<p><strong>🎤Lindsay Repka&nbsp;</strong></p><p><strong>Lindsay Repka is a chemistry professor at Middlebury College whose research explores photoredox chemistry, catalyst stability, and visible-light-driven transformations. Her lab emphasizes both mechanistic insight and hands-on student training in advanced analytical instrumentation.</strong></p><p>In this episode of Concentrating on Chromatography, we sit down with Lindsay Repka to discuss how LC-MS and GC-MS transformed her lab’s approach to photoredox chemistry.</p><p>What began as a project to develop a visible-light photocrosslinking handle unexpectedly led to a major discovery: the solvent (DMF) was reacting with the photocatalyst itself. Using high-resolution LC-MS, Lindsay’s team observed multiple solvent adducts forming — sometimes with complete catalyst consumption. That discovery reshaped their research direction.</p><p>This episode is a rare deep dive into both LC-MS and GC-MS within the same research project, showing how chromatography-driven insight can turn unexpected degradation into productive new reactivity.</p><p><strong>If you work in:</strong></p><ul><li>Photoredox chemistry</li><li>Reaction optimization</li><li>Mass spectrometry method development</li><li>Catalyst screening</li><li>Academic synthetic chemistry</li></ul><p>…this conversation will resonate.</p><h4><strong>Key Topics Covered</strong></h4><ul><li>Photocatalyst stability in DMF, DCE, and MeCN</li><li>Demethylation under mild visible-light conditions</li><li>High-resolution Q-TOF LC-MS quantitation</li><li>Internal standard methodology in GC-MS</li><li>Signal-to-noise improvement using extracted ion chromatograms</li><li>Reaction reproducibility and quality control strategy</li></ul><h3><strong>Video Transcription</strong></h3><p>In this interview, Lindsay Repka discusses the role of photoredox chemistry in method development, photocrosslinking, solvent activation, catalyst stability, and the use of LC-MS and GC-MS to understand reaction pathways.</p><h4><strong>What is photoredox chemistry?</strong></h4><p>Photoredox chemistry uses light to activate a photocatalyst, which can either donate or accept an electron from a substrate. This generates radical species that can undergo new types of reactivity.</p><p>Repka explains that one of the key advantages of photoredox chemistry is its ability to operate under mild conditions, often using visible light. This makes it attractive for sensitive molecules and biological systems, including potential applications in drug release, live-cell chemistry, and drug development.</p><h4><strong>Photocrosslinking and drug-target interactions</strong></h4><p>Repka’s group initially aimed to develop visible-light photocrosslinking methods to study drug interactions with biological targets. Photocrosslinking can covalently link a drug to its target, making it easier to identify the biological target of a compound.</p><p>Traditional photocrosslinking often uses UV light, but visible light offers milder and more biologically compatible conditions.</p><h4><strong>From photocrosslinking to solvent activation</strong></h4><p>The project shifted direction when the desired photocrosslinking reactions gave very low yields. Instead of reacting with amino acids as intended, the radical intermediate mainly reacted with itself.</p><p>The team explored different solvents to stabilize the radical, but found that some solvents, such as DMF, were not inert. Instead, the solvent reacted with the photocatalyst or radical species, revealing unexpected solvent activation chemistry.</p><h4><strong>Why LC-MS was critical</strong></h4><p>LC-MS became essential because the reaction mixtures were too complex and low-yielding for NMR or GC-MS. High-resolution LC-MS allowed the team to detect minor products, identify photocatalyst modifications, and observe solvent addition products with high confidence.</p><p>Repka emphasizes that LC-MS was both a qualitative and quantitative tool. It was used quantitatively to measure how much photocatalyst remained after light exposure, and qualitatively to identify degradation products and new reactivity pathways.</p><h4><strong>Photocatalyst stability workflow</strong></h4><p>The team developed a systematic workflow to evaluate photocatalyst stability under light exposure. Because catalyst degradation was time-sensitive, reproducibility required strict control of experimental conditions.</p><p>Key steps included using a glove box, commercial deoxygenated solvents, accurate weighing on a microbalance, controlled light exposure in a photoreactor, and temperature control with a chiller. These precautions helped reduce day-to-day variability and enabled reproducible measurements of catalyst remaining.</p><h4><strong>Quantifying catalyst remaining by LC-MS</strong></h4><p>Calibration curves were prepared using pure photocatalyst solutions and LC-MS ion areas. The team worked in the nanomolar range to maintain linearity.</p><p>Instead of relying only on total ion chromatograms, they used extracted ion chromatograms (EICs) to improve signal-to-noise for the photocatalyst signal. Baseline subtraction was also applied to reduce noise and improve quantitation at low concentrations.</p><p>Calibration curves and quality control samples were prepared and measured on the same day as the reaction samples, because LC-MS signal intensity can vary significantly from day to day.</p><h4><strong>Why GC-MS was used later</strong></h4><p>For demethylation studies, the group moved to GC-MS because the substrates and products were small and volatile enough for gas chromatography. GC-MS was better suited for rapidly screening many reaction conditions, including different photocatalysts, solvents, and oxidants.</p><p>Instead of generating full calibration curves for every experiment, the team used an internal standard to streamline analysis and determine percent conversion based on relative response factors.</p><h4><strong>Practical lessons from the project</strong></h4><p>Repka highlights the importance of careful sample preparation, especially when working with very small quantities of photocatalysts. A high-quality microbalance, stable granite platform, antistatic tools, and aluminum weigh boats improved weighing accuracy and reproducibility.</p><p>She also argues that mass spectrometry should be a standard part of photoredox method development because it can reveal minor products, support reaction optimization, and provide insights that may be difficult or impossible to obtain by NMR alone.</p><h4><strong>Final perspective</strong></h4><p>Beyond the chemistry, Repka emphasizes the importance of student training and mentoring. For her, research is not only about discovering new reactivity but also about giving students practical experience, helping them develop independence, and preparing them for future scientific careers.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Using LC-MS & GC-MS to Decode Photoredox Catalyst Stability

<p><strong>In the week of May 18, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Shimadzu Corporation: Accelerating ROI With Shimadzu Living Laboratory: Transforming Data Intelligence With AI &amp; Automated Networked Systems</strong></h5><ul><li>Tu, 19.5.2026 08:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6197">Registration</a></li></ul><p>Discover how Shimadzu and Caliber are transforming laboratory operations through AI, automation, and connected data ecosystems that unify method development, analysis, and QC workflows.</p><h5><strong>2. Thermo Fisher Scientific: Proteome Discoverer Workshop - Processing DIA data with CHIMERYS intelligent search algorithm</strong></h5><ul><li>Tu, 19.5.2026 14:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6199">Registration</a></li></ul><p>Join this Thermo Fisher Scientific workshop to learn DIA data analysis best practices in Proteome Discoverer using the CHIMERYS intelligent search algorithm and new PD 3.3 SP1 features.</p><h5><strong>3. Waters Corporation: CDMS Applied: Bridging Molecular Complexity and Clarity from Bioconjugates to Viruses</strong></h5><ul><li>Tu, 19.5.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6176">Registration</a></li></ul><p>Explore how CDMS extends mass spectrometry for complex biomolecules. Learn how it enables accurate analysis of glycoprotein vaccines and viral structures beyond native MS limits.</p><h5><strong>4. Technology Networks: Building Analytical Strategies for Scalable Antibody Development</strong></h5><ul><li>Tu, 19.5.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6184">Registration</a></li></ul><p>This webinar explores how decisions made during the investigational new drug (IND) stage can impact validation readiness, technology transfer, and regulatory success.</p><h5><strong>5. SelectScience: The proteomic LC-MS strategy revealing autoimmune mechanisms with new clarity for clinical research</strong></h5><ul><li>Tu, 19.5.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6175">Registration</a></li></ul><p>Integrated proteomics and multiomics strategies are transforming the way researchers investigate immune dysregulation and disease mechanisms.</p><h5><strong>6. Phenomenex: Ion-Pair Reversed-Phase LC Method Development for sgRNA Impurity Analysis</strong></h5><ul><li>We, 20.5.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6200">Registration</a></li></ul><p>Learn how an AQbD-driven ion-pair reversed-phase LC method enables robust sgRNA impurity profiling and high-resolution separation of closely related RNA impurities.</p><h5><strong>7. SCIEX: PFAS proven: Show me the data</strong></h5><ul><li>We, 20.5.2026 12:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6201">Registration</a></li></ul><p>Discover how PACE Analytical Services performs robust ultrashort PFAS analysis using direct injection workflows and the SCIEX 7500+ system for real-world environmental samples.</p><h5><strong>8. Technology Networks: Building Reliable Lateral Flow Assays: Real-World Case Studies Reviewed</strong></h5><ul><li>We, 20.5.2026 14:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6061">Registration</a></li></ul><p>This webinar examines practical approaches to strengthening lateral flow assay performance, with a focus on optimized sample handling and strategic material selection.</p><h5><strong>9. SCIEX: HRMS workflows for peptide impurity characterization</strong></h5><ul><li>We, 20.5.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6202">Registration</a></li></ul><p>Explore advanced HRMS and CMC strategies for peptide impurity characterization, including LC-HRMS workflows, EAD fragmentation, and regulatory considerations for GLP-1 RAs and oligonucleotides.</p><h5><strong>10. Waters Corporation: Beyond Surface Separation: Navigating Mega Molecule Sizing with Wide Pore SEC-MALS</strong></h5><ul><li>We, 20.5.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6177">Registration</a></li></ul><p>Understand how pore structure impacts SEC separations of large biomolecules. Learn how SEC-MALS enables accurate sizing, aggregation analysis, and robust method development.</p><h5><strong>11. Thermo Fisher Scientific: Driving peptide bioanalysis forward with selective MS and streamlined data workflows</strong></h5><ul><li>Th, 21.5.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6204">Registration</a></li></ul><p>Join us to learn how advanced MS³ workflows and scalable software can expand the capabilities of modern bioanalytical labs.</p><h5><strong>12. Agilent Technologies: Combining Metabolomics and CRISPRi to Understand and Engineer Bacterial Metabolism</strong></h5><ul><li>Th, 21.5.2026 13:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6203">Registration</a></li></ul><p>In this seminar, we will discuss how intracellular metabolite concentrations shape the function and robustness of metabolic networks.</p>

Webinars LabRulezLCMS Week 21/2026

A Novel Compact Multi-Reflecting Time-of-Flight Mass Spectrometer

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<p><strong>The Cary 3500 UV-Vis system implements the most recent optical components and technology to increase the speed of analysis to make one's laboratory more productive and reduce analysis backlog.</strong></p><blockquote><p><strong>Presenter:</strong> Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)</p><p>Mark, an Application Scientist for Agilent Technologies, has over 35 years of experience in molecular spectroscopy. He has held roles in Agilent's UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark's responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.</p></blockquote>

Agilent Technologies: A UV-Vis that was Designed From Top to Bottom to Meet the Needs of the Biochemistry Laboratory

A UV-Vis that was Designed From Top to Bottom to Meet the Needs of the Biochemistry Laboratory

The Cary 3500 UV-Vis system implements the most recent optical components and technology to increase the speed of analysis.

A UV-Vis that was Designed for Biochemistry Laboratory

<h4><strong>Preparing for next generation LC-MS based proteomics</strong></h4><p><strong>Presenter:</strong> Nicolai Bache (VP Proteomics research at Evosep)</p>

Evosep Biosystems: Preparing for next generation LC-MS based proteomics

Preparing for next generation LC-MS based proteomics

Get a sneak peek of what’s coming at ASMS 2025.

<h4><strong>Expand Your Expertise with Our Chromatography Webinar Series 2025</strong></h4><p>A comprehensive journey through fundamental principles, HPLC optimization, and cutting-edge mixed-mode techniques. Led by industry experts, these free sessions provide practical insights and real-world applications to enhance your analytical skills.</p><h5><strong>Session 3: Introduction to Mixed-Mode Chromatography in HPLC</strong></h5><ul><li>What is mixed-mode chromatography?</li><li>Advantages over single-mode techniques (RP, IEX, HILIC, etc.)</li><li>Mechanisms of multiple interactions: hydrophobic, ionic, hydrogen bonding, and π-π interactions</li><li>How mixed-mode selectivity can improve complex separations</li></ul><blockquote><p><strong>Presenter: </strong>Jovana Mitrovic (R&amp;D Chemist at SIELC Technologies)</p><p>Jovana holds a Master of Science in Molecular Engineering from the Pritzker School of Molecular Engineering at the University of Chicago and a Bachelor of Science in Biomedical Sciences from St. John’s University. Her background in molecular and biomedical sciences supports a multidisciplinary approach to addressing complex analytical challenges.</p></blockquote>

SIELC Technologies: HPLC Educational Webinar Series

Introduction to Mixed-Mode Chromatography in HPLC

Join us for a webinar and learn about what is mixed-mode chromatography, advantages over single-mode techniques (RP, IEX, HILIC) and other useful things.

<h4><strong>An introduction to accurate mass and Quadrupole-Time-of-Flight technology with SCIEX</strong></h4><p><strong>Until recently, nominal mass triple quadrupole technology was considered the gold standard for quantitative assays. In contrast, high-resolution mass spectrometry was primarily used for qualitative, semi-quantitative, and non-targeted workflows, such as metabolite identification, screening, and X-Omics. However, advancements in technology, including faster scan speeds and improved ion optics, have expanded the capabilities of high-resolution spectrometry. Now, it can be effectively applied to both qualitative and quantitative workflows.&nbsp;</strong></p><p>The ZenoTOF 7600 system, with its high scan rates and enhanced duty cycle at the MS2 level, bridges the gap between these approaches, offering a greater return on investment by reducing data acquisition times and maintaining sensitivity without compromising data quality. This webinar series will focus on how to transition from the Triple Quad to a high-resolution environment for different applications.</p><p>This webinar series will contain 3 parts. Each part will be 20 minutes with a 10 minute Q&amp;A.</p><h4><strong>Part 3: Alternative Fragmentation Modes – EAD, CID and more</strong></h4><ul><li>What are EAD &amp; other alternative fragmentation modes and how to do they work?</li><li>What do EAD spectra look like for different molecule types?</li><li>Benefits of EAD and when to use it with or instead of CID</li></ul><blockquote><p><strong>Presenter:</strong> Tom Ruane (Accurate Mass Workflows Specialist, SCIEX)</p><p>Tom has been working for SCIEX since 2018, having worked at Phenomenex since leaving the University of Manchester in 2015. He currently helps SCIEXs customers across Nordic countries, UK and Ireland working in life science research and drug development. In particular he has an interest in accurate mass technologies.</p></blockquote>

SCIEX: An introduction to accurate mass and Quadrupole-Time-of-Flight technology with SCIEX

Alternative Fragmentation Modes – EAD, CID and more 

Join SCIEX specialists and learn what are EAD & other alternative fragmentation modes and how to do they work.

Alternative Fragmentation Modes – EAD, CID and more

<p><strong>Get ready for a game-changing way to efficiently extract even more information from your molecules. Become a mass detection pro and drive higher productivity with less effort. &nbsp;</strong></p><p>Join us for this must-see virtual launch event and be the first to learn about the newest innovative solution from Agilent.</p><p><strong>Discover how you can:</strong></p><ul><li>Break through the limits of traditional methods</li><li>Deliver results confidently</li><li>Radically simplify user training and daily operation</li></ul><p>Whether you are a part of a research, development, or QA/QC analysis group, Agilent is ready to take your lab to the next level.</p><p><strong>Don’t miss your chance to ride the wave of innovation.</strong></p>

Agilent Technologies: Ride the Wave of Innovation

Ride the Wave of Innovation

Join must-see virtual launch event and be the first to learn about the newest innovative solution from Agilent.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Capillary scale liquid chromatography (LC) offers separations comparable to the more traditional analytical scale at a fraction of the solvent consumption. In this webinar, learn how your lab can benefit from a compact capillary scale LC instrument that can be hand-carried anywhere and used on-the-spot.</strong></p><p>In order to be considered as a viable replacement for larger scale systems, a system must demonstrate its robustness and repeatability. Key repeatability metrics will be discussed, including interday and intraday repeatability, and injection carryover. Discover how these metrics provide valuable insights to those looking to integrate capillary scale separations into their workflows.</p><h5><strong>Key learning objectives</strong></h5><ul><li>See how capillary scale chromatography can help your lab save solvent and sample</li><li>Learn about compact capillary scale instrumentation</li><li>View methods for the quantification of system repeatability and discover how the Axcend Focus LC® performs</li></ul><h5><strong>Who should attend?</strong></h5><ul><li>Chemists, analytical researchers, lab directors and managers, VPs and directors of QC/QA, lab owners, and academics.</li></ul><h5><strong>Certificate of attendance</strong></h5><ul><li>If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</li><li>If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Samuel Foster (Application Scientist, Axcend)</p><p>Dr. Samuel Foster completed his Ph.D. in Pharmaceutical Chemistry from Rowan University in 2025. His research has focused on the development and application of capillary scale liquid chromatography instrumentation. He currently works at Axcend as an application scientist focusing on the development of chromatographic workflows for a variety of analyte classes including oligonucleotides, monoclonal antibodies, and drugs of abuse.</p><p><strong>Moderator:</strong> Sarah Thomas (Associate Editor, SelectScience)</p></blockquote></div></div></div>

SelectScience: Save solvent and samples with compact capillary scale LC

Save solvent and samples with compact capillary scale LC

Join this educational webinar where Senior Product Manager (Thermo Fisher Scientific) will introduce NanoDrop™ Ultra for nucleic acid quantification

Save Solvent and Sample with Capillary Scale LC

<p><strong>As global awareness of PFAS contamination grows, regulatory scrutiny continues to intensify. In this session, Dr. Rainer Lohmann—Director of the University of Rhode Island’s STEEP Superfund Research Center—will examine how passive sampling strategies can support robust PFAS monitoring in complex environmental matrices. Drawing from real-world studies, Lohmann will explain how these tools help overcome persistent challenges in detecting and quantifying PFAS across air and water systems, and what scientists need to know to stay ahead of evolving regulatory demands.</strong></p><p>Analyzing PFAS in complex environmental matrices can often impact laboratories with time-consuming sample preparation and contaminated LC/MS/MS systems. The PerkinElmer QSight 500 LC/MS/MS System allows for minimized sample preparation and system contamination thanks to its StayClean Technology, offering unmatched robustness, even with complex matrices.</p><p>By adding the use of dedicated PTFE-free consumables kits and EPA-compliant software reporting features, the QSight 500 LC/MS/MS System helps labs streamline PFAS workflows. Join this presentation for real-life examples and much more.</p><h5><strong>By attending this presentation, you will:</strong></h5><ul><li>Discover key regulatory developments shaping PFAS monitoring requirements.</li><li>Explore passive sampling techniques for PFAS detection in air and water.</li><li>See real-world examples illustrating challenges and solutions for testing diverse environmental matrices.</li><li>Understand the challenges of analyzing PFAS in heavily contaminated environmental samples such as soil, sludge, and wastewater and their impact on lab efficiency and instrument performance.</li><li>Discover how the QSight® 500 LC/MS/MS System’s StayClean™ Technology minimizes system contamination and maintenance, ensuring reliable PFAS analysis in even the dirtiest matrices.</li><li>Learn how to enhance PFAS workflow efficiency through the use of PTFE-free consumables and EPA-compliant software, reducing prep time and ensuring accurate, streamlined reporting.</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Rainer Lohmann (Professor, University of Rhode Island’s Graduate School of Oceanography)</p><p>Dr. Rainer Lohmann is a Professor at the University of Rhode Island’s Graduate School of Oceanography. He obtained a degree in Chemical Engineering and a Doctorate in Environmental Science. He is Director of the URI-led Superfund Research Program Center on the Sources, Transport, Exposure and Effects of PFAS (STEEP). His research focuses on the detection, bioaccumulation, transport, and fate of anthropogenic pollutants in the environment, often relying on passive samplers in the process. Since 2000, he has published &gt; 200 publications and book chapters.</p><p><strong>Presenter:&nbsp;</strong>Tom Hey (Global Environmental Market Leader, PerkinElmer)</p><p>Tom is Global Market Leader for the environmental segment at PerkinElmer, leveraging his sales and management experience from Key Account Manager and Business Leader positions since joining in 2015. Holding a Ph.D. in Inorganic Chemistry from the University of Bristol, his problem-solving skills are leveraged when figuring out environmental solutions. His passion lies in enhancing PerkinElmer's offerings, particularly in tackling environmental challenges like PFAS and microplastics.</p></blockquote>

Separation Science: Experience Maximized Productivity for PFAS Analysis, Even with Complex Matrices

Experience Maximized Productivity for PFAS Analysis, Even with Complex Matrices

Learn how to overcome persistent challenges in detecting and quantifying PFAS with the LC/MS/MS

PFAS Analysis in Complex Matrices

<p><strong>Accurate protein concentration measurement is critical in biochemical and biophysical research. A powerful UV-Vis spectrophotometer can streamline this process, enabling rapid and precise determination of protein extinction coefficients. Intuitive software allows users to simplify extinction coefficient calculations using the Beer-Lambert law equation—eliminating complexity with just one click.</strong></p><p>Join us for an insightful webinar on how the Cary 3500 UV-Vis Multicell Pelter streamlines this process, enabling rapid and precise determination of protein extinction coefficients at 280 nm.</p><p>Learn how this innovative system supports Biopharma applications, including the analysis of innovator and biosimilar proteins.Additionally, explore how built-in calibration curves facilitate monoclonal antibody (mAb) concentration estimation during bioprocessing.</p><p>This session will provide practical insights, making it a must-attend event for researchers, analysts, and quality control professionals seeking to enhance their workflows with cutting-edge UV-Vis technology.</p><h4><strong>What you will learn:</strong></h4><ul><li>Understand the fundamentals of protein extinction coefficients and concentration analysis</li><li>Explore the Cary Workstation software features that enable one-click calculation of protein extinction coefficients</li><li>Learn how the Cary 3500 UV-Vis Multicell Pelter optimizes efficiency by simultaneously measuring multiple samples</li><li>Discover built-in software capabilities, including calibration curve development and concentration estimation for bioprocessing applications</li></ul><h4><strong>Who Should Attend:</strong></h4><ul><li>Analytical scientists and technicians using UV-Vis technologies</li><li>Researchers working with proteins, peptides, or biopharmaceuticals</li><li>Quality control specialists in biopharma and related industries</li><li>Laboratory managers looking to enhance analytical efficiency and accuracy</li></ul><blockquote><p><strong>Presenter</strong>: Aveline Neo, PhD (Solution Scientist, Agilent Technologies, Inc.)</p><p>Aveline Neo boasts an impressive background in biology and analytical science. She earned her PhD from the National University of Singapore and began her career at Agilent in 2012, where she focused on lipidomics using liquid chromatography and mass spectrometry.</p><p>After completing post-doctoral research at the Babraham Institute in Cambridge, she returned to Agilent as a solution scientist in the Global Solution Development Center. Over the years, she has contributed to various projects involving recombinant proteins, alternative proteins, mRNA, and monoclonal antibodies.</p><p>Aveline has demonstrated expertise in analytical techniques, including liquid chromatography, mass spectrometry, and spectroscopic methods such as UV-Vis, FTIR, and Raman.</p><p><strong>Moderator</strong>: Michelle Taylor (Editor-in-Chief, Labcompare | Laboratory Equipment)</p></blockquote>

Agilent Technoloies: Simplify Your Workflow: Rapid and Efficient Protein Analysis with UV-Vis Spectroscopy

Simplify Your Workflow: Rapid and Efficient Protein Analysis with UV-Vis Spectroscopy

How the Cary 3500 UV-Vis Multicell Pelter streamlines this process, enabling rapid and precise determination of protein extinction coefficients at 280 nm

Protein Analysis with UV-Vis Spectroscopy

<p><strong>This month, our seminar series features cutting-edge proteomics research from leading scientists in Canada and the U.S. We are pleased to welcome Dr. Jennifer Geddes-McAlister (University of Guelph), who will discuss a proteomics-driven approach to fungal disease, from prevention to treatment, and Dr. Ben Garcia (Washington University in St. Louis), who will present on using quantitative proteomics to investigate epigenetic mechanisms in human health. Don’t miss this exciting seminar — register for free today!</strong></p><blockquote><p><strong>Presenter: </strong>Dr. Jennifer Geddes-McAlister (University of Guelph)</p><ul><li><strong>Talk: </strong>From prevention to treatment: a proteomics-driven approach to fungal disease</li></ul><p><strong>Presenter: </strong>Dr. Ben Garcia (Washington University)</p><ul><li><strong>Talk: </strong>Quantitative proteomics for understanding epigenetic mechanisms in human disease</li></ul></blockquote>

US HUPO: US HUPO-CNPN Proteomics Spotlight

US HUPO-CNPN Proteomics Spotlight

Join us for top-tier proteomics insights from Dr. Geddes-McAlister and Dr. Garcia. Fungal disease & epigenetics — free seminar, register today

<h4><strong>The Problem:</strong></h4><p>Desalting and quantifying nucleic acids is essential for ensuring accuracy and efficiency for downstream assays, but the process can be challenging. Manual methods of desalting are time-consuming and costly. Fluorescence-based methods of quantification require standards, dyes and dilutions while the accuracy of traditional UV absorbance is affected by impurities or varying sequences.</p><h4><strong>The Solution:</strong></h4><p>Unagi makes desalting a breeze. Simply load up to 8 samples of 8 mL and step away while Unagi removes solvents like ACN, salts and small molecules. End up with a purified product in any buffer, that can even be concentrated up to 16-fold.<br><br>Lunatic guarantees accurate UV/Vis quantification from just 2 μL of sample - dye and dilution free!</p><h4><strong>The Proof:</strong></h4><p>Tune in to see how simple it is to set up a desalting concentration run on Unagi and how Lunatic quickly and accurately quantifies nucleic acids with different sequences.</p><h4><strong>Learning Objectives</strong></h4><ul><li>Discover how buffer exchange can make desalting plug-and-play</li><li>How to use UV/Vis to quantify DNA and RNA, dye-free</li><li>Conclude that desalting, concentrating and quantifying nucleic acids can be done with minimal hands-on time</li></ul><blockquote><p><strong>Presenter: </strong>Kevin Lance, PhD (Director, Product Management, Unchained Labs)</p><p>Kevin Lance directs product management and covers the Stunner portfolio globally at Unchained Labs. His expertise includes biophysical characterization of viral vectors, lipid nanoparticles, proteins, and nucleic acids. Prior to Unchained Labs, his research experience covered the fields of sustained antibody delivery and nanotechnology. He earned his Ph.D. in Bioengineering from the joint UC Berkeley – UCSF Bioengineering Graduate Program.</p></blockquote>

Unchained Labs: Embrace simple nucleic acid desalting and quantification with Unagi and Lunatic

Embrace simple nucleic acid desalting and quantification with Unagi and Lunatic

Learn how Unagi and Lunatic streamline nucleic acid desalting and quantification—fast, accurate, dye-free, and with minimal hands-on time.

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Embrace simple nucleic acid desalting and quantification with Unagi and Lunatic

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