Modernizing Routine HPLC Methods: Aligning Productivity, Compliance, and Sustainability

<h3>How to Easily Adapt Your Existing HPLC Methods</h3>Developing and optimizing an HPLC method is essential for achieving reliable separations, accurate quantification, and reproducible analytical results. As chromatography technology advances, one of the most common optimization strategies is transferring established methods to modern columns with smaller dimensions.Although the benefits are clear, many analysts hesitate when it comes to modifying a validated method.<blockquote>Questions quickly arise: How should the flow rate be adjusted? What injection volume is appropriate? Does the gradient need to be recalculated? Will the retention times change?</blockquote>Even with the necessary equations available, performing all these calculations manually can be time-consuming and prone to errors. Instead of simplifying the workflow, method transfer often becomes an unnecessary obstacle.Fortunately, there is an easier solution.<h4>Simplify Your HPLC Method Transfer</h4>Moving an existing method to a different column does not have to be complicated.The KNAUER HPLC Method Converter is designed to automate the calculations required for method transfer. Simply enter the parameters of your current method, including the original column dimensions, and specify the dimensions of the new column—length, internal diameter, and particle size. The software instantly calculates the recommended flow rate and injection volume for the new conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_7f7c19f7a3.webp" alt="KNAUER: HPLC Method Converter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_7f7c19f7a3.webp 210w," sizes="100vw" width="1022" height="759">The next step is equally straightforward. Copy the original gradient profile into the converter, and the software automatically generates the corresponding gradient program for the new column.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_2_8ba46e78bc.webp" alt="KNAUER: HPLC Method Converter - Gradients" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_2_8ba46e78bc.webp 195w," sizes="100vw" width="1440" height="1150">For even greater confidence, the converter also allows you to enter chromatographic peak information. Based on these data, it predicts the expected retention times under the new conditions. While experimental verification on the HPLC system is still recommended, these predictions provide an excellent starting point for method optimization.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_3_34f16bb3bf.webp" alt="KNAUER: HPLC Method Converter - Chromatograms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_3_34f16bb3bf.webp 196w," sizes="100vw" width="1024" height="814">The converter was developed with one primary objective: making HPLC method adaptation faster, easier, and more reliable while reducing the effort required from the user. Its intuitive interface, modern design, and automatic calculations help save valuable laboratory time—and it is available free of charge.<h4>Modernize Your Methods and Improve Laboratory Efficiency</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_4_31e7188db4.webp" alt="KNAUER: Modernize Your Methods and Improve Laboratory Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_4_31e7188db4.webp 245w," sizes="100vw" width="720" height="449"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_5_815a7bf889.webp" alt="KNAUER: HPLC Method Converter - Results" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_5_815a7bf889.webp 194w," sizes="100vw" width="1440" height="1157">One of the major advantages of the KNAUER HPLC Method Converter is its compliance with USP General Chapter &lt;621&gt;.For pharmaceutical laboratories, chromatographic methods must comply with pharmacopeial requirements that ensure product quality and patient safety. Historically, many USP methods were developed using larger HPLC columns that no longer reflect the capabilities of modern chromatography systems.Current USP, European Pharmacopoeia (Ph. Eur.), and Japanese Pharmacopoeia (JP) guidelines now permit specific adjustments to column dimensions and particle size for both isocratic and gradient methods. These changes are allowed provided that defined relationships between chromatographic parameters are maintained.The KNAUER Method Converter automatically checks whether the selected column dimensions remain within the permitted USP limits. If the proposed changes comply with the guidelines, the software confirms that the method can be transferred while remaining pharmacopeia compliant. This removes uncertainty from the method conversion process and helps laboratories modernize their analytical workflows with confidence.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_6_c9d1e28c54.webp" alt="Unsplash: Illustrative picture" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_6_c9d1e28c54.webp 245w," sizes="100vw" width="720" height="449"><h4>Increase Productivity While Reducing Solvent Consumption</h4>Updating HPLC methods is not only about shortening analysis times—it also supports more sustainable laboratory practices.Using shorter columns together with optimized flow rates significantly reduces solvent consumption, lowering operating costs and decreasing the amount of hazardous chemical waste generated during routine analyses. Faster separations also improve instrument throughput, enabling more samples to be processed within the same amount of time.Modernizing chromatographic methods therefore delivers a dual benefit: greater laboratory productivity and a smaller environmental footprint. With only a few simple adjustments, laboratories can improve efficiency while supporting more sustainable analytical workflows.

HPLC method conversion free and easy

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 6th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by Agilent Technologies and Metrohm and posters by Shimadzu/ASMS, Thermo Fisher Scientific/ASMS and Waters Corporation/ASMS!<h3>1. Agilent Technologies: Determination of 41 Per- and Polyfluoroalkyl Substances (PFAS) in Cosmetics</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_captiva_emr_pfas_food_ii_cartridge_5994_9111en_agilent_be91861646.pdf">Full PDF for download</a></li></ul>Per- and polyfluoroalkyl substances (PFAS) are synthetic compounds composed of highly stable carbon–fluorine (C–F) bonds, giving them exceptional thermal and chemical stability, leading to poor environmental degradability. PFAS have been used in cosmetics to provide water resistance, oil repellency, or application smoothness. The unintentionally presented PFAS residues in raw materials may remain in finished cosmetic products, and can be absorbed through the skin. Lip products and spray type products can also lead to inhalation or oral exposure to residues.1,2&nbsp;Agilent Captiva EMR PFAS Food cartridges are designed to provide streamlined and comprehensive matrix removal after QuEChERS extraction for complex sample matrices, demonstrating effective matrix cleanup efficiency and excellent performance for quantitative PFAS analysis.3,4 In this study, we performed QuEChERS extraction followed by cleanup with Captiva EMR PFAS Food II cartridges for PFAS analysis in five types of cosmetics.<h4>Experimental</h4><h5>Equipment and materials&nbsp;</h5>The study was performed using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III">Agilent 1290 Infinity III LC</a> system consisting of an Agilent 1290 Infinity III high-speed pump (G7120A), an Agilent 1290 Infinity III multisampler (G7167B), and an Agilent 1290 Infinity III multicolumn thermostat (G7116B). The LC system was coupled to an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6495D">Agilent 6495D triple quadrupole LC/MS system</a> (G6495D) equipped with an Agilent Jet Stream Electrospray ion source. Agilent MassHunter Workstation software was used for data acquisition and analysis.&nbsp;The 1290 Infinity III LC system was modified using an Agilent InfinityLab PFAS Analysis HPLC conversion kit (part number 5004-0006), including an Agilent InfinityLab PFC delay column, 4.6 × 30 mm (part number 5062-8100). Chromatographic separation was performed using an Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=ZORBAX+RRHD+Eclipse+Plus">ZORBAX RRHD Eclipse Plus</a> C18, 95 Å, 2.1 × 100 mm, 1.8 µm (part number 959758-902).<h4>Results and discussion&nbsp;</h4><h5>Chromatography and calibration curve linearity&nbsp;</h5>Figure 2 shows the overlaid chromatograms for 41 PFAS calibration standards, including 0.05, 0.1, 0.2, 0.5, 1.0, 2.0, 5.0, and 10.0 ng/mL in ACN. The table on the right shows the peaks identification and corresponding retention time. Across 41 PFAS compounds, R² values ranged from 0.9940 to 0.9998, indicating excellent linearity for all analytes' calibration curves across the calibration range of 0.05 to 10.0 ng/mL.<h5>PFAS detection in real cosmetic samples&nbsp;</h5>Of the 15 cosmetic products assessed, one eyeshadow sample was detected with a trace level of positive PFAS analytes, including PFHeA, PFHpA, PFNA, and PFOA, at about 0.1 ng/g in the sample. Figure 5 shows the method blanks and positive detection of PFHpA and PFNA in this eyeshadow&nbsp;product.<h4>Conclusion&nbsp;</h4>A simplified, rapid, and reliable method using QuEChERS extraction followed by EMR mixed-mode passthrough cleanup using the Agilent Captiva EMR PFAS Food II cartridge and LC/MS/MS detection was developed for 41 PFAS analysis in cosmetics.<h3>2. Metrohm: brightRC – Advanced brightener analysis with a response curve</h3>Next-level brightener determination in nonlinear systems<ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_v_242_download_83376b2923.pdf">Full PDF for download</a></li></ul><h4>WHAT IS BRIGHTRC?&nbsp;</h4>brightRC is a response curve calibration approach for the determination of brightener-type additives by CVS or CPVS (Cyclic Pulse Voltammetric Stripping), hence the name «brightRC». This technique is suitable to measure brightener content in copper baths for plating. The method is based on signal normalization using the electrolyte value, which is the response for the virgin makeup solution (VMS) saturated with suppressor, and brightener defined as Q0 in the viva software. All recorded signals are divided by Q0 and the normalized values are plotted against concentration to generate an external calibration curve. This calibration can then be used for routine sample determination without repeated standard additions.&nbsp;The sample is simply measured using the same method as the calibration, signals are normalized and evaluated using the mathematically fitted calibration curve. By decoupling calibration from sample measurement, brightRC removes the need for repeated standard additions with each sample measurement, enabling faster and more efficient routine analysis.&nbsp;The brightRC calibration approach supports flexible regression models including quadratic and nonlinear regression. This allows reliable quantification of additive systems that do not exhibit a wide linear working range or exhibit nonlinear response characteristics, thereby expanding the range of applicable brightener chemistries.&nbsp;One important detail that further differentiates brightRC from the MLAT approach relates to normalization of the signal for calibration and sample analysis. This normalization approach improves measurement robustness as variations in absolute signal intensity naturally occurring over the working day are mathematically compensated.<h4>HOW DOES BRIGHTRC WORK IN PRACTICE?&nbsp;</h4>The brightRC workflow separates calibration and sample analysis. It can be implemented in different setups (from manual to fully automated) depending on the additive system and concentration range. After recording the electrolyte value, calibration standards are measured to establish the calibration curve, otherwise known as the response curve. Once the calibration is stored, samples are determined with the same CVS or CPVS method, without further standard additions. The sample concentration is automatically calculated from the normalized signal using the calibration curve.&nbsp;For sample measurement, viva 4.0 provides two practical approaches:&nbsp;<ol><li>«brightRC with solution exchange»&nbsp;</li><li>«brightRC with sample dilution» Both variants follow the same calibration principle and differ only in the sample handling strategy.</li></ol><h4>WHEN TO USE BRIGHTRC?&nbsp;</h4>brightRC is ideal for routine brightener control in stable plating baths where high sample throughput is required. It is particularly suitable when matrix conditions are consistent and electrode fouling is limited. The approach is especially advantageous for additive systems exhibiting nonlinear signal behavior. If strong matrix variations, significant breakdown products, or severe electrode fouling are expected and a linear correlation between signal and analyte concentration is guaranteed, standard addition techniques such as MLAT are preferred because they may provide additional robustness.<h3>3. Shimadzu / ASMS: PFAS Analysis of Leachate: Evaluating Key Sample Preparation and LC–MS/MS Parameters for Improved Performance</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/WP_255_PFAS_Analysis_of_Leachate_Evaluating_Key_Sample_Preparation_and_LC_MSMS_Parameters_for_Improved_Performance_1114ee264c.pdf">Full PDF for download</a></li></ul>Landfill leachate is a highly complex and variable matrix that poses significant challenges for the analysis of per- and polyfluoroalkyl substances (PFAS). Although U.S. Environmental Protection Agency Method 1633A provides a standardized framework for PFAS quantitation,1 analytical performance remains strongly influenced by sample preparation, chromatographic separation, and mass spectrometric conditions. Landfill leachate samples were extracted using WAX SPE following EPA 1633A and analyzed by LC–MS/MS using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8060">Shimadzu LCMS-8060</a> under negative ESI conditions. This study evaluates the key factors governing LC–MS/MS sensitivity, signal response, and reproducibility in landfill leachate, including a systematic assessment of SPE, chromatographic behavior, and ionsource conditions across PFAS classes (Figure 1).<h4>Results&nbsp;</h4>Chromatographic performance was evaluated across four C18 columns (A–D) using peak area, height, asymmetry, and HETP. Two columns produced a higher signal and improved peak shape, while the remaining columns showed greater efficiency. Under repeated batches followed by storage in acetonitrile, the Shimadzu GIST column produced a consistent signal (≤15% change), whereas another column showed 20–50% signal loss, particularly for long-chain PFAS and precursors, indicating greater susceptibility to matrix accumulation (Figure 2).<h4>Conclusion&nbsp;</h4><ul><li>PFAS analytical performance was dependent on compoundclass-dependent extraction, chromatography, and ionization behavior.&nbsp;</li><li>GIST demonstrated lower signal variability under repeated matrix exposure, with lower signal degradation than alternative C18 phases.</li><li>Neutral PFAS showed poor retention on WAX sorbents, requiring optimization beyond standard EPA 1633A extraction conditions.&nbsp;</li><li>PFCA response was more influenced by gas flow conditions, whereas PFSA response was more sensitive to source temperature conditions.</li></ul><h3>4. Thermo Fisher Scientific / ASMS: Ultra-high throughput proteomics at &gt;500 samples/day using advanced <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Vanquish Neo UHPLC</a> methods with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959">Orbitrap Astral Zoom MS</a></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004708_lsms_ultra_high_throughput_vanquish_astral_proteomics_po004708_asms2026_na_en_f6499c3eec.pdf">Full PDF for download</a></li></ul>High-throughput mass spectrometry is increasingly critical for large-cohort proteomics, population-scale studies, and screening applications. While established LC–MS platforms routinely support several hundred samples per day, further increases in throughput are often limited by chromatographic stability, robustness, and reproducibility under ultra-short gradient conditions, as well as the acquisition speed of the mass spectrometer. Here, we describe an advanced ultra-high-throughput LC–MS methodology developed on the Thermo Scientific Vanquish Neo UHPLC system and Thermo Scientific Orbitrap Astral Zoom mass spectrometer. The goal of this work was to extend the practical throughput limits of LC–MS while achieving deep proteome coverage and maintaining run-to-run reproducibility.<h4>Materials and methods</h4><h5>LC-MS analysis&nbsp;</h5>Samples were separated on a Thermo Scientific 150 µm × 70 mm C18 column (prototype, not commercially available yet) operated at 4 µL/min using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Vanquish Neo UHPLC system</a> in NanoCap Trap&amp;Elute configuration at room temperature. Prototype methods allowing for reduced LC duty cycles (approx. 1 min of sample pickup/loading and integrated trap-column offline wash) were applied, the standard gradients were adjusted to 1 – 3 min. The Thermo Scientific PepMap Neo 5 µm C18 300 µm × 5 mm cartridge was used as trap column and the separation column connected with a 15 µm ID capillary Thermo Scientific EASY-Spray emitter (ES994) to an EASY-Spray source. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959">Orbitrap Astral Zoom MS</a> (ICSW AST 2.2) was operated in a dataindependent acquisition (DIA) mode, MS method parameters are given in Table 1. Comparative data for the chemoproteomics samples at lower throughputs (60-300 SPD) were acquired using an EASY-Spray 150 µm × 150 mm C18 column (ES906), as described before.&nbsp;<h5>Data analysis&nbsp;</h5>The raw data files were processed with Spectronaut® 20.5 software (Biognosys AG), <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782">Thermo Scientific Proteome Discoverer </a>3.3 SP1 with CHIMERYS 5.0 or DIA-NN Enterprise 2.3.2 (Aptila Biotech GmbH) nodes using default settings as specified in the individual figures. The data was searched against the Homo sapiens SwissProt database (TaxID=9606: 42,252 sequences) and contaminants FASTA file. Peptide numbers correspond to unique stripped peptides<h4>Conclusions&nbsp;</h4><ul><li>The advanced Vanquish Neo UHPLC methodology in combination with the Orbitrap Astral Zoom MS delivered highly reproducible, deep and precise proteome profiling across ultra-high-throughput regimes (600 SPD) exceeding current field benchmarks.&nbsp;</li><li>The workflow enables rapid chemoproteomic screening, as demonstrated with activity-based protein profiling (ABPP) samples, reliably identifying enriched putative target proteins of an antibiotic small molecule probe.</li></ul><h3>5. Waters Corporation / ASMS: Assessing Relative Response of Four European-Regulated PFAS in Human Serum Using Cyclic Ion Mobility MS</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009410_c98e7c87ec.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster presents an LC-Cyclic-IMS-MS approach for assessing four European-regulated PFAS—PFOS, PFOA, PFNA, and PFHxS—in human serum. The study focuses on environmental exposure monitoring in Ghanaian firefighters and e-waste handlers, using ion mobility mass spectrometry to add separation power and improve identification confidence in complex biological matrices. The work is motivated by increasing regulatory concern around PFAS toxicity, bioaccumulation, and the need for reliable exposure assessment in human biofluids.The experimental workflow combined reversed-phase LC with a quadrupole-cyclic ion mobility-time-of-flight MS system (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/202">SELECT SERIES Cyclic IMS</a>). Human serum samples were prepared using SPE µElution plates with a polymeric reversed-phase/weak anion exchange sorbent. The LC system was modified with PFAS-free components and an isolator column to reduce background contamination, which is especially important for trace-level PFAS analysis. Identification was supported by retention time, accurate mass, isotopically labelled internal standards, and collision cross section values.The results showed detectable levels of the four regulated PFAS in both occupational groups, with a combined detection rate of 83% across human serum samples and NIST reference standards. Firefighter samples generally showed higher concentration ranges than e-waste handler samples, especially for PFOS, PFOA, PFNA, and PFHxS. For example, PFNA reached up to 5.713 ng/mL in firefighter serum, compared with up to 1.513 ng/mL in e-waste handler serum.Overall, the poster concludes that LC-Cyclic-IMS-MS provides a highly specific strategy for PFAS analysis in complex biological samples. Ion mobility and CCS values improve confidence in trace-level identification and help distinguish target PFAS from co-eluting matrix background. The authors suggest that this approach can support both targeted PFAS monitoring and broader non-targeted screening of known and emerging PFAS, while also helping future studies connect exposure data with environmental fate and potential toxicological effects.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 28, 2026

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A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Whereas the focus of Part I of this two part series was on entry level and mid-performance instrumentation, Part II will focus on the high end UV-Vis-nir instrumentation and the accessories which allow for a more complex measurements to be made. Measurements such as diffuse reflection and Absolute Reflectance at angles will be examined.<blockquote>Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)</blockquote><blockquote>Mark, an Application Scientist for Agilent Technologies, has over 30 years of experience in molecular spectroscopy. He has held roles in Agilent's UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark's responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.Presenter: Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.</blockquote>

Agilnt Technologies: UV-Vis Instrumentation : From Routine to Advanced Applications Part II

UV-Vis Instrumentation : From Routine to Advanced Applications Part II

We will focus on the high end UV-Vis-nir instrumentation and the accessories which allow for a more complex measurements to be made.

UV-Vis Instrumentation : From Routine to Advanced Apps II

<h4>European Free Educational Webinar Series</h4>
Let&#39;s go back to the basics of Chromatography, Mass Spectrometry, Data Analysis and Trouble-shooting: understanding the importance of the basics will enable you to be more effective in your daily laboratory life.
<h4>EPISODE 8</h4>
Troubleshooting your LC and MS Systems
What will you learn?
<ul>
<li>Troubleshooting your LC and MS Systems in your Laboratory</li>
<li>When to start thinking about Instrument Replacement</li>
</ul>
<blockquote>
Prezenter: Andreas Goertges (Senior Service Sales Account Manager, Waters)
</blockquote>
<blockquote>
My name is Andreas and at Waters I am responsible for the service contracts business in Germany North. After my education and during my studies of Analytical Chemistry in Heerlen NL, I worked in a GMP regulated QC lab at our customer Grünenthal.
</blockquote>
<blockquote>
When I started with Waters in 2010, I worked as a Field Service engineer for LC and MS as well as with Autopurification systems. With this background, I know our instruments by heart from a customer perspective as well as an engineer.
</blockquote>
<h4>What&#39;s next?</h4>
Full Webinar Series Episodes List is available <a href="https://event.on24.com/wcc/r/4551012/F69479929A905500D8B21508527807E5/5385652">here</a>
Back to Basics - Sample Preparation: Theory and Overview
<ul>
<li>Tuesday, May 07, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Sample Preparation: Tips &amp; Tricks to make it simple
<ul>
<li>Wednesday, June 05, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Theory of Liquid Chromatography
<ul>
<li>Wednesday, July 03, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Basics of Liquid Chromatography
<ul>
<li>Tuesday, September 10, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Analytical Method Development
<ul>
<li>Wednesday, October 02, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - The importance of Compliance
<ul>
<li>Tuesday, October 22, 2024, 11:00 AM CEST</li>
</ul>
Back to Basics - Introduction to Mass Spectrometry
<ul>
<li>Wednesday, November 13, 2024, 11:00 AM CET</li>
</ul>
Back to Basics - Troubleshooting your LC and MS Systems
<ul>
<li>Tuesday, December 10, 2024, 11:00 AM CET</li>
</ul>
Not sure to be able to attend live sessions?
Register today and watch the webinars on-demand

Waters Corporation: Back to Bacisc Free Educational Webinar Serries

Troubleshooting your LC and MS Systems

LC and MS systems troubleshooting and when to start thinking about instrument replacement.

Gain insights into how delay columns, essential to control the presence of PFAS in the laboratory, function. And understand how different delay columns impact method performance.We will host a Q&amp;A session after the presentation to address any questions or comments.<blockquote>Presenter: Landon Wiest, PhD (Product Manager - LCMS, Shimadzu Scientific Instruments)Dr. Landon Wiest is the Product Manager for LCMS at Shimadzu Scientific Instruments, leading a team of scientists who focus on applications development customer care. He has extensive experience in method and product development, research project management, and customer applications, with a strong background in liquid chromatography. At Restek Corporation, Landon led technology development teams and advanced new particle technologies, while also publishing application notes and creating educational content for webinars and short courses.He earned a Ph.D. in Analytical Chemistry from Brigham Young University, where he developed patented diamond/carbon-based chromatographic particles. Recognized for his contributions to chromatography, Landon was nominated for the Csaba Horváth Award. Fluent in Danish and knowledgeable in German, Landon excels at communicating complex scientific concepts and is dedicated to advancing scientific knowledge. Presenter: Ruth Marfil-Vega, PhD (Sr Market Manager – Environmental, Shimadzu Scientific Instruments)Dr. Ruth Marfil-Vega has 20 years of expertise in environmental chemistry and engineering in the US and Europe. She has worked with the EPA and the utilities industry to design and execute comprehensive solutions for addressing new environmental and water quality issues, including PFAS, regulated and unregulated DBPs, and other emerging contaminants. Since Ruth joined Shimadzu five years ago, she has continued to work with environmental stakeholders to ensure that analytical instrumentation and workflows support their success. She earned a BSc in Chemistry from the University of Valladolid (Spain) and a PhD in Environmental Science and Engineering from the University of Cincinnati.</blockquote><h3>PFAS in Focus</h3>Welcome to PFAS in Focus, a comprehensive and exclusive webinar series sponsored by Shimadzu, covering the basics and beyond about the analysis of Per- and Polyfluoroalkyl Substances (PFAS) in the environment.Join our experts, live or on-demand after the original air date, to demystify the challenges of PFAS analysis and gain the insights you need to:<ul><li>Manage PFAS contamination in your laboratory.</li><li>Master standardized methods for PFAS testing.</li><li>Discover new analytical workflows to maximize the performance of your LC-MS/MS for PFAS analysis.</li></ul>Who should attend:<ul><li>Environmental industry professionals and academics currently working on PFAS analysis or looking to establish the application in their labs.</li><li>Representatives from drinking and wastewater utilities.</li><li>LC–MS users from any field interested in expanding their applications to include PFAS testing.</li></ul>

Mastering PFAS Control: Transform Your Laboratory Practices

Gain insights into how delay columns, essential to control the presence of PFAS in the laboratory, function.

Join us for our winter school dedicated to accelerating your knowledge of innovative triple quad technologies and learn the fundamentals and theory of mass spectrometry. This winter, we're offering a 30-minute webinar designed to fit into your schedule.<h5>Learn more about:</h5><ul><li>The fundamentals and theory of mass spectrometry.</li><li>The ease of getting from data to results.</li><li>Recent innovations in SCIEX instrumentation: SCIEX 7500+ system.</li></ul>Event 1: December 10, 2024 10:00 - 10:30 CETEvent 2: December 17, 2024 10:00 - 10:30 CETEvent 3: January 7, 2025 10:00 - 10:30 CET

Winter school: LC-MS essentials

Master the essentials of triple quad technology with our live webinars this winter.

Session 1:The fundamental unit of a living organism is the single cell. What are the benefits and issues with pushing towards single cell measurements? The overarching goal is to provide an understanding of why discovery based single cell analysis is important and achievable. Several example applications highlight single cell metabolites including amino acids, small bio-active neuropeptides and neurotransmitters. Metabolites in individual cells were characterized using capillary electrophoresis (CE) with MRM MS with the QqQ-MS. The sample preparation steps were performed in vial inserts in the CE system to reduce the solution requirements of the autosampler to ~2 µL. Other changes to the system include a nanointerface and a mechanically tapered capillary tip for optimized detectability. The CE-nanoESI-QqQ MS system demonstrated detection limits at the single attomole level for a range of amino acids and transmitters. We characterize a range of cells including Aplysia californica neurons, rodent neurons, transplant-quality islets, and individual endocrine cells. The combination of nanovial CE-nanoESI-QqQ MS, interface, auto sampler, and fast MRM measurements enables high-throughput, high-sensitivity, and robust metabolite analysis of single cells.Key learning objectives<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Why single cell assays provide key details</li><li style="tab-stops:list 36.0pt;">How to prepare single cells for measurements</li><li style="tab-stops:list 36.0pt;">Why CE/MS works well for single cell metabolomics</li><li style="tab-stops:list 36.0pt;">Benefits of a targeted QQQ approach</li><li style="tab-stops:list 36.0pt;">How to reduce sample volumes</li><li style="tab-stops:list 36.0pt;">On going optimization of CEMS interface</li><li style="tab-stops:list 36.0pt;">Quantify differences in metabolic levels between cells</li><li style="tab-stops:list 36.0pt;">Scientists in the life sciences, with a focus on discovery, or a need for large-scale analyses</li></ul>Who should attend?<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Discovery groups involved with identifying and quantifying biomarkers. Researchers who are sample limited.</li></ul>Session 2:Untargeted metabolomics and lipidomics have traditionally been seen as primarily academic pursuits, often dismissed in clinical research due to perceived challenges in scalability and reproducibility. However, the emergence of precision health, which relies on the concept of digital twins—comprehensive digital representations of the complete molecular content of biological specimens—necessitates the use of untargeted approaches. These methods, in theory, offer the potential to explore an extensive chemical space. But what does it take to implement such analyses on a large scale, aiming both for accuracy in quantification and extensive identification? This presentation will highlight the key lessons learned from the Swiss Personalized Health Initiative.Key learning objectives<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Why is QTOF technology ideally suited for untargeted analyses, both for metabolites and lipidomics</li><li style="tab-stops:list 36.0pt;">What are the critical factors to perform large scale LC-MS (LC, MS, DDA)</li><li style="tab-stops:list 36.0pt;">Harmonization of large-scale data</li></ul>Who should attend?<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">Scientists in the life sciences, with a focus on discovery, or a need for large-scale analyses</li></ul>Certificate of attendance All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.<blockquote>Presenter: Dr. Jonathan V. SweedlerJames R. Eiszner Family Endowed Professor of Chemistry and is affiliated with the Institute of Genomic Biology and the Beckman Institute for Advanced Science and Technology at the University of Illinois Urbana-Champaign, University of Illinois Urbana-ChampaignDr. Sweedler’s research interests focus on developing new lipidomics, metabolomics and peptidomics approaches for assaying small volume samples including new sampling approaches, miniaturized separations, and mass spectrometry. He has used these tools to characterize small molecules and peptides in a range of animal models across metazoan life and in samples as small as individual cells and cellular domains. Sweedler has published more than 500 manuscripts and presented more than 600 invited lectures. &nbsp;He has received numerous awards including the Instrumentation Award from the Analytical Division of the ACS, the Pittsburgh Analytical Chemistry Award, and the ACS Award in Analytical Chemistry. He is a fellow of both the American Association for the Advancement of Science and the American Chemical Society. He is currently the Editor-in-Chief for Analytical Chemistry.Presenter: Prof. Dr. Nicola ZamboniProfessor at the Institute of Molecular Systems Biology of ETH Zurich, and head of the PHRT Clinical Metabolomics Analysis Center., ETH ZurichNicola Zamboni’s research pivots on investigating metabolism and its regulation, from cells to humans. The lab relies heavily on mass spectrometry to quantify levels and rates of conversion of metabolites. In parallel, it develops computational approaches to enable large-scale data acquisition and integration. The lab is embedded in the Swiss personalized health initiatives as main provider of metabolomics and lipidomics for clinical research.Moderator: Matilde Marques (Life Sciences Assistant Editor, SelectScience)</blockquote>

SelectScience: Metabolomics Masterclass: Cutting-Edge Techniques for Single Cell Analysis and Precision Health at Scale

Metabolomics Masterclass: Cutting-Edge Techniques for Single Cell Analysis and Precision Health at Scale

In two sessions, single-cell metabolomics and untargeted metabolomics, and lipidomics will be discussed.

Metabolomics Masterclass: Single Cell Analysis

Session Topics• Important parameters for method development • Method development from scratch or from an experimentInteractive Web SeriesWe’ll work through a topic of the week and discuss any questions that come up along the way. During these sessions, we’ll talk about how to care for your SEC-MALS or FFF-MALS system, help you brush up on your ASTRA and DYNAMICS data processing, discuss measuring dn/dc values and why dn/dc is important, and see how stable RI baselines are achievable. Working with polydisperse samples? We’ll see how to leverage ASTRA’s distribution analysis and results fitting features. Special topics on error analysis, batch MALS work, and more.The live interactive web series are 100% live sessions, with anonymous Q&amp;A welcome during and after the discussion. Free for all Wyatt customers.<blockquote>Presenter: Dr. Christian AckerschottDr. Christian Ackerschott studied Chemistry at the University of Cologne and finished with his diploma thesis in Biochemistry. He completed his studies with the PhD thesis “Characterization of Recombinant Flagelliform Spider Silk Proteins” at the Technische Universität München, where AF4-MALS was used as an essential characterization method.Since 2007, Christian works at Wyatt Technology as Application Scientist and is now Head of Customer Service. His tasks also include customer trainings in Dernbach and onsite as well as education and training of the local Field Application Scientists. &nbsp;</blockquote>

Waters: Method development: How to separate most efficiently?

Method development: How to separate most efficiently?

Working with polydisperse samples? We’ll see how to leverage ASTRA’s distribution analysis and results fitting features.

Join us for an exclusive webinar where we’ll provide an overview of the recently launched Agilent Infinity III LC Series.&nbsp;The session will include a demonstration of the new Agilent InfinityLab Assist technology introduced with the Infinity III LC Series, providing more automation, fewer errors, and increased system uptime in your daily HPLC routine.Don’t miss this chance to stay at the forefront of HPLC technology and engage directly with our experts.Key learning objectives<ul style="list-style-type:disc;"><li style="tab-stops:list 36.0pt;">How to enhance assistance with routines:&nbsp;Explore new features designed to simplify recurring tasks and maximize instrument uptime.</li><li style="tab-stops:list 36.0pt;">How to achieve confidence in results: Discover Agilent’s comprehensive solutions and learn how to leverage 50 years of LC expertise to your advantage.</li><li style="tab-stops:list 36.0pt;">How to protect your investment:&nbsp;Understand how to increase flexibility for maintaining and upgrading your instruments according to your needs and budget.</li><li style="tab-stops:list 36.0pt;">How to make your lab sustainable:&nbsp;Gain insights into reducing your lab’s environmental footprint by reducing water, solvent, and energy consumption.</li></ul>Who should attend?Laboratory managers and LC users in pharmaceuticals, biopharmaceuticals, energy and chemicals, food industry, environmental sciences, and academia who utilize high-performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography (UHPLC) instruments.Certificate of attendance All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.<blockquote>Presenter: Jens Meixner, PhD (Product Manager, Agilent Technologies, Inc.)Jens Meixner is a product manager at Agilent Technologies, where he oversees the analytical core HPLC and CE-MS portfolios. He began his career in 2014 as a PhD student at Aalen University and the University of Tuebingen, where he studied analytical chemistry. During his PhD he focused on the development of multidimensional separation techniques like CExCE and LCxCE coupled with high-end MS detection for the characterization of therapeutic drugs.Presenter:&nbsp;Florian Rieck, PhD (Scientist Application Development, Agilent Technologies, Inc.)Florian Rieck holds a master's degree in food chemistry and a PhD in analytical chemistry from the Karlsruhe Institute of Technology (KIT) in Germany. Florian joined Agilent Technologies in 2015 as an applications chemist for liquid chromatography. An author of numerous application notes covering different analytes and detection techniques, he has also been responsible for a number of customer collaborations and demonstrations of the Agilent InfinityLab LC portfolio.Moderator: Olivia Long (Editorial Team, SelectScience)</blockquote>

SelectScience: HPLC Made for Tomorrow: Introducing the new Agilent Infinity III LC Series

HPLC Made for Tomorrow: Introducing the new Agilent Infinity III LC Series

Join us for an exclusive webinar where we’ll provide an overview of the recently launched Agilent Infinity III LC Series.

Introducing the new Agilent Infinity III LC Series

LC-MS/MS is the gold standard for a wide range of analytical methods in clinical laboratories, with its orthogonality providing powerful levels of selectivity and analytical sensitivity. The Waters Total Solution LC-MS/MS Workflow has been designed to improve the user experience of LC-MS/MS to allow more users to successfully utilize the technique in their laboratories. The workflow can be adopted for a range of small molecules, as well as large molecules, such as the large glycoprotein, thyroglobulin.Using thyroglobulin as an example, we will guide you through aspects of large molecule method development and highlight steps of the workflow that minimize user touch-time, maximize throughput and improve turnaround time. Aspects of the workflow include:<ul><li>Improving the workflow reproducibility with full automation of the Stable Isotope Standards and Capture with Anti-Peptide Antibodies (SISCAPA™) approach using the&nbsp;Andrew+ Pipetting Robot</li><li>Increasing throughput and robustness with Parallel Column Regeneration</li><li>Same day reporting of results using the power of UPLC-MS/MS with waters_connect for IVD and Quan Review</li></ul>Who Should Attend?<ul><li>Laboratory professionals and clinicians who are eager to learn about the benefits of LC-MS/MS in the clinical laboratory workflow</li><li>Laboratory professionals who are seeking to expand their knowledge into using LC-MS/MS for the quantitative analysis of peptides and proteins for clinical research</li></ul><div><div dir=""><div><blockquote><div>Presenter: Dominic Foley (Consulting Scientist, Waters Corporation)</div></blockquote></div></div></div><div><blockquote>Dominic Foley is a Consulting Scientist working at Waters MS Headquarters in Wilmslow, UK. Having joined the company in September 2013, he has specialized in the development of LC-MS/MS clinical research applications, with a focus in endocrinology, particularly steroid hormone analysis. In his role, he also collaborates with clinical customers in the development and implementation of LC-MS/MS methods in their laboratories.Dominic has worked in separation sciences for 17 years, following his ambition of improving the quality of healthcare. He started his career with ICON Development Solutions, a CRO in the north of the UK, where he worked as a LC-MS/MS method development and validation scientist supporting DMPK studies in drug discovery. In 2012, he joined Thermo Fisher Scientific to work in their Chromatography R&amp;D team as a Technical Support Specialist, working on the development of new chromatography and sample preparation products.Dominic received his BSc (Hons) in Biochemistry from the University of Manchester in 2006. He has presented his work at conferences around the world and has publications in journals such as Clinical Chemistry and Laboratory Medicine, Analytical Chemistry and Journal of Chromatography A.</blockquote></div>

Waters Corporation: Quantification of Thyroglobulin in Serum for Clinical Research using SISCAPA Workflow Combined with LC-MS/MS

Quantification of Thyroglobulin in Serum for Clinical Research using SISCAPA Workflow Combined with LC-MS/MS

We will guide you through aspects of large molecule method development and highlight steps of the workflow that minimize user touch-time, maximize throughput.

Quantification of Thyroglobulin in Serum for Clinical

XDCs, such as antibody-drug conjugates (ADC), antibody oligo conjugates (AOC), ribonucleoproteins (RNP), and conjugate vaccines are growing rapidly due to advances in their component technology and their promise of higher efficacy compared to traditional therapies.&nbsp;However, these complex hybrids are more heterogeneous than traditional biologics, posing additional analytical challenges. Quality attributes such as composition, aggregation, purity, and conformation can all affect the efficacy and toxicity of the product.In this online webinar, we will demonstrate the value of light scattering in XDC development and its potential application in quality control (QC) as part of a release assay. We will explore how light scattering provides reproducible and comprehensive quality attribute data in a single platform and provides savings on time and resources for the development of complex XDC drugs.&nbsp;

Waters Corporation: Analysis of PCVs, AOCs, ADCs, and RNPs with Light Scattering

Analysis of PCVs, AOCs, ADCs, and RNPs with Light Scattering

In this online webinar, we will demonstrate the value of light scattering in XDC development and its potential application in QC as part of a release assay

Protein-based therapeutics such as monoclonal antibodies are widely developed and used by the biopharmaceutical industry; in 2023, mAb-based therapeutics represented over half of the Top Ten best-selling drugs of the year, globally, and are one of the fastest growing modalities. However, these large molecules pose unique challenges in relation to sample preparation and analytical characterisation, making robust and fit-for-purpose methods essential to achieve high quality results.This December, we invite you to connect with fellow scientists to learn more about the latest innovations in Protein analysis at our ProteinConnect Virtual Forum. Through a selection of complementary presentations and resources, our specialists will share insights into the present and future of protein characterisation, including:<ul><li>How workflow solutions can address mAb CQA analysis</li><li>Hints and tips for optimising protein bioseparations</li><li>The latest tools and technologies to enhance your protein analysis</li><li>Novel approaches to support online analysis and production of mAbs</li><li>A look to the future – emerging trends in Biopharma analysis</li><li>And much more! agilent ms</li></ul><h5>Agenda 10 December 2024</h5>10:30 a.m. Approaching The Analysis Of mAbsAnd Protein-based Therapeutics: An Introduction11:15 a.m. Optimizing Protein Therapeutic Analysis with Agilent Columns: Innovations And Applications12:15 p.m. Online LC: A Powerful Tool For Process Monitoring During The Production Of mAbs13:00 p.m. ProteoAnalyzer: Analysis of NIST mAbReference Material By Parallel SDS-Capillary Electrophoresis

Agilent Technologies: ProteinConnect: Linking Science, Innovation and Therapeutics

ProteinConnect: Linking Science, Innovation and Therapeutics (Day 1)

We invite you to connect with fellow scientists to learn more about the latest innovations in Protein analysis at our ProteinConnect Virtual Forum.