Microplastics and Nanomaterials: Understanding Emerging Contaminants in Environmental Testing

🎤Matthew BolinoHow do the hidden carbohydrate structures on your favorite protein powders shape the gut microbiome? &nbsp;In this episode of Concentrating on Chromatography, Matthew Bolino, M.S., from the University of Nevada, Reno, breaks down his latest research on N‑glycans from common dietary proteins (whey, egg white, soy, and pea) and how their structural diversity influences microbial fermentation and short‑chain fatty acid production.Bolino explains what N‑glycans are, why they behave like fiber in the gut, and how his team isolates and characterizes them using ethanol washes, enzymatic release (PNGase F and gut‑derived endoglycosidases), and advanced MALDI‑TOF and HILIC‑QTOF workflows. &nbsp;He also discusses his 2025 work comparing synthetic versus bovine whey N‑glycomes and mapping N‑glycan profiles across dietary protein sources, revealing how glycan architecture can reshape community diversity in in vitro fecal fermentations.Geared toward undergraduate and early‑career analytical chemists, this conversation dives into practical mass spec trade‑offs (MALDI vs QTOF vs LC/GC), real‑world troubleshooting in glycomics labs, and how microbiome‑targeted therapeutics and “symbiotic” designs may emerge from pairing specific microbes with preferred glycan structures. &nbsp;Bolino closes with career advice on building biomolecular analysis skills, understanding instrumentation fundamentals, and entering the rapidly growing field of glycomics and microbiome research.<h3>Video Transcription</h3><h4>Introduction</h4>Glycomics has emerged as a rapidly developing field within biomolecular science, expanding our understanding of post-translational modifications beyond well-known processes such as phosphorylation. Among these modifications, protein glycosylation—the covalent attachment of carbohydrate structures to proteins—plays a critical role in biological function, molecular recognition, and cellular communication.This discussion focuses on N-glycans, a class of structurally complex carbohydrates linked to proteins, and their occurrence in common dietary protein sources such as whey, egg white, soy, and pea protein. Particular emphasis is placed on their structural diversity, analytical workflows, and emerging relevance in gut microbiome research.<h4>Fundamentals of N-Glycans</h4>N-glycans are oligosaccharide structures covalently attached to proteins via nitrogen atoms, typically at asparagine residues. They represent one of the two major classes of protein glycosylation, alongside O-glycans.These glycans are characterized by:<ul><li>High structural diversity, arising from variations in monosaccharide composition and linkage patterns</li><li>Large size, typically comprising 7–15 monosaccharide units</li><li>Complex branching architectures, leading to a vast number of possible configurations</li></ul>Common monosaccharide components include:<ul><li>Mannose</li><li>Galactose</li><li>N-acetylglucosamine</li><li>N-acetylgalactosamine</li><li>Sialic acids</li></ul>Unlike free carbohydrates present in foods (e.g., glucose, lactose), N-glycans are protein-bound, which significantly influences their biological behavior.<h4>Biological Function and Dietary Relevance</h4>N-glycans play multiple roles in biological systems, including:<ul><li>Modulation of protein stability</li><li>Regulation of protein trafficking and localization</li><li>Facilitation of cell–cell communication and immune recognition</li></ul>They are particularly abundant on membrane and surface proteins, where they contribute to molecular signaling processes.From a dietary perspective, N-glycans exhibit unique behavior. Due to their size and protein-bound nature, they are not readily digested in the upper gastrointestinal tract. Instead, they can reach the colon, where they may function analogously to dietary fiber, interacting with the gut microbiota.<h4>Research Motivation</h4>The investigation of N-glycans in dietary proteins was initially driven by interest in protein nutrition and supplementation, particularly in the context of muscle growth and protein quality. However, this focus expanded upon recognition of the importance of glycosylation in biological systems.The field of glycomics offers:<ul><li>Extensive structural variability, enabling diverse biological functions</li><li>Relevance in disease mechanisms, including cancer, where glycosylation patterns can influence immune evasion</li><li>Opportunities to explore diet–microbiome interactions</li></ul>This combination of nutritional, biochemical, and biomedical relevance makes N-glycan research particularly compelling.<h4>Experimental Workflow for N-Glycan Analysis</h4>The analysis of N-glycans requires a multi-step workflow due to their covalent attachment to proteins.<h5>1. Protein Purification</h5>Dietary protein samples often contain:<ul><li>Free sugars</li><li>Lipids</li><li>Other matrix components</li></ul>To isolate glycoproteins:<ul><li>Cold ethanol precipitation is used to remove free carbohydrates</li><li>Multiple washing steps ensure removal of residual sugars</li><li>A phenol–sulfuric acid assay is applied to confirm the absence of free sugars</li></ul><h5>2. Enzymatic Release of N-Glycans</h5>N-glycans are enzymatically cleaved from proteins using:<ul><li>PNGase F (Peptide-N-Glycosidase F)</li></ul>This enzyme:<ul><li>Specifically cleaves the glycan from the protein backbone</li><li>Preserves the intact glycan structure</li></ul>Alternative approaches using gut-derived enzymes are being explored to improve accessibility and yield.<h5>3. Glycan Purification and Labeling</h5>Following release:<ul><li>Glycans are purified from the protein matrix</li><li>Chemical labeling is performed (e.g., 2-AA or procainamide tagging)</li></ul>Labeling enhances:<ul><li>Detection sensitivity</li><li>Chromatographic behavior</li></ul><h5>4. Analytical Techniques</h5>Two primary analytical platforms are employed:<ul><li>MALDI-TOF MS<ul><li>High sensitivity</li><li>Broad structural coverage</li></ul></li><li>HILIC-LC-MS<ul><li>Improved reproducibility</li><li>Enhanced chromatographic separation</li></ul></li></ul>These approaches provide semi-quantitative data, where signal intensity reflects relative abundance rather than absolute concentration.<h4>Analytical Challenges</h4>Several challenges are associated with N-glycan analysis:<h5>Low Abundance</h5><ul><li>Dietary proteins typically contain only 1–2% glycan content by weight</li><li>This limits yield and complicates downstream applications</li></ul><h5>Sample Preparation Complexity</h5><ul><li>Extensive purification is required to eliminate free sugars</li><li>Contamination risks must be carefully managed</li></ul><h5>Instrumentation Trade-Offs</h5><ul><li>Different analytical platforms offer complementary advantages</li><li>No single technique provides complete structural and quantitative information</li></ul><h4>Differences Between Plant and Animal N-Glycans</h4>Comparative analysis reveals distinct differences in glycan composition:<h5>Plant-Derived Proteins (e.g., soy, pea)</h5><ul><li>Predominantly high-mannose structures</li><li>Lower monosaccharide diversity</li><li>Simpler glycan architectures</li></ul><h5>Animal-Derived Proteins (e.g., whey, egg white)</h5><ul><li>Greater structural complexity</li><li>Inclusion of diverse monosaccharides (e.g., sialic acids)</li><li>More elaborate branching patterns</li></ul>These differences have important implications for biological function and microbial interactions.<h4>Implications for Gut Microbiome Research</h4>N-glycans represent a potential class of prebiotic substrates that can influence gut microbiota composition and activity.Key findings suggest:<ul><li>Structural diversity of glycans affects microbial utilization patterns</li><li>More complex glycans may promote greater microbiome diversity</li><li>Specific glycan structures may selectively stimulate beneficial microbes</li></ul>This has led to growing interest in:<ul><li>Synbiotic strategies, combining microbes with preferred substrates</li><li>Tailored dietary interventions to enhance metabolic outputs, such as short-chain fatty acids</li></ul><h4>Methodological Considerations</h4>A critical insight from this work is the importance of understanding analytical trade-offs:<ul><li>MALDI-TOF MS provides broader detection of glycan structures</li><li>LC-MS approaches offer greater reproducibility</li></ul>No single technique is universally optimal; instead, method selection should be guided by the specific research question.<h4>Conclusion</h4>N-glycans in dietary proteins represent a complex and biologically significant class of biomolecules with implications spanning nutrition, microbiology, and disease research. Advances in analytical methodologies continue to improve our ability to characterize these structures, despite challenges related to low abundance and structural diversity.A deeper understanding of N-glycan composition and function may enable the development of targeted nutritional strategies and microbiome-based therapeutics, highlighting the growing importance of glycomics in modern life sciences.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

N‑Glycans, Whey Protein, and the Gut Microbiome: Mass Spec Insights with Matthew Bolino

<div><div><div>Our immune system is incredibly powerful: among other things, it can recognize and destroy cancer cells. But tumors can be just as effective at avoiding detection – they build “invisibility shields” to protect themselves from attack.</div></div><div>One breakthrough in cancer therapy has been immune checkpoint inhibitors (ICIs), drugs that reactivate immune T cells and help them fight tumors again. However, only about 20–50% of patients respond well to this treatment. Researchers are therefore seeking alternative therapeutic strategies. A small and well-known molecule called adenosine plays a key role in these efforts.When tumor cells are stressed or dying, they release ATP, which is normally a signal that something is wrong in the tissue. But in the tumor microenvironment, ATP gets rapidly broken down by two enzymes, CD39 and CD73. Released adenosine acts like a chemical “calm-down” signal, binds to immune cells (T cells, NK cells, and others), and tells them to slow down, reducing their ability to kill cancer cells. Targeting CD73 therefore represents a promising strategy for developing new cancer treatments.</div><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UOCHB_Nove_latky_mohou_podporit_imunitni_system_v_boji_s_rakovinou_88c5e82cd5.jpeg" alt="IOCB Prague: Development of potent inhibitors to boost the immune system's ability to fight cancer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_UOCHB_Nove_latky_mohou_podporit_imunitni_system_v_boji_s_rakovinou_88c5e82cd5.jpeg 245w," sizes="100vw" width="661" height="363">At IOCB Prague, interdisciplinary teams led by Jan Řezáč, Jan Konvalinka, and Michal Hocek used a newly developed high-throughput screening method to test over 5,000 compounds, identifying two ribonucleoside derivatives as promising CD73 inhibitors.The scientists then improved the compounds’ activity by carefully modifying the ribonucleoside scaffold with bulky (het)aryl groups. Further optimization improved the pharmacokinetic properties of these compounds, yielding inhibitors with low clearance, long half-life, high solubility, and strong selectivity over related enzymes such as CD39.In cellular experiments, the compounds reduced adenosine production in cancer cells, restored T cell activation, and showed no toxicity toward human fibroblasts. The most active compound showed a single-digit picomolar inhibition constant (Ki).These findings establish a strong foundation for further in vivo efficacy and combination studies, which will explore the therapeutic potential of this novel inhibitor class.<blockquote><h4>The Original article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsptsci.5c00707">Potent Competitive Inhibitors of Ecto-5′-nucleotidase (CD73) based on 6-(Het)aryl-7-deazapurine Ribonucleoside 5′-O-Bisphosphonates</a></h4>Ugnė Šinkevičiu̅tė, Magdalena Šímová, Radek Staník, Lenka Poštová Slavětínská, Kristýna Blažková, Pavel Šácha, Martin Lepšík, Jan Řezáč, Jan Konvalinka, Tereza Ormsby, Michal Tichý, Michal HocekACS Pharmacol. Transl. Sci. 2026, 9, 1, 191–213<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acsptsci.5c00707">https://pubs.acs.org/doi/10.1021/acsptsci.5c00707</a><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>CD73 generates immunosuppressive adenosine in the tumor microenvironment and is a promising target for cancer immunotherapy. We have designed and systematically studied diverse 2-substituted 7-deazapurine ribonucleoside 5′-O-bisphosphonates bearing a variety of (het)aryl groups at position 6 and discovered their highly potent and selective CD73 inhibition activity. The most active compounds (with single-digit picomolar Ki) contained bicyclic (het)aryl groups at position 6 in combination with chlorine at position 2. Further optimization of pharmacokinetic properties identified inhibitors with low clearance, long half-life, high solubility, and excellent selectivity over CD39 and NTPDase3. They effectively suppressed adenosine formation in MDA-MB-231 cells, rescued CD8+ T cell activation, and were nontoxic to human fibroblasts. Overall, their profile compares favorably with AB680, a CD73 inhibitor currently in phase I/II clinical trials.</div>

Development of potent inhibitors to boost the immune system's ability to fight cancer

Article

Video

Scientists

Environment

Scientific article

Interview

Science and research

The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB Prague) is a leading scientific institution in the Czech Republic, recognized internationally. Its primary mission is basic research in the fields of chemical biology and medicinal chemistry, organic and material oriented chemistry, chemistry of natural compounds, biochemistry and molecular biology, physical chemistry, theoretical chemistry, and analytical chemistry. 

Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Agilent Technologies

US HUPO

Technology Networks

Bruker

Thermo Fisher Scientific

SelectScience

SCIEX

Waters Corporation

Diode array detection (DAD) is one of the most used detection techniques for HPLC analysis due to its reliability, ease of use and universal response to chromophores. Newer models continue to increase their sensitivity and resolution. But are you getting the most from your DAD?
In this webinar we will cover the fundamentals of how your DAD works. From there we will discuss the various options for DAD method optimization, from flow cell selection to acquisition method settings. At the end of this webinar, you will walk away with the confidence that you are using your DAD to its fullest capacity.
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Presenter: Melissa Goodlad (Application Engineer, Agilent Technologies, Inc.)
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Melissa Goodlad is an Application Engineer providing applications assistance and technical support for Agilent’s HPLC columns and consumables. Before joining Agilent, Melissa was completing her PhD in Physical Chemistry at UC Merced studying nanoparticle self-assemblies.
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Agilent Technologies: Getting the Most from Your Diode Array Detector: From Selection to Optimization

Getting the Most from Your Diode Array Detector: From Selection to Optimization

we will cover the fundamentals of how your DAD works. From there we will discuss the various options for DAD method optimization.

Diode Array Detector: From Selection to Optimization

<h4>SCIEX OS software summer school</h4>
Master the essentials of SCIEX OS software with our live webinars this summer
Join us for our summer school series dedicated to enhancing your proficiency with SCIEX OS software. Over the course of next months, we offer a series of concise, 30-minute webinars designed to fit into your schedule.
Agenda:
<ul>
<li>23 July, 11:00 a.m. CET – Hardware configuration and instrument verification</li>
<li>6 Aug, 11:00 a.m. CET – Compound optimization</li>
<li>20 Aug, 11:00 a.m. CET – Data processing: quantitation</li>
<li>2 September, 11:00 a.m. CET – Data processing: screening</li>
</ul>

SCIEX OS software summer school - Data processing: screening

Join us for our summer school series dedicated to enhancing your proficiency with SCIEX OS software.

Data processing: screening

Join Dr. Jens Lättig from Tecan and Dr. Cassandra Rusher, European Technical Support Specialist at Eprep, as they explain how the Resolvex® Prep simplifies chromatography and mass spectrometry sample preparation. They will also demonstrate how Resolvex® Prep automates the manual preparation of samples and standards, ensuring consistent performance while minimizing the need for re-validation.
Key learning objectives
<ul>
<li>Learn how the Resolvex® Prep reduces setup time to as little as 5 to 15 minutes, allowing scientists to focus on other critical tasks, while the instrument handles the rest autonomously.</li>
<li>Understand how analytical syringes and specialized tools ensure consistent and reliable results, minimizing the risk of human error in your lab&#39;s processes</li>
<li>Gain insights into achieving reliable, repeatable results across various sample types and workflows</li>
<li>Learn about the versatile capabilities of Resolvex® Prep, including its built-in wash station, RFID deck scan, and seamless sample transfer into analytical instruments, tailored to meet the unique demands of your research</li>
</ul>
Who should attend?
Laboratory scientists and managers from analytical laboratories in: pharma, biopharma, biotech, the food industry, the environmental sector, agriculture, drug screening, proteomics, and metabolomics
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
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Presenter: Jens Lattig, PhD (Sales Development Manager Proteomics &amp; MS Sample Prep, Tecan)
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Dr. Jens Lättig is a Sales Development Manager Proteomics &amp; MS Sample Preparation at Tecan. He has a PhD in biochemistry, and has worked in drug research projects in Germany and France. At Tecan, he is responsible for the Resolvex™ instruments and consumables portfolio in Europe.
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Presenter: Cassandra Rusher, PhD (European Technical Support Specialist, Eprep)
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Dr. Cassandra Rusher is a technical support specialist for the Resolvex Prep. at Eprep. She has a PhD in chemistry and a background specializing in chromatography including sample preparation. Her previous roles have included technical support and product management in the analytical chromatography sector. Her focus now is on supporting the automation of sample preparation methods across a range of industries that are utilizing analytical chromatography techniques.
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Moderator: Carrie Haslam (Associate Editor, SelectScience) 
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SelectScience: Streamline your sample preparation with Resolvex® prep

Streamline your sample preparation with Resolvex® prep

Specialists from Eprep will explain how the Resolvex® Prep simplifies chromatography and mass spectrometry sample preparation.

Sample preparation with Resolvex® prep

Join the MS Imaging SIG for an interactive discussion with authors on experimental design and data pipelines for analysis.
Reference material Nat Methods 20(10):1530-1536. PMCID: PMC10555842
Dr. Rosenberger, and EMBO postdoctoral fellow, will give an educational viewpoint of experimental design ad execution, discussing his results and data analysis in mice.
Caroline Weiss, a PhD student, will discuss transferring automating the developed pipeline for human sample and focus on applications in the clinical context. Dr. Rosenberger and Caroline Weiss work in the laboratory of Matthias Mann at the Max Planck Institute of Biochemistry in Martinsried, Germany.
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Presenter: Dr. Florian Rosenberger (EMBO)
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Presenter: Caroline Weiss (Max Planck Institute of Biochemistry in Martinsried, Germany)
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US HUPO: Spatial Single-Cell Mass Spectrometry in Liver Tissue Slices: Experimental Design and Pipelines for Analysis

Spatial Single-Cell Mass Spectrometry in Liver Tissue Slices: Experimental Design and Pipelines for Analysis

Join the MS Imaging SIG for an interactive discussion with authors on experimental design and data pipelines for analysis.

Spatial Single-Cell Mass Spectrometry in Liver Tissue Slices

Mass spectrometry (MS)-based omics technologies have revolutionized our understanding of molecular changes in organisms and are crucial for studying metabolism and related diseases through lipidomics and metabolomics.
Despite significant advances, there is still a need for robust analytical methodologies in data acquisition, data processing and annotation.
This webinar will demonstrate how LC-Zeno SWATH DIA outperforms conventional DDA in annotating metabolites and lipids in the model organism Gammarus fossarum. Additionally, the presentation will showcase the effectiveness of electron activated dissociation (EAD) in characterizing lipid species and elucidating metabolite isomers. Finally, the webinar will illustrate how EAD and high-resolution targeted approaches improve structural elucidation and molecular network construction.
Attend this webinar to:
<ul>
<li>Learn about the benefits of LC-Zeno SWATH DIA over conventional DDA methods for metabolite and lipid annotation
</li>
<li>Explore the implementation of EAD for enhanced lipid species characterization and metabolite isomer elucidation
</li>
<li>Understand how EAD improves structural elucidation and molecular network construction
</li>
</ul>
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Presenter: Dr. Valentina Calabrese (Postdoctoral Researcher at Institute of Analytical Sciences)
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Valentina Calabrese received her Ph.D. in Analytical and Bioanalytical Sciences at University of Palermo, Italy and is currently a postdoctoral researcher at the Institute of Analytical Sciences in Lyon, France. During her career, she specialized in high resolution, ultra-high resolution, and ion mobility mass spectrometry for application on fundamental topics and -omics research, such as metabolomics and lipidomics. Her research activity focuses on new method development and unknown compound annotation through high-resolution mass spectrometry and molecular networking in biological complex samples. She is currently involved in the study of sentinel freshwater organisms, as Gammarus fossarum and works on DDA and SWATH DIA methods with CID or EAD fragmentation strategies along with molecular networking construction for improving structural characterization of small molecules.
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Technology Networks: Enhancing MS-Based Omics Analysis in a Model Organism

Enhancing MS-Based Omics Analysis in a Model Organism

How LC-Zeno SWATH DIA outperforms conventional DDA in annotating metabolites and lipids in the model organism Gammarus fossarum.

Learn how Light Scattering can benefit your drug development!
A critical step in the development process for an antibody drug conjugate (ADC) is establishing scientifically sound analytical methods that measure key product quality attributes to support pre-clinical activities and downstream clinical release and stability testing. By leveraging key technologies and platform analytical methods, drug developers can expedite an early-phase biologics’ path to IND by removing analytical method development from the critical path.
Join us on an exploration of the analytical characterization required to confidently assess the ID, safety and efficacy of an antibody drug conjugate (ADC). We will start with a practitioners view on the requisite analytics and then shift to several case studies where multiple techniques, from mass spectrometry to light scattering, were used to interrogate these complex protein conjugates.
This webinar promises a comprehensive look at the analytical processes and technologies needed to successfully advance ADC development.
Key learning topics:
<ul>
<li>How to assess Identification, safety and efficacy of ADCs
</li>
<li>The requirements for characterisation are for ADCs
</li>
<li>How multiple techniques (such as mass spectrometry and light scattering) can be used together to understand complex protein conjugates.
</li>
</ul>
 Who should attend?
<ul>
<li>Quality Control and Drug Development Analytical Scientists, Lab Managers and Scientific Directors who are working with ADC characterisation or protein therapeutics in Pharma</li>
</ul>
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Presenter: Eric Miller (Abzena)
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Dr. Eric Miller is an accomplished professional with a strong background in analytical chemistry. He has made significant contributions to the biotech industry, particularly through his work on the LabZient™ Analytical Platform.
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Eric began his journey as a Scientist at ClinMet. He has worked for Agilent Technologies and Pacira Pharmaceuticals before joining Abzena as Senior Director, Scientific Project Leaders, Analytical.
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His expertise lies in addressing the unique needs of biopharmaceuticals.
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Presenter: Colette Quinn, Ph.D. (Sr. Business Development Manager, Waters Corporation)
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Colette Quinn is a Sr. business development manager in the biopharmaceutical group at Waters Corporation. Her focus is around biophysical solutions and how to integrate biophysical and biochemical approaches to enable a “totality of evidence” based approach to problems.
</blockquote>

Waters Corporation: Learning Advanced Analytical Technologies to Expedite the development of Antibody Drug Conjugates

Learning Advanced Analytical Technologies to Expedite the development of Antibody Drug Conjugates

Learn how Light Scattering can benefit your drug development.

Analytical Technologies for Antibody Drug Conjugates

Multiomic and multimodal spatial data from one tissue sample provides actionable information for disease related insights.
Leverage mass spectrometry in the post-genomic era to move beyond the biological determinants of DNA and RNA.Contextualize biological information with proteins and downstream molecules using an innovative benchtop system optimized for ease-of-use.
During this webinar, you will learn:
<ul>
<li>The multiomics capabilities of MALDI Imaging
</li>
<li>The benefits of spatial biology for disease based research
</li>
<li>Ease-of-use of neofleX, a benchtop mass spectrometer for spatial biology
</li>
</ul>
Who should attend:
<ul>
<li>Translational researchers who want advanced spatial biology tools with ease-of-use workflows
</li>
<li>Researchers focused on maximizing spatial multiomics capabilites
</li>
<li>Anyone interested in benchtop mass spectrometers with no compromise on quality register here
</li>
</ul>
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Presenter: Mengzhe Zhang, Ph.D. Candidate, Bernd Bodenmiller Lab, University of Zurich, Zurich, Switzerland
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Presenter: Janina Oetjen, Ph.D., Imaging Applications Scientist, Bruker Daltonics, Bremen, Germany
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Presenter: Kate Stumpo, Ph.D., Senior Market Manager, Bruker Scientific, Billerica, MA, USA
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Bruker:  LabCast: Spatial profiling to transform molecular pathway awareness using neofleX

 LabCast: Spatial profiling to transform molecular pathway awareness using neofleX

The multiomics capabilities of MALDI Imaging.

 LabCast: Spatial profiling to transform molecular pathway

<h4>Rebroadcast of the Live from the Lab: Solutions for Multi-Omics Research from June 13, 2024</h4>
Native nano-DESI mass spectrometry imaging of proteins up to 230 kDa: Advances in proteoform discovery and identification
In this presentation, Prof. Cooper discusses her recent work in developing native ambient mass spectrometry (NAMs) for structural analysis and spatial analysis of proteins.
In this session, you will hear how to
<ul>
<li>identify intact protein assemblies and protein-ligand complexes directly from tissue sections using Native ambient mass spectrometry (NAMs) </li>
<li>understand the structure and function of proteins and their role in complex biological systems </li>
<li>overcome challenges in NAM, including complex mass spectra and low abundance signals Can&#39;t attend the webinar broadcast? No problem! Register anyway and you will automatically receive the link to the recording.</li>
</ul>
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Presenter: Prof. Helen Cooper (University of Birmingham)
</blockquote>

ThermoFisher Scientific: Advances in proteoform discovery and identification

Advances in proteoform discovery and identification

Native nano-DESI mass spectrometry imaging of proteins up to 230 kDa

Are you tired of dealing with inconsistent LC results? Are you looking to extend the life of your LC columns and improve lab efficiency?
Our upcoming webinar, LC Columns Tips &amp; Tricks, is designed to help you get the most out of your LC columns.
Our expert will share invaluable insights on:
<ul>
<li>Understanding LC column chemistry to optimize separations
</li>
<li>Proper handling of new LC columns for maximum lifespan
</li>
<li>Proven techniques to extend column life and reduce downtime
</li>
<li>Effective column maintenance and troubleshooting strategies
</li>
</ul>
This live webinar is an excellent opportunity to deepen your knowledge, improve your lab efficiency, and reduce operational costs. Whether you&#39;re a seasoned pro or just getting started, you&#39;ll walk away with practical knowledge to elevate your column performance and lifetime. Adham Ahmed will be presenting and answering your questions live.
Who Should Attend?
<ul>
<li>Laboratory Analysts and Scientists who are involved in small molecule pharmaceutical analysis
</li>
<li>Chromatographers seeking to optimize LC column performance
</li>
<li>Quality control professionals aiming to improve data reliability and reproducibility
</li>
<li>Research and development scientists working on method development and validation
</li>
<li>Lab Managers: Leaders looking to improve operational efficiency and extend the lifespan of lab equipment.
</li>
<li>Anyone involved in LC column selection, maintenance, and troubleshooting
</li>
</ul>
<blockquote>
Presenter: Adham Ahmed (Global Product Manager, Thermo Fisher Scientific)
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<blockquote>
Adham Ahmed has 15+ years of experience in particle development and functionality for chromatographic application. Presently, he is the Global Product Manager of the Hypersil BDS and ODS and other columns within the Thermo Fisher Scientific Portfolio. His experience includes working in R&amp;D, application specialist and Product management, with a focus on understanding customer’s challenges and laboratory needs.
</blockquote>

Thermo Scientific: LC Columns Tips & Tricks

LC Columns Tips & Tricks

Our upcoming webinar, LC Columns Tips & Tricks, is designed to help you get the most out of your LC columns.

Join this exclusive webinar to discover advanced strategies for enhancing the quality of lentiviral (LV) vectors in cell therapy manufacturing.Quality defects in LV vectors can significantly affect the efficacy and safety of final cell therapies. Despite their critical importance, there has been minimal focus on controlling and monitoring the quality of LV vectors used in cell therapy manufacture. Of particular concern is the risk of ‘breakthrough expression’ of the transgene, leading to CAR expression in producer cells and on the surface of the LV particle.&nbsp;&nbsp;In this webinar, Lentitek will demonstrate how its promoter system is designed to significantly reduce breakthrough expression of the transgene in producer cells, maximizing the functional LV titer during anti-CD19 CAR vector production. Additionally, the webinar will showcase the Leprechaun platform from Unchained Labs, which promises precise identification and monitoring of CAR expression on the LV surface. This webinar is essential for professionals dedicated to improving quality and safety of cell therapies.<blockquote>Presenter: Dr. Alex Shephard (Market Manager, Cell Therapy, Unchained Labs)Presenter:&nbsp;Dr. Adam Inche (Founder &amp; CEO, Lentitek)</blockquote>

Unchained Labs: Preventing the danger of unwanted CAR expression during lentivirus production

Preventing the danger of unwanted CAR expression during lentivirus production

Join this exclusive webinar to discover advanced strategies for enhancing the quality of lentiviral (LV) vectors in cell therapy manufacturing. 