Understanding the fundamentals of the on-off retention mechanism of oligonucleotides and their application to high throughput analysis

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 13th January 2025? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Thermo Fisher Scientific,</strong> <strong>Shimadzu</strong>,<strong> </strong>a presentation by <strong>Agilent Technologies</strong>, a manual by <strong>Metrohm,</strong> and a poster from <strong>ASMS </strong>by <strong>Waters Corporation</strong>!</p><h4><strong>1. Thermo Fisher Scientific: High-resolution DIA proteomics workflow for single-cell samples on the Orbitrap Astral mass spectrometer</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28638"><strong>Full PDF for download</strong></a></li></ul><p>Assess proteome coverage and sample throughput performance for single-cell samples using a library-free and library-based data-independent acquisition (DIA) method on the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer.</p><h5><strong>Introduction</strong></h5><p>In recent years, there has been a shift to analyzing smaller sample quantities, driven by the interest in profiling the proteome of individual cells. This increased focus on LC-MS analysis of limited sample amounts, e.g., single cells, requires the highest possible sensitivity. At the same time, throughput and chromatographic performance must be preserved to generate sufficiently high-quality data from large data sets to draw meaningful conclusions. This trend toward analyzing smaller sample quantities reflects the need to understand the heterogeneous nature of biology through the dissection of complex systems into their individual parts or, specifically, individual cells. Traditional proteomics approaches read out the average of a sample while single-cell proteomics provides insights into the exact nature of each of the components in such samples.</p><p>The Orbitrap Astral mass spectrometer delivers a new standard for single-cell samples with deeper proteome coverage and faster throughput. It enables these capabilities through the synchronized acquisition of high-resolution and high-dynamic range full scan spectra with the Thermo Scientific™ Orbitrap™ mass analyzer and high speed and sensitivity MS/MS spectra with the Thermo Scientific™ Astral™ mass analyzer. The Thermo Scientific™ Vanquish™ Neo UHPLC system delivers maximum performance in terms of precise, low flow rates and gradient formation, as well as low injection volumes—an important benefit for single-cell proteomics and limited sample amounts.</p><p>The Thermo Scientific™ μPAC™ Neo HPLC columns with novel micro-pillar array deliver highly reproducible chromatographic separation and increased sensitivity with maximum resolution.<br>The Thermo Scientific™ FAIMS Pro Duo interface enhances sensitivity by removing background signals.</p><p>In this technical note, we present a single-cell proteomics workflow (Figure 1). To first evaluate sensitivity and quantitative precision and accuracy and linearity of the workflow, a dilution series from 5 ng to 50 pg of the Thermo Scientific™ Pierce™ HeLa protein digest standard was analyzed.</p><h4><strong>2. Shimadzu: Automatic Optimization of Gradient Conditions by AI Algorithm - Consecutive Optimization at Different Column Oven Temperatures</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28642"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>The AI algorithm of LabSolutions MD can automatically optimize gradient conditions to greatly reduce labor of LC method development.</li><li>Gradient conditions are automatically optimized at different column oven temperatures consecutively.</li><li>Automatic optimization of gradient conditions can be applied not only to new method development, but also to existing method to efficiently improve resolution.</li></ul><h5><strong>Introduction</strong></h5><p>In the typical LC method development, the process begins with “preparation” which includes mobile phase preparation, column installation, and creation of analysis schedules, then the analysis is started. After that, the acquired data is analyzed and “preparation” for the subsequent analysis is carried out, followed by starting the next analysis again. The method development progresses by repeating these processes, but in addition to the significant time required to repeatedly create analysis schedules, expertise in chromatography is necessary to explore optimal conditions based on data analysis. In other words, typical method development requires “human intervention”. Therefore, eliminating human involvement and automating such method development processes would be desirable to improve labor efficiency. This article introduces an approach for automatically optimizing gradient conditions at different column oven temperatures consecutively, identifying the combination of temperature and gradient conditions that meet the resolution criteria.</p><h5><strong>Conclusion</strong></h5><p>Automatic optimization of gradient conditions using AI algorithm of LabSolutions MD was applied to a model sample (mixture of seven compounds of small molecule) at different column oven temperatures. As a result, the temperature and gradient conditions that met the resolution criteria were successfully explored. In method development, human intervention, such as analysis batch creation and data analysis, is required to optimize gradient conditions. LabSolutions MD can provide significant labor savings in this area. For more information on LabSolutions MD, please refer to the Technical Report <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/c190_e284_1ca86b48ea/c190-e284.pdf">“Efficient Method Development Based on Analytical Quality by Design with LabSolutions MD Software (C190-E284)”</a>.</p><h4><strong>3. Agilent Technologies: Optimize Your Polymer Analysis by Selecting the Ideal GPC/SEC Column</strong></h4><ul><li>Presentation</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28578"><strong>Full PDF for download</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4481"><strong>Recorded webinar</strong></a></li></ul><p>&nbsp;</p><ul><li><strong>GPC/SEC Separation Mechanism</strong></li><li><strong>Conventional GPC/SEC Workflow</strong></li><li><strong>Considerations for GPC/SEC Column Selection (Key questions to ask)</strong><ul><li>What polymer are you analysing?</li><li>Which solvent (or solvents) is your polymer soluble in?</li><li>What is the expected molecular weight range of your polymer?</li><li>What is the requirement for your analysis or what would you like to improve about your existing GPC/SEC separation?<ul><li>Resolution is important</li><li>Reproducibility of sample chromatography and results</li><li>Speed of analysis or sample throughput is something to improve on</li></ul></li></ul></li><li><strong>Further considerations</strong><ul><li>Know the properties of the sample</li><li>Be familiar with the properties of the columns being considered</li><li>It is important to balance polarities for the sample, solvent, and column packing</li></ul></li><li><strong>Common Column Chemistries for GPC/SEC</strong></li><li><strong>Agilent Magic Triangles</strong></li><li><strong>Column Selection: Porosity</strong></li><li><strong>Individual Pore Versus MultiPorous Particle</strong></li><li><strong>Column Selection: Particle size</strong></li><li><strong>Columns for Low Polarity Organic Eluents</strong></li><li><strong>Columns for Medium Polarity Organic Eluents</strong></li><li><strong>Columns for High Temperature GPC/SEC</strong></li><li><strong>Columns for Fluorinated Organic Eluents</strong></li><li><strong>Columns for Aqueous Eluents: Neutral or weak Anions</strong></li><li><strong>Columns for Aqueous Eluents: Polycations and Strong Polyanions</strong></li><li><strong>Column Selection: Additional Considerations</strong></li><li><strong>Columns in Series</strong></li><li><strong>Wrongly Coupled Columns</strong></li><li><strong>Combination of Individual Pore Size Columns</strong></li><li><strong>Column Selection and Importance of Solvent Choice</strong></li><li><strong>Solvent Selection</strong></li><li><strong>Successful Solvent Choice</strong></li><li><strong>Eluent Modification in Organic GPC</strong></li><li><strong>Common Questions Around GPC/SEC Standards</strong></li><li><strong>Agilent Polymer Calibration Standard Offerings</strong></li><li><strong>Well Characterized Polymer Standards</strong></li><li><strong>Easivial Certificate of Analysis</strong></li><li><strong>Common Questions Around SEC Standards</strong></li><li><strong>Installation and Care of Your Column</strong></li><li><strong>Before Your Analysis</strong></li><li><strong>Column Selection – Additional considerations</strong></li><li><strong>System Tubing</strong></li><li><strong>Your GPC/SEC Toolbox</strong></li></ul><h4><strong>4. Metrohm: Installation Instruction for ProfIC Vario 7 Cation</strong></h4><ul><li>Manual</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28584"><strong>Full PDF for download</strong></a></li></ul><p>The ProfIC Vario 7 Cation is a Professional IC Vario system with Inline Dilution and Inline Dialysis for the fully automatic determination of cations in high concentrations in matrices containing emulsions. This system enables users to dilute the sample and eliminate droplets of fat or organic solvents or particles prior to the analysis.</p><h4><strong>5. Waters Corporation: IMPROVED TISSUE HOMOGENIZATION AND SPE-BASED SAMPLE PREPARATIONS FOR THE QUANTITATIVE LC-MS ANALYSIS OF OLIGONUCLEOTIDE THERAPEUTICS</strong></h4><ul><li>Poster/ASMS</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/27580"><strong>Full PDF for download</strong></a></li></ul><p>Efficient extraction of drug analytes is a critical aspect of drug metabolism and pharmacokinetics (DMPK) studies. This has long applied to small molecules and still applies to the bioanalysis of oligonucleotides. Oligonucleotide drugs and their metabolites must be quantified within both biofluids and tissue samples. The newest of oligonucleotide drugs are both extensively modified and conjugated. These modified residues and conjugate moieties can complicate extraction recovery and reproducibility. In this work, we report several key insights on how to achieve improved extractions. Protocols for solvent-assisted proteinase K sample pre-treatments are investigated using a weak anion exchange (WAX) microplate-based solid phase extraction (SPE) device. Direct injection LC-MS quantitation is demonstrated for all three antisense oligonucleotides (ASOs).</p><h5><strong>CONCLUSIONS</strong></h5><ul><li>Selective extraction and elution of ASO oligonucleotides is feasible from multiple tissue types.</li><li>Extracted ASOs are amenable for either full scan mode analysis on benchtop small footprint TOF MS or MRM analysis by Triple Quad MS</li><li>Reproducible and repeatable data were collected even without the application of an internal standard</li><li>One novel aspect of the SPE eluate is that no further sample preparation is required for subsequent LC-MS analysis saving time and avoiding sample losses during drying and reconstitution</li><li>Eluted nucleic acid components can be readily analyzed by regular ion-pairing reversed phase chromatography and UV/MS-based detection and quantitation</li><li>This robust tissue extraction protocols for improved LC-MS based bioanalysis of ASO drugs was repeatable and highly reproducible across multiple batches of SPE sorbent, providing confidence in the suitability of the optimized protocols</li></ul>

News from LabRulezLCMS Library - Week 3, 2025

<p><strong>In the week of January 20, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over&nbsp;</p><p><strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Characterization and sequence mapping of large RNA and mRNA therapeutics using HRAM</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4580">Registration</a></div></li></ul></div><p>The presentation showcases the innovative partial T1 digest strategy developed at the University of Sheffield for sequencing mRNA therapeutics.</p></div><h5><strong>2.&nbsp;SelectScience/Agilent Technologies: From Start to Finish: How Agilent Can Support Better Decisions in IVT mRNA-Based Biopharmaceutical Analysis</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4674">Registration</a></div></li></ul></div><p>We will discuss how automated electrophoresis and LC/MS systems solutions from Agilent provide reliable insights for IVT mRNA workflows at many steps.</p></div><h5><strong>3.&nbsp;Phenomenex: LC Troubleshooting Quick Guide: Spot Problems Fast &amp; Fix Them Like a Pro!</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4676">Registration</a></div></li></ul></div><p>In this webinar - General troubleshooting approach, HPLC troubleshooting steps and diagnosing tips and preventive maintenance and column care.</p></div><h5><strong>4.&nbsp;Thermo Fisher Scientific: State-of-the-art E&amp;L is not rocket science</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4581">Registration</a></div></li></ul></div><p>Ulli Honester, a team leader from Solvias, presented on advancements in mass spectrometry for identifying extractables and leachables (E&amp;L).</p></div><h5><strong>5.&nbsp;Waters Corporation: Empower Tips: Ask Me Anything About Navigating Data Governance - Risk and Vigilant Monitoring</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4550">Registration</a></div></li></ul></div><p>We will explore critical aspects of data governance and critical thinking, insights and strategies to help navigate the current environment, evolving your approach and modernizing your way of working.</p></div><h5><strong>6.&nbsp;Evosep Biosystems: A beginner’s guide to Evosep 2025</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4548">Registration</a></div></li></ul></div><p>This webinar is your opportunity to gain a comprehensive understanding of the Evosep One’s key features and how it can seamlessly integrate into your current processes.</p></div><h5><strong>7.&nbsp;Shimadzu Corporation: ASTM D8421 and D8535: Cost Effective Methods for Rapid PFAS Testing in Wastewater and Soil</strong></h5><div><div><ul><li><div>Th, 23.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4465">Registration</a></div></li></ul></div><p>Discover simplified standardized methods for PFAS analysis based on co-solvation as sample preparation.</p></div>

Webinars LabRulezLCMS Week 4/2025

Article

Webinars

Application

Scientific article

Various

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Merck

Mettler-Toledo

Agilent Technologies

US HUPO

GERSTEL

Shimadzu Corporation

<p><strong>Learn how to test nonvolatile and semi-volatile PFAS compounds in consumer products by LC-MS/MS as outlined in a method being standardized by ASTM.</strong></p><p>We will host a Q&amp;A session after the presentation to address any questions or comments.</p><blockquote><p><strong>Presenter:</strong> &nbsp;Om Shrestha (Senior Research Scientist, Shimadzu Scientific Instruments)</p><p>Om Shrestha is a highly skilled and results-driven Senior Research Scientist with extensive expertise in analytical method development, optimization, and analysis. With a proven track record in delivering exceptional technical support, Om is dedicated to advancing cutting-edge analytical techniques and ensuring compliance with industry standards. In his current role as Senior Application Scientist at Shimadzu Scientific Instruments, Om specializes in PFAS analysis and method development using Shimadzu LCMS, with a comprehensive understanding of methods to support regulatory frameworks such as EPA 533, 537, and 1633. He excels in leading complex projects, troubleshooting instrumentation, and collaborating with cross-functional teams to meet customer and market demands.<br><br><strong>Presenter:</strong> Ruth Marfil-Vega, PhD (Sr Market Manager – Environmental, Shimadzu Scientific Instruments)</p><p>Dr. Ruth Marfil-Vega has 20 years of expertise in environmental chemistry and engineering in the US and Europe. She has worked with the EPA and the utilities industry to design and execute comprehensive solutions for addressing new environmental and water quality issues, including PFAS, regulated and unregulated DBPs, and other emerging contaminants. Since Ruth joined Shimadzu five years ago, she has continued to work with environmental stakeholders to ensure that analytical instrumentation and workflows support their success. She earned a BSc in Chemistry from the University of Valladolid (Spain) and a PhD in Environmental Science and Engineering from the University of Cincinnati.</p></blockquote><h3><strong>PFAS in Focus</strong></h3><p><strong>Welcome to PFAS in Focus, a comprehensive and exclusive webinar series sponsored by Shimadzu, covering the basics and beyond about the analysis of Per- and Polyfluoroalkyl Substances (PFAS) in the environment.</strong></p><p>Join our experts, live or on-demand after the original air date, to demystify the challenges of PFAS analysis and gain the insights you need to:</p><ul><li>Manage PFAS contamination in your laboratory.</li><li>Master standardized methods for PFAS testing.</li><li>Discover new analytical workflows to maximize the performance of your LC-MS/MS for PFAS analysis.</li></ul><p>Who should attend:</p><ul><li>Environmental industry professionals and academics currently working on PFAS analysis or looking to establish the application in their labs.</li><li>Representatives from drinking and wastewater utilities.</li><li>LC–MS users from any field interested in expanding their applications to include PFAS testing.</li></ul>

Analyzing Consumer Products for PFAS: New Method Development

Learn how to test nonvolatile and semi-volatile PFAS compounds in consumer products by LC-MS/MS as outlined in a method being standardized by ASTM.

<p><strong>Size exclusion chromatography (SEC) is a noninteractive technique that separates solutes according to their molecular size in non-denaturing conditions. It is often used as the first step in isolation of a protein from a crude sample. SEC is a gold standard to determine aggregate and fragment impurities in biotherapeutics.</strong></p><p>In this webinar, we will outline basic theory, principles, and practical guidelines on column selection for SEC method development. We will overview biotherapeutic aggregation and how method parameters such as flow rate, column length, injection volume, and pH and salt concentration influence SEC.</p><blockquote><p><strong>Presenter:</strong> Mohit Patel (Application Engineer, Agilent Technologies, Inc.)</p><p>Mohit Patel is part of the CSD Online AE Team. His primary focus is to support SMLC, Bio-Columns, and consumables.</p><p>Before joining Agilent, Mohit was an Assistant Professor of Chemistry at Temple University. Mohit developed his passion for science through his experiences as a faculty researcher and a mentor. Mohit actively taught, developed curriculum, and served as senior personnel, with a leadership position, on funded grants, mentored research projects, and co-coordinator of STEAM related outreach services.</p><p>Mohit has earned a Doctorate degree in Chemistry from Temple University in 2016.</p></blockquote>

Agilent Technologies: Becoming a Better Chromatographer - Educational Webinar Series

Size Exclusion Chromatography of Biomolecules: Column Selection and Method Optimization

We will outline basic theory, principles, and practical guidelines on column selection for SEC method development.

SEC of Biomolecules: Column Selection and Methods

<p><strong>In a typical laboratory, quality control mishaps are inevitable during sample preparation. For example, a sample going missing or a sample being miss-identified will result in downstream effects including the loss of significant time, money and samples. Therefore, advances in digitalization have been shown to reduce human error as manual input and transcription is eliminated from the process. Consequently, this lowers the likelihood of obstacles such as these, to improve data accuracy and process efficiency.</strong></p><p>Join Dr. Kelsey Mato, Product Marketing at Agilent Technologies, to hear about a new technology, linking samples and software for unprecedented control, to give you confidence in your laboratory workflow.</p><p><strong>Key learning objectives:</strong></p><ul><li>Understand the long-term risks and costs of manual sample tracking</li><li>Learn how taking steps toward digitalization can reduce errors in sample management</li><li>Find a new solution for seamlessly linking samples and software</li></ul><p><strong>Who should attend?</strong></p><p>Lab managers, and experienced and inexperienced lab technicians.</p><p><strong>Certificate of attendance</strong></p><p>All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</p><blockquote><p><strong>Presenter: </strong>Dr. Kelsey Mato (Product Marketing, Agilent Technologies)</p><p>Dr. Kelsey Mato completed her PhD in analytical chemistry at Louisiana State University. Her career began focusing on sensing devices in the biopharmaceutical industry as well as her involvement in software for a wide range of instruments. Ensuing this propelled her efforts to specialize in analytical instrumentation where currently she is working as a product marketing professional at Agilent Technologies.</p><p><strong>Presenter:</strong> Olivia Long (Editorial Team, SelectScience)</p></blockquote>

Agilent Technologies: Eliminate errors with automation and stress-free sample tracking

Eliminate errors with automation and stress-free sample tracking

Join us to hear about a new technology, linking samples and software for unprecedented control, to give you confidence in your laboratory workflow.

Eliminate errors with automation and easy sample tracking

<p><strong>Experience the LC-GC-FID analysis for MOSH/MOAH in our informative webinar! We will introduce you to the key features of the GERSTEL LC-GC-FID system and show you how it simplifies your daily work.</strong></p><p><strong>Highlights of the webinar:</strong></p><ul><li>Introduction to LC-GC-FID analysis technology for MOSH/MOAH determination</li><li>GERSTEL Early Vapor Exit for the efficient removal of large solvent amounts</li><li>Advantages of the unique GC column arrangement</li></ul><p>Save your place in the webinar now and bring your MOSH/MOAH analysis to a new level!</p><blockquote><p><strong>Presenter: </strong>Isabelle Heker</p><p><strong>Presenter: </strong>Timo Köhler, Ph.D.</p></blockquote>

GERSTEL:  Determination of mineral oil residues HPLC-GC analysis of MOSH & MOAH

Determination of mineral oil residues HPLC-GC analysis of MOSH & MOAH

Experience the LC-GC-FID analysis for MOSH/MOAH in our informative webinar! We will introduce you to the key features of the GERSTEL LC-GC-FID system

Determination of mineral oil residues HPLC-GC analysis

<p><strong>Enter the world of automated sample preparation for MOSH/MOAH analysis! In this webinar we will show you the different sample preparation methods for MOSH &amp; MOAH analyses and their automation with the GERSTEL MultiPurpose Sampler. Our experts will explain the necessity of sample preparation as well as the methods - saponification, epoxidation and AlOx purification - required for the different matrices.</strong></p><p><strong>Highlights of the webinar:</strong></p><ul><li>Necessity and benefits of sample preparation for MOSH/MOAH analysis</li><li>Detailed insights into workflows for automated sample preparation</li><li>Expert knowledge on selecting the suitable methods for different matrices</li></ul><p>Register now and automate your MOSH/MOAH sample preparation!</p><blockquote><p><strong>Presenter: </strong>Isabelle Heker</p><p><strong>Presenter: </strong>Timo Köhler, Ph.D.</p></blockquote>

GERSTEL: Efficiency boost through automation MOSH/MOAH

Efficiency boost through automation MOSH/MOAH

In this webinar we will show you the different sample preparation methods for MOSH & MOAH analyses and their automation with the GERSTEL MultiPurpose Sampler.

<h4><strong>Eight Webinars and a Roundtable with Experts from Merck&nbsp;</strong></h4><p><strong>Analytical Excellence Week' is organized to empower professionals in the field of analytical chemistry. Over the course of eight insightful sessions, industry experts will share their knowledge and best practices to streamline your analytical workflows and enhance your efficiency.</strong></p><p>From <strong>understanding quality grades</strong> in inorganics and solvents to <strong>exploring sustainable practices</strong> in the lab, each webinar covers essential topics that will enhance your analytical capabilities. Learn how to <strong>optimize your Karl Fischer titration</strong>, achieve precision in PFAS testing, and gain <strong>troubleshooting tips for LC-MS</strong> to effectively address common challenges and improve your laboratory results.<br>The sessions will also address critical aspects of <strong>safety and sustainability</strong>, ensuring you are well-equipped to handle hazardous chemicals responsibly while making strides toward greener laboratory practices.</p><p>This is an invaluable opportunity to gain insights that can significantly optimize your analytical processes. Don’t miss your chance to elevate your expertise and contribute to a more efficient and sustainable future in analytical workflows.<br>Each session is specifically designed to address essential topics that directly impact your daily practices, making it relevant for all professionals working in a chemical analysis laboratory.</p><h3><strong>Day 2: Tuesday, 11 February</strong></h3><h4><strong>Dihydropinene, γ-Valerolactone, and Dimethyl Isosorbide: Bio-Sourced Versatile Green Solvents for Diverse Organic Synthesis</strong></h4><p><strong>The growing environmental concerns and regulatory restrictions on conventional organic solvents derived from non-renewable sources have spurred the search for greener, sustainable alternatives. This presentation will explore the potential of dihydropinene (DHP), γ-valerolactone (GVL), and dimethyl isosorbide (DMI) as bio-sourced and biodegradable solvents, offering a promising solution to replace toxic and non-renewable solvents. DHP, derived from turpentine oil, is an effective substitute for solvents such as n-hexane, benzene, and dichloromethane, while GVL and DMI are ideal replacements for hazardous solvents such as DMF, NMP, and DMA.</strong></p><p>These solvents not only perform well in various chemical transformations but also offer significant environmental benefits due to their biodegradability, low toxicity, and recyclability. The presentation will highlight the applications of DHP, GVL, and DMI in green chemistry, showcasing their potential in organic synthesis and industrial processes. It will also discuss their role in advancing sustainable practices, reducing dependency on fossil-based solvents, and promoting safer working environments. By focusing on green solvent solutions, the presentation will emphasize their future potential in scientific research, production, and industrial applications.</p><p><strong>By attending this webinar, you will learn:</strong></p><ul><li><strong>Sustainable Solvent Alternatives:</strong> How bio-based solvents such as DHP, GVL, and DMI can replace conventional toxic solvents, providing eco-friendly, biodegradable options for chemical processes.</li><li><strong>Environmental and Safety Benefits:</strong> The environmental advantages of these solvents, including biodegradability, low toxicity, and recyclability, and how they promote safer and greener practices in research and industry.</li><li><strong>Practical Applications:</strong> How these solvents can be effectively used in organic synthesis, industrial processes, and various scientific fields, contributing to more sustainable and efficient chemical solutions.</li></ul><p><strong>Who should attend:</strong><br>This presentation will benefit those working in pharmaceutical and drug discovery R&amp;D, process development of API, environmental scientists and sustainability experts, industry professionals in chemical engineering and manufacturing, materials science researchers, and students and researchers in green chemistry.</p><blockquote><p><strong>Presenter:</strong> <i>Saikat Sinha, (R&amp;D Manager, Merck)&nbsp;</i></p></blockquote><h4><strong>Enhance Your PFAS Testing: Achieve Precision and Reduce Background Noise with Specialized Consumables</strong></h4><p><strong>Join this presentation to explore advanced LC-MS/MS methods for determining PFAS analytes at low levels across diverse samples and matrices. Learn how to identify contamination sources, reduce background noise, and improve testing precision with specific recommendations on PFAS-qualified consumables. Discover various LC-MS/MS PFAS testing workflows and strategies to minimize errors, supported by data from both regulated and non-regulated methods.</strong></p><p><strong>By attending this webinar, you will learn:</strong></p><ul><li>Detailed insights into different LC-MS/MS PFAS methods</li><li>Techniques to identify and test contamination sources</li><li>Strategies to reduce background noise</li><li>Practical tips and tricks for improved testing</li></ul><p><strong>Who should attend</strong>:<br>This webinar is essential for lab technicians, lab managers, and quality assurance managers involved in developing in-house methods or verifying regulated testing protocols such as EPA Methods 533, 537.1, and 1633.</p><blockquote><p><strong>Presenter: </strong>Patrik Appelblad, (Fellow and Technical Lead, Analytical Chemistry R&amp;D, Merck)&nbsp;</p></blockquote>

Merck: Webinar Series & Expert Roundtable

Webinar Series & Expert Roundtable (Day 2)

(Day 2) - Part 1: Dihydropinene, γ-Valerolactone, and Dimethyl Isosorbide: Solvents for Organic Synthesis and Part 2: Enhance Your PFAS Testing

<h4><strong>Eight Webinars and a Roundtable with Experts from Merck</strong></h4><p><strong>Analytical Excellence Week' is organized to empower professionals in the field of analytical chemistry. Over the course of eight insightful sessions, industry experts will share their knowledge and best practices to streamline your analytical workflows and enhance your efficiency.</strong></p><p>From <strong>understanding quality grades</strong> in inorganics and solvents to <strong>exploring sustainable practices</strong> in the lab, each webinar covers essential topics that will enhance your analytical capabilities. Learn how to <strong>optimize your Karl Fischer titration</strong>, achieve precision in PFAS testing, and gain <strong>troubleshooting tips for LC-MS</strong> to effectively address common challenges and improve your laboratory results.</p><p>The sessions will also address critical aspects of <strong>safety and sustainability</strong>, ensuring you are well-equipped to handle hazardous chemicals responsibly while making strides toward greener laboratory practices.</p><p>This is an invaluable opportunity to gain insights that can significantly optimize your analytical processes. Don’t miss your chance to elevate your expertise and contribute to a more efficient and sustainable future in analytical workflows.</p><p>Each session is specifically designed to address essential topics that directly impact your daily practices, making it relevant for all professionals working in a chemical analysis laboratory.</p><h3><strong>Day 1: Monday, 10 February</strong></h3><h4><strong>Navigating Quality Grades Part 1: Inorganics and Solvents for Classical Analysis and Research</strong></h4><p><strong>Join us for an insightful webinar focused on the critical process of selecting the right quality grade for inorganic reagents and solvents used both in analytical and research applications. We will provide an in-depth overview of various quality grades, including ACS grade and EMSURE®, and discuss their specific attributes, including purity, regulatory support, and packaging. Gain valuable insights into the importance of this choice and its impact on the quality of your lab processes and outcomes.</strong></p><p><strong>By attending this webinar, you will:</strong></p><ul><li>Understand the significance of choosing the right quality grade for your lab applications</li><li>Explore the available quality grades, including ACS grade and EMSURE®, and their specific attributes</li><li>Recognize the impact of quality grade selection</li><li>Learn best practices for selecting and utilizing the appropriate quality grade for several applications</li></ul><p><strong>Who should attend:</strong></p><p>This webinar is designed for professionals involved in laboratory work who seek to enhance their understanding of quality grades for inorganic reagents and solvents. It is particularly relevant for lab technicians, researchers, and managers in analytical labs (like QC, environmental, etc.) method development and research laboratories that are looking to optimize their processes and outcomes.</p><blockquote><p><strong>Presenter:</strong> Christian Roth (Senior Product Manager Inorganics, Merck)</p><p><strong>Presenter:</strong> Lyra Christianson (Product Manager Solvents, Milwaukee, Wisconsin, USA, an affiliate of Merck)</p></blockquote><h4><strong>Navigating Quality Grades Part 2: Solvents and Reagents for Chromatography Applications</strong></h4><p><strong>This second part focuses on the critical process of selecting the right quality grades used in chromatography applications. We provide an in-depth overview of various quality grades, from Suprasolv® solvents for GC to LiChrosolv® solvents for HPLC, in combination with LiChropurTM reagents, and discuss their specific attributes, including purity, regulatory support, and packaging. The topic will be illustrated by typical HPLC and LC-MS applications.</strong></p><p><strong>By attending this webinar, you will learn:</strong></p><ul><li>Which grades are suitable for which chromatographic analysis</li><li>What are the specific attributes of the solvents and reagents</li><li>How to combine different quality grades meaningfully</li><li>How to identify and differentiate between various quality grades of reagents and solvents.</li><li>To understand the critical importance of selecting the appropriate quality grade for your specific analytical applications</li></ul><p><strong>Who should attend</strong></p><p>Lab technicians, lab managers, and quality control-related persons who need to understand how to select the different quality grades for HPLC and LC-MS applications.</p><blockquote><p><strong>Presenter: </strong>Patric Klein, (Global Product Manager Organic and Inorganics Instrumental Analysis, Merck)</p><p><strong>Presenter: </strong>Fernando Luengo, (Global Product Manager HPLC Solvents, Milwaukee, Wisconsin, USA, an affiliate of Merck)</p><p><strong>Presenter: </strong>Daniel Weibel, (Global Product Manager Solvents, Merck)</p></blockquote>

Webinar Series & Expert Roundtable (Day 1)

Navigating Quality Grades Part 1: Inorganics and Solvents for Classical Analysis and Research and Part 2: Solvents and Reagents for Chromatography Applications

<h4><strong>Eight Webinars and a Roundtable with Experts from Merck</strong></h4><p><strong>Analytical Excellence Week' is organized to empower professionals in the field of analytical chemistry. Over the course of eight insightful sessions, industry experts will share their knowledge and best practices to streamline your analytical workflows and enhance your efficiency.</strong></p><p>From <strong>understanding quality grades</strong> in inorganics and solvents to <strong>exploring sustainable practices</strong> in the lab, each webinar covers essential topics that will enhance your analytical capabilities. Learn how to <strong>optimize your Karl Fischer titration</strong>, achieve precision in PFAS testing, and gain <strong>troubleshooting tips for LC-MS</strong> to effectively address common challenges and improve your laboratory results.</p><p>The sessions will also address critical aspects of <strong>safety and sustainability</strong>, ensuring you are well-equipped to handle hazardous chemicals responsibly while making strides toward greener laboratory practices.</p><p>This is an invaluable opportunity to gain insights that can significantly optimize your analytical processes. Don’t miss your chance to elevate your expertise and contribute to a more efficient and sustainable future in analytical workflows.</p><p>Each session is specifically designed to address essential topics that directly impact your daily practices, making it relevant for all professionals working in a chemical analysis laboratory.</p><h3><strong>Day 3: Wednesday, 12 February</strong></h3><h4><strong>Optimize Your Karl Fischer Titration: Expert Insights for Enhanced Results</strong></h4><p><strong>With Karl Fischer titration, you can quickly and accurately determine water content in a wide variety of samples. The accuracy of the results depends on a well-defined titration application process. Pre-titration and titer determination are essential for obtaining correct results. The main challenges arise from samples that do not dissolve or that undergo side reactions in standard solvents. Learn the measures you can take to release water from such samples and the preferred methods to use—volumetry or coulometry. Additionally, discover how digital data transfer enables efficient, fast, and secure data integration with your titrator software.</strong></p><p><strong>By attending this webinar, you will learn:</strong></p><ul><li>The key steps in the Karl Fischer titration process</li><li>The importance of accurate pre-titration and regular titer determination</li><li>Effective methods for completely releasing water from your samples</li><li>How digital data transfer can streamline and secure your daily data transfer and documentation</li><li>Practical tips for overcoming daily challenges in Karl Fischer Titration</li></ul><p><strong>Who should attend:</strong><br>This webinar will be especially valuable for individuals involved in developing Karl Fischer titration applications, performing titrations in QC labs or during in-process control, and those responsible for assessing and enhancing processes. This includes lab technicians, lab managers, and quality assurance managers across various segments.</p><blockquote><p><strong>Presenter:</strong> <i>Bettina Straub-Jubb, (Global Product Manager Analytical Technologies, Merck)</i></p></blockquote><h4><strong>Beyond Safety Standards </strong><i><strong>–</strong></i><strong> Best Practices for Safe Handling of Hazardous Chemicals</strong></h4><p><strong>This webinar illustrates the role of safety in the lab as well as the challenges that can occur while handling hazardous goods in the daily lab work and how to overcome these challenges. Rules and regulations for daily handling of hazardous goods are discussed, and options are shown on how to improve daily processes regarding personal safety. The presentation focuses on the prevention of accidents, emergency help in case of chemical spillages, and safe and reliable cleaning of lab equipment. Join the webinar for an informative overview of safety solutions and tools for maximum safety and reliability in your lab.</strong></p><p><strong>By attending this webinar, you will learn:</strong></p><ul><li>Basic safety risks and rules for daily handling of hazardous goods and options for how to improve daily processes regarding personal safety</li><li>Packaging-related safety topics</li><li>Practical guidelines for handling hazardous chemicals in case of spillages</li><li>Lab equipment cleaning know-how for reliable analytical results</li></ul><p><strong>Who should attend:</strong><br>Staff and head of chemical labs (e.g. quality control, research), as well as safety managers/safety engineers for chemical laboratories.</p><blockquote><p><strong>Presenter: </strong><i>Annett Schneider, (Global Product Manager and Lead Expert for Safety Products and Essentials, Merck)&nbsp;</i></p></blockquote>

Webinar Series & Expert Roundtable (Day 3)

Part 1: Optimize Your Karl Fischer Titration and Part 2: Beyond Safety Standards – Best Practices for Safe Handling of Hazardous Chemicals

<p><strong>Laboratory balance cleaning is extremely important for accurate weighing results and operator safety. It can be challenging but also fast and easy if you know how to do it right.</strong></p><p>A clean analytical balance or precision balance is the first step toward safe and accurate weighing. Cleaning increases user safety reduces the risk of cross-contamination, and is the most important action to prolong instrument life. Join our free live webinar to find out more about the benefits of and solutions for cleaning your balance.</p><p><strong>Program:</strong></p><ul><li>Why cleaning lab equipment is important for safety and weighing accuracy</li><li>The consequences of not cleaning your balances correctly</li><li>How often you should clean your balance</li><li>The detergents that are suitable for balance cleaning</li><li>The balance features that support fast and effortless cleaning</li></ul><blockquote><p><strong>Presenter: </strong>Adriano Guiso (Trainer, METTLER TOLEDO)</p></blockquote>

Mettler Toledo: Laboratory Balance Cleaning

Laboratory Balance Cleaning

Join our free live webinar to find out more about the benefits of and solutions for cleaning your balance.

<p><strong>Join us for a lesson in how to look at your spectra from Sue Weintraub. Using examples from her research, Sue will highlight the importance of looking at mass spectra and teach you how to get started.</strong></p><blockquote><p><strong>Presenter: </strong>Sue Weintraub (Professor, University of Texas Health Science Center at San Antonio)</p></blockquote>

Don’t Just Trust the Algorithm: Why You Need To Look at Your Spectra