Modernizing Routine HPLC Methods: Aligning Productivity, Compliance, and Sustainability

<h3>How to Easily Adapt Your Existing HPLC Methods</h3>Developing and optimizing an HPLC method is essential for achieving reliable separations, accurate quantification, and reproducible analytical results. As chromatography technology advances, one of the most common optimization strategies is transferring established methods to modern columns with smaller dimensions.Although the benefits are clear, many analysts hesitate when it comes to modifying a validated method.<blockquote>Questions quickly arise: How should the flow rate be adjusted? What injection volume is appropriate? Does the gradient need to be recalculated? Will the retention times change?</blockquote>Even with the necessary equations available, performing all these calculations manually can be time-consuming and prone to errors. Instead of simplifying the workflow, method transfer often becomes an unnecessary obstacle.Fortunately, there is an easier solution.<h4>Simplify Your HPLC Method Transfer</h4>Moving an existing method to a different column does not have to be complicated.The KNAUER HPLC Method Converter is designed to automate the calculations required for method transfer. Simply enter the parameters of your current method, including the original column dimensions, and specify the dimensions of the new column—length, internal diameter, and particle size. The software instantly calculates the recommended flow rate and injection volume for the new conditions.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_7f7c19f7a3.webp" alt="KNAUER: HPLC Method Converter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_7f7c19f7a3.webp 210w," sizes="100vw" width="1022" height="759">The next step is equally straightforward. Copy the original gradient profile into the converter, and the software automatically generates the corresponding gradient program for the new column.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_2_8ba46e78bc.webp" alt="KNAUER: HPLC Method Converter - Gradients" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_2_8ba46e78bc.webp 195w," sizes="100vw" width="1440" height="1150">For even greater confidence, the converter also allows you to enter chromatographic peak information. Based on these data, it predicts the expected retention times under the new conditions. While experimental verification on the HPLC system is still recommended, these predictions provide an excellent starting point for method optimization.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_3_34f16bb3bf.webp" alt="KNAUER: HPLC Method Converter - Chromatograms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_3_34f16bb3bf.webp 196w," sizes="100vw" width="1024" height="814">The converter was developed with one primary objective: making HPLC method adaptation faster, easier, and more reliable while reducing the effort required from the user. Its intuitive interface, modern design, and automatic calculations help save valuable laboratory time—and it is available free of charge.<h4>Modernize Your Methods and Improve Laboratory Efficiency</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_4_31e7188db4.webp" alt="KNAUER: Modernize Your Methods and Improve Laboratory Efficiency" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_4_31e7188db4.webp 245w," sizes="100vw" width="720" height="449"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_5_815a7bf889.webp" alt="KNAUER: HPLC Method Converter - Results" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_5_815a7bf889.webp 194w," sizes="100vw" width="1440" height="1157">One of the major advantages of the KNAUER HPLC Method Converter is its compliance with USP General Chapter &lt;621&gt;.For pharmaceutical laboratories, chromatographic methods must comply with pharmacopeial requirements that ensure product quality and patient safety. Historically, many USP methods were developed using larger HPLC columns that no longer reflect the capabilities of modern chromatography systems.Current USP, European Pharmacopoeia (Ph. Eur.), and Japanese Pharmacopoeia (JP) guidelines now permit specific adjustments to column dimensions and particle size for both isocratic and gradient methods. These changes are allowed provided that defined relationships between chromatographic parameters are maintained.The KNAUER Method Converter automatically checks whether the selected column dimensions remain within the permitted USP limits. If the proposed changes comply with the guidelines, the software confirms that the method can be transferred while remaining pharmacopeia compliant. This removes uncertainty from the method conversion process and helps laboratories modernize their analytical workflows with confidence.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_HPLC_Method_Converter_6_c9d1e28c54.webp" alt="Unsplash: Illustrative picture" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_HPLC_Method_Converter_6_c9d1e28c54.webp 245w," sizes="100vw" width="720" height="449"><h4>Increase Productivity While Reducing Solvent Consumption</h4>Updating HPLC methods is not only about shortening analysis times—it also supports more sustainable laboratory practices.Using shorter columns together with optimized flow rates significantly reduces solvent consumption, lowering operating costs and decreasing the amount of hazardous chemical waste generated during routine analyses. Faster separations also improve instrument throughput, enabling more samples to be processed within the same amount of time.Modernizing chromatographic methods therefore delivers a dual benefit: greater laboratory productivity and a smaller environmental footprint. With only a few simple adjustments, laboratories can improve efficiency while supporting more sustainable analytical workflows.

HPLC method conversion free and easy

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 6th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by Agilent Technologies and Metrohm and posters by Shimadzu/ASMS, Thermo Fisher Scientific/ASMS and Waters Corporation/ASMS!<h3>1. Agilent Technologies: Determination of 41 Per- and Polyfluoroalkyl Substances (PFAS) in Cosmetics</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_captiva_emr_pfas_food_ii_cartridge_5994_9111en_agilent_be91861646.pdf">Full PDF for download</a></li></ul>Per- and polyfluoroalkyl substances (PFAS) are synthetic compounds composed of highly stable carbon–fluorine (C–F) bonds, giving them exceptional thermal and chemical stability, leading to poor environmental degradability. PFAS have been used in cosmetics to provide water resistance, oil repellency, or application smoothness. The unintentionally presented PFAS residues in raw materials may remain in finished cosmetic products, and can be absorbed through the skin. Lip products and spray type products can also lead to inhalation or oral exposure to residues.1,2&nbsp;Agilent Captiva EMR PFAS Food cartridges are designed to provide streamlined and comprehensive matrix removal after QuEChERS extraction for complex sample matrices, demonstrating effective matrix cleanup efficiency and excellent performance for quantitative PFAS analysis.3,4 In this study, we performed QuEChERS extraction followed by cleanup with Captiva EMR PFAS Food II cartridges for PFAS analysis in five types of cosmetics.<h4>Experimental</h4><h5>Equipment and materials&nbsp;</h5>The study was performed using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III">Agilent 1290 Infinity III LC</a> system consisting of an Agilent 1290 Infinity III high-speed pump (G7120A), an Agilent 1290 Infinity III multisampler (G7167B), and an Agilent 1290 Infinity III multicolumn thermostat (G7116B). The LC system was coupled to an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6495D">Agilent 6495D triple quadrupole LC/MS system</a> (G6495D) equipped with an Agilent Jet Stream Electrospray ion source. Agilent MassHunter Workstation software was used for data acquisition and analysis.&nbsp;The 1290 Infinity III LC system was modified using an Agilent InfinityLab PFAS Analysis HPLC conversion kit (part number 5004-0006), including an Agilent InfinityLab PFC delay column, 4.6 × 30 mm (part number 5062-8100). Chromatographic separation was performed using an Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=ZORBAX+RRHD+Eclipse+Plus">ZORBAX RRHD Eclipse Plus</a> C18, 95 Å, 2.1 × 100 mm, 1.8 µm (part number 959758-902).<h4>Results and discussion&nbsp;</h4><h5>Chromatography and calibration curve linearity&nbsp;</h5>Figure 2 shows the overlaid chromatograms for 41 PFAS calibration standards, including 0.05, 0.1, 0.2, 0.5, 1.0, 2.0, 5.0, and 10.0 ng/mL in ACN. The table on the right shows the peaks identification and corresponding retention time. Across 41 PFAS compounds, R² values ranged from 0.9940 to 0.9998, indicating excellent linearity for all analytes' calibration curves across the calibration range of 0.05 to 10.0 ng/mL.<h5>PFAS detection in real cosmetic samples&nbsp;</h5>Of the 15 cosmetic products assessed, one eyeshadow sample was detected with a trace level of positive PFAS analytes, including PFHeA, PFHpA, PFNA, and PFOA, at about 0.1 ng/g in the sample. Figure 5 shows the method blanks and positive detection of PFHpA and PFNA in this eyeshadow&nbsp;product.<h4>Conclusion&nbsp;</h4>A simplified, rapid, and reliable method using QuEChERS extraction followed by EMR mixed-mode passthrough cleanup using the Agilent Captiva EMR PFAS Food II cartridge and LC/MS/MS detection was developed for 41 PFAS analysis in cosmetics.<h3>2. Metrohm: brightRC – Advanced brightener analysis with a response curve</h3>Next-level brightener determination in nonlinear systems<ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_v_242_download_83376b2923.pdf">Full PDF for download</a></li></ul><h4>WHAT IS BRIGHTRC?&nbsp;</h4>brightRC is a response curve calibration approach for the determination of brightener-type additives by CVS or CPVS (Cyclic Pulse Voltammetric Stripping), hence the name «brightRC». This technique is suitable to measure brightener content in copper baths for plating. The method is based on signal normalization using the electrolyte value, which is the response for the virgin makeup solution (VMS) saturated with suppressor, and brightener defined as Q0 in the viva software. All recorded signals are divided by Q0 and the normalized values are plotted against concentration to generate an external calibration curve. This calibration can then be used for routine sample determination without repeated standard additions.&nbsp;The sample is simply measured using the same method as the calibration, signals are normalized and evaluated using the mathematically fitted calibration curve. By decoupling calibration from sample measurement, brightRC removes the need for repeated standard additions with each sample measurement, enabling faster and more efficient routine analysis.&nbsp;The brightRC calibration approach supports flexible regression models including quadratic and nonlinear regression. This allows reliable quantification of additive systems that do not exhibit a wide linear working range or exhibit nonlinear response characteristics, thereby expanding the range of applicable brightener chemistries.&nbsp;One important detail that further differentiates brightRC from the MLAT approach relates to normalization of the signal for calibration and sample analysis. This normalization approach improves measurement robustness as variations in absolute signal intensity naturally occurring over the working day are mathematically compensated.<h4>HOW DOES BRIGHTRC WORK IN PRACTICE?&nbsp;</h4>The brightRC workflow separates calibration and sample analysis. It can be implemented in different setups (from manual to fully automated) depending on the additive system and concentration range. After recording the electrolyte value, calibration standards are measured to establish the calibration curve, otherwise known as the response curve. Once the calibration is stored, samples are determined with the same CVS or CPVS method, without further standard additions. The sample concentration is automatically calculated from the normalized signal using the calibration curve.&nbsp;For sample measurement, viva 4.0 provides two practical approaches:&nbsp;<ol><li>«brightRC with solution exchange»&nbsp;</li><li>«brightRC with sample dilution» Both variants follow the same calibration principle and differ only in the sample handling strategy.</li></ol><h4>WHEN TO USE BRIGHTRC?&nbsp;</h4>brightRC is ideal for routine brightener control in stable plating baths where high sample throughput is required. It is particularly suitable when matrix conditions are consistent and electrode fouling is limited. The approach is especially advantageous for additive systems exhibiting nonlinear signal behavior. If strong matrix variations, significant breakdown products, or severe electrode fouling are expected and a linear correlation between signal and analyte concentration is guaranteed, standard addition techniques such as MLAT are preferred because they may provide additional robustness.<h3>3. Shimadzu / ASMS: PFAS Analysis of Leachate: Evaluating Key Sample Preparation and LC–MS/MS Parameters for Improved Performance</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/WP_255_PFAS_Analysis_of_Leachate_Evaluating_Key_Sample_Preparation_and_LC_MSMS_Parameters_for_Improved_Performance_1114ee264c.pdf">Full PDF for download</a></li></ul>Landfill leachate is a highly complex and variable matrix that poses significant challenges for the analysis of per- and polyfluoroalkyl substances (PFAS). Although U.S. Environmental Protection Agency Method 1633A provides a standardized framework for PFAS quantitation,1 analytical performance remains strongly influenced by sample preparation, chromatographic separation, and mass spectrometric conditions. Landfill leachate samples were extracted using WAX SPE following EPA 1633A and analyzed by LC–MS/MS using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8060">Shimadzu LCMS-8060</a> under negative ESI conditions. This study evaluates the key factors governing LC–MS/MS sensitivity, signal response, and reproducibility in landfill leachate, including a systematic assessment of SPE, chromatographic behavior, and ionsource conditions across PFAS classes (Figure 1).<h4>Results&nbsp;</h4>Chromatographic performance was evaluated across four C18 columns (A–D) using peak area, height, asymmetry, and HETP. Two columns produced a higher signal and improved peak shape, while the remaining columns showed greater efficiency. Under repeated batches followed by storage in acetonitrile, the Shimadzu GIST column produced a consistent signal (≤15% change), whereas another column showed 20–50% signal loss, particularly for long-chain PFAS and precursors, indicating greater susceptibility to matrix accumulation (Figure 2).<h4>Conclusion&nbsp;</h4><ul><li>PFAS analytical performance was dependent on compoundclass-dependent extraction, chromatography, and ionization behavior.&nbsp;</li><li>GIST demonstrated lower signal variability under repeated matrix exposure, with lower signal degradation than alternative C18 phases.</li><li>Neutral PFAS showed poor retention on WAX sorbents, requiring optimization beyond standard EPA 1633A extraction conditions.&nbsp;</li><li>PFCA response was more influenced by gas flow conditions, whereas PFSA response was more sensitive to source temperature conditions.</li></ul><h3>4. Thermo Fisher Scientific / ASMS: Ultra-high throughput proteomics at &gt;500 samples/day using advanced <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Vanquish Neo UHPLC</a> methods with <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959">Orbitrap Astral Zoom MS</a></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004708_lsms_ultra_high_throughput_vanquish_astral_proteomics_po004708_asms2026_na_en_f6499c3eec.pdf">Full PDF for download</a></li></ul>High-throughput mass spectrometry is increasingly critical for large-cohort proteomics, population-scale studies, and screening applications. While established LC–MS platforms routinely support several hundred samples per day, further increases in throughput are often limited by chromatographic stability, robustness, and reproducibility under ultra-short gradient conditions, as well as the acquisition speed of the mass spectrometer. Here, we describe an advanced ultra-high-throughput LC–MS methodology developed on the Thermo Scientific Vanquish Neo UHPLC system and Thermo Scientific Orbitrap Astral Zoom mass spectrometer. The goal of this work was to extend the practical throughput limits of LC–MS while achieving deep proteome coverage and maintaining run-to-run reproducibility.<h4>Materials and methods</h4><h5>LC-MS analysis&nbsp;</h5>Samples were separated on a Thermo Scientific 150 µm × 70 mm C18 column (prototype, not commercially available yet) operated at 4 µL/min using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398">Vanquish Neo UHPLC system</a> in NanoCap Trap&amp;Elute configuration at room temperature. Prototype methods allowing for reduced LC duty cycles (approx. 1 min of sample pickup/loading and integrated trap-column offline wash) were applied, the standard gradients were adjusted to 1 – 3 min. The Thermo Scientific PepMap Neo 5 µm C18 300 µm × 5 mm cartridge was used as trap column and the separation column connected with a 15 µm ID capillary Thermo Scientific EASY-Spray emitter (ES994) to an EASY-Spray source. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959">Orbitrap Astral Zoom MS</a> (ICSW AST 2.2) was operated in a dataindependent acquisition (DIA) mode, MS method parameters are given in Table 1. Comparative data for the chemoproteomics samples at lower throughputs (60-300 SPD) were acquired using an EASY-Spray 150 µm × 150 mm C18 column (ES906), as described before.&nbsp;<h5>Data analysis&nbsp;</h5>The raw data files were processed with Spectronaut® 20.5 software (Biognosys AG), <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782">Thermo Scientific Proteome Discoverer </a>3.3 SP1 with CHIMERYS 5.0 or DIA-NN Enterprise 2.3.2 (Aptila Biotech GmbH) nodes using default settings as specified in the individual figures. The data was searched against the Homo sapiens SwissProt database (TaxID=9606: 42,252 sequences) and contaminants FASTA file. Peptide numbers correspond to unique stripped peptides<h4>Conclusions&nbsp;</h4><ul><li>The advanced Vanquish Neo UHPLC methodology in combination with the Orbitrap Astral Zoom MS delivered highly reproducible, deep and precise proteome profiling across ultra-high-throughput regimes (600 SPD) exceeding current field benchmarks.&nbsp;</li><li>The workflow enables rapid chemoproteomic screening, as demonstrated with activity-based protein profiling (ABPP) samples, reliably identifying enriched putative target proteins of an antibiotic small molecule probe.</li></ul><h3>5. Waters Corporation / ASMS: Assessing Relative Response of Four European-Regulated PFAS in Human Serum Using Cyclic Ion Mobility MS</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009410_c98e7c87ec.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster presents an LC-Cyclic-IMS-MS approach for assessing four European-regulated PFAS—PFOS, PFOA, PFNA, and PFHxS—in human serum. The study focuses on environmental exposure monitoring in Ghanaian firefighters and e-waste handlers, using ion mobility mass spectrometry to add separation power and improve identification confidence in complex biological matrices. The work is motivated by increasing regulatory concern around PFAS toxicity, bioaccumulation, and the need for reliable exposure assessment in human biofluids.The experimental workflow combined reversed-phase LC with a quadrupole-cyclic ion mobility-time-of-flight MS system (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/202">SELECT SERIES Cyclic IMS</a>). Human serum samples were prepared using SPE µElution plates with a polymeric reversed-phase/weak anion exchange sorbent. The LC system was modified with PFAS-free components and an isolator column to reduce background contamination, which is especially important for trace-level PFAS analysis. Identification was supported by retention time, accurate mass, isotopically labelled internal standards, and collision cross section values.The results showed detectable levels of the four regulated PFAS in both occupational groups, with a combined detection rate of 83% across human serum samples and NIST reference standards. Firefighter samples generally showed higher concentration ranges than e-waste handler samples, especially for PFOS, PFOA, PFNA, and PFHxS. For example, PFNA reached up to 5.713 ng/mL in firefighter serum, compared with up to 1.513 ng/mL in e-waste handler serum.Overall, the poster concludes that LC-Cyclic-IMS-MS provides a highly specific strategy for PFAS analysis in complex biological samples. Ion mobility and CCS values improve confidence in trace-level identification and help distinguish target PFAS from co-eluting matrix background. The authors suggest that this approach can support both targeted PFAS monitoring and broader non-targeted screening of known and emerging PFAS, while also helping future studies connect exposure data with environmental fate and potential toxicological effects.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 28, 2026

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A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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When developing an LC method, there are various factors that can be tested to achieve the desired resolution and symmetry of the analytes.In this webinar we will discuss the various factors and give tips on developing a robust method.<blockquote>Presenter: Laura Montis (Product Specialist Liquid Phase Separations, Agilent)Laura began her carrier in the Liquid Chromatography over 15 years ago. After experiencing the pharmaceutical world in an analytical development lab, she went on to work with preparative chromatography and gained experience with Supercritical Fluid Chromatography prior to moving onto special HPLC applications, like 2DLC and Online LC.Throughout her career, Laura has held several positions, including application chemist, trainer, sales specialist, and marketing business development. She is currently Product Specialist for liquid phase chromatography systems within the International Distributor Organization in EMEA at Agilent Technologies.Laura studied analytical chemistry and holds a master’s degree from University of Lyon in France.</blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Method Development in HPLC

In this webinar we will discuss the various factors and give tips on developing a robust HPLC method.

Join our upcoming webinar on the MassHunter Quant Screener Tool to discover how to harness the power of quantitative analysis for effective screening and identification of potential suspect compounds.This session will provide in-depth guidance on creating methods from PCDL databases, evaluating result parameters, and generating straightforward summary reports. Additionally, learn how retention time (RT) prediction can enhance your screening process by significantly accelerating and simplifying large database searches.Don’t miss this opportunity to elevate your analytical capabilities!<blockquote>Presenter: &nbsp;Jonathan Spencer (Application Specialist, Agilent Technologies)Jonathan studied BSc Natural Sciences at the University of Nottingham and later went on to Sheffield Hallam University to study a MSc in Forensic Science.Following graduation, he spent 2 years as a Confirmatory Analyst in an anti-doping and forensic toxicology lab, before joining Agilent as an LC/MS Applications Scientist. At Agilent, he focuses on small molecule applications using LC/TQ and LC/Q-TOF in fields of like food, forensic and environmental analysis.</blockquote>

Agilent Technologies: MassHunter Masterclass Months

MassHunter Quant Screener Tool

Discover how to harness the power of quantitative analysis for effective screening and identification of potential suspect compounds. 

In this presentation, we will explore groundbreaking advancements in the bioanalysis of antisense oligonucleotides (ASO), achieving remarkable sub ng/mL limits of quantitation (LOQ). We’ll dive into practical and scalable solutions that ensure precise quantitation of these complex molecules.Discover innovative sample preparation techniques designed to maximize recovery, enhance repeatability, and boost LC-MS sensitivity. Additionally, we’ll showcase the latest LC-MS hardware from Waters, which not only delivers industry-leading negative ionization efficiency, but also saves valuable bench space and reduces utility usage compared to competitors.<h5>Key Learning Objectives:&nbsp;</h5><ul><li>Explore scalable solutions for quantifying complex oligonucleotides across a wide range of concentrations.</li><li>Discover practical approaches that enhance recovery, repeatability, and LC‑MS sensitivity.</li><li>Take advantage of the opportunity to attend the presentation live, where you'll have the chance to ask questions, engage, clarify, and deepen your understanding of the latest innovations in bioanalysis.</li></ul><div><blockquote>Presenter: Andrew Leightner (Senior Principal Market Development Manager, Pharmaceuticals and Biopharmaceuticals, Waters Corporation)<div>Andy Leightner has over 10 years of experience serving the pharma market from contract research organizations. He specialized in method development and validation leveraging LC, GC, and mass spectrometry to meet complex client needs. He supports pharmaceutical and biopharmaceutical discovery and development in the Americas for Waters Corporation. Andy received a bachelors degree in biology from the University of Wisconsin-Madison.</div></blockquote></div>

Elevating Oligonucleotide Bioanalysis: LOQ, Dynamic Range, and Innovative Sample Preparation Techniques

We will explore groundbreaking advancements in the bioanalysis of antisense oligonucleotides (ASO), achieving remarkable sub ng/mL limits of quantitation (LOQ).

Oligonucleotide Bioanalysis: LOQ, Dynamic Range, SamplePrep

Key Learning Points:<ul><li>Gain a comprehensive understanding of solvent miscibility and recognize its significance</li><li>Develop an in-depth comprehension of how to properly solvent switch a column, and the potential consequences associated with incorrect procedures</li><li>Familiarize yourself with a standardized protocol for transitioning an LC column from one solvent system to another, ensuring a seamless solvent switch</li></ul>Who Should Attend:&nbsp;Any LC users who are looking to understand the factors that can impact a systems backpressure and how to mitigate potential backpressure problems.<blockquote>Presenter: Koen Askamp (Technical Specialist, Phenomenex)I am from the Netherlands, studied Molecular Life Sciences at Wageningen University (Netherlands), majoring in immunology. Analytical Chemistry was one of my favorite courses – who doesn’t love solving puzzles?I like drumming, scuba diving, motorcycle-riding, and this past winter I learned how to ski (a little)! &nbsp;</blockquote>

LC Pressure 101

Familiarize yourself with a standardized protocol for transitioning an LC column from one solvent system to another, ensuring a seamless solvent switch.

<div><div dir="" aria-atomic="true">Quality control (QC) labs today require instruments that can accurately and reproducibly tackle a broad range of applications in a way that is reliable and compliance ready.Join us for an interactive educational session where we explore how the expansion of liquid chromatography (LC) detection can significantly enhance the capabilities and possibilities in QC analyses. See how the addition of PDA detection in an integrated, next-generation HPLC system, elevates QC labs to achieve:<ul><li>Enhanced analytical capabilities: Discover how the use of columns with smaller particle sizes facilitates seamless method adjustment or scaling on the Alliance iS HPLC System.</li><li>Wide application range: Suitable for a broad range of compounds due to its versatile wavelength range.</li><li>Method migration support: Tools to support moving methods from other HPLC systems, ensuring comparable results.</li><li>Data integrity: Provides reliable and reproducible results crucial for regulatory compliance and quality control.</li></ul>Introducing the Alliance iS HPLC SystemThe Alliance iS HPLC system is the first-of-its-kind LC system that brings intuitive simplicity and performance to routine analyses. Initially impressing pharmaceutical QC lab teams with its integrated TUV detector, it has evolved to serve QC biopharma labs with the Alliance iS Bio HPLC System, featuring MaxPeak High Performance Surfaces (HPS) Technology.Now, with the latest addition of the integrated PDA detector, the Alliance iS HPLC System enters a whole new arena of applications.Next-Level UV Analysis for QC Labs<ul><li>3D spectral data acquisition wavelength: Capable of recording multiple 2D channels and 3D spectrum.</li><li>Versatile PDA detector: Applicable to any UV-based HPLC method with an wavelength range of 190-800 nm, allowing for the exploration and tracking of multiple peaks of interest with various lambda max.</li><li>Peak purity analysis: Conduct peak purity analysis through Empower Chromatography Data System (CDS).</li><li>Peak identification and confirmation: Identify or confirm peaks by individual spectrum, a key step in assay, impurity, and ID pharmaceutical methods.</li></ul>Case Studies<ul><li>Scaling a method from the Alliance iS HPLC System with PDA Detector: Learn how to scale an HPLC method to 2.5 um particle column on the Alliance iS HPLC System with PDA detector, taking advantage of pressure range for shorter run times and lower solvent consumption.</li><li>Method migration of a USP monograph to the Alliance iS HPLC System with PDA Detector: Understand impact of instrument characteristics on method migration or transfer, and how to successfully move your method from different vendor systems to the Alliance iS HPLC System with PDA Detector.</li></ul>Don't miss this opportunity to learn how to leverage the latest advancements in LC detection to enhance your QC lab's capabilities and efficiency.<blockquote>Presenter: Patrick Flanagan (Principal Product Marketing Manager, QA/QC, Waters Corporation)</blockquote><div><blockquote>Patrick Flanagan is a Principal Product Marketing Manager in Waters’ QA/QC Division, where he has worked for over six years. He currently is responsible for product marketing and product management activities for Waters’ next generation HPLC systems.He received his bachelor’s in science degree from Worcester Polytechnic Institute in Biochemistry and worked for 15 years as a formulation scientist.Patrick began his career in Waters’ product management department responsible for optical detection, multi-dimensional systems, the Alliance HPLC System, Arc Premier System, Arc HPLC System, and the new Alliance iS HPLC System. His recent work has been focused on the development of innovative new products for pharmaceutical QA/QC departments.</blockquote><blockquote>Presenter: Lise Gauthier (Principal Evaluation Scientist, Waters Corporation)Lise Gauthier is Principal Scientist within the QA/QC Business Strategy group at Waters Corporation in Milford, MA. Prior to joining Waters in 2014, Lise worked in the pharmaceutical industry holding within Quality Assurance and Drug Discovery. Her current role at Waters includes system validation and evaluation of competitive systemsPresenter: Amanda Dlugasch (Senior Scientist, Waters Corporation)Amanda Dlugasch is a Senior Scientist in the QA/QC Strategy and Growth Department at Waters Corporation in Milford, MA. She received her B.A. in Chemistry from Manhattanville College and her M.S. in Chemistry from Sacred Heart University. She started at Waters Corporation in the Instrument Evaluation group developing qualification protocols for newly released products. Upon transferring to the QA/QC Strategy and Growth Department, her initial focus was to evaluate and provide application and training material for the release of ACQUITY Arc UHPLC System. She studied how LC instrument design and LC system characteristics influence chromatography when transferring, scaling, and modernizing methods. Currently, she is working on evaluating the Alliance iS HPLC System.</blockquote></div></div></div>

Waters: Expanding Capabilities in QC Analyses with Advanced LC Detection

Expanding Capabilities in QC Analyses with Advanced LC Detection

Explore how the expansion of liquid chromatography (LC) detection can significantly enhance the capabilities and possibilities in QC analyses. 

Analyses with Advanced LC Detection

<div id="zephr-body-wall"><div>Recent technological and computational advances in spatial transcriptomics have increased interest in complementary techniques for characterizing the multi-dimensional architecture of tissues, such as using mass spectrometry imaging (MSI) to map the distribution of metabolites and lipids.Unlike liquid chromatography/mass spectrometry (LC/MS), where metabolites and lipids are usually measured from whole samples in bulk, MSI uses a scanning probe to collect spatially resolved data across a tissue section. Although its chemical coverage is typically inferior to LC/MS, MSI can still measure metabolites and lipids with high sensitivity and molecular specificity.Dr. <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/articles?search=Gary+Patti">Gary Patti</a>, Professor of Chemistry, Genetics and Medicine, Washington University, will provide an overview of the various technologies available for MSI, covering different sources and mass spectrometers, from home-built solutions to commercial options. The pros and cons of each approach will be briefly discussed, and their application to imaging metabolic fluxes will be explored.Key learning objectives<ul><li>Understand the advantages and disadvantages of MSI compared to LC/MS analyses</li><li>Explore the best way to establish metabolite identifications when performing MSI</li><li>Discover the benefits of using triple quadrupole instruments for MSI</li><li>Consider the benefits of coupling MSI with isotope labelling</li></ul>Who should attend?<ul><li>This event is intended for anyone with an interest in mass spectrometry imaging or spatial biology. Extensive expertise in mass spectrometry is not required.</li></ul>Certificate of attendance All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.<blockquote>Presenter: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/articles?search=Gary+Patti">Gary Patti, Ph.D.</a> (Professor of Chemistry and of Genetics and Medicine, Washington University)Moderator: Matilde Marques (Life Sciences Assistant Editor, SelectScience)</blockquote></div></div>

SelectScience: Metabolite imaging signals the next frontier in spatial biology

Metabolite imaging signals the next frontier in spatial biology

An overview of the various technologies available for MSI, covering different sources and mass spectrometers, from home-built solutions to commercial options.

Metabolite imaging - the next frontier in spatial biology

As CHO-based processes for recombinant proteins continue to have increased cell densities and titers, the need for advancement in clarification technologies is of importance to enable manufacturing scalability. A novel technology, anion exchange (AEX) fiber chromatography, aims to address this challenge and has proven to be scalable up to large commercial manufacturing. This approach combines the ease of filter-like flowthrough operation and scalability, as well as the separation precision of chromatography.Join Dr. Masa Nakamura, Senior Application Development Specialist, Solventum, as he showcases a new clarification platform strategy that can offer seamless implementation and deployment from discovery to clinical and commercial manufacturing. It can also provide consistent and high-quality clarified fluid to enhance commercialization productivity.Key learning objectives<ul><li>Learn about the application of synthetic fiber chromatography to the clarification unit operation</li><li>Explore a novel approach to harvest and clarification in CHO processes</li><li>Understand options to overcome scale up challenges and to reduce soluble impurities during clarification</li></ul>Who should attend?Scientists, Engineers, and Heads of process development, manufacturing, MSAT, and CMC from biopharmaceutical organizations focused on recombinant protein production in CHO expression systems.Certificate of attendanceAll webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes<blockquote>Presenter: Masa Nakamura, PhD (Senior Application Development Specialist, Solventum, Bioprocessing Filtration)</blockquote><div><blockquote>Dr. Masa Nakamura, Senior Application Development Specialist for Solventum, is responsible for developing new products and applications for biopharmaceutical clarification and purification processes, including the development of fiber chromatography products. He has over 25 years of bioseparation and bioengineering experience in academic and industry. He received his BS in Chemical Engineering and PhD in Biochemical Engineering from the University of Tokyo, Japan.Moderator: Matilde Marques (Life Sciences Assistant Editor, SelectScience)</blockquote></div>

SelectScience: Scalable chromatographic clarification platform for mAb manufacturing in CHO systems 

Scalable chromatographic clarification platform for mAb manufacturing in CHO systems 

A new clarification platform strategy that can offer seamless implementation and deployment from discovery to clinical and commercial manufacturing.

Chromatographic platform for mAb manufacturing in CHO

Are you ready to transform your pharmaceutical operations? Don’t miss our exclusive session on Shimadzu Digital Classrooms and begin your journey towards Pharma digital transformation (DX). Discover how Shimadzu’s pioneering AI technologies and digital solutions are ushering in a new era of pharma innovation.In this informative session, explore how Shimadzu is reshaping the drug development landscape. Learn how our innovative tools effectively streamline workflows, enhance lab efficiency, and maintain strict compliance with regulatory standards.Whether you’re a forward-thinking researcher, a quality assurance professional, or a strategic decision-maker, this session is your gateway to mastering AI and digital transformation. Equip yourself with the expertise and resources to stay ahead in the industry.This is an opportunity not to be missed – Register Now!Learning Objectives:&nbsp;<ul><li>Understand how to stay conforming to the Pharma regulations and USP 621 changes with Shimadzu Living Laboratory</li><li>Explore Shimadzu AI and Automation to elevate your lab workflows effectively</li><li>Learn about the AI features of Shimadzu Ai-series and Nexera series</li><li>Gain insights into efficient quality control strategies with Shimadzu Data Management and Software</li></ul><blockquote>Presenter: Liw Wan Tung (Assistant Market Manager (Pharma) Shimadzu (Asia Pacific) Pte Ltd)Wan Tung is the LC Product Manager and Pharmaceutical market in-charge at Shimadzu Asia Pacific Pte Ltd. She is the LC expert with more than 15 years of working experience in analytical science, specializing in method development of various LC and LCMS applications. In 2012, she joined Shimadzu to develop end-to-end workflow solutions for the customers. She has supported a multitude of applications from impurity analysis to drug discovery and biopharmaceutical applications. Prior to joining Shimadzu, Wan Tung started her professional career as a QAQC chemist in Pharmaceutical multinational company. Wan Tung earned her bachelor’s honour's degree in chemistry back in 2009.Presenter: Kanimozhi Sivarajan (Informatics Manager Shimadzu (Asia Pacific) Pte Ltd)Dr. Kani is having 10 years of experince in Laboratory Management and Informatics &nbsp;Systems. She is serving as Informatics Manager at Shimadzu Asia Pacific, Singapore, &nbsp;providing Lab Informatics Solutions such as LabSolutions CS, LIMS and other QMS applications. She is supporting customers from various segments on implementing laboratory automation and responsible for providing consultation on streamlining Lab Workflows, Regulatory Compliance requirements for Electronic Data Management and Lab Practices/Analysis.&nbsp;</blockquote>

Shimadzu:  Realizing Pharma DX: Discover Shimadzu’s AI-Driven Solutions And Automation

 Realizing Pharma DX: Discover Shimadzu’s AI-Driven Solutions And Automation

Discover how Shimadzu’s pioneering AI technologies and digital solutions are ushering in a new era of pharma innovation.

Pharma DX: Shimadzu’s AI-Driven Solutions And Automation

Did you know pipetting errors can quickly amplify across a normal workflow? And as protocols become more complex, there is an increased risk for experimental errors. Experimental error can propagate from many different sources:&nbsp;<ul><li>Incorrect calculations during experimental planning</li><li>Unstable or incorrect sample or reagent preparation</li><li>Incorrect instrument use or pipetting technique</li></ul>Join our live webinar to learn strategies for reducing pipetting errors to help you produce consistent and reliable results.Webinar agenda:<ul><li>Pipetting error propagation</li><li>Good science is control</li><li>Quick checking a pipette</li><li>Pipette performance verification</li><li>Pipette management software</li></ul>

Mettler-Toledo: Strategies for Reducing Pipetting Errors 

Strategies for Reducing Pipetting Errors

Join our live webinar to learn strategies for reducing pipetting errors to help you produce consistent and reliable results.

Strategies for Reducing Pipetting Errors 

This presentation discusses the work being done to understand how drugs work and how they can be repurposed for new indications. Prof. Küster’s lab uses various technologies and methods, such as sample preparation, mass spectrometry, and informatics, to study the proteome and fosso-proteome of cancer patients.The goal is to find the right drug for the right person by understanding what is going wrong in the patient and what the drugs can do. By studying drug-protein interactions and pathway engagement in a dose-dependent fashion, this research aims to make better treatment recommendations. The lab has developed a technology platform that allows for the systematic profiling of kinase inhibitors and the measurement of proteome expression changes in response to drugs.This information can help prioritize biological effects and facilitate drug repurposing. This presentation highlights the importance of studying proteins in understanding drug mechanisms of action and identifying potential targets for cancer treatment.Learning points:<ul><li>Importance of Repurposing Drugs &nbsp;</li><li>Role of Proteomics in Precision Oncology &nbsp;</li><li>Dose-Dependent Analysis &nbsp;</li></ul><blockquote>Presenter: Prof. Bernhard Küster (Technical University Munich)Bernhard Küster holds a chemistry degree from Cologne, a PhD in Biochemistry from Oxford and did a PostDoc at the EMBL in Heidelberg and the University of Southern Denmark. After seven years as Vice President Analytical Sciences and Informatics at Cellzome (now GSK), he became.​Full Professor of Proteomics at the TU Munich in 2007. He also directs the Bavarian Biomolecular Mass Spec-trometry Center and the Clinical Mass Spectrometry Center Munich.​Bernhard’s research focuses on proteomics and its application to chemical and cancer biology. He is interested in understanding how cancer drugs actually work and has received an ERC Advanced Grant in 2019 aiming to integrate proteomics into precision medicine. Bernhard has received several awards, including from HUPO for a draft human proteome and his contri-butions to chemical proteomics. Bernhard is a member of the German National Academy of Science, Leopoldina and has co-founded two start-up companies that operate in the area of proteomics and artificial intelligence.</blockquote>

Thermo Fisher Scientific: Understanding drugs using proteomics

Understanding drugs using proteomics

This presentation highlights the importance of studying proteins to understand drug mechanisms and identify potential targets for cancer treatment

Strategies for Reducing Pipetting Errors

LC Pressure 101

Metabolite imaging signals the next frontier in spatial biology

Expanding Capabilities in QC Analyses with Advanced LC Detection

Elevating Oligonucleotide Bioanalysis: LOQ, Dynamic Range, and Innovative Sample Preparation Techniques

MassHunter Quant Screener Tool

Understanding drugs using proteomics

Method Development in HPLC

Realizing Pharma DX: Discover Shimadzu’s AI-Driven Solutions And Automation

Scalable chromatographic clarification platform for mAb manufacturing in CHO systems

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