Ultrashort chain per- and polyfluoroalkyl substances (USC-PFAS),defined as those with three or fewer carbon atoms, are characterisedby their significant mobility within the environment, high water solubility,and low affinity for organic matter. These properties contributeto their widespread presence in environmental waters and wastewaters,as well as drinking waters. The environmental concerns associatedwith USC-PFAS include their persistence, potential for groundwatercontamination, and challenges in remediation due to their resistance to conventional treatment methods.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_2_093d51b263.jpg" alt="EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources: Figure 1: Illustrative picture" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_2_093d51b263.jpg 245w," sizes="100vw" width="602" height="247"><h4>Occurence, uses and sources</h4>Ultrashort chain PFAS have been poorly characterised compared with their longer chain counterparts, and understanding of their intended and unintended transformation processes is limited. Sources of USCPFAS include degradation of precursor compounds, atmospheric degradation of hydrofluorocarbons and hydrochlorofluorocarbons used as refrigerants, and intentional direct use in products like batteries. Crucially, TFA, a major ultrashort-chain PFAS, is also formed through the environmental breakdown of fluorinated pesticides and pharmaceuticals. They are also byproducts from historical electrochemical fluorination (ECF) manufacture of longer chain PFAS. USC-PFAS are also found in urban and industrial waste, as well as aqueous film-forming foams (AFFFs) used in firefighting.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_5698c634d6.jpg" alt="EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources: Figure 2: PFSA and PFCA categorization based on carbon chain length – ultrashort-chain, short-chain and long-chain." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_Czech_Republic_Enviromail_29_Unstoppable_Ultrashort_PFAS_in_the_environment_and_water_sources_5698c634d6.jpg 245w," sizes="100vw" width="1496" height="629"><h4>Fate and transport</h4>Carbon chain length plays a key role in determining the physicochemical characteristics of different PFAS chemicals. Long chain PFAS for instance are more hydrophobic, a property that has been leveraged by removal techniques using carbonaceous materials like granular activated carbon (GAC). By contrast, short and ultrashort chain PFAS are hydrophilic, with characteristically low pKa values (i.e. more acidic), high water solubilities and decreased log Koc values (i.e. less adsorptive to organic carbon). These attributes mean that USC-PFAS dissociate freely in aqueous environments and are less prone to sorption onto natural solids. The outcome is that USCPFAS are highly mobile in the aquatic environment and can travel far from contamination source areas.<h4>The global regulatory landscape</h4>Awareness about the persistence and bioaccumulative nature of C8-C14 PFAS began to attract widespread concern near the turn of the millennium, and inclusion of PFOS, PFOA and PFHxS on the persistent organic pollutants (POPs) list of the Stockholm Convention soon ensued. Consequentially, manufacturers began adopting short and ultrashort chain alternatives.However, with the change from C8 to C4 and shorter fluorinated carbon chains came the sacrifice to lower technical performance, and greater quantities were needed for equivalent performance. Hence, while it is generally accepted that short and ultrashort chain PFAS have lower toxicological profiles, environmental risks are not necessarily obviated since USC-PFAS presence, and continuous, cumulative, release suggests that adverse risks may lie ahead.Global regulations of USC-PFAS vary by region and are often included under broader PFAS regulations. For example, the European Union has specific guidelines and limits for the total concentration of PFAS compounds in drinking water (as set out in EU Directive 2020/2184). The EU's Drinking Water Directive established a limit of 0.5 μg/L for 'PFAS Total,' although ongoing discussions concern the specific methodology for incorporating trifluoroacetic acid (TFA). Furthermore, TFA was recently added to the priority list for the initial proposed sum of 24 PFAS in surface water. <a target="_blank" rel="noopener noreferrer" href="https://www.consilium.europa.eu/en/press/press-releases/2025/09/23/water-pollution-council-and-parliament-reach-provisional-deal-to-update-priority-substances-in-surface-and-ground-waters/">LINK</a><h4>PFAS sampling containers</h4>Only tested and verified sampling containers are recommended to ensure reliable results in PFAS analysis; these containers are available upon request from ALS laboratories.<h4>Targeted analytical services are becoming more accessible</h4>Testing for USC-PFAS is challenging due to several analytical factors. Background contamination and matrix noise can interfere with the detection of these compounds, particularly trifluoroacetic acid (TFA). Furthermore, sample preparation presents a significant challenge because of the constant risk of cross-contamination. To mitigate this risk, the sample preparation procedure was specifically designed to be as simple and straightforward as possible.Other USC-PFAS compounds generally have lower ambient background concentrations, which makes achieving lower Limits of Reporting (LOR) more readily achievable. ALS has successfully developed a testing method that includes USC-PFAS compounds in environmental water matrices, achieving a total LOR of 50 ng/L for TFA and even lower LORs for other USC compounds. The full list of analyzed USC-PFAS is summarized in table 1:<h5>USC-PFAS Compounds / CAS No.</h5><ul><li>Trifluoroacetic acid (TFA), Perfluoroethanoic acid (PFEtA): 76-05-1</li><li>Trifluoromethane sulfonic acid (TFMS), Perfluoromethane sulfonic acid (PFMeS): 1493-13-6</li><li>Perfluoroethane sulfonic acid (PFEtS): 354-88-1</li><li>Perfluoropropanoic acid (PFPrA): 422-64-0</li><li>Perfluoropropane sulfonic acid (PFPrS): 423-41-6</li><li>Trifluoromethane sulfinic acid: 34642-42-7</li><li>Lithium bis(trifluoromethanesulfonyl)imide (LiTFSI): 90076-65-6</li></ul>&nbsp;

EnviroMail™29/Europe: Unstoppable? Ultrashort PFAS in the environment and water sources

<h4>Why Consider HILIC in Modern Oligonucleotide Analysis?</h4>Oligonucleotides play a central role in gene therapies, molecular diagnostics, and cutting-edge biomedical research. Before these molecules reach application, however, they must be carefully characterized to confirm purity, identity, and performance.Analyzing therapeutic oligonucleotides is far from straightforward. Their polarity, structural diversity, and modifications demand highly selective and MS-compatible separation methods. Although ion-pair reversed-phase LC (IP-RP-LC) remains the established standard for LC-MS workflows, hydrophilic interaction liquid chromatography (HILIC) is rapidly emerging as a powerful complementary technique.When IP-RP approaches reach practical or performance limits, HILIC offers an attractive alternative, delivering strong retention and efficient separation of highly polar oligonucleotides — without relying on ion-pairing reagents.<h4>How Does HILIC Separate Oligonucleotides?</h4>HILIC essentially reverses conventional reversed-phase logic. It combines:<ul><li>A polar stationary phase</li><li>An organic-rich mobile phase</li></ul>In this configuration, the stationary phase retains a thin, water-enriched layer on its surface. Polar analytes — such as oligonucleotides — partition into this aqueous layer while the acetonitrile-rich mobile phase passes through.This mechanism allows:<ul><li>Retention of short or highly polar oligonucleotides</li><li>Separation of modified sequences</li><li>LC-MS compatibility without ion-pairing reagents</li></ul>The key to performance lies in carefully tuning the parameters that govern HILIC selectivity and retention.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Key_Parameter_of_HILIC_Mode_fb364c9a8b.webp" alt="KNAUER: Figure 1 - Key Parameter of HILIC Mode." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Key_Parameter_of_HILIC_Mode_fb364c9a8b.webp 245w," sizes="100vw" width="945" height="433"><h4>What Controls Retention in HILIC?</h4>Several interaction mechanisms contribute to analyte behavior in HILIC:<h5>Hydrophilic Partitioning – The Primary Mechanism</h5>Polar molecules distribute into the thin water layer formed on the stationary phase surface. The greater the polarity of the analyte, the stronger its affinity for this layer, leading to increased retention.<h5>Hydrogen Bonding – Fine-Tuning Effects</h5>Functional groups such as –OH, –NH₂, and –COOH can form hydrogen bonds with the stationary phase, subtly influencing retention time and selectivity.<h5>Electrostatic Contributions – The Role of Charge</h5>Some HILIC materials (e.g., bare silica) exhibit surface charges. Depending on mobile phase pH and buffer composition, electrostatic interactions can either enhance or suppress analyte retention.<h5>Dipole and Ion–Dipole Interactions – Added Selectivity</h5>Highly polar or zwitterionic compounds — including phosphorylated species and sugars — may engage in dipole–dipole or ion–dipole interactions, further refining separation behavior.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hydrophilic_Partitioning_on_Water_enriched_Layer_41ab9ac902.webp" alt="KNAUER: Figure 2 - Hydrophilic Partitioning on Water-enriched Layer." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hydrophilic_Partitioning_on_Water_enriched_Layer_41ab9ac902.webp 245w," sizes="100vw" width="945" height="409"><h4>How to Develop a HILIC Method for Oligonucleotides</h4>Like any chromatographic method, successful HILIC development follows a structured approach:<h5>Step 1: Define the Analytes</h5>Understand oligonucleotide length, polarity, charge, and modifications.<h5>Step 2: Select a Suitable Column</h5>Choose a polar stationary phase (e.g., amide or zwitterionic) appropriate for the oligonucleotide’s chemical properties.<h5>Step 3: Optimize the Mobile Phase</h5><ul><li>Start with 70–95% acetonitrile</li><li>Use a volatile buffer (e.g., ammonium formate or ammonium acetate, typically 5–20 mM)</li><li>Adjust pH (commonly between 6–8, with testing up to pH 9 if column stability allows)</li></ul>Small pH shifts can significantly alter selectivity and MS response.<h5>Step 4: Design the Gradient</h5>Employ a gradient that increases water content to modulate retention and separate oligonucleotides by size or chemical modification.<h5>Step 5: Match Injection Conditions</h5>Samples should be injected in a solvent similar to the starting mobile phase (high organic content) to prevent peak distortion.<h5>Step 6: Evaluate Performance</h5>Assess:<ul><li>Resolution</li><li>Retention time stability</li><li>Peak shape</li><li>Reproducibility</li></ul>Balancing separation performance with MS ionization efficiency is essential.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_HILIC_Method_Step_Procedure_for_Oligos_f3b9ea6b08.webp" alt="KNAUER: Table 1 - HILIC Method Step Procedure for Oligos." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_HILIC_Method_Step_Procedure_for_Oligos_f3b9ea6b08.webp 245w," sizes="100vw" width="945" height="357"><h4>Practical Tips for High-Performance HILIC</h4><h5>Maintain a Stable Water Layer</h5>A minimum of ~5% water is necessary at the start of the run. Too little water can eliminate retention entirely. Adjust water content carefully to fine-tune retention.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Water_Adjustment_Principle_for_HILIC_c0d8616f20.webp" alt="KNAUER: Figure 3 - Water Adjustment Principle for HILIC." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Water_Adjustment_Principle_for_HILIC_c0d8616f20.webp 245w," sizes="100vw" width="951" height="374"><h5>Use High Organic Content</h5>HILIC typically operates best with 70–95% acetonitrile. Polar analytes are retained more effectively under these conditions.<h5>Include a Volatile Buffer</h5>Use low concentrations (5–20 mM) of ammonium formate or acetate to:<ul><li>Stabilize pH</li><li>Reduce peak tailing</li><li>Maintain MS compatibility</li></ul><h5>Control Injection Solvent</h5>Avoid high-water sample solvents. They disrupt the partitioning mechanism and distort peaks.<h5>Allow Proper Column Equilibration</h5>HILIC columns require longer equilibration times than reversed-phase columns — typically 10–20 column volumes after major gradient or solvent changes.<h5>Consider Temperature</h5>Operating at 30–50 °C can:<ul><li>Improve peak shape</li><li>Reduce mobile phase viscosity</li><li>Stabilize retention behavior</li></ul><h4>From Alternative to Essential? Final Perspective</h4>HILIC-MS is steadily advancing as a strong, ion-pair-free option for oligonucleotide analysis. While IP-RP-LC still dominates in sensitivity for many applications, HILIC is closing the gap and offering valuable flexibility — particularly for highly polar or challenging sequences.Although HILIC follows different chromatographic principles than reversed-phase separations, once optimized, it can deliver robust and highly selective results.Rather than being a niche method, HILIC is becoming a strategic tool in modern oligonucleotide workflows — especially when MS compatibility and method versatility are priorities.If you are developing or refining an oligonucleotide LC-MS method, HILIC may be worth integrating into your analytical toolbox.<blockquote><ul><li>For additional information or discussion, feel free to contact: <a>sales@knauer.net<svg><use></use></svg></a></li><li>For direct scientific inquiries, reach out to the author at: <a>kindler@knauer.net<svg><use></use></svg></a></li></ul></blockquote>Stay tuned for more insights in the “Oligos Made Easy” series.

Oligos Made Easy - Part 4: HILIC

News from LabRulezLCMS Library - Week 09, 2026

Article

Application

Academy

Environment

Science and research

Poster

For more than 40 years, ALS has provided testing and technical solutions to clients all over the world in a wide range of industries. We have built our global reputation by embracing the latest technologies and innovative methodologies to deliver high quality testing services to clients. ALS is able to leverage a multifaceted international team while remaining agile enough to provide local expertise and personalized solutions to every one of our clients.

ALS Czech Republic

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Biotage

Agilent Technologies

Thermo Fisher Scientific

SelectScience

Shimadzu Corporation

Waters Corporation

<h4>Agilent Webinar Series for Food Testing Workflows: Part 7</h4>
Nowadays, laboratories have the challenge of becoming progressively more productive as well as focusing on continuous improvements. 
Food laboratories such as contract labs, government entities and private companies are therefore adopting practices to have faster sample turnaround and to reduce the solvent consumption per run. 
During this presentation, we will examine approaches to address these aspects. We will also discuss the use of latest technology with examples to illustrate how to better achieve the desired outcomes.
<blockquote>
Presenter: Cecilia Mazza, (Product Specialist, EMEA IDO - Chemistries &amp; Suppliers, Agilent)
</blockquote>
<blockquote>
With more than 20 years of professional experience, Cecilia is presently the Product Specialist for columns and supplies at Agilent. Supporting distributors and laboratory users in the analysis and purification of small and large molecules, for a wide range of LC and GC applications as well as sample preparation.
</blockquote>
<blockquote>
Cecilia holds a Ph.D. in Chemical Engineering from Rensselaer Polytechnic Institute in US.
</blockquote>

Agilent: Agilent Webinar Series for Food Testing Workflows: Better Lab Sample Turnaround - Saving Time and Solvent per run

Better Lab Sample Turnaround - Saving Time and Solvent per run

Approaches to faster sample turnaround and to reduce the solvent consumption per run.

Better Lab Sample Turnaround - Saving Time and Solvent

With the goal towards net zero emissions by 2050, the global urgency to find cleaner energy alternatives has never been more pressing, although the world is only at its initial stages of transitioning away from fossil fuels.
In the push for sustainable renewable energy, bioenergy stands out as a promising solution, demonstrated by its status as the largest source of renewable energy worldwide. Biofuels, derived from biomass, uniquely distinguish themselves as the only renewable energy source that can be directly used in the transport sector. This capability allows biofuels to overcome traditional barriers, enter the market quickly, and drive accelerated change towards clean energy. Biofuels hold significant potential to decarbonize hard-to-electrify sectors, such as aviation and shipping.
While 1st generation biofuels raise concerns about compromising food security in the food vs. fuel debate, advanced biofuels derived from non-food sources—including 2nd generation biofuels from agricultural waste and woody biomass, and 3rd generation biofuels from algae biomass—present a viable path to fuel a sustainable future.
The session will deliver a comprehensive guide to the analysis of various biofuels, including biogas, biodiesel, and bioethanol, providing valuable insights that you need to understand this much discussed topic.
<h5>Learning Objectives:</h5>
<ul>
<li>Understand the importance of biofuels as a sustainable energy source and their potential impact on reducing greenhouse gas emissions and dependence on fossil fuels
</li>
<li>Learn about the different types of biofuels, their production processes, and the challenges associated with their analysis and quality control
</li>
<li>Understand the importance of quality control and assurance in biofuel production, including sampling techniques, calibration, and validation of analytical methods
</li>
<li>Develop an understanding of the role of biofuel analysis in supporting the transition to a sustainable future and the contribution it can make towards achieving global energy and environmental goals
</li>
</ul>
<blockquote>
Presenter: Jackie, PhD (Assistant Product Manager (GC &amp; GCMS), Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Jackie obtained his PhD in Analytical Chemistry from the National University of Singapore, He has garnered over 10 years of research, training, and facilitation experience. Jackie meets scientists from various industries to share technical knowledge and collect feedback, understanding their problems and helping them find well-suited solutions in GC-related analyses.
</blockquote>
<blockquote>
Presenter: Hashi Yuki, PhD, (Senior Expert, Shimadzu Corporation)
</blockquote>
<blockquote>
Dr. Hashi, a graduate of Osaka University, Japan, joined Shimadzu Corporation in 1986. With expertise in HPLC and protein analysis, he served as an application manager for chromatography instruments in Southeast and South Asia. In China, he became the general manager for chromatographic instrument development and support. Dr. Hashi holds a Ph.D. from The Chinese Academy of Sciences and has lectured at various universities. Currently, he works with Shimadzu Corporation in Japan, focusing on developing the petroleum and petrochemical market.
</blockquote>
<blockquote>
Presenter: Nagao Yu (Assistant Manager (Green Energy), Shimadzu Corporation)
</blockquote>
<blockquote>
Mr. Nagao graduated from the Department of Chemistry, Graduate School of Science, Nagoya University in 2016. In the same year, he joined Shimadzu Corporation and engaged in application development using GC and GCMS. Since 2022, he has been working in the marketing department of the new energy field.
</blockquote>

Shimadzu: Fuelling a Sustainable Future: Solutions For Biofuels Analysis

Fuelling a Sustainable Future: Solutions For Biofuels Analysis

Comprehensive guide to the analysis of various biofuels, including biogas, biodiesel, and bioethanol, to understand this much discussed topic.

Fuelling a Sustainable Future: Biofuels Analysis

Attendees will see how currently available technologies can provide major solvent (and time) savings and improve their workflow and productivity.
<h5>This presentation provides easy to do suggestions to reduce organic solvent use including:</h5>
<ul>
<li>Step gradients</li>
<li>Smaller particle size columns</li>
<li>HILIC as replacement for DCM/MeOH</li>
<li>Reversed phase</li>
</ul>
<h5>Who should attend this webinar mini-series?</h5>
<ul>
<li>Small Molecule Organic Chemists</li>
<li>Medicinal Chemists</li>
<li>And their Managers</li>
</ul>
<blockquote>
Presenter: Bob Bickler (Senior Technical Specialist, Biotage)
</blockquote>
<blockquote>
Bob Bickler is the Senior Technical Specialist at Biotage and has over 45 years of chromatography experience. During his career, he has worked for Whatman, YMC, Waters, and for the past 25 years at Biotage. Bob has designed and launched many products including Biotage SNAP columns, SP1, Isolera LS, Isolera Prime Flash System, as well as several accessories.
</blockquote>

Biotage: Improve your Flash Chromatography Skills: Tips for Sustainable Purifications

Improve your Flash Chromatography Skills: Tips for Sustainable Purifications

Attendees will see how currently available technologies can provide major solvent (and time) savings and improve their workflow and productivity.

Better Flash Chromatography Skills: Tips for Purifications

<h4>New innovation for daily excellence in ion chromatography: consistent results across operators and shifts!</h4>
Are you regularly performing ion chromatography tests? 
We&#39;ve listened to the feedback given by ion chromatography (IC) users and created a top-notch IC system to enable you to generate reliable and constant IC results – all day, every day, no matter who&#39;s running the tests. 
Detlef Jensen, our European senior expert on ion chromatography, will present our innovative new IC system designed to support laboratories in routinely generating dependable and consistent IC results. This new system will reduce IC training requirements and system downtime, and you can easily upgrade the system to meet ever-changing laboratory needs.
<h5>Why Should You Attend?</h5>
<ul>
<li>Get an in-depth look at our latest cutting-edge IC platform.</li>
<li>Learn how this IC platform can stretch costs further and give you flexibility for evolving business needs.</li>
<li>Discover how you can routinely acquire repeatable and reliable results.</li>
</ul>
<h5>This webinar is for scientists and laboratory professionals:</h5>
<ul>
<li>Working with water or wastewater analysis </li>
<li>Analysing APIs or impurities in pharmaceutical mixtures </li>
<li>Testing food samples for ionic substances</li>
</ul>
Join us on February 15th to explore the opportunities and advancements this IC system can bring to your laboratory. As a bonus, get tips on how to optimize your IC results, too. 
Can&#39;t attend the live broadcast? Np problem! Register anyway and you will automatically recive the link to the webinar recording.
<blockquote>
Presenter: Detlef Jensen (Sr. Application Expert Applied Markets, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Detlef holds a PhD in chemistry from the Technical University Berlin/Germany. For 30 years, he covered many positions at Dionex and Thermo Fisher Scientific. He is supporting our users and our team of field experts all over Europe with his deep knowledge of all aspects of ion chromatography and its applications.
</blockquote>

ThermoFisher Scientific: Ion chromatography innovation: New platform for routine IC applications

Ion chromatography innovation: New platform for routine IC applications

Innovative IC system designed to support laboratories in routinely generating dependable and consistent IC results will presented.

New platform for routine IC applications

<h3>Don&#39;t miss our one-day refurbished instruments sale</h3>
This is your exclusive invitation to Agilent’s pre-loved instruments Valentine Cyber Sale.
Join this event and immerse yourself in a dynamic online experience. Find chemistry you can trust, in our extensive refurbished analytical instrument portfolio, including chromatography, spectroscopy, mass spectrometry and automation.
During this event, our interactive platform enables you to:
<ul>
<li>Explore our inventory of refurbished instruments and their irresistible prices for this event only.</li>
<li>Gain insight into Agilent&#39;s meticulous refurbishment process. </li>
<li>Access informative brochures, engaging videos, and detailed instrument specifications.</li>
<li>Engage in live Q&amp;A sessions to get all your queries answered.</li>
<li>Secure the option to purchase the instrument of your choice.</li>
</ul>
Don&#39;t miss this opportunity to join us and find a relationship guaranteed to last at least 10 years!
<blockquote>
<h4>Agilent Certified Pre‑Owned Instrument program</h4>
</blockquote>
<blockquote>
Agilent Certified Pre‑Owned Instrument program is a source of pride throughout our company. An array of instruments are available and there are plenty of things to love:
</blockquote>
<blockquote>
<ul>
<li>Agilent Uncompromising analytical capabilities</li>
</ul>
</blockquote>
<blockquote>
<ul>
<li>Agilent Meet your sustainability goals</li>
</ul>
</blockquote>
<blockquote>
<ul>
<li>Agilent Prices that won’t break your heart</li>
</ul>
</blockquote>

Agilent: Find Your Perfect Match at Agilent’s Valentine Cyber Sale

Find Your Perfect Match at Agilent’s Valentine Cyber Sale

Refurbished analytical instrument portfolio, including chromatography, spectroscopy, mass spectrometry and automation.

Minimize risk, streamline workflows and improve the reliability of your analytical measurements.
How do the Alliance iS and Empower work together to reduce common human errors?
Empower 3.8.0 offers unique features that work specifically with Waters Alliance iS HPLC System and can reduce common made human errors.
Learn how to identify potential errors earlier, reduce the number of retests and failure investigations and register!
<blockquote>
Presenter: Arjan Timmerman (Principal Pharma Market Development Manager Pharmaceuticals, Compliance and Impurities)
</blockquote>
<blockquote>
Presenter: Andrea Gheduzzi (Principal LC Market Development Manager, EMEA)
</blockquote>
<blockquote>
Presenter: Carsten Fischer (Informatics Product Marketing, EMEA)
</blockquote>

Waters Corporation: Reducing Human Errors with Empower 3.8.0

Reducing Human Errors with Empower 3.8.0

Empower 3.8.0 offers unique features that work specifically with Waters Alliance iS HPLC System and can reduce common made human errors.

<h3>Biopharma Brilliance Webinar Series:</h3>
 Glycan &amp; Sialic Acid Workflows in Biotherapeutic Glycoproteins: Sample Prep to Analysis.
<h4>Session 1: Workflows for Released N-Glycans: Sample Preparation and Analysis</h4>
Glycosylation is considered one of the critical quality attributes of biomolecules. Therefore, there is a need to characterize, quantify, and determine if they are in acceptable ranges as part of the development process for a glycoprotein innovator, biosimilar or bio-better pharmaceutical.
In the race to discover the next promising biotherapeutic or develop a reliable biosimilar, one cannot compromise on analytical accuracy and efficiency. Reducing process development time, having the ability to make procedural changes quickly, and minimizing variability in analyses are just a few of the challenges faced every day.
 Join us for an engaging webinar to learn about these challenges and how to try to overcome them.
What will you learn?
<ul>
<li>Efficient preparation and analysis of released N-glycans from biotherapeutic glycoproteins.</li>
<li>Techniques to significantly reduce sample preparation time and enhance N-glycan analysis productivity.</li>
<li>Selection criteria for an appropriate HILIC column and mobile phase.</li>
<li>Achieving greater separation of charge groups with the new Agilent AdvanceBio Amide HILIC column.</li>
</ul>
Join both the session and get certified for Brilliance.
<blockquote>
Presenter: Dr. Rajni Mujoo (Technical Support Specialist, CSD, Agilent Technologies)
</blockquote>
<blockquote>
Dr. Rajni is working with us as Technical Support Specialist in Chemistries and Supplies Division at Agilent Technologies since 2011. She has a Ph.D degree in Food Science from CFTRI, Mysore and Post Doctorate from Department Of Food Science, Michigan State University. She also has four International Presentations &amp; 13 publications in reputed National &amp; International journals to her credit. Rajni has a total work experience of 16 yrs spread across research and Sales building expertise in different Instrument techniques.
</blockquote>
<blockquote>
Presenter: Mr. Shreyas Tribhuvane (Business Development Manager, PL BC, Agilent Technologies)
</blockquote>
<blockquote>
Shreyas has 10 years experience in working closely with biopharma customers in understanding their analytical characterization needs. As a BDM for Biocolumns and Glycan kits his prime responsibility is to work on the Biopharma market needs and communicate with Agilent solutions unit team in order to have apt solutions. He has a hands-on experience on Agilent Glycan sample prep kits and helped customers in setting up these kits for their glycan characterization work.
</blockquote>

Agilent: Biopharma Brilliance - Session 1

Biopharma Brilliance - Session 1

Process development time, procedural changes, and variability in analyses are just a few of the challenges faced every day.

Parallel capillary electrophoresis is a powerful technique for automated protein analysis. The ProteoAnalyzer system is a novel instrument that uses this technique to overcome the limitations of traditional methods. It features 12 capillaries, validated reagents, and automated cleaning and rejuvenation after each run, enabling the user to obtain high-quality data for various protein sample types.
In this webinar, Dr. Kyle Luttgeharm, Product Manager and Dr. Gizem Bergdahl, Research Scientist, DAKO Denmark R&amp;D &amp; Manufacturing, will discuss how the ProteoAnalyzer system can transform protein analysis workflows.
Key learning objectives
<ul>
<li>Understand more about the ProteoAnalyzer</li>
<li>Learn how to improve the consistency of your protein analysis results</li>
<li>Explore how the ProteoAnalyzer can be used to analyze challenging sample types including total protein extracts and crude cell lysates</li>
</ul>
Who should attend?
<ul>
<li>Anyone who needs help to gain laboratory efficiency, free up technician time, and minimize delays due to ambiguous results leading to re-sample analysis.</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Dr. Kyle Luttgeharm (Product Manager, Agilent Technologies)
</blockquote>
<blockquote>
Dr. Kyle Luttgeharm is a Product Manager with Agilent Technologies. He holds undergraduate degrees in Biology and Biochemistry from Southwestern College in Winfield, Kansas, and a doctorate in Biochemistry from the University of Nebraska-Lincoln. Dr. Luttgeharm previously worked as an Application Scientist with Advanced Analytical Technologies, Inc. and as an R&amp;D Scientist with Agilent Technologies. He has extensive experience developing methodologies and assays for Agilent’s parallel capillary electrophoresis technology.
</blockquote>
<blockquote>
Presenter: Dr. Gizem Bergdahl (Research Scientist, DAKO Denmark R&amp;D &amp; Manufacturing, Agilent Technologies, (Glostrup, Denmark)
</blockquote>
<blockquote>
Dr. Gizem Bergdahl has a deep understanding of diagnostics, clinical biochemistry, human diseases, and in vitro diagnostics, and extensive experience in areas including immunoassays (ELISA, immunoturbidimetry, and chemiluminescence), ligand-binding assays (SPR), antibody conjugation to latex, and magnetic beads.
</blockquote>
<blockquote>
Presenter: Blake Forman (Scientific Content Creator, SelectScience)
</blockquote>
<blockquote>
Blake studied chemistry at the University of Surrey, UK, and further went on to study a master’s in chemistry at the University of Southampton, UK. Blake has previously worked in the Medicines and Healthcare Products Regulatory Agency (MHRA) lab at LGC and as a technical sales specialist for Waters. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.
</blockquote>

Agilent: Improve laboratory efficiency with automated protein analysis

Improve laboratory efficiency with automated protein analysis

In this webinar, will be discussed how the ProteoAnalyzer system can transform protein analysis workflows.

Improve lab efficiency with automated protein analysis

Liquid chromatography requires the selection and manipulation of several components to be an effective tool in separating complex mixtures. One of the most impactful components is the column stationary phase, which is where the separation occurs. However, a wide variety of column options are available regarding base particles, ligands and column hardware, making it very confusing which column to select for a specific analysis. A deeper dive into the important attributes of the column stationary phase is needed to fully understand how a column can impact the separation of analytes.
This webinar will be the first in a series and will focus on the different aspects of a column, ranging from base particle to column hardware selection. Chromatographic examples will be provided to highlight the different column attributes, such as pore diameter and base particle composition. Guidance will be given regarding column selection for method development. Understanding the basic attributes of the column stationary phase can better inform decisions when selecting columns, as well as allowing scientists to create the most robust methods possible. This translates into better and more reliable end results.
<blockquote>
Presenter: Kenneth Berthelette (Senior Scientist, Waters Corporation Chemistry and Consumables Group)
</blockquote>
<blockquote>
Ken earned his Bachelor&#39;s of Science degree in Professional Chemistry from Bridgewater State University, Bridgewater Massachusetts, in 2010. He has been with Waters Corporation since March 2012 and has worked on several product launches including CORTECS columns, UPC2 Torus and Trefoil columns, and the Atlantis Premier BEH Z-HILIC column. He changed job functions in 2021 and now supports Chemistry Aftermarket initiatives. His focus on applications and customer education has led to several seminars and webinars on topics including method development, method modernization and transfer, HILIC separation techniques, and LC system troubleshooting.
</blockquote>

Waters Corporation: Understanding LC Column Attributes: A Practical Guide to Column Selection

Understanding LC Column Attributes: A Practical Guide to Column Selection

This webinar will focus on the different aspects of a column, ranging from base particle to column hardware selection.

A Practical Guide to Column Selection

Regardless of your sample, it is critical to simplify your routine analysis work and infuse as much automation as possible into your workflow so you can increase confidence in your results and reduce the time it takes to make decisions. Empower Software offers you the flexibility to optimize operations in your lab.
To ensure that your method is performing as expected, routine food analysis calls for calibration/quantitation along with system suitability and/or control samples. These applications call for some commonly made calculations such as:
<ul>
<li>Percent Difference compared to a Target Amount</li>
<li>Average Amount in Duplicate Samples</li>
<li>Percent Deviation for Standards</li>
</ul>
Determining authenticity of botanical ingredients used in dietary supplements can be challenging. This routine test is simplified by automating evaluation of the following:
<ul>
<li>Chromatographic Pattern</li>
<li>Mass Spectral Pattern</li>
<li>Spectral Library Match</li>
</ul>
To further streamline, using tailored reports in Empower Software makes interpretation of results easy and decision-making faster with simple pass/fail, so you know whether you can ship a batch.
Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.
<blockquote>
Presenter: Neil Lander (Principal Product Manager, Informatics, Waters Corporation)
</blockquote>
<blockquote>
Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.
</blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Streamlining Efficiencies in Your Routine Analysis – Food and Dietary Supplement Applications