<p><strong>Researchers</strong><span><strong>&nbsp;</strong></span><strong>Tereza Nešporová and Jaroslava Večerková from Brno's </strong><span><strong>RECETOX</strong></span><strong> explain why failures are also a valuable part of gaining knowledge, how they study the effects of chemical substances on the hormonal system, and why they apply their professional insights to their everyday lives.</strong></p><h5><strong>How did you get involved in research at RECETOX?&nbsp;</strong></h5><p><span><strong>Jaroslava:</strong></span> <span>I actually joined RECETOX by chance during my follow-up Master's studies in Environmental Chemistry, back when Professor Holoubek was still leading the institute. I wrote my thesis under Klára Hilscherová and simply stayed here ever since, except for a break during maternity leave. I have been part of her team the whole time, focusing on endocrine disruptors.&nbsp;What has changed over the years is the focus of our research. We used to study surface waters, particularly the presence of substances with endocrine-disrupting potential and their effects on organisms. Today, the research has shifted more toward humans and the interactions between chemical substances and the endocrine system.&nbsp;</span></p><p><span><strong>Tereza:</strong></span><strong> </strong><span>I came from a completely different field. I studied molecular biology and worked on chronic lymphocytic leukemia for my Master's thesis at CEITEC. I used genetic engineering methods such as CRISPR/Cas9 for gene editing—activating, suppressing, or completely removing genes.&nbsp;At the time, Klára Hilscherová’s group needed to create cellular models with increased production of deiodinase enzymes, which I still work on today. I had heard only positive things about the team, and when I finished my Master's degree, I wanted to move beyond my original field. The connection between hormones and neurodevelopment attracted me, and RECETOX became the obvious choice.&nbsp;</span></p><h5><strong>So you are in the same research team. How do you collaborate?&nbsp;</strong></h5><p><span><strong>Tereza:</strong></span><strong> </strong><span>We sit in the same office. And that’s probably the main thing (laughs).&nbsp;</span></p><p><span><strong>Jaroslava:</strong></span> <span>We discuss things a lot. Some mechanisms repeat across experiments, so we talk about how each of us evaluates data and why. We inspire each other, even though we do not directly collaborate on the same laboratory work.&nbsp;</span></p><h5><strong>How does your work differ?&nbsp;</strong></h5><p><span><strong>Tereza:</strong></span><strong> </strong><span>Our team is highly interconnected. We all work on developing assays for detecting endocrine-disrupting substances, specifically those affecting thyroid hormones. However, each of us focuses on a different part of the system.&nbsp;I focus on deiodinase enzymes, which regulate the activation and deactivation of hormones. Recently, I have been spending more time analyzing data and designing experiments than working directly in the laboratory.&nbsp;</span></p><p><span><strong>Jaroslava:</strong></span><strong> </strong><span>Within the team, I mainly participate in testing different types of samples—from individual chemicals and mixtures to real environmental samples such as household dust—using the assays mentioned above.&nbsp;It is important to note that chemicals can affect the hormonal system at different levels. They can bind to or block nuclear receptors, affect hormone transport in the bloodstream, or interfere with hormone metabolism. In the past, only one part of this process was monitored. Today, we aim to cover the full spectrum of these interactions.&nbsp;</span></p><p><span><strong>Tereza:</strong></span><strong> </strong><span>And that is crucial. A substance may appear negative in one test, yet affect another part of the system, producing a significant impact on the organism. That is why current research focuses on the entire range of possible mechanisms.&nbsp;</span></p><h5><strong>Why did you choose endocrine disruptors as your research topic?&nbsp;</strong></h5><p><span><strong>Jaroslava:</strong></span> <span>For me, it was more or less a coincidence while choosing my thesis topic. But it turned out to be a fascinating area, so I stayed with it.&nbsp;</span></p><p><span><strong>Tereza:</strong></span> <span>I had been interested in it for a long time. I also like that our work has a concrete outcome—we develop tests that can realistically be used in decision-making regarding the safety of chemical substances. Compared to my previous research, I feel this work has a much more direct application.&nbsp;</span></p><h5><strong>What about your PhD studies?&nbsp;</strong></h5><p><span><strong>Jaroslava:</strong></span><strong> </strong><span>My PhD studies are currently on hold. After maternity leave, I returned as a laboratory technician, and my project work gradually shifted away from my original doctoral topic.&nbsp;</span></p><p><span><strong>Tereza:</strong></span><strong> </strong><span>I am currently in the fourth year of my PhD studies. I also completed a research stay in Germany, where I learned to work with neuronal cells.&nbsp;As part of my studies, I focus mainly on deiodinases—the enzymes that act as the “on and off switches” of thyroid hormones. We are developing a test that can measure the effects of chemical substances on their activity.&nbsp;In addition, we are now studying how these substances affect neurons and their development, because proper thyroid hormone levels are crucial for nervous system development.&nbsp;</span></p><h5><strong>What is the most challenging aspect of laboratory work?&nbsp;</strong></h5><p><span><strong>Tereza:</strong></span><strong> </strong><span>Probably unpredictability. Cells do not always grow as expected, instruments have their bad days, and human error also plays a role. The important thing is to document all mistakes and complications and learn from them.&nbsp;</span></p><p><span><strong>Jaroslava:</strong></span> <span>I agree with Tereza. The biggest challenge is unpredictability. Cells are living systems and do not always behave the same way.&nbsp;</span></p><h5><strong>Can a career in science be combined with parenthood?&nbsp;</strong></h5><p><span><strong>Jaroslava:</strong></span> <span>At RECETOX, definitely. There is a great deal of flexibility, and managers are very supportive. When necessary, it is possible to work from home. We help each other a lot within the team.&nbsp;</span></p><p><span><strong>Tereza:</strong></span> <span>It also depends on the type of work and whether it can be planned according to your needs. Our team collaborates closely and tries to help one another whenever necessary. That is key. And when needed, data analysis and manuscript writing can also be done from home.&nbsp;</span></p><h5><strong>Is your work more routine or highly varied?&nbsp;</strong></h5><p><span><strong>Jaroslava:</strong></span> <span>Very varied. Laboratory work alternates with computer-based work. We are constantly learning new things. It is definitely not repetitive or boring, and that is what I enjoy about it.&nbsp;</span></p><p><span><strong>Tereza:</strong></span> <span>The only routine in my work is taking care of cell cultures. Otherwise, the work is constantly changing. That is also what I enjoy most about it.&nbsp;</span></p><h5><strong>What are the biggest challenges in your work?&nbsp;</strong></h5><p><span><strong>Jaroslava:</strong></span> <span>Data evaluation. Measuring something is one thing; evaluating and interpreting it correctly is another.&nbsp;</span></p><p><span><strong>Tereza:</strong></span> <span>Implementing new methods. And also those moments when you do everything correctly according to a published protocol, yet the results still do not match expectations.&nbsp;In science, you have to learn to accept that not everything works the first time. The important thing is not to give up, to continue optimizing, and to keep hoping for success.&nbsp;</span></p><h5><strong>What role should open science play?&nbsp;</strong></h5><p><span><strong>Tereza:</strong></span><strong> </strong><span>A huge one. If we had access to detailed protocols and original datasets, it would save enormous amounts of time and money during the implementation and optimization of new methods. Scientific papers often omit details that are actually critical for experimental success.&nbsp;</span></p><p><span><strong>Jaroslava:</strong></span><strong> </strong><span>It is also important to share failures. A negative result is still a result, and it can provide valuable information.&nbsp;</span></p><h5><strong>You are both mothers. Does your work influence your personal lives?&nbsp;</strong></h5><p><span><strong>Jaroslava:</strong></span><strong> </strong><span>Absolutely. For example, we pay close attention to washing fruit because of chemical treatments. I encourage my children to think about what they consume, what it contains, and where it comes from. We try to avoid plastics and consistently separate waste.&nbsp;</span></p><p><span><strong>Tereza:</strong></span> <span>Definitely. Even before having a child, I paid close attention to the composition of cosmetics, food, and other products. After becoming a parent, my caution multiplied.&nbsp;I was especially careful during pregnancy because a developing organism is most sensitive to harmful substances. Sometimes relatives raise their eyebrows at this, but when we have information firsthand, we cannot simply ignore it.&nbsp;</span></p><p><span><strong>Jaroslava:</strong></span> <span>We also pay attention to toys, clothing, and materials used in the home. It is a kind of professional deformation (laughs).&nbsp;</span></p><h5><strong>How can your research contribute from a broader perspective? How can others build upon it?&nbsp;</strong></h5><p><span><strong>Jaroslava:</strong></span> <span>We are gradually optimizing methods that may eventually be used routinely. That is probably our greatest contribution.&nbsp;</span></p><p><span><strong>Tereza:</strong></span> <span>Yes, our methods could help identify undesirable effects of substances before they reach the market, potentially leading to regulation of their production.&nbsp;</span></p><h5><strong>Would you like to continue working on this topic in the future?&nbsp;</strong></h5><p><span><strong>Jaroslava Večerková:</strong></span><strong> </strong><span>The topic of endocrine disruption is extremely interesting and very broad. The direction of research may change depending on current projects, but I would like to remain part of the team.&nbsp;</span></p><p><span><strong>Tereza Nešporová:</strong></span> <span>I am very interested in uncovering how substances affect human health, and I believe I will want to continue working in this area. I like how dynamic the research is and that it has direct practical applications. I do not mind change, but I am happy within this team and would like to stay. We work together as a well-coordinated group.&nbsp;</span></p>

Researchers Tereza Nešporová and Jaroslava Večerková: In science, we must learn to work with failure

<div><p><strong>Protein purification is a critical step in biopharmaceutical research, proteomics, and industrial biotechnology. It involves isolating a specific protein from complex biological mixtures while preserving its native structure and activity. Traditional methods such as precipitation or dialysis lack the precision required for high-purity protein isolation, especially when dealing with therapeutic targets or complex protein assemblies.</strong></p><p>High-performance liquid chromatography (HPLC) protein purification has transformed this field by providing high resolution, reproducibility, and speed, making it indispensable for both analytical and preparative workflows.</p></div><div><div><div><div><h3><strong>What is HPLC Protein Purification?</strong></h3><p>HPLC purification is based on the principle of selective interactions between proteins and the stationary phase under high-pressure liquid flow. The separation relies on differences in size, charge, hydrophobicity, or affinity of proteins toward the stationary phase material.</p><p>Unlike conventional low-pressure methods such as gel filtration or affinity batch purification, HPLC purification operates under high pressure (up to 6000 psi), enabling faster separation with enhanced resolution and quantitative recovery. It also offers precise control over parameters like gradient composition, pH, and temperature, minimizing variability between runs.</p><p>In contrast to traditional column chromatography, HPLC uses smaller stationary phase particles (3–10 µm), resulting in increased surface area and sharper <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/how-to-reduce-peak-tailing-in-hplc"><strong>peak shapes</strong></a>, which are vital for protein purity assessment and scale-up purification.</p></div></div></div></div><div><div><div><div><h3><strong>Types of HPLC Used for Protein Purification</strong></h3></div></div></div></div><div><p>Different HPLC modes can be employed depending on the physicochemical properties of the target protein. Each method offers unique advantages in terms of selectivity and recovery.</p><h4><strong>Reversed-Phase HPLC (RP-HPLC)</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase"><strong>Reversed-Phase HPLC</strong></a> separates proteins based on hydrophobic interactions between the analyte and the stationary phase, typically C4, C8, or C18 silica-based columns. Proteins are eluted using gradients of organic solvents (usually acetonitrile or methanol) in the presence of volatile acids like formic or trifluoroacetic acid (TFA).</p><p>This method provides exceptional resolution and is commonly used for protein fragment purification, and analysis of recombinant proteins. For example, reversed-phase HPLC is routinely employed in peptide therapeutic workflows, such as the<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an59280424_w_fc6c3ac6f0.pdf"><strong> Tirzepatide Preparation and Purification </strong></a>process, where purity and recovery are critical to product efficacy. However, due to exposure to organic solvents, RP-HPLC may cause partial denaturation, limiting its use for native proteins.</p><h4><strong>Ion Exchange HPLC (IEX-HPLC)</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec"><strong>IEX-HPLC</strong></a> separates proteins based on net surface charge. Cation exchange (with negatively charged stationary phases) binds positively charged proteins, while anion exchange does the reverse.</p><p>By gradually increasing salt concentration or changing the mobile phase pH, proteins are eluted according to their isoelectric points (pI).By gradually increasing salt concentration or changing the mobile phase pH, proteins are eluted according to their isoelectric points (pI).</p><p>This approach is highly suitable for native purification, protein isoform analysis, and charge variant characterization essential steps in therapeutic protein production.</p><h4><strong>Size Exclusion Chromatography (SEC-HPLC)</strong></h4><p>SEC separates molecules based on their hydrodynamic radius. Larger proteins elute first, as they are excluded from the pores of the stationary phase, while smaller molecules penetrate and elute later.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec"><strong>SEC-HPLC</strong></a> is non-denaturing and ideal for determining protein molecular weight, oligomeric state, and aggregate content. It is frequently used as a final polishing step following ion exchange or affinity chromatography.</p><h4><strong>Affinity HPLC</strong></h4><p>Affinity-based HPLC leverages specific biological interactions between the target protein and a ligand immobilized on the stationary phase (e.g., antibodies, metal ions, or tags such as His-tag or GST).</p><p>When integrated into an HPLC system, affinity chromatography provides high selectivity and purity (&gt;95%) in a single step, often used in recombinant protein purification and biomarker capture applications.</p></div><div><div><div><div><h3><strong>Benefits of Using HPLC for Protein Purification</strong></h3><p>HPLC-based protein purification combines the analytical precision of chromatography with the preparative capability needed for research and biomanufacturing. The main advantages include:</p><h5><strong>Benefit &nbsp;/ &nbsp;Description</strong></h5><p><strong>High Resolution and Selectivity:</strong></p><ul><li>Smaller stationary phase particles and optimized flow dynamics allow HPLC to resolve proteins differing by minimal physicochemical properties. This precision is vital for distinguishing isoforms, mutants, and post-translationally modified variants.</li></ul><p><strong>Accuracy and Reproducibility</strong></p><ul><li>Automated systems maintain stable flow rates, gradient control, and detection sensitivity, ensuring reproducible results essential for regulated biopharmaceutical workflows.</li></ul><p><strong>Speed and Efficiency</strong></p><ul><li>Modern UHPLC systems equipped with fully porous or core–shell particles achieve faster separations without sacrificing resolution, reducing run times by up to 50% compared to classical methods.</li></ul><p><strong>Compatibility and Scalability</strong></p><ul><li>HPLC systems are highly adaptable, supporting both analytical-scale characterization and preparative-scale purification. Methods developed at microgram scale can be seamlessly transferred to milligram or gram scales used in process development.</li></ul><div><div><div><div><h3><strong>Factors Affecting the Efficacy of Protein Purification by HPLC</strong></h3></div></div></div></div><div><p>Optimizing HPLC for protein purification requires balancing purity, recovery, and throughput. The following parameters significantly influence results:</p><ul><li><strong>Purification goal:</strong> Define whether the priority is yield, purity, or throughput. Analytical separations prioritize resolution, while preparative runs emphasize recovery and process speed.</li><li><strong>Protein solubility and stability:</strong> Unstable or aggregation-prone proteins may require low temperatures or additives (e.g., glycerol, mild detergents).</li><li><strong>Column chemistry and particle size:</strong> Select appropriate stationary phase chemistry (silica, polymeric, or mixed-mode) and particle size to suit molecular weight and hydrophobicity.</li><li><strong>Mobile phase pH and ionic strength:</strong> pH should be optimized near but not at the protein’s pI; salt gradients influence both resolution and elution strength.</li><li><strong>Flow rate and gradient profile:</strong> Steeper gradients shorten run time but may compromise separation quality.</li><li><strong>Purified protein final form:</strong> Consider buffer composition and concentration for storage or downstream assays to maintain bioactivity.</li></ul></div><div><div><div><div><h3><strong>Best Practices for Protein Purification by HPLC</strong></h3></div></div></div></div><div><p>Following good chromatographic practices ensures consistent, high-quality results:</p><ul><li><strong>Column conditioning and storage:</strong> Always equilibrate new <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=48&amp;manufacturerItems=50"><strong>HPLC columns</strong></a> with 10 column volumes of buffer; store in appropriate solvent containing preservatives.</li><li><strong>Avoid protein precipitation:</strong> Maintain appropriate ionic strength and, when native state is desired, avoid exposure to denaturing solvents or high organic content.</li><li><strong>Prevent sample loss:</strong> Use low-protein-binding tubing and vials; minimize dead volume in the system.</li><li><strong>Troubleshooting:</strong><ul><li>Peak fronting - may indicate column overloading.</li><li>Peak broadening – may indicate column channeling.</li><li>Carryover – reduce by extending wash cycles or using stronger regeneration solvents.</li><li>Clogging – prefilter samples (0.22 µm) and periodically flush the system.</li></ul></li></ul></div></div></div></div></div>

Protein Purification by HPLC: Methods, Benefits, and Best Practices

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 6th July 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you application notes by <strong>Agilent Technologies </strong>and<strong> Metrohm </strong>and posters by<strong> Shimadzu/ASMS, Thermo Fisher Scientific/ASMS </strong>and <strong>Waters Corporation/ASMS!</strong></p><h3><strong>1. Agilent Technologies: Determination of 41 Per- and Polyfluoroalkyl Substances (PFAS) in Cosmetics</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_captiva_emr_pfas_food_ii_cartridge_5994_9111en_agilent_be91861646.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Per- and polyfluoroalkyl substances (PFAS) are synthetic compounds composed of highly stable carbon–fluorine (C–F) bonds, giving them exceptional thermal and chemical stability, leading to poor environmental degradability. PFAS have been used in cosmetics to provide water resistance, oil repellency, or application smoothness. The unintentionally presented PFAS residues in raw materials may remain in finished cosmetic products, and can be absorbed through the skin. Lip products and spray type products can also lead to inhalation or oral exposure to residues.<sup>1,2</sup>&nbsp;</p><p>Agilent Captiva EMR PFAS Food cartridges are designed to provide streamlined and comprehensive matrix removal after QuEChERS extraction for complex sample matrices, demonstrating effective matrix cleanup efficiency and excellent performance for quantitative PFAS analysis.<sup>3,4</sup> In this study, we performed QuEChERS extraction followed by cleanup with Captiva EMR PFAS Food II cartridges for PFAS analysis in five types of cosmetics.</p><h4><strong>Experimental</strong></h4><h5><strong>Equipment and materials&nbsp;</strong></h5><p>The study was performed using an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+III"><strong>Agilent 1290 Infinity III LC</strong></a> system consisting of an Agilent 1290 Infinity III high-speed pump (G7120A), an Agilent 1290 Infinity III multisampler (G7167B), and an Agilent 1290 Infinity III multicolumn thermostat (G7116B). The LC system was coupled to an <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6495D"><strong>Agilent 6495D triple quadrupole LC/MS system</strong></a> (G6495D) equipped with an Agilent Jet Stream Electrospray ion source. Agilent MassHunter Workstation software was used for data acquisition and analysis.&nbsp;</p><p>The 1290 Infinity III LC system was modified using an Agilent InfinityLab PFAS Analysis HPLC conversion kit (part number 5004-0006), including an Agilent InfinityLab PFC delay column, 4.6 × 30 mm (part number 5062-8100). Chromatographic separation was performed using an Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=ZORBAX+RRHD+Eclipse+Plus"><strong>ZORBAX RRHD Eclipse Plus</strong></a> C18, 95 Å, 2.1 × 100 mm, 1.8 µm (part number 959758-902).</p><h4><strong>Results and discussion&nbsp;</strong></h4><h5><strong>Chromatography and calibration curve linearity&nbsp;</strong></h5><p>Figure 2 shows the overlaid chromatograms for 41 PFAS calibration standards, including 0.05, 0.1, 0.2, 0.5, 1.0, 2.0, 5.0, and 10.0 ng/mL in ACN. The table on the right shows the peaks identification and corresponding retention time. Across 41 PFAS compounds, R² values ranged from 0.9940 to 0.9998, indicating excellent linearity for all analytes' calibration curves across the calibration range of 0.05 to 10.0 ng/mL.</p><h5><strong>PFAS detection in real cosmetic samples&nbsp;</strong></h5><p>Of the 15 cosmetic products assessed, one eyeshadow sample was detected with a trace level of positive PFAS analytes, including PFHeA, PFHpA, PFNA, and PFOA, at about 0.1 ng/g in the sample. Figure 5 shows the method blanks and positive detection of PFHpA and PFNA in this eyeshadow&nbsp;product.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>A simplified, rapid, and reliable method using QuEChERS extraction followed by EMR mixed-mode passthrough cleanup using the Agilent Captiva EMR PFAS Food II cartridge and LC/MS/MS detection was developed for 41 PFAS analysis in cosmetics.</p><h3><strong>2. Metrohm: brightRC – Advanced brightener analysis with a response curve</strong></h3><p><strong>Next-level brightener determination in nonlinear systems</strong></p><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_v_242_download_83376b2923.pdf"><strong>Full PDF for download</strong></a></li></ul><h4><strong>WHAT IS BRIGHTRC?&nbsp;</strong></h4><p>brightRC is a response curve calibration approach for the determination of brightener-type additives by CVS or CPVS (Cyclic Pulse Voltammetric Stripping), hence the name «brightRC». This technique is suitable to measure brightener content in copper baths for plating. The method is based on signal normalization using the electrolyte value, which is the response for the virgin makeup solution (VMS) saturated with suppressor, and brightener defined as Q0 in the viva software. All recorded signals are divided by Q0 and the normalized values are plotted against concentration to generate an external calibration curve. This calibration can then be used for routine sample determination without repeated standard additions.&nbsp;</p><p>The sample is simply measured using the same method as the calibration, signals are normalized and evaluated using the mathematically fitted calibration curve. By decoupling calibration from sample measurement, brightRC removes the need for repeated standard additions with each sample measurement, enabling faster and more efficient routine analysis.&nbsp;</p><p>The brightRC calibration approach supports flexible regression models including quadratic and nonlinear regression. This allows reliable quantification of additive systems that do not exhibit a wide linear working range or exhibit nonlinear response characteristics, thereby expanding the range of applicable brightener chemistries.&nbsp;</p><p>One important detail that further differentiates brightRC from the MLAT approach relates to normalization of the signal for calibration and sample analysis. This normalization approach improves measurement robustness as variations in absolute signal intensity naturally occurring over the working day are mathematically compensated.</p><h4><strong>HOW DOES BRIGHTRC WORK IN PRACTICE?&nbsp;</strong></h4><p>The brightRC workflow separates calibration and sample analysis. It can be implemented in different setups (from manual to fully automated) depending on the additive system and concentration range. After recording the electrolyte value, calibration standards are measured to establish the calibration curve, otherwise known as the response curve. Once the calibration is stored, samples are determined with the same CVS or CPVS method, without further standard additions. The sample concentration is automatically calculated from the normalized signal using the calibration curve.&nbsp;</p><p>For sample measurement, viva 4.0 provides two practical approaches:&nbsp;</p><ol><li>«brightRC with solution exchange»&nbsp;</li><li>«brightRC with sample dilution» Both variants follow the same calibration principle and differ only in the sample handling strategy.</li></ol><h4><strong>WHEN TO USE BRIGHTRC?&nbsp;</strong></h4><p>brightRC is ideal for routine brightener control in stable plating baths where high sample throughput is required. It is particularly suitable when matrix conditions are consistent and electrode fouling is limited. The approach is especially advantageous for additive systems exhibiting nonlinear signal behavior. If strong matrix variations, significant breakdown products, or severe electrode fouling are expected and a linear correlation between signal and analyte concentration is guaranteed, standard addition techniques such as MLAT are preferred because they may provide additional robustness.</p><h3><strong>3. Shimadzu / ASMS: PFAS Analysis of Leachate: Evaluating Key Sample Preparation and LC–MS/MS Parameters for Improved Performance</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/WP_255_PFAS_Analysis_of_Leachate_Evaluating_Key_Sample_Preparation_and_LC_MSMS_Parameters_for_Improved_Performance_1114ee264c.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Landfill leachate is a highly complex and variable matrix that poses significant challenges for the analysis of per- and polyfluoroalkyl substances (PFAS). Although U.S. Environmental Protection Agency Method 1633A provides a standardized framework for PFAS quantitation,1 analytical performance remains strongly influenced by sample preparation, chromatographic separation, and mass spectrometric conditions. Landfill leachate samples were extracted using WAX SPE following EPA 1633A and analyzed by LC–MS/MS using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8060"><strong>Shimadzu LCMS-8060</strong></a><strong> </strong>under negative ESI conditions. This study evaluates the key factors governing LC–MS/MS sensitivity, signal response, and reproducibility in landfill leachate, including a systematic assessment of SPE, chromatographic behavior, and ionsource conditions across PFAS classes (Figure 1).</p><h4><strong>Results&nbsp;</strong></h4><p>Chromatographic performance was evaluated across four C18 columns (A–D) using peak area, height, asymmetry, and HETP. Two columns produced a higher signal and improved peak shape, while the remaining columns showed greater efficiency. Under repeated batches followed by storage in acetonitrile, the Shimadzu GIST column produced a consistent signal (≤15% change), whereas another column showed 20–50% signal loss, particularly for long-chain PFAS and precursors, indicating greater susceptibility to matrix accumulation (Figure 2).</p><h4><strong>Conclusion&nbsp;</strong></h4><ul><li>PFAS analytical performance was dependent on compoundclass-dependent extraction, chromatography, and ionization behavior.&nbsp;</li><li>GIST demonstrated lower signal variability under repeated matrix exposure, with lower signal degradation than alternative C18 phases.</li><li>Neutral PFAS showed poor retention on WAX sorbents, requiring optimization beyond standard EPA 1633A extraction conditions.&nbsp;</li><li>PFCA response was more influenced by gas flow conditions, whereas PFSA response was more sensitive to source temperature conditions.</li></ul><h3><strong>4. Thermo Fisher Scientific / ASMS: Ultra-high throughput proteomics at &gt;500 samples/day using advanced </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398"><strong>Vanquish Neo UHPLC</strong></a><strong> methods with </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959"><strong>Orbitrap Astral Zoom MS</strong></a></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/po_004708_lsms_ultra_high_throughput_vanquish_astral_proteomics_po004708_asms2026_na_en_f6499c3eec.pdf"><strong>Full PDF for download</strong></a></li></ul><p>High-throughput mass spectrometry is increasingly critical for large-cohort proteomics, population-scale studies, and screening applications. While established LC–MS platforms routinely support several hundred samples per day, further increases in throughput are often limited by chromatographic stability, robustness, and reproducibility under ultra-short gradient conditions, as well as the acquisition speed of the mass spectrometer. Here, we describe an advanced ultra-high-throughput LC–MS methodology developed on the Thermo Scientific Vanquish Neo UHPLC system and Thermo Scientific Orbitrap Astral Zoom mass spectrometer. The goal of this work was to extend the practical throughput limits of LC–MS while achieving deep proteome coverage and maintaining run-to-run reproducibility.</p><h4><strong>Materials and methods</strong></h4><h5><strong>LC-MS analysis&nbsp;</strong></h5><p>Samples were separated on a Thermo Scientific 150 µm × 70 mm C18 column (prototype, not commercially available yet) operated at 4 µL/min using a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/398"><strong>Vanquish Neo UHPLC system</strong></a> in NanoCap Trap&amp;Elute configuration at room temperature. Prototype methods allowing for reduced LC duty cycles (approx. 1 min of sample pickup/loading and integrated trap-column offline wash) were applied, the standard gradients were adjusted to 1 – 3 min. The Thermo Scientific PepMap Neo 5 µm C18 300 µm × 5 mm cartridge was used as trap column and the separation column connected with a 15 µm ID capillary Thermo Scientific EASY-Spray emitter (ES994) to an EASY-Spray source. The <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1959"><strong>Orbitrap Astral Zoom MS</strong></a> (ICSW AST 2.2) was operated in a dataindependent acquisition (DIA) mode, MS method parameters are given in Table 1. Comparative data for the chemoproteomics samples at lower throughputs (60-300 SPD) were acquired using an EASY-Spray 150 µm × 150 mm C18 column (ES906), as described before.&nbsp;</p><h5><strong>Data analysis&nbsp;</strong></h5><p>The raw data files were processed with Spectronaut® 20.5 software (Biognosys AG), <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782"><strong>Thermo Scientific Proteome Discoverer </strong></a>3.3 SP1 with <strong>CHIMERYS 5.0 </strong>or DIA-NN Enterprise 2.3.2 (Aptila Biotech GmbH) nodes using default settings as specified in the individual figures. The data was searched against the Homo sapiens SwissProt database (TaxID=9606: 42,252 sequences) and contaminants FASTA file. Peptide numbers correspond to unique stripped peptides</p><h4><strong>Conclusions&nbsp;</strong></h4><ul><li>The advanced Vanquish Neo UHPLC methodology in combination with the Orbitrap Astral Zoom MS delivered highly reproducible, deep and precise proteome profiling across ultra-high-throughput regimes (600 SPD) exceeding current field benchmarks.&nbsp;</li><li>The workflow enables rapid chemoproteomic screening, as demonstrated with activity-based protein profiling (ABPP) samples, reliably identifying enriched putative target proteins of an antibiotic small molecule probe.</li></ul><h3><strong>5. Waters Corporation / ASMS: Assessing Relative Response of Four European-Regulated PFAS in Human Serum Using Cyclic Ion Mobility MS</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009410_c98e7c87ec.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>The poster presents an LC-Cyclic-IMS-MS approach for assessing four European-regulated PFAS—PFOS, PFOA, PFNA, and PFHxS—in human serum. The study focuses on environmental exposure monitoring in Ghanaian firefighters and e-waste handlers, using ion mobility mass spectrometry to add separation power and improve identification confidence in complex biological matrices. The work is motivated by increasing regulatory concern around PFAS toxicity, bioaccumulation, and the need for reliable exposure assessment in human biofluids.</p><p>The experimental workflow combined reversed-phase LC with a quadrupole-cyclic ion mobility-time-of-flight MS system (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/202"><strong>SELECT SERIES</strong> <strong>Cyclic IMS</strong></a>). Human serum samples were prepared using SPE µElution plates with a polymeric reversed-phase/weak anion exchange sorbent. The LC system was modified with PFAS-free components and an isolator column to reduce background contamination, which is especially important for trace-level PFAS analysis. Identification was supported by retention time, accurate mass, isotopically labelled internal standards, and collision cross section values.</p><p>The results showed detectable levels of the four regulated PFAS in both occupational groups, with a combined detection rate of 83% across human serum samples and NIST reference standards. Firefighter samples generally showed higher concentration ranges than e-waste handler samples, especially for PFOS, PFOA, PFNA, and PFHxS. For example, PFNA reached up to 5.713 ng/mL in firefighter serum, compared with up to 1.513 ng/mL in e-waste handler serum.</p><p>Overall, the poster concludes that LC-Cyclic-IMS-MS provides a highly specific strategy for PFAS analysis in complex biological samples. Ion mobility and CCS values improve confidence in trace-level identification and help distinguish target PFAS from co-eluting matrix background. The authors suggest that this approach can support both targeted PFAS monitoring and broader non-targeted screening of known and emerging PFAS, while also helping future studies connect exposure data with environmental fate and potential toxicological effects.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 28, 2026

Article

Product

Application

Academy

Scientists

Poster

RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

RECETOX - Centrum pro výzkum toxických látek v prostředí

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Mettler-Toledo

Agilent Technologies

SelectScience

Waters Corporation

<h3><strong>Solving Problems That Matter - a Waters webinar series featuring USP speakers</strong></h3><h4><strong>Episode 8: Lipid nanoparticles</strong></h4><div class="summary-section section"><p id="isPasted"><span class="summary" name="summary">Developing safe, compliant and efficacious lipid nanoparticles (LNP) requires adequate testing of the individual lipid components and impurities. In this webinar Anni Chang from the USP Excipient team will share current and future excipient solution approaches and describe how the USP provides support for stakeholders in discovery and early-stage development. Then Duanduan Han will discuss the benefits of mass detection for critical impurities analysis, and Empower software workflows for compliant and straightforward method migration from development to QC labs. Duanduan will also highlight how multi-angle light scattering (MALS) detection can be used to characterize the LNP.</span></p><h5><span class="summary" name="summary"><strong fr-original-style="">Key learning objectives</strong></span></h5><ul><li><span class="summary" name="summary">Explore current and future USP solutions for lipid nanoparticles (LNP)</span></li><li><span class="summary" name="summary">Learn how mass detection and Empower software-based workflows expedite and enhance routine testing of LNP components and impurities</span></li><li><span class="summary" name="summary">Discover orthogonal insights enabled by multi-angle light scattering detection (MALS)</span></li></ul><h5><span class="summary" name="summary"><strong fr-original-style="">Who should attend</strong></span></h5><p>Scientists involved with lipid nanoparticle analysis</p><blockquote><p><span class="sp-name"><strong>Presenter: </strong>Anni Chang, Ph.D. (</span><span class="sp-title">Sr Scientist II, Excipients Unit, </span><span class="sp-company">US Pharmacopeia)</span></p><p><span class="sp-description">Dr. Anni Chang is currently a Senior Scientist II in the Excipients unit at the United States Pharmacopeia (USP). In her current role, she is responsible for developing, revising, and modernizing USP monographs. One of her focus areas is lipid excipients used in nanomaterial carriers.</span></p><p><span class="sp-description">Dr. Chang has more than 15 years of experience in the biotech/pharmaceutical industry in analytical method development, validation, and transfer. She received her Ph.D. in Chemistry from University of Maryland, College Park, Maryland, USA. Dr. Chang is also active in the American Association of Pharmaceutical Scientists and serves as a Member Engagement Manager in the Lipid Based Drug Delivery community.</span></p><p><span class="sp-name"><strong>Presenter: </strong>Duanduan Han, Ph.D. (</span><span class="sp-title">Senior Scientist QA/QC Biologics Solutions group, </span><span class="sp-company">Waters Corporation)</span></p><p><span class="sp-company">Duanduan is a Senior Scientist in the QA/QC Biologics Solutions group at Waters Corporation headquarters in Milford, MA. She joined Waters in 2021, and she has focused on LC method development in composition analysis, impurity profiling, and raw material screening of lipid nanoparticle components and products. Additionally, she has explored other subjects of pharmaceutical analysis including surfactant, protein, and peptide. Prior to joining Waters, she received her PhD in chemical engineering from Texas A&amp;M University.</span></p><p><span class="sp-name"><strong>Presenter: </strong></span>Panelist: Martin Kurnik, Ph.D. (Senior Scientist, Analytical Sciences, Waters | Wyatt Technology​)</p><p><span class="sp-description">Martin Kurnik is a senior scientist in the Analytical Sciences team at Waters | Wyatt Technology. He helps customers identify and develop the best solutions for their analytical needs by leveraging his expertise in multi-angle light scattering and dynamic light scattering as well as a wide range of other leading techniques for biomolecular characterization. His postdoctoral research in protein folding and misfolding biophysics focused on understanding and preventing misfolding in bulk solution and on artificial surfaces used in medicine and biotechnology, and he has also developed protein- and aptamer-based electrochemical biosensors for continuous real-time measurements in vitro and in vivo. Martin received his M.Sc. in Chemistry and Ph.D. in Biochemistry from Stockholm University, Sweden.</span></p><p><span class="sp-title"><strong>Moderated by:</strong> Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, </span><span class="sp-company">Waters Corporation)</span></p><p><span class="sp-description">Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.</span></p></blockquote></div>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

Lipid nanoparticles

Solving Problems That Matter - a Waters webinar series featuring USP speakers. Episode 8: Complex excipients: Lipid nanoparticles.

Complex excipients: Lipid nanoparticles

<p><strong>Data management and manual transcription of critical process data remain one of the major challenges and potential sources of errors in the analytical laboratory.</strong></p>
<p>As a lot of the laboratory processes evolve around sample preparation for HPLC or GC, with crucial weighing and preparative steps, this webinar aims to showcase some seamless data management and transfer solutions, from balance to LC. The LabX™ balance software and OpenLab CDS platform can enable various levels of integrated data management, while also improving productivity and offering a high degree of data integrity.</p>
<p>Join the METTLER TOLEDO and Agilent teams for a webinar to understand how you can support digitalized and scalable sample preparation processes for better chromatography results. </p>
<p>Program Overview</p>
<ul>
<li><p><strong>Introduction to the digital lab</strong></p>
</li>
<li><p><strong>Improving analytical sample preparation workflows</strong></p>
</li>
<li><p><strong>Live demonstration of seamless data transfer options</strong></p>
</li>
<li><p><strong>Q&amp;A with presenters</strong></p>
</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Andrea Brütsch (Head Business Development Initiatives)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Kevin Koelliker (Agilent Technologies, Application Engineer)</p>
</blockquote>


Mettler Toledo: Automated Data Management for LC/GC Workflows

Automated Data Management for LC/GC Workflows

How can you support digitalized and scalable sample preparation processes for better chromatography results.

<p><strong>In the second part of the stationary phases webinars, we look at other separation modes: IEX, SEC, ligand exchange and HILIC and what we think they are best suited for.</strong></p><blockquote><p><strong>Presenter:</strong> Ceci<span class="sp-name">lia Mazza (</span><span class="sp-title">Product Specialist, EMEA IDO - Chemistries &amp; Suppliers, </span><span class="sp-company">Agilent)</span></p><p><span class="sp-description">With more than 20 years of professional experience, Cecilia is presently the Product Specialist for columns and supplies at Agilent. Supporting distributors and laboratory users in the analysis and purification of small and large molecules, for a wide range of LC and GC applications as well as sample preparation.</span></p><p><span class="sp-description">Cecilia holds a Ph.D. in Chemical Engineering from Rensselaer Polytechnic Institute in US.</span></p></blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Stationary Phases in HPLC - Part II

We look at other separation modes: IEX, SEC, ligand exchange and HILIC and what we think they are best suited for.

<p><strong>Heightened interest in mRNA therapeutics has accelerated the need for analytical methodologies that facilitate the production of supplies for clinical trials. This webinar will present a concise panel of chromatography-based stability-indicating assays for evaluating thermally stressed in vitro transcribed mRNA as part of a forced degradation study.</strong></p><p>Our expert speakers will demonstrate the application of these methods to evaluate the critical quality attributes (CQAs) of mRNAs as well as to detect intrinsic process- and product-related impurities.</p><h4>Key learning objectives</h4><ul><li><strong>Characterization of thermally stressed mRNA using a panel of chromatographic assays</strong></li><li><strong>Implementation of SEC-MALS to confirm transcripts reflect full-length sequence design</strong></li><li><strong>Platform 5’ cap/3’ tail analyses, circumventing antisense probe design</strong></li><li><strong>Design of QC-friendly methods fit for research, release, and comparability testing</strong></li></ul><h4>Who should attend?</h4><ul><li>QC lab technicians or lab assistants.</li><li>Lab managers of development, process development, or QC.</li><li>Directors/Senior directors of development, process development or QC</li><li>VP of Quality; VP of Development or VP of CMC.</li><li>Anyone interested in learning more about light scattering and its applications on mRNA characterization.</li><li>Industry and academic researchers working in gene therapy and biotherapeutics.</li><li>Any scientist in discovery, development, and quality control.</li></ul><blockquote><p><strong>Presenter: </strong>Jaclyn Cika (Scientist – Protein Biochemistry group, Regeneron)</p><p>Jaclyn Cika joined the Protein Biochemistry group at Regeneron Pharmaceuticals in 2019. She provides late-stage development and commercial support through the biophysical characterization of Regeneron antibody and AAV reference standards. She also oversees the qualification status of various SEC-MALS systems, and is responsible for SEC-MALS method qualification for commercial products. Additionally, she focuses on the biophysical characterization of nucleic acids, primarily using light scattering techniques, for early research and development support.</p><p><strong>Presenter:&nbsp;</strong>James Greco (Regional Account Manager, Wyatt Technology, Waters | Wyatt Technology)</p><p>James Greco is a Regional Account Manager at Wyatt Technology, bringing over 16 years of experience in the biopharma industry. He has a B.Sc. in Biology from Salve Regina University. He began his career at BMS, where he spent eight years specializing in biophysical characterization. In 2017, he joined Wyatt Technology as a Field Application Scientist, where he supported and provided advanced training to customers across various fields. He later transitioned to his current role as Regional Account Manager.</p><p><strong>Presenter:&nbsp;</strong>Charlie Carter (Life Sciences Editor, SelectScience)</p></blockquote>

SelectScience: Comprehensive chromatographic assessment of forced degraded in vitro transcribed mRNA

Comprehensive chromatographic assessment of forced degraded in vitro transcribed mRNA

The application of these methods to evaluate the critical quality attributes (CQAs) of mRNAs as well as to detect impurities.

Comprehensive chromatographic assessment of forced

<p><strong>The presence of per- and polyfluoroalkyl substances (PFAS) in food supply chains is a growing concern due to their environmental persistence and potential health risks.</strong></p><p>As awareness increases, the demand for effective regulations and advanced analytical methods for detecting PFAS in food is critical. In this webinar hosted by Agilent in association with AOAC India Section, we will explore the current PFAS contamination landscape, regulatory frameworks, and the latest analytical solutions.</p><p>The session will cover PFAS characteristics and the challenges they present to food safety. We will examine global and regional regulations, with a focus on evolving standards in India and the South Asia Pacific region. Also, the webinar will highlight advanced analytical approaches for different food matrices like seafood, eggs, meat, infant formula, packaging material, including sample preparation, instrumentation, and reporting essential for effective PFAS detection. Agilent's robust LC/MS and GC/MS PFAS testing solutions will be showcased along with the Captiva EMR sample preparation kit, demonstrating how they can help food manufacturers, exporters, and regulatory bodies ensure compliance with global standards.</p><h4>Learning outcomes:</h4><ul><li><strong>Comprehensive understanding of PFAS characteristics and the regulatory landscape across global and regional markets, including India and the South Asia Pacific.</strong></li><li><strong>Advanced analytical techniques for PFAS detection in food, covering sample preparation, instrumentation, and reporting methodologies.</strong></li><li><strong>Practical insights into implementing PFAS testing solutions to ensure compliance with international food safety standards.</strong></li></ul><h4>Who should attend:</h4><ul><li>Food scientists, analytical chemists, regulatory authorities, government lab personnel, industry professionals in food manufacturing, packaging, exporting, R&amp;D scientists, toxicologists, QA/QC analysts and managers, academicians, and researchers focused on PFAS analysis and food safety.</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Nick Birse (Lecturer, School of Biological Sciences, Queen's University, Belfast, Northern Ireland, UK)</p><p>Nick Birse is a Lecturer in Mass Spectrometry at Queen’s University Belfast, where he focuses on the use of mass spectrometry to solve key food integrity challenges, such as the measurement of chemical contaminants and natural toxins, fingerprinting foods to determine geographic origin and help detect fraud, or simply to measure key quality, flavour and taste attributes, all of which makes foods tastier, healthier and safer for consumers. Nick previously served as a Research Fellow within Prof Chris Elliott’s research group, assisting in the execution of Chris’s 2021 Agilent Thought Leader Award. He holds a PhD in Analytical Chemistry from Queen’s University Belfast.</p><p><strong>Presenter:&nbsp;</strong>Dr. Niladri Sekhar Chatterjee (Senior Scientist and DST Inspire Fellow, ICAR-Central Institute of Fisheries Technology, India)</p><p>Dr. Niladri has more than 10 years of experience in precision measurement of small molecules in food, biological, and environmental matrices. His doctoral research is on pesticide metabolism, and environmental fate in crop, soil, and water. His postdoctoral research is on seafood authentication using high resolution mass spectrometry-based metabolomics and molecular spectroscopy fingerprinting. He currently works as a Senior Scientist in the Biochemistry and Nutrition Division at ICAR Central Institute of Fisheries in Kochi. At CIFT, he has been instrumental in establishing a state-of-the-art analytical chemistry laboratory from scratch which is today one of the National Reference Laboratories. He has received external grants from the Science and Engineering Research Board (SERB) India, National Agricultural Science Fund, and FSSAI. In the NRL, he has developed and validated precision measurement methods for multiclass analysis of pesticides, antibiotic residues, and endocrine disruptors and is actively managing the ISO 17025 accreditation of the laboratory. His research work has resulted in several peer reviewed publications.</p><p><strong>Presenter:&nbsp;</strong>Dr. Lorna De-Leoz (Global Food Segment Director, Agilent Technologies, USA)</p><p>Lorna De Leoz has nearly 20 years of experience in the food industry. As the Global Food Segment Director, she collaborates with scientists to help develop accurate and reliable testing for food safety, quality, and authenticity. Lorna worked at the National Institute of Standards and Technology in the Mass Spectrometry Data Center for seven years. She completed her Ph.D. in Chemistry at University of California, Davis on milk oligosaccharides and glycan biomarkers analyses.</p></blockquote>

Agilent Technologies: PFAS in Food Supply Chains: Regulations and Analytical Approaches: in association with AOAC India Section

PFAS in Food Supply Chains: Regulations and Analytical Approaches: in association with AOAC India Section

PFAS characteristics and the challenges they present to food safety.

PFAS in Food Supply Chains: Regulations and Analytical

<p><strong>Nitrosamine Drug Substance-Related Impurities (NDSRI's) are a class of impurities that can form in drug substances and products. These impurities are of significant concern due to their potential carcinogenicity. Regulatory bodies, including the FDA, have established guidelines and acceptable intake limits to ensure the safety and efficacy of medications.</strong></p><p>NDSRI webinar series is a comprehensive three-part webinar series designed to provide valuable insights into the evolving landscape of analytical methods and regulatory requirements. This series will cover critical topics that are essential for professionals in the field.</p><p><strong>Key learnings:</strong></p><ul><li>Understanding Nitrosamine / NDSRI and their impact on drug safety.</li><li>Latest regulatory guidelines and requirements.</li><li>Navigating Nitrosamines with Agilent 21 CFR compliant mass Hunter Software.</li><li>A focus on components in chromatographic challenges, with examples of NDSRI methods.</li><li>Case studies and real-world applications.</li><li>Column and consumable selection and how it influences the analysis.</li><li>Best practice for method optimization, development, and automation.</li><li>and more</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Kuldeep Sharma (Sales Development Manager - Pharma, Agilent Technologies)</p><p>Kuldeep Sharma has over 19 years of experience in area/s of Bioanalytical research, Drug metabolism &amp; pharmacokinetics and Pharma regulatory compliance. He has accomplished PhD in Biotechnology. His professional journey covers over 12 years in Analytical research (associated with R&amp;D’s of Zydus Cadila, Lambda, Pliva and Jubilant Biosys) and last 7 years in analytical instruments sales, marketing &amp; business development. With over 21 research articles published in international Journals, He presents convention of continues learning.</p><p><strong>Presenter:&nbsp;</strong>Dr. Prasanth Joseph (Application Engineer - LC/MS, Agilent Technologies)</p><p>Prasanth is an experienced analytical instrumentation professional with a specialization in small molecule applications. Joining Agilent in 2015 as an Application Engineer for LC-MS/MS, he has 19 years of expertise in the field. Prasanth holds a Ph.D. in Environmental Science from Bharathiar University, India, and a master's degree in Business Administration from the University of Buckingham, UK. His extensive knowledge includes developing analytical methods for quantifying genotoxic impurities, analyzing extractables and leachables in pharma and food packaging, conducting bioavailability and bioequivalence studies, and more. Prasanth's proficiency extends to the analysis of phytochemicals, food residue screening, and environmental sample contaminants.</p><p><strong>Presenter:&nbsp;</strong>Mr. Ankush Sharma (Regional Marketing Program Manager- APAC (Pharma &amp; Biopharma), Agilent Technologies)</p><p>Ankush Sharma is a seasoned professional with over 12 years of extensive experience in sales and marketing across the pharmaceutical and biopharmaceutical sectors. His professional journey commenced with a strong educational foundation, earning a Master's degree in Biotechnology. Building upon this knowledge, he furthered his academic pursuits by obtaining a Master's in Business Administration with a specialization in Sales and Marketing, demonstrating his commitment to blending scientific understanding with strategic business acumen. For the past five years, he has been an integral part of Agilent, serving as the Regional Marketing Program Manager for the India and now Asia-Pacific (APAC) region for Pharma and Biopharma Markets.</p></blockquote>

Agilent: Nitrosamines & NDSRI's: Be sure with Agilent workflows

Nitrosamines & NDSRI's: Evolving Regulatory Landscape in Nitrosamines and NDSRI’s analysis.

NDSRI webinar series is designed to provide valuable insights into the evolving landscape of analytical methods and regulatory requirements.

Nitrosamines & NDSRI's: Be sure with Agilent workflows

<h4><strong>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</strong></h4><p>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.</p><h5><strong>Why attend?</strong></h5><ul><li><strong>Comprehensive Learning:</strong> Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li><strong>Expert Insights:</strong> Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li><strong>Regulatory Compliance:</strong> Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3><strong>Episode #1: Chromatography - UPLC | F&amp;E Summer School - Day 1 - Module 1</strong></h3><p>This section will provide an overview of the Waters reference material available to customer to help plan the best columns and chromatographic conditions for their LC-MS methods, using common environmental contaminants as an example. We highlight how to create LC-MS methods using the Acquisition Method Editor (AME) application within waters_connect™. Finally, this section will cover some of the most troubleshooting issues: contaminated blank peaks, poor peak shape, etc, and how to address them.</p><h5><strong>What you'll learn:</strong></h5><ul><li>Important factors for developing a UPLC method - considering analyte solubility, pKa and partitioning coefficient (Kow)</li><li>Selecting the most appropriate UPLC column for your analytes</li><li>Setting up LC-MS methods in the waters_connectTM console</li><li>Troubleshooting common contamination issues</li></ul><blockquote><p><strong>Presenter: </strong>Nicole Baumgarten (Sr Sales Applications Chemist, Waters Corporation)</p><p>Nicole is part of the Customer Success team at Waters with a focus on LC-MS/MS application and sales support. She works in this role for more than 9 years and gained experience in previous roles as analyst for biopharmaceuticals in process controls at Boehringer Ingelheim and research Assistent at Zentrum für Humangenetik und Laboratoriumsmedizin in Munich after finishing a Master of Chemical engineering at Hochschule Fresenius, Idstein.</p><p><strong>Presenter: </strong>Caroline Davis (Sr Sales Applications Chemist, Waters Corporation)</p></blockquote>

Waters Corporation: The Waters 2024 Virtual Summer School

Chromatography - UPLC

An overview of the Waters reference material available to customer to help plan the best columns and chromatographic conditions for their LC-MS methods.

Chromatography - UPLC 

<h4><strong>The Waters 2024 Virtual Summer School - An Educational Journey to Master Food &amp; Environmental Analysis</strong></h4><p>Unlock the full potential of LC-MS techniques with our immersive and educational sessions at the Waters Virtual F&amp;E Late Summer School 2024. From September 24th to 26th, engage with leading experts and discover cutting-edge methods for environmental and food analysis.</p><h5><strong>Why attend?</strong></h5><ul><li><strong>Comprehensive Learning:</strong> Dive deep into advanced LC-MS techniques and gain practical knowledge to enhance your laboratory's analytical capabilities.</li><li><strong>Expert Insights:</strong> Learn from industry leaders and gain firsthand experience through detailed modules on chromatography, mass spectrometry, and sample preparation.</li><li><strong>Regulatory Compliance:</strong> Stay ahead with the latest insights on EU regulations for PFAS analysis in food and water, ensuring your lab meets stringent standards.</li></ul><h3><strong>Episode #2: Tandem Quadrupole MS and MS-Method Development | F&amp;E Summer School - Day 1 - Module 2</strong></h3><p>In this episode it’s all about the tandem quadrupole mass specs and the setup of MS-methods. We will demonstrate, using actual hardware, how simple it is to prevent contamination or clean a system, regardless of whether it’s at the low or high end of the sensitivity spectrum. You will learn valuable tips and tricks about UniSpray, a unique ion source and alternative to ESI.</p><p>With our new waters_connect for Quantitation software, we will guide you through the different stages of MS-method development. You will learn how straightforward automated compound optimization can be, and how intuitively the new Method Acquisition Editor can be utilized by operators providing a clear structured all in one view of compounds and parameters. Search options make it easy to navigate through large multi-residue methods. And in the end, you are just one click away from the processing method that will be the topic of Episode 4.</p><h5><strong>What you'll learn:</strong></h5><ul><li>Easy operation and maintenance of Waters TQ-MS systems</li><li>Usage and benefits of UniSpray ion source</li><li>Step by step MS-method development with waters_connect for Quantitation</li></ul><blockquote><p><strong>Presenter:&nbsp;</strong>Benjamin Wuyts, PhD (Senior Solution Support Specialist Europe, Waters Corporation)</p><p>Benjamin initially joined Waters as an application chemist working in the Belgian demo lab, performing liquid chromatography-mass spectrometry demonstrations, support and training. In 2016 Benjamin became a European application chemist focusing on projects to support emerging applications. Benjamin has been focused over several years in establishing the Waters solution to the analysis of polar anionic pesticides, utilising novel column phases and LC-MS/MS technologies.</p></blockquote>

Tandem Quadrupole MS and MS-Method Development

In this episode it’s all about the tandem quadrupole mass specs and the setup of MS-methods.

<p><strong>PFAS testing has become one of the most important topics facing food and environmental laboratories over the last two years.</strong>&nbsp;</p><p>Phenomenex and SCIEX have worked with collaborators to overcome the significant challenges of such analyses, and now offer extremely sensitive and robust LC-MS/MS and sample preparation solutions, with methods and software which are ready to implement.</p><h4>Key Learning Points</h4><ul><li><strong>Background to PFAS and their regulations</strong></li><li><strong>System Settings and requirements</strong></li><li><strong>Correct HPLC column choice</strong></li><li><strong>Sample Preparation strategies from various matrices</strong></li><li><strong>PFAS as ubiquitous contaminants - Inerting the system</strong></li><li><strong>A sensitive and robust method applicable to water and food sample</strong></li><li><strong>Some insight about designed for PFAS products</strong></li></ul><h4>Who should attend</h4><ul><li>LC/MS or Sample Prep Users, working with several matrices that want to get tips on PFAS method development or on improving their method.</li></ul><blockquote><p><strong>Presenter: </strong>Jianru Stahl-Zeng, Dr.&nbsp; (Senior Technical Marketing Manager EMEAI, SCIEX)</p><p>Jianru has been part of SCIEX since 2001. She initially was part of our application Support team and covered not only proteomics but also small molecules applications. Jianru moved to technical marketing and focused on Food and Environmental applications.</p><p>Since 2018, she is managing the EMEAI technical marketing team and projects. Jianru is also the Global Technical Leader for Food and &nbsp;Environmental application.</p><p><strong>Presenter:&nbsp;</strong>Duilio Romanello (Senior Technical Specialist, Phenomenex)</p><p>Duilio has been working with Phenomenex since 2008. Since 2010 he has been Product Specialist for the GC and SPE lines and then Account Manager for the South of Italy. Since November 2023 he is covering the role of Senior Technical Specialist.</p><p>He has acquired experience in the Food and Environmental sectors by collaborating with important companies in the industry and providing technical support for the development of method optimization, guidance on applications and troubleshooting, assistance in choosing columns for HPLC, Gas Chromatography (GC), SFC and SPE.</p></blockquote>

Phenomenex: Advanced strategies in LC/MS/MS for the clean-up and the analysis of per- and polyfluoroalkyl substances (PFAS) in various matrices

Advanced strategies in LC/MS/MS for the clean-up and the analysis of per- and polyfluoroalkyl substances (PFAS) in various matrices

Extremely sensitive and robust LC-MS/MS and sample preparation solutions, with methods and software which are ready to implement.

Advanced strategies in LC/MS/MS for the clean-up

<h4><strong>Overview</strong></h4><p><strong>We invite you to our 2nd Annual Virtual Biosymposium. This year, we will explore novel analytical characterization and quantitation of emerging modalities, such as DNA and mRNA, along with key method development considerations with global thought leaders.</strong></p><p>We look forward to seeing you!</p><p><br><strong>Key Event Objectives:</strong></p><ul><li>Learn about novel approaches to characterizing established and modern biotherapeutic modalities</li><li>Understand key considerations when developing characterizing and quantitating methods for biomolecules</li><li>Take a deep dive into the values of fit-for-purpose methods to make critical drug decisions</li></ul><p><strong>AGENDA</strong></p><p><strong>7:30am AM (PT) - Welcoming Introductions</strong></p><ul><li>Tran N. Pham, Global Biopharma Market Development Manager at Phenomenex</li></ul><p><strong>7:35 AM (PT) - "Solutions &amp; Characterization Strategies for IVT mRNA Therapy Development"</strong></p><ul><li>Charles Bai, Lead Application Scientist, VectorBuilder, Chicago, USA</li></ul><p><strong>8:05 AM (PT) - &nbsp;"Utilizing Size Exclusion Chromatography for the Quality Control Analysis of Gene Therapeutics”</strong></p><ul><li>Rebecca Hirschberger, QC Manager Bioanalytical Implementation, and Dr. Christopher Gawlig, QC Manager Research and development, Biospring, Germany</li></ul><p><strong>8:35 AM (PT) "Impurity Profiling Optimization of GalNAc-conjugated Oligonucleotides by LC-MS-UV"</strong></p><ul><li>Roxana Eggleston-Rangel, Senior Applications Scientist, Phenomenex, California, USA</li></ul><p><strong>9:05 AM (PT) – Round Table Q&amp;A</strong></p><ul><li>Tran N. Pham, Global Biopharma Market Development Manager at Phenomenex</li></ul><p><strong>9:25 AM (PT) – Closing Remarks</strong></p><blockquote><p><strong>Presenter: </strong>Charles Bai (Lead Application Scientist)</p><p>Charles has received his PhD in Microbiology at Purdue University. As a Lead Application Scientist at VectorBuilder, his main responsibilities are to provide technical consultation for clients regarding gene delivery using viral and non-viral vectors. Regarding the application of in vitro transcribed (IVT) RNA, Charles has been helping researchers with the design, production and encapsulation of IVT mRNA, circRNA or saRNA for therapy and vaccine development.</p><p><strong>Presenter:</strong> Rebecca Hirschberger (Quality Control Bioanalytical Method Development)</p><p>Rebecca has been in the chromatography industry for over 2 years and has practical experience in the quality control of gene therapeutics. She has earnt a Masters in pharmaceutical science from Ludwig-Maximilians University, Munich, focused on instrumental analytics. Since Dezember 2022 she is a licensed Pharmacist in Germany. Rebecca Hirschberger joined BioSpring in August of 2021 and she serves as QC Manager Bioanalytical Implementation. Her area of responsibility includes: Various analyses of oligonucleotides, including (tandem) mass spectrometry via qToF, biochemical techniques, i.e. ELISA, enzymatic transformation of oligonucleotides for analytical and quality control purposes and the Separation of oligonucleotides by chromatographic methods.</p><p><strong>Presenter: </strong>Roxana Eggleston-Rangel (Senior Applications Scientist, Phenomenex)</p><p>Roxana joined Phenomenex in 2018 as an Applications Scientist and is currently working with the applications team to produce analytical solutions for scientist in the biopharma industry. Roxana’s current work focuses on characterization of mRNA, sgRNA and siRNA. Prior to Phenomenex, Roxana worked for Caltech and the University of Southern California developing LM-CS workflows for the proteomics community. Roxana Eggleston-Rangel received her bachelor’s and master’s degree in Chemistry and Biochemistry from California State University, Los Angeles.</p></blockquote>

Phenomenex: 2nd Annual Virtual Biosymposium

2nd Annual Virtual Biosymposium

Explore novel analytical characterization and quantitation of emerging modalities, such as DNA and mRNA

Advanced strategies in LC/MS/MS for the clean-up and the analysis of per- and polyfluoroalkyl substances (PFAS) in various matrices

Tandem Quadrupole MS and MS-Method Development

Stationary Phases in HPLC - Part II

Chromatography - UPLC

PFAS in Food Supply Chains: Regulations and Analytical Approaches: in association with AOAC India Section

Automated Data Management for LC/GC Workflows

Comprehensive chromatographic assessment of forced degraded in vitro transcribed mRNA

Nitrosamines & NDSRI's: Evolving Regulatory Landscape in Nitrosamines and NDSRI’s analysis.

Lipid nanoparticles

2nd Annual Virtual Biosymposium

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