance tips to help maximize your instrument’s uptime.

Join us as we celebrate The Agilent Infinity III LC Series turning one. Welcome to the Agilent Technologies Infinity III LC Series Webinar - The Infinity III Transition - Easing your usual regulatory pain points.<h4>Key learning objectives:</h4><ul><li>Learn what's new from Agilent Infinity III LC systems with it's SMART features.&nbsp;</li><li>Understand the ease of upgrading your LC systems within a regulatory laboratory and when connecting to your current CDS system.&nbsp;</li><li>Allow us to share our special promotion to update your Infinity II systems – marking the one-year anniversary of the Agilent Infinity III launch.</li></ul><h4>Who should attend?</h4>Laboratory managers, lab instrument purchasing decision makers, lab analysts and end users of chromatography systems, and quality departments. &nbsp;<blockquote>Presenter: Shaun Pritchard (Product Specialist Liquid Phase Separations, Agilent Technologies)Shaun Pritchard is a Product Specialist for separation products at Agilent Technologies covering the UK and Ireland. He started his career in 1989 working at a large Pharmaceutical company holding a number of roles, this is where he became passionate about HPLC finally in 1998 he took the leap to leave the lab and move to the commercial side of things and has had a number of roles within the industry until 2017 he joined Agilent.</blockquote>

Agilent Technologies: Seamless Compliance – Effortless Transition from Infinity II to Infinity III LC Series