<div><p><strong>Purifying the target protein is crucial for investigating its specific properties and interactions with biomolecules like DNA, RNA, and other proteins. A range of purification strategies is available, and the optimal approach depends on factors such as the required scale, throughput, and downstream application. In many cases, the process must be fine-tuned through empirical optimization to achieve the desired purity and yield.</strong></p></div><div><div><div><div><h3><strong>What is Protein Purification?</strong></h3><p>Protein purification is a critical process in biotechnology and life sciences, aimed at isolating a specific protein from complex biological mixtures such as cell lysates or tissue extracts. The goal is to obtain a highly purified protein sample, free from contaminants like other proteins, nucleic acids, and lipids, to enable accurate analysis of its structure, function, and interactions.</p><p>This process is especially vital in biotechnological areas inside immunology and microbiology. In <strong>immunology</strong>, it underpins the production of vaccines, therapeutic antibodies, and diagnostic assays by enabling the isolation of key immune proteins and antibodies. It also supports the investigation of cell signaling and immune response mechanisms. In <strong>microbiology</strong>, purified proteins are essential for studying pathogens, identifying disease-related antigens, and driving drug discovery efforts. Ultimately, protein purification is fundamental to the development of targeted vaccines, innovative diagnostics, and effective therapeutic strategies.</p></div></div></div></div><div><div><div><div><h3><strong>Types of Protein Purification Methods</strong></h3></div></div></div></div><div><p>There are several types of protein purification methods, each chosen based on the protein’s characteristics such as size, charge, solubility, and binding affinity. Below are some commonly used methods:</p></div><div><div><div><div><h4><strong>Non-Chromatographic Approaches</strong></h4></div></div><div><p>Non-chromatographic approaches use simpler physicochemical principles—such as solubility, molecular size, or diffusional properties—to separate and purify proteins. These techniques are often employed as initial purification or concentration steps before further refinement.</p><h5><strong>Precipitation Techniques</strong></h5><p>Protein precipitation is a widely used approach in purification workflows, typically relying on one of three main methods:</p><ul><li><strong>Organic solvent precipitation</strong> involves adding solvents like ethanol or acetone to lower the solution's dielectric constant. This promotes protein–protein interactions, reducing solubility and leading to aggregation and precipitation.</li><li><strong>Isoelectric point precipitation </strong>exploits the pH at which a protein carries no net charge. At this point, solubility is minimized, causing the protein to precipitate from solution.</li><li><strong>Nonionic polymer precipitation</strong> uses polymers such as dextran, polyethylene glycol (PEG), or NPEP to alter the solution’s physical properties. These agents create crowding effects that promote protein aggregation without directly binding to the proteins.</li></ul><h5><strong>Dialysis</strong></h5><p>Dialysis involves placing a protein solution inside a semi-permeable membrane that allows water and ions to pass while the protein remains inside, facilitating buffer exchange.This method is commonly used during protein expression and purification workflows for buffer exchange, desalting, and removal of unwanted small molecules such as imidazole, urea, or ammonium sulfate. The process helps maintain protein stability and activity by gradually equilibrating the sample with the desired buffer conditions.</p><h5><strong>Immunocapture (Magnetic Bead-Based Purification</strong></h5><p>Immunocapture uses antibodies immobilized on solid supports like magnetic beads to selectively bind and isolate a target protein. This method is highly specific and particularly useful for low-abundance targets or small-scale prep, such as:</p><ul><li>Pulling down proteins from complex mixtures</li><li>Capturing tagged recombinant proteins (e.g., FLAG, HA, or His tags)</li><li>Enrichment before western blotting, MS, or ELISA</li></ul><p>Magnetic beads offer speed, ease of use, and minimal sample loss, making them ideal for lab-scale workflows.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/products/biozenspe-sample-preparation-products/biozen-magbeads">Biozen MagBeads</a> provide a fast and efficient method for purifying, isolating, and cleaning up monoclonal antibodies (mAbs), proteins, and peptides. These paramagnetic beads feature a streptavidin-coated surface, enabling high-affinity binding in immuno-based workflows. Compared to traditional sample preparation techniques, magnetic bead-based immunocapture offers greater speed and consistency by delivering high binding capacity with uniform particle size, ensuring accurate and reproducible results in significantly less time.</p><h5><strong>Centrifugation</strong></h5><p>Centrifugation of crude protein mixtures is an essential step in the investigation of protein purification and aggregation. Outlined below are the various types of centrifugations employed in protein purification.</p><p><strong>Preparative Ultracentrifugation</strong><br>Ultracentrifugation is an effective method of purifying proteins from aggregates based on their size, shape and density. Centrifugation, widely recognized as a robust and versatile method, is commonly used to separate complex mixtures of cellular components. Ultracentrifuges separate small particles by spinning at high speeds, utilizing centrifugal force based on size and density.</p><p><strong>Differential Centrifugation</strong><br>Differential centrifugation operates by separating components based on differences in size and density, using varying speeds of centrifugation to achieve this. Its resolving power ranges from low to medium. A key advantage of this technique is its convenience for processing multiple samples simultaneously, making it effective for purifying crude samples. Additionally, it preserves cell viability and maintains the natural distribution of hematopoietic cell populations.</p><p><strong>Rate-zonal Gradient Centrifugation</strong><br>This technique separates components based on their sedimentation rate within a medium that contains a density gradient. It offers medium resolving power and is particularly effective for separating multiple components in complex mixtures with high precision. It is also considered a cost-effective and widely used technique in molecular biology and biochemistry.</p><p><strong>Isopycnic Centrifugation (Equilibrium Centrifugation)</strong><br>Isopycnic centrifugation is an advanced protein purification method that relies on the principle of separating particles according to their intrinsic buoyant density, irrespective of their size or morphology. During the process, proteins migrate through a density gradient until they equilibrate at a position where their density matches that of the surrounding medium, resulting in precise separation without further sedimentation.</p></div></div></div><div><div><div><div><h4><strong>Chromatographic Approaches</strong></h4><p>Chromatography is a common method of protein purification and is based on the separation of molecules in a mixture as they move through a stationary phase and a mobile phase. Outlined below are the various types of chromatography employed in protein purification.</p><h5><strong>Ion Exchange Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange">Ion exchange chromatography</a> relies on the interaction between a charged stationary phase and oppositely charged analytes, with cation exchange chromatography attracting positively charged molecules to a negatively charged stationary phase. Successful application of this technique requires careful selection of the appropriate ion exchange column, buffer pH, and salt concentration. This approach is often used in the biopharmaceutical industries as mAb polishing steps, viral clearance and charge variant analyses as part of the product quality control.</p><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/">Phenomenex</a> offers a range of protein purification columns. Their ion exchange chromatography solutions include:&nbsp;</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1526"><strong>Luna SCX </strong></a>for strong selectivity of positively charged compounds, such as peptides</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1540"><strong>Biozen WCX</strong></a> for efficient separation of proteins such as monoclonal antibodies charged variants</li><li><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/part/00g-3151-e0">PhenoSphere SAX</a>, a strong anion-exchanger with fully porous 80Å particles, particularly useful for small molecules such as nucleotides</li></ul><h5><strong>Size Exclusion Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-sec">Size exclusion chromatography (SEC)</a> separates molecules based on their size, with larger analytes eluting first due to their exclusion from the pores in the column packing material, while smaller molecules are able to enter the pores, facing a longer travelling path, and hence, eluting later. This method can be categorized into aqueous SEC (gel filtration chromatography, GFC) for analyzing biomolecules such as proteins and antibodies. This type of chromatography is widely used as an analytical tool for quality control in the biopharmaceutical industry and can also be an approach of choice for polishing steps.</p><p>Phenomenex offers a range of <strong>Biozen columns for SEC</strong> separations. In the case of mAbs, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534"><strong>Biozen dSEC-2</strong></a> and<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1537"><strong> Biozen dSEC-7 </strong></a>columns are the top choices for monoclonal antibody (mAb) analysis, delivering high-resolution separation of monomers, aggregates, and fragments formed during mAb manufacturing, processing, or storage. The dSEC-7 column, featuring a larger pore size and optimized particle design, extends the dynamic range to accommodate larger biomolecules and complex biotherapeutics.</p><p>For more general applications, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1533"><strong>Biozen SEC-2</strong></a><strong> </strong>and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1532"><strong>Biozen SEC-3</strong></a> phases cover different MW ranges for high-efficiency separation of biomolecules (e.g., proteins and antibodies). Additionally, the BioSep and Yarra lines become top choices when looking for preparative scale columns.</p><h5><strong>Reversed-Phase Chromatography</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-reversed-phase">Reversed-phase chromatography</a>, the most common mode in high-performance liquid chromatography/ ultra-high-performance liquid chromatography (HPLC/UHPLC), separates compounds based on hydrophobic and van der Waals interactions using alkyl (C18, C8, C4) stationary phases or other hydrophobic polymers. For large biomolecules such as proteins and peptides, <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/blog/wider-pore-biozen-column">wide-pore</a> (typically 300 Å) C4, C8 and C18 columns are ideal, as the larger pore size allows better accessibility of the protein molecules to the stationary phase surface, improving mass transfer and peak shape during separation.</p><p>The Biozen line from Phenomenex offers a wide range of HPLC/UHPLC columns for the analysis of biomolecules.These include phases optimized for high-resolution analysis of intact proteins (Native-RP, Widepore C4, Intact XB-C8) and peptides (Peptide PS-C18, Peptide XB-C18 and Peptide Polar-C18). Kinetex could also be an option when looking for more selectivities for the analysis of peptides and small proteins. Additionally, when aiming to preparative scale processes, several of these media are also available in Phenomenex Axia formats to easily up-scale your separations.</p><h5><strong>Hydroxyapatite Chromatography</strong></h5><p>Hydroxyapatite chromatography (HAC) is a sophisticated mixed-mode chromatographic technique that exploits the unique properties of hydroxyapatite (Ca10(PO4)6(OH)2), utilizing both cation-exchange interactions via its phosphate groups and metal affinity through its calcium sites to achieve highly selective protein separations.</p><p>Renowned for its efficacy in resolving complex biomolecular separations, HAC excels in tasks like aggregate removal from monoclonal antibodies, surpassing the capabilities of traditional single-mode chromatographic methods such as ion-exchange or hydrophobic interaction chromatography.</p><h5><strong>Hydrophobic Interaction Chromatography</strong></h5><p>Hydrophobic interaction chromatography (HIC) is a method of protein purification that separates molecules based on their hydrophobicity, using high-salt buffers to promote protein-ligand interactions while maintaining the protein's biological activity. The process involves gradually reducing the salt concentration to elute proteins in order to increase hydrophobicity, making it effective for purifying target molecules and removing impurities or aggregates, making it a valuable approach for intermediate and polishing steps during purification processes.</p><h5><strong>Affinity Chromatography</strong></h5><p>Affinity chromatography is a powerful and highly selective technique used to purify biomolecules based on specific, reversible interactions between a target molecule and an immobilized ligand. In many cases, recombinant proteins are engineered with affinity tags—such as His-tags or GST-tags—that facilitate binding to complementary ligands on the chromatography matrix.</p><p>This method offers the advantage of achieving several thousand-fold purifications in a single step while preserving the biological activity of the protein. It is widely employed for purifying enzymes, antibodies, and antigens, using a variety of ligands including specific antibodies, enzymes, and multifunctional compounds like concanavalin A and triazine dyes.</p></div></div></div></div>

Exploring Protein Purification Methods: Essential Techniques for Accurate Results

<h3><span><strong>A Practical Guide to Getting the Most from Syringe Filters: Tips, Tricks, and Common Pitfalls</strong></span></h3><p><span><strong>Syringe filters play a crucial role in chromatography and many other laboratory applications by removing particulate matter before analysis. Proper sample filtration helps protect analytical instruments, extends column lifetime, and improves data quality. However, selecting an unsuitable filter—or using one incorrectly—can introduce contamination, reduce filtration efficiency, or even compromise analytical results. The following recommendations will help you avoid common mistakes and ensure reliable filtration every time.</strong></span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp" alt="KNAUER: Avoiding Common Syringe Filter Mistakes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_3_150e8c1c2b.webp 133w," sizes="100vw" width="1276" height="1496"></p><h4><span><strong>1. Improper Filter Handling</strong></span></h4><p><span>Using excessive force during filtration or attempting to reuse disposable syringe filters can damage the membrane and increase the risk of sample contamination.</span></p><ul><li><span><strong>Solution:</strong> Apply slow, even pressure throughout the filtration process and always dispose of single-use filters after one use. If the flow rate decreases noticeably, replace the filter instead of applying additional force.</span></li></ul><h4><span><strong>2. Selecting an Inappropriate Pore Size</strong></span></h4><p><span>Using the wrong pore size may either fail to remove unwanted particles or cause the membrane to clog prematurely, reducing filtration efficiency.</span></p><ul><li><span><strong>Solution:</strong> A 0.45 µm membrane is generally suitable for routine particulate removal, while a 0.22 µm membrane is recommended for finer filtration and bacterial removal. Samples with higher viscosity may require a pre-filter to prevent rapid clogging. It is also important to consider the intended analytical technique. For example, 0.22 µm filters are typically preferred for UHPLC and LC-MS applications, whereas 0.45 µm filters are often sufficient for conventional HPLC analyses.</span></li></ul><h4><span><strong>3. Choosing the Wrong Membrane Material</strong></span></h4><p><span>Different membrane materials exhibit different chemical resistance. Using an incompatible membrane may lead to degradation of the filter, sample contamination, or complete filtration failure.</span></p><ul><li><span><strong>Solution:</strong> Always verify solvent compatibility before filtration. PTFE membranes are an excellent choice for organic solvents, while PES and nylon membranes are better suited to aqueous samples. If compatibility is uncertain, perform a small-scale trial before filtering valuable samples.</span></li><li><span><strong>Pro Tip:</strong> For additional guidance, see our blog article <strong>"How to Choose the Right Membrane for your Syringe Filter"</strong>, which provides a detailed comparison of membrane materials and their recommended applications.</span></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg" alt="KNAUER Sepapure® Syringe Filters are available with multiple membrane materials, each identified by a dedicated color code for quick and reliable selection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_2_e47021fb11.jpg 245w," sizes="100vw" width="1580" height="902"></p><h4><span><strong>4. Ignoring Filter Capacity</strong></span></h4><p><span>Using a filter with insufficient capacity can result in rapid membrane blockage, increasing filtration time and reducing laboratory productivity.</span></p><ul><li><span><strong>Solution:</strong> When filtering larger sample volumes, choose high-capacity syringe filters or introduce a pre-filtration step. Never force liquid through a clogged membrane, as doing so may damage the filter and negatively affect sample quality.</span></li></ul><h4><span><strong>5. Overlooking Sample Adsorption</strong></span></h4><p><span>Certain membrane materials may adsorb analytes of interest, leading to lower recoveries and potentially affecting quantitative results.</span></p><ul><li><span><strong>Solution:</strong> For protein samples or other adsorption-sensitive analytes, select membranes with low binding characteristics, such as nylon or glass fiber, whenever appropriate. Performing a small recovery test before routine analysis can help identify potential analyte losses.</span></li></ul><h4><span><strong>6. Failing to Optimize Filtration Performance</strong></span></h4><p><span>Slow filtration is more than just an inconvenience—it often indicates that the filtration conditions are not optimal.</span></p><ul><li><span><strong>Solution:</strong> If filtration becomes unusually slow, investigate possible causes such as membrane clogging, excessive sample viscosity, or an unnecessarily small pore size. Depending on the sample, dilution or sequential filtration using progressively finer filters may significantly improve performance.</span></li></ul><h4><span><strong>Final Thoughts</strong></span></h4><p><span>Selecting the appropriate syringe filter is a straightforward process, but overlooking small details can have a significant impact on analytical performance. Choosing the correct membrane material, pore size, and filtration strategy helps improve laboratory efficiency, protects chromatography systems and columns, and contributes to accurate, reproducible analytical results.</span></p><p><span>With the right syringe filter and proper filtration technique, you can minimize sample preparation problems and maximize confidence in every analysis.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg" alt="KNAUER: Sepapure® Syringe Filter Selection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Avoiding_Common_Syringe_Filter_Mistakes_4_983a15e289.jpg 245w," sizes="100vw" width="1160" height="627"></p><p><span><strong>Happy filtering!</strong></span></p>

Avoiding Common Syringe Filter Mistakes

News from LabRulezLCMS Library - Week 27, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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<p><strong>Identifying small-molecule binders for complex biological targets remains a major challenge in early drug discovery, particularly when working with membrane proteins or large protein complexes. Mass spectrometry (MS)–based screening approaches are increasingly used to address these challenges, but they can present technical limitations and experimental design considerations.</strong></p><p>In this webinar, Dr. Johann Stojko, experienced biophysicist at Novalix, will present integrated workflows that combine covalent fragment screening and non-covalent binder detection to support hit discovery and validation.</p><p>Through practical case studies, attendees will see how complementary MS strategies can help improve confidence in screening results and accelerate progression from hit identification to lead optimization.</p><h4><strong>Attend this webinar to:</strong></h4><ul><li>Understand the screening approaches developed at Novalix</li><li>Explore workflows used to address challenging targets, including membrane proteins and protein complexes</li><li>Identify the limitations and advantages of each technique</li><li>Learn how experimental design and appropriate controls can improve confidence in MS screening results</li></ul><blockquote><p><strong>Speaker:&nbsp;</strong>Johann Stojko, PhD (Senior Project Manager, Novalix)<br><br><strong>Speaker:&nbsp;</strong>François Debaene (Head of Mass Spectrometry, Novalix)</p></blockquote>

Technology Networks: Mass Spectrometry in Drug Discovery: Covalent and Non-Covalent Screening Applications

Mass Spectrometry in Drug Discovery: Covalent and Non-Covalent Screening Applications

Integrated workflows that combine covalent fragment screening and non-covalent binder detection to support hit discovery and validation.

Covalent and Non-Covalent Screening Applications

<p><strong>Crosslinking mass spectrometry (XL-MS) is transforming how researchers map protein–protein and protein–nucleic acid interactions, delivering structural insights at peptide-level resolution. But achieving high-confidence results requires an optimized, end-to-end workflow.</strong></p><p>Join us for this webinar to learn how to implement practical XL-MS strategies that improve crosslink detection, sensitivity, and data confidence. Our expert speaker will walk through best practices across sample preparation, advanced LC-MS acquisition, and data analysis using Thermo Scientific™ Proteome Discoverer™ software with the XlinkX algorithm.</p><h4><strong>You Will Learn</strong></h4><ul><li>How to optimize sample preparation and enrichment strategies to enhance crosslink identification</li><li>Advanced LC-MS acquisition strategies, including real-time library search (RTLS) for improved sensitivity</li><li>Best practices for analyzing crosslinking data in Proteome Discoverer software with XlinkX</li><li>Approaches for visualizing and interpreting interaction data to support structural insights</li><li>Introduction to NuXL workflows for profiling protein–nucleic acid interactions and expanding interactomics capabilities</li></ul><blockquote><p><strong>Speaker: </strong>Yi He (Marketing Manager, Thermo Fisher Scientific)</p><p>Dr. Yi He earned her PhD in Analytical Chemistry from Indiana University Bloomington. She was a research fellow and instructor at the University of Washington, applying structural proteomics to cardiovascular disease and diabetes research. In 2022, she joined Thermo Fisher Scientific as Vertical Marketing Manager for Structural Biology, focusing on crosslinking MS, protein structure, and protein–nucleic acid interactions.</p><p><strong>Moderator: </strong>Weijing Liu (Senior Marketing Manager, Thermo Fisher Scientific)</p></blockquote>

Thermo Fisher Scientific: From Sample to Structure: Crosslinking MS Made Practical

From Sample to Structure: Crosslinking MS Made Practical - Part 1

Learn how to implement practical XL-MS strategies that improve crosslink detection, sensitivity, and data confidence.

Crosslinking MS Made Practical

<p><strong>Purifying synthetic peptides is a critical step in developing advanced biotherapeutics like GLP-1 receptor agonists. Even with precise synthesis, impurities such as deletions, truncations, and chemical modifications are unavoidable, making purification essential for achieving high purity, optimal yield, and cost efficiency.</strong></p><p>Join this SelectScience® webinar to discover why reversed-phase chromatography (RP) remains the gold standard for peptide purification and analysis. This session will be led by Bill Warren, Principal Consultant Product Manager, Waters Corporation, who has extensive experience with chromatography techniques for protein purification. He will guide you through the complete lab-scale purification process for a GLP-1 RA using optical detection, and will show how these strategies can be applied to other synthetic peptide modalities, including radio-labeled peptides, cyclic peptides, peptide antimicrobials, and more.</p><h4><strong>Key learning objectives:</strong></h4><ul><li>Understand strategies for developing analytical scale biotherapeutic peptide separations.</li><li>Learn techniques for lab-scale preparative synthetic peptide separations and fraction analysis.</li><li>Explore how reversed-phase mobile phase modifiers and chemistries impact peptide separation performance.</li><li>Gain practical troubleshooting tips for reversed-phase peptide separations.</li></ul><h4><strong>Who should attend?</strong></h4><ul><li>Biopharmaceutical and peptide research professionals, including academics and industry scientists.</li><li>Biopharma outsourcing partners, such as CDMOs, CMOs, and CROs.</li><li>Broader biopharmaceutical industry professionals involved in development, manufacturing, or research</li></ul><blockquote><p><strong>Speaker:&nbsp;</strong>Bill Warren (Principal Consultant Product Manager, Waters Corporation)</p><p>Bill Warren joined Waters Corporation as a senior applications chemist involved with the research and development of HPLC, FPLC, and capillary electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids. He now acts as Principal Consultant Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of biological molecules.</p><p><strong>Moderator: </strong>Olivia Long (Editorial Team, SelectScience)</p></blockquote>

Select Science: Why researchers turn to reversed phase chromatography when purifying GLP-1

Why researchers turn to reversed phase chromatography when purifying GLP-1

Understand strategies for developing analytical scale biotherapeutic peptide separations.

Why researchers turn to reversed phase chromatography

<p><strong>Along with gravimetry, titration is one of the oldest and most reliable quantitative analytical methods known. It is generally used as the primary reference method for other, mostly qualitative, analytical methods and it is indispensable for routine analysis in the laboratory.</strong></p><p>In this webinar, Robert Tobler, Senior Application Specialist Titration at Metrohm International Headquarters, will give you an introduction to the basics of titration. After a brief excursion into the theory, Robert will explain how to perform a titration step by step on a modern, automatic titrator with a photometric or an electrochemical sensor.</p><p>During a Q&amp;A session at the end of the webinar you can ask Robert any question you may have about titration. Join us on 29 April; we are looking forward to welcoming you in this webinar!</p><h4><strong>Key learnings of this webinar:</strong></h4><ul><li>The most important measuring modes for potentiometric titration and application examples from various industries</li><li>How to optimize your titration results for higher accuracy and better reproducibility</li><li>Common mistakes and how you can avoid them</li><li>How to properly handle and take care of your electrodes</li></ul><h4><strong>Who should attend?</strong></h4><ul><li>Lab technicians, chemists, and everybody else using titration for their routine analytics.</li></ul><blockquote><p><strong>Presenter: </strong>Robert Tobler (Senior Application Specialist Titration, Metrohm International Headquarters)</p><p>After his apprenticeship as a chemical laboratory technician and further training as a biomedical analyst, Robert Tobler studied chemistry at the Zurich University of Applied Science in Waedenswil, Switzerland. He joined Metrohm in 2015 as an application specialist titration. Since then, Robert has become an expert in applied titration sharing his expertise through the global network of Metrohm subsidiaries.</p></blockquote>

Metrohm: The Basics of Titration explained

The Basics of Titration explained

In this webinar, Robert Tobler will give you an introduction to the basics of titration.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Join us for two enlightening webinars aimed at advancing your laboratory work! These sessions are tailored for professionals navigating regulations such as pharmacopoeia and American Chemical Society (ACS) guidelines, or those working in QC labs where precision is paramount. Learn how to implement PFAS-tested consumables and compliant reagents can refine your analytical processes.</strong></p><ul><li><strong>April 28: Understanding Pharmacopoeia and ACS Compliance</strong><br>Discover the critical role of compliant reagents in your lab and learn how to ensure that your analyses meet regulatory standards.&nbsp;</li><li><strong>April 30: Benefits of PFAS-Tested Consumables</strong><br>Gain insights into the importance of PFAS-tested additives and solvents for reliable analysis and their impact on the quality of your results.</li></ul><p><i><strong>*Two sessions per day: Europe/Asia &amp; Americas time zones</strong></i></p><h4><strong>What You'll Gain</strong></h4><p>Analytical Excellence Week 2026 is meticulously organized to empower professionals in analytical chemistry, particularly those in regulated pharmaceutical labs, industrial quality control labs, environmental labs, and other testing labs. This is your opportunity to hear from industry experts sharing valuable knowledge and best practices for better workflow strategies.</p><h4><strong>April 30: Benefits of PFAS-Tested Consumables</strong></h4><ul><li>Gain a clear understanding of PFAS regulatory guidelines</li><li>Learn the role of high-purity solvents and additives in preventing contamination</li><li>Ensure accurate and reliable PFAS analytical results</li></ul><h4><strong>Who Should Attend:</strong></h4><p>All professionals involved in pharmaceutical or chemical analysis laboratories. You'll take away actionable insights that can immediately improve your daily practices.</p><p><i>Don't miss out on this chance to achieve greater analytical outcomes. Register now!</i></p><h5><strong>Broadcasts</strong></h5><ul><li><strong>Europe / Asia Broadcast: </strong>8 am BST (London) / &nbsp;9 am CEST (Paris/Berlin) / 12:30 pm IST (Mumbai) / 3 pm CST (Beijing) &nbsp;/ 4 pm JST (Tokyo)</li><li><strong>Americas Broadcast:</strong> 9 am PDT (Los Angeles) / 11 am CDT (Chicago) / 12 pm EDT (New York) &nbsp;</li><li><strong>Duration: </strong>Approximately 1 hour each day</li></ul><blockquote><p><strong>Presenter: </strong>Bettina Straub-Jubb (Senior Global Product Manager Analytical Technolgies, Merck KGaA, Darmstadt, Germany)</p><p>Bettina is a Senior Global Product Manager and Lead Expert for Titration at Merck KGaA, Darmstadt, Germany. With over 30 years of experience in instrumental analysis, diagnostics, and reference materials, she specializes in titration and Karl Fischer titration. She is dedicated to enhancing and streamlining titration processes, driving the development of innovative technologies, advancing applications and reagents, and providing expert support to both customers and colleagues.</p><p>She works closely with quality control and application laboratories, as well as the Research and Development department, to ensure continuous improvement in titration methodologies. Bettina holds a degree in Chemical Technology from the University of Applied Sciences, Darmstadt, Germany, and is a qualified Medical Technical Laboratory Assistant from the Medical Technical Academy Esslingen, Germany.</p><p><strong>Presenter:&nbsp;</strong>Christian Roth (Senior Global Product Manager Inorganic Reagents, Merck KGaA, Darmstadt, Germany)</p><p>Christian Roth is a Senior Product Manager for Classical Inorganics at Merck KGaA, Darmstadt, Germany, where he has dedicated over 25 years to managing a diverse portfolio that includes salts, acids, caustic alkalis, bases, metals, and metal oxides of analytical purity.</p><p>With a degree in International Business Management, Christian combines his extensive technical knowledge with a strong foundation in global business strategies. His expertise ensures that the products under his purview not only meet but exceed the rigorous requirements of analytical laboratories, addressing critical chemical, regulatory, and safety standards, and ultimately delivering exceptional value to customers.</p><p><strong>Presenter:&nbsp;</strong>Dr. Patrik Appelblad (Fellow and Technical lead, Chemistry R&amp;D, &nbsp;Merck KGaA, Darmstadt, Germany)</p><p>Patrik is passionate about simplifying science, advancing instrumental analysis, assisting the scientific community in solving problems, as well as sharing knowledge through modern channels and diverse methods. Holding a PhD in Analytical Chemistry from Umea University, Sweden, Patrik brings almost 25 years of experience from various R&amp;D and marketing roles within the company. This extensive background equips him well as a technical lead and spokesperson on a range of topics, including PFAS Testing.</p><p><strong>Presenter:&nbsp;</strong>Patric Klein (Senior Global Product Manager; Organic and Inorganics Instrumental Analysis, Merck KGaA, Darmstadt, Germany)</p><p>Patric Klein is a Senior Global Product Manager for Organic and Inorganic Reagents for Instrumental Analysis at Merck KGaA, Darmstadt, Germany. With over 20 years of experience at Merck, he has developed a strong expertise in managing high-purity reagents for instrumental analysis. His focus includes high-purity acids for AAS and ICP applications, as well as high-purity reagents for chromatography techniques such as IC, HPLC and LC-MS. Patric holds a Master's degree in Chemical Engineering which underpins his extensive knowledge and contributions to the field.</p><p><strong>Presenter:&nbsp;</strong>Lena Werner (Senior Global Product Manager Specialty Solvents, Merck KGaA, Darmstadt, Germany)</p><p>Lena Werner is a Senior Global Product Manager for Specialty Solvents at Merck Life Science KGaA Darmstadt with nearly 20 years of experience in analytical solvents. She manages specialty solvents, including anhydrous, deuterated and PFAS-tested solvents. Lena holds a degree in Business Administration from Baden-Wuerttemberg Cooperative State University Mannheim, focusing on marketing, production, and logistics. At Merck, she has managed various product portfolios, including managing classical solvents for quality control and high-purity solvents for gas chromatography and spectroscopy.</p></blockquote></div></div></div>

SeparationScience: Analytical Excellence Week 2026

Analytical Excellence Week 2026: Part 2 - Benefits of PFAS-Tested Consumables

Gain insights into the importance of PFAS-tested additives and solvents for reliable analysis and their impact on the quality of your results.

Benefits of PFAS-Tested Consumables

<div><p><strong>Integrating MALDI Imaging with spatial transcriptomics enables a more complete understanding of tissue organization by combining molecular distributions with high resolution gene expression patterns on the same tissue section. This multimodal approach provides deeper insight into cellular heterogeneity, functional states, and microenvironmental interactions, surpassing what either technique can reveal alone.</strong></p><p>This webinar brings together two perspectives that complete each other: a methodological view on how MALDI Imaging and spatial transcriptomics can be successfully integrated on a single tissue section, and an application focused perspective illustrating what new biological insights emerge once both data layers are aligned. Advances in sample compatibility, spatial coregistration, and data integration provide the technical foundation, while multimodal analyses of complex tissues reveal subtle molecular gradients, cell state variation, and region specific features relevant to disease mechanisms.</p><p>More broadly, linking biochemical phenotypes with transcriptional programs supports improved interpretation of complex tissues, biomarker discovery, and mechanistic studies across spatial biology, demonstrating how methodological innovation and biological application jointly enable deeper scientific insight.</p><h4><strong>Key Learning Objectives</strong></h4><ul><li>Understand why integrating MALDI Imaging with spatial transcriptomics provides deeper biological context than either modality alone</li><li>Gain insight into how multimodal workflows can be implemented in practice</li><li>Learn how multimodal spatial readouts help uncover disease relevant molecular patterns and cellular heterogeneity</li><li>Discover how integrating molecular and transcriptomic information supports biomarker research and translational applications</li><li>See how complementary spatial readouts contribute to advanced applications across neuroscience, oncology, and broader spatial biology</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>Researchers in spatial biology, and multimodal imaging</li><li>Scientists studying tissue‑level molecular changes</li><li>Imaging mass spectrometry, transcriptomics, and single‑cell specialists exploring integrated workflows</li><li>Anyone interested in how MALDI Imaging contributes to next‑generation spatial multiomics and respective study design</li></ul><blockquote><p><strong>Presenter:</strong> Eva Cuypers, Associate Professor, M4i - Maastricht for Imaging, Maastricht, Netherlands</p></blockquote></div>

Bruker: Bridging Molecules and Genes: Integrating MALDI Imaging and Spatial Transcriptomics on a Single Tissue Section

Bridging Molecules and Genes: Integrating MALDI Imaging and Spatial Transcriptomics on a Single Tissue Section

Learn how MALDI Imaging and spatial transcriptomics integration enables multimodal tissue analysis, biomarker discovery, and advanced spatial biology insights.

Integrating MALDI Imaging and Spatial Transcriptomics

<p><strong>On April 28th, Corey Meadows, Commercial Product Manager - Analytical Solutions at Tosoh Bioscience, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts. Landry Bennett, Global Technical Sales Specialist - Instrumentation at Tosoh Bioscience for the LenS3 MALS detector, will introduce light scattering theory and discuss practically implementing MALS.&nbsp;</strong></p><p>Landry will also share some selected applications to show where MALS detectors earn their value. Participants will learn how MALS detectors work, some best-practices for running SEC-MALS experiments, and how to make the most of using MALS detectors both polymer and biologics characterization.</p><p><strong>After the webinar, ask your questions during the live Q&amp;A session.&nbsp;</strong></p><blockquote><p><strong>Presenter: </strong>Corey Meadows, Commercial Product Manager - Analytical Solutions, Tosoh Bioscience</p><p><strong>Presenter:</strong> Landry Bennett, Global Technical Sales Specialist - Instrumentation, Tosoh Bioscience</p></blockquote>

Tosoh Bioscience: Tosoh Tuesday - What is MALS & When is it Useful?

Tosoh Tuesday - What is MALS & When is it Useful?

Learn MALS theory, SEC-MALS best practices, and applications in polymer and biologics analysis with Tosoh Bioscience experts in this webinar.

What is MALS & When is it Useful

<p><strong>Solving complex analytical challenges with QTRAP® workflows</strong><br><strong>Modern analytical laboratories face increasing complexity — from trace‑level quantitation to challenging matrices, demanding regulations, and the need for unequivocal confirmation. Traditional triple quadrupole systems are often pushed to their limits.</strong></p><p>In this webinar, we introduce how QTRAP® transforms LC-MS/MS workflows by combining high-performance quantitation with unique MS³-level selectivity and full-scan spectral confirmation.</p><p>Using real-world application examples—including polar pesticides, multiresidue pesticides, PFAS, mycotoxins, veterinary drugs, and forensic toxicology—we will demonstrate how QTRAP® workflows such as MRM³ and MRM-IDA-EPI deliver unmatched selectivity, eliminate interference, reduce background noise, and achieve LOQs not possible with conventional MRM.</p><p><strong>If you’ve ever wished for “more than a triple quad,” this session is for you.</strong></p><h4><strong>What to expect:</strong></h4><p>During this webinar, attendees will:</p><ul><li>See side‑by‑side MRM vs. MRM³ chromatograms across many matrices</li><li>Understand when MRM is not enough — and how QTRAP® solves it</li><li>Learn how laboratories improved LOQs, selectivity, and confidence</li><li>Discover workflows that combine quantitation, confirmation, and screening in a single run</li><li>Receive a practical framework to assess which analytes benefit from QTRAP® functionalities</li></ul><h4><strong>Key learning points:</strong></h4><p>Participants will learn:</p><ul><li>QTRAP® overview</li><li>When and why MRM³ makes a difference</li><li>Real‑life application workflows</li><li>Practical method development tips</li></ul><h4><strong>Who should attend:</strong></h4><p>This webinar is designed for professionals working with complex LC‑MS/MS analyses, including:</p><ul><li>Food safety laboratories</li><li>Environmental laboratories</li><li>Forensic and toxicology labs</li><li>Analytical scientists and method developers</li><li>Research labs</li><li>Anyone using LC‑MS/MS who faces interferences, poor selectivity, or challenging matrices</li></ul><p><i>We will send you the recording after the event.</i></p><blockquote><p><strong>Speaker:</strong> Dr. Jianru Stahl-Zeng</p><p>Jianru is Senior Technical Marketing Manager. She has been part of SCIEX since 2001. She initially was part of the application support team and covered not only proteomics, but also small molecules applications. Jianru moved to technical marketing and focused on food and environmental applications. Since 2018 she has been managing the EMEA technical marketing team and projects. Jianru is also the Global Technical Leader for food and environmental applications.</p></blockquote>

SCIEX: Extending the power of LC‑MS/MS: advanced workflows with QTRAP®

Extending the power of LC‑MS/MS: advanced workflows with QTRAP®

How QTRAP LC-MS/MS workflows (MRM³, MRM-IDA-EPI) improve selectivity, lower LOQs, and deliver reliable results for food, environmental, and forensic analysis.

Power of LC‑MS/MS: advanced workflows with QTRAP

<p><strong>Learn the essentials of Size Exclusion Chromatography (SEC) for biopharma - a key technique for assessing molecular size, detecting aggregates, and evaluating product purity and stability.</strong></p><p>In this Fundamentals First Bio Series webinar, we’ll cover core SEC principles, instrumentation and column considerations, and best practices for method development and troubleshooting strategies.</p><p>Through real-world examples - including monoclonal antibodies, biosimilars, AAVs, and nucleic acids - attendees will gain practical insights to improve method performance and confidence in their analytical results.</p><h5><strong>Key Learning Points</strong></h5><ul><li>Understand core SEC principles and key terminology.</li><li>Select appropriate instrumentation, columns, and method conditions.</li><li>Apply practical method optimization and troubleshooting best practices.</li><li>Review of common SEC applications for biologics characterization.</li></ul><h5><strong>Who Should Attend</strong></h5><ul><li>Analytical scientists and researchers working in biopharma development.</li><li>Process development and QC teams supporting biomolecule characterization.</li><li>Scientists seeking a foundational understanding of SEC methods and best practices.</li></ul><blockquote><p><strong>Speaker:</strong> Zach Woodward (Senior Technical Specialist, Phenomenex)</p><p>Zachary Woodward is a Senior Technical Specialist with Phenomenex, Inc, assisting laboratories with method development in the bio-pharma, environmental, and food industries. Zachary spent his formative years developing HPLC and GC methods with a pharmaceutical lab in Albany, NY, after which he developed HPLC and SPE methods in a clinical setting here in Southern California. Zachary received his B.Sci. in Chemistry from the College of William and Mary, was awarded his Master’s in Chemistry at San Diego State University, and is happy to speak with you today!</p></blockquote>

Phenomenex: Fundamentals First Bio Series – SEC for Biologics

Fundamentals First Bio Series – SEC for Biologics

Learn SEC fundamentals for biopharma, including method development, column selection, and applications in biologics characterization like mAbs and AAVs.

<p><strong>Discover how LC-MS is advancing biopharma workflows through scalable, high-throughput proteomics. This webinar will highlight recent developments in LC-MS-based approaches, including HCP analysis, untargeted quantification strategies, and scalable sample preparation, with a focus on robustness, reproducibility, and applicability in both research and regulated quality control environments.</strong></p><h5><strong>Prospective ICH Q2(R2)-aligned total-error validation of label-free untargeted proteomics for host cell protein quantification in biotherapeutics</strong></h5><ul><li>Somar khalil, PhD, Principal Scientist, Analytical research &amp; Development, GSK</li></ul><p>Abstract to be announced</p>

Evosep: Next-Gen HCP Analysis in Biopharma

Next-Gen HCP Analysis in Biopharma

Discover how LC-MS is advancing biopharma workflows through scalable, high-throughput proteomics. 